6S97
Name : Fragment transplantation onto a hyperstable ancestor of haloalkane dehalogenases and Renilla luciferase (Anc-FT) AncFT
Revelation date : 26-Aug-2020
Family : Haloalkane_dehalogenase-HLD2
Gene_locus : 9zzzz-AncHLDRLuc2
PDB file : ESTHER: header of PDB entry RCSB: Full entry
Comment
Schenkmayerova, A., Damborsky, J., Marek, M. || Luciferase from Renilla reniformis (RLuc)<\/A> catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) Reconstruction of an ancestral enzyme shows it has both hydrolase and monooxygenase activities ( Chaloupkova et al.<\/A>) Preprint available of: Engineering Protein Dynamics of Ancestral Luciferase DOI: 10.26434\/chemrxiv.12808295
Ligand :
References (2)
| Title : Engineering the protein dynamics of an ancestral luciferase - Schenkmayerova_2021_Nat.Commun_12_3616 |
| Author(s) : Schenkmayerova A , Pinto GP , Toul M , Marek M , Hernychova L , Planas-Iglesias J , Daniel Liskova V , Pluskal D , Vasina M , Emond S , Dorr M , Chaloupkova R , Bednar D , Prokop Z , Hollfelder F , Bornscheuer UT , Damborsky J |
| Ref : Nat Commun , 12 :3616 , 2021 |
| Abstract : Schenkmayerova_2021_Nat.Commun_12_3616 |
| ESTHER : Schenkmayerova_2021_Nat.Commun_12_3616 |
| PubMedSearch : Schenkmayerova_2021_Nat.Commun_12_3616 |
| PubMedID: 34127663 |
| Gene_locus related to this paper: renre-luc |
| Title : Engineering Protein Dynamics of Ancestral Luciferase - Schenkmayerova_2020_Chemrxiv__ |
| Author(s) : Schenkmayerova A , Pinto GP , Toul M , Marek M , Hernychova L , Planas-Iglesias J , Liskova V , Pluskal D , Vasina M , Emond S , Dorr M , Chaloupkova R , Bednar D , Prokop Z , Hollfelder F , Bornscheuer UT , Damborsky J |
| Ref : Chemrxiv , : , 2020 |
| Abstract : Schenkmayerova_2020_Chemrxiv__ |
| ESTHER : Schenkmayerova_2020_Chemrxiv__ |
| PubMedSearch : Schenkmayerova_2020_Chemrxiv__ |
| PubMedID: |
| Gene_locus related to this paper: renre-luc |
Representative scheme of Prolylcarboxypeptidase structure and an image from PDBsum server
Databases
PDB-Sum : 6S97 Previously Class, Architecture, Topology and Homologous superfamily - PDB-Sum server
FSSP : 6S97 Fold classification based on Structure-Structure alignment of Proteins - FSSP server
SCOP : 6S97 Structural Classification Of Protein - SCOP server
PROTEOPEDIA : 6S97 3D, interactive encyclopedia of proteins - PROTEOPEDIA server