Domann E

References (4)

Title : Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes - Hain_2012_BMC.Genomics_13_144
Author(s) : Hain T , Ghai R , Billion A , Kuenne CT , Steinweg C , Izar B , Mohamed W , Mraheil MA , Domann E , Schaffrath S , Karst U , Goesmann A , Oehm S , Puhler A , Merkl R , Vorwerk S , Glaser P , Garrido P , Rusniok C , Buchrieser C , Goebel W , Chakraborty T
Ref : BMC Genomics , 13 :144 , 2012
Abstract : BACKGROUND: Listeria monocytogenes is a food-borne pathogen that causes infections with a high-mortality rate and has served as an invaluable model for intracellular parasitism. Here, we report complete genome sequences for two L. monocytogenes strains belonging to serotype 4a (L99) and 4b (CLIP80459), and transcriptomes of representative strains from lineages I, II, and III, thereby permitting in-depth comparison of genome- and transcriptome -based data from three lineages of L. monocytogenes. Lineage III, represented by the 4a L99 genome is known to contain strains less virulent for humans.
RESULTS: The genome analysis of the weakly pathogenic L99 serotype 4a provides extensive evidence of virulence gene decay, including loss of several important surface proteins. The 4b CLIP80459 genome, unlike the previously sequenced 4b F2365 genome harbours an intact inlB invasion gene. These lineage I strains are characterized by the lack of prophage genes, as they share only a single prophage locus with other L. monocytogenes genomes 1/2a EGD-e and 4a L99. Comparative transcriptome analysis during intracellular growth uncovered adaptive expression level differences in lineages I, II and III of Listeria, notable amongst which was a strong intracellular induction of flagellar genes in strain 4a L99 compared to the other lineages. Furthermore, extensive differences between strains are manifest at levels of metabolic flux control and phosphorylated sugar uptake. Intriguingly, prophage gene expression was found to be a hallmark of intracellular gene expression. Deletion mutants in the single shared prophage locus of lineage II strain EGD-e 1/2a, the lma operon, revealed severe attenuation of virulence in a murine infection model. CONCLUSION: Comparative genomics and transcriptome analysis of L. monocytogenes strains from three lineages implicate prophage genes in intracellular adaptation and indicate that gene loss and decay may have led to the emergence of attenuated lineages.
ESTHER : Hain_2012_BMC.Genomics_13_144
PubMedSearch : Hain_2012_BMC.Genomics_13_144
PubMedID: 22530965
Gene_locus related to this paper: lismo-LMO0110 , lismo-LMO0493 , lismo-LMO0580 , lismo-LMO0950 , lismo-LMO0951 , lismo-LMO1128 , lismo-LMO1258 , lismo-LMO2089 , lismo-LMO2433 , lismo-LMO2578 , lismo-LMO2755 , lismo-metx

Title : Complete genome sequence of Listeria seeligeri, a nonpathogenic member of the genus Listeria - Steinweg_2010_J.Bacteriol_192_1473
Author(s) : Steinweg C , Kuenne CT , Billion A , Mraheil MA , Domann E , Ghai R , Barbuddhe SB , Karst U , Goesmann A , Puhler A , Weisshaar B , Wehland J , Lampidis R , Kreft J , Goebel W , Chakraborty T , Hain T
Ref : Journal of Bacteriology , 192 :1473 , 2010
Abstract : We report the complete and annotated genome sequence of the nonpathogenic Listeria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria.
ESTHER : Steinweg_2010_J.Bacteriol_192_1473
PubMedSearch : Steinweg_2010_J.Bacteriol_192_1473
PubMedID: 20061480
Gene_locus related to this paper: lisin-LIN0976 , lismo-LMO2452 , lismo-metx , lisss-d3ulc5 , lisss-d3unw0 , lisss-d3urb3 , lisss-d3urc9

Title : Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes - Hain_2006_J.Bacteriol_188_7405
Author(s) : Hain T , Steinweg C , Kuenne CT , Billion A , Ghai R , Chatterjee SS , Domann E , Karst U , Goesmann A , Bekel T , Bartels D , Kaiser O , Meyer F , Puhler A , Weisshaar B , Wehland J , Liang C , Dandekar T , Lampidis R , Kreft J , Goebel W , Chakraborty T
Ref : Journal of Bacteriology , 188 :7405 , 2006
Abstract : We present the complete genome sequence of Listeria welshimeri, a nonpathogenic member of the genus Listeria. Listeria welshimeri harbors a circular chromosome of 2,814,130 bp with 2,780 open reading frames. Comparative genomic analysis of chromosomal regions between L. welshimeri, Listeria innocua, and Listeria monocytogenes shows strong overall conservation of synteny, with the exception of the translocation of an F(o)F(1) ATP synthase. The smaller size of the L. welshimeri genome is the result of deletions in all of the genes involved in virulence and of "fitness" genes required for intracellular survival, transcription factors, and LPXTG- and LRR-containing proteins as well as 55 genes involved in carbohydrate transport and metabolism. In total, 482 genes are absent from L. welshimeri relative to L. monocytogenes. Of these, 249 deletions are commonly absent in both L. welshimeri and L. innocua, suggesting similar genome evolutionary paths from an ancestor. We also identified 311 genes specific to L. welshimeri that are absent in the other two species, indicating gene expansion in L. welshimeri, including horizontal gene transfer. The species L. welshimeri appears to have been derived from early evolutionary events and an ancestor more compact than L. monocytogenes that led to the emergence of nonpathogenic Listeria spp.
ESTHER : Hain_2006_J.Bacteriol_188_7405
PubMedSearch : Hain_2006_J.Bacteriol_188_7405
PubMedID: 16936040
Gene_locus related to this paper: lisin-LIN0976 , lisin-LIN2544 , lismo-LMO2089 , lismo-LMO2452 , lismo-LMO2453 , lismo-metx , lisss-d3urb3 , lisw6-a0agy6 , lisw6-a0ajc8 , lisw6-a0am12

Title : Comparative genomics of Listeria species - Glaser_2001_Science_294_849
Author(s) : Glaser P , Frangeul L , Buchrieser C , Rusniok C , Amend A , Baquero F , Berche P , Bloecker H , Brandt P , Chakraborty T , Charbit A , Chetouani F , Couve E , de Daruvar A , Dehoux P , Domann E , Dominguez-Bernal G , Duchaud E , Durant L , Dussurget O , Entian KD , Fsihi H , Portillo FG , Garrido P , Gautier L , Goebel W , Gomez-Lopez N , Hain T , Hauf J , Jackson D , Jones LM , Kaerst U , Kreft J , Kuhn M , Kunst F , Kurapkat G , Madueno E , Maitournam A , Vicente JM , Ng E , Nedjari H , Nordsiek G , Novella S , de Pablos B , Perez-Diaz JC , Purcell R , Remmel B , Rose M , Schlueter T , Simoes N , Tierrez A , Vazquez-Boland JA , Voss H , Wehland J , Cossart P
Ref : Science , 294 :849 , 2001
Abstract : Listeria monocytogenes is a food-borne pathogen with a high mortality rate that has also emerged as a paradigm for intracellular parasitism. We present and compare the genome sequences of L. monocytogenes (2,944,528 base pairs) and a nonpathogenic species, L. innocua (3,011,209 base pairs). We found a large number of predicted genes encoding surface and secreted proteins, transporters, and transcriptional regulators, consistent with the ability of both species to adapt to diverse environments. The presence of 270 L. monocytogenes and 149 L. innocua strain-specific genes (clustered in 100 and 63 islets, respectively) suggests that virulence in Listeria results from multiple gene acquisition and deletion events.
ESTHER : Glaser_2001_Science_294_849
PubMedSearch : Glaser_2001_Science_294_849
PubMedID: 11679669
Gene_locus related to this paper: lisin-LIN0589 , lisin-LIN0754 , lisin-LIN0850 , lisin-LIN0949 , lisin-LIN0950 , lisin-LIN0976 , lisin-LIN1094 , lisin-LIN1546 , lisin-LIN1782 , lisin-LIN2180 , lisin-LIN2214 , lisin-LIN2363 , lisin-LIN2527 , lisin-LIN2544 , lisin-LIN2547 , lisin-LIN2722 , lisin-LIN2825 , lisin-LIN2898 , lismc-c1l0d9 , lismo-LMO0110 , lismo-LMO0493 , lismo-LMO0580 , lismo-LMO0752 , lismo-LMO0760 , lismo-LMO0857 , lismo-LMO0950 , lismo-LMO0951 , lismo-LMO0977 , lismo-LMO1128 , lismo-LMO1258 , lismo-LMO1511 , lismo-LMO1674 , lismo-LMO2074 , lismo-LMO2089 , lismo-LMO2109 , lismo-LMO2262 , lismo-LMO2433 , lismo-LMO2450 , lismo-LMO2452 , lismo-LMO2453 , lismo-LMO2578 , lismo-LMO2677 , lismo-LMO2755 , lismo-metx