(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Ascomycota: NE > saccharomyceta: NE > Pezizomycotina: NE > leotiomyceta: NE > sordariomyceta: NE > Sordariomycetes: NE > Sordariomycetidae: NE > Sordariales: NE > Sordariaceae: NE > Neurospora: NE > Neurospora crassa: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Neurospora crassa OR74A: N, E.
Sordaria macrospora k-hell: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MSQETDSTVSTTSIVFDRIQERLDTKEFPARGTDGDDNDSLKDELNNDDL ETGPFLGNASPSSRSNQRSSADGQRMDRSLRRWLFIVSGALVATWVIGLI FFVSSKAYKPSSSFAHDPQATVTHGSGKKVTLDQVLNNEWRAKSHSISWI AGVNGEDGLLLEKEGANKDYLVVEDVRAQNPSSVEASKSKTLIKDKLFEF ANKTYWPTVTVPSRDLKKVLLATDVQNNWRHSYYAVYWIFDVETQQAEPL VPYDADARLQLASWSPTSDAIVYTRDNNMFLRKLDSDKIVQITRDGSADV FNGVPDWVYEEEVLASGVATWWSEDGNYVAFLRTNETGVPEYPIQYFVSR PSGEEPKPGEENYPEVRQIKYPKAGAHNPIVDLKFYDVKRGDVFSVDISG RFADDDRLITEVIWAGKQVLIKETNRVSDVMRVVLVDVGSRTGKAVRTVD VNDIDGGWFEISHKTKFIPADPANGRPDDGYVDTIIHNNGDHLAYFTPLD NPNPIMLTSGDYEVVDAPSAVDLQRNLVYFVSTKESSIQRHVYQVKLTGE DMTPVTDTSKEGYYAISFSTGAGYALVSYQGPNIPWQKVISTPSNPDKYE HVVEENKDLAEAAKKHELPINIYGTINVDGVELNYIERRPPHFDKNKKYP VLFQQYSGPVSQTVKKTFAVDFQSFVAAGLGYICVTVDGRGTGFIGRKNR VIIRGNLGTWESHDQIAAAKHWAQKDYIDEDRLAIWGWSYGGYMTLKTLE QDAGQTFKYGMAVAPVTDWRFYDSIYTERYMRTPQTNPEGYESAAVTNVT ALSQNVRFLLMHGVADDNVHMQNSLTLLDALDQRSVENYDVQVFPDSDHG IYFHNANRIVFDKLTNWLVNAFNGEWLKIANAQPNGMKRRALPTA
Filamentous fungi are of great importance in ecology, agriculture, medicine, and biotechnology. Thus, it is not surprising that genomes for more than 100 filamentous fungi have been sequenced, most of them by Sanger sequencing. While next-generation sequencing techniques have revolutionized genome resequencing, e.g. for strain comparisons, genetic mapping, or transcriptome and ChIP analyses, de novo assembly of eukaryotic genomes still presents significant hurdles, because of their large size and stretches of repetitive sequences. Filamentous fungi contain few repetitive regions in their 30-90 Mb genomes and thus are suitable candidates to test de novo genome assembly from short sequence reads. Here, we present a high-quality draft sequence of the Sordaria macrospora genome that was obtained by a combination of Illumina/Solexa and Roche/454 sequencing. Paired-end Solexa sequencing of genomic DNA to 85-fold coverage and an additional 10-fold coverage by single-end 454 sequencing resulted in approximately 4 Gb of DNA sequence. Reads were assembled to a 40 Mb draft version (N50 of 117 kb) with the Velvet assembler. Comparative analysis with Neurospora genomes increased the N50 to 498 kb. The S. macrospora genome contains even fewer repeat regions than its closest sequenced relative, Neurospora crassa. Comparison with genomes of other fungi showed that S. macrospora, a model organism for morphogenesis and meiosis, harbors duplications of several genes involved in self/nonself-recognition. Furthermore, S. macrospora contains more polyketide biosynthesis genes than N. crassa. Phylogenetic analyses suggest that some of these genes may have been acquired by horizontal gene transfer from a distantly related ascomycete group. Our study shows that, for typical filamentous fungi, de novo assembly of genomes from short sequence reads alone is feasible, that a mixture of Solexa and 454 sequencing substantially improves the assembly, and that the resulting data can be used for comparative studies to address basic questions of fungal biology.
Neurospora crassa is a central organism in the history of twentieth-century genetics, biochemistry and molecular biology. Here, we report a high-quality draft sequence of the N. crassa genome. The approximately 40-megabase genome encodes about 10,000 protein-coding genes--more than twice as many as in the fission yeast Schizosaccharomyces pombe and only about 25% fewer than in the fruitfly Drosophila melanogaster. Analysis of the gene set yields insights into unexpected aspects of Neurospora biology including the identification of genes potentially associated with red light photobiology, genes implicated in secondary metabolism, and important differences in Ca2+ signalling as compared with plants and animals. Neurospora possesses the widest array of genome defence mechanisms known for any eukaryotic organism, including a process unique to fungi called repeat-induced point mutation (RIP). Genome analysis suggests that RIP has had a profound impact on genome evolution, greatly slowing the creation of new genes through genomic duplication and resulting in a genome with an unusually low proportion of closely related genes.
