Hu H

References (34)

Title : Analysis of Genes Associated with Feeding Preference and Detoxification in Various Developmental Stages of Aglais urticae - Xi_2024_Insects_15_
Author(s) : Xi O , Guo W , Hu H
Ref : Insects , 15 : , 2024
Abstract : Herbivorous insects and host plants have developed a close and complex relationship over a long period of co-evolution. Some plants provide nutrients for insects, but plants' secondary metabolites also influence their growth and development. Urtica cannabina roots and leaves are poisonous, yet Aglais urticae larvae feed on them, so we aimed to clarify the mechanism enabling this interaction. At present, studies on the detoxification mechanism of the A. urticae are rare. In our study, first, we used the A. urticae larval odor selection behavior bioassay and choice feeding preference assay to analyze the feeding preferences of A. urticae on its host plant, U. cannabina. Next, we used transcriptome sequencing to obtain the unigenes annotated and classified by various databases, such as KEGG and GO. In this study, we found that U. cannabina could attract A. urticae larvae to feed via scent, and the feeding preference assay confirmed that larvae preferred U. cannabina leaves over three other plants: Cirsium japonicum, Cannabis sativa, and Arctium lappa. The activity of detoxifying enzymes GST and CarE changed in larvae that had consumed U. cannabina. Furthermore, through transcriptomic sequencing analysis, 77,624 unigenes were assembled from raw reads. The numbers of differentially expressed genes were calculated using pairwise comparisons of all life stages; the expression of detoxification enzyme genes was substantially higher in larvae than in the pupal and adult stages. Finally, we identified and summarized 34 genes associated with detoxification enzymes, such as UDP-glucose 4-epimerase gene, 5 Glutathione S-transferase genes, 4 Carboxylesterase genes, 4 Cytochrome P450 genes, 10 ATP-binding cassette genes, 4 Superoxide dismutase, and Peroxidase. Moreover, we identified 28 genes associated with the development of A. urticae. The qRT-PCR results were nearly consistent with the transcriptomic data, showing an increased expression level of four genes in larvae. Taken together, this study examines the correlation between A. urticae and host plants U. cannabina, uncovering a pronounced preference for A. urticae larvae toward host plants. Consistent with RNA-seq, we investigated the mechanism of A. urticae's interaction with host plants and identified detoxification-related genes. The present study provides theoretical support for studying insect adaptation mechanisms and biological control.
ESTHER : Xi_2024_Insects_15_
PubMedSearch : Xi_2024_Insects_15_
PubMedID: 38249036

Title : Monovalent SARS-COV-2 mRNA vaccine using optimal UTRs and LNPs is highly immunogenic and broadly protective against Omicron variants - Ye_2023_Proc.Natl.Acad.Sci.U.S.A_120_e2311752120
Author(s) : Ye Z , Bonam SR , McKay LGA , Plante JA , Walker J , Zhao Y , Huang C , Chen J , Xu C , Li Y , Liu L , Harmon J , Gao S , Song D , Zhang Z , Plante KS , Griffiths A , Hu H , Xu Q
Ref : Proc Natl Acad Sci U S A , 120 :e2311752120 , 2023
Abstract : The emergence of highly transmissible severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) that are resistant to the current COVID-19 vaccines highlights the need for continued development of broadly protective vaccines for the future. Here, we developed two messenger RNA (mRNA)-lipid nanoparticle (LNP) vaccines, TU88mCSA and ALCmCSA, using the ancestral SARS-CoV-2 spike sequence, optimized 5' and 3' untranslated regions (UTRs), and LNP combinations. Our data showed that these nanocomplexes effectively activate CD4(+) and CD8(+) T cell responses and humoral immune response and provide complete protection against WA1/2020, Omicron BA.1 and BQ.1 infection in hamsters. Critically, in Omicron BQ.1 challenge hamster models, TU88mCSA and ALCmCSA not only induced robust control of virus load in the lungs but also enhanced protective efficacy in the upper respiratory airways. Antigen-specific immune analysis in mice revealed that the observed cross-protection is associated with superior UTRs [Carboxylesterase 1d (Ces1d)/adaptor protein-3beta (AP3B1)] and LNP formulations that elicit robust lung tissue-resident memory T cells. Strong protective effects of TU88mCSA or ALCmCSA against both WA1/2020 and VOCs suggest that this mRNA-LNP combination can be a broadly protective vaccine platform in which mRNA cargo uses the ancestral antigen sequence regardless of the antigenic drift. This approach could be rapidly adapted for clinical use and timely deployment of vaccines against emerging and reemerging VOCs.
ESTHER : Ye_2023_Proc.Natl.Acad.Sci.U.S.A_120_e2311752120
PubMedSearch : Ye_2023_Proc.Natl.Acad.Sci.U.S.A_120_e2311752120
PubMedID: 38134199

Title : Tributyl phosphate can inhibit the feeding behavior of rotifers by altering the axoneme structure, neuronal coordination and energy supply required for motile cilia - Zhang_2023_J.Hazard.Mater_459_132224
Author(s) : Zhang X , Tang X , Yang Y , Tong X , Hu H
Ref : J Hazard Mater , 459 :132224 , 2023
Abstract : Organophosphorus flame retardants (OPFRs) are frequently detected in aquatic environments and can potentially amplify the food chain, posing a potential risk to organisms. Marine invertebrates have primitive nervous systems to regulate behavior, but how they respond to OPFRs that are potentially neurotoxic substances is unclear. This study assessed changes in the feeding behavior of rotifer Brachionus plicatilis exposed to alkyl OPFRs tributyl phosphate (TnBP) (0.376 nM, 3.76 and 22.53 microM) to elucidate the mechanism of behavioral toxicity. TnBP at 22.53 microM reduced the ingestion and filtration rates of rotifers for Chlorella vulgaris and Phaeocystis globosa in a 24-h test and altered rotifer-P. globosa population dynamics in 15-d coculture. Ciliary beat frequency was also reduced, and the expression of genes encoding the cilia axoneme was downregulated. TnBP could inhibit rotifer acetylcholinesterase activity by binding this protein and reduce the expression of the exocytotic membrane protein syntaxin-4, suggesting a disorder in nervous regulation of cilia beat. Moreover, TnBP induced abnormal shape and dysfunction of mitochondria, which caused insufficient energy required for ciliary movement. This study revealed diverse neurotoxicity mechanisms of TnBP, particularly as a potentially competing acetylcholinesterase ligand for aquatic invertebrates. Our research also provides a meaningful reference for OPFR-induced behavioral toxicity assessments.
ESTHER : Zhang_2023_J.Hazard.Mater_459_132224
PubMedSearch : Zhang_2023_J.Hazard.Mater_459_132224
PubMedID: 37557041

Title : Design of 2,5-furandicarboxylic based polyesters degraded in different environmental conditions: Comprehensive experimental and theoretical study - Hu_2022_J.Hazard.Mater_425_127752
Author(s) : Hu H , Li J , Luo S , Tian Y , Wang J , Zhao YL , Zhang R , Zhu J
Ref : J Hazard Mater , 425 :127752 , 2022
Abstract : Nowadays, the promotion and application of aliphatic-aromatic copolyesters, such as poly (butylene adipate-co-terephthalate) (PBAT), are growing into a general trend. Although the structures of diacids exerted substantial impacts on degradation behavior, the underlying mechanisms have rarely been studied. In this work, 2,5-Furandicarboxylic acid was combined with succinic acid (PBSF), adipic acid (PBAF) and diglycolic acid (PBDF) to prepare three kinds of copolyesters. They showed unique degradation behaviors in buffer, enzyme environment and artificial seawater. These characteristics are closely related to the structural compositions of diacids. PBAFs displayed impressive biodegradability when catalyzed by Candida antarctica lipase B (CALB), while the more hydrophilic PBDFs exhibited faster hydrolysis in both buffer and artificial seawater. PBSFs, with hydrophobic and short segments, obtained a relatively slower rate of hydrolysis and enzymatic degradation. The reactivity sites and hydrolytic pathway were revealed by the combination of DFT calculation and Fukui function analysis. MD simulations, QM/MM optimizations and theozyme calculations showed that PBAF-CALB was prone to form a pre-reaction state, leading to the reduced energy barrier in the acylation process. This work revealed the effects of different structural features of diacids on polymer degradation and paved a way to design target biodegradable polymers in different degradation conditions.
ESTHER : Hu_2022_J.Hazard.Mater_425_127752
PubMedSearch : Hu_2022_J.Hazard.Mater_425_127752
PubMedID: 34906869
Gene_locus related to this paper: canar-LipB

Title : CGI-58 Protein Acts as a Positive Regulator of Triacylglycerol Accumulation in Phaeodactylum tricornutum - Shu_2022_J.Microbiol.Biotechnol_33_1
Author(s) : Shu Q , Pan Y , Hu H
Ref : J Microbiol Biotechnol , 33 :1 , 2022
Abstract : Comparative gene identification-58 (CGI-58) is an activating protein of triacylglycerol (TAG) lipase. It has a variety of catalytic activities whereby it may play different roles in diverse organisms. In this study, a homolog of CGI-58 in Phaeodactylum tricornutum (PtCGI-58) was identified. PtCGI-58 was localized in mitochondria by GFP fusion protein analysis, which is different from the reported subcellular localization of CGI-58 in animals and plants. Respectively, PtCGI-58 overexpression resulted in increased neutral lipid content and TAG accumulation by 42-46% and 21-32%. Likewise, it also increased the relative content of eicosapentaenoic acid (EPA), and in particular, the EPA content in TAGs almost doubled. Transcript levels of genes involved in de novo fatty acid synthesis and mitochondrial beta-oxidation were significantly upregulated in PtCGI-58 overexpression strains compared with wild-type cells. Our findings suggest that PtCGI-58 may mediate the breakdown of lipids in mitochondria and the recycling of acyl chains derived from mitochondrial beta-oxidation into TAG biosynthesis. Moreover, this study potentially illuminates new functions for CGI-58 in lipid homeostasis and provides a strategy to enrich EPA in algal TAGs.
ESTHER : Shu_2022_J.Microbiol.Biotechnol_33_1
PubMedSearch : Shu_2022_J.Microbiol.Biotechnol_33_1
PubMedID: 36524337

Title : A stable biosensor based on chitosan-modified graphene for detecting organophosphorus pesticides - Zhang_2021_Biotechnol.Appl.Biochem__
Author(s) : Zhang J , Hu H , Wang P , Zhang C , Wuma J , Yang L
Ref : Biotechnol Appl Biochem , : , 2021
Abstract : An acetylcholinesterase (AChE) biosensor was successfully fabricated with a stable structure and high detection accuracy. Graphene (Gra) nano-fragments modified with chitosan and acetylcholinesterase were successively drip-coated on the surface of a glassy carbon electrode via a layer-by-layer assembly method. The concentration range of the sensor to detect dichlorvos was 0.1 nM to 100000 nM, and the limit of detection was 54 pM. Chitosan (CS) was used to modify graphene for the first time, which enhanced the mechanical flexibility of these graphene nanostructures, significantly improving the stability and detection accuracy of this sensor. This article is protected by copyright. All rights reserved.
ESTHER : Zhang_2021_Biotechnol.Appl.Biochem__
PubMedSearch : Zhang_2021_Biotechnol.Appl.Biochem__
PubMedID: 33660328

Title : General features to enhance enzymatic activity of poly(ethylene terephthalate) hydrolysis - Chen_2021_Nat.Catal_4_425
Author(s) : Chen CC , Han X , Li X , Jiang P , Niu D , Ma L , Liu W , Li S , Qu Y , Hu H , Min J , Yang Y , Zhang L , Zeng W , Huang JW , Dai L , Guo RT , Chen, CC
Ref : Nature Catalysis , 4 :425 , 2021
Abstract : Poly(ethylene terephthalate) (PET) is the most abundant polyester plastic and a major contributor to plastic pollution. IsPETase, from the PET-assimilating bacterium Ideonella sakaiensis, is a unique PET-hydrolytic enzyme that shares high sequence identity to canonical cutinases, but shows substrate preference towards PET and exhibits higher PET-hydrolytic activity at ambient temperature. Structural analyses suggest that IsPETase harbours a substrate-binding residue, W185, with a wobbling conformation and a highly flexible W185-locating beta6-beta7 loop. Here, we show that these features result from the presence of S214 and I218 in IsPETase, whose equivalents are strictly His and Phe, respectively, in all other homologous enzymes. We found that mutating His/Phe residues to Ser/Ile could enhance the PET-hydrolytic activity of several IsPETase-like enzymes. In conclusion, the Ser/Ile mutations should provide an important strategy to improve the activity of potential PET-hydrolytic enzymes with properties that may be useful for various applications.
ESTHER : Chen_2021_Nat.Catal_4_425
PubMedSearch : Chen_2021_Nat.Catal_4_425
PubMedID:
Gene_locus related to this paper: 9burk-a0a1f4jxw8 , idesa-peth

Title : Enhancing PET hydrolytic enzyme activity by fusion of the cellulose-binding domain of cellobiohydrolase I from Trichoderma reesei - Dai_2021_J.Biotechnol_334_47
Author(s) : Dai L , Qu Y , Huang JW , Hu Y , Hu H , Li S , Chen CC , Guo RT
Ref : J Biotechnol , 334 :47 , 2021
Abstract : The large amounts of polyethylene terephthalate (PET) that enter and accumulate in the environment have posed a serious threat to global ecosystems and human health. A PET hydrolase from PET-assimilating bacterium Ideonella sakaiensis (IsPETase) that exhibits superior PET hydrolytic activity at mild conditions is attracting enormous attention in development of plastic biodegrading strategies. In order to enhance the PET hydrolysis capacity of IsPETase, we selected several polymer-binding domains that can adhere to a hydrophobic polymer surface and fused these to a previously engineered IsPETase(S121E/D186H/R280A) (IsPETase(EHA)) variant. We found that fusing a cellulose-binding domain (CBM) of cellobiohydrolase I from Trichoderma reesei onto the C-terminus of IsPETase(EHA) showed a stimulatory effect on enzymatic hydrolysis of PET. Compared to the parental enzyme, IsPETase(EHA)_CBM exhibited 71.5 % and 44.5 % higher hydrolytic activity at 30 degC and 40 degC, respectively. The catalytic activity of IsPETase(EHA)_CBM was increased by 86 % when the protein concentration was increased from 2.5 microg/mL to 20microg/mL. These findings suggest that the fusion of polymer-binding module to IsPETase is a promising strategy to stimulate the enzymatic hydrolysis of PET.
ESTHER : Dai_2021_J.Biotechnol_334_47
PubMedSearch : Dai_2021_J.Biotechnol_334_47
PubMedID: 34044062
Gene_locus related to this paper: idesa-peth

Title : Development of enzymatic electrochemical biosensors for organophosphorus pesticide detection - Hu_2020_J.Environ.Sci.Health.B__1
Author(s) : Hu H , Yang L
Ref : J Environ Sci Health B , :1 , 2020
Abstract : The enzymatic electrochemical biosensor has the advantages of simple operation, speed, and integration in the detection of organophosphorus pesticide (OPs) residues. It has the potential to become the best alternative to the traditional OP detection technology. This article introduces the OP identification principle of different enzymes, the OP detection mechanism of several common sensors, and the enzyme assembly method. In addition, the article discusses application of nanomaterials in sensor preparation and sensor performance parameters in the past decade. The related content of early sensors is outside the scope of this article.
ESTHER : Hu_2020_J.Environ.Sci.Health.B__1
PubMedSearch : Hu_2020_J.Environ.Sci.Health.B__1
PubMedID: 33284686

Title : GDSL lipase occluded stomatal pore 1 is required for wax biosynthesis and stomatal cuticular ledge formation - Tang_2020_New.Phytol_228_1880
Author(s) : Tang J , Yang X , Xiao C , Li J , Chen Y , Li R , Li S , Lu S , Hu H
Ref : New Phytol , 228 :1880 , 2020
Abstract : The plant leaf surface is coated with a waterproof cuticle layer. Cuticle facing the stomatal pore surface needs to be sculpted to form outer cuticular ledge (OCL) after stomatal maturation for efficient gas exchange. Here, we characterized the roles of Arabidopsis GDSL lipase, Occlusion of Stomatal Pore 1 (OSP1), in wax biosynthesis and stomatal OCL formation. OSP1 mutation results in significant reduction in leaf wax synthesis and occlusion of stomata, leading to increased epidermal permeability, decreased transpiration rate, and enhanced drought tolerance. We demonstrated that OSP1 activity is critical for its role in wax biosynthesis and stomatal function. In vitro enzymatic assays demonstrated that OSP1 possesses thioesterase activity, particularly on C22:0 and C26:0 acyl-CoAs. Genetic interaction analyses with CER1 (ECERIFERUM 1), CER3 (ECERIFERUM 3) and MAH1 (Mid-chain Alkane Hydroxylase 1) in wax biosynthesis and stomatal OCL formation showed that OSP1 may act upstream of CER3 in wax biosynthesis, and implicate that wax composition percentage changes and keeping ketones in a lower level play roles, at least partially, in forming stomatal ledges. Our findings provided insights into the molecular mechanism mediating wax biosynthesis and highlighted the link between wax biosynthesis and the process of stomatal OCL formation.
ESTHER : Tang_2020_New.Phytol_228_1880
PubMedSearch : Tang_2020_New.Phytol_228_1880
PubMedID: 32542680

Title : Identification of Highly Selective Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) Inhibitors by a Covalent Fragment-Based Approach - Huang_2020_J.Med.Chem_63_7052
Author(s) : Huang F , Hu H , Wang K , Peng C , Xu W , Zhang Y , Gao J , Liu Y , Zhou H , Huang R , Li M , Shen J , Xu Y
Ref : Journal of Medicinal Chemistry , 63 :7052 , 2020
Abstract : Covalent ligands are of great interest as therapeutic drugs or biochemical tools. Here, we reported the discovery of highly selective and irreversible inhibitors of lipoprotein-associated phospholipase A2 (Lp-PLA2) using a covalent fragment-based approach. The crystal structure of Lp-PLA2 in complex with a covalent fragment not only reveals the covalent reaction mechanism but also provides a good starting point to design compound 8, which has a more than 130,000-fold and 3900-fold increase in potency and selectivity, respectively, compared to those of the covalent fragment. Furthermore, fluorescent probes with high selectivity and sensitivity are developed to characterize Lp-PLA2 and its enzymatic activity in vitro or even in living cells in a way more convenient than immunoblotting tests or immunofluorescence imaging. Overall, we provide a paradigm for application of the covalent fragment-based strategy in covalent ligand discovery and the advantage of enol-cyclocarbamate as a new warhead in designing covalent inhibitors of serine hydrolases.
ESTHER : Huang_2020_J.Med.Chem_63_7052
PubMedSearch : Huang_2020_J.Med.Chem_63_7052
PubMedID: 32459096
Gene_locus related to this paper: human-PLA2G7

Title : Acetylcholinesterase electrochemical biosensors with graphene-transition metal carbides nanocomposites modified for detection of organophosphate pesticides - Wang_2020_PLoS.One_15_e0231981
Author(s) : Wang B , Li Y , Hu H , Shu W , Yang L , Zhang J
Ref : PLoS ONE , 15 :e0231981 , 2020
Abstract : An acetylcholinesterase biosensor modified with graphene and transition metal carbides was prepared to detect organophosphorus pesticides. Cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy were used to characterize the electrochemical catalysis of the biosensor: acetylcholinesterase/chitosan-transition metal carbides/graphene/glassy carbon electrode. With the joint modification of graphene and transition metal carbides, the biosensor has a good performance in detecting dichlorvos with a linear relationship from 11.31 muM to 22.6 nM and the limit of detection was 14.45 nM. Under the premise of parameter optimization, the biosensor showed a good catalytic performance for acetylcholine. Compared to the biosensors without modification, it expressed a better catalytic performance due to the excellent electrical properties, biocompatibility and high specific surface area of graphene, transition metal carbides. Finally, the biosensor exhibits good stability, which can be stored at room temperature for one month without significant performance degradation, and has practical potential for sample testing.
ESTHER : Wang_2020_PLoS.One_15_e0231981
PubMedSearch : Wang_2020_PLoS.One_15_e0231981
PubMedID: 32348360

Title : miR-4454 up-regulated by HPV16 E6\/E7 promotes invasion and migration by targeting ABHD2\/NUDT21 in cervical cancer - Wang_2020_Biosci.Rep_40_
Author(s) : Wang H , Hu H , Luo Z , Liu S , Wu W , Zhu M , Wang J , Liu Y , Lu Z
Ref : Bioscience Reports , 40 : , 2020
Abstract : The abnormal expression of HPV16 E6/E7 activates oncogenes and/or inactivates tumor suppressor genes, resulting in the selective growth and malignant transformation of cancer cells. miR-4454 was selected by sequencing due to its abnormal high expression in HPV16 E6/E7 positive CaSki cell compared with HPV16 E6/E7 negative C33A cell. Overexpression of miR-4454 enhances cervical cancer cell invasion and migration. ABHD2 and NUDT21 are identified as a target gene of miR-4454.The effects of ABHD2 and NUDT21 on migration and invasion of CaSki and C33A cells were determined. The dual luciferase and RT-qPCR assays confirmed that miR-4454 might regulate its targets ABHD2 and NUDT21 to promote the proliferation, invasion and migration, whereas, inhibit the apoptosis in CaSki and C33A cells.
ESTHER : Wang_2020_Biosci.Rep_40_
PubMedSearch : Wang_2020_Biosci.Rep_40_
PubMedID: 32816024

Title : Increased cross-linking micelle retention in the brain of Alzheimer's disease mice by elevated asparagine endopeptidase protease responsive aggregation - Ren_2020_Biomater.Sci_8_6533
Author(s) : Ren J , Jiang F , Wang M , Hu H , Zhang B , Chen L , Dai F
Ref : Biomater Sci , 8 :6533 , 2020
Abstract : Current forms of medication for Alzheimer's disease (AD) provide a symptomatic benefit limited to those with early onset, but there is no single drug available for later stage patients. Given the recent failures of AD drugs in clinical trials, an intensive treatment strategy based on drug combination that is approved is attractive. At present, the greatest difficulty lies in the low accumulation of drugs in the brain. All hydrophilic drugs are limited by the physical and biochemical barriers within the blood-brain barrier and lipophilic drugs are often transported back into the blood by efflux pumps located in the blood-brain barrier. Here, we select elevated asparagine endopeptidase (AEP) as a target to trigger in situ cross-linking of small sized particles to form large sized drug clusters to block the efflux of the brain. Subsequently, responsive cross-linking micelles (RCMs) loaded with the acetylcholinesterase inhibitor, donepezil (DON), the microtubule therapeutic agent, Paclitaxel (PTX), and the glucose metabolism disorder regulator, insulin (INS) are investigated, with a focus on high levels of drug accumulation in the brain in AD. These smart multi-drug delivery RCMs provide a powerful system for AD treatment and can be adapted for other central nervous system (CNS) disorders.
ESTHER : Ren_2020_Biomater.Sci_8_6533
PubMedSearch : Ren_2020_Biomater.Sci_8_6533
PubMedID: 33111725

Title : Hemimetabolous genomes reveal molecular basis of termite eusociality - Harrison_2018_Nat.Ecol.Evol_2_557
Author(s) : Harrison MC , Jongepier E , Robertson HM , Arning N , Bitard-Feildel T , Chao H , Childers CP , Dinh H , Doddapaneni H , Dugan S , Gowin J , Greiner C , Han Y , Hu H , Hughes DST , Huylmans AK , Kemena C , Kremer LPM , Lee SL , Lopez-Ezquerra A , Mallet L , Monroy-Kuhn JM , Moser A , Murali SC , Muzny DM , Otani S , Piulachs MD , Poelchau M , Qu J , Schaub F , Wada-Katsumata A , Worley KC , Xie Q , Ylla G , Poulsen M , Gibbs RA , Schal C , Richards S , Belles X , Korb J , Bornberg-Bauer E
Ref : Nat Ecol Evol , 2 :557 , 2018
Abstract : Around 150 million years ago, eusocial termites evolved from within the cockroaches, 50 million years before eusocial Hymenoptera, such as bees and ants, appeared. Here, we report the 2-Gb genome of the German cockroach, Blattella germanica, and the 1.3-Gb genome of the drywood termite Cryptotermes secundus. We show evolutionary signatures of termite eusociality by comparing the genomes and transcriptomes of three termites and the cockroach against the background of 16 other eusocial and non-eusocial insects. Dramatic adaptive changes in genes underlying the production and perception of pheromones confirm the importance of chemical communication in the termites. These are accompanied by major changes in gene regulation and the molecular evolution of caste determination. Many of these results parallel molecular mechanisms of eusocial evolution in Hymenoptera. However, the specific solutions are remarkably different, thus revealing a striking case of convergence in one of the major evolutionary transitions in biological complexity.
ESTHER : Harrison_2018_Nat.Ecol.Evol_2_557
PubMedSearch : Harrison_2018_Nat.Ecol.Evol_2_557
PubMedID: 29403074
Gene_locus related to this paper: blage-a0a2p8y5s3 , blage-a0a2p8yjf8.2 , blage-a0a2p8xjb6

Title : Expression, functional analysis and mutation of a novel neutral zearalenone-degrading enzyme - Wang_2018_Int.J.Biol.Macromol_118_1284
Author(s) : Wang M , Yin L , Hu H , Selvaraj JN , Zhou Y , Zhang G
Ref : Int J Biol Macromol , 118 :1284 , 2018
Abstract : The crops and grains were often contaminated by high level of mycotoxin zearalenone (ZEN). In order to remove ZEN and keep food safe, ZEN-degrading or detoxifying enzymes are urgently needed. Here, a newly identified lactonohydrolase responsible for the detoxification of ZEN, annotated as Zhd518, was expressed and characterized. Zhd518 showed 65% amino acid identity with Zhd101, which was widely studied for its ZEN-degrading ability. A detailed activity measurement method of ZEN-degrading enzyme was provided. Biochemical analysis indicated that the purified recombinant Zhd518 from E. coli exhibited a high activity against ZEN (207.0 U/mg), with the optimal temperature and pH of 40 degreeC and 8.0, respectively. The Zhd518 can degrade ZEN derivatives, and the specific activities against alpha-Zearalenol, beta-Zearalenol, alpha-Zearalanol and beta-Zearalanol were 23.0 U/mg, 64.7 U/mg, 119.8 U/mg and 66.5 U/mg, respectively. The active sites of Zhd518 were predicted by structure modeling and determined by mutation analysis. A point mutant N156H exhibited 3.3-fold activity against alpha-Zearalenol comparing to Zhd518. Zhd518 is the first reported neutral and the second characterized ZEN-degrading enzyme, which provides a new and more excellent candidate for ZEN detoxifying in food and feed industry.
ESTHER : Wang_2018_Int.J.Biol.Macromol_118_1284
PubMedSearch : Wang_2018_Int.J.Biol.Macromol_118_1284
PubMedID: 29949749
Gene_locus related to this paper: 9euro-a0a0d2ilk1

Title : Sensitive and Selective Detection of Oxo-Form Organophosphorus Pesticides Based on CdSe\/ZnS Quantum Dots - Wei_2017_Molecules_22_
Author(s) : Wei J , Cao J , Hu H , Yang Q , Yang F , Wan J , Su H , He C , Li P , Wang Y
Ref : Molecules , 22 : , 2017
Abstract : A rapid, sensitive and enzyme-based optical biosensor was applied for the determination of seven organophosphorus pesticides (OPPs), including the oxo forms (malaoxon, paraoxon, dibrom, and dichlorvos), the thio forms (malathion and parathion) and the mixed form (demeton) in Panax ginseng. The principal of the proposed method is that the fluorescence quenching effect of quantum dots (QDs) can be observed by enzyme-generated H(2)O(2). The active centers of acetylcholinesterase (AChE) could be inhibited in the presence of pesticides, which caused decrease of the generated H(2)O(2). Then, the inhibition efficiency of pesticide to AChE activity could be evaluated by measuring the fluorescence changes. Different from biosensors based on immobilized enzyme or self-assembling technique, the proposed biosensor demonstrated a good selectivity for the detection of oxo forms of OPPs. In the present study, the important experimental conditions of the proposed biosensor were investigated. Under the optimized conditions (incubation temperature, 35 degrees C; incubation time, 20 min; pH value, 8.0; detection time, 30 min; AChE concentration, 40.9 U/L; and choline oxidase (ChOx) concentration, 637.5 U/L), the limit of detection for the investigated oxo-form OPPs was no more than 0.05 muM, which suggested that the proposed method could be used for sensitive and selective determination of trace amounts of OPPs residues in real samples with complex matrices.
ESTHER : Wei_2017_Molecules_22_
PubMedSearch : Wei_2017_Molecules_22_
PubMedID: 28846648

Title : Resurfaced fluorescent protein as a sensing platform for label-free detection of copper(II) ion and acetylcholinesterase activity - Lei_2015_Anal.Chem_87_1974
Author(s) : Lei C , Wang Z , Nie Z , Deng H , Hu H , Huang Y , Yao S
Ref : Analytical Chemistry , 87 :1974 , 2015
Abstract : Protein engineering by resurfacing is an efficient approach to provide new molecular toolkits for biotechnology and bioanalytical chemistry. H39GFP is a new variant of green fluorescent protein (GFP) containing 39 histidine residues in the primary sequence that was developed by protein resurfacing. Herein, taking H39GFP as the signal reporter, a label-free fluorometric sensor for Cu(2+) sensing was developed based on the unique multivalent metal ion-binding property of H39GFP and fluorescence quenching effect of Cu(2+) by electron transfer. The high affinity of H39GFP with Cu(2+) (Kd, 16.2 nM) leads to rapid detection of Cu(2+) in 5 min with a low detection limit (50 nM). Using acetylthiocholine (ATCh) as the substrate, this H39GFP/Cu(2+) complex-based sensor was further applied for the turn-on fluorescence detection of acetylcholinesterase (AChE) activity. The assay was based on the reaction between Cu(2+) and thiocholine, the hydrolysis product of ATCh by AChE. The proposed sensor is highly sensitive (limit of detection (LOD) = 0.015 mU mL(-1)) and is feasible for screening inhibitors of AChE. Furthermore, the practicability of this method was demonstrated by the detection of pesticide residue (carbaryl) in real food samples. Hence, the successful applications of H39GFP in the detection of metal ion and enzyme activity present the prospect of resurfaced proteins as versatile biosensing platforms.
ESTHER : Lei_2015_Anal.Chem_87_1974
PubMedSearch : Lei_2015_Anal.Chem_87_1974
PubMedID: 25560517

Title : [Cloning of feruloyl esterase gene from Aspergillus niger h408 and high-efficient expression in Pichia pastoris] - Zhou_2014_Wei.Sheng.Wu.Xue.Bao_54_876
Author(s) : Zhou Y , Liu X , Chen J , Hu H , Hou Y
Ref : Wei Sheng Wu Xue Bao , 54 :876 , 2014
Abstract : OBJECTIVE: To achieve the high-efficiency expression of feruloyl estrase gene (AnfaeA) from Aspergillus niger h408 in Pichia pastoris and characterize the recombinant feruloyl esterase (FAE).
METHODS: Using gene splicing by overlap extension (SOE), we cloned AnfaeA gene from A. niger h408 and subcloned into T vector for sequencing analysis. The expression vector pPIC9K-Anfae was constructed by the ligation of the Anfae A gene into the shuttle vector pPIC9K. The plasmid pPIC9K-Anfae was linearized and then electrotransformed into P. pastoris GS115. The recombinant strain with high level of FAE activity was obtained through plate screening. Effects of pH and temperature on recombinant FAE were determined by ultraviolet (UV) methods.
RESULTS: We have successfully cloned and high-efficiently expressed the AnfaeA gene (GenBank: KF911349) from A. niger h408 in P. pastoris GS115. The sequencing result showed that the length of Anfae A was 783bp. The gene contained an Open Reading Frame encoding 260 amino acids and was similar to feruloyl esterase A from A. niger by homology analysis. The deduced amino acids contained a typical active lid and catalytic triad of lipase. The SDS-PAGE result indicated that molecular weight of the recombinant FAE was about 30 kDa and the activity of the recombinant enzyme was 24.72 U/mL. The specific activity of the recombinant FAE was 40.84 U/mg. Compared with A. niger h408, the recombinant enzyme activity increased about to 1100 times. The optimal temperature and pH for recombinant FAE was 50 degrees C and 5.0, respectively. Recombinant FAE showed nearly 80% of its maximal activity at 60 degrees C and was active in the pH range 4.0-9.0.
CONCLUSIONS: The high-efficient expression of AnfaeA gene in P. pastoris provided a prerequisite for achieving industrial application in feed and paper-making industry. In addition, the results established the experimental basis for further improvement of recombinant feruloyl esterase by directed evolution.
ESTHER : Zhou_2014_Wei.Sheng.Wu.Xue.Bao_54_876
PubMedSearch : Zhou_2014_Wei.Sheng.Wu.Xue.Bao_54_876
PubMedID: 25345018

Title : Molecular traces of alternative social organization in a termite genome - Terrapon_2014_Nat.Commun_5_3636
Author(s) : Terrapon N , Li C , Robertson HM , Ji L , Meng X , Booth W , Chen Z , Childers CP , Glastad KM , Gokhale K , Gowin J , Gronenberg W , Hermansen RA , Hu H , Hunt BG , Huylmans AK , Khalil SM , Mitchell RD , Munoz-Torres MC , Mustard JA , Pan H , Reese JT , Scharf ME , Sun F , Vogel H , Xiao J , Yang W , Yang Z , Zhou J , Zhu J , Brent CS , Elsik CG , Goodisman MA , Liberles DA , Roe RM , Vargo EL , Vilcinskas A , Wang J , Bornberg-Bauer E , Korb J , Zhang G , Liebig J
Ref : Nat Commun , 5 :3636 , 2014
Abstract : Although eusociality evolved independently within several orders of insects, research into the molecular underpinnings of the transition towards social complexity has been confined primarily to Hymenoptera (for example, ants and bees). Here we sequence the genome and stage-specific transcriptomes of the dampwood termite Zootermopsis nevadensis (Blattodea) and compare them with similar data for eusocial Hymenoptera, to better identify commonalities and differences in achieving this significant transition. We show an expansion of genes related to male fertility, with upregulated gene expression in male reproductive individuals reflecting the profound differences in mating biology relative to the Hymenoptera. For several chemoreceptor families, we show divergent numbers of genes, which may correspond to the more claustral lifestyle of these termites. We also show similarities in the number and expression of genes related to caste determination mechanisms. Finally, patterns of DNA methylation and alternative splicing support a hypothesized epigenetic regulation of caste differentiation.
ESTHER : Terrapon_2014_Nat.Commun_5_3636
PubMedSearch : Terrapon_2014_Nat.Commun_5_3636
PubMedID: 24845553
Gene_locus related to this paper: zoone-a0a067r283 , zoone-a0a067qst6 , zoone-a0a067rbc7 , zoone-a0a067qz43 , zoone-a0a067qn94 , zoone-a0a067rbw9 , zoone-a0a067qx93 , zoone-a0a067rcf4 , zoone-a0a067r8q8 , zoone-a0a067rh81 , zoone-a0a067r506 , zoone-a0a067qxd4 , zoone-a0a067qy86 , zoone-a0a067qsw2 , zoone-a0a067qfp9 , zoone-a0a067ru91 , zoone-a0a067rwu7 , zoone-a0a067rmu8 , zoone-a0a067r773 , zoone-a0a067qlt8 , zoone-a0a067qhm6 , zoone-a0a067qjz2 , zoone-a0a067qs20 , zoone-a0a067rmu4 , zoone-a0a067qty7 , zoone-a0a067rk35 , zoone-a0a067rk64 , zoone-a0a067rj74 , zoone-a0a067rp97 , zoone-a0a067rjm1

Title : Structure of the type VI secretion phospholipase effector Tle1 provides insight into its hydrolysis and membrane targeting - Hu_2014_Acta.Crystallogr.D.Biol.Crystallogr_70_2175
Author(s) : Hu H , Zhang H , Gao Z , Wang D , Liu G , Xu J , Lan K , Dong Y
Ref : Acta Crystallographica D Biol Crystallogr , 70 :2175 , 2014
Abstract : A diverse superfamily of phospholipases consisting of the type VI lipase effectors Tle1-Tle5 secreted by the bacterial type VI secretion system (T6SS) have recently been identified as antibacterial effectors that hydrolyze membrane phospholipids. These effectors show no significant homology to known lipases, and their mechanism of membrane targeting and hydrolysis of phospholipids remains unknown. Here, the crystal structure of Tle1 ( approximately 96.5 kDa) from Pseudomonas aeruginosa refined to 2.0 A resolution is reported, representing the first structure of this superfamily. Its overall structure can be divided into two distinct parts, the phospholipase catalytic module and the putative membrane-anchoring module; this arrangement has not previously been observed in known lipase structures. The phospholipase catalytic module has a canonical alpha/beta-hydrolase fold and mutation of any residue in the Ser-Asp-His catalytic triad abolishes its toxicity. The putative membrane-anchoring module adopts an open conformation composed of three amphipathic domains, and its partial folds are similar to those of several periplasmic or membrane proteins. A cell-toxicity assay revealed that the putative membrane-anchoring module is critical to Tle1 antibacterial activity. A molecular-dynamics (MD) simulation system in which the putative membrane-anchoring module embedded into a bilayer was stable over 50 ns. These structure-function studies provide insight into the hydrolysis and membrane-targeting process of the unique phospholipase Tle1.
ESTHER : Hu_2014_Acta.Crystallogr.D.Biol.Crystallogr_70_2175
PubMedSearch : Hu_2014_Acta.Crystallogr.D.Biol.Crystallogr_70_2175
PubMedID: 25084336
Gene_locus related to this paper: pseae-q9hyv3

Title : The genome of the clonal raider ant Cerapachys biroi - Oxley_2014_Curr.Biol_24_451
Author(s) : Oxley PR , Ji L , Fetter-Pruneda I , McKenzie SK , Li C , Hu H , Zhang G , Kronauer DJ
Ref : Current Biology , 24 :451 , 2014
Abstract : Social insects are important models for social evolution and behavior. However, in many species, experimental control over important factors that regulate division of labor, such as genotype and age, is limited. Furthermore, most species have fixed queen and worker castes, making it difficult to establish causality between the molecular mechanisms that underlie reproductive division of labor, the hallmark of insect societies. Here we present the genome of the queenless clonal raider ant Cerapachys biroi, a powerful new study system that does not suffer from these constraints. Using cytology and RAD-seq, we show that C. biroi reproduces via automixis with central fusion and that heterozygosity is lost extremely slowly. As a consequence, nestmates are almost clonally related (r = 0.996). Workers in C. biroi colonies synchronously alternate between reproduction and brood care, and young workers eclose in synchronized cohorts. We show that genes associated with division of labor in other social insects are conserved in C. biroi and dynamically regulated during the colony cycle. With unparalleled experimental control over an individual's genotype and age, and the ability to induce reproduction and brood care, C. biroi has great potential to illuminate the molecular regulation of division of labor.
ESTHER : Oxley_2014_Curr.Biol_24_451
PubMedSearch : Oxley_2014_Curr.Biol_24_451
PubMedID: 24508170
Gene_locus related to this paper: solin-e9ige7 , cerbi-a0a026whr6 , cerbi-a0a026wk96 , cerbi-a0a026vw30 , cerbi-a0a026wfw0 , cerbi-a0a026w5f6 , cerbi-a0a026wt53 , cerbi-a0a026vug0 , cerbi-a0a026x3b8 , cerbi-a0a026we54 , cerbi-a0a026wla0 , cerbi-a0a026wis7 , cerbi-a0a026wrn0 , cerbi-a0a026wi21 , cerbi-a0a026wec0 , cerbi-a0a026wvz8 , cerbi-a0a026w8e0 , oocbi-a0a026wtb1 , oocbi-a0a026wvq6 , oocbi-a0a026w0p3 , oocbi-a0a026w634

Title : Genomic diversity and evolution of the head crest in the rock pigeon - Shapiro_2013_Science_339_1063
Author(s) : Shapiro MD , Kronenberg Z , Li C , Domyan ET , Pan H , Campbell M , Tan H , Huff CD , Hu H , Vickrey AI , Nielsen SC , Stringham SA , Willerslev E , Gilbert MT , Yandell M , Zhang G , Wang J
Ref : Science , 339 :1063 , 2013
Abstract : The geographic origins of breeds and the genetic basis of variation within the widely distributed and phenotypically diverse domestic rock pigeon (Columba livia) remain largely unknown. We generated a rock pigeon reference genome and additional genome sequences representing domestic and feral populations. We found evidence for the origins of major breed groups in the Middle East and contributions from a racing breed to North American feral populations. We identified the gene EphB2 as a strong candidate for the derived head crest phenotype shared by numerous breeds, an important trait in mate selection in many avian species. We also found evidence that this trait evolved just once and spread throughout the species, and that the crest originates early in development by the localized molecular reversal of feather bud polarity.
ESTHER : Shapiro_2013_Science_339_1063
PubMedSearch : Shapiro_2013_Science_339_1063
PubMedID: 23371554
Gene_locus related to this paper: colli-r7vnu6 , colli-r7vv16 , colli-a0a2i0m6q6 , colli-a0a2i0mey7 , colli-a0a2i0mey8 , colli-a0a2i0mez3 , colli-a0a2i0ms89 , colli-a0a160dr48 , colli-a0a2i0m6c1 , colli-a0a2i0lic2 , colli-a0a2i0mlj2 , colli-r7vwj5 , nipni-a0a091w0t8 , fical-u3jnn0 , colli-a0a2i0mwb1 , colli-a0a2i0mwb4 , colli-a0a2i0mwd0

Title : Tin (IV) chloride-promoted one-pot synthesis of novel tacrine analogues - Hu_2011_Molecules_16_1878
Author(s) : Hu H , Song L , Fang Q , Zheng J , Meng Z , Luo Y
Ref : Molecules , 16 :1878 , 2011
Abstract : A facile synthesis of potential acetylcholinesterase (AChE) inhibitors, the tacrine analogues 3a-p, has been accomplished by direct cyclocondensation of 1-aryl-4-cyano-5-aminopyrazole with beta-ketoesters using tin(IV) chloride as catalyst. The structures of all the compounds have been confirmed by IR, (1)H- and (1)(3)C-NMR.
ESTHER : Hu_2011_Molecules_16_1878
PubMedSearch : Hu_2011_Molecules_16_1878
PubMedID: 21343890

Title : The genome of the leaf-cutting ant Acromyrmex echinatior suggests key adaptations to advanced social life and fungus farming - Nygaard_2011_Genome.Res_21_1339
Author(s) : Nygaard S , Zhang G , Schiott M , Li C , Wurm Y , Hu H , Zhou J , Ji L , Qiu F , Rasmussen M , Pan H , Hauser F , Krogh A , Grimmelikhuijzen CJ , Wang J , Boomsma JJ
Ref : Genome Res , 21 :1339 , 2011
Abstract : We present a high-quality (>100x depth) Illumina genome sequence of the leaf-cutting ant Acromyrmex echinatior, a model species for symbiosis and reproductive conflict studies. We compare this genome with three previously sequenced genomes of ants from different subfamilies and focus our analyses on aspects of the genome likely to be associated with known evolutionary changes. The first is the specialized fungal diet of A. echinatior, where we find gene loss in the ant's arginine synthesis pathway, loss of detoxification genes, and expansion of a group of peptidase proteins. One of these is a unique ant-derived contribution to the fecal fluid, which otherwise consists of "garden manuring" fungal enzymes that are unaffected by ant digestion. The second is multiple mating of queens and ejaculate competition, which may be associated with a greatly expanded nardilysin-like peptidase gene family. The third is sex determination, where we could identify only a single homolog of the feminizer gene. As other ants and the honeybee have duplications of this gene, we hypothesize that this may partly explain the frequent production of diploid male larvae in A. echinatior. The fourth is the evolution of eusociality, where we find a highly conserved ant-specific profile of neuropeptide genes that may be related to caste determination. These first analyses of the A. echinatior genome indicate that considerable genetic changes are likely to have accompanied the transition from hunter-gathering to agricultural food production 50 million years ago, and the transition from single to multiple queen mating 10 million years ago.
ESTHER : Nygaard_2011_Genome.Res_21_1339
PubMedSearch : Nygaard_2011_Genome.Res_21_1339
PubMedID: 21719571
Gene_locus related to this paper: acrec-f4we58 , acrec-f4wfr0 , acrec-f4wwr9 , acrec-f4x396 , acrec-f4wlq1 , acrec-f4wk97 , acrec-f4wdb2 , acrec-f4wdb3 , acrec-f4x1t2

Title : Unique regulation of adipose triglyceride lipase (ATGL) by perilipin 5, a lipid droplet-associated protein - Wang_2011_J.Biol.Chem_286_15707
Author(s) : Wang H , Bell M , Sreenivasan U , Hu H , Liu J , Dalen K , Londos C , Yamaguchi T , Rizzo MA , Coleman R , Gong D , Brasaemle D , Sztalryd C
Ref : Journal of Biological Chemistry , 286 :15707 , 2011
Abstract : Lipolysis is a critical metabolic pathway contributing to energy homeostasis through degradation of triacylglycerides stored in lipid droplets (LDs), releasing fatty acids. Neutral lipid lipases act at the oil/water interface. In mammalian cells, LD surfaces are coated with one or more members of the perilipin protein family, which serve important functions in regulating lipolysis. We investigated mechanisms by which three perilipin proteins control lipolysis by adipocyte triglyceride lipase (ATGL), a key lipase in adipocytes and non-adipose cells. Using a cell culture model, we examined interactions of ATGL and its co-lipase CGI-58 with perilipin 1 (perilipin A), perilipin 2 (adipose differentiation-related protein), and perilipin 5 (LSDP5) using multiple techniques as follows: anisotropy Forster resonance energy transfer, co-immunoprecipitation, [(32)P]orthophosphate radiolabeling, and measurement of lipolysis. The results show that ATGL interacts with CGI-58 and perilipin 5; the latter is selectively expressed in oxidative tissues. Both proteins independently recruited ATGL to the LD surface, but with opposite effects; interaction of ATGL with CGI-58 increased lipolysis, whereas interaction of ATGL with perilipin 5 decreased lipolysis. In contrast, neither perilipin 1 nor 2 interacted directly with ATGL. Activation of protein kinase A (PKA) increased [(32)P]orthophosphate incorporation into perilipin 5 by 2-fold, whereas neither ATGL nor CGI-58 was labeled under the incubation conditions. Cells expressing both ectopic perilipin 5 and ATGL showed a 3-fold increase in lipolysis following activation of PKA. Our studies establish perilipin 5 as a novel ATGL partner and provide evidence that the protein composition of perilipins at the LD surface regulates lipolytic activity of ATGL.
ESTHER : Wang_2011_J.Biol.Chem_286_15707
PubMedSearch : Wang_2011_J.Biol.Chem_286_15707
PubMedID: 21393244

Title : The genome sequence of the leaf-cutter ant Atta cephalotes reveals insights into its obligate symbiotic lifestyle - Suen_2011_PLoS.Genet_7_e1002007
Author(s) : Suen G , Teiling C , Li L , Holt C , Abouheif E , Bornberg-Bauer E , Bouffard P , Caldera EJ , Cash E , Cavanaugh A , Denas O , Elhaik E , Fave MJ , Gadau J , Gibson JD , Graur D , Grubbs KJ , Hagen DE , Harkins TT , Helmkampf M , Hu H , Johnson BR , Kim J , Marsh SE , Moeller JA , Munoz-Torres MC , Murphy MC , Naughton MC , Nigam S , Overson R , Rajakumar R , Reese JT , Scott JJ , Smith CR , Tao S , Tsutsui ND , Viljakainen L , Wissler L , Yandell MD , Zimmer F , Taylor J , Slater SC , Clifton SW , Warren WC , Elsik CG , Smith CD , Weinstock GM , Gerardo NM , Currie CR
Ref : PLoS Genet , 7 :e1002007 , 2011
Abstract : Leaf-cutter ants are one of the most important herbivorous insects in the Neotropics, harvesting vast quantities of fresh leaf material. The ants use leaves to cultivate a fungus that serves as the colony's primary food source. This obligate ant-fungus mutualism is one of the few occurrences of farming by non-humans and likely facilitated the formation of their massive colonies. Mature leaf-cutter ant colonies contain millions of workers ranging in size from small garden tenders to large soldiers, resulting in one of the most complex polymorphic caste systems within ants. To begin uncovering the genomic underpinnings of this system, we sequenced the genome of Atta cephalotes using 454 pyrosequencing. One prediction from this ant's lifestyle is that it has undergone genetic modifications that reflect its obligate dependence on the fungus for nutrients. Analysis of this genome sequence is consistent with this hypothesis, as we find evidence for reductions in genes related to nutrient acquisition. These include extensive reductions in serine proteases (which are likely unnecessary because proteolysis is not a primary mechanism used to process nutrients obtained from the fungus), a loss of genes involved in arginine biosynthesis (suggesting that this amino acid is obtained from the fungus), and the absence of a hexamerin (which sequesters amino acids during larval development in other insects). Following recent reports of genome sequences from other insects that engage in symbioses with beneficial microbes, the A. cephalotes genome provides new insights into the symbiotic lifestyle of this ant and advances our understanding of host-microbe symbioses.
ESTHER : Suen_2011_PLoS.Genet_7_e1002007
PubMedSearch : Suen_2011_PLoS.Genet_7_e1002007
PubMedID: 21347285
Gene_locus related to this paper: acrec-f4w848 , acrec-f4wah1 , acrec-f4wai9 , acrec-f4wda7 , acrec-f4wfh7 , acrec-f4wk54 , acrec-f4wng2 , acrec-f4wpb7 , acrec-f4wpb8 , acrec-f4wpb9 , acrec-f4x2j7 , acrec-f4x3l5 , acrec-f4x6y6 , acrec-f4x7w5 , acrec-f4x7w6 , acrec-f4x378 , acrec-f4x808 , attce-h9hc46 , solin-e9ige7 , attce-w4wts9 , attce-w4x506 , attce-w4vya2 , attce-w4vyi5 , attce-w4wib4 , attce-w4wkj3 , attce-w4x3s7 , attce-w4x3u4 , attce-w4wu92 , attce-w4w1m9 , attce-w4x2m8 , attce-w4w776 , attce-w4x1t1 , attce-a0a158nl98 , attce-a0a158nmi0 , attce-a0a158nqx5 , attce-a0a158nr47.2 , attce-a0a158ns84 , attce-a0a158nvq4 , attce-a0a158nij4 , attce-a0a158nhg2 , attce-a0a158nhn7 , attce-a0a158nbh6 , attce-a0a158ne04 , attce-a0a158nyf0

Title : Genome sequencing and comparison of two nonhuman primate animal models, the cynomolgus and Chinese rhesus macaques - Yan_2011_Nat.Biotechnol_29_1019
Author(s) : Yan G , Zhang G , Fang X , Zhang Y , Li C , Ling F , Cooper DN , Li Q , Li Y , van Gool AJ , Du H , Chen J , Chen R , Zhang P , Huang Z , Thompson JR , Meng Y , Bai Y , Wang J , Zhuo M , Wang T , Huang Y , Wei L , Li J , Wang Z , Hu H , Yang P , Le L , Stenson PD , Li B , Liu X , Ball EV , An N , Huang Q , Fan W , Zhang X , Wang W , Katze MG , Su B , Nielsen R , Yang H , Wang X
Ref : Nat Biotechnol , 29 :1019 , 2011
Abstract : The nonhuman primates most commonly used in medical research are from the genus Macaca. To better understand the genetic differences between these animal models, we present high-quality draft genome sequences from two macaque species, the cynomolgus/crab-eating macaque and the Chinese rhesus macaque. Comparison with the previously sequenced Indian rhesus macaque reveals that all three macaques maintain abundant genetic heterogeneity, including millions of single-nucleotide substitutions and many insertions, deletions and gross chromosomal rearrangements. By assessing genetic regions with reduced variability, we identify genes in each macaque species that may have experienced positive selection. Genetic divergence patterns suggest that the cynomolgus macaque genome has been shaped by introgression after hybridization with the Chinese rhesus macaque. Macaque genes display a high degree of sequence similarity with human disease gene orthologs and drug targets. However, we identify several putatively dysfunctional genetic differences between the three macaque species, which may explain functional differences between them previously observed in clinical studies.
ESTHER : Yan_2011_Nat.Biotechnol_29_1019
PubMedSearch : Yan_2011_Nat.Biotechnol_29_1019
PubMedID: 22002653
Gene_locus related to this paper: macfa-BCHE , macfa-g7nzc0 , macfa-g7nze2 , macfa-g7p4b9 , macfa-g7pa87 , macfa-g7pd01 , macfa-g7q259 , macfa-3neur , macfa-g8f585 , macfa-KANSL3 , macfa-q4r8p0 , macfa-SPG21 , macfa-TEX30 , macmu-3neur , macmu-ACHE , macmu-BCHE , macmu-f6sz31 , macmu-f6the6 , macmu-f6zkq5 , macmu-f7buk8 , macmu-f7cfi8 , macmu-f7flv1 , macmu-f7ggk1 , macmu-f7hir7 , macmu-g7n054 , macmu-g7npb8 , macmu-g7nq39 , macmu-KANSL3 , macmu-TEX30 , macfa-g7pgg6 , macmu-g7n4x3 , macfa-g7nzx2 , macfa-g8f4f7 , macmu-f7ba84 , macfa-g7psx7 , macmu-h9er02 , macfa-g8f3k0 , macfa-a0a2k5w1n7 , macmu-g7mxj6 , macfa-g7pbk1 , macfa-a0a2k5urk5 , macfa-a0a2k5wye4 , macfa-g7pe14 , macmu-f7hkw9 , macmu-f7hm08 , macmu-g7mke4 , macfa-g7nxn9 , macmu-a0a1d5rh04 , macmu-h9fud6 , macfa-g8f3e1 , macfa-i7gcw6 , macmu-f6qwx1 , macmu-f7h4t2 , macfa-a0a2k5wkd0 , macfa-a0a2k5v7v4 , macfa-g7p7y3 , macfa-a0a2k5uqq3 , macmu-i2cu80 , macfa-g8f5i1 , macmu-f7h550 , macmu-f7gkb9 , macfa-a0a2k5tui1

Title : Exercise benefits cardiovascular health in hyperlipidemia rats correlating with changes of the cardiac vagus nerve - Wang_2010_Eur.J.Appl.Physiol_108_459
Author(s) : Wang YH , Hu H , Wang SP , Tian ZJ , Zhang QJ , Li QX , Li YY , Yu XJ , Sun L , Li DL , Jia B , Liu BH , Zang WJ
Ref : Eur J Appl Physiol , 108 :459 , 2010
Abstract : The role of exercise training on hemodynamic parameters, blood lipid profiles, inflammatory cytokines, cholinesterase-positive nerves and muscarinic cholinergic (M(2)) receptors expression in the heart was investigated in Sprague-Dawley male rats with hyperlipidemia (HL). The rats were subjected to a high-fat diet and exercise training for 8 weeks, and then the hemodynamic parameters, the profiles of blood lipid and inflammatory cytokines, and the expression of cholinesterase-positive nerves and M(2) receptors were measured. HL rats displayed cardiac dysfunction, dysregulation of inflammatory cytokines, and decreased cholinesterase-positive nerves and M(2) receptors expression. The combination of hyperlipidemia with exercise training (AT) restored the profiles of blood lipids and the levels of inflammatory cytokines. In addition, AT and HL + AT improved cardiac function with increasing cholinesterase-positive nerves and M(2) receptors expression. Overall, these data show that the increased expression of cholinesterase-positive nerves and M(2) receptors in the heart is partially responsible for the benefits of exercise training on cardiac function in hyperlipidemia rats.
ESTHER : Wang_2010_Eur.J.Appl.Physiol_108_459
PubMedSearch : Wang_2010_Eur.J.Appl.Physiol_108_459
PubMedID: 19830451

Title : A novel chemiluminescence assay of organophosphorous pesticide quinalphos residue in vegetable with luminol detection - Hu_2010_Chem.Cent.J_4_13
Author(s) : Hu H , Liu X , Jiang F , Yao X , Cui X
Ref : Chem Cent J , 4 :13 , 2010
Abstract : BACKGROUND Organophosphorous pesticides are the most popular pesticides used in agriculture. As acetylcholinesterase inhibitors, organophosphorous pesticides are toxic organic chemicals. The control and detection of organophosphorous pesticide residue in food, water, and environment therefore plays a very important role in maintaining physical health. A sensitive, rapid, simple chemiluminescence(CL) method has been developed for the determination of quinalphos based on the reaction of quinalphos with luminol-H2O2 in an alkaline medium. The method has been applied to detection of quinalphos in vegetable samples with satisfactory results. RESULTS: The CL method for the determination of organophosphorous pesticide quinalphos is based on the phenomenon that quinalphos can apparently enhance the CL intensity of the luminol-H2O2 system. The optimal conditions were: luminol concentration 5.0 x 10-4 mol/L, H2O2 concentration 0.05 mol/L.pH value 13. In order to restrain the interference from metal ions, 1.0 x 10-3 mol/L of EDTA was added to the luminol solution. The possible mechanism was proposed. CONCLUSION: Under the optimum reaction conditions, CL was linear with the concentration of quinalphos in the range of 0.02 mug/mL -1.0 mug/mL and the detection limit was 0.0055 mug/mL (3sigma). This method has been successfully applied to the detection of quinalphos in vegetable samples. According to the experimental data, the average recoveries for quinalphos in cherry tomato and green pepper 97.20% and 90.13%. Meanwhile, the possible mechanism was proposed.
ESTHER : Hu_2010_Chem.Cent.J_4_13
PubMedSearch : Hu_2010_Chem.Cent.J_4_13
PubMedID: 20576096

Title : Association of lead exposure with survival in amyotrophic lateral sclerosis - Kamel_2008_Environ.Health.Perspect_116_943
Author(s) : Kamel F , Umbach DM , Stallone L , Richards M , Hu H , Sandler DP
Ref : Environmental Health Perspectives , 116 :943 , 2008
Abstract : BACKGROUND: Reasons for the variability in survival among ALS cases are unknown but may include exposure to environmental neurotoxicants. OBJECTIVES: We aimed to determine whether lead exposure, assessed by measuring blood and bone lead levels, is associated with survival in amyotrophic lateral sclerosis (ALS).
METHODS: We evaluated the relationship of lead exposure to ALS survival in 110 cases from a case-control study conducted in New England in 1993-1996 that included measurements of blood and bone lead. We retrieved information on date and cause of death through 31 December 2003 from the National Death Index Plus and the Social Security Administration Death Index. We evaluated the relationship of survival to lead exposure using Cox proportional hazard analysis, with adjustment for age, sex, and smoking.
RESULTS: We found mortality data for 100 of 110 cases; 93 of 100 death certificates mentioned ALS. Median survival from diagnosis to death was 28 months. Shorter survival was associated with older age at diagnosis, female sex, bulbar onset, shorter interval between symptom onset and diagnosis, and reduced lung function. Shorter survival from diagnosis to death had a weak inverse association with blood lead (hazard ratio = 0.9; 95% confidence interval, 0.8-1.0) and a stronger inverse association with patella lead (0.5; 0.2-1.0) and tibia lead (0.3; 0.1-0.7); similar results were found for survival from symptom onset to death.
CONCLUSIONS: These results suggest that lead exposure is associated with longer survival in ALS cases and, if confirmed, may shed light on mechanisms involved in disease progression.
ESTHER : Kamel_2008_Environ.Health.Perspect_116_943
PubMedSearch : Kamel_2008_Environ.Health.Perspect_116_943
PubMedID: 18629318

Title : VEGF promoter haplotype and amyotrophic lateral sclerosis (ALS) - Terry_2004_J.Neurogenet_18_429
Author(s) : Terry PD , Kamel F , Umbach DM , Lehman TA , Hu H , Sandler DP , Taylor JA
Ref : J Neurogenet , 18 :429 , 2004
Abstract : Vascular endothelial growth factor (VEGF) is a cytokine essential for angiogenesis. A recent study found that haplotypes, determined by three SNPs (-2,578C/A, - 1,154 G/A, and - 634G/C) in the VEGF upstream promoter/leader sequence, were associated with risk of amyotrophic lateral sclerosis(ALS). We used samples and data from a case-control study to examine the relation of ALS to VEGF haplotype. Genotypes at each of the three polymorphic sites were determined using allele-specific primer extension reactions followed by MALDI-TOF. We found a 3-fold increased risk among individuals homozygous for the AAG or AGG haplotypes (95%CI = 0.7 - 13.4), consistent with the findings of the previous study. Given the wide confidence interval, our findings should be interpreted cautiously.
ESTHER : Terry_2004_J.Neurogenet_18_429
PubMedSearch : Terry_2004_J.Neurogenet_18_429
PubMedID: 15763997

Title : Identification and characterization of pltZ, a gene involved in the repression of pyoluteorin biosynthesis in Pseudomonas sp. M18 - Huang_2004_FEMS.Microbiol.Lett_232_197
Author(s) : Huang X , Zhu D , Ge Y , Hu H , Zhang X , Xu Y
Ref : FEMS Microbiology Letters , 232 :197 , 2004
Abstract : A new regulator gene named pltZ, which is located downstream of the plt gene cluster in the genome of Pseudomonas sp. M18, was identified, sequenced and characterized in this report. The deduced amino acid sequence of PltZ shares significant homology with other bacterial regulators in the TetR family. The chromosomal pltZ disruption mutant gave rise to 4.4-fold enhancement of pyoluteorin biosynthesis but did not exert significant influence on the accumulation of phenazine-1-carboxylic acid compared with the wild-type M18. The negative regulation of pltZ on pyoluteorin biosynthesis was further confirmed by multiplied pltZ gene dosage experiments and pltA'-'lacZ translational fusion analyses.
ESTHER : Huang_2004_FEMS.Microbiol.Lett_232_197
PubMedSearch : Huang_2004_FEMS.Microbiol.Lett_232_197
PubMedID: 15033239
Gene_locus related to this paper: 9psed-q6tlj4

Title : Sequence and analysis of rice chromosome 4 - Feng_2002_Nature_420_316
Author(s) : Feng Q , Zhang Y , Hao P , Wang S , Fu G , Huang Y , Li Y , Zhu J , Liu Y , Hu X , Jia P , Zhao Q , Ying K , Yu S , Tang Y , Weng Q , Zhang L , Lu Y , Mu J , Zhang LS , Yu Z , Fan D , Liu X , Lu T , Li C , Wu Y , Sun T , Lei H , Li T , Hu H , Guan J , Wu M , Zhang R , Zhou B , Chen Z , Chen L , Jin Z , Wang R , Yin H , Cai Z , Ren S , Lv G , Gu W , Zhu G , Tu Y , Jia J , Chen J , Kang H , Chen X , Shao C , Sun Y , Hu Q , Zhang X , Zhang W , Wang L , Ding C , Sheng H , Gu J , Chen S , Ni L , Zhu F , Chen W , Lan L , Lai Y , Cheng Z , Gu M , Jiang J , Li J , Hong G , Xue Y , Han B
Ref : Nature , 420 :316 , 2002
Abstract : Rice is the principal food for over half of the population of the world. With its genome size of 430 megabase pairs (Mb), the cultivated rice species Oryza sativa is a model plant for genome research. Here we report the sequence analysis of chromosome 4 of O. sativa, one of the first two rice chromosomes to be sequenced completely. The finished sequence spans 34.6 Mb and represents 97.3% of the chromosome. In addition, we report the longest known sequence for a plant centromere, a completely sequenced contig of 1.16 Mb corresponding to the centromeric region of chromosome 4. We predict 4,658 protein coding genes and 70 transfer RNA genes. A total of 1,681 predicted genes match available unique rice expressed sequence tags. Transposable elements have a pronounced bias towards the euchromatic regions, indicating a close correlation of their distributions to genes along the chromosome. Comparative genome analysis between cultivated rice subspecies shows that there is an overall syntenic relationship between the chromosomes and divergence at the level of single-nucleotide polymorphisms and insertions and deletions. By contrast, there is little conservation in gene order between rice and Arabidopsis.
ESTHER : Feng_2002_Nature_420_316
PubMedSearch : Feng_2002_Nature_420_316
PubMedID: 12447439
Gene_locus related to this paper: orysa-Q7XTC5 , orysa-Q7F959 , orysa-q7f9i3 , orysa-q7x7y5 , orysa-q7xkj9 , orysa-q7xr62 , orysa-q7xr63 , orysa-q7xr64 , orysa-q7xsg1 , orysa-q7xsq2 , orysa-Q7XTM8 , orysa-q7xts6 , orysa-q7xue7 , orysa-q7xv53 , orysa-Q7XVB5 , orysa-Q7XVG5 , orysj-q0jaf0 , orysj-q7f8x1