HaloTag-ligand

General

The HaloTag is composed of covalently bound haloalkane dehalogenase and a synthetic ligand of choice. The bacterial enzyme can be fused to various proteins of interest to visualize its subcellular localization.Ssynthetic ligands consist of a reactive chloroalkane linker bound to a functional group such as biotin (can be used as an affinity tag) or a fluorescent dye (Coumarin, Oregon Green, Alexa Fluor 488, diAcFAM, or TMR)
Inhibitor Main targeted enzyme families Type Structure
Chloroalkane-Cyanine3-fluorophore Haloalkane_dehalogenase-HLD2 Indole HaloTag-ligand
JF669-HaloTag Haloalkane_dehalogenase-HLD2 HaloTag-ligand Azetidin Benzosilin
TMR-S4 Haloalkane_dehalogenase-HLD2 HaloTag-ligand Sulfur Compound Sulfonamide Xanthene
TMR-T4 Haloalkane_dehalogenase-HLD2 HaloTag-ligand Sulfur Compound Sulfonamide Xanthene
TMR-S5 Haloalkane_dehalogenase-HLD2 HaloTag-ligand Sulfur Compound Sulfonamide Xanthene
TMR-Hy5 Haloalkane_dehalogenase-HLD2 HaloTag-ligand Xanthene
TMR-T5 Haloalkane_dehalogenase-HLD2 HaloTag-ligand Sulfur Compound Sulfonamide Xanthene
TRaQ-G-ILQ Haloalkane_dehalogenase-HLD2 HaloTag-ligand Indole Benzosilin
TRaQ-G-ILU Haloalkane_dehalogenase-HLD2 HaloTag-ligand Benzosilin
TRaQ-G-ctrl Haloalkane_dehalogenase-HLD2 HaloTag-ligand Benzosilin
Me-TRaQ-G Haloalkane_dehalogenase-HLD2 HaloTag-ligand Benzosilin
VL1-8MH Haloalkane_dehalogenase-HLD2 Fluorescent Probe HaloTag-ligand Isoquinoline Quinoline
UL2-8MS Haloalkane_dehalogenase-HLD2 Fluorescent Probe HaloTag-ligand
Chloroalkane-carbopyronine-fluorophore Haloalkane_dehalogenase-HLD2 Fluorescent Probe HaloTag-ligand
Chloroalkane-tetramethylrhodamine-fluorophore Haloalkane_dehalogenase-HLD2 Fluorescent Probe HaloTag-ligand Rhodamine derivative Xanthene
tetramethylrhodamine-HaloTag-ligand Haloalkane_dehalogenase-HLD2 Fluorescent Probe HaloTag-ligand Rhodamine derivative Xanthene
JF635i Haloalkane_dehalogenase-HLD2 Fluorescent Probe HaloTag-ligand
JF635 Haloalkane_dehalogenase-HLD2 Fluorescent Probe HaloTag-ligand Azetidin Carboxamide Benzofuran Benzosilin
dansyl-PEG2-HaloTag Haloalkane_dehalogenase-HLD2 Polymer Poly-ethylene-glycol Sulfonamide Sulfur Compound HaloTag-ligand Naphthalen
HALSTS-1Q9 Haloalkane_dehalogenase-HLD2 HaloTag-ligand Tetrazol
HALSTS1 Haloalkane_dehalogenase-HLD2 HaloTag-ligand Tetrazol