Yamaguchi K

References (10)

Title : Shark genomes provide insights into elasmobranch evolution and the origin of vertebrates - Hara_2018_Nat.Ecol.Evol_2_1761
Author(s) : Hara Y , Yamaguchi K , Onimaru K , Kadota M , Koyanagi M , Keeley SD , Tatsumi K , Tanaka K , Motone F , Kageyama Y , Nozu R , Adachi N , Nishimura O , Nakagawa R , Tanegashima C , Kiyatake I , Matsumoto R , Murakumo K , Nishida K , Terakita A , Kuratani S , Sato K , Hyodo S , Kuraku S
Ref : Nat Ecol Evol , 2 :1761 , 2018
Abstract : Modern cartilaginous fishes are divided into elasmobranchs (sharks, rays and skates) and chimaeras, and the lack of established whole-genome sequences for the former has prevented our understanding of early vertebrate evolution and the unique phenotypes of elasmobranchs. Here we present de novo whole-genome assemblies of brownbanded bamboo shark and cloudy catshark and an improved assembly of the whale shark genome. These relatively large genomes (3.8-6.7 Gbp) contain sparse distributions of coding genes and regulatory elements and exhibit reduced molecular evolutionary rates. Our thorough genome annotation revealed Hox C genes previously hypothesized to have been lost, as well as distinct gene repertories of opsins and olfactory receptors that would be associated with adaptation to unique underwater niches. We also show the early establishment of the genetic machinery governing mammalian homoeostasis and reproduction at the jawed vertebrate ancestor. This study, supported by genomic, transcriptomic and epigenomic resources, provides a foundation for the comprehensive, molecular exploration of phenotypes unique to sharks and insights into the evolutionary origins of vertebrates.
ESTHER : Hara_2018_Nat.Ecol.Evol_2_1761
PubMedSearch : Hara_2018_Nat.Ecol.Evol_2_1761
PubMedID: 30297745
Gene_locus related to this paper: scyto-a0a401nql2 , chipu-a0a401shd8 , chipu-a0a401rzt4 , scyto-a0a401q2n8 , scyto-a0a401nqq7 , chipu-a0a401s2p9 , scyto-a0a401q2m6 , chipu-a0a401rz56

Title : Suppression of ABHD2, identified through a functional genomics screen, causes anoikis resistance, chemoresistance and poor prognosis in ovarian cancer - Yamanoi_2016_Oncotarget_7_47620
Author(s) : Yamanoi K , Matsumura N , Murphy SK , Baba T , Abiko K , Hamanishi J , Yamaguchi K , Koshiyama M , Konishi I , Mandai M
Ref : Oncotarget , 7 :47620 , 2016
Abstract : Anoikis resistance is a hallmark of cancer, and relates to malignant phenotypes, including chemoresistance, cancer stem like phenotypes and dissemination. The aim of this study was to identify key factors contributing to anoikis resistance in ovarian cancer using a functional genomics screen. A library of 81 000 shRNAs targeting 15 000 genes was transduced into OVCA420 cells, followed by incubation in soft agar and colony selection. We found shRNAs directed to ABHD2, ELAC2 and CYB5R3 caused reproducible anoikis resistance. These three genes are deleted in many serous ovarian cancers according to The Cancer Genome Atlas data. Suppression of ABHD2 in OVCA420 cells increased phosphorylated p38 and ERK, platinum resistance, and side population cells (p<0.01, respectively). Conversely, overexpression of ABHD2 decreased resistance to anoikis (p<0.05) and the amount of phosphorylated p38 and ERK in OVCA420 and SKOV3 cells. In clinical serous ovarian cancer specimens, low expression of ABHD2 was associated with platinum resistance and poor prognosis (p<0.05, respectively). In conclusion, we found three novel genes relevant to anoikis resistance in ovarian cancer using a functional genomics screen. Suppression of ABHD2 may promote a malignant phenotype and poor prognosis for women with serous ovarian cancer.
ESTHER : Yamanoi_2016_Oncotarget_7_47620
PubMedSearch : Yamanoi_2016_Oncotarget_7_47620
PubMedID: 27323405

Title : Complete Genome Sequence of Burkholderia sp. Strain RPE64, Bacterial Symbiont of the Bean Bug Riptortus pedestris - Shibata_2013_Genome.Announc_1_e00441
Author(s) : Shibata TF , Maeda T , Nikoh N , Yamaguchi K , Oshima K , Hattori M , Nishiyama T , Hasebe M , Fukatsu T , Kikuchi Y , Shigenobu S
Ref : Genome Announc , 1 :e00441 , 2013
Abstract : We isolated Burkholderia symbiont strain RPE64 from the bean bug Riptortus pedestris. Analysis of the complete 6.96-Mb genome, which consists of three chromosomes and two plasmids, will facilitate further understanding of insect-microbe symbiosis and the development of pest-control technologies.
ESTHER : Shibata_2013_Genome.Announc_1_e00441
PubMedSearch : Shibata_2013_Genome.Announc_1_e00441
PubMedID: 23833137
Gene_locus related to this paper: 9burk-r4wy41 , 9burk-a0a069nn99 , 9burk-r4wtx8 , 9burk-r4wsg0 , 9burk-r4x084 , 9burk-r4wsp2

Title : The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants - Rensing_2008_Science_319_64
Author(s) : Rensing SA , Lang D , Zimmer AD , Terry A , Salamov A , Shapiro H , Nishiyama T , Perroud PF , Lindquist EA , Kamisugi Y , Tanahashi T , Sakakibara K , Fujita T , Oishi K , Shin IT , Kuroki Y , Toyoda A , Suzuki Y , Hashimoto S , Yamaguchi K , Sugano S , Kohara Y , Fujiyama A , Anterola A , Aoki S , Ashton N , Barbazuk WB , Barker E , Bennetzen JL , Blankenship R , Cho SH , Dutcher SK , Estelle M , Fawcett JA , Gundlach H , Hanada K , Heyl A , Hicks KA , Hughes J , Lohr M , Mayer K , Melkozernov A , Murata T , Nelson DR , Pils B , Prigge M , Reiss B , Renner T , Rombauts S , Rushton PJ , Sanderfoot A , Schween G , Shiu SH , Stueber K , Theodoulou FL , Tu H , Van de Peer Y , Verrier PJ , Waters E , Wood A , Yang L , Cove D , Cuming AC , Hasebe M , Lucas S , Mishler BD , Reski R , Grigoriev IV , Quatrano RS , Boore JL
Ref : Science , 319 :64 , 2008
Abstract : We report the draft genome sequence of the model moss Physcomitrella patens and compare its features with those of flowering plants, from which it is separated by more than 400 million years, and unicellular aquatic algae. This comparison reveals genomic changes concomitant with the evolutionary movement to land, including a general increase in gene family complexity; loss of genes associated with aquatic environments (e.g., flagellar arms); acquisition of genes for tolerating terrestrial stresses (e.g., variation in temperature and water availability); and the development of the auxin and abscisic acid signaling pathways for coordinating multicellular growth and dehydration response. The Physcomitrella genome provides a resource for phylogenetic inferences about gene function and for experimental analysis of plant processes through this plant's unique facility for reverse genetics.
ESTHER : Rensing_2008_Science_319_64
PubMedSearch : Rensing_2008_Science_319_64
PubMedID: 18079367
Gene_locus related to this paper: phypa-a9rbi6 , phypa-a9rfh1 , phypa-a9rg19 , phypa-a9rgt9 , phypa-a9rhz9 , phypa-a9rkj1 , phypa-a9rns2 , phypa-a9rp52 , phypa-a9rq03 , phypa-a9ry17 , phypa-a9ry72 , phypa-a9s5n8 , phypa-a9s6w1 , phypa-a9s8c7 , phypa-a9s299 , phypa-a9san7 , phypa-a9sc75 , phypa-a9se75 , phypa-a9sg07 , phypa-a9skf7 , phypa-a9skr1 , phypa-a9skw1 , phypa-a9sl58 , phypa-a9slp7 , phypa-a9smq5 , phypa-a9sp13 , phypa-a9ssb0 , phypa-a9sse1 , phypa-a9ssf6 , phypa-a9st85 , phypa-a9sx74 , phypa-a9sy58 , phypa-a9syy4 , phypa-a9t0n4 , phypa-a9t0p4 , phypa-a9t1j2 , phypa-a9t5h1 , phypa-a9t7g6 , phypa-a9t8u8 , phypa-a9t9c9 , phypa-a9t9d9 , phypa-a0a7i4d2t7 , phypa-a9t498 , phypa-a9tbu4 , phypa-a9tc36 , phypa-a9tds0 , phypa-a9te64 , phypa-a9tfw2 , phypa-a9tin6 , phypa-a9tja4 , phypa-a9tmp3 , phypa-a9tmr4 , phypa-a9tql4 , phypa-a9tr83 , phypa-a9tsl1 , phypa-a9tsv6 , phypa-a9tu05 , phypa-a9tw81 , phypa-a9tyr8 , phypa-a9u0c9 , phypa-a9u0k3 , phypa-a9u0p4 , phypa-a9u2u7 , phypa-a9u3s0 , phypa-a9tfm7 , phypa-a9tfp6 , phypa-a9syg9 , phypa-a9tzk2 , phypa-a9tvg4 , phypa-a9t1y4 , phypa-a9tqt6 , phypa-a9st18 , phypa-a9tix9 , phypa-a0a2k1kfe3 , phypa-a9sqk3 , phypa-a0a2k1ie71 , phypa-a0a2k1kg29 , phypa-a0a2k1iji3

Title : The Rice Annotation Project Database (RAP-DB): 2008 update - Tanaka_2008_Nucleic.Acids.Res_36_D1028
Author(s) : Tanaka T , Antonio BA , Kikuchi S , Matsumoto T , Nagamura Y , Numa H , Sakai H , Wu J , Itoh T , Sasaki T , Aono R , Fujii Y , Habara T , Harada E , Kanno M , Kawahara Y , Kawashima H , Kubooka H , Matsuya A , Nakaoka H , Saichi N , Sanbonmatsu R , Sato Y , Shinso Y , Suzuki M , Takeda J , Tanino M , Todokoro F , Yamaguchi K , Yamamoto N , Yamasaki C , Imanishi T , Okido T , Tada M , Ikeo K , Tateno Y , Gojobori T , Lin YC , Wei FJ , Hsing YI , Zhao Q , Han B , Kramer MR , McCombie RW , Lonsdale D , O'Donovan CC , Whitfield EJ , Apweiler R , Koyanagi KO , Khurana JP , Raghuvanshi S , Singh NK , Tyagi AK , Haberer G , Fujisawa M , Hosokawa S , Ito Y , Ikawa H , Shibata M , Yamamoto M , Bruskiewich RM , Hoen DR , Bureau TE , Namiki N , Ohyanagi H , Sakai Y , Nobushima S , Sakata K , Barrero RA , Souvorov A , Smith-White B , Tatusova T , An S , An G , S OO , Fuks G , Messing J , Christie KR , Lieberherr D , Kim H , Zuccolo A , Wing RA , Nobuta K , Green PJ , Lu C , Meyers BC , Chaparro C , Piegu B , Panaud O , Echeverria M
Ref : Nucleic Acids Research , 36 :D1028 , 2008
Abstract : The Rice Annotation Project Database (RAP-DB) was created to provide the genome sequence assembly of the International Rice Genome Sequencing Project (IRGSP), manually curated annotation of the sequence, and other genomics information that could be useful for comprehensive understanding of the rice biology. Since the last publication of the RAP-DB, the IRGSP genome has been revised and reassembled. In addition, a large number of rice-expressed sequence tags have been released, and functional genomics resources have been produced worldwide. Thus, we have thoroughly updated our genome annotation by manual curation of all the functional descriptions of rice genes. The latest version of the RAP-DB contains a variety of annotation data as follows: clone positions, structures and functions of 31 439 genes validated by cDNAs, RNA genes detected by massively parallel signature sequencing (MPSS) technology and sequence similarity, flanking sequences of mutant lines, transposable elements, etc. Other annotation data such as Gnomon can be displayed along with those of RAP for comparison. We have also developed a new keyword search system to allow the user to access useful information. The RAP-DB is available at: http://rapdb.dna.affrc.go.jp/ and http://rapdb.lab.nig.ac.jp/.
ESTHER : Tanaka_2008_Nucleic.Acids.Res_36_D1028
PubMedSearch : Tanaka_2008_Nucleic.Acids.Res_36_D1028
PubMedID: 18089549
Gene_locus related to this paper: orysa-Q9FW17 , orysa-Q0JK71 , orysa-B9EWJ8 , orysa-Q5N7L1 , orysa-pir7a , orysa-q2qyj1 , orysj-q6yse8 , orysa-q6yzk1 , orysa-Q8S0U8 , orysa-q33aq0 , orysa-Q0J0A4 , orysi-a2z179 , orysi-a2zef2 , orysi-b8a7e6 , orysi-b8a7e7 , orysi-b8bfe5 , orysi-b8bhp9 , orysj-b9fi05 , orysj-b9fkb0 , orysj-cgep , orysj-q0djj0 , orysj-q0dud7 , orysj-q0jaf0 , orysj-q0jga1 , orysj-q5jl22 , orysj-q5jlw7 , orysj-q6h7q9 , orysj-q6yvk6 , orysj-q7f8x1 , orysj-q7xcx3 , orysj-q9fwm6 , orysj-q10j20 , orysj-q10ss2 , orysj-q69uw6 , orysj-q94d71 , orysj-q0iq98 , orysj-b9gbs4 , orysj-b9gbs1 , orysj-pla4 , orysj-pla1

Title : Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana - Itoh_2007_Genome.Res_17_175
Author(s) : Itoh T , Tanaka T , Barrero RA , Yamasaki C , Fujii Y , Hilton PB , Antonio BA , Aono H , Apweiler R , Bruskiewich R , Bureau T , Burr F , Costa de Oliveira A , Fuks G , Habara T , Haberer G , Han B , Harada E , Hiraki AT , Hirochika H , Hoen D , Hokari H , Hosokawa S , Hsing YI , Ikawa H , Ikeo K , Imanishi T , Ito Y , Jaiswal P , Kanno M , Kawahara Y , Kawamura T , Kawashima H , Khurana JP , Kikuchi S , Komatsu S , Koyanagi KO , Kubooka H , Lieberherr D , Lin YC , Lonsdale D , Matsumoto T , Matsuya A , McCombie WR , Messing J , Miyao A , Mulder N , Nagamura Y , Nam J , Namiki N , Numa H , Nurimoto S , O'Donovan C , Ohyanagi H , Okido T , Oota S , Osato N , Palmer LE , Quetier F , Raghuvanshi S , Saichi N , Sakai H , Sakai Y , Sakata K , Sakurai T , Sato F , Sato Y , Schoof H , Seki M , Shibata M , Shimizu Y , Shinozaki K , Shinso Y , Singh NK , Smith-White B , Takeda J , Tanino M , Tatusova T , Thongjuea S , Todokoro F , Tsugane M , Tyagi AK , Vanavichit A , Wang A , Wing RA , Yamaguchi K , Yamamoto M , Yamamoto N , Yu Y , Zhang H , Zhao Q , Higo K , Burr B , Gojobori T , Sasaki T
Ref : Genome Res , 17 :175 , 2007
Abstract : We present here the annotation of the complete genome of rice Oryza sativa L. ssp. japonica cultivar Nipponbare. All functional annotations for proteins and non-protein-coding RNA (npRNA) candidates were manually curated. Functions were identified or inferred in 19,969 (70%) of the proteins, and 131 possible npRNAs (including 58 antisense transcripts) were found. Almost 5000 annotated protein-coding genes were found to be disrupted in insertional mutant lines, which will accelerate future experimental validation of the annotations. The rice loci were determined by using cDNA sequences obtained from rice and other representative cereals. Our conservative estimate based on these loci and an extrapolation suggested that the gene number of rice is approximately 32,000, which is smaller than previous estimates. We conducted comparative analyses between rice and Arabidopsis thaliana and found that both genomes possessed several lineage-specific genes, which might account for the observed differences between these species, while they had similar sets of predicted functional domains among the protein sequences. A system to control translational efficiency seems to be conserved across large evolutionary distances. Moreover, the evolutionary process of protein-coding genes was examined. Our results suggest that natural selection may have played a role for duplicated genes in both species, so that duplication was suppressed or favored in a manner that depended on the function of a gene.
ESTHER : Itoh_2007_Genome.Res_17_175
PubMedSearch : Itoh_2007_Genome.Res_17_175
PubMedID: 17210932
Gene_locus related to this paper: orysa-Q7XTC5 , orysa-Q852M6 , orysa-Q8GSE8 , orysa-Q9FYP7 , orysa-Q5ZA26 , orysa-Q5JLP6 , orysa-Q8H5P5 , orysa-Q7F1Y5 , orysa-cbp3 , orysa-cbpx , orysa-Q6YSZ8 , orysa-Q9FW17 , orysa-Q84QZ6 , orysa-Q0JK71 , orysa-B9EWJ8 , orysa-Q6ZDG6 , orysa-Q6ZDG5 , orysa-Q658B2 , orysa-Q5N7L1 , orysa-Q8RYV9 , orysa-Q8H3R3 , orysa-Q5SNH3 , orysa-pir7a , orysa-q2qnj4 , orysa-q2qyj1 , orysa-q2r077 , orysa-Q4VWY7 , orysa-q5smv5 , orysa-q5z901 , orysa-Q5ZBI5 , orysa-q6atz0 , orysa-q6i5q3 , orysa-q6j657 , orysa-q6k4q2 , orysj-q6yse8 , orysa-q6yy42 , orysa-q6yzk1 , orysa-q6z8b1 , orysa-q6z995 , orysa-q6zjq6 , orysa-q7x7y5 , orysa-Q7XC50 , orysa-q7xr62 , orysa-q7xr63 , orysa-q7xsg1 , orysa-q7xsq2 , orysa-q7xts6 , orysa-q7xv53 , orysa-Q8LQS5 , orysa-Q8RZ79 , orysa-Q8S0U8 , orysa-Q8W3C6 , orysa-Q9LHX5 , orysa-q53m20 , orysa-q53nd8 , orysa-q60e79 , orysa-q67iz2 , orysa-q67iz3 , orysa-q67iz7 , orysa-q67iz8 , orysa-q67j02 , orysa-q67j05 , orysa-q67j09 , orysa-q67j10 , orysa-q67tr6 , orysa-q67tv0 , orysa-q69j38 , orysa-q69y21 , orysa-q75hy1 , orysa-q75hy2 , orysa-Q0J0A4 , orysa-q651a8 , orysa-q652g4 , orysa-q688m8 , orysa-Q6H8G1 , orysi-a2z179 , orysi-a2zef2 , orysi-b8a7e6 , orysi-b8a7e7 , orysi-b8bfe5 , orysi-b8bhp9 , orysj-b9fi05 , orysj-q0djj0 , orysj-q0jaf0 , orysj-q0jga1 , orysj-q0jhi5 , orysj-q5jl22 , orysj-q5jlw7 , orysj-q6h7q9 , orysj-q6yvk6 , orysj-q7f8x1 , orysj-q7xcx3 , orysj-q9fwm6 , orysj-q10j20 , orysj-q10ss2 , orysj-q69uw6 , orysj-q94d71 , orysj-q0iq98 , orysj-b9gbs4 , orysj-b9gbs1

Title : Pancreatic pseudocyst after acute organophosphate poisoning - Kawabe_2006_Fukuoka.Igaku.Zasshi_97_123
Author(s) : Kawabe K , Ito T , Arita Y , Sadamoto Y , Harada N , Yamaguchi K , Tanaka M , Nakano I , Nawata H , Takayanagi R
Ref : Fukuoka Igaku Zasshi , 97 :123 , 2006
Abstract : Acute organophosphate poisoning (OP) shows several severe clinical symptoms due to its strong blocking effect on cholinesterase. Acute pancreatitis is one of the complications associated with acute OP, but this association still may not be widely recognized. We report here the case of a 73-year-old man who had repeated abdominal pain during and after the treatment of acute OP. Hyperamylasemia and a 7-cm pseudocyst in the pancreatic tail were noted on investigations. We diagnosed pancreatic pseudocyst that likely was secondary to an episode of acute pancreatitis following acute OP. He was initially treated with a long-term intravenous hyperalimentation, protease inhibitors and octerotide, but eventually required surgical intervention, a cystgastrostomy. Acute pancreatitis and hyperamylasemia are known to be possible complications of acute OP. It is necessary to examine and assess pancreatic damage in patients with acute OP.
ESTHER : Kawabe_2006_Fukuoka.Igaku.Zasshi_97_123
PubMedSearch : Kawabe_2006_Fukuoka.Igaku.Zasshi_97_123
PubMedID: 16850858

Title : The Rice Annotation Project Database (RAP-DB): hub for Oryza sativa ssp. japonica genome information - Ohyanagi_2006_Nucleic.Acids.Res_34_D741
Author(s) : Ohyanagi H , Tanaka T , Sakai H , Shigemoto Y , Yamaguchi K , Habara T , Fujii Y , Antonio BA , Nagamura Y , Imanishi T , Ikeo K , Itoh T , Gojobori T , Sasaki T
Ref : Nucleic Acids Research , 34 :D741 , 2006
Abstract : With the completion of the rice genome sequencing, a standardized annotation is necessary so that the information from the genome sequence can be fully utilized in understanding the biology of rice and other cereal crops. An annotation jamboree was held in Japan with the aim of annotating and manually curating all the genes in the rice genome. Here we present the Rice Annotation Project Database (RAP-DB), which has been developed to provide access to the annotation data. The RAP-DB has two different types of annotation viewers, BLAST and BLAT search, and other useful features. By connecting the annotations to other rice genomics data, such as full-length cDNAs and Tos17 mutant lines, the RAP-DB serves as a hub for rice genomics. All of the resources can be accessed through http://rapdb.lab.nig.ac.jp/.
ESTHER : Ohyanagi_2006_Nucleic.Acids.Res_34_D741
PubMedSearch : Ohyanagi_2006_Nucleic.Acids.Res_34_D741
PubMedID: 16381971
Gene_locus related to this paper: orysa-Q9FW17 , orysa-Q0JK71 , orysa-B9EWJ8 , orysa-Q5N7L1 , orysa-Q5N7J6 , orysa-pir7a , orysa-q2qyi1 , orysa-q2qyj1 , orysa-q2rbb3 , orysj-q6yse8 , orysa-q6yzk1 , orysa-Q8S0U8 , orysa-Q84ZY8 , orysa-Q0J0A4 , orysi-a2z179 , orysi-a2zef2 , orysi-b8a7e6 , orysi-b8a7e7 , orysi-b8bfe5 , orysi-b8bhp9 , orysj-b9fi05 , orysj-q0d4u5 , orysj-q0djj0 , orysj-q0jaf0 , orysj-q0jga1 , orysj-q0jhi5 , orysj-q5jl22 , orysj-q5jlw7 , orysj-q6h7q9 , orysj-q6yvk6 , orysj-q7f8x1 , orysj-q7xcx3 , orysj-q9fwm6 , orysj-q10j20 , orysj-q10ss2 , orysj-q69uw6 , orysj-q94d71 , orysj-q0iq98 , orysj-b9gbs4 , orysj-b9gbs1

Title : Role of leukotrienes on hepatic ischemia\/reperfusion injury in rats - Takamatsu_2004_J.Surg.Res_119_14
Author(s) : Takamatsu Y , Shimada K , Chijiiwa K , Kuroki S , Yamaguchi K , Tanaka M
Ref : J Surg Res , 119 :14 , 2004
Abstract : BACKGROUND: Leukotrienes (LT), composed of cysteinyl LT (cLT; LTC(4), LTD(4), and LTE(4)) and LTB(4), are potent lipid mediators enhancing the vascular permeability and recruitment of neutrophils, which are common features of hepatic ischemia/reperfusion (I/R) injury. The aim of this study was to investigate whether LT can mediate the liver and lung injuries following hepatic I/R. MATERIALS AND
METHODS: Sprague-Dawley rats were subjected to 90 min of partial hepatic ischemia followed by 3, 12, and 24 h of reperfusion. In the hepatic and pulmonary tissues, LT content and the mRNA expression of LT-synthesis enzymes, 5-lypoxygenase (5-LO), LTC(4) synthase (LTC(4)-S), and LTA(4) hydrolase (LTA(4)-H) were measured. Tissue injuries were assessed by plasma ALT, histological examination, and wet-to-dry tissue weight ratios.
RESULTS: The cLT content in the hepatic tissue after 12 and 24 h reperfusion was increased 4- to 5-fold compared to controls and this was accompanied by the enhancement of hepatic edema and plasma ALT elevation. There were no significant changes in the mRNA expression of LT-synthesis enzymes in both tissues. LTB(4) levels were not increased despite a significant neutrophil infiltration in both tissues.
CONCLUSIONS: These data suggest that cLT are generated in the liver during the reperfusion period and may contribute to the development of hepatic edema and exert cytotoxicity. Factors other than LTB(4) may contribute to neutrophil infiltration.
ESTHER : Takamatsu_2004_J.Surg.Res_119_14
PubMedSearch : Takamatsu_2004_J.Surg.Res_119_14
PubMedID: 15126076

Title : Determination of carbamate pesticide residues in vegetables and fruits by liquid chromatography-atmospheric pressure photoionization-mass spectrometry and atmospheric pressure chemical ionization-mass spectrometry - Takino_2004_J.Agric.Food.Chem_52_727
Author(s) : Takino M , Yamaguchi K , Nakahara T
Ref : Journal of Agricultural and Food Chemistry , 52 :727 , 2004
Abstract : A liquid chromatography-atmospheric pressure photoionization (APPI)-mass spectrometry method was developed for the determination of 22 carbamates including their metabolites in vegetables and fruits. For the optimization of APPI, several APPI ion source parameters were examined. As a result, many carbamates with APPI using the optimized parameter gave simple mass spectra, and a strong signal corresponding to [M + H](+) was observed except for aldicarb. However, some carbamate metabolites gave ammonium adduct ions [M + NH(4)](+) as base peak ions. The mean recovery of each carbamate from grape and onion samples spiked at 5 ng/g was 81.7-105.7%, with relative standard deviations of 3.3-5.9%. Furthermore, matrix constituents did not significantly influence the ionization efficiency. The limit of detection (S/N = 3) in grape and onion was in the range of 0.33-3.33 ng/g. For the robustness of this method, this system has been used to analyze 50 samples, and the intensities for all carbamates were found to be unaffected by the contamination of the APPI source by sample matrix constituents. This result indicates that the method is reliable.
ESTHER : Takino_2004_J.Agric.Food.Chem_52_727
PubMedSearch : Takino_2004_J.Agric.Food.Chem_52_727
PubMedID: 14969523