Glaser P

References (9)

Title : Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes - Hain_2012_BMC.Genomics_13_144
Author(s) : Hain T , Ghai R , Billion A , Kuenne CT , Steinweg C , Izar B , Mohamed W , Mraheil MA , Domann E , Schaffrath S , Karst U , Goesmann A , Oehm S , Puhler A , Merkl R , Vorwerk S , Glaser P , Garrido P , Rusniok C , Buchrieser C , Goebel W , Chakraborty T
Ref : BMC Genomics , 13 :144 , 2012
Abstract : BACKGROUND: Listeria monocytogenes is a food-borne pathogen that causes infections with a high-mortality rate and has served as an invaluable model for intracellular parasitism. Here, we report complete genome sequences for two L. monocytogenes strains belonging to serotype 4a (L99) and 4b (CLIP80459), and transcriptomes of representative strains from lineages I, II, and III, thereby permitting in-depth comparison of genome- and transcriptome -based data from three lineages of L. monocytogenes. Lineage III, represented by the 4a L99 genome is known to contain strains less virulent for humans.
RESULTS: The genome analysis of the weakly pathogenic L99 serotype 4a provides extensive evidence of virulence gene decay, including loss of several important surface proteins. The 4b CLIP80459 genome, unlike the previously sequenced 4b F2365 genome harbours an intact inlB invasion gene. These lineage I strains are characterized by the lack of prophage genes, as they share only a single prophage locus with other L. monocytogenes genomes 1/2a EGD-e and 4a L99. Comparative transcriptome analysis during intracellular growth uncovered adaptive expression level differences in lineages I, II and III of Listeria, notable amongst which was a strong intracellular induction of flagellar genes in strain 4a L99 compared to the other lineages. Furthermore, extensive differences between strains are manifest at levels of metabolic flux control and phosphorylated sugar uptake. Intriguingly, prophage gene expression was found to be a hallmark of intracellular gene expression. Deletion mutants in the single shared prophage locus of lineage II strain EGD-e 1/2a, the lma operon, revealed severe attenuation of virulence in a murine infection model. CONCLUSION: Comparative genomics and transcriptome analysis of L. monocytogenes strains from three lineages implicate prophage genes in intracellular adaptation and indicate that gene loss and decay may have led to the emergence of attenuated lineages.
ESTHER : Hain_2012_BMC.Genomics_13_144
PubMedSearch : Hain_2012_BMC.Genomics_13_144
PubMedID: 22530965
Gene_locus related to this paper: lismo-LMO0110 , lismo-LMO0493 , lismo-LMO0580 , lismo-LMO0950 , lismo-LMO0951 , lismo-LMO1128 , lismo-LMO1258 , lismo-LMO2089 , lismo-LMO2433 , lismo-LMO2578 , lismo-LMO2755 , lismo-metx

Title : Complete genome sequence of the animal pathogen Listeria ivanovii, which provides insights into host specificities and evolution of the genus Listeria - Buchrieser_2011_J.Bacteriol_193_6787
Author(s) : Buchrieser C , Rusniok C , Garrido P , Hain T , Scortti M , Lampidis R , Karst U , Chakraborty T , Cossart P , Kreft J , Vazquez-Boland JA , Goebel W , Glaser P
Ref : Journal of Bacteriology , 193 :6787 , 2011
Abstract : We report the complete and annotated genome sequence of the animal pathogen Listeria ivanovii subsp. ivanovii strain PAM 55 (serotype 5), isolated in 1997 in Spain from an outbreak of abortion in sheep. The sequence and its analysis are available at an interactive genome browser at the Institut Pasteur (http:\/\/
ESTHER : Buchrieser_2011_J.Bacteriol_193_6787
PubMedSearch : Buchrieser_2011_J.Bacteriol_193_6787
PubMedID: 22072644
Gene_locus related to this paper: lisip-g2zat3 , lismo-LMO2452 , lisip-g2zf96 , lisiv-a0a097bbs7

Title : Genome sequence of Streptococcus gallolyticus: insights into its adaptation to the bovine rumen and its ability to cause endocarditis - Rusniok_2010_J.Bacteriol_192_2266
Author(s) : Rusniok C , Couve E , Da Cunha V , El Gana R , Zidane N , Bouchier C , Poyart C , Leclercq R , Trieu-Cuot P , Glaser P
Ref : Journal of Bacteriology , 192 :2266 , 2010
Abstract : Streptococcus gallolyticus (formerly known as Streptococcus bovis biotype I) is an increasing cause of endocarditis among streptococci and frequently associated with colon cancer. S. gallolyticus is part of the rumen flora but also a cause of disease in ruminants as well as in birds. Here we report the complete nucleotide sequence of strain UCN34, responsible for endocarditis in a patient also suffering from colon cancer. Analysis of the 2,239 proteins encoded by its 2,350-kb-long genome revealed unique features among streptococci, probably related to its adaptation to the rumen environment and its capacity to cause endocarditis. S. gallolyticus has the capacity to use a broad range of carbohydrates of plant origin, in particular to degrade polysaccharides derived from the plant cell wall. Its genome encodes a large repertoire of transporters and catalytic activities, like tannase, phenolic compounds decarboxylase, and bile salt hydrolase, that should contribute to the detoxification of the gut environment. Furthermore, S. gallolyticus synthesizes all 20 amino acids and more vitamins than any other sequenced Streptococcus species. Many of the genes encoding these specific functions were likely acquired by lateral gene transfer from other bacterial species present in the rumen. The surface properties of strain UCN34 may also contribute to its virulence. A polysaccharide capsule might be implicated in resistance to innate immunity defenses, and glucan mucopolysaccharides, three types of pili, and collagen binding proteins may play a role in adhesion to tissues in the course of endocarditis.
ESTHER : Rusniok_2010_J.Bacteriol_192_2266
PubMedSearch : Rusniok_2010_J.Bacteriol_192_2266
PubMedID: 20139183
Gene_locus related to this paper: strg3-d3hc78 , strg3-d3hd15 , strg3-d3hem0 , strg3-d3hem1 , strg3-d3hey7

Title : NeMeSys: a biological resource for narrowing the gap between sequence and function in the human pathogen Neisseria meningitidis - Rusniok_2009_Genome.Biol_10_R110
Author(s) : Rusniok C , Vallenet D , Floquet S , Ewles H , Mouze-Soulama C , Brown D , Lajus A , Buchrieser C , Medigue C , Glaser P , Pelicic V
Ref : Genome Biol , 10 :R110 , 2009
Abstract : BACKGROUND: Genome sequences, now available for most pathogens, hold promise for the rational design of new therapies. However, biological resources for genome-scale identification of gene function (notably genes involved in pathogenesis) and/or genes essential for cell viability, which are necessary to achieve this goal, are often sorely lacking. This holds true for Neisseria meningitidis, one of the most feared human bacterial pathogens that causes meningitis and septicemia.
RESULTS: By determining and manually annotating the complete genome sequence of a serogroup C clinical isolate of N. meningitidis (strain 8013) and assembling a library of defined mutants in up to 60% of its non-essential genes, we have created NeMeSys, a biological resource for Neisseria meningitidis systematic functional analysis. To further enhance the versatility of this toolbox, we have manually (re)annotated eight publicly available Neisseria genome sequences and stored all these data in a publicly accessible online database. The potential of NeMeSys for narrowing the gap between sequence and function is illustrated in several ways, notably by performing a functional genomics analysis of the biogenesis of type IV pili, one of the most widespread virulence factors in bacteria, and by identifying through comparative genomics a complete biochemical pathway (for sulfur metabolism) that may potentially be important for nasopharyngeal colonization.
CONCLUSIONS: By improving our capacity to understand gene function in an important human pathogen, NeMeSys is expected to contribute to the ongoing efforts aimed at understanding a prokaryotic cell comprehensively and eventually to the design of new therapies.
ESTHER : Rusniok_2009_Genome.Biol_10_R110
PubMedSearch : Rusniok_2009_Genome.Biol_10_R110
PubMedID: 19818133
Gene_locus related to this paper: neimb-q9k0t9 , neime-ESD , neime-NMA2216 , neime-NMB0276 , neime-NMB1828

Title : Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity - Cazalet_2004_Nat.Genet_36_1165
Author(s) : Cazalet C , Rusniok C , Bruggemann H , Zidane N , Magnier A , Ma L , Tichit M , Jarraud S , Bouchier C , Vandenesch F , Kunst F , Etienne J , Glaser P , Buchrieser C
Ref : Nat Genet , 36 :1165 , 2004
Abstract : Legionella pneumophila, the causative agent of Legionnaires' disease, replicates as an intracellular parasite of amoebae and persists in the environment as a free-living microbe. Here we have analyzed the complete genome sequences of L. pneumophila Paris (3,503,610 bp, 3,077 genes), an endemic strain that is predominant in France, and Lens (3,345,687 bp, 2,932 genes), an epidemic strain responsible for a major outbreak of disease in France. The L. pneumophila genomes show marked plasticity, with three different plasmids and with about 13% of the sequence differing between the two strains. Only strain Paris contains a type V secretion system, and its Lvh type IV secretion system is encoded by a 36-kb region that is either carried on a multicopy plasmid or integrated into the chromosome. Genetic mobility may enhance the versatility of L. pneumophila. Numerous genes encode eukaryotic-like proteins or motifs that are predicted to modulate host cell functions to the pathogen's advantage. The genome thus reflects the history and lifestyle of L. pneumophila, a human pathogen of macrophages that coevolved with fresh-water amoebae.
ESTHER : Cazalet_2004_Nat.Genet_36_1165
PubMedSearch : Cazalet_2004_Nat.Genet_36_1165
PubMedID: 15467720
Gene_locus related to this paper: legph-q5zsu4 , legpa-q5ws33 , legpa-q5ws59 , legpa-q5ws67 , legpa-q5ws68 , legpa-q5x2c0 , legpa-q5x2r4 , legpa-q5x2s1 , legpa-q5x3a5 , legpa-q5x3d6 , legpa-q5x3j6 , legpa-q5x4r4 , legpa-q5x4t1 , legpa-q5x5b2 , legpa-q5x5z2 , legpa-q5x7f5 , legpa-q5x8e6 , legpa-q5x8m4 , legpa-q5x322 , legpa-q5x405 , legpa-q5x424 , legpa-q5x473 , legpa-q5x590 , legpa-q5x611 , legpa-q5x819 , legpc-a5iar0 , legph-q5zv00 , legph-q5zwi2 , legpl-q5wtd3 , legpl-q5wua5 , legpl-q5wur2 , legpl-q5wvw9 , legpn-Q8KU34 , legpn-Q8RNQ1 , legpn-SBPA , legpn-i7i328 , legpl-q5wsw9

Title : The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens - Duchaud_2003_Nat.Biotechnol_21_1307
Author(s) : Duchaud E , Rusniok C , Frangeul L , Buchrieser C , Givaudan A , Taourit S , Bocs S , Boursaux-Eude C , Chandler M , Charles JF , Dassa E , Derose R , Derzelle S , Freyssinet G , Gaudriault S , Medigue C , Lanois A , Powell K , Siguier P , Vincent R , Wingate V , Zouine M , Glaser P , Boemare N , Danchin A , Kunst F
Ref : Nat Biotechnol , 21 :1307 , 2003
Abstract : Photorhabdus luminescens is a symbiont of nematodes and a broad-spectrum insect pathogen. The complete genome sequence of strain TT01 is 5,688,987 base pairs (bp) long and contains 4,839 predicted protein-coding genes. Strikingly, it encodes a large number of adhesins, toxins, hemolysins, proteases and lipases, and contains a wide array of antibiotic synthesizing genes. These proteins are likely to play a role in the elimination of competitors, host colonization, invasion and bioconversion of the insect cadaver, making P. luminescens a promising model for the study of symbiosis and host-pathogen interactions. Comparison with the genomes of related bacteria reveals the acquisition of virulence factors by extensive horizontal transfer and provides clues about the evolution of an insect pathogen. Moreover, newly identified insecticidal proteins may be effective alternatives for the control of insect pests.
ESTHER : Duchaud_2003_Nat.Biotechnol_21_1307
PubMedSearch : Duchaud_2003_Nat.Biotechnol_21_1307
PubMedID: 14528314
Gene_locus related to this paper: pholl-q7maz3 , pholl-q7mb82 , pholl-q7mza3 , pholl-q7mzf6 , pholl-q7n0d9 , pholl-q7n2c6 , pholl-q7n2f0 , pholl-q7n2f7 , pholl-q7n2k4 , pholl-q7n3k0 , pholl-q7n3p5 , pholl-q7n3s1 , pholl-q7n4k8 , pholl-q7n4l0 , pholl-q7n4l7 , pholl-q7n4q6 , pholl-q7n4x6 , pholl-q7n5r3 , pholl-q7n6m7 , pholl-q7n6m8 , pholl-q7n6m9 , pholl-q7n6n0 , pholl-q7n7d3 , pholl-q7n8a5 , pholl-q7n132 , pholl-q7n239 , pholl-q7n246 , pholl-q7n258 , pholl-y1242 , pholu-BIOH , pholu-LUXD2 , pholu-PIP , pholu-PLDB , pholu-PLU0113 , pholu-PLU0399 , pholu-PLU1261 , pholu-PLU1531 , pholu-PLU1532 , pholu-PLU2160 , pholu-PLU2202 , pholu-PLU2437 , pholu-PLU3206

Title : Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease - Glaser_2002_Mol.Microbiol_45_1499
Author(s) : Glaser P , Rusniok C , Buchrieser C , Chevalier F , Frangeul L , Msadek T , Zouine M , Couve E , Lalioui L , Poyart C , Trieu-Cuot P , Kunst F
Ref : Molecular Microbiology , 45 :1499 , 2002
Abstract : Streptococcus agalactiae is a commensal bacterium colonizing the intestinal tract of a significant proportion of the human population. However, it is also a pathogen which is the leading cause of invasive infections in neonates and causes septicaemia, meningitis and pneumonia. We sequenced the genome of the serogroup III strain NEM316, responsible for a fatal case of septicaemia. The genome is 2 211 485 base pairs long and contains 2118 protein coding genes. Fifty-five per cent of the predicted genes have an ortholog in the Streptococcus pyogenes genome, representing a conserved backbone between these two streptococci. Among the genes in S. agalactiae that lack an ortholog in S. pyogenes, 50% are clustered within 14 islands. These islands contain known and putative virulence genes, mostly encoding surface proteins as well as a number of genes related to mobile elements. Some of these islands could therefore be considered as pathogenicity islands. Compared with other pathogenic streptococci, S. agalactiae shows the unique feature that pathogenicity islands may have an important role in virulence acquisition and in genetic diversity.
ESTHER : Glaser_2002_Mol.Microbiol_45_1499
PubMedSearch : Glaser_2002_Mol.Microbiol_45_1499
PubMedID: 12354221
Gene_locus related to this paper: strag-ESTA , strag-GBS0040 , strag-GBS0107 , strag-GBS0567 , strag-GBS1828 , strag-GBS1967 , strag-pepx , strag-SAG0383 , strag-SAG0785 , strag-SAG0912 , strag-SAG1040 , strag-SAG1562 , strag-SAG2132

Title : Comparative genomics of Listeria species - Glaser_2001_Science_294_849
Author(s) : Glaser P , Frangeul L , Buchrieser C , Rusniok C , Amend A , Baquero F , Berche P , Bloecker H , Brandt P , Chakraborty T , Charbit A , Chetouani F , Couve E , de Daruvar A , Dehoux P , Domann E , Dominguez-Bernal G , Duchaud E , Durant L , Dussurget O , Entian KD , Fsihi H , Portillo FG , Garrido P , Gautier L , Goebel W , Gomez-Lopez N , Hain T , Hauf J , Jackson D , Jones LM , Kaerst U , Kreft J , Kuhn M , Kunst F , Kurapkat G , Madueno E , Maitournam A , Vicente JM , Ng E , Nedjari H , Nordsiek G , Novella S , de Pablos B , Perez-Diaz JC , Purcell R , Remmel B , Rose M , Schlueter T , Simoes N , Tierrez A , Vazquez-Boland JA , Voss H , Wehland J , Cossart P
Ref : Science , 294 :849 , 2001
Abstract : Listeria monocytogenes is a food-borne pathogen with a high mortality rate that has also emerged as a paradigm for intracellular parasitism. We present and compare the genome sequences of L. monocytogenes (2,944,528 base pairs) and a nonpathogenic species, L. innocua (3,011,209 base pairs). We found a large number of predicted genes encoding surface and secreted proteins, transporters, and transcriptional regulators, consistent with the ability of both species to adapt to diverse environments. The presence of 270 L. monocytogenes and 149 L. innocua strain-specific genes (clustered in 100 and 63 islets, respectively) suggests that virulence in Listeria results from multiple gene acquisition and deletion events.
ESTHER : Glaser_2001_Science_294_849
PubMedSearch : Glaser_2001_Science_294_849
PubMedID: 11679669
Gene_locus related to this paper: lisin-LIN0589 , lisin-LIN0754 , lisin-LIN0850 , lisin-LIN0949 , lisin-LIN0950 , lisin-LIN0976 , lisin-LIN1094 , lisin-LIN1546 , lisin-LIN1782 , lisin-LIN2180 , lisin-LIN2214 , lisin-LIN2363 , lisin-LIN2527 , lisin-LIN2544 , lisin-LIN2547 , lisin-LIN2722 , lisin-LIN2825 , lisin-LIN2898 , lismc-c1l0d9 , lismo-LMO0110 , lismo-LMO0493 , lismo-LMO0580 , lismo-LMO0752 , lismo-LMO0760 , lismo-LMO0857 , lismo-LMO0950 , lismo-LMO0951 , lismo-LMO0977 , lismo-LMO1128 , lismo-LMO1258 , lismo-LMO1511 , lismo-LMO1674 , lismo-LMO2074 , lismo-LMO2089 , lismo-LMO2109 , lismo-LMO2262 , lismo-LMO2433 , lismo-LMO2450 , lismo-LMO2452 , lismo-LMO2453 , lismo-LMO2578 , lismo-LMO2677 , lismo-LMO2755 , lismo-metx

Title : The complete genome sequence of the gram-positive bacterium Bacillus subtilis - Kunst_1997_Nature_390_249
Author(s) : Kunst F , Ogasawara N , Moszer I , Albertini AM , Alloni G , Azevedo V , Bertero MG , Bessieres P , Bolotin A , Borchert S , Borriss R , Boursier L , Brans A , Braun M , Brignell SC , Bron S , Brouillet S , Bruschi CV , Caldwell B , Capuano V , Carter NM , Choi SK , Cordani JJ , Connerton IF , Cummings NJ , Daniel RA , Denziot F , Devine KM , Dusterhoft A , Ehrlich SD , Emmerson PT , Entian KD , Errington J , Fabret C , Ferrari E , Foulger D , Fritz C , Fujita M , Fujita Y , Fuma S , Galizzi A , Galleron N , Ghim SY , Glaser P , Goffeau A , Golightly EJ , Grandi G , Guiseppi G , Guy BJ , Haga K , Haiech J , Harwood CR , Henaut A , Hilbert H , Holsappel S , Hosono S , Hullo MF , Itaya M , Jones L , Joris B , Karamata D , Kasahara Y , Klaerr-Blanchard M , Klein C , Kobayashi Y , Koetter P , Koningstein G , Krogh S , Kumano M , Kurita K , Lapidus A , Lardinois S , Lauber J , Lazarevic V , Lee SM , Levine A , Liu H , Masuda S , Mauel C , Medigue C , Medina N , Mellado RP , Mizuno M , Moestl D , Nakai S , Noback M , Noone D , O'Reilly M , Ogawa K , Ogiwara A , Oudega B , Park SH , Parro V , Pohl TM , Portelle D , Porwollik S , Prescott AM , Presecan E , Pujic P , Purnelle B , Rapoport G , Rey M , Reynolds S , Rieger M , Rivolta C , Rocha E , Roche B , Rose M , Sadaie Y , Sato T , Scanlan E , Schleich S , Schroeter R , Scoffone F , Sekiguchi J , Sekowska A , Seror SJ , Serror P , Shin BS , Soldo B , Sorokin A , Tacconi E , Takagi T , Takahashi H , Takemaru K , Takeuchi M , Tamakoshi A , Tanaka T , Terpstra P , Togoni A , Tosato V , Uchiyama S , Vandebol M , Vannier F , Vassarotti A , Viari A , Wambutt R , Wedler H , Weitzenegger T , Winters P , Wipat A , Yamamoto H , Yamane K , Yasumoto K , Yata K , Yoshida K , Yoshikawa HF , Zumstein E , Yoshikawa H , Danchin A
Ref : Nature , 390 :249 , 1997
Abstract : Bacillus subtilis is the best-characterized member of the Gram-positive bacteria. Its genome of 4,214,810 base pairs comprises 4,100 protein-coding genes. Of these protein-coding genes, 53% are represented once, while a quarter of the genome corresponds to several gene families that have been greatly expanded by gene duplication, the largest family containing 77 putative ATP-binding transport proteins. In addition, a large proportion of the genetic capacity is devoted to the utilization of a variety of carbon sources, including many plant-derived molecules. The identification of five signal peptidase genes, as well as several genes for components of the secretion apparatus, is important given the capacity of Bacillus strains to secrete large amounts of industrially important enzymes. Many of the genes are involved in the synthesis of secondary metabolites, including antibiotics, that are more typically associated with Streptomyces species. The genome contains at least ten prophages or remnants of prophages, indicating that bacteriophage infection has played an important evolutionary role in horizontal gene transfer, in particular in the propagation of bacterial pathogenesis.
ESTHER : Kunst_1997_Nature_390_249
PubMedSearch : Kunst_1997_Nature_390_249
PubMedID: 9384377
Gene_locus related to this paper: bacsu-CAH , bacsu-cbxnp , bacsu-lip , bacsu-LIPB , bacsu-PKSR , bacsu-pnbae , bacsu-PPSE , bacsu-srf4 , bacsu-srfac , bacsu-YBAC , bacsu-YBDG , bacsu-ybfk , bacsu-ycgS , bacsu-yczh , bacsu-YDEN , bacsu-ydjp , bacsu-yfhM , bacsu-yisY , bacsu-YITV , bacsu-yjau , bacsu-YJCH , bacsu-MHQD , bacsu-yqjl , bacsu-yqkd , bacsu-YRAK , bacsu-YTAP , bacsu-YTMA , bacsu-YTPA , bacsu-ytxm , bacsu-yugF , bacsu-YUII , bacsu-YUKL , bacsu-YVAK , bacsu-YvaM , bacsu-RsbQ