Brettin TS

References (22)

Title : Complete genome sequence of the Antarctic Halorubrum lacusprofundi type strain ACAM 34 - Anderson_2016_Stand.Genomic.Sci_11_70
Author(s) : Anderson IJ , DasSarma P , Lucas S , Copeland A , Lapidus A , Del Rio TG , Tice H , Dalin E , Bruce DC , Goodwin L , Pitluck S , Sims D , Brettin TS , Detter JC , Han CS , Larimer F , Hauser L , Land M , Ivanova N , Richardson P , Cavicchioli R , DasSarma S , Woese CR , Kyrpides NC
Ref : Stand Genomic Sci , 11 :70 , 2016
Abstract : Halorubrum lacusprofundi is an extreme halophile within the archaeal phylum Euryarchaeota. The type strain ACAM 34 was isolated from Deep Lake, Antarctica. H. lacusprofundi is of phylogenetic interest because it is distantly related to the haloarchaea that have previously been sequenced. It is also of interest because of its psychrotolerance. We report here the complete genome sequence of H. lacusprofundi type strain ACAM 34 and its annotation. This genome is part of a 2006 Joint Genome Institute Community Sequencing Program project to sequence genomes of diverse Archaea.
ESTHER : Anderson_2016_Stand.Genomic.Sci_11_70
PubMedSearch : Anderson_2016_Stand.Genomic.Sci_11_70
PubMedID: 27617060
Gene_locus related to this paper: hallt-metxa

Title : Temperature regulation of virulence factors in the pathogen Vibrio coralliilyticus - Kimes_2012_ISME.J_6_835
Author(s) : Kimes NE , Grim CJ , Johnson WR , Hasan NA , Tall BD , Kothary MH , Kiss H , Munk AC , Tapia R , Green L , Detter C , Bruce DC , Brettin TS , Colwell RR , Morris PJ
Ref : Isme J , 6 :835 , 2012
Abstract : Sea surface temperatures (SST) are rising because of global climate change. As a result, pathogenic Vibrio species that infect humans and marine organisms during warmer summer months are of growing concern. Coral reefs, in particular, are already experiencing unprecedented degradation worldwide due in part to infectious disease outbreaks and bleaching episodes that are exacerbated by increasing SST. For example, Vibrio coralliilyticus, a globally distributed bacterium associated with multiple coral diseases, infects corals at temperatures above 27 degrees C. The mechanisms underlying this temperature-dependent pathogenicity, however, are unknown. In this study, we identify potential virulence mechanisms using whole genome sequencing of V. coralliilyticus ATCC (American Type Culture Collection) BAA-450. Furthermore, we demonstrate direct temperature regulation of numerous virulence factors using proteomic analysis and bioassays. Virulence factors involved in motility, host degradation, secretion, antimicrobial resistance and transcriptional regulation are upregulated at the higher virulent temperature of 27 degrees C, concurrent with phenotypic changes in motility, antibiotic resistance, hemolysis, cytotoxicity and bioluminescence. These results provide evidence that temperature regulates multiple virulence mechanisms in V. coralliilyticus, independent of abundance. The ecological and biological significance of this temperature-dependent virulence response is reinforced by climate change models that predict tropical SST to consistently exceed 27 degrees C during the spring, summer and fall seasons. We propose V. coralliilyticus as a model Gram-negative bacterium to study temperature-dependent pathogenicity in Vibrio-related diseases.
ESTHER : Kimes_2012_ISME.J_6_835
PubMedSearch : Kimes_2012_ISME.J_6_835
PubMedID: 22158392
Gene_locus related to this paper: 9vibr-c9nnl3 , 9vibr-c9nqc3 , 9vibr-c9ntb8 , vibcr-c9nnv6 , 9vibr-u0f626

Title : Genome sequence of Pedosphaera parvula Ellin514, an aerobic Verrucomicrobial isolate from pasture soil - Kant_2011_J.Bacteriol_193_2900
Author(s) : Kant R , van Passel MW , Sangwan P , Palva A , Lucas S , Copeland A , Lapidus A , Glavina Del Rio T , Dalin E , Tice H , Bruce D , Goodwin L , Pitluck S , Chertkov O , Larimer FW , Land ML , Hauser L , Brettin TS , Detter JC , Han S , de Vos WM , Janssen PH , Smidt H
Ref : Journal of Bacteriology , 193 :2900 , 2011
Abstract : "Pedosphaera parvula" Ellin514 is an aerobically grown verrucomicrobial isolate from pasture soil. It is one of the few cultured representatives of subdivision 3 of the phylum Verrucomicrobia. Members of this group are widespread in terrestrial environments.
ESTHER : Kant_2011_J.Bacteriol_193_2900
PubMedSearch : Kant_2011_J.Bacteriol_193_2900
PubMedID: 21460084
Gene_locus related to this paper: 9bact-b9xah7.1 , 9bact-b9xah7.2 , 9bact-b9xba2 , 9bact-b9xfz4 , 9bact-b9xh19 , 9bact-b9xhn8 , 9bact-b9xj62 , 9bact-b9xjh1 , 9bact-b9xku6 , 9bact-b9xku8 , 9bact-b9xnx1 , 9bact-b9xp64 , 9bact-b9xp74

Title : Genome sequence of the ethanol-producing Zymomonas mobilis subsp. pomaceae lectotype strain ATCC 29192 - Kouvelis_2011_J.Bacteriol_193_5049
Author(s) : Kouvelis VN , Davenport KW , Brettin TS , Bruce D , Detter C , Han CS , Nolan M , Tapia R , Damoulaki A , Kyrpides NC , Typas MA , Pappas KM
Ref : Journal of Bacteriology , 193 :5049 , 2011
Abstract : Zymomonas mobilis is an alphaproteobacterium studied for bioethanol production. Different strains of this organism have been hitherto sequenced; they all belong to the Z. mobilis subsp. mobilis taxon. Here we report the finished and annotated genome sequence of strain ATCC 29192, a cider-spoiling agent isolated in the United Kingdom. ATCC 29192 is the lectotype of the second-best-characterized subspecies of Z. mobilis, Z. mobilis subsp. pomaceae. The nucleotide sequence of ATCC 29192 deviates from that of Z. mobilis subsp. mobilis representatives, which justifies its distinct taxonomic positioning and proves particularly useful for comparative and functional genomic analyses.
ESTHER : Kouvelis_2011_J.Bacteriol_193_5049
PubMedSearch : Kouvelis_2011_J.Bacteriol_193_5049
PubMedID: 21742897
Gene_locus related to this paper: zymmt-f8eue4

Title : Genome sequence of Victivallis vadensis ATCC BAA-548, an anaerobic bacterium from the phylum Lentisphaerae, isolated from the human gastrointestinal tract - van Passel_2011_J.Bacteriol_193_2373
Author(s) : van Passel MW , Kant R , Palva A , Lucas S , Copeland A , Lapidus A , Glavina Del Rio T , Dalin E , Tice H , Bruce D , Goodwin L , Pitluck S , Davenport KW , Sims D , Brettin TS , Detter JC , Han S , Larimer FW , Land ML , Hauser L , Kyrpides N , Ovchinnikova G , Richardson PP , de Vos WM , Smidt H , Zoetendal EG
Ref : Journal of Bacteriology , 193 :2373 , 2011
Abstract : Victivallis vadensis ATCC BAA-548 represents the first cultured representative from the novel phylum Lentisphaerae, a deep-branching bacterial lineage. Few cultured bacteria from this phylum are known, and V. vadensis therefore represents an important organism for evolutionary studies. V. vadensis is a strictly anaerobic sugar-fermenting isolate from the human gastrointestinal tract.
ESTHER : van Passel_2011_J.Bacteriol_193_2373
PubMedSearch : van Passel_2011_J.Bacteriol_193_2373
PubMedID: 21398537
Gene_locus related to this paper: 9bact-d1n3e0 , 9bact-d1n3w1 , 9bact-d1n5u2 , 9bact-d1n6r9 , 9bact-d1n8l2 , 9bact-d1n8z8 , 9bact-d1n9n1 , 9bact-d1n9u1 , 9bact-d1n752 , 9bact-d1n881 , 9bact-d1naa7 , 9bact-d1nb62 , 9bact-d1nbd5 , 9bact-d1nbg1 , 9bact-d1nbh9 , 9bact-d1nbv9

Title : Genome sequence of the ethanol-producing Zymomonas mobilis subsp. mobilis lectotype strain ATCC 10988 - Pappas_2011_J.Bacteriol_193_5051
Author(s) : Pappas KM , Kouvelis VN , Saunders E , Brettin TS , Bruce D , Detter C , Balakireva M , Han CS , Savvakis G , Kyrpides NC , Typas MA
Ref : Journal of Bacteriology , 193 :5051 , 2011
Abstract : Zymomonas mobilis ATCC 10988 is the type strain of the Z. mobilis subsp. mobilis taxon, members of which are some of the most rigorous ethanol-producing bacteria. Isolated from Agave cactus fermentations in Mexico, ATCC 10988 is one of the first Z. mobilis strains to be described and studied. Its robustness in sucrose-substrate fermentations, physiological characteristics, large number of plasmids, and overall genomic plasticity render this strain important to the study of the species. Here we report the finishing and annotation of the ATCC 10988 chromosomal and plasmid genome.
ESTHER : Pappas_2011_J.Bacteriol_193_5051
PubMedSearch : Pappas_2011_J.Bacteriol_193_5051
PubMedID: 21725006
Gene_locus related to this paper: zymmo-q5nmh0 , zymma-a0a0h3g4v2

Title : Genome Sequence of the ethene- and vinyl chloride-oxidizing actinomycete Nocardioides sp. strain JS614 - Coleman_2011_J.Bacteriol_193_3399
Author(s) : Coleman NV , Wilson NL , Barry K , Brettin TS , Bruce DC , Copeland A , Dalin E , Detter JC , Del Rio TG , Goodwin LA , Hammon NM , Han S , Hauser LJ , Israni S , Kim E , Kyrpides N , Land ML , Lapidus A , Larimer FW , Lucas S , Pitluck S , Richardson P , Schmutz J , Tapia R , Thompson S , Tice HN , Spain JC , Gossett JG , Mattes TE
Ref : Journal of Bacteriology , 193 :3399 , 2011
Abstract : Nocardioides sp. strain JS614 grows on ethene and vinyl chloride (VC) as sole carbon and energy sources and is of interest for bioremediation and biocatalysis. Sequencing of the complete genome of JS614 provides insight into the genetic basis of alkene oxidation, supports ongoing research into the physiology and biochemistry of growth on ethene and VC, and provides biomarkers to facilitate detection of VC/ethene oxidizers in the environment. This is the first genome sequence from the genus Nocardioides and the first genome of a VC/ethene-oxidizing bacterium.
ESTHER : Coleman_2011_J.Bacteriol_193_3399
PubMedSearch : Coleman_2011_J.Bacteriol_193_3399
PubMedID: 21551312
Gene_locus related to this paper: nocsj-a1sil5

Title : Large direct repeats flank genomic rearrangements between a new clinical isolate of Francisella tularensis subsp. tularensis A1 and Schu S4 - Nalbantoglu_2010_PLoS.One_5_e9007
Author(s) : Nalbantoglu U , Sayood K , Dempsey MP , Iwen PC , Francesconi SC , Barabote RD , Xie G , Brettin TS , Hinrichs SH , Fey PD
Ref : PLoS ONE , 5 :e9007 , 2010
Abstract : Francisella tularensis subspecies tularensis consists of two separate populations A1 and A2. This report describes the complete genome sequence of NE061598, an F. tularensis subspecies tularensis A1 isolated in 1998 from a human with clinical disease in Nebraska, United States of America. The genome sequence was compared to Schu S4, an F. tularensis subspecies tularensis A1a strain originally isolated in Ohio in 1941. It was determined that there were 25 nucleotide polymorphisms (22 SNPs and 3 indels) between Schu S4 and NE061598; two of these polymorphisms were in potential virulence loci. Pulsed-field gel electrophoresis analysis demonstrated that NE061598 was an A1a genotype. Other differences included repeat sequences (n = 11 separate loci), four of which were contained in coding sequences, and an inversion and rearrangement probably mediated by insertion sequences and the previously identified direct repeats I, II, and III. Five new variable-number tandem repeats were identified; three of these five were unique in NE061598 compared to Schu S4. Importantly, there was no gene loss or gain identified between NE061598 and Schu S4. Interpretation of these data suggests there is significant sequence conservation and chromosomal synteny within the A1 population. Further studies are needed to determine the biological properties driving the selective pressure that maintains the chromosomal structure of this monomorphic pathogen.
ESTHER : Nalbantoglu_2010_PLoS.One_5_e9007
PubMedSearch : Nalbantoglu_2010_PLoS.One_5_e9007
PubMedID: 20140244
Gene_locus related to this paper: fratt-q5ngu5

Title : Comparative genomics of clinical and environmental Vibrio mimicus - Hasan_2010_Proc.Natl.Acad.Sci.U.S.A_107_21134
Author(s) : Hasan NA , Grim CJ , Haley BJ , Chun J , Alam M , Taviani E , Hoq M , Munk AC , Saunders E , Brettin TS , Bruce DC , Challacombe JF , Detter JC , Han CS , Xie G , Nair GB , Huq A , Colwell RR
Ref : Proc Natl Acad Sci U S A , 107 :21134 , 2010
Abstract : Whether Vibrio mimicus is a variant of Vibrio cholerae or a separate species has been the subject of taxonomic controversy. A genomic analysis was undertaken to resolve the issue. The genomes of V. mimicus MB451, a clinical isolate, and VM223, an environmental isolate, comprise ca. 4,347,971 and 4,313,453 bp and encode 3,802 and 3,290 ORFs, respectively. As in other vibrios, chromosome I (C-I) predominantly contains genes necessary for growth and viability, whereas chromosome II (C-II) bears genes for adaptation to environmental change. C-I harbors many virulence genes, including some not previously reported in V. mimicus, such as mannose-sensitive hemagglutinin (MSHA), and enterotoxigenic hemolysin (HlyA); C-II encodes a variant of Vibrio pathogenicity island 2 (VPI-2), and Vibrio seventh pandemic island II (VSP-II) cluster of genes. Extensive genomic rearrangement in C-II indicates it is a hot spot for evolution and genesis of speciation for the genus Vibrio. The number of virulence regions discovered in this study (VSP-II, MSHA, HlyA, type IV pilin, PilE, and integron integrase, IntI4) with no notable difference in potential virulence genes between clinical and environmental strains suggests these genes also may play a role in the environment and that pathogenic strains may arise in the environment. Significant genome synteny with prototypic pre-seventh pandemic strains of V. cholerae was observed, and the results of phylogenetic analysis support the hypothesis that, in the course of evolution, V. mimicus and V. cholerae diverged from a common ancestor with a prototypic sixth pandemic genomic backbone.
ESTHER : Hasan_2010_Proc.Natl.Acad.Sci.U.S.A_107_21134
PubMedSearch : Hasan_2010_Proc.Natl.Acad.Sci.U.S.A_107_21134
PubMedID: 21078967
Gene_locus related to this paper: vibch-VC2610 , vibch-VC2718 , vibch-VCA0688 , vibch-y1892 , vibch-y2276 , vibmi-d0gt41 , vibmi-u4zh77

Title : Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils - Ward_2009_Appl.Environ.Microbiol_75_2046
Author(s) : Ward NL , Challacombe JF , Janssen PH , Henrissat B , Coutinho PM , Wu M , Xie G , Haft DH , Sait M , Badger J , Barabote RD , Bradley B , Brettin TS , Brinkac LM , Bruce D , Creasy T , Daugherty SC , Davidsen TM , DeBoy RT , Detter JC , Dodson RJ , Durkin AS , Ganapathy A , Gwinn-Giglio M , Han CS , Khouri H , Kiss H , Kothari SP , Madupu R , Nelson KE , Nelson WC , Paulsen I , Penn K , Ren Q , Rosovitz MJ , Selengut JD , Shrivastava S , Sullivan SA , Tapia R , Thompson LS , Watkins KL , Yang Q , Yu C , Zafar N , Zhou L , Kuske CR
Ref : Applied Environmental Microbiology , 75 :2046 , 2009
Abstract : The complete genomes of three strains from the phylum Acidobacteria were compared. Phylogenetic analysis placed them as a unique phylum. They share genomic traits with members of the Proteobacteria, the Cyanobacteria, and the Fungi. The three strains appear to be versatile heterotrophs. Genomic and culture traits indicate the use of carbon sources that span simple sugars to more complex substrates such as hemicellulose, cellulose, and chitin. The genomes encode low-specificity major facilitator superfamily transporters and high-affinity ABC transporters for sugars, suggesting that they are best suited to low-nutrient conditions. They appear capable of nitrate and nitrite reduction but not N(2) fixation or denitrification. The genomes contained numerous genes that encode siderophore receptors, but no evidence of siderophore production was found, suggesting that they may obtain iron via interaction with other microorganisms. The presence of cellulose synthesis genes and a large class of novel high-molecular-weight excreted proteins suggests potential traits for desiccation resistance, biofilm formation, and/or contribution to soil structure. Polyketide synthase and macrolide glycosylation genes suggest the production of novel antimicrobial compounds. Genes that encode a variety of novel proteins were also identified. The abundance of acidobacteria in soils worldwide and the breadth of potential carbon use by the sequenced strains suggest significant and previously unrecognized contributions to the terrestrial carbon cycle. Combining our genomic evidence with available culture traits, we postulate that cells of these isolates are long-lived, divide slowly, exhibit slow metabolic rates under low-nutrient conditions, and are well equipped to tolerate fluctuations in soil hydration.
ESTHER : Ward_2009_Appl.Environ.Microbiol_75_2046
PubMedSearch : Ward_2009_Appl.Environ.Microbiol_75_2046
PubMedID: 19201974
Gene_locus related to this paper: korve-q1ihr9 , korve-q1ii02 , korve-q1iit0 , korve-q1ilk4 , korve-q1imj9 , korve-q1ims4 , korve-q1iqj0 , korve-q1isy7 , korve-q1itj5 , korve-q1itz6 , korve-q1ivc8 , acic5-c1f1u6 , acic5-c1f2i7 , acic5-c1f4m6 , acic5-c1f4y4 , acic5-c1f5a7 , acic5-c1f5u2 , acic5-c1f7a9 , acic5-c1f7x6 , acic5-c1f8y9 , acic5-c1f9m2 , acic5-c1f594 , acic5-c1f609 , acic5-c1f692 , acic5-c1f970 , acic5-c1fa52 , korve-q1iiw2 , korve-q1ivn9 , solue-q01nb0 , solue-q01qj6 , solue-q01r37 , solue-q01rq8 , solue-q01rz0 , solue-q01t44 , solue-q01t57 , solue-q01ts5 , solue-q01tv4 , solue-q01vd8 , solue-q01vr3 , solue-q01vw5 , solue-q01w12 , solue-q01wt9 , solue-q01y40 , solue-q01ym8 , solue-q01z24 , solue-q01z97 , solue-q01zl4 , solue-q01zm0 , solue-q01zm5 , solue-q01zm7 , solue-q02aa4 , solue-q02ab9 , solue-q02b72 , solue-q02bs8 , solue-q02bt7 , solue-q02cp0 , solue-q02d61 , solue-q020h3 , solue-q020i8 , solue-q021i6 , solue-q022b1 , solue-q022p8 , solue-q022q2 , solue-q022q3 , solue-q022x2 , solue-q022x5 , solue-q022x6 , solue-q022x8 , solue-q023e7 , solue-q024d9 , solue-q025c1 , solue-q026j1 , solue-q026k6 , solue-q026r6 , solue-q027p2 , solue-q027r8 , solue-q01zt5 , korve-q1itw6 , solue-q01yh7 , solue-q02ad6 , korve-q1imj6 , korve-q1iuf6 , acic5-c1f891 , solue-q026h7

Title : Complete genome sequence of Francisella tularensis subspecies holarctica FTNF002-00 - Barabote_2009_PLoS.One_4_e7041
Author(s) : Barabote RD , Xie G , Brettin TS , Hinrichs SH , Fey PD , Jay JJ , Engle JL , Godbole SD , Noronha JM , Scheuermann RH , Zhou LW , Lion C , Dempsey MP
Ref : PLoS ONE , 4 :e7041 , 2009
Abstract : Francisella tularensis subspecies holarctica FTNF002-00 strain was originally obtained from the first known clinical case of bacteremic F. tularensis pneumonia in Southern Europe isolated from an immunocompetent individual. The FTNF002-00 complete genome contains the RD(23) deletion and represents a type strain for a clonal population from the first epidemic tularemia outbreak in Spain between 1997-1998. Here, we present the complete sequence analysis of the FTNF002-00 genome. The complete genome sequence of FTNF002-00 revealed several large as well as small genomic differences with respect to two other published complete genome sequences of F. tularensis subsp. holarctica strains, LVS and OSU18. The FTNF002-00 genome shares >99.9% sequence similarity with LVS and OSU18, and is also approximately 5 MB smaller by comparison. The overall organization of the FTNF002-00 genome is remarkably identical to those of LVS and OSU18, except for a single 3.9 kb inversion in FTNF002-00. Twelve regions of difference ranging from 0.1-1.5 kb and forty-two small insertions and deletions were identified in a comparative analysis of FTNF002-00, LVS, and OSU18 genomes. Two small deletions appear to inactivate two genes in FTNF002-00 causing them to become pseudogenes; the intact genes encode a protein of unknown function and a drug:H(+) antiporter. In addition, we identified ninety-nine proteins in FTNF002-00 containing amino acid mutations compared to LVS and OSU18. Several non-conserved amino acid replacements were identified, one of which occurs in the virulence-associated intracellular growth locus subunit D protein. Many of these changes in FTNF002-00 are likely the consequence of direct selection that increases the fitness of this subsp. holarctica clone within its endemic population. Our complete genome sequence analyses lay the foundation for experimental testing of these possibilities.
ESTHER : Barabote_2009_PLoS.One_4_e7041
PubMedSearch : Barabote_2009_PLoS.One_4_e7041
PubMedID: 19756146
Gene_locus related to this paper: fratt-q5ngu5

Title : Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens - Silby_2009_Genome.Biol_10_R51
Author(s) : Silby MW , Cerdeno-Tarraga AM , Vernikos GS , Giddens SR , Jackson RW , Preston GM , Zhang XX , Moon CD , Gehrig SM , Godfrey SA , Knight CG , Malone JG , Robinson Z , Spiers AJ , Harris S , Challis GL , Yaxley AM , Harris D , Seeger K , Murphy L , Rutter S , Squares R , Quail MA , Saunders E , Mavromatis K , Brettin TS , Bentley SD , Hothersall J , Stephens E , Thomas CM , Parkhill J , Levy SB , Rainey PB , Thomson NR
Ref : Genome Biol , 10 :R51 , 2009
Abstract : BACKGROUND: Pseudomonas fluorescens are common soil bacteria that can improve plant health through nutrient cycling, pathogen antagonism and induction of plant defenses. The genome sequences of strains SBW25 and Pf0-1 were determined and compared to each other and with P. fluorescens Pf-5. A functional genomic in vivo expression technology (IVET) screen provided insight into genes used by P. fluorescens in its natural environment and an improved understanding of the ecological significance of diversity within this species. RESULTS: Comparisons of three P. fluorescens genomes (SBW25, Pf0-1, Pf-5) revealed considerable divergence: 61% of genes are shared, the majority located near the replication origin. Phylogenetic and average amino acid identity analyses showed a low overall relationship. A functional screen of SBW25 defined 125 plant-induced genes including a range of functions specific to the plant environment. Orthologues of 83 of these exist in Pf0-1 and Pf-5, with 73 shared by both strains. The P. fluorescens genomes carry numerous complex repetitive DNA sequences, some resembling Miniature Inverted-repeat Transposable Elements (MITEs). In SBW25, repeat density and distribution revealed 'repeat deserts' lacking repeats, covering approximately 40% of the genome. CONCLUSIONS: P. fluorescens genomes are highly diverse. Strain-specific regions around the replication terminus suggest genome compartmentalization. The genomic heterogeneity among the three strains is reminiscent of a species complex rather than a single species. That 42% of plant-inducible genes were not shared by all strains reinforces this conclusion and shows that ecological success requires specialized and core functions. The diversity also indicates the significant size of genetic information within the Pseudomonas pan genome.
ESTHER : Silby_2009_Genome.Biol_10_R51
PubMedSearch : Silby_2009_Genome.Biol_10_R51
PubMedID: 19432983
Gene_locus related to this paper: psef5-metx , psefl-este , psefs-c3jz63 , psefs-c3jzq8 , psefs-c3k1v7 , psefs-c3k3d6 , psefs-c3k3m8 , psefs-c3k3s9 , psefs-c3k5q5 , psefs-c3k6c6 , psefs-c3k7v4 , psefs-c3k8m7 , psefs-c3k8y7 , psefs-c3k9z2 , psefs-c3k032 , psefs-c3k320 , psefs-c3k362 , psefs-c3k632 , psefs-c3k927 , psefs-c3kan9 , psefs-c3kbe5 , psefs-c3kdh9 , psefs-c3ke34 , psefs-laaa , psepf-q3k5t9 , psepf-q3k6f3 , psepf-q3k524 , psepf-q3kcu9 , psepf-q3kd07 , psepf-q3kf33 , psepf-q3kh87 , psefs-c3jxp6 , psepf-q3kf85 , psefs-c3k813 , psefs-c3keb8 , psefl-e2xkc8 , psefs-c3k9x6

Title : Comparative genomics reveals mechanism for short-term and long-term clonal transitions in pandemic Vibrio cholerae - Chun_2009_Proc.Natl.Acad.Sci.U.S.A_106_15442
Author(s) : Chun J , Grim CJ , Hasan NA , Lee JH , Choi SY , Haley BJ , Taviani E , Jeon YS , Kim DW , Brettin TS , Bruce DC , Challacombe JF , Detter JC , Han CS , Munk AC , Chertkov O , Meincke L , Saunders E , Walters RA , Huq A , Nair GB , Colwell RR
Ref : Proc Natl Acad Sci U S A , 106 :15442 , 2009
Abstract : Vibrio cholerae, the causative agent of cholera, is a bacterium autochthonous to the aquatic environment, and a serious public health threat. V. cholerae serogroup O1 is responsible for the previous two cholera pandemics, in which classical and El Tor biotypes were dominant in the sixth and the current seventh pandemics, respectively. Cholera researchers continually face newly emerging and reemerging pathogenic clones carrying diverse combinations of phenotypic and genotypic properties, which significantly hampered control of the disease. To elucidate evolutionary mechanisms governing genetic diversity of pandemic V. cholerae, we compared the genome sequences of 23 V. cholerae strains isolated from a variety of sources over the past 98 years. The genome-based phylogeny revealed 12 distinct V. cholerae lineages, of which one comprises both O1 classical and El Tor biotypes. All seventh pandemic clones share nearly identical gene content. Using analogy to influenza virology, we define the transition from sixth to seventh pandemic strains as a "shift" between pathogenic clones belonging to the same O1 serogroup, but from significantly different phyletic lineages. In contrast, transition among clones during the present pandemic period is characterized as a "drift" between clones, differentiated mainly by varying composition of laterally transferred genomic islands, resulting in emergence of variants, exemplified by V. cholerae O139 and V. cholerae O1 El Tor hybrid clones. Based on the comparative genomics it is concluded that V. cholerae undergoes extensive genetic recombination via lateral gene transfer, and, therefore, genome assortment, not serogroup, should be used to define pathogenic V. cholerae clones.
ESTHER : Chun_2009_Proc.Natl.Acad.Sci.U.S.A_106_15442
PubMedSearch : Chun_2009_Proc.Natl.Acad.Sci.U.S.A_106_15442
PubMedID: 19720995
Gene_locus related to this paper: vibch-lipas , vibch-VC0135 , vibch-VC1418 , vibch-VC1974 , vibch-VC2432 , vibch-VC2718 , vibch-VCA0063 , vibch-VCA0490 , vibch-VCA0688 , vibch-VCA0754 , vibch-y1892 , vibch-y2276

Title : Complete genome sequence of the ethanol producer Zymomonas mobilis NCIMB 11163 - Kouvelis_2009_J.Bacteriol_191_7140
Author(s) : Kouvelis VN , Saunders E , Brettin TS , Bruce D , Detter C , Han C , Typas MA , Pappas KM
Ref : Journal of Bacteriology , 191 :7140 , 2009
Abstract : Zymomonas mobilis is an ethanol-producing alphaproteobacterium currently considered a major candidate organism for bioethanol production. Here we report the finished and annotated genome sequence of Z. mobilis subsp. mobilis strain NCIMB 11163, a British ale-infecting isolate. This is the first Z. mobilis strain whose genome, chromosomal and plasmid, is presented in its entirety.
ESTHER : Kouvelis_2009_J.Bacteriol_191_7140
PubMedSearch : Kouvelis_2009_J.Bacteriol_191_7140
PubMedID: 19767433
Gene_locus related to this paper: zymmo-DLH , zymmo-GAA , zymmo-q5nmh0 , zymmo-q5nmm8 , zymmo-Q8GF33 , zymmo-Q8GF53

Title : Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina) - Martinez_2008_Nat.Biotechnol_26_553
Author(s) : Martinez D , Berka RM , Henrissat B , Saloheimo M , Arvas M , Baker SE , Chapman J , Chertkov O , Coutinho PM , Cullen D , Danchin EG , Grigoriev IV , Harris P , Jackson M , Kubicek CP , Han CS , Ho I , Larrondo LF , de Leon AL , Magnuson JK , Merino S , Misra M , Nelson B , Putnam N , Robbertse B , Salamov AA , Schmoll M , Terry A , Thayer N , Westerholm-Parvinen A , Schoch CL , Yao J , Barabote R , Nelson MA , Detter C , Bruce D , Kuske CR , Xie G , Richardson P , Rokhsar DS , Lucas SM , Rubin EM , Dunn-Coleman N , Ward M , Brettin TS
Ref : Nat Biotechnol , 26 :553 , 2008
Abstract : Trichoderma reesei is the main industrial source of cellulases and hemicellulases used to depolymerize biomass to simple sugars that are converted to chemical intermediates and biofuels, such as ethanol. We assembled 89 scaffolds (sets of ordered and oriented contigs) to generate 34 Mbp of nearly contiguous T. reesei genome sequence comprising 9,129 predicted gene models. Unexpectedly, considering the industrial utility and effectiveness of the carbohydrate-active enzymes of T. reesei, its genome encodes fewer cellulases and hemicellulases than any other sequenced fungus able to hydrolyze plant cell wall polysaccharides. Many T. reesei genes encoding carbohydrate-active enzymes are distributed nonrandomly in clusters that lie between regions of synteny with other Sordariomycetes. Numerous genes encoding biosynthetic pathways for secondary metabolites may promote survival of T. reesei in its competitive soil habitat, but genome analysis provided little mechanistic insight into its extraordinary capacity for protein secretion. Our analysis, coupled with the genome sequence data, provides a roadmap for constructing enhanced T. reesei strains for industrial applications such as biofuel production.
ESTHER : Martinez_2008_Nat.Biotechnol_26_553
PubMedSearch : Martinez_2008_Nat.Biotechnol_26_553
PubMedID: 18454138
Gene_locus related to this paper: hypjq-g0rh85 , hypjq-cip2 , hypjq-g0r9d1 , hypjq-g0r810 , hypjq-g0rbm4 , hypjq-g0rez4 , hypjq-g0rfr3 , hypjq-g0rg60 , hypjq-g0rij9 , hypjq-g0riu1 , hypjq-g0rl87 , hypjq-g0rlh4 , hypjq-g0rme5 , hypjq-g0rwy5 , hypje-axylest , hypje-q7z9m3 , hypjq-g0r6x2 , hypje-a0a024s1b8 , hypjr-a0a024s1s9 , hypjq-g0rxi5

Title : The complete genome sequence of Bacillus thuringiensis Al Hakam - Challacombe_2007_J.Bacteriol_189_3680
Author(s) : Challacombe JF , Altherr MR , Xie G , Bhotika SS , Brown N , Bruce D , Campbell CS , Campbell ML , Chen J , Chertkov O , Cleland C , Dimitrijevic M , Doggett NA , Fawcett JJ , Glavina T , Goodwin LA , Green LD , Han CS , Hill KK , Hitchcock P , Jackson PJ , Keim P , Kewalramani AR , Longmire J , Lucas S , Malfatti S , Martinez D , McMurry K , Meincke LJ , Misra M , Moseman BL , Mundt M , Munk AC , Okinaka RT , Parson-Quintana B , Reilly LP , Richardson P , Robinson DL , Saunders E , Tapia R , Tesmer JG , Thayer N , Thompson LS , Tice H , Ticknor LO , Wills PL , Gilna P , Brettin TS
Ref : Journal of Bacteriology , 189 :3680 , 2007
Abstract : Bacillus thuringiensis is an insect pathogen that is widely used as a biopesticide (E. Schnepf, N. Crickmore, J. Van Rie, D. Lereclus, J. Baum, J. Feitelson, D. R. Zeigler, and D. H. Dean, Microbiol. Mol. Biol. Rev. 62:775-806, 1998). Here we report the finished, annotated genome sequence of B. thuringiensis Al Hakam, which was collected in Iraq by the United Nations Special Commission (L. Radnedge, P. Agron, K. Hill, P. Jackson, L. Ticknor, P. Keim, and G. Andersen, Appl. Environ. Microbiol. 69:2755-2764, 2003).
ESTHER : Challacombe_2007_J.Bacteriol_189_3680
PubMedSearch : Challacombe_2007_J.Bacteriol_189_3680
PubMedID: 17337577
Gene_locus related to this paper: bacah-a0rcd1 , bacah-a0rer5 , bacah-a0rev7 , bacan-BA1019 , bacan-BA1242 , bacan-BA2392 , bacan-BA2607 , bacan-BA3343 , bacan-BA3863 , bacan-BA3877 , bacan-BA4324 , bacan-BA4338 , bacan-BA4577 , bacan-BA5009 , bacan-BA5110 , bacan-BA5136 , bacan-DHBF , bacc1-q73a27 , bacc1-q73c93 , bacce-BC0192 , bacce-BC1788 , bacce-BC1954 , bacce-BC2141 , bacce-BC2171 , bacce-BC4730 , bacce-BC4862 , bacce-BC5130 , bacce-PHAC , bacce-q72yu1 , baccr-pepx , bachk-q6hcl3 , bachk-q6hgn4 , bachk-q6hgp9 , bachk-q6hig3 , bachk-q6hit8

Title : Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT\/A3, \/Ba4 and \/B1 clusters are located within plasmids - Smith_2007_PLoS.One_2_e1271
Author(s) : Smith TJ , Hill KK , Foley BT , Detter JC , Munk AC , Bruce DC , Doggett NA , Smith LA , Marks JD , Xie G , Brettin TS
Ref : PLoS ONE , 2 :e1271 , 2007
Abstract : BACKGROUND: Clostridium botulinum and related clostridial species express extremely potent neurotoxins known as botulinum neurotoxins (BoNTs) that cause long-lasting, potentially fatal intoxications in humans and other mammals. The amino acid variation within the BoNT is used to categorize the species into seven immunologically distinct BoNT serotypes (A-G) which are further divided into subtypes. The BoNTs are located within two generally conserved gene arrangements known as botulinum progenitor complexes which encode toxin-associated proteins involved in toxin stability and expression. METHODOLOGY/PRINCIPAL FINDINGS: Because serotype A and B strains are responsible for the vast majority of human botulism cases worldwide, the location, arrangement and sequences of genes from eight different toxin complexes representing four different BoNT/A subtypes (BoNT/A1-Ba4) and one BoNT/B1 strain were examined. The bivalent Ba4 strain contained both the BoNT/A4 and BoNT/bvB toxin clusters. The arrangements of the BoNT/A3 and BoNT/A4 subtypes differed from the BoNT/A1 strains and were similar to those of BoNT/A2. However, unlike the BoNT/A2 subtype, the toxin complex genes of BoNT/A3 and BoNT/A4 were found within large plasmids and not within the chromosome. In the Ba4 strain, both BoNT toxin clusters (A4 and bivalent B) were located within the same 270 kb plasmid, separated by 97 kb. Complete genomic sequencing of the BoNT/B1 strain also revealed that its toxin complex genes were located within a 149 kb plasmid and the BoNT/A3 complex is within a 267 kb plasmid. CONCLUSIONS/SIGNIFICANCE: Despite their size differences and the BoNT genes they contain, the three plasmids containing these toxin cluster genes share significant sequence identity. The presence of partial insertion sequence (IS) elements, evidence of recombination/gene duplication events, and the discovery of the BoNT/A3, BoNT/Ba4 and BoNT/B1 toxin complex genes within plasmids illustrate the different mechanisms by which these genes move among diverse genetic backgrounds of C. botulinum.
ESTHER : Smith_2007_PLoS.One_2_e1271
PubMedSearch : Smith_2007_PLoS.One_2_e1271
PubMedID: 18060065
Gene_locus related to this paper: clob1-a7fqm2 , clob1-a7fv94 , clobl-a7gbn0 , clobh-pip , clobh-a5i3m0 , clob1-a7fvd9 , clobl-a7gbt4

Title : Complete genomic characterization of a pathogenic A.II strain of Francisella tularensis subspecies tularensis - Beckstrom-Sternberg_2007_PLoS.One_2_e947
Author(s) : Beckstrom-Sternberg SM , Auerbach RK , Godbole S , Pearson JV , Beckstrom-Sternberg JS , Deng Z , Munk C , Kubota K , Zhou Y , Bruce D , Noronha J , Scheuermann RH , Wang A , Wei X , Wang J , Hao J , Wagner DM , Brettin TS , Brown N , Gilna P , Keim PS
Ref : PLoS ONE , 2 :e947 , 2007
Abstract : Francisella tularensis is the causative agent of tularemia, which is a highly lethal disease from nature and potentially from a biological weapon. This species contains four recognized subspecies including the North American endemic F. tularensis subsp. tularensis (type A), whose genetic diversity is correlated with its geographic distribution including a major population subdivision referred to as A.I and A.II. The biological significance of the A.I - A.II genetic differentiation is unknown, though there are suggestive ecological and epidemiological correlations. In order to understand the differentiation at the genomic level, we have determined the complete sequence of an A.II strain (WY96-3418) and compared it to the genome of Schu S4 from the A.I population. We find that this A.II genome is 1,898,476 bp in size with 1,820 genes, 1,303 of which code for proteins. While extensive genomic variation exists between "WY96" and Schu S4, there is only one whole gene difference. This one gene difference is a hypothetical protein of unknown function. In contrast, there are numerous SNPs (3,367), small indels (1,015), IS element differences (7) and large chromosomal rearrangements (31), including both inversions and translocations. The rearrangement borders are frequently associated with IS elements, which would facilitate intragenomic recombination events. The pathogenicity island duplicated regions (DR1 and DR2) are essentially identical in WY96 but vary relative to Schu S4 at 60 nucleotide positions. Other potential virulence-associated genes (231) varied at 559 nucleotide positions, including 357 non-synonymous changes. Molecular clock estimates for the divergence time between A.I and A.II genomes for different chromosomal regions ranged from 866 to 2131 years before present. This paper is the first complete genomic characterization of a member of the A.II clade of Francisella tularensis subsp. tularensis.
ESTHER : Beckstrom-Sternberg_2007_PLoS.One_2_e947
PubMedSearch : Beckstrom-Sternberg_2007_PLoS.One_2_e947
PubMedID: 17895988
Gene_locus related to this paper: fratt-q5ngu5

Title : Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii - Xie_2007_Appl.Environ.Microbiol_73_3536
Author(s) : Xie G , Bruce DC , Challacombe JF , Chertkov O , Detter JC , Gilna P , Han CS , Lucas S , Misra M , Myers GL , Richardson P , Tapia R , Thayer N , Thompson LS , Brettin TS , Henrissat B , Wilson DB , McBride MJ
Ref : Applied Environmental Microbiology , 73 :3536 , 2007
Abstract : The complete DNA sequence of the aerobic cellulolytic soil bacterium Cytophaga hutchinsonii, which belongs to the phylum Bacteroidetes, is presented. The genome consists of a single, circular, 4.43-Mb chromosome containing 3,790 open reading frames, 1,986 of which have been assigned a tentative function. Two of the most striking characteristics of C. hutchinsonii are its rapid gliding motility over surfaces and its contact-dependent digestion of crystalline cellulose. The mechanism of C. hutchinsonii motility is not known, but its genome contains homologs for each of the gld genes that are required for gliding of the distantly related bacteroidete Flavobacterium johnsoniae. Cytophaga-Flavobacterium gliding appears to be novel and does not involve well-studied motility organelles such as flagella or type IV pili. Many genes thought to encode proteins involved in cellulose utilization were identified. These include candidate endo-beta-1,4-glucanases and beta-glucosidases. Surprisingly, obvious homologs of known cellobiohydrolases were not detected. Since such enzymes are needed for efficient cellulose digestion by well-studied cellulolytic bacteria, C. hutchinsonii either has novel cellobiohydrolases or has an unusual method of cellulose utilization. Genes encoding proteins with cohesin domains, which are characteristic of cellulosomes, were absent, but many proteins predicted to be involved in polysaccharide utilization had putative D5 domains, which are thought to be involved in anchoring proteins to the cell surface.
ESTHER : Xie_2007_Appl.Environ.Microbiol_73_3536
PubMedSearch : Xie_2007_Appl.Environ.Microbiol_73_3536
PubMedID: 17400776
Gene_locus related to this paper: cyth3-q11pu3 , cyth3-q11rb1 , cyth3-q11sp5 , cyth3-q11sp6 , cyth3-q11ty5 , cyth3-q11vh6 , cyth3-q11vj5 , cyth3-q11w17 , cyth3-q11xy0

Title : Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd - Challacombe_2007_J.Bacteriol_189_1890
Author(s) : Challacombe JF , Duncan AJ , Brettin TS , Bruce D , Chertkov O , Detter JC , Han CS , Misra M , Richardson P , Tapia R , Thayer N , Xie G , Inzana TJ
Ref : Journal of Bacteriology , 189 :1890 , 2007
Abstract : Haemophilus somnus can be either a commensal of bovine mucosal surfaces or an opportunistic pathogen. Pathogenic strains of H. somnus are a significant cause of systemic disease in cattle. We report the genome sequence of H. somnus 129Pt, a nonpathogenic commensal preputial isolate, and the results of a genome-wide comparative analysis of H. somnus 129Pt, Haemophilus influenzae Rd, and Haemophilus ducreyi 35000HP. We found unique genes in H. somnus 129Pt involved in lipooligosaccharide biosynthesis, carbohydrate uptake and metabolism, cation transport, amino acid metabolism, ubiquinone and menaquinone biosynthesis, cell surface adhesion, biosynthesis of cofactors, energy metabolism, and electron transport. There were also many genes in common among the three organisms. Our comparative analyses of H. somnus 129Pt, H. influenzae Rd, and H. ducreyi 35000HP revealed similarities and differences in the numbers and compositions of genes involved in metabolism, host colonization, and persistence. These results lay a foundation for research on the host specificities and niche preferences of these organisms. Future comparisons between H. somnus 129Pt and virulent strains will aid in the development of protective strategies and vaccines to protect cattle against H. somnus disease.
ESTHER : Challacombe_2007_J.Bacteriol_189_1890
PubMedSearch : Challacombe_2007_J.Bacteriol_189_1890
PubMedID: 17172329
Gene_locus related to this paper: haes1-q0i317 , haes1-q0i470 , haes2-b0utw7 , haes2-b0uvn0 , haes2-b0uwc2

Title : Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis - Han_2006_J.Bacteriol_188_3382
Author(s) : Han CS , Xie G , Challacombe JF , Altherr MR , Bhotika SS , Brown N , Bruce D , Campbell CS , Campbell ML , Chen J , Chertkov O , Cleland C , Dimitrijevic M , Doggett NA , Fawcett JJ , Glavina T , Goodwin LA , Green LD , Hill KK , Hitchcock P , Jackson PJ , Keim P , Kewalramani AR , Longmire J , Lucas S , Malfatti S , McMurry K , Meincke LJ , Misra M , Moseman BL , Mundt M , Munk AC , Okinaka RT , Parson-Quintana B , Reilly LP , Richardson P , Robinson DL , Rubin E , Saunders E , Tapia R , Tesmer JG , Thayer N , Thompson LS , Tice H , Ticknor LO , Wills PL , Brettin TS , Gilna P
Ref : Journal of Bacteriology , 188 :3382 , 2006
Abstract : Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of B. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.
ESTHER : Han_2006_J.Bacteriol_188_3382
PubMedSearch : Han_2006_J.Bacteriol_188_3382
PubMedID: 16621833
Gene_locus related to this paper: bacan-BA0954 , bacan-BA2607 , bacce-BC0968 , bacce-BC3133 , bacce-BC5130 , bacce-c2mr40 , baccz-q63gk2

Title : The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes - Maeder_2006_J.Bacteriol_188_7922
Author(s) : Maeder DL , Anderson I , Brettin TS , Bruce DC , Gilna P , Han CS , Lapidus A , Metcalf WW , Saunders E , Tapia R , Sowers KR
Ref : Journal of Bacteriology , 188 :7922 , 2006
Abstract : We report here a comparative analysis of the genome sequence of Methanosarcina barkeri with those of Methanosarcina acetivorans and Methanosarcina mazei. The genome of M. barkeri is distinguished by having an organization that is well conserved with respect to the other Methanosarcina spp. in the region proximal to the origin of replication, with interspecies gene similarities as high as 95%. However, it is disordered and marked by increased transposase frequency and decreased gene synteny and gene density in the distal semigenome. Of the 3,680 open reading frames (ORFs) in M. barkeri, 746 had homologs with better than 80% identity to both M. acetivorans and M. mazei, while 128 nonhypothetical ORFs were unique (nonorthologous) among these species, including a complete formate dehydrogenase operon, genes required for N-acetylmuramic acid synthesis, a 14-gene gas vesicle cluster, and a bacterial-like P450-specific ferredoxin reductase cluster not previously observed or characterized for this genus. A cryptic 36-kbp plasmid sequence that contains an orc1 gene flanked by a presumptive origin of replication consisting of 38 tandem repeats of a 143-nucleotide motif was detected in M. barkeri. Three-way comparison of these genomes reveals differing mechanisms for the accrual of changes. Elongation of the relatively large M. acetivorans genome is the result of uniformly distributed multiple gene scale insertions and duplications, while the M. barkeri genome is characterized by localized inversions associated with the loss of gene content. In contrast, the short M. mazei genome most closely approximates the putative ancestral organizational state of these species.
ESTHER : Maeder_2006_J.Bacteriol_188_7922
PubMedSearch : Maeder_2006_J.Bacteriol_188_7922
PubMedID: 16980466
Gene_locus related to this paper: metbf-q46ca9 , metbf-q469u5