PlaB
Relationship
Comment
Bacterial phospholipases. Legionella pneumophila possesses a major cell-associated hemolytic phospholipase A (PlaB) which shares no homology to described phospholipases. PlaB preferentially hydrolyzes long-chain fatty acid substrates containing 12 or more carbon atoms. PlaB shows concentration-dependent phospholipase inactivation by tetramerization which may be a mechanism for self-protection. See Papers: Bender et al. 2009 and Kuhle et al. 2014. The tetramer is a dimer of identical dimers. Diwo et al. found in the structure eight NAD(H) molecules at the dimer/dimerinterface, suggesting that these molecules stabilize the tetramer leading to enzyme inactivation.
Database
Interpro : No interpro
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF12697 Abhydrolase_6
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 6ZTH | Phospholipase PlaB from Legionella pneumophila | legpn-i7i328 |
| 6ZTI | Phospholipase PlaB from Legionella pneumophila in complex with thio-NAD | legpn-i7i328 |
| Gene_locus | Name | Species |
|---|---|---|
| 9alte-a6evy1 | Marinobacter algicola DG893 Uncharacterized protein | Marinobacter algicola DG893 |
| 9alte-h3zi92 | Alishewanella jeotgali, aestuarii, agri, Uncharacterized protein | Alishewanella jeotgali KCTC 22429 |
| 9bact-b5jq87 | Verrucomicrobiae bacterium DG1235 Putative uncharacterized protein | Verrucomicrobiae bacterium DG1235 |
| 9bact-m1z099 | Nitrospina gracilis 3\/211 Uncharacterized protein | Nitrospina gracilis 3\/211 |
| 9gamm-a9cwc2 | Shewanella benthica KT99 Phospholipase | Shewanella benthica KT99 |
| 9gamm-i4vhy2 | Rhodanobacter sp. 115 Phospholipase | Rhodanobacter sp. 115 |
| 9gamm-i4w119 | Rhodanobacter fulvus Jip2 Phospholipase | Rhodanobacter fulvus Jip2 |
| 9gamm-i4w709 | Rhodanobacter spathiphylli B39 Phospholipase | Rhodanobacter spathiphylli B39 |
| 9gamm-m4ni10 | Rhodanobacter denitrificans, Rhodanobacter thiooxydans, Uncharacterized protein | Rhodanobacter denitrificans |
| 9gamm-w0drv6 | Thioalkalivibrio thiocyanoxidans ARh 4 Uncharacterized protein | Thioalkalivibrio thiocyanoxidans ARh 4 |
| 9gamm-w7q5c5 | Halomonas sp. BC04 Uncharacterized protein | Halomonas sp. BC04 |
| 9prot-q0exh0 | Mariprofundus ferrooxydans PV-1 Phospholipase | Mariprofundus ferrooxydans PV-1 |
| agaal-r9psh6 | Agarivorans albus MKT 106 Phospholipase | Agarivorans albus MKT 106 |
| desac-q1jwt9 | Desulfuromonas acetoxidans DSM 684 Phospholipase | Desulfuromonas acetoxidans DSM 684 |
| flutr-f2ii77 | Fluviicola taffensis (strain DSM 16823 \/ RW262 \/ RW262) Phospholipase | Fluviicola taffensis |
| legln-d3hq45 | Legionella longbeachae Phospholipase | Legionella longbeachae NSW150 |
| legpn-i7i328 |  Legionella pneumophila subsp. pneumophila Legionella drancourtii, Phospholipase LpPlaB |
Legionella pneumophila |
| legsp-a3fmk8 | Legionella spiritensis PlaB | Legionella spiritensis |
| marmm-q0arp3 | Maricaulis maris (strain MCS10) Uncharacterized protein | Maricaulis maris |
| noss1-ALR3140 | Anabaena sp., Nostoc sp., Anabaena variabilis, hypothetical protein alr3140 | Anabaena sp. |
| permh-c0qr46 | Persephonella marina (strain DSM 14350 \/ EX-H1) Uncharacterized protein | Persephonella marina |
| phymf-i0ibh4 | Phycisphaera mikurensis (strain NBRC 102666 \/ KCTC 22515 \/ FYK2301M01) Uncharacterized protein | Phycisphaera mikurensis |
| pseae-PA2927 | Pseudomonas aeruginosa hypothetical protein pa2927 PaPlaB | Pseudomonas aeruginosa |
| psepu-n9w5v8 | Pseudomonas putida TRO1 Uncharacterized protein | Pseudomonas putida TRO1 |
| psyin-a1srl0 | Psychromonas ingrahamii (strain 37) Phospholipase | Psychromonas ingrahamii |
| sheam-a1saw0 | Shewanella amazonensis (strain ATCC BAA-1098 \/ SB2B) Phospholipase | Shewanella amazonensis |
| shehh-b0tju7 | Shewanella halifaxensis (strain HAW-EB4) Phospholipase | Shewanella halifaxensis |
| shelp-a3q9j5 | Shewanella loihica (strain ATCC BAA-1088 \/ PV-4) Phospholipase | Shewanella loihica |
| shepa-a8gza7 | Shewanella pealeana (strain ATCC 700345 \/ ANG-SQ1) Phospholipase | Shewanella pealeana |
| shevd-d4zd33 | Shewanella violacea (strain JCM 10179 \/ CIP 106290 \/ LMG 19151 \/ DSS12) Uncharacterized protein | Shewanella violacea |
| solue-q01yh7 | Solibacter usitatus (strain Ellin6076) Uncharacterized protein | Solibacter usitatus |
| Gene_locus_frgt | Name | Species |
|---|---|---|
| 9gamm-w0dn29 | Thioalkalivibrio thiocyanoxidans, Thioalkalivibrio nitratireducens, Uncharacterized protein | Thioalkalivibrio thiocyanoxidans ARh 4 |
| phopr-q6lki1 | Photobacterium profundum (Photobacterium sp. (strain SS9)) Uncharacterized protein | Photobacterium profundum |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| Dipalmitoylphosphatidicacid | [(2R)-2-hexadecanoyloxy-3-phosphonooxypropyl] hexadecanoate | legpn-i7i328 |
| Dipalmitoylphosphatidylcholine | 2,3-di(hexadecanoyloxy)propyl 2-(trimethylazaniumyl)ethyl phosphate | legpn-i7i328 human-LCAT |
| Dipalmitoylphosphatidylglycerol | [3-[2,3-dihydroxypropoxy(hydroxy)phosphoryl]oxy-2-hexadecanoyloxypropyl] hexadecanoate | legpn-i7i328 |
| Heptadecanoylphosphatidylcholine | [(2R)-3-heptadecanoyloxy-2-hydroxypropyl] 2-(trimethylazaniumyl)ethyl phosphate | pseae-PA2927 legpn-i7i328 |
| Inhibitor | Chemical Nomenclature | Proteins |
|---|---|---|
| thio-NAD | [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl][[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2R,3S,4R,5R)-5-(3-carbamothioylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate | legpn-i7i328 |
References (4)
| Title : A phospholipase B from Pseudomonas aeruginosa with activity towards endogenous phospholipids affects biofilm assembly - Weiler_2022_Biochim.Biophys.Acta.Mol.Cell.Biol.Lipids_1867_159101 |
| Author(s) : Weiler AJ , Spitz O , Gudzuhn M , Schott-Verdugo SN , Kamel M , Thiele B , Streit WR , Kedrov A , Schmitt L , Gohlk H , Kovacic F , Gohlke H |
| Ref : Biochimica & Biophysica Acta Molecular & Cellular Biology Lipids , 1867 :159101 , 2022 |
| Abstract : |
| PubMedSearch : Weiler_2022_Biochim.Biophys.Acta.Mol.Cell.Biol.Lipids_1867_159101 |
| PubMedID: 35063652 |
| Gene_locus related to this paper: pseae-PA2927 |
| Title : NAD(H)-mediated tetramerization controls the activity of Legionella pneumophila phospholipase PlaB - Diwo_2021_Proc.Natl.Acad.Sci.U.S.A_118_e2017046118 |
| Author(s) : Diwo M , Michel W , Aurass P , Kuhle-Keindorf K , Pippel J , Krausze J , Wamp S , Lang C , Blankenfeldt W , Flieger A |
| Ref : Proc Natl Acad Sci U S A , 118 : , 2021 |
| Abstract : |
| PubMedSearch : Diwo_2021_Proc.Natl.Acad.Sci.U.S.A_118_e2017046118 |
| PubMedID: 34074754 |
| Gene_locus related to this paper: legpn-i7i328 |
| Title : Oligomerization Inhibits Legionella pneumophila PlaB Phospholipase A Activity - Kuhle_2014_J.Biol.Chem_289_18657 |
| Author(s) : Kuhle K , Krausze J , Curth U , Rossle M , Heuner K , Lang C , Flieger A |
| Ref : Journal of Biological Chemistry , 289 :18657 , 2014 |
| Abstract : |
| PubMedSearch : Kuhle_2014_J.Biol.Chem_289_18657 |
| PubMedID: 24811180 |
| Gene_locus related to this paper: legpn-i7i328 |
| Title : Phospholipase PlaB of Legionella pneumophila represents a novel lipase family: protein residues essential for lipolytic activity, substrate specificity, and hemolysis - Bender_2009_J.Biol.Chem_284_27185 |
| Author(s) : Bender J , Rydzewski K , Broich M , Schunder E , Heuner K , Flieger A |
| Ref : Journal of Biological Chemistry , 284 :27185 , 2009 |
| Abstract : |
| PubMedSearch : Bender_2009_J.Biol.Chem_284_27185 |
| PubMedID: 19640837 |
| Gene_locus related to this paper: legpn-i7i328 , legsp-a3fmk8 |