Family: PlaB

Block: X

Parent Family: Abhydrolase_6


Bacterial phospholipases. Legionella pneumophila possesses a major cell-associated hemolytic phospholipase A (PlaB) which shares no homology to described phospholipases. PlaB preferentially hydrolyzes long-chain fatty acid substrates containing 12 or more carbon atoms. PlaB shows concentration-dependent phospholipase inactivation by tetramerization which may be a mechanism for self-protection. See Papers: Bender et al. 2009 and Kuhle et al. 2014. The tetramer is a dimer of identical dimers. Diwo et al. found in the structure eight NAD(H) molecules at the dimer/dimerinterface, suggesting that these molecules stabilize the tetramer leading to enzyme inactivation.


Interpro : No interpro


Pdoc : No Pdoc

Pfam : PF12697 Abhydrolase_6

Prints : No Print

EC Number : No EC Number


Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
6ZTH Phospholipase PlaB from Legionella pneumophila legpn-i7i328
6ZTI Phospholipase PlaB from Legionella pneumophila in complex with thio-NAD legpn-i7i328

Gene_locus Name Species
noss1-ALR3140 Anabaena sp., Nostoc sp., Anabaena variabilis, hypothetical protein alr3140 Anabaena sp.
pseae-PA2927 Pseudomonas aeruginosa hypothetical protein pa2927 PaPlaB Pseudomonas aeruginosa
solue-q01yh7 Solibacter usitatus (strain Ellin6076) Uncharacterized protein Solibacter usitatus
permh-c0qr46 Persephonella marina (strain DSM 14350 \/ EX-H1) Uncharacterized protein Persephonella marina
9alte-a6evy1 Marinobacter algicola DG893 Uncharacterized protein Marinobacter algicola DG893
9bact-m1z099 Nitrospina gracilis 3\/211 Uncharacterized protein Nitrospina gracilis 3\/211
9alte-h3zi92 Alishewanella jeotgali, aestuarii, agri, Uncharacterized protein Alishewanella jeotgali KCTC 22429
phymf-i0ibh4 Phycisphaera mikurensis (strain NBRC 102666 \/ KCTC 22515 \/ FYK2301M01) Uncharacterized protein Phycisphaera mikurensis
psepu-n9w5v8 Pseudomonas putida TRO1 Uncharacterized protein Pseudomonas putida TRO1
9gamm-w0drv6 Thioalkalivibrio thiocyanoxidans ARh 4 Uncharacterized protein Thioalkalivibrio thiocyanoxidans ARh 4
9gamm-w7q5c5 Halomonas sp. BC04 Uncharacterized protein Halomonas sp. BC04
marmm-q0arp3 Maricaulis maris (strain MCS10) Uncharacterized protein Maricaulis maris
9prot-q0exh0 Mariprofundus ferrooxydans PV-1 Phospholipase Mariprofundus ferrooxydans PV-1
legpn-i7i328 Legionella pneumophila subsp. pneumophila Legionella drancourtii, Phospholipase LpPlaB Legionella pneumophila
sheam-a1saw0 Shewanella amazonensis (strain ATCC BAA-1098 \/ SB2B) Phospholipase Shewanella amazonensis
agaal-r9psh6 Agarivorans albus MKT 106 Phospholipase Agarivorans albus MKT 106
legsp-a3fmk8 Legionella spiritensis PlaB Legionella spiritensis
9gamm-a9cwc2 Shewanella benthica KT99 Phospholipase Shewanella benthica KT99
flutr-f2ii77 Fluviicola taffensis (strain DSM 16823 \/ RW262 \/ RW262) Phospholipase Fluviicola taffensis
legln-d3hq45 Legionella longbeachae Phospholipase Legionella longbeachae NSW150
desac-q1jwt9 Desulfuromonas acetoxidans DSM 684 Phospholipase Desulfuromonas acetoxidans DSM 684
shepa-a8gza7 Shewanella pealeana (strain ATCC 700345 \/ ANG-SQ1) Phospholipase Shewanella pealeana
shevd-d4zd33 Shewanella violacea (strain JCM 10179 \/ CIP 106290 \/ LMG 19151 \/ DSS12) Uncharacterized protein Shewanella violacea
shehh-b0tju7 Shewanella halifaxensis (strain HAW-EB4) Phospholipase Shewanella halifaxensis
9gamm-i4w709 Rhodanobacter spathiphylli B39 Phospholipase Rhodanobacter spathiphylli B39
9gamm-i4w119 Rhodanobacter fulvus Jip2 Phospholipase Rhodanobacter fulvus Jip2
psyin-a1srl0 Psychromonas ingrahamii (strain 37) Phospholipase Psychromonas ingrahamii
9gamm-i4vhy2 Rhodanobacter sp. 115 Phospholipase Rhodanobacter sp. 115
shelp-a3q9j5 Shewanella loihica (strain ATCC BAA-1088 \/ PV-4) Phospholipase Shewanella loihica
9bact-b5jq87 Verrucomicrobiae bacterium DG1235 Putative uncharacterized protein Verrucomicrobiae bacterium DG1235
9gamm-m4ni10 Rhodanobacter denitrificans, Rhodanobacter thiooxydans, Uncharacterized protein Rhodanobacter denitrificans

Gene_locus_frgt Name Species
phopr-q6lki1 Photobacterium profundum (Photobacterium sp. (strain SS9)) Uncharacterized protein Photobacterium profundum
9gamm-w0dn29 Thioalkalivibrio thiocyanoxidans, Thioalkalivibrio nitratireducens, Uncharacterized protein Thioalkalivibrio thiocyanoxidans ARh 4

Substrate Chemical Nomenclature Proteins
Heptadecanoylphosphatidylcholine [(2R)-3-heptadecanoyloxy-2-hydroxypropyl] 2-(trimethylazaniumyl)ethyl phosphate pseae-PA2927    legpn-i7i328
Dipalmitoylphosphatidylglycerol [3-[2,3-dihydroxypropoxy(hydroxy)phosphoryl]oxy-2-hexadecanoyloxypropyl] hexadecanoate legpn-i7i328
Dipalmitoylphosphatidicacid [(2R)-2-hexadecanoyloxy-3-phosphonooxypropyl] hexadecanoate legpn-i7i328
Dipalmitoylphosphatidylcholine 2,3-di(hexadecanoyloxy)propyl 2-(trimethylazaniumyl)ethyl phosphate legpn-i7i328    human-LCAT

Inhibitor Chemical Nomenclature Proteins
thio-NAD [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl][[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2R,3S,4R,5R)-5-(3-carbamothioylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate legpn-i7i328

References (4)

Title : A phospholipase B from Pseudomonas aeruginosa with activity towards endogenous phospholipids affects biofilm assembly - Weiler_2022_Biochim.Biophys.Acta.Mol.Cell.Biol.Lipids_1867_159101
Author(s) : Weiler AJ , Spitz O , Gudzuhn M , Schott-Verdugo SN , Kamel M , Thiele B , Streit WR , Kedrov A , Schmitt L , Gohlk H , Kovacic F , Gohlke H
Ref : Biochimica & Biophysica Acta Molecular & Cellular Biology Lipids , 1867 :159101 , 2022
Abstract :
PubMedSearch : Weiler_2022_Biochim.Biophys.Acta.Mol.Cell.Biol.Lipids_1867_159101
PubMedID: 35063652
Gene_locus related to this paper: pseae-PA2927

Title : NAD(H)-mediated tetramerization controls the activity of Legionella pneumophila phospholipase PlaB - Diwo_2021_Proc.Natl.Acad.Sci.U.S.A_118_e2017046118
Author(s) : Diwo M , Michel W , Aurass P , Kuhle-Keindorf K , Pippel J , Krausze J , Wamp S , Lang C , Blankenfeldt W , Flieger A
Ref : Proc Natl Acad Sci U S A , 118 : , 2021
Abstract :
PubMedSearch : Diwo_2021_Proc.Natl.Acad.Sci.U.S.A_118_e2017046118
PubMedID: 34074754
Gene_locus related to this paper: legpn-i7i328

Title : Oligomerization Inhibits Legionella pneumophila PlaB Phospholipase A Activity - Kuhle_2014_J.Biol.Chem_289_18657
Author(s) : Kuhle K , Krausze J , Curth U , Rossle M , Heuner K , Lang C , Flieger A
Ref : Journal of Biological Chemistry , 289 :18657 , 2014
Abstract :
PubMedSearch : Kuhle_2014_J.Biol.Chem_289_18657
PubMedID: 24811180
Gene_locus related to this paper: legpn-i7i328

Title : Phospholipase PlaB of Legionella pneumophila represents a novel lipase family: protein residues essential for lipolytic activity, substrate specificity, and hemolysis - Bender_2009_J.Biol.Chem_284_27185
Author(s) : Bender J , Rydzewski K , Broich M , Schunder E , Heuner K , Flieger A
Ref : Journal of Biological Chemistry , 284 :27185 , 2009
Abstract :
PubMedSearch : Bender_2009_J.Biol.Chem_284_27185
PubMedID: 19640837
Gene_locus related to this paper: legpn-i7i328 , legsp-a3fmk8