Qin S

References (14)

Title : The pathogenic mutations of APOA5 in Chinese patients with hyperlipidemic acute pancreatitis - Liu_2024_Lipids.Health.Dis_23_44
Author(s) : Liu Y , Dai S , Qin S , Zhou J , Wang Z , Yin G
Ref : Lipids Health Dis , 23 :44 , 2024
Abstract : BACKGROUND AND AIMS: To study the role of gene mutations in the development of severe hypertriglyceridemia (HTG) in patients with hyperlipidemic acute pancreatitis (HLAP), especially different apolipoprotein A5 (APOA5) mutations. METHODS: Whole-exome sequencing was performed on 163 patients with HLAP and 30 patients with biliary acute pancreatitis (BAP). The pathogenicity of mutations was then assessed by combining clinical information, predictions of bioinformatics programs, information from multiple gene databases, and residue location and conservation. The pathogenic mutations of APOA5 were visualized using the software. RESULTS: 1. Compared with BAP patients, pathogenic mutations of APOA5 were frequent in HLAP patients; among them, the heterozygous mutation of p.G185C was the most common. 2. All six pathogenic mutations of APOA5 identified in this study (p.S35N, p.D167V, p.G185C, p.K188I, p.R223C, and p.H182fs) were positively correlated with severe HTG; they were all in the important domains of apolipoprotein A-V (apoA-V). Residue 223 is strictly conserved in multiple mammals and is located in the lipoprotein lipase (LPL)-binding domain (Pro215-Phe261). When Arg 223 is mutated to Cys 223, the positive charge of this residue is reduced, which is potentially destructive to the binding function of apoA-V to LPL. 3. Four new APOA5 mutations were identified, namely c.563A > T, c.667C > T, c.788G > A, and c.544_545 insGGTGC. CONCLUSIONS: The pathogenic mutations of APOA5 were specific to the patients with HLAP and severe HTG in China, and identifying such mutations had clinical significance in elucidating the etiology and subsequent treatment.
ESTHER : Liu_2024_Lipids.Health.Dis_23_44
PubMedSearch : Liu_2024_Lipids.Health.Dis_23_44
PubMedID: 38331899

Title : Acetylcholine triggered enzymatic cascade reaction based on Fe(7)S(8) nanoflakes catalysis for organophosphorus pesticides visual detection - Zhu_2024_Anal.Chim.Acta_1301_342464
Author(s) : Zhu S , Qin S , Wei C , Cen L , Xiong L , Luo X , Wang Y
Ref : Anal Chim Acta , 1301 :342464 , 2024
Abstract : BACKGROUND: Organophosphorus pesticides (OPs) play important roles in the natural environment, agricultural fields, and biological prevention. The development of OPs detection has gradually become an effective strategy to avoid the dangers of pesticides abuse and solve the severe environmental and health problems in humans. Although conventional assays for OPs analysis such as the bulky instrument required analytical methods have been well-developed, it still remains the limitation of inconvenient, inefficient and lab-dependence analysis in real samples. Hence, there is an urgent demand to develop efficient detection methods for OPs analysis in real scenarios. RESULTS: Here, by virtue of the highly efficient catalytic performance in Fe(7)S(8) nanoflakes (Fe(7)S(8) NFs), we propose an OPs detection method that rationally integrated Fe(7)S(8) NFs into the acetylcholine (ACh) triggered enzymatic cascade reaction (ATECR) for proceeding better detection performances. In this method, OPs serve as the enzyme inhibitors for inhibiting ATECR among ACh, acetylcholinesterase (AChE), and choline oxidase (CHO), then reduce the generation of H(2)O(2) to suppress the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) that catalyzed by Fe(7)S(8) NFs. Benefiting from the integration of Fe(7)S(8) NFs and ATECR, it enables a sensitive detection for OPs (e.g. dimethoate). The proposed method has presented good linear ranges of OPs detection ranging from 0.1 to 10 microg mL(-1). Compared to the other methods, the comparable limits of detection (LOD) of OPs are as low as 0.05 microg mL(-1). SIGNIFICANCE: Furthermore, the proposed method has also achieved a favorable visual detection performance of revealing OPs analysis in real samples. The visual signals of OPs can be transformed into RGB values and gathered by using smartphones, indicating the great potential in simple, sensitive, instrument-free and on-site analysis of pesticide residues in environmental monitoring and biosecurity research.
ESTHER : Zhu_2024_Anal.Chim.Acta_1301_342464
PubMedSearch : Zhu_2024_Anal.Chim.Acta_1301_342464
PubMedID: 38553122

Title : Design, Synthesis, and Biological Evaluation of Novel Tetrahydroacridin Hybrids with Sulfur-Inserted Linkers as Potential Multitarget Agents for Alzheimer's Disease - Wu_2024_Molecules_29_
Author(s) : Wu X , Ze X , Qin S , Zhang B , Li X , Gong Q , Zhang H , Zhu Z , Xu J
Ref : Molecules , 29 : , 2024
Abstract : Alzheimer's disease (AD) is a complex neurodegenerative disease that can lead to the loss of cognitive function. The progression of AD is regulated by multiple signaling pathways and their associated targets. Therefore, multitarget strategies theoretically have greater potential for treating AD. In this work, a series of new hybrids were designed and synthesized by the hybridization of tacrine (4, AChE: IC(50) = 0.223 microM) with pyrimidone compound 5 (GSK-3beta: IC(50) = 3 microM) using the cysteamine or cystamine group as the connector. The biological evaluation results demonstrated that most of the compounds exhibited moderate to good inhibitory activities against acetylcholinesterase (AChE) and glycogen synthase kinase 3beta (GSK-3beta). The optimal compound 18a possessed potent dual AChE/GSK-3beta inhibition (AChE: IC(50) = 0.047 +/- 0.002 microM, GSK-3beta: IC(50) = 0.930 +/- 0.080 microM). Further molecular docking and enzymatic kinetic studies revealed that this compound could occupy both the catalytic anionic site and the peripheral anionic site of AChE. The results also showed a lack of toxicity to SH-SY5Y neuroblastoma cells at concentrations of up to 25 microM. Collectively, this work explored the structure-activity relationships of novel tetrahydroacridin hybrids with sulfur-inserted linkers, providing a reference for the further research and development of new multitarget anti-AD drugs.
ESTHER : Wu_2024_Molecules_29_
PubMedSearch : Wu_2024_Molecules_29_
PubMedID: 38675602

Title : Diketopyrrolopyrrole-based fluorescent probe for visualizing over-expressed carboxylesterase in fever via ratiometric imaging - Zhang_2023_Talanta_266_124971
Author(s) : Zhang B , Qin S , Wang N , Lu X , Jiao J , Zhang J , Zhao W
Ref : Talanta , 266 :124971 , 2023
Abstract : Fever is the result of inflammation and the innate self-defense response of organisms, can cause abnormal changes in the activity of many enzymes in organisms, including the important carboxylesterase (CE). Monitoring the activity changes of CE in vivo during a fever will help to understand heat-related pathological mechanisms. In this paper, we designed diketopyrrolopyrrole-based ratiometric fluorescent probes DPP-FBC-P and DPP-FBO-P containing alkyl chain and diethylene glycol monomethyl ether chain respective for detection of CE. Both probes could realized fast response to CE and displayed good selectivity and high sensitivity. Compared with DPP-FBO-P, DPP-FBC-P had better biocompatibility, larger signal to noise ratio (225-fold vs 125-fold) and lower detection limit (1.6 x 10(-5) U/mL vs 4.2 x 10(-5) U/mL). Moreover, the probe DPP-FBC-P had been successfully applied to image the endogenous CE in HepG2 cells and solid tumors, and also visualized the over expressed CE in fever cells. Most importantly, the changes of CE level in the liver of fever mice model induced by LPS were monitored with the assistance of DPP-FBC-Pvia dual channel ratio imaging for the first time. In addition, fluorescence color signal in solution was captured by smart phone, and the linear relationship between RGB ratio (G/R) and CE concentration was established. This work will provide a potential approach for investigating the physiological and pathological processes of heat related diseases.
ESTHER : Zhang_2023_Talanta_266_124971
PubMedSearch : Zhang_2023_Talanta_266_124971
PubMedID: 37480822

Title : Discovery of Novel Tacrine-Pyrimidone Hybrids as Potent Dual AChE\/GSK-3 Inhibitors for the Treatment of Alzheimer's Disease - Yao_2021_J.Med.Chem__
Author(s) : Yao H , Uras G , Zhang P , Xu S , Yin Y , Liu J , Qin S , Li X , Allen S , Bai R , Gong Q , Zhang H , Zhu Z , Xu J
Ref : Journal of Medicinal Chemistry , : , 2021
Abstract : Based on a multitarget strategy, a series of novel tacrine-pyrimidone hybrids were identified for the potential treatment of Alzheimer's disease (AD). Biological evaluation results demonstrated that these hybrids exhibited significant inhibitory activities toward acetylcholinesterase (AChE) and glycogen synthase kinase 3 (GSK-3). The optimal compound 27g possessed excellent dual AChE/GSK-3 inhibition both in terms of potency and equilibrium (AChE: IC(50) = 51.1 nM; GSK-3beta: IC(50) = 89.3 nM) and displayed significant amelioration on cognitive deficits in scopolamine-induced amnesia mice and efficient reduction against phosphorylation of tau protein on Ser-199 and Ser-396 sites in glyceraldehyde (GA)-stimulated differentiated SH-SY5Y cells. Furthermore, compound 27g exhibited eligible pharmacokinetic properties, good kinase selectivity, and moderate neuroprotection against GA-induced reduction in cell viability and neurite damage in SH-SY5Y-derived neurons. The multifunctional profiles of compound 27g suggest that it deserves further investigation as a promising lead for the prospective treatment of AD.
ESTHER : Yao_2021_J.Med.Chem__
PubMedSearch : Yao_2021_J.Med.Chem__
PubMedID: 34024109

Title : Elucidation of lid open and orientation of lipase activated in interfacial activation by amphiphilic environment - Cheng_2018_Int.J.Biol.Macromol_119_1211
Author(s) : Cheng C , Jiang T , Wu Y , Cui L , Qin S , He B
Ref : Int J Biol Macromol , 119 :1211 , 2018
Abstract : Lipases have wide applications using as biocatalyst in numerous biotechnological and bioengineering fields, especially function at hydrophobic or amphiphilic interface. Previously, the lipase from Burkholderia ambifaria YCJ01 was significantly activated when immobilized on the amphiphilic environment. In this work, insights into the functional effect of amphiphilic surface on lipase activation are presented by molecular dynamic simulations. The notable open of "lid" (alpha5 region) and the displacement of "flap" region (alpha8 region) of the lipase are closely related with the activation mechanism of lipase, which makes the active site accessible. Strikingly, the hydrophobic analysis showed that most of the hydrophobic surface residues of lipase, as an interfacial enzyme, located at the "lid" and "flap" regions make the entry to active site naturally orient to the oil-water interface to achieve enzyme activation. Additionally, the analysis of Rg and hydrogen bonding interaction suggested that the amphiphilic environment benefits to the exposure of hydrophobic regions, especially the "lid" and "flap" regions, and the maintenance of the nonpolar environment of the active site. Observations from this work not only complement the activation mechanism of lipase induced by the amphiphilic environment, but also provide a reference for the engineering of immobilized media for interfacial enzyme.
ESTHER : Cheng_2018_Int.J.Biol.Macromol_119_1211
PubMedSearch : Cheng_2018_Int.J.Biol.Macromol_119_1211
PubMedID: 30071229
Gene_locus related to this paper: 9burk-i3rta9

Title : Anti-phytopathogen, multi-target acetylcholinesterase inhibitory and antioxidant activities of metabolites from endophytic Chaetomium globosum - Li_2016_Nat.Prod.Res__1
Author(s) : Li W , Yang X , Yang Y , Duang R , Chen G , Li X , Li Q , Qin S , Li S , Zhao L , Ding Z
Ref : Nat Prod Res , :1 , 2016
Abstract : Fourteen metabolites with various structure types were isolated from endophytic Chaetomium globosum. Five compounds were separated from genus Chaetomium for the first time. Some compounds exhibited remarkable inhibition against phytopathogenic fungi causing root rot of Panax notoginseng. Compounds 1-5 had significant DPPH-free radical-scavenging activity. Compounds 3 and 5 indicated significant inhibitions against the acetylcholinesterase (AChE). From preliminary structure-activity relationship, it was found that the oxygenic five-membered ring of 3 and 5 was crucial in the anti-AChE activity. These structures provide new templates for the potential treatment and management of plant diseases and Alzheimer disease.
ESTHER : Li_2016_Nat.Prod.Res__1
PubMedSearch : Li_2016_Nat.Prod.Res__1
PubMedID: 26744178

Title : Draft genome sequence of marine Streptomyces sp. strain W007, which produces angucyclinone antibiotics with a benz[a]anthracene skeleton - Qin_2012_J.Bacteriol_194_1628
Author(s) : Qin S , Zhang H , Li F , Zhu B , Zheng H
Ref : Journal of Bacteriology , 194 :1628 , 2012
Abstract : A series of angucyclinone antibiotics have been isolated from marine Streptomyces sp. strain W007 and identified. Here, a draft genome sequence of Streptomyces sp. W007 is presented. The genome contains an intact biosynthetic gene cluster for angucyclinone antibiotics, which provides insight into the combinatorial biosynthesis of angucyclinone antibiotics produced by marine streptomycetes.
ESTHER : Qin_2012_J.Bacteriol_194_1628
PubMedSearch : Qin_2012_J.Bacteriol_194_1628
PubMedID: 22374958
Gene_locus related to this paper: 9actn-h0b8d4 , 9acto-h0b5v2 , 9acto-h0bkj3 , 9acto-h0bln7 , 9acto-h0blv8 , 9acto-h0brh7 , strgg-b1vzw6 , 9acto-h0b9i4 , 9acto-h0bn07 , 9actn-h0bay1

Title : Draft genome sequence of the marine bacterium Streptomyces griseoaurantiacus M045, which produces novel manumycin-type antibiotics with a pABA core component - Li_2011_J.Bacteriol_193_3417
Author(s) : Li F , Jiang P , Zheng H , Wang S , Zhao G , Qin S , Liu Z
Ref : Journal of Bacteriology , 193 :3417 , 2011
Abstract : Streptomyces griseoaurantiacus M045, isolated from marine sediment, produces manumycin and chinikomycin antibiotics. Here we present a high-quality draft genome sequence of S. griseoaurantiacus M045, the first marine Streptomyces species to be sequenced and annotated. The genome encodes several gene clusters for biosynthesis of secondary metabolites and has provided insight into genomic islands linking secondary metabolism to functional adaptation in marine S. griseoaurantiacus M045.
ESTHER : Li_2011_J.Bacteriol_193_3417
PubMedSearch : Li_2011_J.Bacteriol_193_3417
PubMedID: 21551298
Gene_locus related to this paper: 9acto-f3ngb7 , 9acto-f3nim7 , 9acto-f3ntg3 , 9acto-f3nmw3 , 9actn-f3nh76 , 9actn-f3nh94

Title : Genome sequences for five strains of the emerging pathogen Haemophilus haemolyticus - Jordan_2011_J.Bacteriol_193_5879
Author(s) : Jordan IK , Conley AB , Antonov IV , Arthur RA , Cook ED , Cooper GP , Jones BL , Knipe KM , Lee KJ , Liu X , Mitchell GJ , Pande PR , Petit RA , Qin S , Rajan VN , Sarda S , Sebastian A , Tang S , Thapliyal R , Varghese NJ , Ye T , Katz LS , Wang X , Rowe L , Frace M , Mayer LW
Ref : Journal of Bacteriology , 193 :5879 , 2011
Abstract : We report the first whole-genome sequences for five strains, two carried and three pathogenic, of the emerging pathogen Haemophilus haemolyticus. Preliminary analyses indicate that these genome sequences encode markers that distinguish H. haemolyticus from its closest Haemophilus relatives and provide clues to the identity of its virulence factors.
ESTHER : Jordan_2011_J.Bacteriol_193_5879
PubMedSearch : Jordan_2011_J.Bacteriol_193_5879
PubMedID: 21952546
Gene_locus related to this paper: haein-yfbb

Title : Implication of S-adenosylhomocysteine hydrolase in inhibition of TNF-alpha- and IL-1beta-induced expression of inflammatory mediators by AICAR in RPE cells - Qin_2008_Invest.Ophthalmol.Vis.Sci_49_1274
Author(s) : Qin S , Ni M , De Vries GW
Ref : Invest Ophthalmol Vis Sci , 49 :1274 , 2008
Abstract : PURPOSE: AMP-activated protein kinase (AMPK) has been suggested to be a novel signaling pathway in regulating inflammation. The role of AMPK in retinal pigment epithelial cell inflammatory response is addressed using AMPK activator 5-aminoimidazole-4-carboxamide riboside (AICAR).
METHODS: Protein expression and activation of signaling molecules were detected by immunoblotting. Cytokines were determined by ELISA kits. AMPKalpha expression was knockdown by siRNAs.
RESULTS: AICAR inhibited tumor necrosis factor (TNF)-alpha- or interleukin (IL)-1beta-induced production of IL-6, IL-8, and monocyte chemotactic protein (MCP)-1 and of intercellular adhesion molecule (ICAM)-1 expression in human RPE cells. The inhibitory effect on cytokine production and ICAM-1 expression persisted in the RPE cells in which AMPK was knocked down by AMPK siRNA. Moreover, an adenosine kinase inhibitor 5'-iodotubercidin, which effectively abolished AMPK activation caused by AICAR, did not reverse the anti-inflammatory effect of AICAR. In comparison, anti-inflammatory effects of AICAR were mimicked by adenosine but not inosine, the metabolites of AICAR. Finally, with the exception of TNF-alpha-induced IL-6 production, adenosine dialdehyde, an inhibitor of S-adenosylhomocysteine hydrolase, was found to block cytokine production and ICAM-1 expression.
CONCLUSIONS: Regardless of the ability of AICAR to activate AMPK, the inhibitory effects of AICAR on cytokine production and ICAM-1 expression were not associated with AMPK. The mechanism of AICAR inhibition may be attributed to the interference of adenosylmethionine-dependent methylation.
ESTHER : Qin_2008_Invest.Ophthalmol.Vis.Sci_49_1274
PubMedSearch : Qin_2008_Invest.Ophthalmol.Vis.Sci_49_1274
PubMedID: 18326758

Title : Analysis of the DNA sequence and duplication history of human chromosome 15 - Zody_2006_Nature_440_671
Author(s) : Zody MC , Garber M , Sharpe T , Young SK , Rowen L , O'Neill K , Whittaker CA , Kamal M , Chang JL , Cuomo CA , Dewar K , Fitzgerald MG , Kodira CD , Madan A , Qin S , Yang X , Abbasi N , Abouelleil A , Arachchi HM , Baradarani L , Birditt B , Bloom S , Bloom T , Borowsky ML , Burke J , Butler J , Cook A , DeArellano K , Decaprio D , Dorris L, 3rd , Dors M , Eichler EE , Engels R , Fahey J , Fleetwood P , Friedman C , Gearin G , Hall JL , Hensley G , Johnson E , Jones C , Kamat A , Kaur A , Locke DP , Munson G , Jaffe DB , Lui A , Macdonald P , Mauceli E , Naylor JW , Nesbitt R , Nicol R , O'Leary SB , Ratcliffe A , Rounsley S , She X , Sneddon KM , Stewart S , Sougnez C , Stone SM , Topham K , Vincent D , Wang S , Zimmer AR , Birren BW , Hood L , Lander ES , Nusbaum C
Ref : Nature , 440 :671 , 2006
Abstract : Here we present a finished sequence of human chromosome 15, together with a high-quality gene catalogue. As chromosome 15 is one of seven human chromosomes with a high rate of segmental duplication, we have carried out a detailed analysis of the duplication structure of the chromosome. Segmental duplications in chromosome 15 are largely clustered in two regions, on proximal and distal 15q; the proximal region is notable because recombination among the segmental duplications can result in deletions causing Prader-Willi and Angelman syndromes. Sequence analysis shows that the proximal and distal regions of 15q share extensive ancient similarity. Using a simple approach, we have been able to reconstruct many of the events by which the current duplication structure arose. We find that most of the intrachromosomal duplications seem to share a common ancestry. Finally, we demonstrate that some remaining gaps in the genome sequence are probably due to structural polymorphisms between haplotypes; this may explain a significant fraction of the gaps remaining in the human genome.
ESTHER : Zody_2006_Nature_440_671
PubMedSearch : Zody_2006_Nature_440_671
PubMedID: 16572171
Gene_locus related to this paper: human-DPP8 , human-LIPC , human-SPG21

Title : Degradation and adsorption of fosthiazate in soil - Qin_2004_J.Agric.Food.Chem_52_6239
Author(s) : Qin S , Gan J , Liu W , Becker JO
Ref : Journal of Agricultural and Food Chemistry , 52 :6239 , 2004
Abstract : Adsorption and degradation behavior of a pesticide in soil has a strong effect on its environmental fate as well as efficacy for pest control. Fosthiazate is an organophosphate compound that is currently under development as a nonfumigant nematicide. In this study, we evaluated adsorption and degradation kinetics of fosthiazate in three U.S. soils with different properties. Adsorption of fosthiazate in mineral soil was negligibly weak but appeared to increase with soil organic matter (OM) content. The half-life (T(1/2)) of fosthiazate ranged from 0.5 to 1.5 months in nonsterile soils but was prolonged to 1-3 months after sterilization. Degradation of fosthiazate in soil appeared to be caused by both chemical and microbial transformations. The persistence of fosthiazate generally decreased with increasing soil pH, but increased with increasing soil OM and clay contents. This results suggest that fosthiazate may have an enhanced leaching potential in acidic soils with low OM content, and its efficacy in high pH soils may not last as long as in neutral soils because of faster degradation.
ESTHER : Qin_2004_J.Agric.Food.Chem_52_6239
PubMedSearch : Qin_2004_J.Agric.Food.Chem_52_6239
PubMedID: 15453693

Title : Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse - Xie_2003_Genome.Res_13_2621
Author(s) : Xie T , Rowen L , Aguado B , Ahearn ME , Madan A , Qin S , Campbell RD , Hood L
Ref : Genome Res , 13 :2621 , 2003
Abstract : In mammals, the Major Histocompatibility Complex class I and II gene clusters are separated by an approximately 700-kb stretch of sequence called the MHC class III region, which has been associated with susceptibility to numerous diseases. To facilitate understanding of this medically important and architecturally interesting portion of the genome, we have sequenced and analyzed both the human and mouse class III regions. The cross-species comparison has facilitated the identification of 60 genes in human and 61 in mouse, including a potential RNA gene for which the introns are more conserved across species than the exons. Delineation of global organization, gene structure, alternative splice forms, protein similarities, and potential cis-regulatory elements leads to several conclusions: (1) The human MHC class III region is the most gene-dense region of the human genome: >14% of the sequence is coding, approximately 72% of the region is transcribed, and there is an average of 8.5 genes per 100 kb. (2) Gene sizes, number of exons, and intergenic distances are for the most part similar in both species, implying that interspersed repeats have had little impact in disrupting the tight organization of this densely packed set of genes. (3) The region contains a heterogeneous mixture of genes, only a few of which have a clearly defined and proven function. Although many of the genes are of ancient origin, some appear to exist only in mammals and fish, implying they might be specific to vertebrates. (4) Conserved noncoding sequences are found primarily in or near the 5'-UTR or the first intron of genes, and seldom in the intergenic regions. Many of these conserved blocks are likely to be cis-regulatory elements.
ESTHER : Xie_2003_Genome.Res_13_2621
PubMedSearch : Xie_2003_Genome.Res_13_2621
PubMedID: 14656967
Gene_locus related to this paper: mouse-PPT2