Wood DW

References (3)

Title : A Novel, Modified Human Butyrylcholinesterase Catalytically Degrades the Chemical Warfare Nerve Agent, Sarin - McGarry_2020_Toxicol.Sci_174_133
Author(s) : McGarry KG , Lalisse RF , Moyer RA , Johnson KM , Tallan AM , Winters TP , Taris JE , McElroy CA , Lemmon EE , Shafaat HS , Fan Y , Deal A , Marguet SC , Harvilchuck JA , Hadad CM , Wood DW
Ref : Toxicol Sci , 174 :133 , 2020
Abstract : Chemical warfare nerve agents (CWNAs) present a global threat to both military and civilian populations. The acute toxicity of CWNAs stems from their ability to effectively inhibit acetylcholinesterase (AChE). This inhibition can lead to uncontrolled cholinergic cellular signaling, resulting in cholinergic crisis and, ultimately, death. While the current FDA-approved standard of care is moderately effective when administered early, development of novel treatment strategies is necessary. Butyrylcholinesterase (BChE) is an enzyme which displays a high degree of structural homology to AChE. Unlike AChE, the roles of BChE are uncertain and possibilities are still being explored. However, BChE appears to primarily serve as a bioscavenger of toxic esters due to its ability to accommodate a wide variety of substrates within its active site. Like AChE, BChE is also readily inhibited by CWNAs. Due to its high affinity for binding CWNAs, and that null-BChE yields no apparent health effects, exogenous BChE has been explored as a candidate therapeutic for CWNA intoxication. Despite years of research, minimal strides have been made to develop a catalytic bioscavenger. Further, BChE is only in early clinical trials as a stoichiometric bioscavenger of CWNAs, and large quantities must be administered to treat CWNA toxicity. Here we describe previously unidentified mutations to residues within and adjacent to the acyl binding pocket (positions 282-285 were mutagenized from YGTP to NHML) of BChE that confer catalytic degradation of the CWNA, sarin. These mutations, along with corresponding future efforts, may finally lead to a novel therapeutic to combat CWNA intoxication.
ESTHER : McGarry_2020_Toxicol.Sci_174_133
PubMedSearch : McGarry_2020_Toxicol.Sci_174_133
PubMedID: 31879758

Title : Genome sequences of three agrobacterium biovars help elucidate the evolution of multichromosome genomes in bacteria - Slater_2009_J.Bacteriol_191_2501
Author(s) : Slater SC , Goldman BS , Goodner B , Setubal JC , Farrand SK , Nester EW , Burr TJ , Banta L , Dickerman AW , Paulsen I , Otten L , Suen G , Welch R , Almeida NF , Arnold F , Burton OT , Du Z , Ewing A , Godsy E , Heisel S , Houmiel KL , Jhaveri J , Lu J , Miller NM , Norton S , Chen Q , Phoolcharoen W , Ohlin V , Ondrusek D , Pride N , Stricklin SL , Sun J , Wheeler C , Wilson L , Zhu H , Wood DW
Ref : J. Bacteriol , 191 :2501 , 2009
Abstract : The family Rhizobiaceae contains plant-associated bacteria with critical roles in ecology and agriculture. Within this family, many Rhizobium and Sinorhizobium strains are nitrogen-fixing plant mutualists, while many strains designated as Agrobacterium are plant pathogens. These contrasting lifestyles are primarily dependent on the transmissible plasmids each strain harbors. Members of the Rhizobiaceae also have diverse genome architectures that include single chromosomes, multiple chromosomes, and plasmids of various sizes. Agrobacterium strains have been divided into three biovars, based on physiological and biochemical properties. The genome of a biovar I strain, A. tumefaciens C58, has been previously sequenced. In this study, the genomes of the biovar II strain A. radiobacter K84, a commercially available biological control strain that inhibits certain pathogenic agrobacteria, and the biovar III strain A. vitis S4, a narrow-host-range strain that infects grapes and invokes a hypersensitive response on nonhost plants, were fully sequenced and annotated. Comparison with other sequenced members of the Alphaproteobacteria provides new data on the evolution of multipartite bacterial genomes. Primary chromosomes show extensive conservation of both gene content and order. In contrast, secondary chromosomes share smaller percentages of genes, and conserved gene order is restricted to short blocks. We propose that secondary chromosomes originated from an ancestral plasmid to which genes have been transferred from a progenitor primary chromosome. Similar patterns are observed in select Beta- and Gammaproteobacteria species. Together, these results define the evolution of chromosome architecture and gene content among the Rhizobiaceae and support a generalized mechanism for second-chromosome formation among bacteria.
ESTHER : Slater_2009_J.Bacteriol_191_2501
PubMedSearch : Slater_2009_J.Bacteriol_191_2501
PubMedID: 19251847
Gene_locus related to this paper: agrrk-b9j7k2 , agrrk-b9j8e4 , agrrk-b9j8g5 , agrrk-b9j9n4 , agrrk-b9j9p4 , agrrk-b9ja88 , agrrk-b9jbs5 , agrrk-b9jd67 , agrrk-b9jd85 , agrrk-b9jfh5 , agrrk-b9jfj6 , agrrk-b9jfu6 , agrrk-b9jfy6 , agrrk-b9jh78 , agrrk-b9ji04 , agrrk-b9jih5 , agrrk-b9jih7 , agrrk-b9jj14 , agrrk-b9jjt5 , agrrk-b9jjt6 , agrrk-b9jk42 , agrrk-b9jki6 , agrrk-b9jkt4 , agrrk-b9jla0 , agrrk-b9jlc3 , agrrk-b9jlj1 , agrrk-b9jlj2 , agrrk-b9jlr1 , agrrk-b9jmj9 , agrrk-b9jml0 , agrrk-b9jmn1 , agrrk-b9jnw6 , agrrk-b9jq01 , agrrk-b9jq11 , agrrk-b9jq35 , agrtu-DHAA , agrvs-b9jqv2 , agrvs-b9jr09 , agrvs-b9js24 , agrvs-b9js61 , agrvs-b9ju03 , agrvs-b9jw40 , agrvs-b9jx20 , agrvs-b9jy84 , agrvs-b9k1h8 , agrvs-b9k2m9 , agrvs-b9k3r6 , agrvs-b9k5p9 , agrvs-b9k093 , agrvs-b9k188 , agrvs-b9k312 , agrrk-b9jls9 , agrrk-b9jca1 , agrvs-b9jur1 , agrrk-rutd

Title : The genome of the natural genetic engineer Agrobacterium tumefaciens C58 - Wood_2001_Science_294_2317
Author(s) : Wood DW , Setubal JC , Kaul R , Monks DE , Kitajima JP , Okura VK , Zhou Y , Chen L , Wood GE , Almeida NF, Jr. , Woo L , Chen Y , Paulsen IT , Eisen JA , Karp PD , Bovee D, Sr. , Chapman P , Clendenning J , Deatherage G , Gillet W , Grant C , Kutyavin T , Levy R , Li MJ , McClelland E , Palmieri A , Raymond C , Rouse G , Saenphimmachak C , Wu Z , Romero P , Gordon D , Zhang S , Yoo H , Tao Y , Biddle P , Jung M , Krespan W , Perry M , Gordon-Kamm B , Liao L , Kim S , Hendrick C , Zhao ZY , Dolan M , Chumley F , Tingey SV , Tomb JF , Gordon MP , Olson MV , Nester EW
Ref : Science , 294 :2317 , 2001
Abstract : The 5.67-megabase genome of the plant pathogen Agrobacterium tumefaciens C58 consists of a circular chromosome, a linear chromosome, and two plasmids. Extensive orthology and nucleotide colinearity between the genomes of A. tumefaciens and the plant symbiont Sinorhizobium meliloti suggest a recent evolutionary divergence. Their similarities include metabolic, transport, and regulatory systems that promote survival in the highly competitive rhizosphere; differences are apparent in their genome structure and virulence gene complement. Availability of the A. tumefaciens sequence will facilitate investigations into the molecular basis of pathogenesis and the evolutionary divergence of pathogenic and symbiotic lifestyles.
ESTHER : Wood_2001_Science_294_2317
PubMedSearch : Wood_2001_Science_294_2317
PubMedID: 11743193
Gene_locus related to this paper: agrt5-a9cf94 , agrt5-a9cfa9 , agrt5-a9cfs8 , agrt5-a9cfu7 , agrt5-a9cie7 , agrt5-a9cj11 , agrt5-a9cjp2 , agrt5-a9cki2 , agrt5-a9ckr2 , agrt5-a9ckt2 , agrt5-a9cle4 , agrt5-a9clq8 , agrt5-a9clq9 , agrt5-q7cx24 , agrt5-q7d1j0 , agrt5-q7d1j3 , agrt5-q7d3m5 , agrt5-y5261 , agrtu-ACVB , agrtu-ATTS , agrtu-ATU0253 , agrtu-ATU0403 , agrtu-ATU0841 , agrtu-ATU1045 , agrtu-ATU1102 , agrtu-ATU1572 , agrtu-ATU1617 , agrtu-ATU1826 , agrtu-ATU1842 , agrtu-ATU2061 , agrtu-ATU2126 , agrtu-ATU2171 , agrtu-ATU2409 , agrtu-ATU2452 , agrtu-ATU2481 , agrtu-ATU2497 , agrtu-ATU2576 , agrtu-ATU3428 , agrtu-ATU3651 , agrtu-ATU3652 , agrtu-ATU4238 , agrtu-ATU5190 , agrtu-ATU5193 , agrtu-ATU5275 , agrtu-ATU5296 , agrtu-ATU5348 , agrtu-ATU5389 , agrtu-ATU5446 , agrtu-ATU5495 , agrtu-CPO , agrtu-DHAA , agrtu-DLHH , agrtu-EPHA , agrtu-GRST , agrtu-PCA , agrtu-PCAD , agrtu-PHBC , agrtu-PTRB , agrt5-a9cji8