Lee YH

References (42)

Title : SGLT2 and DPP4 inhibitors improve Alzheimer's disease-like pathology and cognitive function through distinct mechanisms in a T2D-AD mouse model - Sim_2023_Biomed.Pharmacother_168_115755
Author(s) : Sim AY , Choi DH , Kim JY , Kim ER , Goh AR , Lee YH , Lee JE
Ref : Biomed Pharmacother , 168 :115755 , 2023
Abstract : Alzheimer's disease (AD) and type 2 diabetes mellitus (T2D) share common features, including insulin resistance. Brain insulin resistance has been implicated as a key factor in the pathogenesis of AD. Recent studies have demonstrated that anti-diabetic drugs sodium-glucose cotransporter-2 inhibitor (SGLT2-i) and dipeptidyl peptidase-4 inhibitor (DPP4-i) improve insulin sensitivity and provide neuroprotection. However, the effects of these two inhibitors on the brain metabolism and insulin resistance remain uninvestigated. We developed a T2D-AD mouse model using a high-fat diet (HFD) for 19 weeks along with a single dose of streptozotocin (100 mg/kg, intraperitoneally) at the fourth week of HFD initiation. Subsequently, the animals were treated with SGLT2-i (empagliflozin, 25 mg/kg/day orally [p.o.]) and DPP4-i (sitagliptin, 100 mg/kg/day p.o.) for 7 weeks. Subsequently, behavioral tests were performed, and the expression of insulin signaling, AD-related, and other signaling pathway proteins in the brain were examined. T2D-AD mice not only showed increased blood glucose levels and body weight but also insulin resistance. SGLT2-i and DPP4-i effectively ameliorated insulin sensitivity and reduced body weight in these mice. Furthermore, SGLT2-i and DPP4-i significantly improved hippocampal-dependent learning, memory, and cognitive functions in the T2D-AD mouse model. Interestingly, SGLT2-i and DPP4-i reduced the hyperphosphorylated tau (pTau) levels and amyloid beta (Abeta) accumulation and enhanced brain insulin signaling. SGLT2-i reduced pTau accumulation through the angiotensin converting enzyme-2/angiotensin (1-7)/ mitochondrial assembly receptor axis, whereas DPP4-i reduced Abeta accumulation by increasing insulin-degrading enzyme levels. These findings suggest that SGLT2-i and DPP4-i prevent AD-like pathology and cognitive dysfunction in T2D mice potentially through affecting brain insulin signaling via different mechanisms.
ESTHER : Sim_2023_Biomed.Pharmacother_168_115755
PubMedSearch : Sim_2023_Biomed.Pharmacother_168_115755
PubMedID: 37871560

Title : Designing multi-target-directed flavonoids: a strategic approach to Alzheimer's disease - Park_2023_Chem.Sci_14_9293
Author(s) : Park S , Kim M , Lin Y , Hong M , Nam G , Mieczkowski A , Kardos J , Lee YH , Lim MH
Ref : Chem Sci , 14 :9293 , 2023
Abstract : The underlying causes of Alzheimer's disease (AD) remain a mystery, with multiple pathological components, including oxidative stress, acetylcholinesterase, amyloid-beta, and metal ions, all playing a role. Here we report a strategic approach to designing flavonoids that can effectively tackle multiple pathological elements involved in AD. Our systematic investigations revealed key structural features for flavonoids to simultaneously target and regulate pathogenic targets. Our findings led to the development of a highly promising flavonoid that exhibits a range of functions, based on a complete structure-activity relationship analysis. Furthermore, our mechanistic studies confirmed that this flavonoid's versatile reactivities are driven by its redox potential and direct interactions with pathogenic factors. This work highlights the potential of multi-target-directed flavonoids as a novel solution in the fight against AD.
ESTHER : Park_2023_Chem.Sci_14_9293
PubMedSearch : Park_2023_Chem.Sci_14_9293
PubMedID: 37712013

Title : Epoxyeicosatrienoic acids and soluble epoxide hydrolase in physiology and diseases of the central nervous system - Kuo_2022_Chin.J.Physiol_65_1
Author(s) : Kuo YM , Lee YH
Ref : Chin Journal de Physiologie , 65 :1 , 2022
Abstract : Epoxyeicosatrienoic acids (EETs) are fatty acid signaling molecules synthesized by cytochrome P450 epoxygenases from arachidonic acid. The biological activity of EETs is terminated when being metabolized by soluble epoxide hydrolase (sEH), a process that serves as a key regulator of tissue EETs levels. EETs act through several signaling pathways to mediate various beneficial effects, including anti-inflammation, anti-apoptosis, and anti-oxidation with relieve of endoplasmic reticulum stress, thereby sEH has become a potential therapeutic target in cardiovascular disease and cancer therapy. Enzymes for EET biosynthesis and metabolism are both widely detected in both neuron and glial cells in the central nervous system (CNS). Recent studies discovered that astrocyte-derived EETs not only mediate neurovascular coupling and neuronal excitability by maintaining glutamate homeostasis but also glia-dependent neuroprotection. Genetic ablation as well as pharmacologic inhibition of sEH has greatly helped to elucidate the physiologic actions of EETs, and maintaining or elevating brain EETs level has been demonstrated beneficial effects in CNS disease models. Here, we review the literature regarding the studies on the bioactivity of EETs and their metabolic enzyme sEH with special attention paid to their action mechanisms in the CNS, including their modulation of neuronal activity, attenuation of neuroinflammation, regulation of cerebral blood flow, and improvement of neuronal and glial cells survival. We further reviewed the recent advance on the potential application of sEH inhibition for treating cerebrovascular disease, epilepsy, and pain disorder.
ESTHER : Kuo_2022_Chin.J.Physiol_65_1
PubMedSearch : Kuo_2022_Chin.J.Physiol_65_1
PubMedID: 35229747

Title : Role of DPP-4 and SGLT2 Inhibitors Connected to Alzheimer Disease in Type 2 Diabetes Mellitus - Sim_2021_Front.Neurosci_15_708547
Author(s) : Sim AY , Barua S , Kim JY , Lee YH , Lee JE
Ref : Front Neurosci , 15 :708547 , 2021
Abstract : Alzheimer's disease (AD) is characterized by memory loss and cognitive decline. Additionally, abnormal extracellular amyloid plaques accumulation and nerve damage caused by intracellular neurofibrillary tangles, and tau protein are characteristic of AD. Furthermore, AD is associated with oxidative stress, impaired mitochondrial structure and function, denormalization, and inflammatory responses. Recently, besides the amyloid beta hypothesis, another hypothesis linking AD to systemic diseases has been put forth by multiple studies as a probable cause for AD. Particularly, type 2 diabetes mellitus (T2DM) and its features, including hyperinsulinemia, and chronic hyperglycemia with an inflammatory response, have been shown to be closely related to AD through insulin resistance. The brain cannot synthesize or store glucose, but it does require glucose, and the use of glucose in the brain is higher than that in any other organ in the mammalian body. One of the therapeutic drugs for T2DM, dipeptidyl peptidase-4 (DPP-4) inhibitor, suppresses the degradation of incretins, glucagon-like peptides and glucose-dependent insulinotropic peptide. Sodium-glucose cotransporter 2 (SGLT2) inhibitors, recently used in T2DM treatment, have a unique mechanism of action via inhibition of renal glucose reabsorption, and which is different from the mechanisms of previously used medications. This manuscript reviews the pathophysiological relationship between the two diseases, AD and T2DM, and the pharmacological effects of therapeutic T2DM drugs, especially DPP-4 inhibitors, and SGLT2 inhibitors.
ESTHER : Sim_2021_Front.Neurosci_15_708547
PubMedSearch : Sim_2021_Front.Neurosci_15_708547
PubMedID: 34489627

Title : Soluble epoxide hydrolase modulates immune responses in activated astrocytes involving regulation of STAT3 activity - Hung_2019_J.Neuroinflammation_16_123
Author(s) : Hung CC , Lee YH , Kuo YM , Hsu PC , Tsay HJ , Hsu YT , Lee CC , Liang JJ , Shie FS
Ref : J Neuroinflammation , 16 :123 , 2019
Abstract : BACKGROUND: Astrocyte activation is a common pathological feature in many brain diseases with neuroinflammation, and revealing the underlying mechanisms might shed light on the regulatory processes of the diseases. Recently, soluble epoxide hydrolase (sEH) has been proposed to affect neuroinflammation in brain injuries. However, the roles of astrocytic sEH in brains with neurodegeneration remain unclear. METHODS: The expression of astrocytic sEH in the brains of APPswe/PSEN1dE9 (APP/PS1) mice developing Alzheimer's disease (AD)-like pathology was evaluated by confocal imaging. LPS-activated primary astrocytes with mRNA silencing or overexpression of sEH were used to investigate its regulatory roles in astrocyte activation and the induction of pro-inflammatory markers. Primary astrocytes isolated from a sEH knockout (sEH(-/-)) background were also applied. RESULTS: The immunoreactivity of sEH was increased in activated astrocytes in parallel with the progression of AD in APP/PS1 mice. Our data from primary astrocyte cultures further demonstrate that the overexpression of sEH ameliorated, while the silencing of sEH mRNA enhanced, the lipopolysaccharides (LPS)-induced expression of pro-inflammatory markers, such as inducible nitric oxide, cyclooxygenase 2 (COX-2), and pro-inflammatory cytokines. These findings suggest that sEH negatively regulates astrocyte immune responses. Enhanced immune responses found in LPS-activated sEH(-/-) astrocytes also support the notion that the expression of sEH could suppress the immune responses during astrocyte activation. Similarly, sEH(-/-) mice that received intraperitoneal injection of LPS showed exacerbated astrocyte activation in the brain, as observed by the elevated expression of glial fibrillary acidic protein (GFAP) and pro-inflammatory markers. Moreover, our data show that the phosphorylation of the signal transducer and activator of transcription 3 (STAT3) was upregulated in activated astrocytes from sEH mouse brains, and the pharmacological blockade of STAT3 activity alleviated the pro-inflammatory effects of sEH deletion in LPS-activated primary astrocytes. CONCLUSIONS: Our results provide evidence, for the first time, showing that sEH negatively regulates astrocytic immune responses and GFAP expression, while the underlying mechanism at least partly involves the downregulation of STAT3 phosphorylation. The discovery of a novel function for sEH in the negative control of astrocytic immune responses involving STAT3 activation confers further insights into the regulatory machinery of astrocyte activation during the development of neurodegeneration.
ESTHER : Hung_2019_J.Neuroinflammation_16_123
PubMedSearch : Hung_2019_J.Neuroinflammation_16_123
PubMedID: 31176371

Title : Genome Sequence of Striga asiatica Provides Insight into the Evolution of Plant Parasitism - Yoshida_2019_Curr.Biol_29_3041
Author(s) : Yoshida S , Kim S , Wafula EK , Tanskanen J , Kim YM , Honaas L , Yang Z , Spallek T , Conn CE , Ichihashi Y , Cheong K , Cui S , Der JP , Gundlach H , Jiao Y , Hori C , Ishida JK , Kasahara H , Kiba T , Kim MS , Koo N , Laohavisit A , Lee YH , Lumba S , McCourt P , Mortimer JC , Mutuku JM , Nomura T , Sasaki-Sekimoto Y , Seto Y , Wang Y , Wakatake T , Sakakibara H , Demura T , Yamaguchi S , Yoneyama K , Manabe RI , Nelson DC , Schulman AH , Timko MP , dePamphilis CW , Choi D , Shirasu K
Ref : Current Biology , 29 :3041 , 2019
Abstract : Parasitic plants in the genus Striga, commonly known as witchweeds, cause major crop losses in sub-Saharan Africa and pose a threat to agriculture worldwide. An understanding of Striga parasite biology, which could lead to agricultural solutions, has been hampered by the lack of genome information. Here, we report the draft genome sequence of Striga asiatica with 34,577 predicted protein-coding genes, which reflects gene family contractions and expansions that are consistent with a three-phase model of parasitic plant genome evolution. Striga seeds germinate in response to host-derived strigolactones (SLs) and then develop a specialized penetration structure, the haustorium, to invade the host root. A family of SL receptors has undergone a striking expansion, suggesting a molecular basis for the evolution of broad host range among Striga spp. We found that genes involved in lateral root development in non-parasitic model species are coordinately induced during haustorium development in Striga, suggesting a pathway that was partly co-opted during the evolution of the haustorium. In addition, we found evidence for horizontal transfer of host genes as well as retrotransposons, indicating gene flow to S. asiatica from hosts. Our results provide valuable insights into the evolution of parasitism and a key resource for the future development of Striga control strategies.
ESTHER : Yoshida_2019_Curr.Biol_29_3041
PubMedSearch : Yoshida_2019_Curr.Biol_29_3041
PubMedID: 31522940
Gene_locus related to this paper: straf-a0a5a7qxe3

Title : Soluble Epoxide Hydrolase Inhibition Attenuates Excitotoxicity Involving 14,15-Epoxyeicosatrienoic Acid-Mediated Astrocytic Survival and Plasticity to Preserve Glutamate Homeostasis - Kuo_2019_Mol.Neurobiol_56_8451
Author(s) : Kuo YM , Hsu PC , Hung CC , Hu YY , Huang YJ , Gan YL , Lin CH , Shie FS , Chang WK , Kao LS , Tsou MY , Lee YH
Ref : Molecular Neurobiology , 56 :8451 , 2019
Abstract : Astrocytes play pivotal roles in regulating glutamate homeostasis at tripartite synapses. Inhibition of soluble epoxide hydrolase (sEHi) provides neuroprotection by blocking the degradation of 14,15-epoxyeicosatrienoic acid (14,15-EET), a lipid mediator whose synthesis can be activated downstream from group 1 metabotropic glutamate receptor (mGluR) signaling in astrocytes. However, it is unclear how sEHi regulates glutamate excitotoxicity. Here, we used three primary rat cortical culture systems, neuron-enriched (NE), astrocyte-enriched glia-neuron mix (GN), and purified astrocytes, to delineate the underlying mechanism by which sEHi and 14,15-EET attenuate excitotoxicity. We found that sEH inhibitor 12-(3-adamantan-1-yl-ureido)-dodecanoic acid (AUDA) and 14,15-EET both attenuated N-methyl-D-aspartate (NMDA)-induced neurite damage and cell death in GN, not NE, cortical cultures. The anti-excitotoxic effects of 14,15-EET and AUDA were both blocked by the group 1 mGluR5 antagonist 2-methyl-6-(phenylethynyl)pyridine (MPEP), as were their protective effects against NMDA-disrupted perineuronal astrocyte processes expressing glutamate transporter-1 (GLT-1) and subsequent glutamate uptake. Knockdown of sEH expression also attenuated NMDA neurotoxicity in mGluR5- and GLT-1-dependent manners. The 14,15-EET/AUDA-preserved astroglial integrity was confirmed in glutamate-stimulated primary astrocytes along with the reduction of the c-Jun N-terminal kinase 1 phosphorylation, in which the 14,15-EET effect is mGluR5-dependent. In vivo studies validated that sEHi and genetic deletion of sEH (Ephx2-KO) ameliorated excitotoxic kainic acid-induced seizure, memory impairment, and neuronal loss while preserving GLT-1-expressing perineuronal astrocytes in hippocampal CA3 subregions. These results suggest that 14,15-EET mediates mGluR5-dependent anti-excitotoxicity by protecting astrocytes to maintain glutamate homeostasis, which may account for the beneficial effect of sEH inhibition in excitotoxic brain injury and diseases.
ESTHER : Kuo_2019_Mol.Neurobiol_56_8451
PubMedSearch : Kuo_2019_Mol.Neurobiol_56_8451
PubMedID: 31257558

Title : Orobol: An Isoflavone Exhibiting Regulatory Multifunctionality against Four Pathological Features of Alzheimer's Disease - Nam_2019_ACS.Chem.Neurosci_10_3386
Author(s) : Nam G , Ji Y , Lee HJ , Kang J , Yi Y , Kim M , Lin Y , Lee YH , Lim MH
Ref : ACS Chem Neurosci , 10 :3386 , 2019
Abstract : We report orobol as a multifunctional isoflavone with the ability to (i) modulate the aggregation pathways of both metal-free and metal-bound amyloid-beta, (ii) interact with metal ions, (iii) scavenge free radicals, and (iv) inhibit the activity of acetylcholinesterase. Such a framework with multifunctionality could be useful for developing chemical reagents to advance our understanding of multifaceted pathologies of neurodegenerative disorders, including Alzheimer's disease.
ESTHER : Nam_2019_ACS.Chem.Neurosci_10_3386
PubMedSearch : Nam_2019_ACS.Chem.Neurosci_10_3386
PubMedID: 31199606

Title : Simultaneous Time-concentration Analysis of Soman and VX Adducts to Butyrylcholinesterase and Albumin by LC-MS-MS - Lee_2018_J.Anal.Toxicol_42_293
Author(s) : Lee JY , Kim C , Lee YH
Ref : J Anal Toxicol , 42 :293 , 2018
Abstract : A sensitive method for the purification and determination of two protein adducts, organophosphorus (OP)-BChE and OP-albumin adducts, in a single sample using a simultaneous sample preparation method was developed and validated using liquid chromatography-tandem mass spectrometry. First, we isolated O-ethyl S-2-diisopropylaminoethyl methyl phosphonothiolate (VX) and O-pinacolyl methylphosphonofluoridate (soman, GD)-BChE adducts using an immunomagnetic separation (IMS) method and the HiTrap Blue affinity column was subsequently used to isolate and purify VX and GD-albumin adducts from the plasma of rhesus monkeys exposed to nerve agents. Additionally, we examined the time-concentration profiles of two biomarkers, VX and GD-nonapeptides and VX and GD-tyrosines, derived from OP-BChE and OP-albumin adducts up to 8 weeks after exposure. Based on the results, we determined that VX and GD-tyrosine is more suitable than VX and GD-nonapeptide as a biomarker owing to its longevity. This integrated approach is expected to be applicable for the quantification of other OP-BChE and OP-albumin adducts in human plasma, thus serving as a potential generic assay for exposure to nerve agents.
ESTHER : Lee_2018_J.Anal.Toxicol_42_293
PubMedSearch : Lee_2018_J.Anal.Toxicol_42_293
PubMedID: 29618078

Title : Blockade of soluble epoxide hydrolase attenuates post-ischemic neuronal hyperexcitation and confers resilience against stroke with TrkB activation - Chang_2018_Sci.Rep_8_118
Author(s) : Chang LH , Lin HC , Huang SS , Chen IC , Chu KW , Chih CL , Liang YW , Lee YC , Chen YY , Lee YH , Lee IH
Ref : Sci Rep , 8 :118 , 2018
Abstract : Inhibition and deletion of soluble epoxide hydrolase (sEH) has been suggested to ameliorate infarction in experimental ischemic stroke possibly via vasoactive epoxyeicosatrienoic acids. However, it is unknown whether the neuroprotective mechanisms involve alteration of post-ischemic neuronal transmission and neurotrophic signaling. We used a permanent middle cerebral artery occlusion (MCAO) model in adult wild-type mice with the sEH inhibitor 12-(3-adamantan-1-yl-ureido)dodecanoic acid (AUDA) post-treatment and in sEH knockout (sEH KO) mice. We found that sensorimotor recovery was significantly enhanced after MCAO in both AUDA-treated and sEH KO mice, with decreased sEH activity and brain infarction. Decreased post-ischemic long-term potentiation (iLTP) was observed in an ex vivo hippocampal oxygen-glucose deprivation model. Tropomyosin receptor kinase B (TrkB) activation, rather than glutamate receptor alteration, was consistently found after the different manipulations. Immunohistochemistry further revealed peri-infarct neuronal TrkB activation and microvasculature augmentation in AUDA-treated and sEH KO mice, suggesting parallel neurovascular enhancement. Mechanistically, pretreatment with a selective TrkB antagonist ANA12 countered the effect of iLTP attenuation induced by sEH deletion ex vivo and abolished the infarct reduction in vivo. Together, the neuroprotective effects of sEH inhibition and gene deletion can both be mediated partially via enhancement of TrkB signaling which attenuated post-ischemic neuroexcitation and neurological deficits.
ESTHER : Chang_2018_Sci.Rep_8_118
PubMedSearch : Chang_2018_Sci.Rep_8_118
PubMedID: 29311641

Title : Soluble Epoxide Hydrolase Inhibition Attenuates MPTP-Induced Neurotoxicity in the Nigrostriatal Dopaminergic System: Involvement of alpha-Synuclein Aggregation and ER Stress - Huang_2018_Mol.Neurobiol_55_138
Author(s) : Huang HJ , Wang YT , Lin HC , Lee YH , Lin AM
Ref : Molecular Neurobiology , 55 :138 , 2018
Abstract : Soluble epoxide hydrolase (sEH) is widely expressed in the mammalian brain and possesses dual enzymatic activities, including C-terminal epoxide hydrolase (C-EH) which degrades epoxyeicosatrienoic acid (EET), a beneficial arachidonic acid metabolite. In the present study, the neuroprotective effect of sEH inhibition on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurodegeneration of nigrostriatal dopaminergic system was investigated using genetic and pharmacological approaches. MPTP (15 mg/kg) was intraperitoneally injected in sEH knockout (KO) mice and C57BL/6J mice as wild-type (WT) mice. Compared with the MPTP-treated WT mice, MPTP-induced reductions in striatal dopamine content and nigral tyrosine hydroxylase level (TH, a biomarker of dopaminergic neurons) were less significant in the treated sEH mice. Furthermore, MPTP-induced HO-1 elevation (a redox-regulated protein), alpha-synuclein aggregation, and caspase 12 activation (a hallmark of ER stress) were less prominent in sEH KO mice than in WT mice. These data indicate that sEH KO mice are more resistant to MPTP-induced neurotoxicity. The pharmacological effect of N-[1-(1-oxopropyl)-4-piperidinyl]-N0-[4-(trifluoromethoxy)phenyl)-urea (TPPU, an sEH inhibitor) on MPTP-induced neurotoxicity was investigated in WT mice. TPPU (1 mg/kg, i.p.) attenuated MPTP-induced reduction in striatal dopamine content, TH-positive cell numbers, TH, and pro-caspase 9 protein levels (an initiator caspase of apoptosis) in mouse SN. Moreover, TPPU reduced MPTP-induced HO-1 elevation, alpha-synuclein aggregation and caspase 12 activation, indicating that TPPU is effective in attenuating MPTP-induced oxidative stress, apoptosis, protein aggregation, and ER stress. In conclusion, our study suggests that sEH is a potential target for developing therapies for parkinsonism. Furthermore, sEH inhibitors may be of clinical significance for treating CNS neurodegenerative diseases.
ESTHER : Huang_2018_Mol.Neurobiol_55_138
PubMedSearch : Huang_2018_Mol.Neurobiol_55_138
PubMedID: 28822080

Title : The association of alcohol consumption with patient survival after organophosphate poisoning: a multicenter retrospective study - Lee_2017_Intern.Emerg.Med_12_519
Author(s) : Lee YH , Oh YT , Lee WW , Ahn HC , Sohn YD , Ahn JY , Min YH , Kim H , Lim SW , Lee KJ , Shin DH , Park SO , Park SM
Ref : Intern Emerg Med , 12 :519 , 2017
Abstract : Organophosphate (OP) intoxication remains a serious worldwide health concern, and many patients with acute OP intoxication have also consumed alcohol. Therefore, we evaluated the association of blood alcohol concentration (BAC) with mortality among patients with OP intoxication. We retrospectively reviewed records from 135 patients who were admitted to an emergency department (ED) for OP intoxication between January 2000 and December 2012. Factors that were associated with patient survival were identified via receiver operating characteristic curve, multiple logistic regression, and Kaplan-Meier survival analyses. Among 135 patients with acute OP poisoning, 112 patients survived (overall mortality rate: 17 %). The non-survivors also exhibited a significantly higher BAC, compared to the survivors [non-survivors: 192 mg/dL, interquartile range (IQR) 97-263 mg/dL vs. survivors: 80 mg/dL, IQR 0-166.75 mg/dL; p < 0.001]. A BAC cut-off value of 173 mg/dL provided an area under the curve of 0.744 [95 % confidence interval (CI) 0.661-0.815], a sensitivity of 65.2 %, and a specificity of 81.2 %. A BAC of >173 mg/dL was associated with a significantly increased risk of 6-month mortality in the multiple logistic regression model (odds ratio 4.92, 95 % CI 1.45-16.67, p = 0.001). The Cox proportional hazard model revealed that a BAC of >173 mg/dL provided a hazard ratio of 3.07 (95 % CI 1.19-7.96, p = 0.021). A BAC of >173 mg/dL is a risk factor for mortality among patients with OP intoxication.
ESTHER : Lee_2017_Intern.Emerg.Med_12_519
PubMedSearch : Lee_2017_Intern.Emerg.Med_12_519
PubMedID: 27294348

Title : New reference genome sequences of hot pepper reveal the massive evolution of plant disease-resistance genes by retroduplication - Kim_2017_Genome.Biol_18_210
Author(s) : Kim S , Park J , Yeom SI , Kim YM , Seo E , Kim KT , Kim MS , Lee JM , Cheong K , Shin HS , Kim SB , Han K , Lee J , Park M , Lee HA , Lee HY , Lee Y , Oh S , Lee JH , Choi E , Lee SE , Jeon J , Kim H , Choi G , Song H , Lee SC , Kwon JK , Koo N , Hong Y , Kim RW , Kang WH , Huh JH , Kang BC , Yang TJ , Lee YH , Bennetzen JL , Choi D
Ref : Genome Biol , 18 :210 , 2017
Abstract : BACKGROUND: Transposable elements are major evolutionary forces which can cause new genome structure and species diversification. The role of transposable elements in the expansion of nucleotide-binding and leucine-rich-repeat proteins (NLRs), the major disease-resistance gene families, has been unexplored in plants. RESULTS: We report two high-quality de novo genomes (Capsicum baccatum and C. chinense) and an improved reference genome (C. annuum) for peppers. Dynamic genome rearrangements involving translocations among chromosomes 3, 5, and 9 were detected in comparison between C. baccatum and the two other peppers. The amplification of athila LTR-retrotransposons, members of the gypsy superfamily, led to genome expansion in C. baccatum. In-depth genome-wide comparison of genes and repeats unveiled that the copy numbers of NLRs were greatly increased by LTR-retrotransposon-mediated retroduplication. Moreover, retroduplicated NLRs are abundant across the angiosperms and, in most cases, are lineage-specific. CONCLUSIONS: Our study reveals that retroduplication has played key roles for the massive emergence of NLR genes including functional disease-resistance genes in pepper plants.
ESTHER : Kim_2017_Genome.Biol_18_210
PubMedSearch : Kim_2017_Genome.Biol_18_210
PubMedID: 29089032
Gene_locus related to this paper: capch-q75qh4 , capan-a0a1u8fuf5 , capan-a0a1u8gmz3 , capch-a0a2g3bqp0 , capba-a0a2g2vcw4 , capan-a0a1u8flz5 , capch-a0a2g3bau3 , capch-a0a2g3b6c0 , capan-a0a2g2y016 , capch-a0a2g3cje8 , capba-a0a2g2xr67 , capan-a0a1u8fpc9 , capan-a0a1u8fqs3 , capan-a0a1u8ft99 , capan-a0a2g2xtt0 , capan-a0a1u8eu02 , capan-a0a1u8hd13 , capan-a0a2g2y0b6

Title : Chlorpyrifos-induced biomarkers in Japanese medaka (Oryzias latipes) - Jeon_2016_Environ.Sci.Pollut.Res.Int_23_1071
Author(s) : Jeon HJ , Lee YH , Mo HH , Kim MJ , Al-Wabel MI , Kim Y , Cho K , Kim TW , Ok YS , Lee SE
Ref : Environ Sci Pollut Res Int , 23 :1071 , 2016
Abstract : Chlorpyrifos (CHL) is an organophosphate compound that is widely used as an insecticide. Due to its repeated use and high environmental residual property, CHL is frequently passed into aquatic environments by runoff. Consequently, there may be an adverse effect on aquatic vertebrate animals, including fish. Therefore, in this study, we assessed how CHL affected Japanese medaka (Oryzias latipes). The acute toxicity of CHL in adult fish after 96 h of exposure was determined to be 212.50, 266.79, and 412.28 mug L(-1) (LC25, LC50, and LC95, respectively). Acetylcholinesterase (AChE), glutathione S-transferase (GST), and carboxylesterase (CE) activities were obtained from the livers of dead or surviving fish, and the results showed 4.8-fold lower, 4.5-fold higher, and 18.6-fold lower activities for the AChE, GST, and CE, respectively, for 64-h exposure at a concentration of 400 mug L(-1) of CHL. In the embryo toxicity test, curved spines were observed in embryos that were exposed to CHL for 48 h in a concentration-dependent manner. With identification of biomarkers for CHL in the fish, two protein peaks, 5550.86 and 5639.79 m/z, were found to be upregulated. These two proteins can be used as protein biomarkers for CHL contamination in aquatic systems. A phosphatidyl choline with an m/z ratio of 556.32 dramatically decreased after CHL exposure in the fish; thus, it may be considered as a lipid biomarker for CHL. It is assumed as the first report to identify a phospholipid biomarker using a lipidomics approach in fish toxicology. Taken together, these results demonstrated the adverse effects of CHL on Japanese medaka and reveal several candidate biomarkers that can be used as diagnostic tools for determining CHL.
ESTHER : Jeon_2016_Environ.Sci.Pollut.Res.Int_23_1071
PubMedSearch : Jeon_2016_Environ.Sci.Pollut.Res.Int_23_1071
PubMedID: 25966881

Title : Integrated biomarkers induced by chlorpyrifos in two different life stages of zebrafish (Danio rerio) for environmental risk assessment - Jeon_2016_Environ.Toxicol.Pharmacol_43_166
Author(s) : Jeon HJ , Lee YH , Kim MJ , Choi SD , Park BJ , Lee SE
Ref : Environ Toxicol Pharmacol , 43 :166 , 2016
Abstract : This study was performed to understand how chlorpyrifos (CHL) affects zebrafish (Danio rerio) embryos and adults, by exposing this model organism to various concentrations of the insecticide. The 96-h acute toxicity test to determine the effect of CHL on adult zebrafish yielded a LC50 of 709.43mug/L-1. Small molecular weight proteins less than 25kDa and phospholipids were analyzed with MALDI-TOF MS/MS in order to compare expression patterns, revealing that some peaks were dramatically altered after CHL treatment. Whereas no acute toxicity was detected in the embryo toxicity test, malformation of zebrafish larvae was observed, with many individuals harboring curved spines. In an angiogenesis test on larvae of transgenic zebrafish, CHL did not have an inhibitory effect. Relative gene expression analyses using real-time polymerase chain reaction (RT-PCR) of DNA from zebrafish embryos revealed that different subtypes of cytochrome P450 (CYP450), such as CYP1A and CYP3A, were significantly up-regulated in response to CHL at a concentration of 400mug/L-1 compared to the control. The expression level of NR1I2, a CYP gene transcriptional regulator, UGT1a1, and MDR1 were all up-regulated in the CHL-treated embryos. Finally, the expression level of acetylcholinesterase (AChE) and catalase (CAT) decreased, whereas that of superoxide dismutase (SOD) did not differ significantly. Our results suggest that the up-regulation of metabolic enzymes including CYP450 and MDR1 may be involved in CHL resistance in zebrafish.
ESTHER : Jeon_2016_Environ.Toxicol.Pharmacol_43_166
PubMedSearch : Jeon_2016_Environ.Toxicol.Pharmacol_43_166
PubMedID: 26998704

Title : Characterisation and Expression Analysis of MrLip1, a Class 3 Family Lipase of Malassezia restricta - Park_2015_Mycoses_58_671
Author(s) : Park M , Jung WH , Han SH , Lee YH , Lee YW
Ref : Mycoses , 58 :671 , 2015
Abstract : The genus Malassezia is associated with a wide range of skin diseases and is the predominant fungal genus isolated from human skin. Of the 14 Malassezia species identified, M. restricta is the most abundant fungal species found from both healthy and diseased skin. Emerging evidences have suggested that extracellular lipases of Malassezia play a critical role in its survival on the host skin surface. This study aimed to characterise the lipase 1 homologue (MrLip1) in M. restricta and to analyse its expression under different environmental conditions. The full sequence of the gene encoding MrLip1 was determined by rapid amplification of cDNA ends, and it was then heterologously expressed in Pichia pastoris. MrLip1 protein was successfully purified and used for lipase assay and specific antibody generation for use in expression analysis. The optimum pH and temperature for the activity of purified MrLip1 were pH 5.0 and 34 degrees C respectively. Furthermore, the expression of MrLip1 peaked at a similar pH and temperature, suggesting that the optimal conditions for MrLip1 protein activity and expression are similar to that found on the human skin surface. This study provides data to improve our understanding of the role and characteristics of lipase 1 in M. restricta.
ESTHER : Park_2015_Mycoses_58_671
PubMedSearch : Park_2015_Mycoses_58_671
PubMedID: 26404462
Gene_locus related to this paper: 9basi-a0a0k0veq6

Title : Terrein reduces age-related inflammation induced by oxidative stress through Nrf2\/ERK1\/2\/HO-1 signalling in aged HDF cells - Lee_2015_Cell.Biochem.Funct_33_479
Author(s) : Lee YH , Lee SJ , Jung JE , Kim JS , Lee NH , Yi HK
Ref : Cell Biochemistry & Function , 33 :479 , 2015
Abstract : This study investigated whether multiple bioactivity of terrein such as anti-inflammatory and anti-oxidant inhibits age-related inflammation by promoting an antioxidant response in aged human diploid fibroblast (HDF) cells. HDF cells were cultured serially for in vitro replicative senescence. To create the ageing cell phenotype, intermediate stage (PD31) HDF cells were brought to stress-induced premature senescence (SIPS) using hydrogen peroxide (H2 O2). Terrein increased cell viability even with H2O2 stress and reduced inflammatory molecules such as intracellular adhesion molecule-1 (ICAM-1), cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1beta) and tumour necrosis factor-alpha (TNF-alpha). Terrein reduced also phospho-extracellular kinase receptor1/2 (p-EKR1/2) signalling in aged HDF cells. SIPS cells were attenuated for age-related biological markers including reactive oxygen species (ROS), senescence associated beta-galactosidase (SA beta-gal.) and the aforementioned inflammatory molecules. Terrein induced the induction of anti-oxidant molecules, copper/zinc-superoxide defence (Cu/ZnSOD), manganese superoxide dismutase (MnSOD) and heme oxygenase-1 (HO-1) in SIPS cells. Terrein also alleviated reactive oxygen species formation through the Nrf2/HO-1/p-ERK1/2 pathway in aged cells. The results indicate that terrein has an alleviative function of age-related inflammation characterized as an anti-oxidant. Terrein might be a useful nutraceutical compound for anti-ageing.
ESTHER : Lee_2015_Cell.Biochem.Funct_33_479
PubMedSearch : Lee_2015_Cell.Biochem.Funct_33_479
PubMedID: 26416516
Gene_locus related to this paper: aspte-AT1

Title : Synthesis of aminoalkyl-substituted aurone derivatives as acetylcholinesterase inhibitors - Lee_2015_Bioorg.Med.Chem_23_231
Author(s) : Lee YH , Shin MC , Yun YD , Shin SY , Kim JM , Seo JM , Kim NJ , Ryu JH , Lee YS
Ref : Bioorganic & Medicinal Chemistry , 23 :231 , 2015
Abstract : Alzheimer's disease (AD), a progressive and neurodegenerative disorder of the brain, is the most common cause of dementia among elderly people. To date, the successful therapeutic strategy to treat AD is maintaining the levels of acetylcholine by inhibiting acetylcholinesterase (AChE). In the present study, aurone derivatives were designed and synthesized as AChE inhibitors based on the lead structure of sulfuretin. Of those synthesized, compound 10d showed ca. 1700-fold and 6-fold higher AChE inhibitory activity than sulfuretin and galantamine, respectively. This compound also ameliorated scopolamine-induced memory deficit in mice when administered orally at the dose of 1 and 2mg/kg.
ESTHER : Lee_2015_Bioorg.Med.Chem_23_231
PubMedSearch : Lee_2015_Bioorg.Med.Chem_23_231
PubMedID: 25468034

Title : Synthesis of aminoalkyl-substituted coumarin derivatives as acetylcholinesterase inhibitors - Nam_2014_Bioorg.Med.Chem_22_1262
Author(s) : Nam SO , Park DH , Lee YH , Ryu JH , Lee YS
Ref : Bioorganic & Medicinal Chemistry , 22 :1262 , 2014
Abstract : Alzheimer's disease, one of the most common forms of dementia, is a progressive neurodegenerative disorder symptomatically characterized by declines in memory and cognitive abilities. To date, the successful therapeutic strategy to treat AD is maintaining levels of acetylcholine by inhibiting acetylcholinesterase (AChE). In the present study, coumarin derivatives were designed and synthesized as AChE inhibitors based on the lead structure of scopoletin. Of those synthesized, pyrrolidine-substituted coumarins 3b and 3f showed ca. 160-fold higher AChE inhibitory activities than scopoletin. These compounds also ameliorated scopolamine-induced memory deficit in mice when administered orally at the dose of 1 and 2 mg/kg.
ESTHER : Nam_2014_Bioorg.Med.Chem_22_1262
PubMedSearch : Nam_2014_Bioorg.Med.Chem_22_1262
PubMedID: 24485122

Title : Genome sequence of mungbean and insights into evolution within Vigna species - Kang_2014_Nat.Commun_5_5443
Author(s) : Kang YJ , Kim SK , Kim MY , Lestari P , Kim KH , Ha BK , Jun TH , Hwang WJ , Lee T , Lee J , Shim S , Yoon MY , Jang YE , Han KS , Taeprayoon P , Yoon N , Somta P , Tanya P , Kim KS , Gwag JG , Moon JK , Lee YH , Park BS , Bombarely A , Doyle JJ , Jackson SA , Schafleitner R , Srinives P , Varshney RK , Lee SH
Ref : Nat Commun , 5 :5443 , 2014
Abstract : Mungbean (Vigna radiata) is a fast-growing, warm-season legume crop that is primarily cultivated in developing countries of Asia. Here we construct a draft genome sequence of mungbean to facilitate genome research into the subgenus Ceratotropis, which includes several important dietary legumes in Asia, and to enable a better understanding of the evolution of leguminous species. Based on the de novo assembly of additional wild mungbean species, the divergence of what was eventually domesticated and the sampled wild mungbean species appears to have predated domestication. Moreover, the de novo assembly of a tetraploid Vigna species (V. reflexo-pilosa var. glabra) provides genomic evidence of a recent allopolyploid event. The species tree is constructed using de novo RNA-seq assemblies of 22 accessions of 18 Vigna species and protein sets of Glycine max. The present assembly of V. radiata var. radiata will facilitate genome research and accelerate molecular breeding of the subgenus Ceratotropis.
ESTHER : Kang_2014_Nat.Commun_5_5443
PubMedSearch : Kang_2014_Nat.Commun_5_5443
PubMedID: 25384727
Gene_locus related to this paper: vigrr-a0a1s3ue92 , vigrr-a0a1s3v914 , vigrr-a0a1s3tyf7 , phaan-a0a0s3s998 , vigrr-a0a1s3ux10 , vigrr-a0a1s3ubl0 , vigrr-a0a1s3u036 , vigrr-a0a1s3tul4 , phaan-a0a0l9ujf5 , vigrr-a0a1s3tqd3 , vigrr-a0a1s3udb1 , vigrr-a0a1s3ukc0 , vigrr-a0a1s3ur22 , vigrr-a0a3q0ez52 , vigrr-a0a1s3twy0

Title : Draft Genome Sequence of Cladonia macilenta KoLRI003786, a Lichen-Forming Fungus Producing Biruloquinone - Park_2013_Genome.Announc_1_e00695
Author(s) : Park SY , Choi J , Kim JA , Jeong MH , Kim S , Lee YH , Hur JS
Ref : Genome Announc , 1 : , 2013
Abstract : The lichen-forming fungus Cladonia macilenta strain KoLRI003786 is capable of producing an acetylcholinesterase inhibitor, biruloquinone, which effectively prevents neurodegeneration in Alzheimer's disease. Laying the foundation to unravel the biruloquinone biosynthetic pathway, we present the 37.11-Mb draft genome sequence of strain KoLRI003786.
ESTHER : Park_2013_Genome.Announc_1_e00695
PubMedSearch : Park_2013_Genome.Announc_1_e00695
PubMedID: 24009123

Title : The difference in C-reactive protein value between initial and 24 hours follow-up (D-CRP) data as a predictor of mortality in organophosphate poisoned patients - Lee_2013_Clin.Toxicol.(Phila)_51_29
Author(s) : Lee JH , Lee YH , Park YH , Kim YH , Hong CK , Cho KW , Hwang SY
Ref : Clinical Toxicology (Phila) , 51 :29 , 2013
Abstract : Background. Organophosphate poisoning is a worldwide concern and there have been many reports about factors involved in the severity and prognosis of toxicity. The aim of this study was to evaluate the relationship between the serum C-reactive protein activity and clinical outcome in acute organophosphate-poisoned patients. Methods. This was a retrospective cohort study conducted from January 2007 to February 2012. Using a multivariate logistic analysis, data on the total population was retrospectively analyzed for association with mortality. The difference in C-reactive protein value between initial and follow-up after 24 hours (D-CRP) was compared in survivors and non-survivors. The D-CRP, APACHE (Acute Physiology and Chronic Health Evaluation) II scoring system and SOFA (Sequential Organ Failure Assessment) score were compared by analyzing receiver operating characteristic (ROC) curves. Results. Among the 96 subjects, 74 survived and 22 died. In the total population, age, BUN, creatinine, APACHE II and SOFA score, MAP, GCS, hematocrit, respiratory rate, albumin, cholinesterase, and the difference in C-reactive protein value between initial and follow-up after 24 hours (D-CRP) were found to be associated with mortality. The fatality rate of organophosphate poisoning was 22.9% and the D-CRP was found to be associated with a significantly higher risk of death in a multiple logistic regression (Odds ratio = 1.178, 95% CI = 1.049-1.322, p = 0.006). Conclusion. The initial serum C-reactive protein and acetylcholinesterase were not found to be associated with the severity of acute organophosphate poisoning. However, the difference in C-reactive protein value between initial and follow-up after 24 hours (D-CRP) was associated with mortality in the total population of patients with acute organophosphate poisoning.
ESTHER : Lee_2013_Clin.Toxicol.(Phila)_51_29
PubMedSearch : Lee_2013_Clin.Toxicol.(Phila)_51_29
PubMedID: 23170819

Title : Inhaled essential oil from Chamaecyparis obtuse ameliorates the impairments of cognitive function induced by injection of beta-amyloid in rats - Bae_2012_Pharm.Biol_50_900
Author(s) : Bae D , Seol H , Yoon HG , Na JR , Oh K , Choi CY , Lee DW , Jun W , Youl Lee K , Lee J , Hwang K , Lee YH , Kim S
Ref : Pharm Biol , 50 :900 , 2012
Abstract : CONTEXT: Chamaecyparis obtusa Sieb. & Zucc., Endlicher (Cupressaceae) forest bathing or aromatherapy has been shown in various studies to have biological functions such as anticancer, antiallergies, antiinflammatory, and antioxidant activity. However, no reports exist on the pharmacological or biological activities of the essential oil of C. obtusa (EOCO) or its effects on central nervous system. OBJECTIVE: The aggregation and formation of beta-amyloid peptides (Abeta) into fibrils are central events in the pathogenesis of Alzheimer's disease (AD), and overproduction and aggregation of Abeta into oligomers have been known to trigger neurotoxicity. In this study, we investigated the effects of inhaled EOCO on cognitive function and neuronal apoptosis in rats intrahippocampally injected with Abeta. MATERIALS AND METHODS: To model AD, 4 mug of aggregated Abeta was injected into the hippocampus. To test the effects of EOCO, behavioral performance in the Morris water maze was tested 4 days after injection. After behavioral testing, brain sections were prepared for TTC staining and TUNEL assay. RESULTS: Inhaled EOCO protected spatial learning and memory from the impairments induced by Abeta(1-40) injection. In addition, the behavioral deficits accompanying Abeta(1-40)-induced AD were attenuated by inhalation of EOCO. Furthermore, acetylcholinesterase (AChE) activity and neuronal apoptosis were significantly inhibited in rats treated with Abeta(1-40) and EOCO compared to rats treated only with Abeta(1-40). DISCUSSION AND CONCLUSION: EOCO suppressed both AD-related neuronal cell apoptosis and AD-related dysfunction of the memory system. Thus, the results of this study support EOCO as a candidate drug for the treatment of AD.
ESTHER : Bae_2012_Pharm.Biol_50_900
PubMedSearch : Bae_2012_Pharm.Biol_50_900
PubMedID: 22468783

Title : Flavanones inhibit the clonogenicity of HCT116 cololectal cancer cells - Woo_2012_Int.J.Mol.Med_29_403
Author(s) : Woo Y , Shin SY , Hyun J , Lee SD , Lee YH , Lim Y
Ref : Int J Mol Med , 29 :403 , 2012
Abstract : Naringenin has been shown to display various biological effects such as antioxidant, anticancer, anti-inflammatory, and antiviral activities. Taxifolin inhibits the production of lipopolysaccharide-induced prostaglandin E, and fustin suppresses the activity of acetylcholinesterase. They all belong to flavanone which is a class of flavonoids with a C6-C3-C6 skeleton. Since the anticancer activities of flavanone derivatives have rarely been reported, we examined the effects of 26 flavanone derivatives on HCT116 colorectal cancer cells. Our results suggest that flavanone derivatives control the expression of cell cycle regulatory proteins, which blocks G1 cell cycle progression and inhibits the clonogenicity of HCT116 cells. In addition, in order to design flavanone derivatives that show better anticancer activity, structure-activity relationships were examined.
ESTHER : Woo_2012_Int.J.Mol.Med_29_403
PubMedSearch : Woo_2012_Int.J.Mol.Med_29_403
PubMedID: 22160193

Title : Insight into trade-off between wood decay and parasitism from the genome of a fungal forest pathogen - Olson_2012_New.Phytol_194_1001
Author(s) : Olson A , Aerts A , Asiegbu F , Belbahri L , Bouzid O , Broberg A , Canback B , Coutinho PM , Cullen D , Dalman K , Deflorio G , van Diepen LT , Dunand C , Duplessis S , Durling M , Gonthier P , Grimwood J , Fossdal CG , Hansson D , Henrissat B , Hietala A , Himmelstrand K , Hoffmeister D , Hogberg N , James TY , Karlsson M , Kohler A , Kues U , Lee YH , Lin YC , Lind M , Lindquist E , Lombard V , Lucas S , Lunden K , Morin E , Murat C , Park J , Raffaello T , Rouze P , Salamov A , Schmutz J , Solheim H , Stahlberg J , Velez H , de Vries RP , Wiebenga A , Woodward S , Yakovlev I , Garbelotto M , Martin F , Grigoriev IV , Stenlid J
Ref : New Phytol , 194 :1001 , 2012
Abstract : Parasitism and saprotrophic wood decay are two fungal strategies fundamental for succession and nutrient cycling in forest ecosystems. An opportunity to assess the trade-off between these strategies is provided by the forest pathogen and wood decayer Heterobasidion annosum sensu lato. We report the annotated genome sequence and transcript profiling, as well as the quantitative trait loci mapping, of one member of the species complex: H. irregulare. Quantitative trait loci critical for pathogenicity, and rich in transposable elements, orphan and secreted genes, were identified. A wide range of cellulose-degrading enzymes are expressed during wood decay. By contrast, pathogenic interaction between H. irregulare and pine engages fewer carbohydrate-active enzymes, but involves an increase in pectinolytic enzymes, transcription modules for oxidative stress and secondary metabolite production. Our results show a trade-off in terms of constrained carbohydrate decomposition and membrane transport capacity during interaction with living hosts. Our findings establish that saprotrophic wood decay and necrotrophic parasitism involve two distinct, yet overlapping, processes.
ESTHER : Olson_2012_New.Phytol_194_1001
PubMedSearch : Olson_2012_New.Phytol_194_1001
PubMedID: 22463738
Gene_locus related to this paper: 9homo-w4jrb9 , 9homo-w4jsg4 , 9homo-w4kds7 , 9homo-w4jwl9 , 9homo-w4kjy2 , 9homo-w4jw43 , 9homo-w4ka20 , 9homo-w4k8t3 , 9homo-w4jz43 , 9homo-w4k8q2 , 9homo-w4k910 , 9homo-w4k6f5 , 9homo-w4k6j3 , 9homo-w4k8n2 , 9homo-w4jrf3 , 9homo-w4ke07 , 9homo-w4k3i8 , 9homo-w4jqh1 , 9agam-w4k203 , 9agam-w4jpy3 , 9agam-w4jn81 , 9agam-w4jmz2

Title : Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses - O'Connell_2012_Nat.Genet_44_1060
Author(s) : O'Connell RJ , Thon MR , Hacquard S , Amyotte SG , Kleemann J , Torres MF , Damm U , Buiate EA , Epstein L , Alkan N , Altmuller J , Alvarado-Balderrama L , Bauser CA , Becker C , Birren BW , Chen Z , Choi J , Crouch JA , Duvick JP , Farman MA , Gan P , Heiman D , Henrissat B , Howard RJ , Kabbage M , Koch C , Kracher B , Kubo Y , Law AD , Lebrun MH , Lee YH , Miyara I , Moore N , Neumann U , Nordstrom K , Panaccione DG , Panstruga R , Place M , Proctor RH , Prusky D , Rech G , Reinhardt R , Rollins JA , Rounsley S , Schardl CL , Schwartz DC , Shenoy N , Shirasu K , Sikhakolli UR , Stuber K , Sukno SA , Sweigard JA , Takano Y , Takahara H , Trail F , van der Does HC , Voll LM , Will I , Young S , Zeng Q , Zhang J , Zhou S , Dickman MB , Schulze-Lefert P , Ver Loren van Themaat E , Ma LJ , Vaillancourt LJ
Ref : Nat Genet , 44 :1060 , 2012
Abstract : Colletotrichum species are fungal pathogens that devastate crop plants worldwide. Host infection involves the differentiation of specialized cell types that are associated with penetration, growth inside living host cells (biotrophy) and tissue destruction (necrotrophy). We report here genome and transcriptome analyses of Colletotrichum higginsianum infecting Arabidopsis thaliana and Colletotrichum graminicola infecting maize. Comparative genomics showed that both fungi have large sets of pathogenicity-related genes, but families of genes encoding secreted effectors, pectin-degrading enzymes, secondary metabolism enzymes, transporters and peptidases are expanded in C. higginsianum. Genome-wide expression profiling revealed that these genes are transcribed in successive waves that are linked to pathogenic transitions: effectors and secondary metabolism enzymes are induced before penetration and during biotrophy, whereas most hydrolases and transporters are upregulated later, at the switch to necrotrophy. Our findings show that preinvasion perception of plant-derived signals substantially reprograms fungal gene expression and indicate previously unknown functions for particular fungal cell types.
ESTHER : O'Connell_2012_Nat.Genet_44_1060
PubMedSearch : O'Connell_2012_Nat.Genet_44_1060
PubMedID: 22885923
Gene_locus related to this paper: colgm-kex1 , colhi-h1vve5 , colhi-h1vkk5 , colgm-e3qtg4 , colhi-h1vbq5 , colgm-e3qyh7 , colgm-e3q7u5 , colhi-h1vs61 , colgm-e3qip4 , colgm-e3qv97 , colhi-h1v665 , colgm-e3qky4 , colhi-h1vd91 , colhi-h1uvl1 , colhi-h1v7k5 , colgm-e3qu96 , colhi-h1vhh1 , colhi-h1v638 , colhi-h1vcz3 , colgm-e3qwt6 , colgm-e3q3z6 , colhi-h1vmh6 , colgm-e3qqq8 , colhi-h1v0e8 , colgm-e3qyt9 , colgm-e3qby6 , colgm-e3qsm5 , colgm-e3q6f6 , colgm-e3qwt4 , colgm-e3qb89 , colhi-h1vh96 , colgm-e3qwz9 , colgm-e3qbd3 , colgm-e3qtz0 , colhi-h1w5n4 , colgm-e3q7y3 , colgm-e3qpz1 , colhi-h1v2e4 , colgm-e3qux5 , colgm-e3qx16 , colgm-cbpya , colgm-e3q8b3

Title : Comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens - Klosterman_2011_PLoS.Pathog_7_e1002137
Author(s) : Klosterman SJ , Subbarao KV , Kang S , Veronese P , Gold SE , Thomma BP , Chen Z , Henrissat B , Lee YH , Park J , Garcia-Pedrajas MD , Barbara DJ , Anchieta A , de Jonge R , Santhanam P , Maruthachalam K , Atallah Z , Amyotte SG , Paz Z , Inderbitzin P , Hayes RJ , Heiman DI , Young S , Zeng Q , Engels R , Galagan J , Cuomo CA , Dobinson KF , Ma LJ
Ref : PLoS Pathog , 7 :e1002137 , 2011
Abstract : The vascular wilt fungi Verticillium dahliae and V. albo-atrum infect over 200 plant species, causing billions of dollars in annual crop losses. The characteristic wilt symptoms are a result of colonization and proliferation of the pathogens in the xylem vessels, which undergo fluctuations in osmolarity. To gain insights into the mechanisms that confer the organisms' pathogenicity and enable them to proliferate in the unique ecological niche of the plant vascular system, we sequenced the genomes of V. dahliae and V. albo-atrum and compared them to each other, and to the genome of Fusarium oxysporum, another fungal wilt pathogen. Our analyses identified a set of proteins that are shared among all three wilt pathogens, and present in few other fungal species. One of these is a homolog of a bacterial glucosyltransferase that synthesizes virulence-related osmoregulated periplasmic glucans in bacteria. Pathogenicity tests of the corresponding V. dahliae glucosyltransferase gene deletion mutants indicate that the gene is required for full virulence in the Australian tobacco species Nicotiana benthamiana. Compared to other fungi, the two sequenced Verticillium genomes encode more pectin-degrading enzymes and other carbohydrate-active enzymes, suggesting an extraordinary capacity to degrade plant pectin barricades. The high level of synteny between the two Verticillium assemblies highlighted four flexible genomic islands in V. dahliae that are enriched for transposable elements, and contain duplicated genes and genes that are important in signaling/transcriptional regulation and iron/lipid metabolism. Coupled with an enhanced capacity to degrade plant materials, these genomic islands may contribute to the expanded genetic diversity and virulence of V. dahliae, the primary causal agent of Verticillium wilts. Significantly, our study reveals insights into the genetic mechanisms of niche adaptation of fungal wilt pathogens, advances our understanding of the evolution and development of their pathogenesis, and sheds light on potential avenues for the development of novel disease management strategies to combat destructive wilt diseases.
ESTHER : Klosterman_2011_PLoS.Pathog_7_e1002137
PubMedSearch : Klosterman_2011_PLoS.Pathog_7_e1002137
PubMedID: 21829347
Gene_locus related to this paper: vera1-c9srn2 , vera1-c9sn46 , verdv-g2wq49 , verdv-g2xca2 , vera1-c9sea3 , verdv-g2wzn4 , vera1-c9sek2 , verdv-g2wym5 , verdv-g2x5f3 , vera1-c9sx20 , vera1-c9sw01 , verdv-g2x1l1 , verdv-g2wuv4 , verdv-g2xaw5 , verdv-g2wsb2 , verdv-g2wty6 , vera1-c9si59 , verdv-g2xdu9 , vera1-c9s818 , verdv-g2xdr1 , verdv-g2wsw7 , verdv-g2wvq8 , 9pezi-a0a0g4li73 , vera1-kex1 , verdv-g2x8m5

Title : The plant cell wall-decomposing machinery underlies the functional diversity of forest fungi - Eastwood_2011_Science_333_762
Author(s) : Eastwood DC , Floudas D , Binder M , Majcherczyk A , Schneider P , Aerts A , Asiegbu FO , Baker SE , Barry K , Bendiksby M , Blumentritt M , Coutinho PM , Cullen D , de Vries RP , Gathman A , Goodell B , Henrissat B , Ihrmark K , Kauserud H , Kohler A , LaButti K , Lapidus A , Lavin JL , Lee YH , Lindquist E , Lilly W , Lucas S , Morin E , Murat C , Oguiza JA , Park J , Pisabarro AG , Riley R , Rosling A , Salamov A , Schmidt O , Schmutz J , Skrede I , Stenlid J , Wiebenga A , Xie X , Kues U , Hibbett DS , Hoffmeister D , Hogberg N , Martin F , Grigoriev IV , Watkinson SC
Ref : Science , 333 :762 , 2011
Abstract : Brown rot decay removes cellulose and hemicellulose from wood--residual lignin contributing up to 30% of forest soil carbon--and is derived from an ancestral white rot saprotrophy in which both lignin and cellulose are decomposed. Comparative and functional genomics of the "dry rot" fungus Serpula lacrymans, derived from forest ancestors, demonstrated that the evolution of both ectomycorrhizal biotrophy and brown rot saprotrophy were accompanied by reductions and losses in specific protein families, suggesting adaptation to an intercellular interaction with plant tissue. Transcriptome and proteome analysis also identified differences in wood decomposition in S. lacrymans relative to the brown rot Postia placenta. Furthermore, fungal nutritional mode diversification suggests that the boreal forest biome originated via genetic coevolution of above- and below-ground biota.
ESTHER : Eastwood_2011_Science_333_762
PubMedSearch : Eastwood_2011_Science_333_762
PubMedID: 21764756
Gene_locus related to this paper: serl3-f8prj2 , serl3-f8qcc4 , serl9-f8ngp6 , serl9-f8nhd7 , serl9-f8nhq9 , serl9-f8nq77 , serl9-f8nr67 , serl9-f8nrt5 , serl9-f8nvy7.1 , serl9-f8nvy7.2 , serl9-f8nvy8 , serl9-f8nxt0.1 , serl9-f8nxt0.2 , serl9-f8nzr3 , serl9-f8p0f0 , serl9-f8p6v0 , serl9-f8p015 , serl9-f8p018 , serl9-f8p386 , serl9-f8paz8 , serl9-f8pbv1 , serl9-f8pby1 , serl9-f8pc25 , serl9-f8pc39 , serl9-f8nia7 , serl3-f8pju2 , serl9-f8peh1 , serl9-nps3

Title : Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium - Ma_2010_Nature_464_367
Author(s) : Ma LJ , van der Does HC , Borkovich KA , Coleman JJ , Daboussi MJ , Di Pietro A , Dufresne M , Freitag M , Grabherr M , Henrissat B , Houterman PM , Kang S , Shim WB , Woloshuk C , Xie X , Xu JR , Antoniw J , Baker SE , Bluhm BH , Breakspear A , Brown DW , Butchko RA , Chapman S , Coulson R , Coutinho PM , Danchin EG , Diener A , Gale LR , Gardiner DM , Goff S , Hammond-Kosack KE , Hilburn K , Hua-Van A , Jonkers W , Kazan K , Kodira CD , Koehrsen M , Kumar L , Lee YH , Li L , Manners JM , Miranda-Saavedra D , Mukherjee M , Park G , Park J , Park SY , Proctor RH , Regev A , Ruiz-Roldan MC , Sain D , Sakthikumar S , Sykes S , Schwartz DC , Turgeon BG , Wapinski I , Yoder O , Young S , Zeng Q , Zhou S , Galagan J , Cuomo CA , Kistler HC , Rep M
Ref : Nature , 464 :367 , 2010
Abstract : Fusarium species are among the most important phytopathogenic and toxigenic fungi. To understand the molecular underpinnings of pathogenicity in the genus Fusarium, we compared the genomes of three phenotypically diverse species: Fusarium graminearum, Fusarium verticillioides and Fusarium oxysporum f. sp. lycopersici. Our analysis revealed lineage-specific (LS) genomic regions in F. oxysporum that include four entire chromosomes and account for more than one-quarter of the genome. LS regions are rich in transposons and genes with distinct evolutionary profiles but related to pathogenicity, indicative of horizontal acquisition. Experimentally, we demonstrate the transfer of two LS chromosomes between strains of F. oxysporum, converting a non-pathogenic strain into a pathogen. Transfer of LS chromosomes between otherwise genetically isolated strains explains the polyphyletic origin of host specificity and the emergence of new pathogenic lineages in F. oxysporum. These findings put the evolution of fungal pathogenicity into a new perspective.
ESTHER : Ma_2010_Nature_464_367
PubMedSearch : Ma_2010_Nature_464_367
PubMedID: 20237561
Gene_locus related to this paper: fusox-a0a1d3s5h0 , gibf5-fus2 , fusof-f9f2k2 , fusof-f9f3l6 , fusof-f9f6t8 , fusof-f9f6v2 , fusof-f9f132 , fusof-f9f781 , fusof-f9fd72 , fusof-f9fd90 , fusof-f9fem0 , fusof-f9fhk2 , fusof-f9fj19 , fusof-f9fj20 , fusof-f9fki8 , fusof-f9fmx2 , fusof-f9fnt4 , fusof-f9fpy4 , fusof-f9fvs6 , fusof-f9fwu0 , fusof-f9fxz4 , fusof-f9fzy5 , fusof-f9g2a2 , fusof-f9g3b1 , fusof-f9g5h7 , fusof-f9g6e6 , fusof-f9g6y7 , fusof-f9g7b0 , fusof-f9g797 , fusof-f9g972 , fusof-f9ga50 , fusof-f9gck4 , fusof-f9gd15 , gibze-a8w610 , gibze-b1pdn0 , gibze-i1r9e6 , gibze-i1rda9 , gibze-i1rdk7 , gibze-i1rec8 , gibze-i1rgs0 , gibze-i1rgy0 , gibze-i1rh52 , gibze-i1rhi8 , gibze-i1rig9 , gibze-i1rip5 , gibze-i1rpg6 , gibze-i1rsg2 , gibze-i1rv36 , gibze-i1rxm5 , gibze-i1rxp8 , gibze-i1rxv5 , gibze-i1s1u3 , gibze-i1s3j9 , gibze-i1s6l7 , gibze-i1s8i8 , gibze-i1s9x4 , gibze-q4huy1 , gibze-i1rg17 , fuso4-j9mvr9 , fuso4-j9ngs6 , fuso4-j9niq8 , fuso4-j9nqm2 , gibze-i1rb76 , gibze-i1s1m7 , gibze-i1s3z6 , gibze-i1rd78 , gibze-i1rgl9 , gibze-i1rjp7 , gibze-i1s1q6 , gibze-i1ri35 , gibze-i1rf76 , gibze-i1rhp3 , fusc1-n4uj11 , fusc4-n1s9p6 , gibf5-s0dqr2 , gibm7-w7n1b5 , fusof-f9g6q0 , gibm7-w7n497 , fusox-x0bme4 , gibm7-w7mcf8 , gibm7-w7mak5 , fusox-x0a2c5 , gibm7-w7mum7 , fusox-w9iyc7 , gibm7-w7maw6 , gibm7-w7msi0 , gibm7-w7luf0 , gibm7-w7msa3 , gibm7-w7mna8 , gibm7-w7n8b7 , gibm7-w7n564 , fusox-w9jpi0 , gibm7-w7ngc3 , gibm7-w7m4v6 , gibm7-w7m4v2 , gibm7-w7lt61 , gibm7-w7mly6 , gibm7-w7ncn3 , fusox-w9ibd7 , fusof-f9fnm6 , gibm7-w7n526 , gibza-a0a016pda4 , gibza-a0a016pl96 , gibm7-w7muq1 , fusof-f9gfd3 , gibm7-w7mt52 , gibze-i1rjb5 , gibf5-s0ehu3 , fusox-w9hvf0 , gibze-i1rkc4 , gibm7-w7mv30 , gibze-a0a1c3ylb1 , fuso4-a0a0c4diy4 , gibm7-w7n4n0 , gibze-gra11 , gibze-fsl2 , gibf5-fub4 , gibf5-fub5 , gibf5-fus5 , gibm7-dlh1

Title : Enhancement of osteoblast biocompatibility on titanium surface with Terrein treatment - Lee_2010_Cell.Biochem.Funct_28_678
Author(s) : Lee YH , Lee NH , Bhattarai G , Oh YT , Yu MK , Yoo ID , Jhee EC , Yi HK
Ref : Cell Biochemistry & Function , 28 :678 , 2010
Abstract : Titanium is biocompatible with bodily tissues. However, the formation of ROS on the titanium surfaces might have negative response of the activity of the surroundings cells. Terrein was isolated from Penicullium sp. 20135 and found to reduce the effects of LPS-induced inflammation. This study examined the role of Terrein on the biocompatibility of titanium to determine if it can help improve osseointegration. MC-3T3 E1 cells were grown on titanium surfaces. The biocompatibility of Terrein was examined by adding it directly to the culture media at the indicated concentration. The cells on the titanium surface produced excessive ROS and decreased the activity of Cu/Zn SOD and Mn SOD. Moreover, the cells had higher activity towards oxidative stress molecules, such as MAPK, FAK and iNOS expression. In addition, MC-3T3 E1 osteoblast-like cells promoted osteoclast differentiation but reduced osteoblast differentiation and mineralization on the titanium surface. Interestingly, the cells given the Terrein treatment showed higher resistance towards oxidative stress through the up-regulation of ERK1/2 and FAK activity but the down-regulation of SAPK/JNK and iNOS activity. Moreover, Terrein promoted osteoblast differentiation and bone mineralization to elevate the activity of ALP, SPARC and down-regulate RANKL expression after blocking NF-kappaB translocation from the cytosol to the nucleus. In conclusion, the presence of Terrein on titanium surfaces increases osteoblast cell growth without inflammation. Moreover, Terrein, as a putative antioxidant agent, may enhance osseointegration by decreasing the level of ROS and having a potentially synergistic effect on osteoblast differentiation.
ESTHER : Lee_2010_Cell.Biochem.Funct_28_678
PubMedSearch : Lee_2010_Cell.Biochem.Funct_28_678
PubMedID: 21104936
Gene_locus related to this paper: aspte-AT1

Title : The potential effects of ethyl acetate fraction from Curcuma longa L. on lipolysis in differentiated 3T3-L1 adipocytes - Lee_2010_J.Med.Food_13_364
Author(s) : Lee J , Yoon HG , Lee YH , Park J , You Y , Kim K , Jang JY , Yang JW , Jun W
Ref : J Med Food , 13 :364 , 2010
Abstract : The effects of the turmeric ethyl acetate fraction (TEF) from the methanolic extract from Curcuma longa L. on lipid metabolism and underlying mechanisms of lipolysis were investigated in 3T3-L1 adipocytes. The intracellular lipid droplets were stained with Oil red O dye and quantified. Compared to the control, lipid accumulation was significantly decreased by 46.6% with treatment by TEF at the concentration of 20 microg/mL. The intracellular triglyceride (TG) level was also reduced by 37.9% at the concentration of 20 microg/mL. To determine the mechanism for TG content reduction, levels of glucose uptake and glycerol release were measured. Incubation of the 3T3-L1 adipocytes with TEF for 4 hours significantly lowered the cellular level of glucose in a dose-dependent manner. Furthermore, cellular expression of insulin-responsive glucose transporter (GLUT)-4 was decreased by 46%, indicating that reduced glucose uptake was due to a decrease in cellular GLUT-4 expression. In addition, the level of free glycerol released into the cultured medium was increased by 36.4% with the treatment by TEF. In subsequent measurements using quantitative real-time polymerase chain reaction, mRNA levels of hormone-sensitive lipase (HSL) and adipose TG lipase (ATGL) were elevated by 34.8% and 16.9%, respectively, at the concentration of 20 microg/mL. These results suggest that TEF partially inhibits lipogenesis by the suppression of glucose uptake via the decreased expression of cellular GLUT-4 and stimulates lipolysis through the induction of HSL and/or ATGL gene expression, resulting in the increased glycerol release.
ESTHER : Lee_2010_J.Med.Food_13_364
PubMedSearch : Lee_2010_J.Med.Food_13_364
PubMedID: 20412020

Title : X-ray crystallographic analysis of the 6-aminohexanoate cyclic dimer hydrolase: catalytic mechanism and evolution of an enzyme responsible for nylon-6 byproduct degradation - Yasuhira_2010_J.Biol.Chem_285_1239
Author(s) : Yasuhira K , Shibata N , Mongami G , Uedo Y , Atsumi Y , Kawashima Y , Hibino A , Tanaka Y , Lee YH , Kato D , Takeo M , Higuchi Y , Negoro S
Ref : Journal of Biological Chemistry , 285 :1239 , 2010
Abstract : We performed x-ray crystallographic analyses of the 6-aminohexanoate cyclic dimer (Acd) hydrolase (NylA) from Arthrobacter sp., an enzyme responsible for the degradation of the nylon-6 industry byproduct. The fold adopted by the 472-amino acid polypeptide generated a compact mixed alpha/beta fold, typically found in the amidase signature superfamily; this fold was especially similar to the fold of glutamyl-tRNA(Gln) amidotransferase subunit A (z score, 49.4) and malonamidase E2 (z score, 44.8). Irrespective of the high degree of structural similarity to the typical amidase signature superfamily enzymes, the specific activity of NylA for glutamine, malonamide, and indoleacetamide was found to be lower than 0.5% of that for Acd. However, NylA possessed carboxylesterase activity nearly equivalent to the Acd hydrolytic activity. Structural analysis of the inactive complex between the activity-deficient S174A mutant of NylA and Acd, performed at 1.8 A resolution, suggested the following enzyme/substrate interactions: a Ser(174)-cis-Ser(150)-Lys(72) triad constitutes the catalytic center; the backbone N in Ala(171) and Ala(172) are involved in oxyanion stabilization; Cys(316)-S(gamma) forms a hydrogen bond with nitrogen (Acd-N(7)) at the uncleaved amide bond in two equivalent amide bonds of Acd. A single S174A, S150A, or K72A substitution in NylA by site-directed mutagenesis decreased the Acd hydrolytic and esterolytic activities to undetectable levels, indicating that Ser(174)-cis-Ser(150)-Lys(72) is essential for catalysis. In contrast, substitutions at position 316 specifically affected Acd hydrolytic activity, suggesting that Cys(316) is responsible for Acd binding. On the basis of the structure and functional analysis, we discussed the catalytic mechanisms and evolution of NylA in comparison with other Ser-reactive hydrolases.
ESTHER : Yasuhira_2010_J.Biol.Chem_285_1239
PubMedSearch : Yasuhira_2010_J.Biol.Chem_285_1239
PubMedID: 19889645

Title : Expression of esterase gene in yeast for organophosphates biodegradation - Kambiranda_2009_Pestic.Biochem.Physiol_94_15
Author(s) : Kambiranda DM , Asraful-Islam SMd , Cho KM , Math RK , Lee YH , Kim H , Yun HD
Ref : Pesticide Biochemistry and Physiology , 94 :15 , 2009
Abstract : Organophosphates are esters of phosphoric acid and can be hydrolyzed and detoxified by carboxylesterase and phosphotriesterase. In this work esterase enzyme (Est5S) was expressed in yeast to demonstrate the organophosphorus hydrolytic activity from a metagenomic library of cow rumen bacteria. The esterase gene (est5S) is 1098 bp in length, encoding a protein of 366 amino acid residues with a molecular weight of 40 kDa. Est5S enzyme was successfully produced by Pichia pastoris at a high expression level of approximately 4.0 g L-1. With p-nitrophenol butyrate as the substrate, the optimal temperature and pH for enzyme activity were determined to be 40 C and pH 7.0, respectively. The esterase enzyme was tested for degradation of chlorpyrifos (CP). TLC results obtained inferred that CP could be degraded by esterase enzyme (Est5S) and HPLC results revealed that CP could be efficiently degraded up to 100 ppm. Cadusafos (CS), coumaphos (CM), diazinon (DZ) dyfonate (DF), ethoprophos (EP), fenamiphos (FM), methylparathion (MPT), and parathion (PT) were also degraded up to 68, 60, 80, 40, 45, 60, 95, and 100%, respectively, when used as a substrate with Est5S protein. The results highlight the potential use of this enzyme in the cleanup of contaminated insecticides.
ESTHER : Kambiranda_2009_Pestic.Biochem.Physiol_94_15
PubMedSearch : Kambiranda_2009_Pestic.Biochem.Physiol_94_15
Gene_locus related to this paper: 9bact-q0gpb3

Title : Cooperation and functional diversification of two closely related galactolipase genes for jasmonate biosynthesis - Hyun_2008_Dev.Cell_14_183
Author(s) : Hyun Y , Choi S , Hwang HJ , Yu J , Nam SJ , Ko J , Park JY , Seo YS , Kim EY , Ryu SB , Kim WT , Lee YH , Kang H , Lee I
Ref : Dev Cell , 14 :183 , 2008
Abstract : Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response. Chloroplast lipid hydrolysis is a critical step for JA biosynthesis, but the mechanism of this process remains elusive. We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A(1) activity. DGL is expressed in the leaves and has a specific role in maintaining basal JA content under normal conditions, and this expression regulates vegetative growth and is required for a rapid JA burst after wounding. During wounding, DGL and DAD1 have partially redundant functions for JA production, but they show different induction kinetics, indicating temporally separated roles: DGL plays a role in the early phase of JA production, and DAD1 plays a role in the late phase of JA production. Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression.
ESTHER : Hyun_2008_Dev.Cell_14_183
PubMedSearch : Hyun_2008_Dev.Cell_14_183
PubMedID: 18267087
Gene_locus related to this paper: arath-AT4G16820 , arath-PLA11 , arath-PLA12 , arath-PLA13 , arath-PLA15 , arath-PLA16 , arath-PLA17

Title : Terrein reduces pulpal inflammation in human dental pulp cells - Lee_2008_J.Endod_34_433
Author(s) : Lee JC , Yu MK , Lee R , Lee YH , Jeon JG , Lee MH , Jhee EC , Yoo ID , Yi HK
Ref : J Endod , 34 :433 , 2008
Abstract : Terrein is a bioactive fungal metabolite whose anti-inflammatory properties are virtually unknown. The purpose of this study was to determine the effects of terrein on lipopolysaccharide (LPS)-induced expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in human dental pulp cells and to determine the mechanism of the observed effects. The LPS-induced expression of ICAM-1 and VCAM-1 was inhibited by terrein in both a time- and dose-dependent manner. LPS-stimulated translocation of nuclear factor kappa B (NF-kappaB) into the nucleus, which was blocked by inhibitors of amino kinase terminal (AKT, LY294002), extracellular signal regulated kinase 1/2 (ERK 1/2, PD98059), p38 (SB203580), and c-jun NH2-terminal kinase (JNK, SP600125) or terrein. In addition, these inhibitors and terrein also reduced the level of ICAM-1 and VCAM-1 expression in LPS-induced inflammation of pulp cells. Terrein suppressed NF-kappaB activation by blocking the activation of Akt. These results strongly suggest the potential role of terrein as an anti-inflammatory modulator in pulpal inflammation.
ESTHER : Lee_2008_J.Endod_34_433
PubMedSearch : Lee_2008_J.Endod_34_433
PubMedID: 18358890
Gene_locus related to this paper: aspte-AT1

Title : The cannabinoid CB1 receptor is expressed in pancreatic delta-cells - Tharp_2008_Biochem.Biophys.Res.Commun_372_595
Author(s) : Tharp WG , Lee YH , Maple RL , Pratley RE
Ref : Biochemical & Biophysical Research Communications , 372 :595 , 2008
Abstract : Antagonists of cannabinoid CB1 receptor (CB1, CNR1) promote weight loss and decrease hyperglycemia in patients with type 2 diabetes. While the endocannabinoid system may modulate islet hormone secretion, the cell-type expressing CB1 receptor in islets has not been fully resolved. In this study, we verified receptor gene expression in rodent islets and cell lines and examined the distribution of CB1 receptor in mouse, rat, and human islets by confocal immunofluorescence (IF) microscopy. IF demonstrated CB1 receptor was present in beta-cell lines, but co-localized solely with somatostatin in the islet delta-cells of Zucker rats, C57BL/6 mice, and humans; no CB1 receptor expression was observed in alpha-, beta-, or pp-cells. Similarly, a rat somatostatinoma cell line, MSL-G2-Tu6, was found to express CB1 receptor. We also found monoacylglycerol lipase (MAGL) to be expressed in delta-cells and fatty acid amide hydrolase (FAAH) to be expressed in alpha-cells. The specific expression of CB1 in delta-cells suggests that the ECS may play a role in modulating islet hormone secretion. As there are some differences between our findings and previous reports, further studies, including detailed physiological studies of the effects of the ECS on islet function, are warranted.
ESTHER : Tharp_2008_Biochem.Biophys.Res.Commun_372_595
PubMedSearch : Tharp_2008_Biochem.Biophys.Res.Commun_372_595
PubMedID: 18505678

Title : Molecular cloning and functional expression of esf gene encoding enantioselective lipase from Serratia marcescens ES-2 for kinetic resolution of optically active (S)-flurbiprofen - Lee_2007_J.Microbiol.Biotechnol_17_74
Author(s) : Lee KW , Bae HA , Lee YH
Ref : J Microbiol Biotechnol , 17 :74 , 2007
Abstract : An enantioselective lipase gene (esf) for the kinetic resolution of optically active (S)-flurbiprofen was cloned from the new strain Serratia marcescens ES-2. The esf gene was composed of a 1,845-bp open reading frame encoding 614 amino acid residues with a calculated molecular mass of 64,978 Da. The lipase expressed in E. coli was purified by a three-step procedure, and it showed preferential substrate specificity toward the medium-chain-length fatty acids. The esf gene encoding the enantioselective lipase was reintroduced into the parent strain S. marcescens ES-2 for secretory overexpression. The transformant S. marcescens BESF secreted up to 217 kU/ ml of the enantioselective lipase, about 54-fold more than the parent strain, after supplementing 3.0% Triton X-207. The kinetic resolution of (S)-flurbiprofen was carried out even at an extremely high (R,S)-flurbiprofen ethyl ester [(R,S)-FEE] concentration of 500 mM, 130 kU of the S. marcescens ES-2 lipase per mmol of (R,S)-FEE, and 1,000 mM of succinyl beta-cyclodextrin as the dispenser at 37 degrees C for 12 h, achieving the high enantiomeric excess and conversion yield of 98% and 48%, respectively.
ESTHER : Lee_2007_J.Microbiol.Biotechnol_17_74
PubMedSearch : Lee_2007_J.Microbiol.Biotechnol_17_74
PubMedID: 18051356
Gene_locus related to this paper: serma-lipasA

Title : Proteome analysis of Paenibacillus polymyxa E681 affected by barley - Seul_2007_J.Microbiol.Biotechnol_17_934
Author(s) : Seul KJ , Park SH , Ryu CM , Lee YH , Ghim SY
Ref : J Microbiol Biotechnol , 17 :934 , 2007
Abstract : Paenibacillus polymyxa E681 is known to be able to suppress plant diseases by producing antimicrobial compounds and to promote plant growth by producing phytohormones, and secreting diverse degrading enzymes. In spite of these capabilities, little is known regarding the flow of information from the bacterial strain to the barley roots. In an attempt to determine the flow of information from the bacterial strain to barley roots, the train was grown in the presence and absence of barley, and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and MALDI-TOF mass spectrometry were used. 2D-PAGE detected approximately 1000 spots in the cell and 1100 spots in the supernatant at a pH 4-10 gradient. Interestingly, about 80 spots from each sample showed quantitative variations. Fifty-three spots from these were analyzed by MALDI-TOF mass spectrometry and 28 proteins were identified. Most of the cytosolic proteins expressed at higher levels were found in P. polymyxa E681 cells grown in the presence of barley rather than in the absence of barley. Proteins detected at a lower level in the surpernatant of P. polymyxa E681 cells grown in the presence of barley were lipoprotein, glucose-6-phosphate 1-dehydrogenase, heat-shock protein HtpG spermidine synthase, OrfZ, ribonuclease PH, and coenzyme PQQ synthesis protein, and flagellar hook-associated protein 2 whereas proteins detected at a higher level in the surpernatant of P. polymyxa E681 cells grown in the presence of barley included D-alanyl-D-alanine ligase A, isopentenyldiphosphate delta-isomerase, ABC transporter ATP-binding protein Uup, lipase. Many of the proteins belonging to plant-induced stimulons are associated with biosynthetic metabolism and metabolites of proteins and transport. Some of these proteins would be expected to be induced by environmental changes resulting from the accumulation of plant-secreted substances.
ESTHER : Seul_2007_J.Microbiol.Biotechnol_17_934
PubMedSearch : Seul_2007_J.Microbiol.Biotechnol_17_934
PubMedID: 18050911

Title : Molecular analysis of an inactive aflatoxin biosynthesis gene cluster in Aspergillus oryzae RIB strains - Tominaga_2006_Appl.Environ.Microbiol_72_484
Author(s) : Tominaga M , Lee YH , Hayashi R , Suzuki Y , Yamada O , Sakamoto K , Gotoh K , Akita O
Ref : Applied Environmental Microbiology , 72 :484 , 2006
Abstract : To help assess the potential for aflatoxin production by Aspergillus oryzae, the structure of an aflatoxin biosynthesis gene homolog cluster in A. oryzae RIB 40 was analyzed. Although most genes in the corresponding cluster exhibited from 97 to 99% similarity to those of Aspergillus flavus, three genes shared 93% similarity or less. A 257-bp deletion in the aflT region, a frameshift mutation in norA, and a base pair substitution in verA were found in A. oryzae RIB 40. In the aflR promoter, two substitutions were found in one of the three putative AreA binding sites and in the FacB binding site. PCR primers were designed to amplify homologs of aflT, nor-1, aflR, norA, avnA, verB, and vbs and were used to detect these genes in 210 A. oryzae strains. Based on the PCR results, the A. oryzae RIB strains were classified into three groups, although most of them fell into two of the groups. Group 1, in which amplification of all seven genes was confirmed, contained 122 RIB strains (58.1% of examined strains), including RIB 40. Seventy-seven strains (36.7%) belonged to group 2, characterized by having only vbs, verB, and avnA in half of the cluster. Although slight expression of aflR was detected by reverse transcription-PCR in some group 1 strains, including RIB 40, other genes (avnA, vbs, verB, and omtA) related to aflatoxin production were not detected. aflR was not detected in group 2 strains by Southern analysis.
ESTHER : Tominaga_2006_Appl.Environ.Microbiol_72_484
PubMedSearch : Tominaga_2006_Appl.Environ.Microbiol_72_484
PubMedID: 16391082
Gene_locus related to this paper: aspor-PKSL1 , asppa-q6ueg5

Title : The genome sequence of the rice blast fungus Magnaporthe grisea - Dean_2005_Nature_434_980
Author(s) : Dean RA , Talbot NJ , Ebbole DJ , Farman ML , Mitchell TK , Orbach MJ , Thon M , Kulkarni R , Xu JR , Pan H , Read ND , Lee YH , Carbone I , Brown D , Oh YY , Donofrio N , Jeong JS , Soanes DM , Djonovic S , Kolomiets E , Rehmeyer C , Li W , Harding M , Kim S , Lebrun MH , Bohnert H , Coughlan S , Butler J , Calvo S , Ma LJ , Nicol R , Purcell S , Nusbaum C , Galagan JE , Birren BW
Ref : Nature , 434 :980 , 2005
Abstract : Magnaporthe grisea is the most destructive pathogen of rice worldwide and the principal model organism for elucidating the molecular basis of fungal disease of plants. Here, we report the draft sequence of the M. grisea genome. Analysis of the gene set provides an insight into the adaptations required by a fungus to cause disease. The genome encodes a large and diverse set of secreted proteins, including those defined by unusual carbohydrate-binding domains. This fungus also possesses an expanded family of G-protein-coupled receptors, several new virulence-associated genes and large suites of enzymes involved in secondary metabolism. Consistent with a role in fungal pathogenesis, the expression of several of these genes is upregulated during the early stages of infection-related development. The M. grisea genome has been subject to invasion and proliferation of active transposable elements, reflecting the clonal nature of this fungus imposed by widespread rice cultivation.
ESTHER : Dean_2005_Nature_434_980
PubMedSearch : Dean_2005_Nature_434_980
PubMedID: 15846337
Gene_locus related to this paper: maggr-a4qqu1 , maggr-a4uuq1 , mago7-g4n0f1 , maggr-a4qy60 , maggr-a4qyj3 , maggr-a4r8c0 , maggr-a4r257 , maggr-a4rd24 , maggr-a4ri35 , maggr-a4rlz4 , maggr-a4rme6 , maggr-q0pnd2 , maggr-q0pnd5 , maggr-q2keh4 , maggr-q2khf5 , mago7-a4qsp1 , mago7-a4qt55 , mago7-a4qua7 , mago7-a4qup0 , mago7-a4qvx8 , mago7-a4qwz1 , mago7-a4qx26 , mago7-a4qxi6 , mago7-a4qz39 , mago7-a4qzg2 , mago7-a4r4e9 , mago7-a4r4n4 , mago7-a4r6f4 , mago7-a4r106 , mago7-a4ra37 , mago7-a4rdm3 , mago7-a4rgp8 , mago7-a4rlj9 , mago7-a4rpg7 , mago7-a4uc22 , mago7-dapb , mago7-g4mk92 , mago7-g4mkc6 , mago7-g4mkk9 , mago7-g4mns9 , mago7-g4ms19 , mago7-g4mvm8 , mago7-g4mvw5 , mago7-g4mvw6 , mago7-g4n6j4 , mago7-g4nal1 , mago7-g4naw0 , mago7-g4nba0 , mago7-g4nbs0 , mago7-g4nc41 , mago7-g4ncz9 , mago7-g4nhn9 , mago7-g4nil3 , mago7-g4nky6 , mago7-g5ehg6 , mago7-g5ehv6 , mago7-q2kh83 , mago7-q2khe7 , mago7-a4qxp0 , mago7-g4nih2 , mago7-g4mr24 , mago7-g4nff5 , mago7-g4n8c3 , mago7-g4ni03 , mago7-g4nhm4 , mago7-g4nfb6 , mago7-g4mmn3 , mago7-g4mqv7 , mago7-g4mzv6 , mago7-g4nbz1 , mago7-g4ms46 , mago7-g4n0h2 , mago7-g4nev7 , mago7-g4msm5 , magoy-l7i6m7 , mago7-g4ne75 , magor-a0a4p7n714 , magor-a0a4p7nig7 , mago7-g4mzi2 , mago7-cbpya , mago7-kex1 , mago7-g4n703

Title : Isolation and characterization of Acinetobacter sp. ES-1 excreting a lipase with high enantioselectivity for (S)-ketoprofen ethyl ester - Lee_2004_Biotechnol.Lett_26_1639
Author(s) : Lee KW , Shin GS , Bae HA , Shin HD , Lee YH
Ref : Biotechnol Lett , 26 :1639 , 2004
Abstract : A new Acinetobacter sp. ES-1, grown on triolein, tryptone and Triton X-100, excreted a lipase that hydrolyzed 10 mM (R,S)-ketoprofen ethyl ester into (S)-ketoprofen. The crude lipase had an activity of 10 U ml(-1) and, at 30 degrees C and pH 7 over 48 h, gave a conversion yield of 35% with an enantiomeric excess for the product 96%.
ESTHER : Lee_2004_Biotechnol.Lett_26_1639
PubMedSearch : Lee_2004_Biotechnol.Lett_26_1639
PubMedID: 15604812

Title : Targeted disruption of the microsomal epoxide hydrolase gene. Microsomal epoxide hydrolase is required for the carcinogenic activity of 7,12-dimethylbenz[a]anthracene - Miyata_1999_J.Biol.Chem_274_23963
Author(s) : Miyata M , Kudo G , Lee YH , Yang TJ , Gelboin HV , Fernandez-Salguero P , Kimura S , Gonzalez FJ
Ref : Journal of Biological Chemistry , 274 :23963 , 1999
Abstract : Microsomal epoxide hydrolase (mEH) is a conserved enzyme that is known to hydrolyze many drugs and carcinogens, and a few endogenous steroids and bile acids. mEH-null mice were produced and found to be fertile and have no phenotypic abnormalities thus indicating that mEH is not critical for reproduction and physiological homeostasis. mEH has also been implicated in participating in the metabolic activation of polycyclic aromatic hydrocarbon carcinogens. Embryonic fibroblast derived from the mEH-null mice were unable to produce the proximate carcinogenic metabolite of 7,12-dimethylbenz[a]anthracene (DMBA), a widely studied experimental prototype for the polycylic aromatic hydrocarbon class of chemical carcinogens. They were also resistant to DMBA-mediated toxicity. Using the two-stage initiation-promotion skin cancer bioassay, the mEH-null mice were found to be highly resistant to DMBA-induced carcinogenesis. In a complete carcinogenesis bioassay, the mEH mice were totally resistant to tumorigenesis. These data establish in an intact animal model that mEH is a key genetic determinant in DMBA carcinogenesis through its role in production of the ultimate carcinogenic metabolite of DMBA, the 3,4-diol-1,2-epoxide.
ESTHER : Miyata_1999_J.Biol.Chem_274_23963
PubMedSearch : Miyata_1999_J.Biol.Chem_274_23963
PubMedID: 10446164