legpn-i7i328

Legionella pneumophila subsp. pneumophila Legionella drancourtii, Phospholipase LpPlaB

Comment

Legionella pneumophila possesses a major cell-associated hemolytic phospholipase A (PlaB) which shares no homology to described phospholipases. PlaB preferentially hydrolyzes long-chain fatty acid substrates containing 12 or more carbon atoms. PlaB shows concentration-dependent phospholipase inactivation by tetramerization which may be a mechanism for self-protection. (See Papers Bender et al. 2009<\/A> and Kuhle et al. 2014.<\/A>). The tetramer is a dimer of identical dimers. Diwo et al. found in the structure eight NAD(H) molecules at the dimer\/dimer interface, suggesting that these molecules stabilize the tetramer leading to enzyme inactivation (Diwo et al.).The N-Terminal Phospholipase domain is a typical alpha\/beta-Hydrolase extended by the non canonical two-stranded beta-sheets beta-6\/beta-7 and beta-9\/beta-10.. Other strains: Legionella pneumophila Paris\; Leg01\/20\; Leg01\/11\; Leg01\/53\; 121004\; Thunder Bay\; Philadelphia 1 \/ ATCC 33152 \/ DSM 7513\; Lens\; Legionella drancourtii LLAP12\; ATCC 43290\; serogroup 1 (strain 2300\/99 Alcoy)\; Corby\; LPE509

Relationship

Family : PlaB

Block : X

Position in NCBI Life Tree : Legionella pneumophila

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Bacteria N E > Proteobacteria N E > Gammaproteobacteria N E > Legionellales N E > Legionellaceae N E > Legionella N E > Legionella pneumophila N E

Sequence

Peptide

MIVIFVHGWS VTHTNTYGEL PQWLENQSKQ GKLDIQVGNI YLGRYISFDD TVTVDDIARA FDQAVRDEIA DKLRDGQRFA CITHSTGGPI VRKWMDLYFK NNLAKCPLSH LIMLAPANHG SALAQLGKSR LGRIKSFFEG IEPGKCVLDW LELGSDMSWQ LNESWLDYDC TANGVYSFVL TGQKIDRQFY DAVNSYTGES GSDGVVRVAA TNMNYSLLKL HQEGDNGESL VVAKMTRTQP MAFGVLPGLS HSGKNIGIIR SITMANAATH PTAIWILRCL QVKSRDSYNK LVKELDNITK ETQKNEHKEF VKTLVFTREY ITNRYSMIIF RLIDDRGNHL IDYDLYLTAG PQYSEQALPA GFFVDRQRNL NNRGKLTYFL DYDIMEGGIN TPKMQGNLGF RVKAYPESSD QALAYYRLLD FHSSLADIHK ILHPNETVMV EIMLQRRVDR TVFRISNNLT PAKISGKPTG KKID

References (11)

Title : NAD(H)-mediated tetramerization controls the activity of Legionella pneumophila phospholipase PlaB - Diwo_2021_Proc.Natl.Acad.Sci.U.S.A_118_e2017046118
Author(s) : Diwo M , Michel W , Aurass P , Kuhle-Keindorf K , Pippel J , Krausze J , Wamp S , Lang C , Blankenfeldt W , Flieger A
Ref : Proc Natl Acad Sci U S A , 118 : , 2021
PubMedID: 34074754
Gene_locus related to this paper: legpn-i7i328

Title : Oligomerization Inhibits Legionella pneumophila PlaB Phospholipase A Activity - Kuhle_2014_J.Biol.Chem_289_18657
Author(s) : Kuhle K , Krausze J , Curth U , Rossle M , Heuner K , Lang C , Flieger A
Ref : Journal of Biological Chemistry , 289 :18657 , 2014
PubMedID: 24811180
Gene_locus related to this paper: legpn-i7i328

Title : Comparative Genomics Reveal That Host-Innate Immune Responses Influence the Clinical Prevalence of Serogroups - Khan_2013_PLoS.One_8_e67298
Author(s) : Khan MA , Knox N , Prashar A , Alexander D , Abdel-Nour M , Duncan C , Tang P , Amatullah H , Dos Santos CC , Tijet N , Low DE , Pourcel C , Van Domselaar G , Terebiznik M , Ensminger AW , Guyard C
Ref : PLoS ONE , 8 :e67298 , 2013
PubMedID: 23826259
Gene_locus related to this paper: legpa-q5x473 , legph-q5zwi2 , legpn-i7i328

Title : Genome Sequence of an Environmental Isolate of the Bacterial Pathogen Legionella pneumophila - Ma_2013_Genome.Announc_1_E00320
Author(s) : Ma J , He Y , Hu B , Luo ZQ
Ref : Genome Announc , 1 : , 2013
PubMedID: 23792742
Gene_locus related to this paper: legpa-q5x473 , legph-q5zwi2 , legpn-i7i328

Title : Whole-genome sequence of the human pathogen Legionella pneumophila serogroup 12 strain 570-CO-H - Amaro_2012_J.Bacteriol_194_1613
Author(s) : Amaro F , Gilbert JA , Owens S , Trimble W , Shuman HA
Ref : Journal of Bacteriology , 194 :1613 , 2012
PubMedID: 22374950
Gene_locus related to this paper: legph-q5zsu4 , legpa-q5x473 , legph-q5zwi2 , legpn-i7i328 , legpl-q5wsw9

Title : Insight into cross-talk between intra-amoebal pathogens - Gimenez_2011_BMC.Genomics_12_542
Author(s) : Gimenez G , Bertelli C , Moliner C , Robert C , Raoult D , Fournier PE , Greub G
Ref : BMC Genomics , 12 :542 , 2011
PubMedID: 22047552
Gene_locus related to this paper: 9gamm-g9eqk0 , legpn-i7i328 , 9gamm-g9etp4

Title : Legionella pneumophila pangenome reveals strain-specific virulence factors - D'Auria_2010_BMC.Genomics_11_181
Author(s) : D'Auria G , Jimenez-Hernandez N , Peris-Bondia F , Moya A , Latorre A
Ref : BMC Genomics , 11 :181 , 2010
PubMedID: 20236513
Gene_locus related to this paper: legph-q5zsu4 , legpa-q5x2r4 , legpa-q5x4t1 , legpa-q5x322 , legpa-q5x424 , legpa-q5x473 , legpc-a5iew3 , legph-q5zwi2 , legpl-q5wua5 , legpn-i7i328 , legpl-q5wsw9

Title : Phospholipase PlaB of Legionella pneumophila represents a novel lipase family: protein residues essential for lipolytic activity, substrate specificity, and hemolysis - Bender_2009_J.Biol.Chem_284_27185
Author(s) : Bender J , Rydzewski K , Broich M , Schunder E , Heuner K , Flieger A
Ref : Journal of Biological Chemistry , 284 :27185 , 2009
PubMedID: 19640837
Gene_locus related to this paper: legpn-i7i328 , legsp-a3fmk8

Title : Cloning and characterization of the gene encoding the major cell-associated phospholipase A of Legionella pneumophila, plaB, exhibiting hemolytic activity - Flieger_2004_Infect.Immun_72_2648
Author(s) : Flieger A , Rydzewski K , Banerji S , Broich M , Heuner K
Ref : Infect Immun , 72 :2648 , 2004
PubMedID: 15102773
Gene_locus related to this paper: legpn-i7i328