Gene Locus : mouse-ACHE
Mode of mutation : Site directed mutagenesis
Disease :
Summary : Acyl specificity Acyl pocket, confers butyrylcholinesterase activity Vellom_1993_Biochemistry_32_12 Pickering_1995_5th.ChE.Meeting.Madras__227 || Fasciculin inhibition low effect Radic_1994_J.Biol.Chem_269_11233 || Aricept~Donepezil~E2020 inhibition increase Ki vs WT Saxena_2003_Eur.J.Biochem_270_4447 || Oxime interaction enlargement of the active site of the gorge Kovarik_2006_Biochem.Biophys.Res.Commun_342_973 Kovarik_2007_Toxicology_233_79
AAA Change :
Allelic Variant :
Risk Factor :
Inhibitor :
Structure :
Disease by interaction :
Interact Gene Locus :
Xenobiotic sensitivity :
Modification : Acyl specificity || Aricept~Donepezil~E2020 inhibition || Oxime interaction || Fasciculin inhibition
Torpedo_number : 288
Kinetic Parameter : Acetylthiocholine_F295L_mouse-ACHE, WT-fasciculin2_F295L_mouse-ACHE, Butyrylthiocholine_F295L_mouse-ACHE, Benzoylthiocholine_F295L_mouse-ACHE
News : No news
Comment :
enlargement of the active site of the gorge || p.Phe295Leu
| Title : Mutation of acetylcholinesterase to enhance oxime-assisted catalytic turnover of methylphosphonates - Kovarik_2007_Toxicology_233_79 |
| Author(s) : Kovarik Z , Radic Z , Berman HA , Taylor P |
| Ref : Toxicology , 233 :79 , 2007 |
| Abstract : |
| PubMedSearch : Kovarik_2007_Toxicology_233_79 |
| PubMedID: 17046138 |
| Title : Active site mutant acetylcholinesterase interactions with 2-PAM, HI-6, and DDVP - Kovarik_2006_Biochem.Biophys.Res.Commun_342_973 |
| Author(s) : Kovarik Z , Ciban N , Radic Z , Simeon-Rudolf V , Taylor P |
| Ref : Biochemical & Biophysical Research Communications , 342 :973 , 2006 |
| Abstract : |
| PubMedSearch : Kovarik_2006_Biochem.Biophys.Res.Commun_342_973 |
| PubMedID: 16598855 |
| Title : Aromatic amino-acid residues at the active and peripheral anionic sites control the binding of E2020 (Aricept) to cholinesterases - Saxena_2003_Eur.J.Biochem_270_4447 |
| Author(s) : Saxena A , Fedorko JM , Vinayaka CR , Medhekar R , Radic Z , Taylor P , Lockridge O , Doctor BP |
| Ref : European Journal of Biochemistry , 270 :4447 , 2003 |
| Abstract : |
| PubMedSearch : Saxena_2003_Eur.J.Biochem_270_4447 |
| PubMedID: 14622273 |
| Title : Inactivation studies of acetylcholinesterase with phenylmethylsulfonyl fluoride - Kraut_2000_Mol.Pharmacol_57_1243 |
| Author(s) : Kraut D , Goff H , Pai RK , Hosea NA , Silman I , Sussman JL , Taylor P , Voet JG |
| Ref : Molecular Pharmacology , 57 :1243 , 2000 |
| Abstract : |
| PubMedSearch : Kraut_2000_Mol.Pharmacol_57_1243 |
| PubMedID: 10825396 |
| Title : Specificity and orientation of trigonal carboxyl esters and tetrahedral alkylphosphonyl esters in cholinesterases - Hosea_1995_Biochemistry_34_11528 |
| Author(s) : Hosea NA , Berman HA , Taylor P |
| Ref : Biochemistry , 34 :11528 , 1995 |
| Abstract : |
| PubMedSearch : Hosea_1995_Biochemistry_34_11528 |
| PubMedID: 7547883 |
| Title : Organophosphate Specificity of Acyl Pocket Cholinesterase Mutants - |
| Author(s) : Pickering NA , Taylor P , Berman HA |
| Ref : In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases , (Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp. :227 , 1995 |
| PubMedID: |
| Title : Site of fasciculin interaction with acetylcholinesterase - Radic_1994_J.Biol.Chem_269_11233 |
| Author(s) : Radic Z , Duran R , Vellom DC , Li Y , Cervenansky C , Taylor P |
| Ref : Journal of Biological Chemistry , 269 :11233 , 1994 |
| Abstract : |
| PubMedSearch : Radic_1994_J.Biol.Chem_269_11233 |
| PubMedID: 8157652 |
| Title : Amino acid residues controlling acetylcholinesterase and butyrylcholinesterase specificity - Vellom_1993_Biochemistry_32_12 |
| Author(s) : Vellom DC , Radic Z , Li Y , Pickering NA , Camp S , Taylor P |
| Ref : Biochemistry , 32 :12 , 1993 |
| Abstract : |
| PubMedSearch : Vellom_1993_Biochemistry_32_12 |
| PubMedID: 8418833 |
| Title : Three distinct domains in the cholinesterase molecule confer selectivity for acetyl- and butyrylcholinesterase inhibitors - Radic_1993_Biochemistry_32_12074 |
| Author(s) : Radic Z , Pickering NA , Vellom DC , Camp S , Taylor P |
| Ref : Biochemistry , 32 :12074 , 1993 |
| Abstract : |
| PubMedSearch : Radic_1993_Biochemistry_32_12074 |
| PubMedID: 8218285 |