luccu-E3aest7

Lucilia cuprina (and Lucilia sericata (Green bottle fly) (Phaenicia sericata)) alpha cluster carboxylesterase (OP resistance (LcaE7) E3

Comment

Relationship

Family : Carb_B_Arthropoda

Block : C

Position in NCBI Life Tree : Lucilia cuprina

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Eukaryota N E > Opisthokonta N E > Metazoa N E > Eumetazoa N E > Bilateria N E > Protostomia N E > Ecdysozoa N E > Panarthropoda N E > Arthropoda N E > Mandibulata N E > Pancrustacea N E > Hexapoda N E > Insecta N E > Dicondylia N E > Pterygota N E > Neoptera N E > Holometabola N E > Diptera N E > Brachycera N E > Muscomorpha N E > Eremoneura N E > Cyclorrhapha N E > Schizophora N E > Calyptratae N E > Oestroidea N E > Calliphoridae N E > Luciliinae N E > Lucilia N E > Lucilia cuprina N E

Molecular evidence

No mutation

5C8V - Lucilia cuprina alpha esterase 7: Gly137Asp 4UBI - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 3.70 MGy at 100K 4UBJ - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 5.55 MGy at 100K 4UBK - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 7.40 MGy at 100K 4UBL - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 9.26 MGy 4UBM - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 11.11 MGy at 100K 4UBN - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 1.85 MGy at 150K 4UBO - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 3.70 MGy at 150K 4W1P - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 5.54 MGy at 150K 4W1Q - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 7.39 MGy at 150K 4W1R - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 9.24 MGy at 150K 4W1S - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 11.09 MGy at 150K 4QWM - Kinetic crystallography of alphaE7-carboxylesterse from Lucilia cuprina.Absorbed X-ray dose 1.85 MGy 5TYJ - alpha-esterase-7 in complex with (3-bromo-5-phenoxylphenyl)boronic acid 5TYK - alpha-esterase-7 in complex with 3-chloro-4-[(2-fluorophenyl)methoxy]phenylboronic acid 5TYL - alpha-esterase-7 in complex with naphthalen-2-ylboronic acid 5TYM - alpha-esterase-7 in complex with [3-bromo-5-(pyrrolidin-1-yl)phenyl]borinic acid 5TYN - alpha-esterase-7 in complex with [3-bromo-5-(pyrrolidin-1-yl)phenyl]borinic acid 5TYO - alpha-esterase-7 in complex with [3-(benzyloxy)-4-methylphenyl]borinic acid 5TYP - alpha-esterase-7 in complex with (3-bromo-4-methylphenyl)boronic acid 4FNM - The alpha-esterase-7 carboxylesterase, E3, from the blowfly Lucilia cuprina 1 DEUP phosphorylated (DEUP inhibited diethyl phosphate adduct) 4FNG - The alpha-esterase-7 carboxylesterase, E3, from the blowfly Lucilia cuprina 2 5CH3 - The alpha-esterase-7 carboxylesterase, E3, from the blowfly Lucilia cuprina: re-refinement Apo 5CH5 - The alpha-esterase-7 carboxylesterase, E3, from the blowfly Lucilia cuprina: re-refinement DEUP phosphorylated (DEUP inhibited diethyl phosphate adduct) 5IVK - The alpha-esterase-7 carboxylesterase, E3, from the blowfly Lucilia cuprina: phosphorylated-enzyme ensemble refinement (DEUP inhibited diethyl phosphate adduct) 5IVD - The alpha-esterase-7 carboxylesterase, E3, from the blowfly Lucilia cuprina: apo-enzyme qFit multi-conformer model 5IVH - The alpha-esterase-7 carboxylesterase, E3, from the blowfly Lucilia cuprina: apo-enzyme ensemble refinement 5IVI - The alpha-esterase-7 carboxylesterase, E3, from the blowfly Lucilia cuprina: apo-enzyme qFit multi-conformer model (DEUP inhibited diethyl phosphate adduct) 5IKX - Crystal structure of the alpha-esterase-7 carboxylesterase, E3, from Lucilia cuprina (supersedes 4FG5 06-Jun-2013)

No kinetic

No disease

Sequence

Peptide

MNFNVSLMEK LKWKIKCIEN KFLNYRLTTN ETVVAETEYG KVKGVKRLTV YDDSYYSFEG IPYAQPPVGE LRFKAPQRPT PWDGVRDCCN HKDKSVQVDF ITGKVCGSED CLYLSVYTNN LNPETKRPVL VYIHGGGFII GENHRDMYGP DYFIKKDVVL INIQYRLGAL GFLSLNSEDL NVPGNAGLKD QVMALRWIKN NCANFGGNPD NITVFGESAG AASTHYMMLT EQTRGLFHRG ILMSGNAICP WANTQCQHRA FTLAKLAGYK GEDNDKDVLE FLMKAKPQDL IKLEEKVLTL EERTNKVMFP FGPTVEPYQT ADCVLPKHPR EMVKTAWGNS IPTMMGNTSY EGLFFTSILK QMPMLVKELE TCVNFVPSEL ADAERTAPET LEMGAKIKKA HVTGETPTAD NFMDLCSHIY FWFPMHRLLQ LRFNHTSGTP VYLYRFDFDS EDLINPYRIM RSGRGVKGVS HADELTYFFW NQLAKRMPKE SREYKTIERM TGIWIQFATT GNPYSNEIEG MENVSWDPIK KSDEVYKCLN ISDELKMIDV PEMDKIKQWE SMFEKHRDLF

References (15)

Title : Overcoming insecticide resistance through computational inhibitor design - Correy_2019_Proc.Natl.Acad.Sci.U.S.A_116_21012
Author(s) : Correy GJ , Zaidman D , Harmelin A , Carvalho S , Mabbitt PD , Calaora V , James PJ , Kotze AC , Jackson CJ , London N
Ref : Proc Natl Acad Sci U S A , 116 :21012 , 2019
PubMedID: 31575743
Gene_locus related to this paper: luccu-E3aest7

Title : Evolution of Protein Quaternary Structure in Response to Selective Pressure for Increased Thermostability - Fraser_2016_J.Mol.Biol_428_2359
Author(s) : Fraser NJ , Liu JW , Mabbitt PD , Correy GJ , Coppin CW , Lethier M , Perugini MA , Murphy JM , Oakeshott JG , Weik M , Jackson CJ
Ref : Journal of Molecular Biology , 428 :2359 , 2016
PubMedID: 27016206
Gene_locus related to this paper: luccu-E3aest7

Title : Mapping the Accessible Conformational Landscape of an Insect Carboxylesterase Using Conformational Ensemble Analysis and Kinetic Crystallography - Correy_2016_Structure_24_977
Author(s) : Correy GJ , Carr PD , Meirelles T , Mabbitt PD , Fraser NJ , Weik M , Jackson CJ
Ref : Structure , 24 :977 , 2016
PubMedID: 27210287
Gene_locus related to this paper: luccu-E3aest7

Title : Conformational Disorganization within the Active Site of a Recently Evolved Organophosphate Hydrolase Limits Its Catalytic Efficiency - Mabbitt_2016_Biochemistry_55_1408
Author(s) : Mabbitt PD , Correy GJ , Meirelles T , Fraser NJ , Coote ML , Jackson CJ
Ref : Biochemistry , 55 :1408 , 2016
PubMedID: 26881849
Gene_locus related to this paper: luccu-E3aest7

Title : Structure and function of an insect alpha-carboxylesterase (alphaEsterase7) associated with insecticide resistance - Jackson_2013_Proc.Natl.Acad.Sci.U.S.A_110_10177
Author(s) : Jackson CJ , Liu JW , Carr PD , Younus F , Coppin C , Meirelles T , Lethier M , Pandey G , Ollis DL , Russell RJ , Weik M , Oakeshott JG
Ref : Proc Natl Acad Sci U S A , 110 :10177 , 2013
PubMedID: 23733941
Gene_locus related to this paper: luccu-E3aest7

Title : Testing the evolvability of an insect carboxylesterase for the detoxification of synthetic pyrethroid insecticides - Coppin_2012_Insect.Biochem.Mol.Biol_42_343
Author(s) : Coppin CW , Jackson CJ , Sutherland T , Hart PJ , Devonshire AL , Russell RJ , Oakeshott JG
Ref : Insect Biochemistry & Molecular Biology , 42 :343 , 2012
PubMedID: 22300675
Gene_locus related to this paper: luccu-E3aest7

Title : Hydrolysis of individual isomers of fluorogenic pyrethroid analogs by mutant carboxylesterases from Lucilia cuprina - Devonshire_2007_Insect.Biochem.Mol.Biol_37_891
Author(s) : Devonshire AL , Heidari R , Huang HZ , Hammock BD , Russell RJ , Oakeshott JG
Ref : Insect Biochemistry & Molecular Biology , 37 :891 , 2007
PubMedID: 17681228
Gene_locus related to this paper: luccu-E3aest7

Title : Hydrolysis of pyrethroids by carboxylesterases from Lucilia cuprina and Drosophila melanogaster with active sites modified by in vitro mutagenesis - Heidari_2005_Insect.Biochem.Mol.Biol_35_597
Author(s) : Heidari R , Devonshire AL , Campbell BE , Dorrian SJ , Oakeshott JG , Russell RJ
Ref : Insect Biochemistry & Molecular Biology , 35 :597 , 2005
PubMedID: 15857765
Gene_locus related to this paper: drome-EST23aes07 , luccu-E3aest7

Title : Multiple mutations and gene duplications conferring organophosphorus insecticide resistance have been selected at the Rop-1 locus of the sheep blowfly, Lucilia cuprina - Newcomb_2005_J.Mol.Evol_60_207
Author(s) : Newcomb RD , Gleeson DM , Yong CG , Russell RJ , Oakeshott JG
Ref : Journal of Molecular Evolution , 60 :207 , 2005
PubMedID: 15785849
Gene_locus related to this paper: luccu-E3aest7

Title : Hydrolysis of organophosphorus insecticides by in vitro modified carboxylesterase E3 from Lucilia cuprina - Heidari_2004_Insect.Biochem.Mol.Biol_34_353
Author(s) : Heidari R , Devonshire AL , Campbell BE , Bell KL , Dorrian SJ , Oakeshott JG , Russell RJ
Ref : Insect Biochemistry & Molecular Biology , 34 :353 , 2004
PubMedID: 15041019
Gene_locus related to this paper: luccu-E3aest7

Title : Kinetic efficiency of mutant carboxylesterases implicated in organophosphate insecticide resistance - Devonshire_2003_Pestic.Biochem.Physiol_76_1
Author(s) : Devonshire AL , Heidari R , Bell KL , Campbell PM , Campbell BE , Odgers WA , Oakeshott JG , Russell RJ
Ref : Pesticide Biochemistry and Physiology , 76 :1 , 2003
PubMedID:
Gene_locus related to this paper: luccu-E3aest7 , musdo-EST23aes07

Title : Two different amino acid substitutions in the ali-esterase, E3, confer alternative types of organophosphorus insecticide resistance in the sheep blowfly, Lucilia cuprina - Campbell_1998_Insect.Biochem.Mol.Biol_28_139
Author(s) : Campbell PM , Newcomb RD , Russell RJ , Oakeshott JG
Ref : Insect Biochemistry & Molecular Biology , 28 :139 , 1998
PubMedID:
Gene_locus related to this paper: luccu-E3aest7

Title : A single amino acid substitution converts a carboxylesterase to an organophosphorus hydrolase and confers insecticide resistance on a blowfly - Newcomb_1997_Proc.Natl.Acad.Sci.U.S.A_94_7464
Author(s) : Newcomb RD , Campbell PM , Ollis DL , Cheah E , Russell RJ , Oakeshott JG
Ref : Proceedings of the National Academy of Sciences of the United States of America , 94 :7464 , 1997
PubMedID: 9207114
Gene_locus related to this paper: luccu-ACHE , luccu-E3aest7

Title : Biochemistry of esterases associated with organophosphate resistance in Lucilia cuprina with comparisons to putative orthologues in other Diptera - Campbell_1997_Biochem.Genet_35_17
Author(s) : Campbell PM , Trott JF , Claudianos C , Smyth KA , Russell RJ , Oakeshott JG
Ref : Biochemical Genetics , 35 :17 , 1997
PubMedID: 9238516
Gene_locus related to this paper: luccu-E3aest7

Title : Isolation of alpha cluster esterase genes associated with organophosphate resistance in Lucilia cuprina - Newcomb_1996_Insect.Mol.Biol_5_211
Author(s) : Newcomb RD , East PD , Russell RJ , Oakeshott JG
Ref : Insect Molecular Biology , 5 :211 , 1996
PubMedID: 8799740
Gene_locus related to this paper: luccu-E3aest7