9bact-LAE6
Uncultured bacterium esterase LAE6 EH1 EH1AB1 alpha/beta hydrolase enzyme from the metagenome of Lake Arreo, Spain
Comment
EH1A promiscuous activity 72\/96 substrates Martinez-Martinez et al. 2018<\/A> || . Using Ser211 as nucleophile computationally designed additional mutations, adding Glu25Asp and Leu214His double mutant gives an additional active site with Gly207, Tyr208 and Phe209 act as a potential oxyanion hole Santiagoet al. 2018
Relationship
Family : Hormone-sensitive_lipase_like
Block : H
Position in NCBI Life Tree : uncultured bacterium
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > environmental samples N E > uncultured bacterium N E > cellular organisms N E > Bacteria N E > environmental samples N E > uncultured bacterium N E > cellular organisms N E > Bacteria N E > environmental samples N E > uncultured bacterium N EMolecular evidence
No mutation
5JD4 - Crystal structure of LAE6, an enzyme from the metagenome of Lake Arreo, Spain SRA059294
5JD4 - Crystal structure of LAE6, an enzyme from the metagenome of Lake Arreo, Spain SRA059294
5JD4 - Crystal structure of LAE6, an enzyme from the metagenome of Lake Arreo, Spain SRA059294
6I8D - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo complexed with a derivative of butyl 4-nitrophenyl hexylphosphonate
6I8D - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo complexed with a derivative of butyl 4-nitrophenyl hexylphosphonate
6I8D - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo complexed with a derivative of butyl 4-nitrophenyl hexylphosphonate
6I8F - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo
6I8F - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo
6I8F - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo
6RB0 - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo complexed with a derivative of methyl 4-nitrophenyl hexylphosphonate
6RB0 - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo complexed with a derivative of methyl 4-nitrophenyl hexylphosphonate
6RB0 - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo complexed with a derivative of methyl 4-nitrophenyl hexylphosphonate
6RKY - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo complexed with a derivative of bipyridine phosphonate
6RKY - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo complexed with a derivative of bipyridine phosphonate
6RKY - Structure of ester-hydrolase EH1AB1 from the metagenome of lake Arreo complexed with a derivative of bipyridine phosphonate
No kinetic
No disease
No substrate
Database
Sequence
Peptide
MLLPETRNLL DLMDAATRGG RPRLETLPHA VGRKAVDKMS EDGEADPPEV AEVANGGFAG PASEIRFRRY RPLGEAAGLL PTLIYYHGGG FVIGNIETHD STCRRLANKS RCQVISIDYR LAPEHPFPAP IDDGIAAFRH IRDNAESFGA DAARLAVGGD SAGGAMAAVV CQACRDAGET GPAFQMLIYP ATDSSRESAS RVAFAEGYFL SKALMDWFWE AYVPEDTDLT DLRLSPLLAT DFTGLPPAFV LTAGYDPLRD EGRAYADRLI EAGIKTTYVN YPGTIHGFFS LTRFLSQGLK ANDEAAAVMG AHFGT
References (12)
| Title : Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis - Alonso_2020_Nat.Catal_3_319 |
| Author(s) : Alonso S , Santiago G , Cea-Rama I , Fernandez-Lopez L , Coscolin C , Modregger J , Ressmann A , Martinez-Martinez M , Marrero H , Bargiela R , Pita M , Gonzalez-Alfonso JL , Briand M , Rojo D , Barbas C , Plou FJ , Golyshin PN , Shahgaldian P , Sanz-Aparicio J , Guallar V , Ferrer M |
| Ref : Nature Catalysis , 3 :319 , 2019 |
| Abstract : |
| PubMedSearch : Alonso_2020_Nat.Catal_3_319 |
| PubMedID: |
| Gene_locus related to this paper: 9bact-LAE6 |
| Title : Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis - Alonso_2020_Nat.Catal_3_319 |
| Author(s) : Alonso S , Santiago G , Cea-Rama I , Fernandez-Lopez L , Coscolin C , Modregger J , Ressmann A , Martinez-Martinez M , Marrero H , Bargiela R , Pita M , Gonzalez-Alfonso JL , Briand M , Rojo D , Barbas C , Plou FJ , Golyshin PN , Shahgaldian P , Sanz-Aparicio J , Guallar V , Ferrer M |
| Ref : Nature Catalysis , 3 :319 , 2019 |
| Abstract : |
| PubMedSearch : Alonso_2020_Nat.Catal_3_319 |
| PubMedID: |
| Gene_locus related to this paper: 9bact-LAE6 |
| Title : Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis - Alonso_2020_Nat.Catal_3_319 |
| Author(s) : Alonso S , Santiago G , Cea-Rama I , Fernandez-Lopez L , Coscolin C , Modregger J , Ressmann A , Martinez-Martinez M , Marrero H , Bargiela R , Pita M , Gonzalez-Alfonso JL , Briand M , Rojo D , Barbas C , Plou FJ , Golyshin PN , Shahgaldian P , Sanz-Aparicio J , Guallar V , Ferrer M |
| Ref : Nature Catalysis , 3 :319 , 2019 |
| Abstract : |
| PubMedSearch : Alonso_2020_Nat.Catal_3_319 |
| PubMedID: |
| Gene_locus related to this paper: 9bact-LAE6 |
| Title : Rational Engineering of Multiple Active Sites in an Ester Hydrolase - Santiago_2018_Biochemistry_57_2245 |
| Author(s) : Santiago G , Martinez-Martinez M , Alonso S , Bargiela R , Coscolin C , Golyshin PN , Guallar V , Ferrer M |
| Ref : Biochemistry , 57 :2245 , 2018 |
| Abstract : |
| PubMedSearch : Santiago_2018_Biochemistry_57_2245 |
| PubMedID: 29600855 |
| Gene_locus related to this paper: 9bact-LAE6 |
| Title : Rational Engineering of Multiple Active Sites in an Ester Hydrolase - Santiago_2018_Biochemistry_57_2245 |
| Author(s) : Santiago G , Martinez-Martinez M , Alonso S , Bargiela R , Coscolin C , Golyshin PN , Guallar V , Ferrer M |
| Ref : Biochemistry , 57 :2245 , 2018 |
| Abstract : |
| PubMedSearch : Santiago_2018_Biochemistry_57_2245 |
| PubMedID: 29600855 |
| Gene_locus related to this paper: 9bact-LAE6 |
| Title : Rational Engineering of Multiple Active Sites in an Ester Hydrolase - Santiago_2018_Biochemistry_57_2245 |
| Author(s) : Santiago G , Martinez-Martinez M , Alonso S , Bargiela R , Coscolin C , Golyshin PN , Guallar V , Ferrer M |
| Ref : Biochemistry , 57 :2245 , 2018 |
| Abstract : |
| PubMedSearch : Santiago_2018_Biochemistry_57_2245 |
| PubMedID: 29600855 |
| Gene_locus related to this paper: 9bact-LAE6 |
| Title : Biochemical diversity of carboxyl esterases and lipases from lake arreo (Spain): a metagenomic approach - Martinez-Martinez_2013_Appl.Environ.Microbiol_79_3553 |
| Author(s) : Martinez-Martinez M , Alcaide M , Tchigvintsev A , Reva O , Polaina J , Bargiela R , Guazzaroni ME , Chicote A , Canet A , Valero F , Rico Eguizabal E , Guerrero Mdel C , Yakunin AF , Ferrer M |
| Ref : Applied Environmental Microbiology , 79 :3553 , 2013 |
| Abstract : |
| PubMedSearch : Martinez-Martinez_2013_Appl.Environ.Microbiol_79_3553 |
| PubMedID: 23542620 |
| Gene_locus related to this paper: 9bact-LAE6 |
| Title : Biochemical diversity of carboxyl esterases and lipases from lake arreo (Spain): a metagenomic approach - Martinez-Martinez_2013_Appl.Environ.Microbiol_79_3553 |
| Author(s) : Martinez-Martinez M , Alcaide M , Tchigvintsev A , Reva O , Polaina J , Bargiela R , Guazzaroni ME , Chicote A , Canet A , Valero F , Rico Eguizabal E , Guerrero Mdel C , Yakunin AF , Ferrer M |
| Ref : Applied Environmental Microbiology , 79 :3553 , 2013 |
| Abstract : |
| PubMedSearch : Martinez-Martinez_2013_Appl.Environ.Microbiol_79_3553 |
| PubMedID: 23542620 |
| Gene_locus related to this paper: 9bact-LAE6 |
| Title : Biochemical diversity of carboxyl esterases and lipases from lake arreo (Spain): a metagenomic approach - Martinez-Martinez_2013_Appl.Environ.Microbiol_79_3553 |
| Author(s) : Martinez-Martinez M , Alcaide M , Tchigvintsev A , Reva O , Polaina J , Bargiela R , Guazzaroni ME , Chicote A , Canet A , Valero F , Rico Eguizabal E , Guerrero Mdel C , Yakunin AF , Ferrer M |
| Ref : Applied Environmental Microbiology , 79 :3553 , 2013 |
| Abstract : |
| PubMedSearch : Martinez-Martinez_2013_Appl.Environ.Microbiol_79_3553 |
| PubMedID: 23542620 |
| Gene_locus related to this paper: 9bact-LAE6 |