Hane JK

References (10)

Title : Genomic and Genetic Insights Into a Cosmopolitan Fungus, Paecilomyces variotii (Eurotiales) - Urquhart_2018_Front.Microbiol_9_3058
Author(s) : Urquhart AS , Mondo SJ , Makela MR , Hane JK , Wiebenga A , He G , Mihaltcheva S , Pangilinan J , Lipzen A , Barry K , de Vries RP , Grigoriev IV , Idnurm A
Ref : Front Microbiol , 9 :3058 , 2018
Abstract : Species in the genus Paecilomyces, a member of the fungal order Eurotiales, are ubiquitous in nature and impact a variety of human endeavors. Here, the biology of one common species, Paecilomyces variotii, was explored using genomics and functional genetics. Sequencing the genome of two isolates revealed key genome and gene features in this species. A striking feature of the genome was the two-part nature, featuring large stretches of DNA with normal GC content separated by AT-rich regions, a hallmark of many plant-pathogenic fungal genomes. These AT-rich regions appeared to have been mutated by repeat-induced point (RIP) mutations. We developed methods for genetic transformation of P. variotii, including forward and reverse genetics as well as crossing techniques. Using transformation and crossing, RIP activity was identified, demonstrating for the first time that RIP is an active process within the order Eurotiales. A consequence of RIP is likely reflected by a reduction in numbers of genes within gene families, such as in cell wall degradation, and reflected by growth limitations on P. variotii on diverse carbon sources. Furthermore, using these transformation tools we characterized a conserved protein containing a domain of unknown function (DUF1212) and discovered it is involved in pigmentation.
ESTHER : Urquhart_2018_Front.Microbiol_9_3058
PubMedSearch : Urquhart_2018_Front.Microbiol_9_3058
PubMedID: 30619145
Gene_locus related to this paper: byssp-a0a443i770 , byssp-a0a443i5x3 , byssp-vdta1

Title : Comparative genomics of the wheat fungal pathogen Pyrenophora tritici-repentis reveals chromosomal variations and genome plasticity - Moolhuijzen_2018_BMC.Genomics_19_279
Author(s) : Moolhuijzen P , See PT , Hane JK , Shi G , Liu Z , Oliver RP , Moffat CS
Ref : BMC Genomics , 19 :279 , 2018
Abstract : BACKGROUND: Pyrenophora tritici-repentis (Ptr) is a necrotrophic fungal pathogen that causes the major wheat disease, tan spot. We set out to provide essential genomics-based resources in order to better understand the pathogenicity mechanisms of this important pathogen. RESULTS: Here, we present eight new Ptr isolate genomes, assembled and annotated; representing races 1, 2 and 5, and a new race. We report a high quality Ptr reference genome, sequenced by PacBio technology with Illumina paired-end data support and optical mapping. An estimated 98% of the genome coverage was mapped to 10 chromosomal groups, using a two-enzyme hybrid approach. The final reference genome was 40.9 Mb and contained a total of 13,797 annotated genes, supported by transcriptomic and proteogenomics data sets. CONCLUSIONS: Whole genome comparative analysis revealed major chromosomal segmental rearrangements and fusions, highlighting intraspecific genome plasticity in this species. Furthermore, the Ptr race classification was not supported at the whole genome level, as phylogenetic analysis did not cluster the ToxA producing isolates. This expansion of available Ptr genomics resources will directly facilitate research aimed at controlling tan spot disease.
ESTHER : Moolhuijzen_2018_BMC.Genomics_19_279
PubMedSearch : Moolhuijzen_2018_BMC.Genomics_19_279
PubMedID: 29685100
Gene_locus related to this paper: 9pleo-a0a2w1h6j9 , pyrtr-b2vzr5 , 9pleo-a0a2w1duy4

Title : A comprehensive draft genome sequence for lupin (Lupinus angustifolius), an emerging health food: insights into plant-microbe interactions and legume evolution - Hane_2017_Plant.Biotechnol.J_15_318
Author(s) : Hane JK , Ming Y , Kamphuis LG , Nelson MN , Garg G , Atkins CA , Bayer PE , Bravo A , Bringans S , Cannon S , Edwards D , Foley R , Gao LL , Harrison MJ , Huang W , Hurgobin B , Li S , Liu CW , McGrath A , Morahan G , Murray J , Weller J , Jian J , Singh KB
Ref : Plant Biotechnol J , 15 :318 , 2017
Abstract : Lupins are important grain legume crops that form a critical part of sustainable farming systems, reducing fertilizer use and providing disease breaks. It has a basal phylogenetic position relative to other crop and model legumes and a high speciation rate. Narrow-leafed lupin (NLL; Lupinus angustifolius L.) is gaining popularity as a health food, which is high in protein and dietary fibre but low in starch and gluten-free. We report the draft genome assembly (609 Mb) of NLL cultivar Tanjil, which has captured >98% of the gene content, sequences of additional lines and a dense genetic map. Lupins are unique among legumes and differ from most other land plants in that they do not form mycorrhizal associations. Remarkably, we find that NLL has lost all mycorrhiza-specific genes, but has retained genes commonly required for mycorrhization and nodulation. In addition, the genome also provided candidate genes for key disease resistance and domestication traits. We also find evidence of a whole-genome triplication at around 25 million years ago in the genistoid lineage leading to Lupinus. Our results will support detailed studies of legume evolution and accelerate lupin breeding programmes.
ESTHER : Hane_2017_Plant.Biotechnol.J_15_318
PubMedSearch : Hane_2017_Plant.Biotechnol.J_15_318
PubMedID: 27557478
Gene_locus related to this paper: lupan-a0a1j7h2u5 , lupan-a0a4p1r201 , lupan-a0a4p1rve4 , lupan-a0a1j7inr2 , lupan-a0a4p1rbl4 , lupan-a0a1j7ifk4 , lupan-a0a4p1rs77 , lupan-a0a1j7h5s4

Title : Genome sequencing and comparative genomics of the broad host-range pathogen Rhizoctonia solani AG8 - Hane_2014_PLoS.Genet_10_e1004281
Author(s) : Hane JK , Anderson JP , Williams AH , Sperschneider J , Singh KB
Ref : PLoS Genet , 10 :e1004281 , 2014
Abstract : Rhizoctonia solani is a soil-borne basidiomycete fungus with a necrotrophic lifestyle which is classified into fourteen reproductively incompatible anastomosis groups (AGs). One of these, AG8, is a devastating pathogen causing bare patch of cereals, brassicas and legumes. R. solani is a multinucleate heterokaryon containing significant heterozygosity within a single cell. This complexity posed significant challenges for the assembly of its genome. We present a high quality genome assembly of R. solani AG8 and a manually curated set of 13,964 genes supported by RNA-seq. The AG8 genome assembly used novel methods to produce a haploid representation of its heterokaryotic state. The whole-genomes of AG8, the rice pathogen AG1-IA and the potato pathogen AG3 were observed to be syntenic and co-linear. Genes and functions putatively relevant to pathogenicity were highlighted by comparing AG8 to known pathogenicity genes, orthology databases spanning 197 phytopathogenic taxa and AG1-IA. We also observed SNP-level "hypermutation" of CpG dinucleotides to TpG between AG8 nuclei, with similarities to repeat-induced point mutation (RIP). Interestingly, gene-coding regions were widely affected along with repetitive DNA, which has not been previously observed for RIP in mononuclear fungi of the Pezizomycotina. The rate of heterozygous SNP mutations within this single isolate of AG8 was observed to be higher than SNP mutation rates observed across populations of most fungal species compared. Comparative analyses were combined to predict biological processes relevant to AG8 and 308 proteins with effector-like characteristics, forming a valuable resource for further study of this pathosystem. Predicted effector-like proteins had elevated levels of non-synonymous point mutations relative to synonymous mutations (dN/dS), suggesting that they may be under diversifying selection pressures. In addition, the distant relationship to sequenced necrotrophs of the Ascomycota suggests the R. solani genome sequence may prove to be a useful resource in future comparative analysis of plant pathogens.
ESTHER : Hane_2014_PLoS.Genet_10_e1004281
PubMedSearch : Hane_2014_PLoS.Genet_10_e1004281
PubMedID: 24810276
Gene_locus related to this paper: 9homo-a0a066vzn3 , 9homo-a0a066v6w3 , 9homo-a0a066vd48 , 9homo-a0a066wb64 , 9homo-a0a066w438 , 9homo-a0a066vyx1

Title : The genome sequence of the biocontrol fungus Metarhizium anisopliae and comparative genomics of Metarhizium species - Pattemore_2014_BMC.Genomics_15_660
Author(s) : Pattemore JA , Hane JK , Williams AH , Wilson BA , Stodart BJ , Ash GJ
Ref : BMC Genomics , 15 :660 , 2014
Abstract : BACKGROUND: Metarhizium anisopliae is an important fungal biocontrol agent of insect pests of agricultural crops. Genomics can aid the successful commercialization of biopesticides by identification of key genes differentiating closely related species, selection of virulent microbial isolates which are amenable to industrial scale production and formulation and through the reduction of phenotypic variability. The genome of Metarhizium isolate ARSEF23 was recently published as a model for M. anisopliae, however phylogenetic analysis has since re-classified this isolate as M. robertsii. We present a new annotated genome sequence of M. anisopliae (isolate Ma69) and whole genome comparison to M. robertsii (ARSEF23) and M. acridum (CQMa 102).
RESULTS: Whole genome analysis of M. anisopliae indicates significant macrosynteny with M. robertsii but with some large genomic inversions. In comparison to M. acridum, the genome of M. anisopliae shares lower sequence homology. While alignments overall are co-linear, the genome of M. acridum is not contiguous enough to conclusively observe macrosynteny. Mating type gene analysis revealed both MAT1-1 and MAT1-2 genes present in M. anisopliae suggesting putative homothallism, despite having no known teleomorph, in contrast with the putatively heterothallic M. acridum isolate CQMa 102 (MAT1-2) and M. robertsii isolate ARSEF23 (altered MAT1-1). Repetitive DNA and RIP analysis revealed M. acridum to have twice the repetitive content of the other two species and M. anisopliae to be five times more RIP affected than M. robertsii. We also present an initial bioinformatic survey of candidate pathogenicity genes in M. anisopliae.
CONCLUSIONS: The annotated genome of M. anisopliae is an important resource for the identification of virulence genes specific to M. anisopliae and development of species- and strain- specific assays. New insight into the possibility of homothallism and RIP affectedness has important implications for the development of M. anisopliae as a biopesticide as it may indicate the potential for greater inherent diversity in this species than the other species. This could present opportunities to select isolates with unique combinations of pathogenicity factors, or it may point to instability in the species, a negative attribute in a biopesticide.
ESTHER : Pattemore_2014_BMC.Genomics_15_660
PubMedSearch : Pattemore_2014_BMC.Genomics_15_660
PubMedID: 25102932
Gene_locus related to this paper: metan-a0a086npb7 , metan-a0a0d9nj46 , metan-a0a0d9nzf0 , metan-a0a0d9p521 , metan-a0a0d9pev7 , metan-a0a0d9nta1

Title : Comparative genomics of a plant-pathogenic fungus, Pyrenophora tritici-repentis, reveals transduplication and the impact of repeat elements on pathogenicity and population divergence - Manning_2013_G3.(Bethesda)_3_41
Author(s) : Manning VA , Pandelova I , Dhillon B , Wilhelm LJ , Goodwin SB , Berlin AM , Figueroa M , Freitag M , Hane JK , Henrissat B , Holman WH , Kodira CD , Martin J , Oliver RP , Robbertse B , Schackwitz W , Schwartz DC , Spatafora JW , Turgeon BG , Yandava C , Young S , Zhou S , Zeng Q , Grigoriev IV , Ma LJ , Ciuffetti LM
Ref : G3 (Bethesda) , 3 :41 , 2013
Abstract : Pyrenophora tritici-repentis is a necrotrophic fungus causal to the disease tan spot of wheat, whose contribution to crop loss has increased significantly during the last few decades. Pathogenicity by this fungus is attributed to the production of host-selective toxins (HST), which are recognized by their host in a genotype-specific manner. To better understand the mechanisms that have led to the increase in disease incidence related to this pathogen, we sequenced the genomes of three P. tritici-repentis isolates. A pathogenic isolate that produces two known HSTs was used to assemble a reference nuclear genome of approximately 40 Mb composed of 11 chromosomes that encode 12,141 predicted genes. Comparison of the reference genome with those of a pathogenic isolate that produces a third HST, and a nonpathogenic isolate, showed the nonpathogen genome to be more diverged than those of the two pathogens. Examination of gene-coding regions has provided candidate pathogen-specific proteins and revealed gene families that may play a role in a necrotrophic lifestyle. Analysis of transposable elements suggests that their presence in the genome of pathogenic isolates contributes to the creation of novel genes, effector diversification, possible horizontal gene transfer events, identified copy number variation, and the first example of transduplication by DNA transposable elements in fungi. Overall, comparative analysis of these genomes provides evidence that pathogenicity in this species arose through an influx of transposable elements, which created a genetically flexible landscape that can easily respond to environmental changes.
ESTHER : Manning_2013_G3.(Bethesda)_3_41
PubMedSearch : Manning_2013_G3.(Bethesda)_3_41
PubMedID: 23316438
Gene_locus related to this paper: pyrtr-b2vxe8 , pyrtr-b2vvm1 , pyrtr-b2vzr5 , pyrtr-b2vu22 , pyrtr-kex1

Title : Finished genome of the fungal wheat pathogen Mycosphaerella graminicola reveals dispensome structure, chromosome plasticity, and stealth pathogenesis - Goodwin_2011_PLoS.Genet_7_e1002070
Author(s) : Goodwin SB , M'Barek S B , Dhillon B , Wittenberg AH , Crane CF , Hane JK , Foster AJ , Van der Lee TA , Grimwood J , Aerts A , Antoniw J , Bailey A , Bluhm B , Bowler J , Bristow J , van der Burgt A , Canto-Canche B , Churchill AC , Conde-Ferraez L , Cools HJ , Coutinho PM , Csukai M , Dehal P , De Wit P , Donzelli B , van de Geest HC , van Ham RC , Hammond-Kosack KE , Henrissat B , Kilian A , Kobayashi AK , Koopmann E , Kourmpetis Y , Kuzniar A , Lindquist E , Lombard V , Maliepaard C , Martins N , Mehrabi R , Nap JP , Ponomarenko A , Rudd JJ , Salamov A , Schmutz J , Schouten HJ , Shapiro H , Stergiopoulos I , Torriani SF , Tu H , de Vries RP , Waalwijk C , Ware SB , Wiebenga A , Zwiers LH , Oliver RP , Grigoriev IV , Kema GH
Ref : PLoS Genet , 7 :e1002070 , 2011
Abstract : The plant-pathogenic fungus Mycosphaerella graminicola (asexual stage: Septoria tritici) causes septoria tritici blotch, a disease that greatly reduces the yield and quality of wheat. This disease is economically important in most wheat-growing areas worldwide and threatens global food production. Control of the disease has been hampered by a limited understanding of the genetic and biochemical bases of pathogenicity, including mechanisms of infection and of resistance in the host. Unlike most other plant pathogens, M. graminicola has a long latent period during which it evades host defenses. Although this type of stealth pathogenicity occurs commonly in Mycosphaerella and other Dothideomycetes, the largest class of plant-pathogenic fungi, its genetic basis is not known. To address this problem, the genome of M. graminicola was sequenced completely. The finished genome contains 21 chromosomes, eight of which could be lost with no visible effect on the fungus and thus are dispensable. This eight-chromosome dispensome is dynamic in field and progeny isolates, is different from the core genome in gene and repeat content, and appears to have originated by ancient horizontal transfer from an unknown donor. Synteny plots of the M. graminicola chromosomes versus those of the only other sequenced Dothideomycete, Stagonospora nodorum, revealed conservation of gene content but not order or orientation, suggesting a high rate of intra-chromosomal rearrangement in one or both species. This observed "mesosynteny" is very different from synteny seen between other organisms. A surprising feature of the M. graminicola genome compared to other sequenced plant pathogens was that it contained very few genes for enzymes that break down plant cell walls, which was more similar to endophytes than to pathogens. The stealth pathogenesis of M. graminicola probably involves degradation of proteins rather than carbohydrates to evade host defenses during the biotrophic stage of infection and may have evolved from endophytic ancestors.
ESTHER : Goodwin_2011_PLoS.Genet_7_e1002070
PubMedSearch : Goodwin_2011_PLoS.Genet_7_e1002070
PubMedID: 21695235
Gene_locus related to this paper: zymti-f9wzw8 , zymti-f9x2y6 , zymti-f9x423 , zymti-f9x813 , zymti-f9xa54 , zymti-f9xb42 , zymti-f9xbu5 , zymti-f9xcr9 , zymti-f9xdr7 , zymti-f9xer1 , zymti-f9xez8 , zymti-f9xfz9 , zymti-f9xh29 , zymti-f9xhe7 , zymti-f9xhr4 , zymti-f9xk09 , zymti-f9xns5 , zymti-f9xiu1 , zymti-f9xng3 , zymti-f9x4f2 , zymti-f9x4s7 , zymti-f9xdm8 , zymti-f9wwy9 , zymti-f9xkf2 , zymti-f9xlt3 , zymti-f9x0i3 , zymti-f9wwa6 , zymti-f9wyk7 , zymti-f9x3z1 , zymti-f9xf16 , zymtr-a0a1x7rhi5 , zymti-f9xfj3 , zymti-pks1

Title : Effector diversification within compartments of the Leptosphaeria maculans genome affected by Repeat-Induced Point mutations - Rouxel_2011_Nat.Commun_2_202
Author(s) : Rouxel T , Grandaubert J , Hane JK , Hoede C , van de Wouw AP , Couloux A , Dominguez V , Anthouard V , Bally P , Bourras S , Cozijnsen AJ , Ciuffetti LM , Degrave A , Dilmaghani A , Duret L , Fudal I , Goodwin SB , Gout L , Glaser N , Linglin J , Kema GH , Lapalu N , Lawrence CB , May K , Meyer M , Ollivier B , Poulain J , Schoch CL , Simon A , Spatafora JW , Stachowiak A , Turgeon BG , Tyler BM , Vincent D , Weissenbach J , Amselem J , Quesneville H , Oliver RP , Wincker P , Balesdent MH , Howlett BJ
Ref : Nat Commun , 2 :202 , 2011
Abstract : Fungi are of primary ecological, biotechnological and economic importance. Many fundamental biological processes that are shared by animals and fungi are studied in fungi due to their experimental tractability. Many fungi are pathogens or mutualists and are model systems to analyse effector genes and their mechanisms of diversification. In this study, we report the genome sequence of the phytopathogenic ascomycete Leptosphaeria maculans and characterize its repertoire of protein effectors. The L. maculans genome has an unusual bipartite structure with alternating distinct guanine and cytosine-equilibrated and adenine and thymine (AT)-rich blocks of homogenous nucleotide composition. The AT-rich blocks comprise one-third of the genome and contain effector genes and families of transposable elements, both of which are affected by repeat-induced point mutation, a fungal-specific genome defence mechanism. This genomic environment for effectors promotes rapid sequence diversification and underpins the evolutionary potential of the fungus to adapt rapidly to novel host-derived constraints.
ESTHER : Rouxel_2011_Nat.Commun_2_202
PubMedSearch : Rouxel_2011_Nat.Commun_2_202
PubMedID: 21326234
Gene_locus related to this paper: lepmc-q6q891 , lepmj-e4zh04 , lepmj-e4ziv6 , lepmj-e4zju4 , lepmj-e4zqu4 , lepmj-e4zvh3 , lepmj-e4zvl4 , lepmj-e4zx66 , lepmj-e5a0i2 , lepmj-e5a510 , lepmj-e5aau5 , lepmj-e5acd1 , lepmj-e5a4g5 , lepmj-e4zhg2 , lepmj-e4zuw5 , lepmj-e5a2e0 , lepmj-e4zpv2 , lepmj-e4zxp4 , lepmj-e4zpy5 , lepmj-e5ae17 , lepmj-e4ziv2 , lepmj-e5a374 , lepmj-e5ab81 , lepmj-e4zgy1 , lepmj-e4zg43 , lepmj-kex1 , lepmj-cbpya

Title : A first genome assembly of the barley fungal pathogen Pyrenophora teres f. teres - Ellwood_2010_Genome.Biol_11_R109
Author(s) : Ellwood SR , Liu Z , Syme RA , Lai Z , Hane JK , Keiper F , Moffat CS , Oliver RP , Friesen TL
Ref : Genome Biol , 11 :R109 , 2010
Abstract : BACKGROUND: Pyrenophora teres f. teres is a necrotrophic fungal pathogen and the cause of one of barley's most important diseases, net form of net blotch. Here we report the first genome assembly for this species based solely on short Solexa sequencing reads of isolate 0-1. The assembly was validated by comparison to BAC sequences, ESTs, orthologous genes and by PCR, and complemented by cytogenetic karyotyping and the first genome-wide genetic map for P. teres f. teres.
RESULTS: The total assembly was 41.95 Mbp and contains 11,799 gene models of 50 amino acids or more. Comparison against two sequenced BACs showed that complex regions with a high GC content assembled effectively. Electrophoretic karyotyping showed distinct chromosomal polymorphisms between isolates 0-1 and 15A, and cytological karyotyping confirmed the presence of at least nine chromosomes. The genetic map spans 2477.7 cM and is composed of 243 markers in 25 linkage groups, and incorporates simple sequence repeat markers developed from the assembly. Among predicted genes, non-ribosomal peptide synthetases and efflux pumps in particular appear to have undergone a P. teres f. teres-specific expansion of non-orthologous gene families.
CONCLUSIONS: This study demonstrates that paired-end Solexa sequencing can successfully capture coding regions of a filamentous fungal genome. The assembly contains a plethora of predicted genes that have been implicated in a necrotrophic lifestyle and pathogenicity and presents a significant resource for examining the bases for P. teres f. teres pathogenicity.
ESTHER : Ellwood_2010_Genome.Biol_11_R109
PubMedSearch : Ellwood_2010_Genome.Biol_11_R109
PubMedID: 21067574
Gene_locus related to this paper: pyrtr-b2vv71 , pyrtr-b2w1y6 , pyrtr-b2w948 , pyrtr-b2wbz8 , pyrtr-b2wcq8 , pyrtr-b2wgg5 , pyrtr-dapb , pyrtt-e3rcw4 , pyrtt-e3rfb1 , pyrtt-e3rfu0 , pyrtt-e3rgg7 , pyrtt-e3rh98 , pyrtt-e3ril1 , pyrtt-e3rjq5 , pyrtt-e3rkg6 , pyrtt-e3rkr9 , pyrtt-e3rl87 , pyrtt-e3rp29 , pyrtt-e3rpe8 , pyrtt-e3rqg8 , pyrtt-e3rrt3 , pyrtt-e3rs40 , pyrtt-e3rth6 , pyrtt-e3rtt7 , pyrtt-e3ruq0 , pyrtt-e3ruw6 , pyrtt-e3rvm2 , pyrtt-e3rwp7 , pyrtt-e3rxf7 , pyrtt-e3s2m6 , pyrtt-e3s5q8 , pyrtt-e3s8r6 , pyrtt-e3s292 , pyrtt-e3s479 , pyrtt-e3s701 , pyrtt-e3s716 , pyrtt-e3s922 , pyrtt-e3sa06 , pyrtt-e3ru14 , pyrtt-e3rg15 , pyrtt-e3rxy1 , pyrtr-b2vyg1 , pyrtt-e3rnt0 , pyrtt-e3rwh1 , pyrtt-e3rtw2 , pyrtt-e3s3k4 , pyrtt-e3rs45 , pyrtr-b2w571 , pyrtt-e3s9z0 , pyrtt-e3rxj8 , pyrtt-e3s3p6 , pyrtt-e3rqx0 , pyrtt-e3rpq8 , pyrtt-e3rx11 , pyrtr-b2vzr5 , pyrtt-e3s4g0 , pyrtt-e3rqw7 , pyrtt-e3rgu8

Title : Dothideomycete plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum - Hane_2007_Plant.Cell_19_3347
Author(s) : Hane JK , Lowe RG , Solomon PS , Tan KC , Schoch CL , Spatafora JW , Crous PW , Kodira C , Birren BW , Galagan JE , Torriani SF , McDonald BA , Oliver RP
Ref : Plant Cell , 19 :3347 , 2007
Abstract : Stagonospora nodorum is a major necrotrophic fungal pathogen of wheat (Triticum aestivum) and a member of the Dothideomycetes, a large fungal taxon that includes many important plant pathogens affecting all major crop plant families. Here, we report the acquisition and initial analysis of a draft genome sequence for this fungus. The assembly comprises 37,164,227 bp of nuclear DNA contained in 107 scaffolds. The circular mitochondrial genome comprises 49,761 bp encoding 46 genes, including four that are intron encoded. The nuclear genome assembly contains 26 classes of repetitive DNA, comprising 4.5% of the genome. Some of the repeats show evidence of repeat-induced point mutations consistent with a frequent sexual cycle. ESTs and gene prediction models support a minimum of 10,762 nuclear genes. Extensive orthology was found between the polyketide synthase family in S. nodorum and Cochliobolus heterostrophus, suggesting an ancient origin and conserved functions for these genes. A striking feature of the gene catalog was the large number of genes predicted to encode secreted proteins; the majority has no meaningful similarity to any other known genes. It is likely that genes for host-specific toxins, in addition to ToxA, will be found among this group. ESTs obtained from axenic mycelium grown on oleate (chosen to mimic early infection) and late-stage lesions sporulating on wheat leaves were obtained. Statistical analysis shows that transcripts encoding proteins involved in protein synthesis and in the production of extracellular proteases, cellulases, and xylanases predominate in the infection library. This suggests that the fungus is dependant on the degradation of wheat macromolecular constituents to provide the carbon skeletons and energy for the synthesis of proteins and other components destined for the developing pycnidiospores.
ESTHER : Hane_2007_Plant.Cell_19_3347
PubMedSearch : Hane_2007_Plant.Cell_19_3347
PubMedID: 18024570
Gene_locus related to this paper: phano-at151 , phano-bst1 , phano-dapb , phano-q0tw42 , phano-q0txi3 , phano-q0tzh6 , phano-q0u0b1 , phano-q0u0e9 , phano-q0u0t5 , phano-q0u1e7 , phano-q0u1v1 , phano-q0u1x4 , phano-q0u2m9 , phano-q0u2y5 , phano-q0u3i1 , phano-q0u5t8 , phano-q0u8z8 , phano-q0u382 , phano-q0u622 , phano-q0u753 , phano-q0uac8 , phano-q0ucn2 , phano-q0udp4 , phano-q0udr2 , phano-q0uem1 , phano-q0uet5 , phano-q0ufs1 , phano-q0ug08 , phano-q0ugf2 , phano-q0uha0 , phano-q0ui22 , phano-q0ukj9 , phano-q0unt6 , phano-q0upq4 , phano-q0uq74 , phano-q0uq76 , phano-q0urz6 , phano-q0uua1 , phano-q0uuc4 , phano-q0uuc6 , phano-q0uup2 , phano-q0uv86 , phano-q0uwb7 , phano-q0uyc1 , phano-q0uyf1 , phano-q0uyi9 , phano-q0v0y6 , phano-q0v1c5 , phano-q0v1s1 , phano-q0v2i6 , phano-q0v4x4 , phano-q0v5x9 , phano-q0v6t6 , phano-q0v6w1 , phano-q0v7e6 , phano-q0v615 , phano-q0uru8 , phano-q0txx8 , phano-q0u4u8 , phano-q0v3l0 , phano-q0ua76 , phano-q0ty51 , phano-q0twk1 , phano-q0v263 , phano-q0uh92 , phano-q0uxx8 , phano-q0v697 , phano-q0ug10 , phano-q0v5u8 , phano-q0u1s8 , phano-q0uy34 , phano-q0twn7 , phano-elca , phano-cbpya , phano-kex1 , phano-q0v778