xanau-halo1
Xanthobacter autotrophicus, Xanthobacter flavus, Ancylobacter aquaticus, haloalkane dehalogenase DhlA
Comment
Other strains: Xanthobacter autotrophicus GJ10, Xanthobacter flavus\; Ancylobacter aquaticus || Q6Q3H0 AY561847 for Xanthobacter flavus identical
Relationship
Family : Haloalkane_dehalogenase-HLD1
Block : X
Position in NCBI Life Tree : Xanthobacter autotrophicus
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Proteobacteria N E > Alphaproteobacteria N E > Rhizobiales N E > Xanthobacteraceae N E > Xanthobacter N E > Xanthobacter autotrophicus N EMolecular evidence
No mutation
1B6G - Haloalkane dehalogenase at Ph 5.0 containing chloride
1BE0 - Xanthobacter autotrophicus haloalkane dehalogenase (pH 5.0) +acetic acid
1BEE - Xanthobacter autotrophicus haloalkane dehalogenase W175Y mutant
1BEZ - Xanthobacter autotrophicus haloalkane dehalogenase W175Y mutant (pH 5.0)
1CIJ - Haloalkane dehalogenase soaked with high concentration of bromide
1EDB - Xanthobacter autotrophicus haloalkane dehalogenase at PH 6.2
1EDD - Xanthobacter autotrophicus haloalkane dehalogenase at PH 8
1EDE - Xanthobacter autotrophicus haloalkane dehalogenase at PH 8.2
1HDE - Xanthobacter autotrophicus haloalkane dehalogenase F172W mutant
2DHC - Xanthobacter autotrophicus haloalkane dehalogenase + 1,2-dichloroethane at 4 Deg celcius
2DHD - Xanthobacter autotrophicus haloalkane dehalogenase + 1,2-dichloroethane at room temp
2DHE - Xanthobacter autotrophicus haloalkane dehalogenase at room temp PH 6
2EDA - Xanthobacter autotrophicus haloalkane dehalogenase + iodine
2EDC - Xanthobacter autotrophicus haloalkane dehalogenase
2HAD - Xanthobacter autotrophicus haloalkane dehalogenase
2PKY - The Effect of Deuteration on Protein Structure A High Resolution Comparison of Hydrogenous and Perdeuterated Haloalkane Dehalogenase
2YXP - The Effect of Deuteration on Protein Structure A High Resolution Comparison of Hydrogenous and Perdeuterated Haloalkane Dehalogenase
No kinetic
No disease
(Bromomethyl)cylcohexane
1,2,3-Tribromopropane
1,2-Dibromo-3-Chloropropane
1,2-dibromoethane
1,2-dibromopropane
1,2-dichloroethane
1,3-dibromopropane
1,3-dichloropropane
1,3-diiodopropane
1,5-dichloropentane
1-Bromo-2-chloroethane
1-Bromo-3-Chloropropane
1-Bromobutane
1-Chloro-2-(2-chloroethoxy)ethane
1-Chlorobutane
1-Chlorohexane
1-Iodobutane
1-Iodohexane
1-Iodopropane
1-bromohexane
2,3-Dichloroprop-1-ene
2-Bromo-1-Chloropropane
2-Iodobutane
3-Chloro-2-methylprop-1-ene
4-Bromobutanenitrile
Bromocyclohexane
Chlorocyclohexane
Chlorocyclopentane
Mustard-gas
No inhibitor
Sequence
Peptide
MINAIRTPDQ RFSNLDQYPF SPNYLDDLPG YPGLRAHYLD EGNSDAEDVF LCLHGEPTWS YLYRKMIPVF AESGARVIAP DFFGFGKSDK PVDEEDYTFE FHRNFLLALI ERLDLRNITL VVQDWGGFLG LTLPMADPSR FKRLIIMNAC LMTDPVTQPA FSAFVTQPAD GFTAWKYDLV TPSDLRLDQF MKRWAPTLTE AEASAYAAPF PDTSYQAGVR KFPKMVAQRD QACIDISTEA ISFWQNDWNG QTFMAIGMKD KLLGPDVMYP MKALINGCPE PLEIADAGHF VQEFGEQVAR EALKHFAETE
References (26)
| Title : Biochemical and Computational Characterization of Haloalkane Dehalogenase Variants Designed by Generative AI: Accelerating the S(N)2 Step - Gelfand_2025_J.Am.Chem.Soc_147_2747 |
| Author(s) : Gelfand N , Orel V , Cui W , Damborsky J , Li C , Prokop Z , Xie WJ , Warshel A |
| Ref : Journal of the American Chemical Society , 147 :2747 , 2025 |
| Abstract : |
| PubMedSearch : Gelfand_2025_J.Am.Chem.Soc_147_2747 |
| PubMedID: 39792627 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Exploring the challenges of computational enzyme design by rebuilding the active site of a dehalogenase - Jindal_2019_Proc.Natl.Acad.Sci.U.S.A_116_389 |
| Author(s) : Jindal G , Slanska K , Kolev V , Damborsky J , Prokop Z , Warshel A |
| Ref : Proc Natl Acad Sci U S A , 116 :389 , 2019 |
| Abstract : |
| PubMedSearch : Jindal_2019_Proc.Natl.Acad.Sci.U.S.A_116_389 |
| PubMedID: 30587585 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : One-step orientated immobilization of nanobodies and its application for immunoglobulin purification - Fu_2019_J.Chromatogr.A_1603_15 |
| Author(s) : Fu J , Li J , Wang W , Wu H , Zhou P , Li Y , He Q , Tu Z |
| Ref : Journal of Chromatography A , 1603 :15 , 2019 |
| Abstract : |
| PubMedSearch : Fu_2019_J.Chromatogr.A_1603_15 |
| PubMedID: 31213362 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Bacillus subtilis Spore Surface Display of Haloalkane Dehalogenase DhaA - Wang_2019_Curr.Microbiol_76_1161 |
| Author(s) : Wang F , Song T , Jiang H , Pei C , Huang Q , Xi H |
| Ref : Curr Microbiol , 76 :1161 , 2019 |
| Abstract : |
| PubMedSearch : Wang_2019_Curr.Microbiol_76_1161 |
| PubMedID: 31278426 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : A Novel Mini Protein Design of Haloalkane Dehalogenase - Daud_2019_Mol.Biotechnol_61_477 |
| Author(s) : Daud NH , Leow ATC , Oslan SN , Salleh AB |
| Ref : Mol Biotechnol , 61 :477 , 2019 |
| Abstract : |
| PubMedSearch : Daud_2019_Mol.Biotechnol_61_477 |
| PubMedID: 30919327 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Conformational changes allow processing of bulky substrates by a haloalkane dehalogenase with a small and buried active site - Kokkonen_2018_J.Biol.Chem_293_11505 |
| Author(s) : Kokkonen P , Bednar D , Dockalova V , Prokop Z , Damborsky J |
| Ref : Journal of Biological Chemistry , 293 :11505 , 2018 |
| Abstract : |
| PubMedSearch : Kokkonen_2018_J.Biol.Chem_293_11505 |
| PubMedID: 29858243 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Substrate specificity of haloalkane dehalogenases - Koudelakova_2011_Biochem.J_435_345 |
| Author(s) : Koudelakova T , Chovancova E , Brezovsky J , Monincova M , Fortova A , Jarkovsky J , Damborsky J |
| Ref : Biochemical Journal , 435 :345 , 2011 |
| Abstract : |
| PubMedSearch : Koudelakova_2011_Biochem.J_435_345 |
| PubMedID: 21294712 |
| Gene_locus related to this paper: agrtu-DHAA , brael-e2rv62 , braja-dhaa , myctu-linb , myctu-Rv1833c , rhoba-DHLA , rhoso-halo1 , sphpi-linb , xanau-halo1 |
| Title : The effect of deuteration on protein structure: a high-resolution comparison of hydrogenous and perdeuterated haloalkane dehalogenase - Liu_2007_Acta.Crystallogr.D.Biol.Crystallogr_63_1000 |
| Author(s) : Liu X , Hanson BL , Langan P , Viola RE |
| Ref : Acta Crystallographica D Biol Crystallogr , 63 :1000 , 2007 |
| Abstract : |
| PubMedSearch : Liu_2007_Acta.Crystallogr.D.Biol.Crystallogr_63_1000 |
| PubMedID: 17704569 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Exploring the binding sites of the haloalkane dehalogenase DhlA from Xanthobacter autotrophicus GJ10 - Silberstein_2007_Biochemistry_46_9239 |
| Author(s) : Silberstein M , Damborsky J , Vajda S |
| Ref : Biochemistry , 46 :9239 , 2007 |
| Abstract : |
| PubMedSearch : Silberstein_2007_Biochemistry_46_9239 |
| PubMedID: 17645312 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Comparative binding energy analysis of the substrate specificity of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 - Kmunicek_2001_Biochemistry_40_8905 |
| Author(s) : Kmunicek J , Luengo S , Gago F , Ortiz AR , Wade RC , Damborsky J |
| Ref : Biochemistry , 40 :8905 , 2001 |
| Abstract : |
| PubMedSearch : Kmunicek_2001_Biochemistry_40_8905 |
| PubMedID: 11467952 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Crystallographic and kinetic evidence of a collision complex formed during halide import in haloalkane dehalogenase - Pikkemaat_1999_Biochemistry_38_12052 |
| Author(s) : Pikkemaat MG , Ridder IS , Rozeboom HJ , Kalk KH , Dijkstra BW , Janssen DB |
| Ref : Biochemistry , 38 :12052 , 1999 |
| Abstract : |
| PubMedSearch : Pikkemaat_1999_Biochemistry_38_12052 |
| PubMedID: 10508409 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 refined at 1.15 A resolution - Ridder_1999_Acta.Crystallogr.D.Biol.Crystallogr_55_1273 |
| Author(s) : Ridder IS , Rozeboom HJ , Dijkstra BW |
| Ref : Acta Crystallographica D Biol Crystallogr , 55 :1273 , 1999 |
| Abstract : |
| PubMedSearch : Ridder_1999_Acta.Crystallogr.D.Biol.Crystallogr_55_1273 |
| PubMedID: 10393294 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Kinetic analysis and X-ray structure of haloalkane dehalogenase with a modified halide-binding site - Krooshof_1998_Biochemistry_37_15013 |
| Author(s) : Krooshof GH , Ridder IS , Tepper AW , Vos GJ , Rozeboom HJ , Kalk KH , Dijkstra BW , Janssen DB |
| Ref : Biochemistry , 37 :15013 , 1998 |
| Abstract : |
| PubMedSearch : Krooshof_1998_Biochemistry_37_15013 |
| PubMedID: 9790663 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Kinetic characterization and X-ray structure of a mutant of haloalkane dehalogenase with higher catalytic activity and modified substrate range - Schanstra_1996_Biochemistry_35_13186 |
| Author(s) : Schanstra JP , Ridder IS , Heimeriks GJ , Rink R , Poelarends GJ , Kalk KH , Dijkstra BW , Janssen DB |
| Ref : Biochemistry , 35 :13186 , 1996 |
| Abstract : |
| PubMedSearch : Schanstra_1996_Biochemistry_35_13186 |
| PubMedID: 8855957 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Replacement of tryptophan residues in haloalkane dehalogenase reduces halide binding and catalytic activity - Kennes_1995_Eur.J.Biochem_228_403 |
| Author(s) : Kennes C , Pries F , Krooshof GH , Bokma E , Kingma J , Janssen DB |
| Ref : European Journal of Biochemistry , 228 :403 , 1995 |
| Abstract : |
| PubMedSearch : Kennes_1995_Eur.J.Biochem_228_403 |
| PubMedID: 7705355 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Histidine 289 is essential for hydrolysis of the alkyl-enzyme intermediate of haloalkane dehalogenase - Pries_1995_J.Biol.Chem_270_10405 |
| Author(s) : Pries F , Kingma J , Krooshof GH , Jeronimus-Stratingh CM , Bruins AP , Janssen DB |
| Ref : Journal of Biological Chemistry , 270 :10405 , 1995 |
| Abstract : |
| PubMedSearch : Pries_1995_J.Biol.Chem_270_10405 |
| PubMedID: 7737973 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Sequence analysis of the upstream region of dhlB, the gene encoding haloalkanoic acid dehalogenase of Xanthobacter autotrophicus GJ10 - van der Ploeg_1995_Biodegradation_6_257 |
| Author(s) : van der Ploeg J , Janssen DB |
| Ref : Biodegradation , 6 :257 , 1995 |
| Abstract : |
| PubMedSearch : van der Ploeg_1995_Biodegradation_6_257 |
| PubMedID: 7580000 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase - Verschueren_1993_Nature_363_693 |
| Author(s) : Verschueren KH , Seljee F , Rozeboom HJ , Kalk KH , Dijkstra BW |
| Ref : Nature , 363 :693 , 1993 |
| Abstract : |
| PubMedSearch : Verschueren_1993_Nature_363_693 |
| PubMedID: 8515812 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Crystallographic and fluorescence studies of the interaction of haloalkane dehalogenase with halide ions. Studies with halide compounds reveal a halide binding site in the active site - Verschueren_1993_Biochemistry_32_9031 |
| Author(s) : Verschueren KH , Kingma J , Rozeboom HJ , Kalk KH , Janssen DB , Dijkstra BW |
| Ref : Biochemistry , 32 :9031 , 1993 |
| Abstract : |
| PubMedSearch : Verschueren_1993_Biochemistry_32_9031 |
| PubMedID: 8369276 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Non-covalent binding of the heavy atom compound [Au(CN)2]- at the halide binding site of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 - Verschueren_1993_FEBS.Lett_323_267 |
| Author(s) : Verschueren KH , Franken SM , Rozeboom HJ , Kalk KH , Dijkstra BW |
| Ref : FEBS Letters , 323 :267 , 1993 |
| Abstract : |
| PubMedSearch : Verschueren_1993_FEBS.Lett_323_267 |
| PubMedID: 8500621 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Crystal structure of haloalkane dehalogenase: an enzyme to detoxify halogenated alkanes - Franken_1991_EMBO.J_10_1297 |
| Author(s) : Franken SM , Rozeboom HJ , Kalk KH , Dijkstra BW |
| Ref : EMBO Journal , 10 :1297 , 1991 |
| Abstract : |
| PubMedSearch : Franken_1991_EMBO.J_10_1297 |
| PubMedID: 2026135 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Involvement of a large plasmid in the degradation of 1,2-dichloroethane by Xanthobacter autotrophicus - Tardif_1991_Appl.Environ.Microbiol_57_1853 |
| Author(s) : Tardif G , Greer CW , Labbe D , Lau PC |
| Ref : Applied Environmental Microbiology , 57 :1853 , 1991 |
| Abstract : |
| PubMedSearch : Tardif_1991_Appl.Environ.Microbiol_57_1853 |
| PubMedID: 1872615 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Characterization of the haloacid dehalogenase from Xanthobacter autotrophicus GJ10 and sequencing of the dhlB gene - van der Ploeg_1991_J.Bacteriol_173_7925 |
| Author(s) : van der Ploeg J , van Hall G , Janssen DB |
| Ref : Journal of Bacteriology , 173 :7925 , 1991 |
| Abstract : |
| PubMedSearch : van der Ploeg_1991_J.Bacteriol_173_7925 |
| PubMedID: 1744048 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene - Janssen_1989_J.Bacteriol_171_6791 |
| Author(s) : Janssen DB , Pries F , van der Ploeg J , Kazemier B , Terpstra P , Witholt B |
| Ref : Journal of Bacteriology , 171 :6791 , 1989 |
| Abstract : |
| PubMedSearch : Janssen_1989_J.Bacteriol_171_6791 |
| PubMedID: 2687254 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Crystallization of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 - Rozeboom_1988_J.Mol.Biol_200_611 |
| Author(s) : Rozeboom HJ , Kingma J , Janssen DB , Dijkstra BW |
| Ref : Journal of Molecular Biology , 200 :611 , 1988 |
| Abstract : |
| PubMedSearch : Rozeboom_1988_J.Mol.Biol_200_611 |
| PubMedID: 3398051 |
| Gene_locus related to this paper: xanau-halo1 |
| Title : Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 - Keuning_1985_J.Bacteriol_163_635 |
| Author(s) : Keuning S , Janssen DB , Witholt B |
| Ref : Journal of Bacteriology , 163 :635 , 1985 |
| Abstract : |
| PubMedSearch : Keuning_1985_J.Bacteriol_163_635 |
| PubMedID: 4019411 |
| Gene_locus related to this paper: xanau-halo1 |