Pal S

References (11)

Title : Peptide Amphiphilic Supramolecular Nanogel: Competent Host for Notably Efficient Lipase Catalyzed Hydrolysis of Water Insoluble Substrates - Pal_2023_Chembiochem__e202300253
Author(s) : Pal S , Khan AH , Chowdhury M , Das PK
Ref : Chembiochem , :e202300253 , 2023
Abstract : The present work depicts the development of stable nanogels in aqueous medium that were exploited for efficient surface-active lipase catalyzed hydrolysis of water insoluble substrates. Surfactant coated gel nanoparticles (neutral NG1, anionic NG2 and cationic NG3) were prepared from peptide amphiphilic hydrogelator (G1, G2 and G3, respectively) at different hydrophilic and lipophilic balance (HLB). Chromobacterium viscosum (CV) lipase activity towards hydrolysis of water insoluble substrates (p-nitrophyenyl-n-alkanoates (C4-C10)) in presence of nanogels got remarkably improved by ~1.7-8.0 fold in comparison to that in aqueous buffer and other self-aggregates. Increase in hydrophobicity of substrate led to notable improvement in lipase activity in hydrophilic domain (HLB>8.0) of nanogels. Micro-heterogeneous interface of small sized (10-65 nm) nanogel was found to be an appropriate scaffold for immobilizing surface-active lipase to exhibit superior catalytic efficiency. Concurrently, the flexible conformation of lipase immobilized in nanogels was reflected in its secondary structure having highest a-helix content from the circular dichroism spectra.
ESTHER : Pal_2023_Chembiochem__e202300253
PubMedSearch : Pal_2023_Chembiochem__e202300253
PubMedID: 37232377

Title : Cutting Edge: Dysregulated Endocannabinoid-Rheostat for Plasmacytoid Dendritic Cell Activation in a Systemic Lupus Endophenotype - Rahaman_2019_J.Immunol_202_1674
Author(s) : Rahaman O , Bhattacharya R , Liu CSC , Raychaudhuri D , Ghosh AR , Bandopadhyay P , Pal S , Goswami RP , Sircar G , Ghosh P , Ganguly D
Ref : J Immunol , 202 :1674 , 2019
Abstract : Systemic lupus erythematosus (SLE) is a systemic autoimmune disease, characterized by loss of tolerance toward self nuclear Ags. Systemic induction of type I IFNs plays a pivotal role in SLE, a major source of type I IFNs being the plasmacytoid dendritic cells (pDCs). Several genes have been linked with susceptibility to SLE in genome-wide association studies. We aimed at exploring the role of one such gene, alpha/beta-hydrolase domain-containing 6 (ABHD6), in regulation of IFN-alpha induction in SLE patients. We discovered a regulatory role of ABHD6 in human pDCs through modulating the local abundance of its substrate, the endocannabinoid 2-arachidonyl glycerol (2-AG), and elucidated a hitherto unknown cannabinoid receptor 2 (CB2)-mediated regulatory role of 2-AG on IFN-alpha induction by pDCs. We also identified an ABHD6(High) SLE endophenotype wherein reduced local abundance of 2-AG relieves the CB2-mediated steady-state resistive tuning on IFN-alpha induction by pDCs, thereby contributing to SLE pathogenesis.
ESTHER : Rahaman_2019_J.Immunol_202_1674
PubMedSearch : Rahaman_2019_J.Immunol_202_1674
PubMedID: 30728209
Gene_locus related to this paper: human-ABHD6

Title : Unexpected similarities between C9ORF72 and sporadic forms of ALS\/FTD suggest a common disease mechanism - Conlon_2018_Elife_7_
Author(s) : Conlon EG , Fagegaltier D , Agius P , Davis-Porada J , Gregory J , Hubbard I , Kang K , Kim D , Phatnani H , Kwan J , Sareen D , Broach JR , Simmons Z , Arcila-Londono X , Lee EB , Van Deerlin VM , Shneider NA , Fraenkel E , Ostrow LW , Baas F , Zaitlen N , Berry JD , Malaspina A , Fratta P , Cox GA , Thompson LM , Finkbeiner S , Dardiotis E , Miller TM , Chandran S , Pal S , Hornstein E , MacGowan DJ , Heiman-Patterson T , Hammell MG , Patsopoulos NA , Dubnau J , Nath A , Manley JL
Ref : Elife , 7 : , 2018
Abstract : Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) represent two ends of a disease spectrum with shared clinical, genetic and pathological features. These include near ubiquitous pathological inclusions of the RNA-binding protein (RBP) TDP-43, and often the presence of a GGGGCC expansion in the C9ORF72 (C9) gene. Previously, we reported that the sequestration of hnRNP H altered the splicing of target transcripts in C9ALS patients (Conlon et al., 2016). Here, we show that this signature also occurs in half of 50 postmortem sporadic, non-C9 ALS/FTD brains. Furthermore, and equally surprisingly, these 'like-C9' brains also contained correspondingly high amounts of insoluble TDP-43, as well as several other disease-related RBPs, and this correlates with widespread global splicing defects. Finally, we show that the like-C9 sporadic patients, like actual C9ALS patients, were much more likely to have developed FTD. We propose that these unexpected links between C9 and sporadic ALS/FTD define a common mechanism in this disease spectrum.
ESTHER : Conlon_2018_Elife_7_
PubMedSearch : Conlon_2018_Elife_7_
PubMedID: 30003873

Title : Monosodium glutamate impairs the contraction of uterine visceral smooth muscle ex vivo of rat through augmentation of acetylcholine and nitric oxide signaling pathways - Mondal_2018_Reprod.Biol_18_83
Author(s) : Mondal M , Sarkar K , Nath PP , Khatun A , Pal S , Paul G
Ref : Reprod Biol , 18 :83 , 2018
Abstract : The aim of the study was to examine the toxic effects of Monosodium glutamate (MSG), an extensively used food additive, on the contraction of uterine visceral smooth muscle (UVSM) in rat and to elucidate the probable neurocrine mechanism involved in it. MSG produced significant potentiation of the force and inhibition of frequency of uterus recorded ex vivo in chronic MSG exposure and in single dose acute experiments. MSG also produced significant potentiation of force of acetylcholine induced contraction and no alterations in atropine induced contraction of uterus. Further, MSG produced significant increase in force and frequency of contraction of neostigmine incubated uterus. We have found significant potentiation of the post pause force of contraction of uterus when MSG was applied in adrenaline incubated uterus. MSG also produced significant decrease in frequency of contraction of sodium nitroprusside incubated uterus; increase in frequency of N-omega-Nitro-l-Arginine Methyl Ester incubated uterus and no significant changes in frequency of contraction of methylene blue incubated uterus. These results indicate that MSG potentiates the force of contraction of UVSM predominantly by augmenting the activity of cholinergic intrinsic efferents and inhibits the frequency of contraction probably by augmenting the activity of nitrergic efferents. In conclusion, MSG potentiates the force and inhibits the frequency of contraction of UVSM, and the MSG induced effect is probably mediated through the augmentation of acetylcholine and nitric oxide signaling pathways.
ESTHER : Mondal_2018_Reprod.Biol_18_83
PubMedSearch : Mondal_2018_Reprod.Biol_18_83
PubMedID: 29402603

Title : Acute Toxicity and Oxidative Stress Responses in Tadpole of Skittering Frog, Euphlyctis cyanophlyctis (Schneider, 1799) to Sodium Fluoride Exposure - Pal_2018_Bull.Environ.Contam.Toxicol_100_202
Author(s) : Pal S , Samanta P , Kole D , Mukherjee AK , Ghosh AR
Ref : Bulletin of Environmental Contamination & Toxicology , 100 :202 , 2018
Abstract : This study evaluated the acute toxicity and oxidative stress responses to sodium fluoride (NaF) exposure in tadpoles of the skittering frog, Euphlyctis cyanophlyctis (Schneider 1799). The 96 h LC50 value was found to be 647 mg/L. Biochemical tests were conducted at 10%, 20%, 30%, 40%, 50%, 60%, 70% and 80% of the 96 h LC50 dose. Cholinesterase (ChE) activity was unaffected. Lipid peroxidation levels significantly increased (p < 0.05) at lower concentrations, but decreased significantly with increasing NaF concentrations. Glutathione S-transferase (GST) activity also increased significantly with increasing NaF concentrations. Alkaline phosphatase levels steadily decreased with increasing concentrations of NaF. The responses for the biochemical tests were summarized using an integrated biomarker response (IBR) index approach, which indicated that lower NaF exposures caused higher levels of oxidative stress responses overall. These findings suggest that the IBR index approach may be useful for the quantitative monitoring of NaF toxicity in amphibians.
ESTHER : Pal_2018_Bull.Environ.Contam.Toxicol_100_202
PubMedSearch : Pal_2018_Bull.Environ.Contam.Toxicol_100_202
PubMedID: 29294177

Title : A novel esterase subfamily with alpha\/beta-hydrolase fold suggested by structures of two bacterial enzymes homologous to l-homoserine O-acetyl transferases - Tolzer_2016_FEBS.Lett_590_174
Author(s) : Tolzer C , Pal S , Watzlawick H , Altenbuchner J , Niefind K
Ref : FEBS Letters , 590 :174 , 2016
Abstract : MekB from Pseudomonas veronii and CgHle from Corynebacteriumglutamicum belong to the superfamily of alpha/beta-hydrolase fold proteins. Based on sequence comparisons, they are annotated as homoserine transacetylases in popular databases like UNIPROT, PFAM or ESTHER. However, experimentally, MekB and CgHle were shown to be esterases that hydrolyse preferentially acetic acid esters. We describe the x-ray structures of these enzymes solved to high resolution. The overall structures confirm the close relatedness to experimentally validated homoserine acetyl transferases, but simultaneously the structures exclude the ability of MekB and CgHle to bind homoserine and acetyl-CoA. Insofar the MekB and CgHle structures suggest dividing the homoserine transacetylase family into subfamilies, namely genuine acetyl transferases and acetyl esterases with MekB and CgHle as constituting members of the latter.
ESTHER : Tolzer_2016_FEBS.Lett_590_174
PubMedSearch : Tolzer_2016_FEBS.Lett_590_174
PubMedID: 26787467
Gene_locus related to this paper: 9psed-q0mrg5

Title : Effects of chlorpyrifos on the metabolome of the freshwater carp, Cyprinus carpio - Kokushi_2015_Environ.Toxicol_30_253
Author(s) : Kokushi E , Uno S , Pal S , Koyama J
Ref : Environ Toxicol , 30 :253 , 2015
Abstract : This study investigated the effects of waterborne chlorpyrifos with concentrations of 1 and 100 microg/L for L and H-groups, respectively, on metabolome profiles of carp plasma using (1)H-NMR. Principal component analysis suggests that chlorpyrifos exposure firstly affected in L and H-groups on day 2 or 4, and followed a second effect in both exposure groups on day 14. Levels of metabolites related to the energy production in the body, such as glucose, glycerol, valine, leucine, isoleucine, lactate, alanine, 3-D-hydroxybutyrates and acetoacetate, significantly changed by exposures of chlorpyrifos. Those results suggest that energy production was severely affected in carp. The exposure could also be highly elevated ammonia levels especially in H-group due to severe convulsion in muscle caused by the inhibition of acetylcholinesterase activity.
ESTHER : Kokushi_2015_Environ.Toxicol_30_253
PubMedSearch : Kokushi_2015_Environ.Toxicol_30_253
PubMedID: 23997021

Title : Biochemical effects of glyphosate based herbicide, Excel Mera 71 on enzyme activities of acetylcholinesterase (AChE), lipid peroxidation (LPO), catalase (CAT), glutathione-S-transferase (GST) and protein content on teleostean fishes - Samanta_2014_Ecotoxicol.Environ.Saf_107C_120
Author(s) : Samanta P , Pal S , Mukherjee AK , Ghosh AR
Ref : Ecotoxicology & Environmental Safety , 107C :120 , 2014
Abstract : Effects of glyphosate based herbicide, Excel Mera 71 at a dose of 17.20mg/l on enzyme activities of acetylcholinesterase (AChE), lipid peroxidation (LPO), catalase (CAT), glutathione-S-transferase (GST) and protein content were measured in different tissues of two Indian air-breathing teleosts, Anabas testudineus (Bloch) and Heteropneustes fossilis (Bloch) during an exposure period of 30 days under laboratory condition. AChE activity was significantly increased in all the investigated tissues of both fish species and maximum elevation was observed in brain of H. fossilis, while spinal cord of A. testudineus showed minimum increment. Fishes showed significant increase LPO levels in all the tissues; highest was observed in gill of A. testudineus but lowest LPO level was observed in muscle of H. fossilis. CAT was also enhanced in both the fishes, while GST activity in liver diminished substantially and minimum was observed in liver of A. testudineus. Total protein content showed decreased value in all the tissues, maximum reduction was observed in liver and minimum in brain of A. testudineus and H. fossilis respectively. The results indicated that Excel Mera 71 caused serious alterations in the enzyme activities resulting into severe deterioration of fish health; so, AChE, LPO, CAT and GST can be used as suitable indicators of herbicidal toxicity.
ESTHER : Samanta_2014_Ecotoxicol.Environ.Saf_107C_120
PubMedSearch : Samanta_2014_Ecotoxicol.Environ.Saf_107C_120
PubMedID: 24927388

Title : Investigating the mode of action of sulfoxaflor: a fourth-generation neonicotinoid - Cutler_2013_Pest.Manag.Sci_69_607
Author(s) : Cutler P , Slater R , Edmunds AJ , Maienfisch P , Hall RG , Earley FG , Pitterna T , Pal S , Paul VL , Goodchild J , Blacker M , Hagmann L , Crossthwaite AJ
Ref : Pest Manag Sci , 69 :607 , 2013
Abstract : BACKGROUND: The precise mode of action of sulfoxaflor, a new nicotinic acetylcholine receptor-modulating insecticide, is unclear. A detailed understanding of the mode of action, especially in relation to the neonicotinoids, is essential for recommending effective pest management practices.
RESULTS: Radiolabel binding experiments using a tritiated analogue of sulfoxaflor ([(3) H]-methyl-SFX) performed on membranes from Myzus persicae demonstrate that sulfoxaflor interacts specifically with the high-affinity imidacloprid binding site present in a subpopulation of the total nAChR pool. In competition studies, imidacloprid-like neonicotinoids displace [(3) H]-methyl-SFX at pM concentrations. The effects of sulfoxaflor on the exposed aphid nervous system in situ are analogous to those of imidacloprid and nitenpyram, and finally the high-affinity sulfoxaflor binding site is absent in a Myzus persicae strain (clone FRC) possessing a single amino acid point mutation (R81T) in the beta-nAChR, a region critical for neonicotinoid interaction. CONCLUSION: The nicotinic acetylcholine receptor pharmacological profile of sulfoxaflor in aphids is consistent with that of imidacloprid. Additionally, the insecticidal activity of sulfoxaflor and the current commercialised neonicotinoids is affected by the point mutation in FRC Myzus persicae. Therefore, it is suggested that sulfoxalfor be considered a neonicotinoid, and that this be taken into account when recommending insecticide rotation partnering for effective resistance management programmes.
ESTHER : Cutler_2013_Pest.Manag.Sci_69_607
PubMedSearch : Cutler_2013_Pest.Manag.Sci_69_607
PubMedID: 23112103

Title : Crystallization and preliminary crystallographic analysis of cgHle, a homoserine acetyltransferase homologue, from Corynebacterium glutamicum - Tolzer_2009_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_65_34
Author(s) : Tolzer C , Pal S , Watzlawick H , Altenbuchner J , Niefind K
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 65 :34 , 2009
Abstract : CgHle is an enzyme that is encoded by gene cg0961 from Corynebacterium glutamicum. The physiological function of cgHle is so far unclear. Bioinformatic annotations based on sequence homology indicated that cgHle may be an acetyl-CoA:homoserine acetyl transferase and as such may be involved in methionine biosynthesis, but recent evidence has shown that it is an esterase that catalyzes the hydrolysis of acetyl esters. Here, the crystallization of cgHle in two orthorhombic crystal forms, a trigonal crystal form and a monoclinic crystal form is described. The trigonal crystals have a solvent content of 83.7%, which is one of the highest solvent contents ever found for protein crystals. One of the orthorhombic crystals diffracted X-rays to at least 1.2 A resolution.
ESTHER : Tolzer_2009_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_65_34
PubMedSearch : Tolzer_2009_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_65_34
PubMedID: 19153452
Gene_locus related to this paper: corgl-CGL0839

Title : Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes - Seshadri_2004_Proc.Natl.Acad.Sci.U.S.A_101_5646
Author(s) : Seshadri R , Myers GS , Tettelin H , Eisen JA , Heidelberg JF , Dodson RJ , Davidsen TM , DeBoy RT , Fouts DE , Haft DH , Selengut J , Ren Q , Brinkac LM , Madupu R , Kolonay J , Durkin SA , Daugherty SC , Shetty J , Shvartsbeyn A , Gebregeorgis E , Geer K , Tsegaye G , Malek J , Ayodeji B , Shatsman S , McLeod MP , Smajs D , Howell JK , Pal S , Amin A , Vashisth P , McNeill TZ , Xiang Q , Sodergren E , Baca E , Weinstock GM , Norris SJ , Fraser CM , Paulsen IT
Ref : Proc Natl Acad Sci U S A , 101 :5646 , 2004
Abstract : We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis of the T. denticola genome reveals factors mediating coaggregation, cell signaling, stress protection, and other competitive and cooperative measures, consistent with its pathogenic nature and lifestyle within the mixed-species environment of subgingival dental plaque. Comparisons with previously sequenced spirochete genomes revealed specific factors contributing to differences and similarities in spirochete physiology as well as pathogenic potential. The T. denticola genome is considerably larger in size than the genome of the related syphilis-causing spirochete Treponema pallidum. The differences in gene content appear to be attributable to a combination of three phenomena: genome reduction, lineage-specific expansions, and horizontal gene transfer. Genes lost due to reductive evolution appear to be largely involved in metabolism and transport, whereas some of the genes that have arisen due to lineage-specific expansions are implicated in various pathogenic interactions, and genes acquired via horizontal gene transfer are largely phage-related or of unknown function.
ESTHER : Seshadri_2004_Proc.Natl.Acad.Sci.U.S.A_101_5646
PubMedSearch : Seshadri_2004_Proc.Natl.Acad.Sci.U.S.A_101_5646
PubMedID: 15064399
Gene_locus related to this paper: trede-q73j01 , trede-q73kf5 , trede-q73kp3 , trede-q73ks1 , trede-q73nf8 , trede-q73qt5 , trede-q73qv0 , trede-q73ra4 , trede-q73ri8 , trede-Q93EK3 , trede-TDE0521