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arcfu-est2

Archaeoglobus fulgidus thermophilic esterase AF1537

Comment

A thermophilic esterase with high activity towards short- to medium-chain p-nitrophenyl carboxylic esters with optimal activity towards the valerate ester. The AF-Est2 has good solvent and pH stability and is very thermostable, showing no loss of activity after incubation for 30min at 80 C. The structure reveals a bound CoA molecule in the vicinity of the active site which regulates substrate specificity

Relationship

Family : 6_AlphaBeta_hydrolase

Block : X

Position in NCBI Life Tree : Archaeoglobus fulgidus

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Archaea N E > Euryarchaeota N E > Archaeoglobi N E > Archaeoglobales N E > Archaeoglobaceae N E > Archaeoglobus N E > Archaeoglobus fulgidus N E

Molecular evidence

No mutation

No kinetic

No disease

No inhibitor

Database

Sequence

Peptide

MLERVFIDVD GVKVSLLKGR ERKVFYIHSS GSDATQWVNQ LTAIGGYAID LPNHGQSDTV EVNSVDEYAY YASESLKKTV GKAVVVGHSL GGAVAQKLYL RNPEICLALV LVGTGARLRV LPEILEGLKK EPEKAVDLML SMAFASKGEE YEKKRREFLD RVDVLHLDLS LCDRFDLLED YRNGKLKIGV PTLVIVGEED KLTPLKYHEF FHKHIPNSEL VVIPGASHMV MLEKHVEFNE ALEKFLKKVG V

References (2)

Title : Structural and biochemical characterisation of Archaeoglobus fulgidus esterase reveals a bound CoA molecule in the vicinity of the active site - Sayer_2016_Sci.Rep_6_25542
Author(s) : Sayer C , Finnigan W , Isupov MN , Levisson M , Kengen SW , Van der Oost J , Harmer NJ , Littlechild JA
Ref : Sci Rep , 6 :25542 , 2016
Abstract :
PubMedSearch : Sayer_2016_Sci.Rep_6_25542
PubMedID: 27160974
Gene_locus related to this paper: arcfu-est2

Title : The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus - Klenk_1997_Nature_390_364
Author(s) : Klenk HP , Clayton RA , Tomb JF , White O , Nelson KE , Ketchum KA , Dodson RJ , Gwinn M , Hickey EK , Peterson JD , Richardson DL , Kerlavage AR , Graham DE , Kyrpides NC , Fleischmann RD , Quackenbush J , Lee NH , Sutton GG , Gill S , Kirkness EF , Dougherty BA , McKenney K , Adams MD , Loftus B , Peterson S , Reich CI , McNeil LK , Badger JH , Glodek A , Zhou L , Overbeek R , Gocayne JD , Weidman JF , McDonald L , Utterback T , Cotton MD , Spriggs T , Artiach P , Kaine BP , Sykes SM , Sadow PW , D'Andrea KP , Bowman C , Fujii C , Garland SA , Mason TM , Olsen GJ , Fraser CM , Smith HO , Woese CR , Venter JC
Ref : Nature , 390 :364 , 1997
Abstract :
PubMedSearch : Klenk_1997_Nature_390_364
PubMedID: 9389475
Gene_locus related to this paper: arcfu-AF0514 , arcfu-AF0675 , arcfu-AF1134 , arcfu-AF1563 , arcfu-AF1753 , arcfu-AF1763 , arcfu-est1 , arcfu-est2 , arcfu-est3 , arcfu-estea , arcfu-o28594 , arcfu-o29442 , arcfu-pcbd