Shimada K

References (15)

Title : SARS-CoV-2 S1 protein causes brain inflammation by reducing intracerebral acetylcholine production - Oka_2023_iScience_26_106954
Author(s) : Oka N , Shimada K , Ishii A , Kobayashi N , Kondo K
Ref : iScience , 26 :106954 , 2023
Abstract : Neurological complications that occur in SARS-CoV-2 infection, such as olfactory dysfunction, brain inflammation, malaise, and depressive symptoms, are thought to contribute to long COVID. However, in autopsies of patients who have died from COVID-19, there is normally no direct evidence that central nervous system damage is due to proliferation of SARS-CoV-2. For this reason, many aspects of the pathogenesis mechanisms of such symptoms remain unknown. Expressing SARS-CoV-2 S1 protein in the nasal cavity of mice was associated with increased apoptosis of the olfactory system and decreased intracerebral acetylcholine production. The decrease in acetylcholine production was associated with brain inflammation, malaise, depressive clinical signs, and decreased expression of the cytokine degrading factor ZFP36. Administering the cholinesterase inhibitor donepezil to the mice improved brain inflammation, malaise and depressive clinical signs. These findings could contribute to the elucidation of the pathogenesis mechanisms of neurological complications associated with COVID-19 and long COVID.
ESTHER : Oka_2023_iScience_26_106954
PubMedSearch : Oka_2023_iScience_26_106954
PubMedID: 37275532

Title : Variants of carboxylesterase 1 have no impact on capecitabine pharmacokinetics and toxicity in capecitabine plus oxaliplatin treated-colorectal cancer patients - Matsumoto_2020_Cancer.Chemother.Pharmacol__
Author(s) : Matsumoto N , Kubota Y , Ishida H , Sekido M , Ohkuma R , Ishiguro T , Hirasawa Y , Ariizumi H , Tsunoda T , Ikusue T , Kobayashi K , Hisamatsu A , Toshima H , Shimada K , Fujita KI
Ref : Cancer Chemother Pharmacol , : , 2020
Abstract : PURPOSE: Capecitabine is a prodrug that undergoes metabolism in three steps to form an active 5-fluorouracil (5-FU). The first step is primarily catalyzed by liver carboxylesterases (CES) 1. Here, we examined the effects of CES1 variants on pharmacokinetics and toxicity of capecitabine. METHODS: We enrolled postoperative colorectal cancer (CRC) patients administered with adjuvant capecitabine plus oxaliplatin (CapeOX) and metastatic CRC patients receiving CapeOX. The pharmacokinetic analysis of the first capecitabine dose (1000 mg/m(2)) was done on day 1, and oxaliplatin administration was shifted to day 2. Plasma concentrations of capecitabine, 5'-deoxy-5-fluorocytidine, 5'-deoxy-5-fluorouridine (5'-DFUR), and 5-FU were analyzed by high-performance liquid chromatography. CES1 polymorphisms (rs3217164, rs2244614, rs2244613, rs7187684, and rs11861118) and the functional CES1 genes (1A1, var1A1, 1A2, and pseudo 1A3) in their diplotype configurations were analyzed by direct sequencing. RESULTS: Thirty-seven patients were enrolled from September 2017 to February 2020. Patients with a higher area under the plasma concentration-time curve to capecitabine dose ratio (AUC/dose) of 5'-DFUR than its mean showed a higher frequency of overall >/= grade 3 toxicity and lower relative dose intensity (RDI) of capecitabine than those with a lower ratio. Higher CES1 activity expressed as a metabolic ratio (AUC of capecitabine/sum of three AUCs of each metabolite) lower than its mean was associated with higher 5'-DFUR AUC/dose and lower RDI, indicating essential roles of CES1 in capecitabine activation to produce 5'-DFUR. However, the association between CES1 variants and capecitabine pharmacokinetics and toxicity was not significant. CONCLUSION: CES1 variants are not associated with capecitabine pharmacokinetics and toxicity.
ESTHER : Matsumoto_2020_Cancer.Chemother.Pharmacol__
PubMedSearch : Matsumoto_2020_Cancer.Chemother.Pharmacol__
PubMedID: 32458030

Title : Dissemination of 16S rRNA methylase ArmA-producing acinetobacter baumannii and emergence of OXA-72 carbapenemase coproducers in Japan - Tada_2014_Antimicrob.Agents.Chemother_58_2916
Author(s) : Tada T , Miyoshi-Akiyama T , Shimada K , Shimojima M , Kirikae T
Ref : Antimicrobial Agents & Chemotherapy , 58 :2916 , 2014
Abstract : Forty-nine clinical isolates of multidrug-resistant Acinetobacter baumannii were obtained from 12 hospitals in 7 prefectures throughout Japan. Molecular phylogenetic analysis revealed the clonal spread of A. baumannii sequence type 208 (ST208) and ST455 isolates harboring the armA gene and ST512 harboring the armA and blaOXA-72 genes. These findings show that A. baumannii isolates harboring armA are disseminated throughout Japan, and this is the first report to show that A. baumannii strains harboring blaOXA-72 and armA are emerging in hospitals in Japan.
ESTHER : Tada_2014_Antimicrob.Agents.Chemother_58_2916
PubMedSearch : Tada_2014_Antimicrob.Agents.Chemother_58_2916
PubMedID: 24550340
Gene_locus related to this paper: aciba-f5iht4 , aciba-a0a009wzt4

Title : Complete genome sequence of phototrophic betaproteobacterium Rubrivivax gelatinosus IL144 - Nagashima_2012_J.Bacteriol_194_3541
Author(s) : Nagashima S , Kamimura A , Shimizu T , Nakamura-Isaki S , Aono E , Sakamoto K , Ichikawa N , Nakazawa H , Sekine M , Yamazaki S , Fujita N , Shimada K , Hanada S , Nagashima KV
Ref : Journal of Bacteriology , 194 :3541 , 2012
Abstract : Rubrivivax gelatinosus is a facultative photoheterotrophic betaproteobacterium living in freshwater ponds, sewage ditches, activated sludge, and food processing wastewater. There have not been many studies on photosynthetic betaproteobacteria. Here we announce the complete genome sequence of the best-studied phototrophic betaproteobacterium, R. gelatinosus IL-144 (NBRC 100245).
ESTHER : Nagashima_2012_J.Bacteriol_194_3541
PubMedSearch : Nagashima_2012_J.Bacteriol_194_3541
PubMedID: 22689232
Gene_locus related to this paper: rubgi-i0hxc6

Title : Analysis of hunger-driven gene expression in the Drosophila melanogaster larval central nervous system - Ryuda_2008_Zoolog.Sci_25_746
Author(s) : Ryuda M , Shimada K , Koyanagi R , Azumi K , Tanimura T , Hayakawa Y
Ref : Zoolog Sci , 25 :746 , 2008
Abstract : A transposon-inserted mutant of Drosophila melanogaster was recently identified, and the larvae show no food preference (Ryuda and Hayakawa, 2005). To reveal the genetic mechanism underlying the preference change in this mutant, a large-scale oligo-DNA microarray screening was carried out to identify genes whose expression is different in control and mutant strains. We focused especially on hunger-driven changes in gene expression in the larval central nervous system (CNS) of both strains, because the state of food depletion should promote a feeding response due to changed expression of certain genes in the CNS. We identified 22 genes whose expression changed after starvation in either or both of the two strains. Quantitative RT-PCR analyses confirmed the expression changes in four genes, CG6271, CG6277, CG7953, and new glue 3 (ng3, encoding a putative structural molecule). CG6271 and CG6277 encode triacylglycerol lipase, and CG7953 produces a protein homologous to a juvenile hormone (JH) binding protein. The expression of these two groups of genes was enhanced in control strain larvae with a normal food preference but not in GS1189 strain larvae. Given that these genes contribute to mediating hunger-driven changes in food preference and intake in D. melanogaster larvae, the dysfunction of these key genes could cause the defect in food preference observed in GS1189-strain larvae.
ESTHER : Ryuda_2008_Zoolog.Sci_25_746
PubMedSearch : Ryuda_2008_Zoolog.Sci_25_746
PubMedID: 18828662

Title : Discovery and stereoselective synthesis of the novel isochroman neurokinin-1 receptor antagonist 'CJ-17,493' - Shishido_2008_Bioorg.Med.Chem_16_7193
Author(s) : Shishido Y , Wakabayashi H , Koike H , Ueno N , Nukui S , Yamagishi T , Murata Y , Naganeo F , Mizutani M , Shimada K , Fujiwara Y , Sakakibara A , Suga O , Kusano R , Ueda S , Kanai Y , Tsuchiya M , Satake K
Ref : Bioorganic & Medicinal Chemistry , 16 :7193 , 2008
Abstract : A novel central nervous system (CNS) selective neurokinin-1 (NK(1)) receptor antagonist, (2S,3S)-3-[(1R)-6-methoxy-1-methyl-1-trifluoromethylisochroman-7-yl]-methylamino- 2-phenylpiperidine 'CJ-17,493' (compound (+)-1), was synthesized stereoselectively using a kinetic resolution by lipase-PS as a key step. Compound (+)-1 displayed high and selective affinity (K(i)=0.2 nM) for the human NK(1) receptor in IM-9 cells, potent activity in the [Sar(9), Met(O(2))(11)]SP-induced gerbil tapping model (ED(50)=0.04 mg/kg, s.c.) and in the ferret cisplatin (10mg/kg, i.p.)-induced anti-emetic activity model (vomiting: ED(90)=0.07 mg/kg, s.c.), all levels of activity comparable with those of CP-122,721. In addition, compound (+)-1 exhibited linear pharmacokinetics rather than the super dose-proportionality of CP-122,721 and this result provides a potential solution for the clinical issue observed with CP-122,721.
ESTHER : Shishido_2008_Bioorg.Med.Chem_16_7193
PubMedSearch : Shishido_2008_Bioorg.Med.Chem_16_7193
PubMedID: 18640044

Title : The transcriptional landscape of the mammalian genome - Carninci_2005_Science_309_1559
Author(s) : Carninci P , Kasukawa T , Katayama S , Gough J , Frith MC , Maeda N , Oyama R , Ravasi T , Lenhard B , Wells C , Kodzius R , Shimokawa K , Bajic VB , Brenner SE , Batalov S , Forrest AR , Zavolan M , Davis MJ , Wilming LG , Aidinis V , Allen JE , Ambesi-Impiombato A , Apweiler R , Aturaliya RN , Bailey TL , Bansal M , Baxter L , Beisel KW , Bersano T , Bono H , Chalk AM , Chiu KP , Choudhary V , Christoffels A , Clutterbuck DR , Crowe ML , Dalla E , Dalrymple BP , de Bono B , Della Gatta G , di Bernardo D , Down T , Engstrom P , Fagiolini M , Faulkner G , Fletcher CF , Fukushima T , Furuno M , Futaki S , Gariboldi M , Georgii-Hemming P , Gingeras TR , Gojobori T , Green RE , Gustincich S , Harbers M , Hayashi Y , Hensch TK , Hirokawa N , Hill D , Huminiecki L , Iacono M , Ikeo K , Iwama A , Ishikawa T , Jakt M , Kanapin A , Katoh M , Kawasawa Y , Kelso J , Kitamura H , Kitano H , Kollias G , Krishnan SP , Kruger A , Kummerfeld SK , Kurochkin IV , Lareau LF , Lazarevic D , Lipovich L , Liu J , Liuni S , McWilliam S , Madan Babu M , Madera M , Marchionni L , Matsuda H , Matsuzawa S , Miki H , Mignone F , Miyake S , Morris K , Mottagui-Tabar S , Mulder N , Nakano N , Nakauchi H , Ng P , Nilsson R , Nishiguchi S , Nishikawa S , Nori F , Ohara O , Okazaki Y , Orlando V , Pang KC , Pavan WJ , Pavesi G , Pesole G , Petrovsky N , Piazza S , Reed J , Reid JF , Ring BZ , Ringwald M , Rost B , Ruan Y , Salzberg SL , Sandelin A , Schneider C , Schonbach C , Sekiguchi K , Semple CA , Seno S , Sessa L , Sheng Y , Shibata Y , Shimada H , Shimada K , Silva D , Sinclair B , Sperling S , Stupka E , Sugiura K , Sultana R , Takenaka Y , Taki K , Tammoja K , Tan SL , Tang S , Taylor MS , Tegner J , Teichmann SA , Ueda HR , van Nimwegen E , Verardo R , Wei CL , Yagi K , Yamanishi H , Zabarovsky E , Zhu S , Zimmer A , Hide W , Bult C , Grimmond SM , Teasdale RD , Liu ET , Brusic V , Quackenbush J , Wahlestedt C , Mattick JS , Hume DA , Kai C , Sasaki D , Tomaru Y , Fukuda S , Kanamori-Katayama M , Suzuki M , Aoki J , Arakawa T , Iida J , Imamura K , Itoh M , Kato T , Kawaji H , Kawagashira N , Kawashima T , Kojima M , Kondo S , Konno H , Nakano K , Ninomiya N , Nishio T , Okada M , Plessy C , Shibata K , Shiraki T , Suzuki S , Tagami M , Waki K , Watahiki A , Okamura-Oho Y , Suzuki H , Kawai J , Hayashizaki Y
Ref : Science , 309 :1559 , 2005
Abstract : This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development.
ESTHER : Carninci_2005_Science_309_1559
PubMedSearch : Carninci_2005_Science_309_1559
PubMedID: 16141072
Gene_locus related to this paper: mouse-abhd1 , mouse-abhd3 , mouse-abhd4 , mouse-acot4 , mouse-adcl4 , mouse-DGLB , mouse-ephx3 , mouse-Kansl3 , mouse-lipli , mouse-LIPN , mouse-Ppgb , mouse-q3uuq7 , mouse-srac1 , mouse-Tex30 , mouse-tmco4 , mouse-tmm53 , mouse-f172a

Title : Permethrin Resistance Mechanisms in the Beet Armyworm (Spodoptera exigua (Hubner)) - Shimada_2005_J.Pestic.Sci_30_214
Author(s) : Shimada K , Natsuhara K , Oomori Y , Miyata T
Ref : Journal of Pesticide Science , 30 :214 , 2005
Abstract : The toxicity of pyrethroids was evaluated in a permethrin-susceptible (TS) and a permethrin-resistant (TR) strain of the beet armyworm, Spodoptera exigua (Hubner). The TR-strain showed 92-fold more resistance to permethrin and higher cross-resistance (97- and 130-fold, respectively) to cypermethrin and fenvalerate than the TS-strain. Moreover, all larval instars exhibited greater susceptibility to permethrin in the TS-strain than TR-strain. There was very little difference in susceptibility between the two strains with respect to chlorphenapyr. The effect of piperonyl butoxide on the toxicity of permethrin indicated that the resistance of the TR-strain is due to enhanced metabolic detoxification by cytochrome P450 monooxygenase
ESTHER : Shimada_2005_J.Pestic.Sci_30_214
PubMedSearch : Shimada_2005_J.Pestic.Sci_30_214
PubMedID:

Title : Role of leukotrienes on hepatic ischemia\/reperfusion injury in rats - Takamatsu_2004_J.Surg.Res_119_14
Author(s) : Takamatsu Y , Shimada K , Chijiiwa K , Kuroki S , Yamaguchi K , Tanaka M
Ref : J Surg Res , 119 :14 , 2004
Abstract : BACKGROUND: Leukotrienes (LT), composed of cysteinyl LT (cLT; LTC(4), LTD(4), and LTE(4)) and LTB(4), are potent lipid mediators enhancing the vascular permeability and recruitment of neutrophils, which are common features of hepatic ischemia/reperfusion (I/R) injury. The aim of this study was to investigate whether LT can mediate the liver and lung injuries following hepatic I/R. MATERIALS AND
METHODS: Sprague-Dawley rats were subjected to 90 min of partial hepatic ischemia followed by 3, 12, and 24 h of reperfusion. In the hepatic and pulmonary tissues, LT content and the mRNA expression of LT-synthesis enzymes, 5-lypoxygenase (5-LO), LTC(4) synthase (LTC(4)-S), and LTA(4) hydrolase (LTA(4)-H) were measured. Tissue injuries were assessed by plasma ALT, histological examination, and wet-to-dry tissue weight ratios.
RESULTS: The cLT content in the hepatic tissue after 12 and 24 h reperfusion was increased 4- to 5-fold compared to controls and this was accompanied by the enhancement of hepatic edema and plasma ALT elevation. There were no significant changes in the mRNA expression of LT-synthesis enzymes in both tissues. LTB(4) levels were not increased despite a significant neutrophil infiltration in both tissues.
CONCLUSIONS: These data suggest that cLT are generated in the liver during the reperfusion period and may contribute to the development of hepatic edema and exert cytotoxicity. Factors other than LTB(4) may contribute to neutrophil infiltration.
ESTHER : Takamatsu_2004_J.Surg.Res_119_14
PubMedSearch : Takamatsu_2004_J.Surg.Res_119_14
PubMedID: 15126076

Title : Phenobarbital induction of permethrin detoxification and phenobarbital metabolism in susceptible and resistant strains of the beet armyworm Spodoptera exigua (Hubner) - Natsuhara_2004_Pestic.Biochem.Physiol_79_33
Author(s) : Natsuhara K , Shimada K , Tanaka T , Miyata T
Ref : Pesticide Biochemistry and Physiology , 79 :33 , 2004
Abstract : The permethrin resistant strain (TR-strain) of the beet armyworm, Spodoptera exigua (Hbner), has 92.5-fold resistance to permethrin (at LD50 level) compared to the permethrin susceptible strain (TS-strain). Bioassay involving permethrin mixed with piperonyl butoxide, an inhibitor of microsomal cytochrome P450s, significantly reduced the resistance ratio from 92.5- to 7.9-fold. However, S,S,S-tributylphosphorotrithioate and diethylmaleate which are inhibitors of esterases and glutathione S-transferase, respectively, did not affect the resistance level. These results indicate that the detoxification of permethrin in the TR-strain was primarily due to the cytochrome P450 monooxygenases. LD50 for permethrin was increased to 4.5-fold by the pre-treatment of phenobarbital in the TS-strain. The effect of induction by phenobarbital was almost completely overcome by the piperonyl butoxide treatment. However, it was observed that phenobarbital treatment did not cause any change in the toxicity of permethrin to TR strain. Since this result deviated from the expectation that the metabolism of phenobarbital in the TR-strain should be greater than that in the TS-strain, it was deemed necessary to compare the metabolism of phenobarbital between the TS- and TR-strains. Comparison was made based on the concentration of phenobarbital in the hemolymph and whole body. The results showed no significant difference in phenobarbital treatment between the two strains used in this study suggesting the possibility that the induction system in TS-strain is different from the TR-strain.
ESTHER : Natsuhara_2004_Pestic.Biochem.Physiol_79_33
PubMedSearch : Natsuhara_2004_Pestic.Biochem.Physiol_79_33
PubMedID:

Title : Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs - Okazaki_2002_Nature_420_563
Author(s) : Okazaki Y , Furuno M , Kasukawa T , Adachi J , Bono H , Kondo S , Nikaido I , Osato N , Saito R , Suzuki H , Yamanaka I , Kiyosawa H , Yagi K , Tomaru Y , Hasegawa Y , Nogami A , Schonbach C , Gojobori T , Baldarelli R , Hill DP , Bult C , Hume DA , Quackenbush J , Schriml LM , Kanapin A , Matsuda H , Batalov S , Beisel KW , Blake JA , Bradt D , Brusic V , Chothia C , Corbani LE , Cousins S , Dalla E , Dragani TA , Fletcher CF , Forrest A , Frazer KS , Gaasterland T , Gariboldi M , Gissi C , Godzik A , Gough J , Grimmond S , Gustincich S , Hirokawa N , Jackson IJ , Jarvis ED , Kanai A , Kawaji H , Kawasawa Y , Kedzierski RM , King BL , Konagaya A , Kurochkin IV , Lee Y , Lenhard B , Lyons PA , Maglott DR , Maltais L , Marchionni L , McKenzie L , Miki H , Nagashima T , Numata K , Okido T , Pavan WJ , Pertea G , Pesole G , Petrovsky N , Pillai R , Pontius JU , Qi D , Ramachandran S , Ravasi T , Reed JC , Reed DJ , Reid J , Ring BZ , Ringwald M , Sandelin A , Schneider C , Semple CA , Setou M , Shimada K , Sultana R , Takenaka Y , Taylor MS , Teasdale RD , Tomita M , Verardo R , Wagner L , Wahlestedt C , Wang Y , Watanabe Y , Wells C , Wilming LG , Wynshaw-Boris A , Yanagisawa M , Yang I , Yang L , Yuan Z , Zavolan M , Zhu Y , Zimmer A , Carninci P , Hayatsu N , Hirozane-Kishikawa T , Konno H , Nakamura M , Sakazume N , Sato K , Shiraki T , Waki K , Kawai J , Aizawa K , Arakawa T , Fukuda S , Hara A , Hashizume W , Imotani K , Ishii Y , Itoh M , Kagawa I , Miyazaki A , Sakai K , Sasaki D , Shibata K , Shinagawa A , Yasunishi A , Yoshino M , Waterston R , Lander ES , Rogers J , Birney E , Hayashizaki Y
Ref : Nature , 420 :563 , 2002
Abstract : Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the mouse, and to ensure that each is represented in a physical collection of clones. Here we report the manual annotation of 60,770 full-length mouse complementary DNA sequences. These are clustered into 33,409 'transcriptional units', contributing 90.1% of a newly established mouse transcriptome database. Of these transcriptional units, 4,258 are new protein-coding and 11,665 are new non-coding messages, indicating that non-coding RNA is a major component of the transcriptome. 41% of all transcriptional units showed evidence of alternative splicing. In protein-coding transcripts, 79% of splice variations altered the protein product. Whole-transcriptome analyses resulted in the identification of 2,431 sense-antisense pairs. The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics.
ESTHER : Okazaki_2002_Nature_420_563
PubMedSearch : Okazaki_2002_Nature_420_563
PubMedID: 12466851
Gene_locus related to this paper: mouse-1lipg , mouse-1llip , mouse-1plrp , mouse-3neur , mouse-ABH15 , mouse-abhd4 , mouse-abhd5 , mouse-Abhd8 , mouse-Abhd11 , mouse-abhda , mouse-acot4 , mouse-adcl4 , mouse-AI607300 , mouse-BAAT , mouse-bphl , mouse-C87498 , mouse-Ldah , mouse-Ces1d , mouse-Ces2e , mouse-CMBL , mouse-DGLB , mouse-dpp9 , mouse-ES10 , mouse-F135A , mouse-FASN , mouse-hslip , mouse-hyes , mouse-Kansl3 , mouse-LIPH , mouse-LIPK , mouse-lipli , mouse-LIPM , mouse-lypla1 , mouse-lypla2 , mouse-MEST , mouse-MGLL , mouse-ndr4 , mouse-OVCA2 , mouse-pafa , mouse-pcp , mouse-ppce , mouse-Ppgb , mouse-PPME1 , mouse-q3uuq7 , mouse-Q8BLF1 , mouse-ACOT6 , mouse-Q8C1A9 , mouse-Q9DAI6 , mouse-Q80UX8 , mouse-Q8BGG9 , mouse-Q8C167 , mouse-rbbp9 , mouse-SERHL , mouse-tssp

Title : [Simultaneous determination of propanil, carbaryl and 3,4-dichloroaniline in human serum by HPLC with UV detector following solid phase extraction] - Hori_2002_Yakugaku.Zasshi_122_247
Author(s) : Hori Y , Nakajima M , Fujisawa M , Shimada K , Hirota T , Yoshioka T
Ref : Yakugaku Zasshi , 122 :247 , 2002
Abstract : In case of poisoning by herbicide compounded with Propanil (DCPA) and Carbaryl (NAC), we attempted simultaneous solid-phase extractions of DCPA, NAC, and 3,4-dichloroaniline (DCA), a metabolite of DCPA, from the patient's serum, and quantitative analytical method using HPLC-UV detection. With this HPLC method, the quantitative detection limits in the serum are 0.005 microgram/ml for DCPA and DCA and 0.001 microgram/ml for NAC, and the UV spectra of all three compounds could easily be obtained using a diode-array detection limit of 0.05 microgram/ml. When the three compounds were added to serum at concentrations ranging from 0.1-10.0 micrograms/ml, the recovery rates were satisfactory at between 91.1% and 101.9%. On analysis of the serum of patient who had ingested Kusanon A Emulsion, the ingested substance apparently caused an increase in the DCA concentration, which led to the appearance of methemoglobinemia. The possibility that the DCA concentration might be used for prognostic purposes was suggested.
ESTHER : Hori_2002_Yakugaku.Zasshi_122_247
PubMedSearch : Hori_2002_Yakugaku.Zasshi_122_247
PubMedID: 11905049

Title : The mechanism responsible for the drowsiness caused by first generation H1 antagonists on the EEG pattern - Kaneko_2000_Methods.Find.Exp.Clin.Pharmacol_22_163
Author(s) : Kaneko Y , Shimada K , Saitou K , Sugimoto Y , Kamei C
Ref : Methods Find Exp Clin Pharmacol , 22 :163 , 2000
Abstract : The present study was performed to clarify the mechanism responsible for the drowsiness caused by first generation H1 antagonists according to electroencephalogram activity. All H1 antagonists used in the present study caused the EEG-recorded drowsiness pattern, i.e., increases in EEG power spectra of the delta and theta bands at the frontal cortex in rats. The potency of cyproheptadine was greater than those of diphenhydramine and promethazine, while that of pyrilamine was less than those of the other drugs examined. The increase in EEG power spectra in the delta band induced by H1 antagonists was antagonized by pretreatment with both histidine and physostigmine. The effect of pyrilamine was more potently antagonized by histidine and less potently antagonized by physostigmine as compared to diphenhydramine, promethazine and cyproheptadine. The increases in EEG power spectra induced by H1 antagonists were neither antagonized nor potentiated by 5-hydroxytryptophan. These results clearly indicate that the increases in EEG power spectra in the delta and theta bands at the frontal cortex in rats induced by first generation H1 antagonists are responsible for both histaminergic and cholinergic mechanisms.
ESTHER : Kaneko_2000_Methods.Find.Exp.Clin.Pharmacol_22_163
PubMedSearch : Kaneko_2000_Methods.Find.Exp.Clin.Pharmacol_22_163
PubMedID: 10893699

Title : Primary structure and transcription of genes encoding B870 and photosynthetic reaction center apoproteins from Rubrivivax gelatinosus - Nagashima_1994_J.Biol.Chem_269_2477
Author(s) : Nagashima KV , Matsuura K , Ohyama S , Shimada K
Ref : Journal of Biological Chemistry , 269 :2477 , 1994
Abstract : We determined the nucleotide sequence of the puf operon of Rubrivivax gelatinosus (formerly called Rhodocyclus gelatinosus), a photosynthetic bacterium belonging to the beta subclass of purple bacteria (proteobacteria). The operon contains two unknown open reading frames (ORFs) in addition to five photosynthetic genes which have been reported in species belonging to the alpha subclass. These genes include pufB, -A, -L, -M, and -C coding for the beta and alpha subunits of the B870 light-harvesting protein and for the L, M, and cytochrome subunits of the reaction center complex, respectively. One (ORF1) of the two unknown ORFs locates immediately upstream of pufB, while the other (ORF2) locates between pufB and pufA. The deduced product of ORF1 is a small basic protein composed of 41 amino acids that did not show homology with any other proteins reported to date. The deduced product of ORF2 was composed of 74 amino acids having a hydrophobic region able to span the membrane and showed homology with the beta subunit of B870. The deduced amino acid sequence of the cytochrome subunit of the reaction center of R. gelatinosus had significant deletions in the possible attachment site to the M subunit compared with that of Rhodopseudomonas viridis. This is consistent with the facilitated dissociation of this subunit observed with detergent treatment in R. gelatinosus. Analysis of RNA from R. gelatinosus showed that the puf operon of this bacterium has two transcripts; the major one is 1 kilobase in length and the minor one is 4 kilobase in length. These two transcripts have the same 5'-end, and their stoichiometry appeared to be controlled by a putative hairpin structure between pufA and pufL.
ESTHER : Nagashima_1994_J.Biol.Chem_269_2477
PubMedSearch : Nagashima_1994_J.Biol.Chem_269_2477
PubMedID: 8300574
Gene_locus related to this paper: rhoge-Q9JPD4

Title : Phylogenetic analysis of photosynthetic genes of Rhodocyclus gelatinosus: Possibility of horizontal gene transfer in purple bacteria - Nagashima_1993_Photosynth.Res_36_185
Author(s) : Nagashima KV , Shimada K , Matsuura K
Ref : Photosynth Res , 36 :185 , 1993
Abstract : Nucleotide sequences of the genes coding for the M and cytochrome subunits of the photosynthetic reaction center of Rhodocyclus gelatinosus, a purple bacterium in the beta subdivision, were determined. The deduced amino acid sequences of these proteins were compared with those of other photosynthetic bacteria. Based on the homology of these two photosynthetic proteins, Rc. gelatinosus was placed in the alpha subdivision of purple bacteria, which disagrees with the phylogenetic trees based on 16S rRNA and soluble cytochrome c 2. Horizontal transfer of the genes which code for the photosynthetic apparatus in purple bacteria can be postulated if the phylogenetic trees based on 16S rRNA and soluble cytochrome c 2 reflect the real history of purple bacteria.
ESTHER : Nagashima_1993_Photosynth.Res_36_185
PubMedSearch : Nagashima_1993_Photosynth.Res_36_185
PubMedID: 24318922
Gene_locus related to this paper: rhoge-Q9JPD4