Kramer M

References (5)

Title : Mutations in the pancreatic secretory enzymes CPA1 and CPB1 are associated with pancreatic cancer - Tamura_2018_Proc.Natl.Acad.Sci.U.S.A_115_4767
Author(s) : Tamura K , Yu J , Hata T , Suenaga M , Shindo K , Abe T , MacGregor-Das A , Borges M , Wolfgang CL , Weiss MJ , He J , Canto MI , Petersen GM , Gallinger S , Syngal S , Brand RE , Rustgi A , Olson SH , Stoffel E , Cote ML , Zogopoulos G , Potash JB , Goes FS , McCombie RW , Zandi PP , Pirooznia M , Kramer M , Parla J , Eshleman JR , Roberts NJ , Hruban RH , Klein AP , Goggins M
Ref : Proc Natl Acad Sci U S A , 115 :4767 , 2018
Abstract : To evaluate whether germline variants in genes encoding pancreatic secretory enzymes contribute to pancreatic cancer susceptibility, we sequenced the coding regions of CPB1 and other genes encoding pancreatic secretory enzymes and known pancreatitis susceptibility genes (PRSS1, CPA1, CTRC, and SPINK1) in a hospital series of pancreatic cancer cases and controls. Variants in CPB1, CPA1 (encoding carboxypeptidase B1 and A1), and CTRC were evaluated in a second set of cases with familial pancreatic cancer and controls. More deleterious CPB1 variants, defined as having impaired protein secretion and induction of endoplasmic reticulum (ER) stress in transfected HEK 293T cells, were found in the hospital series of pancreatic cancer cases (5/986, 0.5%) than in controls (0/1,045, P = 0.027). Among familial pancreatic cancer cases, ER stress-inducing CPB1 variants were found in 4 of 593 (0.67%) vs. 0 of 967 additional controls (P = 0.020), with a combined prevalence in pancreatic cancer cases of 9/1,579 vs. 0/2,012 controls (P < 0.01). More ER stress-inducing CPA1 variants were also found in the combined set of hospital and familial cases with pancreatic cancer than in controls [7/1,546 vs. 1/2,012; P = 0.025; odds ratio, 9.36 (95% CI, 1.15-76.02)]. Overall, 16 (1%) of 1,579 pancreatic cancer cases had an ER stress-inducing CPA1 or CPB1 variant, compared with 1 of 2,068 controls (P < 0.00001). No other candidate genes had statistically significant differences in variant prevalence between cases and controls. Our study indicates ER stress-inducing variants in CPB1 and CPA1 are associated with pancreatic cancer susceptibility and implicate ER stress in pancreatic acinar cells in pancreatic cancer development.
ESTHER : Tamura_2018_Proc.Natl.Acad.Sci.U.S.A_115_4767
PubMedSearch : Tamura_2018_Proc.Natl.Acad.Sci.U.S.A_115_4767
PubMedID: 29669919

Title : Analysis of the bread wheat genome using whole-genome shotgun sequencing - Brenchley_2012_Nature_491_705
Author(s) : Brenchley R , Spannagl M , Pfeifer M , Barker GL , D'Amore R , Allen AM , McKenzie N , Kramer M , Kerhornou A , Bolser D , Kay S , Waite D , Trick M , Bancroft I , Gu Y , Huo N , Luo MC , Sehgal S , Gill B , Kianian S , Anderson O , Kersey P , Dvorak J , McCombie WR , Hall A , Mayer KF , Edwards KJ , Bevan MW , Hall N
Ref : Nature , 491 :705 , 2012
Abstract : Bread wheat (Triticum aestivum) is a globally important crop, accounting for 20 per cent of the calories consumed by humans. Major efforts are underway worldwide to increase wheat production by extending genetic diversity and analysing key traits, and genomic resources can accelerate progress. But so far the very large size and polyploid complexity of the bread wheat genome have been substantial barriers to genome analysis. Here we report the sequencing of its large, 17-gigabase-pair, hexaploid genome using 454 pyrosequencing, and comparison of this with the sequences of diploid ancestral and progenitor genomes. We identified between 94,000 and 96,000 genes, and assigned two-thirds to the three component genomes (A, B and D) of hexaploid wheat. High-resolution synteny maps identified many small disruptions to conserved gene order. We show that the hexaploid genome is highly dynamic, with significant loss of gene family members on polyploidization and domestication, and an abundance of gene fragments. Several classes of genes involved in energy harvesting, metabolism and growth are among expanded gene families that could be associated with crop productivity. Our analyses, coupled with the identification of extensive genetic variation, provide a resource for accelerating gene discovery and improving this major crop.
ESTHER : Brenchley_2012_Nature_491_705
PubMedSearch : Brenchley_2012_Nature_491_705
PubMedID: 23192148
Gene_locus related to this paper: aegta-r7w1w2 , wheat-w5asu5 , wheat-w5caq3 , wheat-w5a8u5 , wheat-a0a080yuw6 , wheat-w5d1z6 , wheat-w5d232 , wheat-w5fha9 , wheat-w5d425 , wheat-w5bnf5 , wheat-w5dsp5 , wheat-w5ia79 , wheat-w5f9d9 , wheat-w4zq98 , wheat-w5cae4 , aegta-r7w4e1 , wheat-w5gam9 , wheat-w5h0x4 , wheat-w5bda5 , wheat-w5cqa5 , wheat-w5ggq1 , wheat-w5h2c8 , wheat-w5f1j8 , wheat-a0a077rex4 , wheat-a0a1d5vkr8 , wheat-a0a1d5wx81 , wheat-a0a1d5zjt9 , wheat-a0a1d6adr6 , wheat-a0a1d6axb7 , wheat-a0a1d6s980 , wheat-a0a1d6sag1

Title : Enantioselective transesterification by Candida antarctica Lipase B immobilized on fumed silica - Kramer_2010_J.Biotechnol_150_80
Author(s) : Kramer M , Cruz JC , Pfromm PH , Rezac ME , Czermak P
Ref : J Biotechnol , 150 :80 , 2010
Abstract : Enzymatic catalysis to produce molecules such as perfumes, flavors, and fragrances has the advantage of allowing the products to be labeled "natural" for marketing in the U.S., in addition to the exquisite selectivity and stereoselectivity of enzymes that can be an advantage over chemical catalysis. Enzymatic catalysis in organic solvents is attractive if solubility issues of reactants or products, or thermodynamic issues (water as a product in esterification) complicate or prevent aqueous enzymatic catalysis. Immobilization of the enzyme on a solid support can address the generally poor solubility of enzymes in most solvents. We have recently reported on a novel immobilization method for Candida antarctica Lipase B on fumed silica to improve the enzymatic activity in hexane. This research is extended here to study the enantioselective transesterification of (RS)-1-phenylethanol with vinyl acetate. The maximum catalytic activity for this preparation exceeded the activity (on an equal enzyme amount basis) of the commercial Novozyme 435((R)) significantly. The steady-state conversion for (R)-1-phenylethanol was about 75% as confirmed via forward and reverse reaction. The catalytic activity steeply increases with increasing nominal surface coverage of the support until a maximum is reached at a nominal surface coverage of 230%. We hypothesize that the physical state of the enzyme molecules at a low surface coverage is dominated in this case by detrimental strong enzyme-substrate interactions. Enzyme-enzyme interactions may stabilize the active form of the enzyme as surface coverage increases while diffusion limitations reduce the apparent catalytic performance again at multi-layer coverage. The temperature-, solvent-, and long-term stability for CALB/fumed silica preparations showed that these preparations can tolerate temperatures up to 70 degrees C, continuous exposure to solvents, and long-term storage.
ESTHER : Kramer_2010_J.Biotechnol_150_80
PubMedSearch : Kramer_2010_J.Biotechnol_150_80
PubMedID: 20670664

Title : The B73 maize genome: complexity, diversity, and dynamics - Schnable_2009_Science_326_1112
Author(s) : Schnable PS , Ware D , Fulton RS , Stein JC , Wei F , Pasternak S , Liang C , Zhang J , Fulton L , Graves TA , Minx P , Reily AD , Courtney L , Kruchowski SS , Tomlinson C , Strong C , Delehaunty K , Fronick C , Courtney B , Rock SM , Belter E , Du F , Kim K , Abbott RM , Cotton M , Levy A , Marchetto P , Ochoa K , Jackson SM , Gillam B , Chen W , Yan L , Higginbotham J , Cardenas M , Waligorski J , Applebaum E , Phelps L , Falcone J , Kanchi K , Thane T , Scimone A , Thane N , Henke J , Wang T , Ruppert J , Shah N , Rotter K , Hodges J , Ingenthron E , Cordes M , Kohlberg S , Sgro J , Delgado B , Mead K , Chinwalla A , Leonard S , Crouse K , Collura K , Kudrna D , Currie J , He R , Angelova A , Rajasekar S , Mueller T , Lomeli R , Scara G , Ko A , Delaney K , Wissotski M , Lopez G , Campos D , Braidotti M , Ashley E , Golser W , Kim H , Lee S , Lin J , Dujmic Z , Kim W , Talag J , Zuccolo A , Fan C , Sebastian A , Kramer M , Spiegel L , Nascimento L , Zutavern T , Miller B , Ambroise C , Muller S , Spooner W , Narechania A , Ren L , Wei S , Kumari S , Faga B , Levy MJ , McMahan L , Van Buren P , Vaughn MW , Ying K , Yeh CT , Emrich SJ , Jia Y , Kalyanaraman A , Hsia AP , Barbazuk WB , Baucom RS , Brutnell TP , Carpita NC , Chaparro C , Chia JM , Deragon JM , Estill JC , Fu Y , Jeddeloh JA , Han Y , Lee H , Li P , Lisch DR , Liu S , Liu Z , Nagel DH , McCann MC , SanMiguel P , Myers AM , Nettleton D , Nguyen J , Penning BW , Ponnala L , Schneider KL , Schwartz DC , Sharma A , Soderlund C , Springer NM , Sun Q , Wang H , Waterman M , Westerman R , Wolfgruber TK , Yang L , Yu Y , Zhang L , Zhou S , Zhu Q , Bennetzen JL , Dawe RK , Jiang J , Jiang N , Presting GG , Wessler SR , Aluru S , Martienssen RA , Clifton SW , McCombie WR , Wing RA , Wilson RK
Ref : Science , 326 :1112 , 2009
Abstract : We report an improved draft nucleotide sequence of the 2.3-gigabase genome of maize, an important crop plant and model for biological research. Over 32,000 genes were predicted, of which 99.8% were placed on reference chromosomes. Nearly 85% of the genome is composed of hundreds of families of transposable elements, dispersed nonuniformly across the genome. These were responsible for the capture and amplification of numerous gene fragments and affect the composition, sizes, and positions of centromeres. We also report on the correlation of methylation-poor regions with Mu transposon insertions and recombination, and copy number variants with insertions and/or deletions, as well as how uneven gene losses between duplicated regions were involved in returning an ancient allotetraploid to a genetically diploid state. These analyses inform and set the stage for further investigations to improve our understanding of the domestication and agricultural improvements of maize.
ESTHER : Schnable_2009_Science_326_1112
PubMedSearch : Schnable_2009_Science_326_1112
PubMedID: 19965430
Gene_locus related to this paper: maize-b4ffc7 , maize-b6u7e1 , maize-c0pcy5 , maize-c0pgf7 , maize-c0pgw1 , maize-c0pfl3 , maize-b4fpr7 , maize-k7vy73 , maize-a0a096swr3 , maize-k7v3i9 , maize-b6u9v9 , maize-a0a3l6e780 , maize-b4fv80 , maize-a0a1d6nse2 , maize-c4j9a1 , maize-k7uba1

Title : Fluid balance modelling in patients with kidney failure - Chamney_1999_J.Med.Eng.Technol_23_45
Author(s) : Chamney PW , Johner C , Aldridge C , Kramer M , Valasco N , Tattersall JE , Aukaidey T , Gordon R , Greenwood RN
Ref : J Med Eng Technol , 23 :45 , 1999
Abstract : In patients with kidney failure, adequate control of fluid status remains one of the most difficult routine issues to be addressed in the modern style of dialysis. This is primarily due to the lack of quantitative methods for the assessment of fluid status and the reliance on subjective criteria. Fluid is removed from the blood during dialysis treatments using a process called ultrafiltration. The last decade has seen considerable developments in blood volume monitoring (BVM) technology which has enabled responses to ultrafiltration to be continually monitored on an individual basis. This has enabled feedback control of patients' blood volume to be applied with partial success, reducing the number of symptoms. The feedback control algorithms employed have been relatively unsophisticated, using simple proportional control with no attempt to include models of the patient fluid dynamics. This paper describes the development of some prototype fluid kinetic models which may be used in a more advanced control system. Initial results demonstrate the importance of active control processes in the patients' physiological compensatory mechanisms.
ESTHER : Chamney_1999_J.Med.Eng.Technol_23_45
PubMedSearch : Chamney_1999_J.Med.Eng.Technol_23_45
PubMedID: 10356673