Jian J

References (11)

Title : High-Resolution Bioassay Profiling with Complemented Sensitivity and Resolution for Pancreatic Lipase Inhibitor Screening - Jian_2022_Molecules_27_
Author(s) : Jian J , Yuan J , Fan Y , Wang J , Zhang T , Kool J , Jiang Z
Ref : Molecules , 27 : , 2022
Abstract : How to rapidly and accurately screen bioactive components from complex natural products remains a major challenge. In this study, a screening platform for pancreatic lipase (PL) inhibitors was established by combining magnetic beads-based ligand fishing and high-resolution bioassay profiling. This platform was well validated using a mixture of standard compounds, i.e., (-)- epigallocatechin gallate (EGCG), luteolin and schisandrin. The dose-effect relationship of high-resolution bioassay profiling was demonstrated by the standard mixture with different concentrations for each compound. The screening of PL inhibitors from green tea extract at the concentrations of 0.2, 0.5 and 1.0 mg/mL by independent high-resolution bioassay profiling was performed. After sample pre-treatment by ligand fishing, green tea extract at the concentration of 0.2 mg/mL was specifically enriched and simplified, and consequently screened through the high-resolution bioassay profiling. As a result, three PL inhibitors, i.e., EGCG, (-)-Gallocatechin gallate (GCG) and (-)-Epicatechin gallate (ECG), were rapidly identified from the complex matrix. The established platform proved to be capable of enriching affinity binders and eliminating nonbinders in sample pre-treatment by ligand fishing, which overcame the technical challenges of high-resolution bioassay profiling in the aspects of sensitivity and resolution. Meanwhile, the high-resolution bioassay profiling possesses the ability of direct bioactive assessment, parallel structural analysis and identification after separation. The established platform allowed more accurate and rapid screening of PL inhibitors, which greatly facilitated natural product-based drug screening.
ESTHER : Jian_2022_Molecules_27_
PubMedSearch : Jian_2022_Molecules_27_
PubMedID: 36296516

Title : m(6) A transferase METTL3-induced lncRNA ABHD11-AS1 promotes the Warburg effect of non-small-cell lung cancer - Xue_2021_J.Cell.Physiol_236_2649
Author(s) : Xue L , Li J , Lin Y , Liu D , Yang Q , Jian J , Peng J
Ref : Journal of Cellular Physiology , 236 :2649 , 2021
Abstract : N(6) -methyladenosine (m(6) A) and long noncoding RNAs (lncRNAs) are both crucial regulators in non-small-cell lung cancer (NSCLC) tumorigenesis. However, the pathological roles of m(6) A and lncRNAs in NSCLC progression are still limited and undefined. Here, lncRNA ABHD11-AS1 was upregulated in NSCLC tissue specimens and cells and the ectopic overexpression was closely correlated with unfavorable prognosis of NSCLC patients. Functionally, ABHD11-AS1 promoted the proliferation and Warburg effect of NSCLC. Mechanistically, m(6) A profile was analyzed by methylated RNA immunoprecipitation sequencing (MeRIP-Seq). MeRIP-Seq presented that there was m(6) A modification site in ABHD11-AS1. m(6) A methyltransferase-like 3 (METTL3) installed the m(6) A modification and enhanced ABHD11-AS1 transcript stability to increase its expression. In conclusion, our findings highlight the function and mechanism of METTL3-induced ABHD11-AS1 in NSCLC and inspire the understanding of m(6) A and lncRNA in cancer biology.
ESTHER : Xue_2021_J.Cell.Physiol_236_2649
PubMedSearch : Xue_2021_J.Cell.Physiol_236_2649
PubMedID: 32892348
Gene_locus related to this paper: human-ABHD11

Title : An overview on synthesis, properties and applications of poly(butylene-adipate-co-terephthalate)-PBAT - Jian_2020_Adv.Industrial.Eng.Polym.Res_3_19
Author(s) : Jian J , Xiangbin Z , Xianbo H
Ref : Adv Industrial Eng Polym Res , 3 :19 , 2020
Abstract : A significantly growing interest is to design new biodegradable polymers in order to solve fossil resources and environmental pollution problems associated with conventional plastics. A kind of new biodegradable polymers, aliphatic-aromatic co-polyesters have been researched widely and developed rapidly in recent years, since that can combine excellent biodegradability provided from aliphatic polyesters and good properties from aromatic polyesters. Out of which, poly (butylene-adipate-co-terephthalate) (PBAT) shows the most importance. PBAT has been commercialized by polycondensation reaction of butanediol (BDO), adipic acid (AA) and terephthalic acid (PTA) using general polyester manufacturing technology and it has been considered to have desirable properties and competitive costs to be applied in many fields. Therefore, this review aims to present an overview on the synthesis, properties and applications of PBAT.
ESTHER : Jian_2020_Adv.Industrial.Eng.Polym.Res_3_19
PubMedSearch : Jian_2020_Adv.Industrial.Eng.Polym.Res_3_19

Title : Molecular footprints of inshore aquatic adaptation in Indo-Pacific humpback dolphin (Sousa chinensis) - Ming_2019_Genomics_111_1034
Author(s) : Ming Y , Jian J , Yu F , Yu X , Wang J , Liu W
Ref : Genomics , 111 :1034 , 2019
Abstract : The Indo-Pacific humpback dolphin, Sousa chinensis, being a member of cetaceans, had fully adapted to inshore waters. As a threatened marine mammal, little molecular information available for understanding the genetic basis of ecological adaptation. We firstly sequenced and obtained the draft genome map of S. chinensis. Phylogenetic analysis in this study, based on the single copy orthologous genes of the draft genome, is consistent with traditional phylogenetic classification. The comparative genomic analysis indicated that S. chinensis had 494 species-specific gene families, which involved immune, DNA repair and sensory systems associated with the potential adaption mechanism. We also identified the expansion and positive selection genes in S. chinensis lineage to investigate the potential adaptation mechanism. Our study provided the potential insight into the molecular bases of ecological adaptation in Indo-Pacific humpback dolphin and will be also valuable for future understanding the ecological adaptation and evolution of cetaceans at the genomic level.
ESTHER : Ming_2019_Genomics_111_1034
PubMedSearch : Ming_2019_Genomics_111_1034
PubMedID: 30031902
Gene_locus related to this paper: delle-a0a2y9mw48

Title : Draft genome sequence of Camellia sinensis var. sinensis provides insights into the evolution of the tea genome and tea quality - Wei_2018_Proc.Natl.Acad.Sci.U.S.A_115_E4151
Author(s) : Wei C , Yang H , Wang S , Zhao J , Liu C , Gao L , Xia E , Lu Y , Tai Y , She G , Sun J , Cao H , Tong W , Gao Q , Li Y , Deng W , Jiang X , Wang W , Chen Q , Zhang S , Li H , Wu J , Wang P , Li P , Shi C , Zheng F , Jian J , Huang B , Shan D , Shi M , Fang C , Yue Y , Li F , Li D , Wei S , Han B , Jiang C , Yin Y , Xia T , Zhang Z , Bennetzen JL , Zhao S , Wan X
Ref : Proc Natl Acad Sci U S A , 115 :E4151 , 2018
Abstract : Tea, one of the world's most important beverage crops, provides numerous secondary metabolites that account for its rich taste and health benefits. Here we present a high-quality sequence of the genome of tea, Camellia sinensis var. sinensis (CSS), using both Illumina and PacBio sequencing technologies. At least 64% of the 3.1-Gb genome assembly consists of repetitive sequences, and the rest yields 33,932 high-confidence predictions of encoded proteins. Divergence between two major lineages, CSS and Camellia sinensis var. assamica (CSA), is calculated to approximately 0.38 to 1.54 million years ago (Mya). Analysis of genic collinearity reveals that the tea genome is the product of two rounds of whole-genome duplications (WGDs) that occurred approximately 30 to 40 and approximately 90 to 100 Mya. We provide evidence that these WGD events, and subsequent paralogous duplications, had major impacts on the copy numbers of secondary metabolite genes, particularly genes critical to producing three key quality compounds: catechins, theanine, and caffeine. Analyses of transcriptome and phytochemistry data show that amplification and transcriptional divergence of genes encoding a large acyltransferase family and leucoanthocyanidin reductases are associated with the characteristic young leaf accumulation of monomeric galloylated catechins in tea, while functional divergence of a single member of the glutamine synthetase gene family yielded theanine synthetase. This genome sequence will facilitate understanding of tea genome evolution and tea metabolite pathways, and will promote germplasm utilization for breeding improved tea varieties.
ESTHER : Wei_2018_Proc.Natl.Acad.Sci.U.S.A_115_E4151
PubMedSearch : Wei_2018_Proc.Natl.Acad.Sci.U.S.A_115_E4151
PubMedID: 29678829
Gene_locus related to this paper: camsi-a0a4s4dr18 , camsi-a0a4s4etg9 , camsi-a0a4s4e3j5 , camsi-a0a4s4d2s5 , camsi-a0a4s4duc4 , camsi-a0a4v3wr80 , camsi-a0a4v3wpu4

Title : A comprehensive draft genome sequence for lupin (Lupinus angustifolius), an emerging health food: insights into plant-microbe interactions and legume evolution - Hane_2017_Plant.Biotechnol.J_15_318
Author(s) : Hane JK , Ming Y , Kamphuis LG , Nelson MN , Garg G , Atkins CA , Bayer PE , Bravo A , Bringans S , Cannon S , Edwards D , Foley R , Gao LL , Harrison MJ , Huang W , Hurgobin B , Li S , Liu CW , McGrath A , Morahan G , Murray J , Weller J , Jian J , Singh KB
Ref : Plant Biotechnol J , 15 :318 , 2017
Abstract : Lupins are important grain legume crops that form a critical part of sustainable farming systems, reducing fertilizer use and providing disease breaks. It has a basal phylogenetic position relative to other crop and model legumes and a high speciation rate. Narrow-leafed lupin (NLL; Lupinus angustifolius L.) is gaining popularity as a health food, which is high in protein and dietary fibre but low in starch and gluten-free. We report the draft genome assembly (609 Mb) of NLL cultivar Tanjil, which has captured >98% of the gene content, sequences of additional lines and a dense genetic map. Lupins are unique among legumes and differ from most other land plants in that they do not form mycorrhizal associations. Remarkably, we find that NLL has lost all mycorrhiza-specific genes, but has retained genes commonly required for mycorrhization and nodulation. In addition, the genome also provided candidate genes for key disease resistance and domestication traits. We also find evidence of a whole-genome triplication at around 25 million years ago in the genistoid lineage leading to Lupinus. Our results will support detailed studies of legume evolution and accelerate lupin breeding programmes.
ESTHER : Hane_2017_Plant.Biotechnol.J_15_318
PubMedSearch : Hane_2017_Plant.Biotechnol.J_15_318
PubMedID: 27557478
Gene_locus related to this paper: lupan-a0a1j7h2u5 , lupan-a0a4p1r201 , lupan-a0a4p1rve4 , lupan-a0a1j7inr2 , lupan-a0a4p1rbl4 , lupan-a0a1j7ifk4 , lupan-a0a4p1rs77 , lupan-a0a1j7h5s4

Title : The pomegranate (Punica granatum L.) genome and the genomics of punicalagin biosynthesis - Qin_2017_Plant.J_91_1108
Author(s) : Qin G , Xu C , Ming R , Tang H , Guyot R , Kramer EM , Hu Y , Yi X , Qi Y , Xu X , Gao Z , Pan H , Jian J , Tian Y , Yue Z , Xu Y
Ref : Plant J , 91 :1108 , 2017
Abstract : Pomegranate (Punica granatum L.) is a perennial fruit crop grown since ancient times that has been planted worldwide and is known for its functional metabolites, particularly punicalagins. We have sequenced and assembled the pomegranate genome with 328 Mb anchored into nine pseudo-chromosomes and annotated 29 229 gene models. A Myrtales lineage-specific whole-genome duplication event was detected that occurred in the common ancestor before the divergence of pomegranate and Eucalyptus. Repetitive sequences accounted for 46.1% of the assembled genome. We found that the integument development gene INNER NO OUTER (INO) was under positive selection and potentially contributed to the development of the fleshy outer layer of the seed coat, an edible part of pomegranate fruit. The genes encoding the enzymes for synthesis and degradation of lignin, hemicelluloses and cellulose were also differentially expressed between soft- and hard-seeded varieties, reflecting differences in their accumulation in cultivars differing in seed hardness. Candidate genes for punicalagin biosynthesis were identified and their expression patterns indicated that gallic acid synthesis in tissues could follow different biochemical pathways. The genome sequence of pomegranate provides a valuable resource for the dissection of many biological and biochemical traits and also provides important insights for the acceleration of breeding. Elucidation of the biochemical pathway(s) involved in punicalagin biosynthesis could assist breeding efforts to increase production of this bioactive compound.
ESTHER : Qin_2017_Plant.J_91_1108
PubMedSearch : Qin_2017_Plant.J_91_1108
PubMedID: 28654223
Gene_locus related to this paper: prupe-a0a251r634 , pungr-a0a218xv87 , pungr-a0a218xi98 , pungr-a0a218wma5 , pungr-a0a218w0a8 , pungr-a0a218w138 , pungr-a0a218w7t6 , pungr-a0a218weu3 , pungr-a0a218xzu6

Title : Prediction and evaluation of the lipase inhibitory activities of tea polyphenols with 3D-QSAR models - Li_2016_Sci.Rep_6_34387
Author(s) : Li YF , Chang YQ , Deng J , Li WX , Jian J , Gao JS , Wan X , Gao H , Kurihara H , Sun PH , He RR
Ref : Sci Rep , 6 :34387 , 2016
Abstract : The extraordinary hypolipidemic effects of polyphenolic compounds from tea have been confirmed in our previous study. To gain compounds with more potent activities, using the conformations of the most active compound revealed by molecular docking, a 3D-QSAR pancreatic lipase inhibitor model with good predictive ability was established and validated by CoMFA and CoMISA methods. With good statistical significance in CoMFA (r2cv = 0.622, r2 = 0.956, F = 261.463, SEE = 0.096) and CoMISA (r2cv = 0.631, r2 = 0.932, F = 75.408, SEE = 0.212) model, we summarized the structure-activity relationship between polyphenolic compounds and pancreatic lipase inhibitory activities and find the bulky substituents in R2, R4 and R5, hydrophilic substituents in R1 and electron withdrawing groups in R2 are the key factors to enhance the lipase inhibitory activities. Under the guidance of the 3D-QSAR results, (2R,3R,2'R,3'R)-desgalloyloolongtheanin-3,3'-O-digallate (DOTD), a potent lipase inhibitor with an IC50 of 0.08 mug/ml, was obtained from EGCG oxidative polymerization catalyzed by crude polyphenol oxidase. Furthermore, DOTD was found to inhibit lipid absorption in olive oil-loaded rats, which was related with inhibiting the activities of lipase in the intestinal mucosa and contents.
ESTHER : Li_2016_Sci.Rep_6_34387
PubMedSearch : Li_2016_Sci.Rep_6_34387
PubMedID: 27694956

Title : Genomic study of polyhydroxyalkanoates producing Aeromonas hydrophila 4AK4 - Gao_2013_Appl.Microbiol.Biotechnol_97_9099
Author(s) : Gao X , Jian J , Li WJ , Yang YC , Shen XW , Sun ZR , Wu Q , Chen GQ
Ref : Applied Microbiology & Biotechnology , 97 :9099 , 2013
Abstract : The complete genome of Gram-negative Aeromonas hydrophila 4AK4 that has been used for industrial production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) was sequenced and annotated. Its chromosome is 4,527,993 bp in size encoding 4,272 genes, including 28 rRNA genes and 104 tRNA genes. Comparative analysis indicated that genome of A. hydrophila 4AK4 was similar to that of the A. hydrophila ATCC 7966(T), an intensively studied aeromonad for its pathogenicity related to its genomic information. Genes possibly coming from other species or even other genus were identified in A. hydrophila 4AK4. A large number of putative virulent genes were predicted. However, a cytotonic enterotoxin (Ast) is absent in A. hydrophila 4AK4, allowing the industrial strain to be different from other A. hydrophila strains, indicating possible reduced virulence of strain 4AK4, which is very important for industrial fermentation. Genes involved in polyhydroxyalkanoate (PHA) metabolism were predicted and analyzed. The resulting genomic information is useful for improved production of PHA via metabolic engineering of A. hydrophila 4AK4.
ESTHER : Gao_2013_Appl.Microbiol.Biotechnol_97_9099
PubMedSearch : Gao_2013_Appl.Microbiol.Biotechnol_97_9099
PubMedID: 24000047
Gene_locus related to this paper: aerme-a0a023rmd0 , aervb-f4dgq1

Title : Draft genome of the wheat A-genome progenitor Triticum urartu - Ling_2013_Nature_496_87
Author(s) : Ling HQ , Zhao S , Liu D , Wang J , Sun H , Zhang C , Fan H , Li D , Dong L , Tao Y , Gao C , Wu H , Li Y , Cui Y , Guo X , Zheng S , Wang B , Yu K , Liang Q , Yang W , Lou X , Chen J , Feng M , Jian J , Zhang X , Luo G , Jiang Y , Liu J , Wang Z , Sha Y , Zhang B , Tang D , Shen Q , Xue P , Zou S , Wang X , Liu X , Wang F , Yang Y , An X , Dong Z , Zhang K , Luo MC , Dvorak J , Tong Y , Yang H , Li Z , Wang D , Zhang A
Ref : Nature , 496 :87 , 2013
Abstract : Bread wheat (Triticum aestivum, AABBDD) is one of the most widely cultivated and consumed food crops in the world. However, the complex polyploid nature of its genome makes genetic and functional analyses extremely challenging. The A genome, as a basic genome of bread wheat and other polyploid wheats, for example, T. turgidum (AABB), T. timopheevii (AAGG) and T. zhukovskyi (AAGGA(m)A(m)), is central to wheat evolution, domestication and genetic improvement. The progenitor species of the A genome is the diploid wild einkorn wheat T. urartu, which resembles cultivated wheat more extensively than do Aegilops speltoides (the ancestor of the B genome) and Ae. tauschii (the donor of the D genome), especially in the morphology and development of spike and seed. Here we present the generation, assembly and analysis of a whole-genome shotgun draft sequence of the T. urartu genome. We identified protein-coding gene models, performed genome structure analyses and assessed its utility for analysing agronomically important genes and for developing molecular markers. Our T. urartu genome assembly provides a diploid reference for analysis of polyploid wheat genomes and is a valuable resource for the genetic improvement of wheat.
ESTHER : Ling_2013_Nature_496_87
PubMedSearch : Ling_2013_Nature_496_87
PubMedID: 23535596
Gene_locus related to this paper: triua-m8a764 , triua-m8ag96 , triua-m7zp69 , wheat-w5d1z6 , wheat-w5d232 , wheat-w5bnf5 , triua-t1nm05 , wheat-w5cae4 , triua-m7ytf7 , wheat-w5f1j8 , triua-m8ad49 , wheat-a0a077s1q2 , wheat-a0a3b6c2m6 , triua-m7zi26 , wheat-a0a3b6at77 , wheat-a0a3b6atp7

Title : Molecular cloning and functional analysis of two polyhydroxyalkanoate synthases from two strains of Aeromonas hydrophila spp - Lu_2005_FEMS.Microbiol.Lett_243_149
Author(s) : Lu X , Zhang W , Jian J , Wu Q , Chen GQ
Ref : FEMS Microbiology Letters , 243 :149 , 2005
Abstract : Polyhydroxyalkanoate (PHA) synthase genes (phaC) were cloned from two Aeromonas hydrophila strains named WQ and 4AK5, respectively. Both strains are able to produce PHBHHx copolyesters consisting of 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HHx). Sequence analysis showed that there was only 2 bp difference between these two PHA synthase genes, corresponding to two-amino acid difference at positions of 437 and 458 of the two synthases. PHA productivity and its monomer content produced by A. hydrophila WQ and A. hydrophila 4AK5 were quite different. A. hydrophila WQ accumulated 33% PHBHHx of its cell dry weight (CDW) with 5 mol% 3HHx in the copolyester when cultured in lauric acid for 48 h. Yet A. hydrophila 4AK5 was able to produce 43% PHBHHx of the CDW with 14 mol% 3HHx under the same condition. Hetero-expression of PHA synthase genes of A. hydrophila WQ and A. hydrophila 4AK5, respectively, in Escherichia coli XL1-Blue led to PHBHHx accumulation of 24% and 39% of the CDW and the 3HHx content in PHBHHx were 6 and 15 mol%, respectively. This indicated that the function of these two PHA synthases were different due to these two different residues at positions of 437 and 458. Site specific mutation was carried out to change these two amino acid residues. Results showed that the changes on either of the two amino acids negatively affected the PHA productivity.
ESTHER : Lu_2005_FEMS.Microbiol.Lett_243_149
PubMedSearch : Lu_2005_FEMS.Microbiol.Lett_243_149
PubMedID: 15668013
Gene_locus related to this paper: aerhy-PHAC