Gene Locus : torca-ACHE
Mode of mutation : Site directed mutagenesis
Disease :
Summary : Acylation increases Km and Kss and reduces kcat Gibney_1990_Proc.Natl.Acad.Sci.U.S.A_87_7546 Radic_1992_Biochemistry_31_9760 Saxena_1993_Biochem.Biophys.Res.Commun_197_343 || Acylation,Phosphorylation Decreased affinity for reversible inhibitors and reduced rates of phosphorylation carbamylation Gibney_1990_Proc.Natl.Acad.Sci.U.S.A_87_7546 Radic_1992_Biochemistry_31_9760 Saxena_1993_Biochem.Biophys.Res.Commun_197_343 || Acylation,Phosphorylation resistant to aging Saxena_1993_Biochem.Biophys.Res.Commun_197_343 || Huperzine A 3-fold increase in KI value for the binding of Huperzine A Saxena_1994_Protein.Sci_3_1770
AAA Change :
Allelic Variant :
Risk Factor :
Inhibitor :
Structure :
Disease by interaction :
Interact Gene Locus :
Xenobiotic sensitivity :
Modification : Acylation,Phosphorylation
Torpedo_number : 199
Kinetic Parameter : No kinetic parameter
News : No news
Comment :
p.Glu199Gln
| Title : Mutant acetylcholinesterases as potential detoxification agents for organophosphate poisoning - Saxena_1997_Biochem.Pharmacol_54_269 |
| Author(s) : Saxena A , Maxwell DM , Quinn DM , Radic Z , Taylor P , Doctor BP |
| Ref : Biochemical Pharmacology , 54 :269 , 1997 |
| Abstract : |
| PubMedSearch : Saxena_1997_Biochem.Pharmacol_54_269 |
| PubMedID: 9271331 |
| Title : The Function of Electrostatics in Acetylcholinesterase Catalysis - |
| Author(s) : Quinn DM , Seravalli J , Nair HK , Medhekar R , Husseini B , Radic Z , Vellom DC , Pickering NA , Taylor P |
| Ref : In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases , (Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp. :203 , 1995 |
| PubMedID: |
| Title : Identification of amino acid residues involved in the binding of Huperzine A to cholinesterases - Saxena_1994_Protein.Sci_3_1770 |
| Author(s) : Saxena A , Qian N , Kovach IM , Kozikowski AP , Pang YP , Vellom DC , Radic Z , Quinn DM , Taylor P , Doctor BP |
| Ref : Protein Science , 3 :1770 , 1994 |
| Abstract : |
| PubMedSearch : Saxena_1994_Protein.Sci_3_1770 |
| PubMedID: 7849595 |
| Title : The role of glutamate-199 in the aging of cholinesterase - Saxena_1993_Biochem.Biophys.Res.Commun_197_343 |
| Author(s) : Saxena A , Doctor BP , Maxwell DM , Lenz DE , Radic Z , Taylor P |
| Ref : Biochemical & Biophysical Research Communications , 197 :343 , 1993 |
| Abstract : |
| PubMedSearch : Saxena_1993_Biochem.Biophys.Res.Commun_197_343 |
| PubMedID: 7902714 |
| Title : Expression of recombinant acetylcholinesterase in a baculovirus system: kinetic properties of glutamate 199 mutants - Radic_1992_Biochemistry_31_9760 |
| Author(s) : Radic Z , Gibney G , Kawamoto S , MacPhee-Quigley K , Bongiorno C , Taylor P |
| Ref : Biochemistry , 31 :9760 , 1992 |
| Abstract : |
| PubMedSearch : Radic_1992_Biochemistry_31_9760 |
| PubMedID: 1356436 |
| Title : Mutagenesis of essential functional residues in acetylcholinesterase - Gibney_1990_Proc.Natl.Acad.Sci.U.S.A_87_7546 |
| Author(s) : Gibney G , Camp S , Dionne M , MacPhee-Quigley K , Taylor P |
| Ref : Proceedings of the National Academy of Sciences of the United States of America , 87 :7546 , 1990 |
| Abstract : |
| PubMedSearch : Gibney_1990_Proc.Natl.Acad.Sci.U.S.A_87_7546 |
| PubMedID: 2217185 |