Sal-A-alkyne
After the Sal alk probe labeling of proteins in P. falciparum, lysates of saponin-isolated parasites are prepared and labeled proteins are conjugated with TAMRA-azide using a CLICK reaction. This gives labelled proteins that can be separated on gel.
General
Type : Organophosphate, Cyclic OP, Lipase inhibitor, ABPP-Probe, Alkyne
Chemical_Nomenclature :
Canonical SMILES : C#CCCCCCCCCCCCCCO[P]1(OC[C@H]2C(=C(O1)CCCC)C(OC2)=O)=O
InChI : InChI=1S\/C25H41O6P\/c1-3-5-7-8-9-10-11-12-13-14-15-16-17-19-29-32(27)30-21-22-20-28-25(26)24(22)23(31-32)18-6-4-2\/h1,22H,4-21H2,2H3\/t22-,32?\/m0\/s1
InChIKey : SENYZAIVPYARCL-LCUARMQBSA-N
Other name(s) :
Target
Families : Sal-A-alkyne ligand of proteins in family
Plasmodium_subtelomeric_PST-A
ABHD13-BEM46
Duf_676
PGAP1
ABHD17-depalmitoylase
Protein :
plafa-PF10.0020
plafa-PF07.0040
plafa-a0a143zya4
plafa-PF10.0379
plafa-PF13.0153
plaf7-q8iik5
plaf7-q8ii19
plafa-MAL8P1.138
References (1)
| Title : The Antimalarial Natural Product Salinipostin A Identifies Essential alpha\/beta Serine Hydrolases Involved in Lipid Metabolism in P. falciparum Parasites - Yoo_2020_Cell.Chem.Biol_27_143 |
| Author(s) : Yoo E , Schulze CJ , Stokes BH , Onguka O , Yeo T , Mok S , Gnadig NF , Zhou Y , Kurita K , Foe IT , Terrell SM , Boucher MJ , Cieplak P , Kumpornsin K , Lee MCS , Linington RG , Long JZ , Uhlemann AC , Weerapana E , Fidock DA , Bogyo M |
| Ref : Cell Chemical Biology , 27 :143 , 2020 |
| Abstract : |
| PubMedSearch : Yoo_2020_Cell.Chem.Biol_27_143 |
| PubMedID: 31978322 |
| Gene_locus related to this paper: plaf7-q8ii19 , plafa-a0a143zya4 , plaf7-q8iik5 , plafa-MAL8P1.38 , plafa-PF07.0040 , plafa-PF10.0020 , plafa-PF10.0379 , plafa-PF13.0153 |