Bao X

References (16)

Title : Dipeptidyl peptidase-4-mediated fibronectin processing evokes a pro-fibrotic extracellular matrix - Zeyer_2024_J.Invest.Dermatol__
Author(s) : Zeyer KA , Bornert O , Nelea V , Bao X , Leytens A , Sharoyan S , Sengle G , Antonyan A , Bruckner-Tuderman L , Dengjel J , Reinhardt DP , Nystrom A
Ref : Journal of Investigative Dermatology , : , 2024
Abstract : Fibronectin serves as a platform to guide and facilitate deposition of collagen and fibrillin microfibrils. During development of fibrotic diseases, altered fibronectin deposition in the extracellular matrix (ECM) is generally an early event. Following this, dysregulated organization of fibrillins and fibrillar collagens occurs. Because fibronectin is an essential orchestrator of healthy ECM, perturbation of its ECM-organizational capacity may be involved in development of fibrosis. To investigate this, we employed recessive dystrophic epidermolysis bullosa (RDEB) as a disease model with progressive, severe dermal fibrosis. Fibroblasts from RDEB donors in 2D and 3D cultures displayed dysregulated fibronectin deposition. Our analyses revealed that increase of pro-fibrotic DPP4(+) fibroblasts coincides with altered fibronectin deposition. DPP4 inhibitors normalized deposition of fibronectin and subsequently of fibrillin microfibrils and collagen I. Intriguingly, proteomics, inhibitor and mutagenesis studies disclosed that DPP4 modulates ECM deposition through proteolysis of the fibronectin N-terminus. Our study provides mechanistic insights to the observed pro-fibrotic activities of DPP4 and extends the understanding of fibronectin-guided ECM assembly in health and disease.
ESTHER : Zeyer_2024_J.Invest.Dermatol__
PubMedSearch : Zeyer_2024_J.Invest.Dermatol__
PubMedID: 38570029

Title : Wwl70-induced ABHD6 inhibition attenuates memory deficits and pathological phenotypes in APPswe\/PS1dE9 mice - Xue_2023_Pharmacol.Res__106864
Author(s) : Xue Z , Ye L , Ge J , Lan Z , Zou X , Mao C , Bao X , Yu L , Xu Y , Zhu X
Ref : Pharmacol Res , :106864 , 2023
Abstract : Synaptic dysfunction plays a crucial role in the pathogenesis of Alzheimer's disease (AD). alpha/beta-hydrolase domain-containing 6 (ABHD6) contributes to synaptic dysfunctions, and ABHD6 inhibition has shown potential therapeutic value in neurological disorders. However, the role of ABHD6 in AD has not been fully defined. In this study, we demonstrated that adeno-associated virus (AAV) mediated shRNA targeting ABHD6 in hippocampal neurons attenuated synaptic dysfunction and memory impairment of APPswe/PS1dE9 (APP/PS1) mice, while it didn't affect the amyloid-beta (Abeta) levels and neuroinflammation in the brains. In addition, intraperitoneal injection of wwl70, a specific inhibitor of ABHD6, improved synaptic plasticity and memory function in APP/PS1 mice, which might attribute to the activation of endogenous cannabinoid signaling. Furthermore, wwl70 significantly decreased the Abeta levels and neuroinflammation in the hippocampus of AD mice, and enhanced Abeta phagocytized by microglia. In conclusion, for the first time our data have shown that ABHD6 inhibition might be a promising strategy for AD treatment, and wwl70 is a potential candidate for AD drug development pipeline.
ESTHER : Xue_2023_Pharmacol.Res__106864
PubMedSearch : Xue_2023_Pharmacol.Res__106864
PubMedID: 37480972
Gene_locus related to this paper: human-ABHD6 , mouse-ABHD6

Title : Expression and characterization of a novel lipase from Bacillus licheniformis NCU CS-5 for application in enhancing fatty acids flavor release for low-fat cheeses - Zhao_2022_Food.Chem_368_130868
Author(s) : Zhao J , Ma M , Yan X , Zhang G , Xia J , Zeng G , Tian W , Bao X , Zeng Z , Yu P , Gong D
Ref : Food Chem , 368 :130868 , 2022
Abstract : A novel lipase from Bacillus licheniformis NCU CS-5 was expressed in different Escherichia coli cells. The recombinant enzyme achieved a high activity (161.74 U/mL) with protein concentration of 0.27 mg/mL under optimal conditions at the large-scale expression of 12 h. The recombinant lipase showed optimal activity at 40 degC and pH 10.0, and maintained more than 80% relative activity after 96 h of incubation at pH 9.0-10.0. This typical alkaline lipase was activated under medium temperature conditions (30 and 45 degC for 96 h). The lipase exhibited a degree of adaptability in various organic solvents and metal ions, and showed high specificity towards triglycerides with short and medium chain fatty acids. Among different substrates, the lipase showed the strongest binding affinity towards pNPP (Km = 0.674 mM, Vmax = 950.196 microM/min). In the experiments of its application in enhancing fatty acids flavor release for low-fat cheeses, the lipase was found to hydrolyze cheeses and mainly increase the contents of butyric acid, hexanoic acid, caprylic acid and decanoic acid. The results from NMR and GC provided the possibility of enhancing fatty acids flavor released from low-fat cheeses by the lipolysis method.
ESTHER : Zhao_2022_Food.Chem_368_130868
PubMedSearch : Zhao_2022_Food.Chem_368_130868
PubMedID: 34438173
Gene_locus related to this paper: bacld-q65hr4

Title : Synthesis, in vitro cholinesterase inhibition, molecular docking, DFT and ADME studies of novel 1,3,4-oxadiazole 2-thiol derivatives - Tariq_2022_Chem.Biodivers__
Author(s) : Tariq S , Mutahir S , Khan MA , Mutahir Z , Hussain S , Ashraf M , Bao X , Zhou B , Stark CB , Khan IU
Ref : Chem Biodivers , : , 2022
Abstract : A sequence of 1,3,4-oxadiazole 2-thiol derivatives bearing various alkyl or aryl moieties was designed, synthesized, and characterized by modern spectroscopic methods to yield 17 compounds ( 6a - 6q ) which were screened for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes in search of 'lead' compounds for the treatment of Alzheimer disease (AD). The compounds 6q, 6p, 6k, 6o, and 6l showed inhibitory capability against AChE and BChE, with IC 50 values ranging from 11.730.49 to 27.360.29 microM for AChE and 21.830.39 to 39.430.44 microM for BChE, inhibiting both enzymes within a limited range. The SAR ascertained that the substitution of the aromatic moiety had a profound effect on the AChE and BChE inhibitory potential as compared to the aliphatic substitutions which were supported by the molecular docking studies. In silico ADME studies reinforced the drug-likeness of most of the synthesized molecules. These results were additionally supplemented by the molecular orbital analysis (HOMO-LUMO) and electrostatic potential maps got from DFT calculations. ESP maps expose that on all structures, there are two potential binding sites conquered by the most positive and most negative districts.
ESTHER : Tariq_2022_Chem.Biodivers__
PubMedSearch : Tariq_2022_Chem.Biodivers__
PubMedID: 35767725

Title : Pisa syndrome in dementia with Lewy bodies: A Chinese multicenter study - Su_2022_Parkinsonism.Relat.Disord_103_50
Author(s) : Su Z , Liu S , Chen G , Gan J , Bao X , Zhu H , Wang X , Wu H , Ji Y
Ref : Parkinsonism Relat Disord , 103 :50 , 2022
Abstract : BACKGROUND: Pisa syndrome (PS) is rarely reported in Dementia with Lewy bodies (DLB). The aim of this article is to investigate the prevalence rate of PS and the correlation with clinical features evaluated in patients with DLB. METHODS: A total of 209 DLB patients were consecutively recruited and underwent standardized clinical evaluation in our multicenter study. The associations between PS and clinical factors were evaluated. RESULTS: The prevalence rate of PS in patients with DLB was 15.3%, which was higher in the moderate and severe stages than mild cognitive impairment and mild stages (81.2% vs. 18.8%). Patients with PS had a longer duration of disease (P = 0.020) and parkinsonism (P = 0.003), higher scores of NPI (P = 0.028), ADL (P = 0.002) and UPDRS part III (P < 0.001), lower scores of clock drawing test (P = 0.009), visuospatial/executive abilities (P = 0.018), attention (P = 0.020), language and praxis (P = 0.020), registration (P = 0.012), greater H&Y stage (P < 0.001), and higher proportion of cholinesterase inhibitors used (P = 0.044) than those without PS. Longer disease duration (OR = 1.166, P = 0.023), presence of parkinsonism (OR = 7.971, P = 0.007), moderate and severe dementia (OR = 3.215, P = 0.021) were associated with the presence of PS. Patients had a longer duration of PS (P = 0.014) and lower mean age of onset (P = 0.040) in the group with severe lateral trunk flexion. CONCLUSION: The development of PS may be associated with longer disease duration, the presence of parkinsonism and severe stages of dementia in DLB. Cholinesterase inhibitors may have a correlation with PS. The severity of lateral flexion is related to the duration of PS and mean age of onset.
ESTHER : Su_2022_Parkinsonism.Relat.Disord_103_50
PubMedSearch : Su_2022_Parkinsonism.Relat.Disord_103_50
PubMedID: 36041278

Title : Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice - Bao_2022_J.Mol.Cell.Biol__
Author(s) : Bao X , Ma X , Huang R , Chen J , Xin H , Zhou M , Li L , Tong S , Zhang Q , Shui G , Deng F , Yu L , Li MD , Zhang Z
Ref : J Molecular & Cellular Biology , : , 2022
Abstract : Comparative gene identification-58 (CGI-58), also known as alpha/beta hydrolase domain containing 5 (ABHD5), is the co-activator of adipose triglyceride lipase that hydrolyzes triglycerides stored in the cytosolic lipid droplets. Mutations in CGI-58 gene cause Chanarin-Dorfman syndrome (CDS), an autosomal recessive neutral lipid storage disease with ichthyosis. The liver pathology of CDS manifests as steatosis and steatohepatitis, which currently has no effective treatments. Perilipin-3 (Plin3) is a member of the Perilipin-ADRP-TIP47 (PAT) protein family that is essential for lipid droplet biogenesis. The objective of this study was to test a hypothesis that deletion of a major lipid droplet protein alleviates fatty liver pathogenesis caused by CGI-58 deficiency in hepatocytes. Adult CGI-58-floxed mice were injected with adeno-associated vectors simultaneously expressing the Cre recombinase and microRNA against Plin3 under the control of a hepatocyte-specific promoter, followed by high-fat diet (HFD) feeding for 6 weeks. Liver and blood samples were then collected from these animals for histological and biochemical analysis. Plin3 knockdown in hepatocytes prevented steatosis, steatohepatitis, and necroptosis caused by hepatocyte CGI-58 deficiency. Our work is the first to show that inhibiting Plin3 in hepatocytes is sufficient to mitigate hepatocyte CGI-58 deficiency-induced hepatic steatosis and steatohepatitis in mice.
ESTHER : Bao_2022_J.Mol.Cell.Biol__
PubMedSearch : Bao_2022_J.Mol.Cell.Biol__
PubMedID: 36107452

Title : Discovery of pyrazolones as novel carboxylesterase 2 inhibitors that potently inhibit the adipogenesis in cells - Qian_2021_Bioorg.Med.Chem_40_116187
Author(s) : Qian XK , Zhang J , Song PF , Zhao YS , Ma HY , Jin Q , Wang DD , Guan XQ , Li SY , Bao X , Zou LW
Ref : Bioorganic & Medicinal Chemistry , 40 :116187 , 2021
Abstract : Carboxylesterase 2 (CES2) is one of the most important Phase I drug metabolizing enzymes in the carboxylesterase family. It plays crucial roles in the bioavailability of oral ester prodrugs and the therapeutic effect of some anticancer drugs such as irinotecan (CPT11) and capecitabine. In addition to the well-known roles of CES2 in xenobiotic metabolism, the enzyme also participates in endogenous metabolism and the production of lipids. In this study, we synthesized a series of pyrazolones and assayed their inhibitory effects against CES2 in vitro. Structure-activity relationship analysis of these pyrazolones reveals that the introduction of 4-methylphenyl unit (R(1)), 4-methylbenzyl (R(2)) and cyclohexyl (R(3)) moieties are beneficial for CES2 inhibition. Guided by these SARs results, 1-cyclohexyl-4-(4-methylbenzyl)-3-p-tolyl-1H- pyrazol-5(4H)-one (27) was designed and synthesized. Further investigations demonstrated that the compound 27 exhibited stronger CES2 inhibition activity with a lower IC(50) value (0.13 microM). The inhibition kinetic study demonstrated that compound 27 inhibited the hydrolysis of CES2-fluorescein diacetate (FD) through non-competitive inhibition. In addition, the molecular docking showed that the core of pyrazolone, the cyclohexane moiety, 4-methylbenzyl and 4-methylphenyl groups in compound 27 all played important roles with the amino acid residues of CSE2. Also, compound 27 could inhibit adipocyte adipogenesis induced by mouse preadipocytes. In brief, we designed and synthesized a novel pyrazolone compound with a strong inhibitory ability on CES2 and could inhibit the adipogenesis induced by mouse preadipocytes, which can be served as a promising lead compound for the development of more potent pyrazolone-type CES2 inhibitors, and also used as a potential tool for exploring the biological functions of CES2 in human being.
ESTHER : Qian_2021_Bioorg.Med.Chem_40_116187
PubMedSearch : Qian_2021_Bioorg.Med.Chem_40_116187
PubMedID: 33965840

Title : Discovery of memantyl urea derivatives as potent soluble epoxide hydrolase inhibitors against lipopolysaccharide-induced sepsis - Du_2021_Eur.J.Med.Chem_223_113678
Author(s) : Du F , Sun W , Morisseau C , Hammock BD , Bao X , Liu Q , Wang C , Zhang T , Yang H , Zhou J , Xiao W , Liu Z , Chen G
Ref : Eur Journal of Medicinal Chemistry , 223 :113678 , 2021
Abstract : Sepsis, a systemic inflammatory response, caused by pathogenic factors including microorganisms, has high mortality and limited therapeutic approaches. Herein, a new soluble epoxide hydrolase (sEH) inhibitor series comprising a phenyl ring connected to a memantyl moiety via a urea or amide linkage has been designed. A preferential urea pharmacophore that improved the binding properties of the compounds was identified for those series via biochemical assay in vitro and in vivo studies. Molecular docking displayed that 3,5-dimethyl on the adamantyl group in B401 could make van der Waals interactions with residues at a hydrophobic pocket of sEH active site, which might indirectly explain the subnanomolar level activities of memantyl urea derivatives in vitro better than AR-9281. Among them, compound B401 significantly improved the inhibition potency with human and murine sEH IC(50) values as 0.4 nM and 0.5 nM, respectively. Although the median survival time of C57BL/6 mice in LPS-induced sepsis model was slightly increased, the survival rate did not reach significant efficacy. Based on safety profile, metabolic stability, pharmacokinetic and in vivo efficacy, B401 demonstrated the proof of potential for this class of memantyl urea-based sEH inhibitors as therapeutic agents in sepsis.
ESTHER : Du_2021_Eur.J.Med.Chem_223_113678
PubMedSearch : Du_2021_Eur.J.Med.Chem_223_113678
PubMedID: 34218083

Title : Design, Synthesis, and Structure-Activity Relationship Study of Pyrazolones as Potent Inhibitors against Pancreatic Lipase - Bao_2021_ChemMedChem__
Author(s) : Bao X , Zhang J , Yang Y , Qian XK , Song PF , Zhao YS , Guan XQ , Zou LW , Wang H
Ref : ChemMedChem , : , 2021
Abstract : Pancreatic lipase (PL), a key target for the prevention and treatment of obesity, plays crucial roles in the hydrolysis and absorption of in dietary fat. In this study, a series of pyrazolones were synthesized and their inhibitory effects against PL were assayed using 4-methylumbelliferyl oleate (4-MUO) as optical substrate for PL. Comprehensive structure-activity relationship analysis of these pyrazolones brought us to design and synthesize a novel compound P32 (5-(naphthalen-2-yl)-2-phenyl-4-(thiophen-2-ylmethyl)-2,4-dihydro-3H-pyrazol-3-one) as a potent mixed-competitive inhibitor against PL (IC50 0.30 microM). In addition, P32 displayed some selectivity over other known serine hydrolase. Molecular docking study for P32 demonstrated that the inhibitory activity of P32 towards PL could be attributed to the Pi-Pi interactions of 2-naphthyl unit (R1) and hydrophobic interactions of phenyl moiety (R3) with the active site of PL, respectively. Thus, P32 could serve as promising lead compound for the development of more efficacious and selective pyrazolones-type PL inhibitors for biomedical applications.
ESTHER : Bao_2021_ChemMedChem__
PubMedSearch : Bao_2021_ChemMedChem__
PubMedID: 33527731

Title : Parental transfer of ethylhexyl methoxy cinnamate and induced biochemical responses in zebra fi sh - Zhou_2018_Aquat.Toxicol_206_24
Author(s) : Zhou R , Lu G , Yan Z , Jiang R , Shen J , Bao X
Ref : Aquat Toxicol , 206 :24 , 2018
Abstract : Ethylhexyl methoxy cinnamate (EHMC) is one of the major organic ultraviolet (UV) filter pollutants in the environment. The purpose of this study was to investigate the parental transfer of EHMC and induced biochemical responses in zebra fi sh (Danio rerio). Zebrafish embryos were exposed to EHMC solution (1, 10, and 100 mug/L) for 4 months until sexual maturation. Then male and female parents were paired to lay eggs. F1 generations were divided into 2 categories: with and without continued EHMC exposure. EHMC was detected in both F0 parents and F1 eggs, indicating that EHMC can accumulate in zebrafish and transfer to offspring through reproduction. The hatching rate decreased and malformation rate increased significantly among parents and progeny embryos in the high concentration exposure group. For 40 dpf (days post-fertilisation) F0 generations, estradiol hormone and vitellogenin (Vtg) contents, the expression levels of Vtg1, P450 aromatase (Cyp19a and Cyp19b), 17beta-hydroxysteroid dehydrogenase (Hsd17b1, Hsd17b3), estrogen receptor-alpha and progesterone receptor in all concentration groups decreased significantly, while androgen receptor increased significantly in 10 and 100 mug/L exposure groups compared with the corresponding control group, showing anti-estrogen and androgen effects. For 120 dpf F0 generations, acetylcholinesterase activity was significantly decreased and glutathione and malondialdehyde levels, superoxide dismutase, catalase and glutathione reductase activities were significantly increased in all treatment groups compared with the corresponding control group. In addition, F1 offspring with or without continued exposure to EHMC suffered similar or stronger oxidative stress compared with their parents. DNA breakage and apoptosis also occurred in 120 dpf parental liver cells in all treatment groups as a result of oxidative damage. Results suggested that EHMC have transfer effects between parents and offspring, which may cause negative effects on growth and development of zebrafish and induce biochemical responses in both parents and offspring.
ESTHER : Zhou_2018_Aquat.Toxicol_206_24
PubMedSearch : Zhou_2018_Aquat.Toxicol_206_24
PubMedID: 30419393

Title : Asymmetric Construction of a Multi-Pharmacophore-Containing Dispirotriheterocyclic Scaffold and Identification of a Human Carboxylesterase 1 Inhibitor - Bao_2018_Org.Lett_20_3394
Author(s) : Bao X , Wei S , Qian X , Qu J , Wang B , Zou L , Ge G
Ref : Org Lett , 20 :3394 , 2018
Abstract : A catalytic asymmetric [3 + 2] cyclization of novel 4-isothiocyanato pyrazolones and isatin-derived ketimines was developed, delivering a wide range of intriguing dispirotriheterocyclic products in high yield with excellent diastereoselectivity and enantioselectivity. A chiral sulfoxide derivative of this dispirocyclic product was identified to be a promising hit of the human carboxylesterase 1 inhibitor, and the significant difference of the activity between two enantiomers emphasized the importance of this asymmetric process.
ESTHER : Bao_2018_Org.Lett_20_3394
PubMedSearch : Bao_2018_Org.Lett_20_3394
PubMedID: 29786435

Title : Prognostic value of immunoscore to identify mortality outcomes in adults with HBV-related primary hepatocellular carcinoma - Yao_2017_Medicine.(Baltimore)_96_e6735
Author(s) : Yao Q , Bao X , Xue R , Liu H , Li J , Dong J , Duan Z , Ren M , Zhao J , Song Q , Yu H , Zhu Y , Lu J , Meng Q
Ref : Medicine (Baltimore) , 96 :e6735 , 2017
Abstract : This study aimed to determine if the immunoscore (IS) staging system would be a potential prognostic factor in hepatitis B virus-related hepatocellular carcinoma (HBV-HCC) in China.IS was performed in a consecutive cohort of HBV-HCC patients (n= 92). CD3+, CD8+, and CD45RO+ T cells were quantified by immunohistochemical analyses. The patients were stratified into 5 IS groups: I0, I1, I2, I3, I4 for every 2 cell phenotypes (IS1 (CD8/CD45RO, IS2 (CD3/CD8), and IS3 (CD3/CD45RO), respectively. ImagePro Plus software was used in the calculation of the paraffin-embedded tumor sections.The staining of CD3+, CD8+, and CD45RO+ cells in the HBV-HCC tissue demonstrated that there were higher density and larger area of lymphocytes in the invasive margins (IM) region than in the center (CT). Univariate analysis showed that preoperative TNM staging (P = .01), serum gamma-glutamyl transpeptidase (GGT) level (P = .03), vascular invasion (P = .00), and density of CD3+T (CT) (P = 0.01) were correlated significantly with disease-free survival (DFS); serum alpha-fetoprotein (AFP) level (P = .02), tumor size (P = .00), serum cholinesterase (CHE) (P = .04), and GGT level (P = .01), density of CD3+T(CT) (P = .00), CD8+T(CT)(P = .00), CD45RO+T(CT) (P = .00), and CD45RO+T (IM) (P = .02) were correlated with overall survival (OS). Multivariate analysis showed that TNM staging was not an independent prognostic factor of DFS and OS. Our results showed ISs did not have a significantly correlation with DFS (P = .35, .19, and .07, respectively), but it was correlated significantly with OS (P = .00, .00, and .00, respectively). There were statistical differences among the OS of every ISs subgroup except I0 and I1 by the Cox regressions analysis.The IS staging was closely related to the outcome of patients. It can compensate the TNM tumor classification system in predicting the prognosis of HBV-HCC patients.
ESTHER : Yao_2017_Medicine.(Baltimore)_96_e6735
PubMedSearch : Yao_2017_Medicine.(Baltimore)_96_e6735
PubMedID: 28445292

Title : Lipid Acyl Chain Remodeling in Yeast - Renne_2015_Lipid.Insights_8_33
Author(s) : Renne MF , Bao X , De Smet CH , de Kroon AI
Ref : Lipid Insights , 8 :33 , 2015
Abstract : Membrane lipid homeostasis is maintained by de novo synthesis, intracellular transport, remodeling, and degradation of lipid molecules. Glycerophospholipids, the most abundant structural component of eukaryotic membranes, are subject to acyl chain remodeling, which is defined as the post-synthetic process in which one or both acyl chains are exchanged. Here, we review studies addressing acyl chain remodeling of membrane glycerophospholipids in Saccharomyces cerevisiae, a model organism that has been successfully used to investigate lipid synthesis and its regulation. Experimental evidence for the occurrence of phospholipid acyl chain exchange in cardiolipin, phosphatidylcholine, phosphatidylinositol, and phosphatidylethanolamine is summarized, including methods and tools that have been used for detecting remodeling. Progress in the identification of the enzymes involved is reported, and putative functions of acyl chain remodeling in yeast are discussed.
ESTHER : Renne_2015_Lipid.Insights_8_33
PubMedSearch : Renne_2015_Lipid.Insights_8_33
PubMedID: 26819558

Title : Gated molecular recognition and dynamic discrimination of guests - Rieth_2010_J.Am.Chem.Soc_132_773
Author(s) : Rieth S , Bao X , Wang BY , Hadad CM , Badjic JD
Ref : Journal of the American Chemical Society , 132 :773 , 2010
Abstract : Some highly efficient enzymes, e.g., acetylcholinesterase, use gating as a tool for controlling the rate by which substrates access their active site to direct product formation. Mastering gated molecular encapsulation could therefore be important for manipulating reactivity in artificial environments, albeit quantitative relationships that describe these processes are unknown. In this work, we examined the interdependence between the thermodynamics (DeltaG(o)) and the kinetics (DeltaG(in)(double dagger) and DeltaG(out)(double dagger)) of encapsulation as mediated by gated molecular basket 1. For a series of isosteric guests (2-6, 106-107 A(3)) entering/exiting 1, we found a linear correlation between the host-guest affinities (DeltaG(o)) and the free energies of the activation (DeltaG(in)(double dagger) and DeltaG(out)(double dagger)), which was fit to the following equation: DeltaG(double dagger) = rhoDeltaG(o) + delta. Markedly, the kinetics for the entrapment of smaller guest 7 (93 A(3)) and bigger guest 8 (121 A(3)) did not follow the free energy trends observed for 2-6. Thus, it appears that the kinetics of the gated encapsulation mediated by 1 is a function of the encapsulation's favorability (DeltaG(o)) and the guest's profile. When the size/shape of guests is kept constant, a linear dependence between the encapsulation potential (DeltaG(o)) and the rate of guests' entering/departing basket (DeltaG(in/out)(double dagger)) holds. However, when the potential (DeltaG(o)) is fixed, the basket discriminates guests on the basis of their size/shape via dynamic modulation of the binding site's access.
ESTHER : Rieth_2010_J.Am.Chem.Soc_132_773
PubMedSearch : Rieth_2010_J.Am.Chem.Soc_132_773
PubMedID: 20038142

Title : [A rapid multi-residual analysis for organophosphorus pesticides in the products of animal origin using gas chromatography coupled with accelerated solvent extraction and gel permeation chromatographic purification] - Wu_2008_Se.Pu_26_577
Author(s) : Wu G , Bao X , Wang H , Yu C , Wu H , Ye Q
Ref : Se Pu , 26 :577 , 2008
Abstract : A rapid method has been developed to determine the multi-residues of 36 organophosphorus pesticides in the products of animal origin using capillary gas chromatography with flame photometric detector (GC-FPD (P)). The organophosphorus pesticides were extracted with acetonitrile by accelerated solvent extraction, and cleaned up by auto gel permeation chromatography and primary secondary amine (PSA) packing material. The collected solution was analyzed by the GC-FPD (P) and quantified by internal standard method. The 36 organophosphorus pesticides were separated efficiently from impurity in high sensitivity and reproducibility by GC-FPD (P). The limits of detection (LODs) ranged from 0.0012 mg/kg (phorate) to 0.014 mg/kg (pyraclofos), and the limits of quantitation (LOQs) ranged from 0.004 mg/kg (phorate) to 0.047 mg/kg (pyraclofos). The recoveries ranged from 58.2% to 106.3% in blank samples spiked with 0.05, 0.1 and 0.2 mg/kg of 36 organophosphorus pesticides. The LODs, LOQs and the recoveries of the method all satisfy the requirement of pesticide residue analysis.
ESTHER : Wu_2008_Se.Pu_26_577
PubMedSearch : Wu_2008_Se.Pu_26_577
PubMedID: 19160756

Title : [Protective effect of exogenous glial cell line derived neurotrophic factor on neurons after sciatic nerve injury in rats] - Chen_2000_Sheng.Li.Xue.Bao_52_295
Author(s) : Chen ZY , Cao L , Lu CL , He C , Bao X
Ref : Sheng Li Xue Bao , 52 :295 , 2000
Abstract : The aim of the study was to investigate the effect of exogenous glial cell line derived neurotrophic factor (GDNF) on spinal cord neurons after sciatic nerve axotomy. Upon silicone tubulization of transected sciatic nerve in the adult rat, either 0.9% saline or GDNF solution was injected into the silicone chamber. It was observed by Nissl and enzyme histochemistry staining that exogenous GDNF decreased lesion induced motor neuron death in lateral nucleus of spinal anterior horn and the changes in activity of cholinesterase and acid phosphatase in spinal cord and sensory ganglions. These results suggest that exogenous GDNF is capable of protecting motor neurons from death induced by peripheral nerve injury.
ESTHER : Chen_2000_Sheng.Li.Xue.Bao_52_295
PubMedSearch : Chen_2000_Sheng.Li.Xue.Bao_52_295
PubMedID: 11951110