Rupp O

References (11)

Title : A reference genome of the Chinese hamster based on a hybrid assembly strategy - Rupp_2018_Biotechnol.Bioeng_115_2087
Author(s) : Rupp O , MacDonald ML , Li S , Dhiman H , Polson S , Griep S , Heffner K , Hernandez I , Brinkrolf K , Jadhav V , Samoudi M , Hao H , Kingham B , Goesmann A , Betenbaugh MJ , Lewis NE , Borth N , Lee KH
Ref : Biotechnol Bioeng , 115 :2087 , 2018
Abstract : Accurate and complete genome sequences are essential in biotechnology to facilitate genome-based cell engineering efforts. The current genome assemblies for Cricetulus griseus, the Chinese hamster, are fragmented and replete with gap sequences and misassemblies, consistent with most short-read-based assemblies. Here, we completely resequenced C. griseus using single molecule real time sequencing and merged this with Illumina-based assemblies. This generated a more contiguous and complete genome assembly than either technology alone, reducing the number of scaffolds by >28-fold, with 90% of the sequence in the 122 longest scaffolds. Most genes are now found in single scaffolds, including up- and downstream regulatory elements, enabling improved study of noncoding regions. With >95% of the gap sequence filled, important Chinese hamster ovary cell mutations have been detected in draft assembly gaps. This new assembly will be an invaluable resource for continued basic and pharmaceutical research.
ESTHER : Rupp_2018_Biotechnol.Bioeng_115_2087
PubMedSearch : Rupp_2018_Biotechnol.Bioeng_115_2087
PubMedID: 29704459
Gene_locus related to this paper: crigr-g3h083 , crigr-a0a3l7ib08 , crigr-g3ifk5 , crigr-g3h7k6

Title : The genome of the recently domesticated crop plant sugar beet (Beta vulgaris) - Dohm_2014_Nature_505_546
Author(s) : Dohm JC , Minoche AE , Holtgrawe D , Capella-Gutierrez S , Zakrzewski F , Tafer H , Rupp O , Sorensen TR , Stracke R , Reinhardt R , Goesmann A , Kraft T , Schulz B , Stadler PF , Schmidt T , Gabaldon T , Lehrach H , Weisshaar B , Himmelbauer H
Ref : Nature , 505 :546 , 2014
Abstract : Sugar beet (Beta vulgaris ssp. vulgaris) is an important crop of temperate climates which provides nearly 30% of the world's annual sugar production and is a source for bioethanol and animal feed. The species belongs to the order of Caryophylalles, is diploid with 2n = 18 chromosomes, has an estimated genome size of 714-758 megabases and shares an ancient genome triplication with other eudicot plants. Leafy beets have been cultivated since Roman times, but sugar beet is one of the most recently domesticated crops. It arose in the late eighteenth century when lines accumulating sugar in the storage root were selected from crosses made with chard and fodder beet. Here we present a reference genome sequence for sugar beet as the first non-rosid, non-asterid eudicot genome, advancing comparative genomics and phylogenetic reconstructions. The genome sequence comprises 567 megabases, of which 85% could be assigned to chromosomes. The assembly covers a large proportion of the repetitive sequence content that was estimated to be 63%. We predicted 27,421 protein-coding genes supported by transcript data and annotated them on the basis of sequence homology. Phylogenetic analyses provided evidence for the separation of Caryophyllales before the split of asterids and rosids, and revealed lineage-specific gene family expansions and losses. We sequenced spinach (Spinacia oleracea), another Caryophyllales species, and validated features that separate this clade from rosids and asterids. Intraspecific genomic variation was analysed based on the genome sequences of sea beet (Beta vulgaris ssp. maritima; progenitor of all beet crops) and four additional sugar beet accessions. We identified seven million variant positions in the reference genome, and also large regions of low variability, indicating artificial selection. The sugar beet genome sequence enables the identification of genes affecting agronomically relevant traits, supports molecular breeding and maximizes the plant's potential in energy biotechnology.
ESTHER : Dohm_2014_Nature_505_546
PubMedSearch : Dohm_2014_Nature_505_546
PubMedID: 24352233
Gene_locus related to this paper: betvu-a0a0j8bcg5 , betvu-a0a0j8fqe7 , spiol-a0a0k9r9x3 , spiol-a0a0k9rit5 , spiol-a0a0k9riw3 , spiol-a0a0k9rub8 , spiol-a0a0k9rxl0 , spiol-a0a0k9qau1 , spiol-a0a0k9rrd8 , spiol-a0a0k9qe58 , spiol-a0a0k9r322 , betvv-a0a0j8b442 , spiol-a0a0k9qnw2 , spiol-a0a0k9ri52 , spiol-a0a0k9r6e9

Title : Chinese hamster genome sequenced from sorted chromosomes -
Author(s) : Brinkrolf K , Rupp O , Laux H , Kollin F , Ernst W , Linke B , Kofler R , Romand S , Hesse F , Budach WE , Galosy S , Muller D , Noll T , Wienberg J , Jostock T , Leonard M , Grillari J , Tauch A , Goesmann A , Helk B , Mott JE , Puhler A , Borth N
Ref : Nat Biotechnol , 31 :694 , 2013
PubMedID: 23929341
Gene_locus related to this paper: crigr-a0a061igd5 , crigr-g3grm1 , crigr-g3i1j5 , crigr-a0a061ika1 , crigr-a0a061ijg4 , crigr-a0a061ihy9

Title : Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production - Bogen_2013_BMC.Genomics_14_926
Author(s) : Bogen C , Al-Dilaimi A , Albersmeier A , Wichmann J , Grundmann M , Rupp O , Lauersen KJ , Blifernez-Klassen O , Kalinowski J , Goesmann A , Mussgnug JH , Kruse O
Ref : BMC Genomics , 14 :926 , 2013
Abstract : BACKGROUND: Microalgae are gaining importance as sustainable production hosts in the fields of biotechnology and bioenergy. A robust biomass accumulating strain of the genus Monoraphidium (SAG 48.87) was investigated in this work as a potential feedstock for biofuel production. The genome was sequenced, annotated, and key enzymes for triacylglycerol formation were elucidated.
RESULTS: Monoraphidium neglectum was identified as an oleaginous species with favourable growth characteristics as well as a high potential for crude oil production, based on neutral lipid contents of approximately 21% (dry weight) under nitrogen starvation, composed of predominantly C18:1 and C16:0 fatty acids. Further characterization revealed growth in a relatively wide pH range and salt concentrations of up to 1.0% NaCl, in which the cells exhibited larger structures. This first full genome sequencing of a member of the Selenastraceae revealed a diploid, approximately 68 Mbp genome with a G + C content of 64.7%. The circular chloroplast genome was assembled to a 135,362 bp single contig, containing 67 protein-coding genes. The assembly of the mitochondrial genome resulted in two contigs with an approximate total size of 94 kb, the largest known mitochondrial genome within algae. 16,761 protein-coding genes were assigned to the nuclear genome. Comparison of gene sets with respect to functional categories revealed a higher gene number assigned to the category "carbohydrate metabolic process" and in "fatty acid biosynthetic process" in M. neglectum when compared to Chlamydomonas reinhardtii and Nannochloropsis gaditana, indicating a higher metabolic diversity for applications in carbohydrate conversions of biotechnological relevance.
CONCLUSIONS: The genome of M. neglectum, as well as the metabolic reconstruction of crucial lipid pathways, provides new insights into the diversity of the lipid metabolism in microalgae. The results of this work provide a platform to encourage the development of this strain for biotechnological applications and production concepts.
ESTHER : Bogen_2013_BMC.Genomics_14_926
PubMedSearch : Bogen_2013_BMC.Genomics_14_926
PubMedID: 24373495
Gene_locus related to this paper: 9chlo-a0a0d2mui5

Title : Establishment and interpretation of the genome sequence of the phytopathogenic fungus Rhizoctonia solani AG1-IB isolate 7\/3\/14 - Wibberg_2013_J.Biotechnol_167_142
Author(s) : Wibberg D , Jelonek L , Rupp O , Hennig M , Eikmeyer F , Goesmann A , Hartmann A , Borriss R , Grosch R , Puhler A , Schluter A
Ref : J Biotechnol , 167 :142 , 2013
Abstract : Anastomosis group AG1-IB isolates of the anamorphic basidiomycetous fungus Rhizoctonia solani Kuhn affect various agricultural and horticultural important crops including bean, rice, soybean, figs, hortensia, cabbage and lettuce. To gain insights into the genome structure and content, the first draft genome sequence of R. solani AG1-IB isolate 7/3/14 was established. Four complete runs on the Genome Sequencer (GS) FLX platform (Roche Applied Science) yielding approx. a 25-fold coverage of the R. solani genome were accomplished. Assembly of the sequence reads by means of the gsAssembler software version 2.6 applying the heterozygotic mode resulted in numerous contigs and scaffolds and a predicted size of 87.1 Mb for the diploid status of the genome. 'Contig-length vs. read-count' analysis revealed that the assembled contigs can be classified into five different groups. Detailed BLAST-analysis revealed that most contigs of group II feature high-scoring matches to other contigs of the same group suggesting that distinguishable allelic variants exist for many genes. Due to the supposed diploid and heterokaryotic nature of R. solani AG1-IB 7/3/14, this result has been anticipated. However, the heterokaryotic character of the isolate is not really supported by sequencing data obtained for the isolate R. solani AG1-IB 7/3/14. Coverage of group III contigs is twice as high as for group II contigs which can also be explained by the diploid status of the genome and indistinguishable alleles on homologous chromosomes. Assembly of sequence data led to the identification of the rRNA unit (group V contigs) and the mitochondrial (mt) genome (group IV contigs) which is a circular molecule of 162,751 bp in size featuring a GC-content of 36.4%. The R. solani 7/3/14 mt-genome is one of the largest fungal mitochondrial genomes known to date. Its large size essentially is due to the presence of numerous non-conserved hypothetical ORFs and introns. Gene prediction for the R. solani AG1-IB 7/3/14 genome was conducted by the Augustus Gene Prediction Software for Eukaryotes (version 2.6.) applying the parameter set for the fungus Coprinopsis cinerea okayama 7 130. Gene prediction and annotation provided first insights into the R. solani AG1-IB 7/3/14 gene structure and content. In total, 12,422 genes were predicted. The average number of exons per gene is five. Exons have a mean length of 214 bp, whereas introns on average are 66 bp in length. Annotation of the genome revealed that 4169 of 12,422 genes could be assigned to KOG functional categories.
ESTHER : Wibberg_2013_J.Biotechnol_167_142
PubMedSearch : Wibberg_2013_J.Biotechnol_167_142
PubMedID: 23280342
Gene_locus related to this paper: thacb-m5c801 , thacb-m5blb1 , thacb-m5bzl7 , thacb-m5c2d3

Title : Genome sequence of Wickerhamomyces anomalus DSM 6766 reveals genetic basis of biotechnologically important antimicrobial activities - Schneider_2012_FEMS.Yeast.Res_12_382
Author(s) : Schneider J , Rupp O , Trost E , Jaenicke S , Passoth V , Goesmann A , Tauch A , Brinkrolf K
Ref : FEMS Yeast Res , 12 :382 , 2012
Abstract : The ascomycetous yeast Wickerhamomyces anomalus (formerly Pichia anomala and Hansenula anomala) exhibits antimicrobial activities and flavoring features that are responsible for its frequent association with food, beverage and feed products. However, limited information on the genetic background of this yeast and its multiple capabilities are currently available. Here, we present the draft genome sequence of the neotype strain W. anomalus DSM 6766. On the basis of pyrosequencing, a de novo assembly of this strain resulted in a draft genome sequence with a total size of 25.47 Mbp. An automatic annotation using RAPYD generated 11 512 protein-coding sequences. This annotation provided the basis to analyse metabolic capabilities, phylogenetic relationships, as well as biotechnologically important features and yielded novel candidate genes of W. anomalus DSM 6766 coding for proteins participating in antimicrobial activities.
ESTHER : Schneider_2012_FEMS.Yeast.Res_12_382
PubMedSearch : Schneider_2012_FEMS.Yeast.Res_12_382
PubMedID: 22292503
Gene_locus related to this paper: hanan-h6uwn2 , hanan-h6uwn4 , hanan-h6uwn6 , hanan-h6uwn8

Title : Complete genome sequencing of Agrobacterium sp. H13-3, the former Rhizobium lupini H13-3, reveals a tripartite genome consisting of a circular and a linear chromosome and an accessory plasmid but lacking a tumor-inducing Ti-plasmid - Wibberg_2011_J.Biotechnol_155_50
Author(s) : Wibberg D , Blom J , Jaenicke S , Kollin F , Rupp O , Scharf B , Schneiker-Bekel S , Sczcepanowski R , Goesmann A , Setubal JC , Schmitt R , Puhler A , Schluter A
Ref : J Biotechnol , 155 :50 , 2011
Abstract : Agrobacterium sp. H13-3, formerly known as Rhizobium lupini H13-3, is a soil bacterium that was isolated from the rhizosphere of Lupinus luteus. The isolate has been established as a model system for studying novel features of flagellum structure, motility and chemotaxis within the family Rhizobiaceae. The complete genome sequence of Agrobacterium sp. H13-3 has been established and the genome structure and phylogenetic assignment of the organism was analysed. For de novo sequencing of the Agrobacterium sp. H13-3 genome, a combined strategy comprising 454-pyrosequencing on the Genome Sequencer FLX platform and PCR-based amplicon sequencing for gap closure was applied. The finished genome consists of three replicons and comprises 5,573,770 bases. Based on phylogenetic analyses, the isolate could be assigned to the genus Agrobacterium biovar I and represents a genomic species G1 strain within this biovariety. The highly conserved circular chromosome (2.82 Mb) of Agrobacterium sp. H13-3 mainly encodes housekeeping functions characteristic for an aerobic, heterotrophic bacterium. Agrobacterium sp. H13-3 is a motile bacterium driven by the rotation of several complex flagella. Its behaviour towards external stimuli is regulated by a large chemotaxis regulon and a total of 17 chemoreceptors. Comparable to the genome of Agrobacterium tumefaciens C58, Agrobacterium sp. H13-3 possesses a linear chromosome (2.15 Mb) that is related to its reference replicon and features chromosomal and plasmid-like properties. The accessory plasmid pAspH13-3a (0.6 Mb) is only distantly related to the plasmid pAtC58 of A. tumefaciens C58 and shows a mosaic structure. A tumor-inducing Ti-plasmid is missing in the sequenced strain H13-3 indicating that it is a non-virulent isolate.
ESTHER : Wibberg_2011_J.Biotechnol_155_50
PubMedSearch : Wibberg_2011_J.Biotechnol_155_50
PubMedID: 21329740
Gene_locus related to this paper: agrsh-f0l326 , agrsh-f0lb16 , agrsh-f0lfg1 , agrsh-f0lgt6 , agrt5-a9cfs8 , agrt5-q7d1j0 , agrt5-q7d1j3 , agrtu-ATU2126 , agrtu-ATU2481 , agrsh-f0lfk1

Title : Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis - Schneiker_2006_Nat.Biotechnol_24_997
Author(s) : Schneiker S , Martins dos Santos VA , Bartels D , Bekel T , Brecht M , Buhrmester J , Chernikova TN , Denaro R , Ferrer M , Gertler C , Goesmann A , Golyshina OV , Kaminski F , Khachane AN , Lang S , Linke B , McHardy AC , Meyer F , Nechitaylo T , Puhler A , Regenhardt D , Rupp O , Sabirova JS , Selbitschka W , Yakimov MM , Timmis KN , Vorholter FJ , Weidner S , Kaiser O , Golyshin PN
Ref : Nat Biotechnol , 24 :997 , 2006
Abstract : Alcanivorax borkumensis is a cosmopolitan marine bacterium that uses oil hydrocarbons as its exclusive source of carbon and energy. Although barely detectable in unpolluted environments, A. borkumensis becomes the dominant microbe in oil-polluted waters. A. borkumensis SK2 has a streamlined genome with a paucity of mobile genetic elements and energy generation-related genes, but with a plethora of genes accounting for its wide hydrocarbon substrate range and efficient oil-degradation capabilities. The genome further specifies systems for scavenging of nutrients, particularly organic and inorganic nitrogen and oligo-elements, biofilm formation at the oil-water interface, biosurfactant production and niche-specific stress responses. The unique combination of these features provides A. borkumensis SK2 with a competitive edge in oil-polluted environments. This genome sequence provides the basis for the future design of strategies to mitigate the ecological damage caused by oil spills.
ESTHER : Schneiker_2006_Nat.Biotechnol_24_997
PubMedSearch : Schneiker_2006_Nat.Biotechnol_24_997
PubMedID: 16878126
Gene_locus related to this paper: alcbo-Q9F9H0 , alcbs-q0vl04 , alcbs-q0vl36 , alcbs-q0vl92 , alcbs-q0vlp5 , alcbs-q0vlq1 , alcbs-q0vlt9 , alcbs-q0vm92 , alcbs-q0vmd2 , alcbs-q0vmd6 , alcbs-q0vmn9 , alcbs-q0vnu3 , alcbs-q0vp43 , alcbs-q0vpa9 , alcbs-q0vpc7 , alcbs-q0vpg7 , alcbs-q0vpn2 , alcbs-q0vps0 , alcbs-q0vq49 , alcbs-q0vsg4 , alcbs-q0vth9 , marav-a1u5n0 , alcbs-q0vp59 , alcbs-q0vlk5

Title : Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence - Thieme_2005_J.Bacteriol_187_7254
Author(s) : Thieme F , Koebnik R , Bekel T , Berger C , Boch J , Buttner D , Caldana C , Gaigalat L , Goesmann A , Kay S , Kirchner O , Lanz C , Linke B , McHardy AC , Meyer F , Mittenhuber G , Nies DH , Niesbach-Klosgen U , Patschkowski T , Ruckert C , Rupp O , Schneiker S , Schuster SC , Vorholter FJ , Weber E , Puhler A , Bonas U , Bartels D , Kaiser O
Ref : Journal of Bacteriology , 187 :7254 , 2005
Abstract : The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically important yield losses. This pathosystem has become a well-established model for studying bacterial infection strategies. Here, we present the whole-genome sequence of the pepper-pathogenic Xanthomonas campestris pv. vesicatoria strain 85-10, which comprises a 5.17-Mb circular chromosome and four plasmids. The genome has a high G+C content (64.75%) and signatures of extensive genome plasticity. Whole-genome comparisons revealed a gene order similar to both Xanthomonas axonopodis pv. citri and Xanthomonas campestris pv. campestris and a structure completely different from Xanthomonas oryzae pv. oryzae. A total of 548 coding sequences (12.2%) are unique to X. campestris pv. vesicatoria. In addition to a type III secretion system, which is essential for pathogenicity, the genome of strain 85-10 encodes all other types of protein secretion systems described so far in gram-negative bacteria. Remarkably, one of the putative type IV secretion systems encoded on the largest plasmid is similar to the Icm/Dot systems of the human pathogens Legionella pneumophila and Coxiella burnetii. Comparisons with other completely sequenced plant pathogens predicted six novel type III effector proteins and several other virulence factors, including adhesins, cell wall-degrading enzymes, and extracellular polysaccharides.
ESTHER : Thieme_2005_J.Bacteriol_187_7254
PubMedSearch : Thieme_2005_J.Bacteriol_187_7254
PubMedID: 16237009
Gene_locus related to this paper: xanac-q8pmm6 , xanax-ACVB , xanax-BIOH , xanax-CATD , xanax-CPO , xanax-DHAA , xanax-ENTF2 , xanax-GAA , xanax-META , xanax-METX , xanax-PCAD , xanax-PHBC , xanax-PTRB , xanax-Q8PMQ8 , xanax-XAC0198 , xanax-XAC0262 , xanax-XAC0319 , xanax-XAC0372 , xanax-XAC0375 , xanax-XAC0515 , xanax-XAC0574 , xanax-XAC0591 , xanax-XAC0619 , xanax-XAC0628 , xanax-XAC0736 , xanax-XAC0753 , xanax-XAC0805 , xanax-XAC0874 , xanax-XAC1200 , xanax-XAC1213 , xanax-XAC1713 , xanax-XAC2532 , xanax-XAC2541 , xanax-XAC2907 , xanax-XAC2981 , xanax-XAC2990 , xanax-XAC3037 , xanax-XAC3053 , xanax-XAC3152 , xanax-XAC3770 , xanax-XAC3999 , xanax-XAC4046 , xanax-XAC4055 , xanax-XAC4106 , xanax-XAC4221 , xanax-XAC4316 , xanax-XYNB , xanc5-q3bqi2 , xanc5-q3bst4 , xanc5-q3btn4 , xanc5-q3bup9 , xanc5-q3byx0 , xanca-estA1 , xanca-impep , xanca-XCC1105 , xanca-XCC2285 , xanca-XCC2566 , xanca-XCC2722 , xanca-XCC3164 , xanca-XCC3555 , xanor-q5h3e8

Title : Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora - Tauch_2005_J.Bacteriol_187_4671
Author(s) : Tauch A , Kaiser O , Hain T , Goesmann A , Weisshaar B , Albersmeier A , Bekel T , Bischoff N , Brune I , Chakraborty T , Kalinowski J , Meyer F , Rupp O , Schneiker S , Viehoever P , Puhler A
Ref : Journal of Bacteriology , 187 :4671 , 2005
Abstract : Corynebacterium jeikeium is a "lipophilic" and multidrug-resistant bacterial species of the human skin flora that has been recognized with increasing frequency as a serious nosocomial pathogen. Here we report the genome sequence of the clinical isolate C. jeikeium K411, which was initially recovered from the axilla of a bone marrow transplant patient. The genome of C. jeikeium K411 consists of a circular chromosome of 2,462,499 bp and the 14,323-bp bacteriocin-producing plasmid pKW4. The chromosome of C. jeikeium K411 contains 2,104 predicted coding sequences, 52% of which were considered to be orthologous with genes in the Corynebacterium glutamicum, Corynebacterium efficiens, and Corynebacterium diphtheriae genomes. These genes apparently represent the chromosomal backbone that is conserved between the four corynebacteria. Among the genes that lack an ortholog in the known corynebacterial genomes, many are located close to transposable elements or revealed an atypical G+C content, indicating that horizontal gene transfer played an important role in the acquisition of genes involved in iron and manganese homeostasis, in multidrug resistance, in bacterium-host interaction, and in virulence. Metabolic analyses of the genome sequence indicated that the "lipophilic" phenotype of C. jeikeium most likely originates from the absence of fatty acid synthase and thus represents a fatty acid auxotrophy. Accordingly, both the complete gene repertoire and the deduced lifestyle of C. jeikeium K411 largely reflect the strict dependence of growth on the presence of exogenous fatty acids. The predicted virulence factors of C. jeikeium K411 are apparently involved in ensuring the availability of exogenous fatty acids by damaging the host tissue.
ESTHER : Tauch_2005_J.Bacteriol_187_4671
PubMedSearch : Tauch_2005_J.Bacteriol_187_4671
PubMedID: 15968079
Gene_locus related to this paper: corjk-q4jsq8 , corjk-q4jst9 , corjk-q4jt28 , corjk-q4jt69 , corjk-q4jta2 , corjk-q4jth4 , corjk-q4jti5 , corjk-q4ju26 , corjk-q4jug4 , corjk-q4juz4 , corjk-q4jv41 , corjk-q4jvr2 , corjk-q4jwi5 , corjk-q4jwq5 , corjk-q4jwu9 , corjk-q4jx57 , corjk-q4jx58 , corjk-q4jxf4 , corjk-q4jxg1 , corjk-q4jxi0 , corjk-q4jxk5 , corjk-q4jxw6 , corjk-q4jxx7 , corjk-q4jy17 , corjk-q4jy19 , corjk-q4jy21 , corjk-q4jy22 , corjk-q4jyd0 , corjk-q4jyf2

Title : The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins - Kalinowski_2003_J.Biotechnol_104_5
Author(s) : Kalinowski J , Bathe B , Bartels D , Bischoff N , Bott M , Burkovski A , Dusch N , Eggeling L , Eikmanns BJ , Gaigalat L , Goesmann A , Hartmann M , Huthmacher K , Kramer R , Linke B , McHardy AC , Meyer F , Mockel B , Pfefferle W , Puhler A , Rey DA , Ruckert C , Rupp O , Sahm H , Wendisch VF , Wiegrabe I , Tauch A
Ref : J Biotechnol , 104 :5 , 2003
Abstract : The complete genomic sequence of Corynebacterium glutamicum ATCC 13032, well-known in industry for the production of amino acids, e.g. of L-glutamate and L-lysine was determined. The C. glutamicum genome was found to consist of a single circular chromosome comprising 3282708 base pairs. Several DNA regions of unusual composition were identified that were potentially acquired by horizontal gene transfer, e.g. a segment of DNA from C. diphtheriae and a prophage-containing region. After automated and manual annotation, 3002 protein-coding genes have been identified, and to 2489 of these, functions were assigned by homologies to known proteins. These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil. As an example for biotechnological application the complete genome sequence was used to reconstruct the metabolic flow of carbon into a number of industrially important products derived from the amino acid L-aspartate.
ESTHER : Kalinowski_2003_J.Biotechnol_104_5
PubMedSearch : Kalinowski_2003_J.Biotechnol_104_5
PubMedID: 12948626
Gene_locus related to this paper: corgl-CGL0007 , corgl-CGL0081 , corgl-CGL0091 , corgl-CGL0118 , corgl-CGL0151 , corgl-CGL0340 , corgl-CGL0343 , corgl-CGL0587 , corgl-CGL0591 , corgl-CGL0839 , corgl-CGL0922 , corgl-CGL1031 , corgl-Cgl1133 , corgl-CGL1144 , corgl-CGL1178 , corgl-CGL1439 , corgl-CGL1481 , corgl-CGL2002 , corgl-CGL2067 , corgl-CGL2181 , corgl-Cgl2249 , corgl-CGL2254 , corgl-CGL2393 , corgl-CGL2474 , corgl-CGL2568 , corgl-CGL2596 , corgl-CGL2618 , corgl-CGL2871 , corgl-CGL2878 , corgl-CMT3 , corgl-csp1 , corgl-q8nlm9 , corgl-q8nlr5 , corgl-q8nlz1 , corgl-y967 , corgt-g6wsn6