Richardson PM

References (17)

Title : The genome of the Western clawed frog Xenopus tropicalis - Hellsten_2010_Science_328_633
Author(s) : Hellsten U , Harland RM , Gilchrist MJ , Hendrix D , Jurka J , Kapitonov V , Ovcharenko I , Putnam NH , Shu S , Taher L , Blitz IL , Blumberg B , Dichmann DS , Dubchak I , Amaya E , Detter JC , Fletcher R , Gerhard DS , Goodstein D , Graves T , Grigoriev IV , Grimwood J , Kawashima T , Lindquist E , Lucas SM , Mead PE , Mitros T , Ogino H , Ohta Y , Poliakov AV , Pollet N , Robert J , Salamov A , Sater AK , Schmutz J , Terry A , Vize PD , Warren WC , Wells D , Wills A , Wilson RK , Zimmerman LB , Zorn AM , Grainger R , Grammer T , Khokha MK , Richardson PM , Rokhsar DS
Ref : Science , 328 :633 , 2010
Abstract : The western clawed frog Xenopus tropicalis is an important model for vertebrate development that combines experimental advantages of the African clawed frog Xenopus laevis with more tractable genetics. Here we present a draft genome sequence assembly of X. tropicalis. This genome encodes more than 20,000 protein-coding genes, including orthologs of at least 1700 human disease genes. Over 1 million expressed sequence tags validated the annotation. More than one-third of the genome consists of transposable elements, with unusually prevalent DNA transposons. Like that of other tetrapods, the genome of X. tropicalis contains gene deserts enriched for conserved noncoding elements. The genome exhibits substantial shared synteny with human and chicken over major parts of large chromosomes, broken by lineage-specific chromosome fusions and fissions, mainly in the mammalian lineage.
ESTHER : Hellsten_2010_Science_328_633
PubMedSearch : Hellsten_2010_Science_328_633
PubMedID: 20431018
Gene_locus related to this paper: xenla-q6pcj9 , xentr-a9umk0 , xentr-abhdb , xentr-ACHE , xentr-b0bm77 , xentr-b1h0y7 , xentr-b2guc4 , xentr-b7zt03 , xentr-b7ztj4 , xentr-BCHE1 , xentr-BCHE2 , xentr-cxest2 , xentr-d2x2k4 , xentr-d2x2k6 , xentr-f6rff6 , xentr-f6v0g3 , xentr-f6v2j6 , xentr-f6v3z1 , xentr-f6y4c8 , xentr-f6yve5 , xentr-f7a4y9 , xentr-f7acc5 , xentr-f7e2e2 , xentr-LOC394897 , xentr-ndrg1 , xentr-q0vfb6 , xentr-f7cpl7 , xentr-f6yj44 , xentr-f7ejk4 , xentr-f6q8j8 , xentr-f6z8f0 , xentr-f7d709 , xentr-b0bmb8 , xentr-f7af63 , xentr-a0a1b8y2w9 , xentr-f7d4k9 , xentr-f6r032 , xentr-f6yvq3 , xentr-a0a1b8y2z3 , xentr-f7afg4 , xentr-f6xb15 , xentr-f7e1r2 , xentr-a4ihf1 , xentr-f7eue5 , xentr-f6u7u3 , xentr-f172a , xentr-f7equ8 , xentr-f7dd89 , xentr-a9jtx5

Title : The genome of Polaromonas sp. strain JS666: insights into the evolution of a hydrocarbon- and xenobiotic-degrading bacterium, and features of relevance to biotechnology - Mattes_2008_Appl.Environ.Microbiol_74_6405
Author(s) : Mattes TE , Alexander AK , Richardson PM , Munk AC , Han CS , Stothard P , Coleman NV
Ref : Applied Environmental Microbiology , 74 :6405 , 2008
Abstract : Polaromonas sp. strain JS666 can grow on cis-1,2-dichloroethene (cDCE) as a sole carbon and energy source and may be useful for bioremediation of chlorinated solvent-contaminated sites. Analysis of the genome sequence of JS666 (5.9 Mb) shows a bacterium well adapted to pollution that carries many genes likely to be involved in hydrocarbon and xenobiotic catabolism and metal resistance. Clusters of genes coding for haloalkane, haloalkanoate, n-alkane, alicyclic acid, cyclic alcohol, and aromatic catabolism were analyzed in detail, and growth on acetate, catechol, chloroacetate, cyclohexane carboxylate, cyclohexanol, ferulate, heptane, 3-hydroxybenzoate, hydroxyquinol, gentisate, octane, protocatechuate, and salicylate was confirmed experimentally. Strain JS666 also harbors diverse putative mobile genetic elements, including retrons, inteins, a miniature inverted-repeat transposable element, insertion sequence transposases from 14 families, eight genomic islands, a Mu family bacteriophage, and two large (338- and 360-kb) plasmids. Both plasmids are likely to be self-transferable and carry genes for alkane, alcohol, aromatic, and haloacid metabolism. Overall, the JS666 genome sequence provides insights into the evolution of pollutant-degrading bacteria and provides a toolbox of catabolic genes with utility for biotechnology.
ESTHER : Mattes_2008_Appl.Environ.Microbiol_74_6405
PubMedSearch : Mattes_2008_Appl.Environ.Microbiol_74_6405
PubMedID: 18723656
Gene_locus related to this paper: polsj-hboh

Title : The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis - Martin_2008_Nature_452_88
Author(s) : Martin F , Aerts A , Ahren D , Brun A , Danchin EG , Duchaussoy F , Gibon J , Kohler A , Lindquist E , Pereda V , Salamov A , Shapiro HJ , Wuyts J , Blaudez D , Buee M , Brokstein P , Canback B , Cohen D , Courty PE , Coutinho PM , Delaruelle C , Detter JC , Deveau A , Difazio S , Duplessis S , Fraissinet-Tachet L , Lucic E , Frey-Klett P , Fourrey C , Feussner I , Gay G , Grimwood J , Hoegger PJ , Jain P , Kilaru S , Labbe J , Lin YC , Legue V , Le Tacon F , Marmeisse R , Melayah D , Montanini B , Muratet M , Nehls U , Niculita-Hirzel H , Oudot-Le Secq MP , Peter M , Quesneville H , Rajashekar B , Reich M , Rouhier N , Schmutz J , Yin T , Chalot M , Henrissat B , Kues U , Lucas S , Van de Peer Y , Podila GK , Polle A , Pukkila PJ , Richardson PM , Rouze P , Sanders IR , Stajich JE , Tunlid A , Tuskan G , Grigoriev IV
Ref : Nature , 452 :88 , 2008
Abstract : Mycorrhizal symbioses--the union of roots and soil fungi--are universal in terrestrial ecosystems and may have been fundamental to land colonization by plants. Boreal, temperate and montane forests all depend on ectomycorrhizae. Identification of the primary factors that regulate symbiotic development and metabolic activity will therefore open the door to understanding the role of ectomycorrhizae in plant development and physiology, allowing the full ecological significance of this symbiosis to be explored. Here we report the genome sequence of the ectomycorrhizal basidiomycete Laccaria bicolor (Fig. 1) and highlight gene sets involved in rhizosphere colonization and symbiosis. This 65-megabase genome assembly contains approximately 20,000 predicted protein-encoding genes and a very large number of transposons and repeated sequences. We detected unexpected genomic features, most notably a battery of effector-type small secreted proteins (SSPs) with unknown function, several of which are only expressed in symbiotic tissues. The most highly expressed SSP accumulates in the proliferating hyphae colonizing the host root. The ectomycorrhizae-specific SSPs probably have a decisive role in the establishment of the symbiosis. The unexpected observation that the genome of L. bicolor lacks carbohydrate-active enzymes involved in degradation of plant cell walls, but maintains the ability to degrade non-plant cell wall polysaccharides, reveals the dual saprotrophic and biotrophic lifestyle of the mycorrhizal fungus that enables it to grow within both soil and living plant roots. The predicted gene inventory of the L. bicolor genome, therefore, points to previously unknown mechanisms of symbiosis operating in biotrophic mycorrhizal fungi. The availability of this genome provides an unparalleled opportunity to develop a deeper understanding of the processes by which symbionts interact with plants within their ecosystem to perform vital functions in the carbon and nitrogen cycles that are fundamental to sustainable plant productivity.
ESTHER : Martin_2008_Nature_452_88
PubMedSearch : Martin_2008_Nature_452_88
PubMedID: 18322534
Gene_locus related to this paper: lacbs-b0cns1 , lacbs-b0cpl4 , lacbs-b0cr62 , lacbs-b0cr66 , lacbs-b0csq9 , lacbs-b0ct56 , lacbs-b0ctt5 , lacbs-b0cuw1 , lacbs-b0cv23 , lacbs-b0cxm7 , lacbs-b0cz37 , lacbs-b0czx3 , lacbs-b0d0z5 , lacbs-b0d4i0 , lacbs-b0d4j3 , lacbs-b0d5n6 , lacbs-b0d8k0 , lacbs-b0d263 , lacbs-b0dhh1 , lacbs-b0dkp6 , lacbs-b0dmr2 , lacbs-b0dmt4 , lacbs-b0dsx5 , lacbs-b0dt05 , lacbs-b0dtw4 , lacbs-b0du88 , lacbs-b0dsl6

Title : Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth - Lee_2008_BMC.Genomics_9_247
Author(s) : Lee JH , Karamychev VN , Kozyavkin SA , Mills D , Pavlov AR , Pavlova NV , Polouchine NN , Richardson PM , Shakhova VV , Slesarev AI , Weimer B , O'Sullivan DJ
Ref : BMC Genomics , 9 :247 , 2008
Abstract : BACKGROUND: Bifidobacteria are frequently proposed to be associated with good intestinal health primarily because of their overriding dominance in the feces of breast fed infants. However, clinical feeding studies with exogenous bifidobacteria show they don't remain in the intestine, suggesting they may lose competitive fitness when grown outside the gut. RESULTS: To further the understanding of genetic attenuation that may be occurring in bifidobacteria cultures, we obtained the complete genome sequence of an intestinal isolate, Bifidobacterium longum DJO10A that was minimally cultured in the laboratory, and compared it to that of a culture collection strain, B. longum NCC2705. This comparison revealed colinear genomes that exhibited high sequence identity, except for the presence of 17 unique DNA regions in strain DJO10A and six in strain NCC2705. While the majority of these unique regions encoded proteins of diverse function, eight from the DJO10A genome and one from NCC2705, encoded gene clusters predicted to be involved in diverse traits pertinent to the human intestinal environment, specifically oligosaccharide and polyol utilization, arsenic resistance and lantibiotic production. Seven of these unique regions were suggested by a base deviation index analysis to have been precisely deleted from strain NCC2705 and this is substantiated by a DNA remnant from within one of the regions still remaining in the genome of NCC2705 at the same locus. This targeted loss of genomic regions was experimentally validated when growth of the intestinal B. longum in the laboratory for 1,000 generations resulted in two large deletions, one in a lantibiotic encoding region, analogous to a predicted deletion event for NCC2705. A simulated fecal growth study showed a significant reduced competitive ability of this deletion strain against Clostridium difficile and E. coli. The deleted region was between two IS30 elements which were experimentally demonstrated to be hyperactive within the genome. The other deleted region bordered a novel class of mobile elements, termed mobile integrase cassettes (MIC) substantiating the likely role of these elements in genome deletion events. CONCLUSION: Deletion of genomic regions, often facilitated by mobile elements, allows bifidobacteria to adapt to fermentation environments in a very rapid manner (2 genome deletions per 1,000 generations) and the concomitant loss of possible competitive abilities in the gut.
ESTHER : Lee_2008_BMC.Genomics_9_247
PubMedSearch : Lee_2008_BMC.Genomics_9_247
PubMedID: 18505588
Gene_locus related to this paper: bifli-c2gxu7 , biflo-BL0073 , biflo-BL0336 , biflo-BL0581 , biflo-BL0582 , biflo-BL0682 , biflo-BL0787 , biflo-BL0807 , biflo-BL1109 , biflo-BL1514 , biflo-PAP , biflo-PTRB

Title : The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome - Sela_2008_Proc.Natl.Acad.Sci.U.S.A_105_18964
Author(s) : Sela DA , Chapman J , Adeuya A , Kim JH , Chen F , Whitehead TR , Lapidus A , Rokhsar DS , Lebrilla CB , German JB , Price NP , Richardson PM , Mills DA
Ref : Proc Natl Acad Sci U S A , 105 :18964 , 2008
Abstract : Following birth, the breast-fed infant gastrointestinal tract is rapidly colonized by a microbial consortium often dominated by bifidobacteria. Accordingly, the complete genome sequence of Bifidobacterium longum subsp. infantis ATCC15697 reflects a competitive nutrient-utilization strategy targeting milk-borne molecules which lack a nutritive value to the neonate. Several chromosomal loci reflect potential adaptation to the infant host including a 43 kbp cluster encoding catabolic genes, extracellular solute binding proteins and permeases predicted to be active on milk oligosaccharides. An examination of in vivo metabolism has detected the hallmarks of milk oligosaccharide utilization via the central fermentative pathway using metabolomic and proteomic approaches. Finally, conservation of gene clusters in multiple isolates corroborates the genomic mechanism underlying milk utilization for this infant-associated phylotype.
ESTHER : Sela_2008_Proc.Natl.Acad.Sci.U.S.A_105_18964
PubMedSearch : Sela_2008_Proc.Natl.Acad.Sci.U.S.A_105_18964
PubMedID: 19033196
Gene_locus related to this paper: bifli-c2gxu7 , biflo-BL0073 , biflo-BL0336 , biflo-BL0581 , biflo-BL0582 , biflo-BL0787 , biflo-BL0807 , biflo-BL1514 , biflo-PTRB , bifln-c2gtr2

Title : Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40 T - Weiner_2008_PLoS.Genet_4_e1000087
Author(s) : Weiner RM , Taylor LE, 2nd , Henrissat B , Hauser L , Land M , Coutinho PM , Rancurel C , Saunders EH , Longmire AG , Zhang H , Bayer EA , Gilbert HJ , Larimer F , Zhulin IB , Ekborg NA , Lamed R , Richardson PM , Borovok I , Hutcheson S
Ref : PLoS Genet , 4 :e1000087 , 2008
Abstract : The marine bacterium Saccharophagus degradans strain 2-40 (Sde 2-40) is emerging as a vanguard of a recently discovered group of marine and estuarine bacteria that recycles complex polysaccharides. We report its complete genome sequence, analysis of which identifies an unusually large number of enzymes that degrade >10 complex polysaccharides. Not only is this an extraordinary range of catabolic capability, many of the enzymes exhibit unusual architecture including novel combinations of catalytic and substrate-binding modules. We hypothesize that many of these features are adaptations that facilitate depolymerization of complex polysaccharides in the marine environment. This is the first sequenced genome of a marine bacterium that can degrade plant cell walls, an important component of the carbon cycle that is not well-characterized in the marine environment.
ESTHER : Weiner_2008_PLoS.Genet_4_e1000087
PubMedSearch : Weiner_2008_PLoS.Genet_4_e1000087
PubMedID: 18516288
Gene_locus related to this paper: sacd2-q21f03 , sacd2-q21l72 , sacd2-q21ms2 , sacd2-q21ll5

Title : Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1 - Kane_2007_J.Bacteriol_189_1931
Author(s) : Kane SR , Chakicherla AY , Chain PS , Schmidt R , Shin MW , Legler TC , Scow KM , Larimer FW , Lucas SM , Richardson PM , Hristova KR
Ref : Journal of Bacteriology , 189 :1931 , 2007
Abstract : Methylibium petroleiphilum PM1 is a methylotroph distinguished by its ability to completely metabolize the fuel oxygenate methyl tert-butyl ether (MTBE). Strain PM1 also degrades aromatic (benzene, toluene, and xylene) and straight-chain (C(5) to C(12)) hydrocarbons present in petroleum products. Whole-genome analysis of PM1 revealed an approximately 4-Mb circular chromosome and an approximately 600-kb megaplasmid, containing 3,831 and 646 genes, respectively. Aromatic hydrocarbon and alkane degradation, metal resistance, and methylotrophy are encoded on the chromosome. The megaplasmid contains an unusual t-RNA island, numerous insertion sequences, and large repeated elements, including a 40-kb region also present on the chromosome and a 29-kb tandem repeat encoding phosphonate transport and cobalamin biosynthesis. The megaplasmid also codes for alkane degradation and was shown to play an essential role in MTBE degradation through plasmid-curing experiments. Discrepancies between the insertion sequence element distribution patterns, the distributions of best BLASTP hits among major phylogenetic groups, and the G+C contents of the chromosome (69.2%) and plasmid (66%), together with comparative genome hybridization experiments, suggest that the plasmid was recently acquired and apparently carries the genetic information responsible for PM1's ability to degrade MTBE. Comparative genomic hybridization analysis with two PM1-like MTBE-degrading environmental isolates (approximately 99% identical 16S rRNA gene sequences) showed that the plasmid was highly conserved (ca. 99% identical), whereas the chromosomes were too diverse to conduct resequencing analysis. PM1's genome sequence provides a foundation for investigating MTBE biodegradation and exploring the genetic regulation of multiple biodegradation pathways in M. petroleiphilum and other MTBE-degrading beta-proteobacteria.
ESTHER : Kane_2007_J.Bacteriol_189_1931
PubMedSearch : Kane_2007_J.Bacteriol_189_1931
PubMedID: 17158667
Gene_locus related to this paper: metpp-a2scm2 , metpp-a2set2 , metpp-a2sg50 , metpp-a2sg73 , metpp-a2si41 , metpp-a2siz8 , metpp-a2skw2 , metpp-a2slp5 , metpp-a2slq1 , metpp-a2slq8 , metpp-a2sly3 , metpp-metx , metpp-a2smg5 , metpp-a2skr2 , metpp-a2sg36 , metpp-a2sjv0

Title : The Calyptogena magnifica chemoautotrophic symbiont genome - Newton_2007_Science_315_998
Author(s) : Newton IL , Woyke T , Auchtung TA , Dilly GF , Dutton RJ , Fisher MC , Fontanez KM , Lau E , Stewart FJ , Richardson PM , Barry KW , Saunders E , Detter JC , Wu D , Eisen JA , Cavanaugh CM
Ref : Science , 315 :998 , 2007
Abstract : Chemoautotrophic endosymbionts are the metabolic cornerstone of hydrothermal vent communities, providing invertebrate hosts with nearly all of their nutrition. The Calyptogena magnifica (Bivalvia: Vesicomyidae) symbiont, Candidatus Ruthia magnifica, is the first intracellular sulfur-oxidizing endosymbiont to have its genome sequenced, revealing a suite of metabolic capabilities. The genome encodes major chemoautotrophic pathways as well as pathways for biosynthesis of vitamins, cofactors, and all 20 amino acids required by the clam.
ESTHER : Newton_2007_Science_315_998
PubMedSearch : Newton_2007_Science_315_998
PubMedID: 17303757
Gene_locus related to this paper: rutmc-a1aw39 , rutmc-a1ax96

Title : Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis - Jeffries_2007_Nat.Biotechnol_25_319
Author(s) : Jeffries TW , Grigoriev IV , Grimwood J , Laplaza JM , Aerts A , Salamov A , Schmutz J , Lindquist E , Dehal P , Shapiro H , Jin YS , Passoth V , Richardson PM
Ref : Nat Biotechnol , 25 :319 , 2007
Abstract : Xylose is a major constituent of plant lignocellulose, and its fermentation is important for the bioconversion of plant biomass to fuels and chemicals. Pichia stipitis is a well-studied, native xylose-fermenting yeast. The mechanism and regulation of xylose metabolism in P. stipitis have been characterized and genes from P. stipitis have been used to engineer xylose metabolism in Saccharomyces cerevisiae. We have sequenced and assembled the complete genome of P. stipitis. The sequence data have revealed unusual aspects of genome organization, numerous genes for bioconversion, a preliminary insight into regulation of central metabolic pathways and several examples of colocalized genes with related functions. The genome sequence provides insight into how P. stipitis regulates its redox balance while very efficiently fermenting xylose under microaerobic conditions.
ESTHER : Jeffries_2007_Nat.Biotechnol_25_319
PubMedSearch : Jeffries_2007_Nat.Biotechnol_25_319
PubMedID: 17334359
Gene_locus related to this paper: picst-a3geu9 , picst-a3gfu2 , picst-a3ggh9 , picst-a3gha8 , picst-a3ghe3 , picst-a3gi73 , picst-a3lmu3 , picst-a3ln06 , picst-a3ln59 , picst-a3lnv8 , picst-a3lp77 , picst-a3lqt4 , picst-a3lrt0 , picst-a3ls15 , picst-a3lsj8 , picst-a3lu11 , picst-a3luu0 , picst-a3lv87 , picst-a3lvi5 , picst-a3lvu9 , picst-a3lvv2 , picst-a3lwa4 , picst-a3lxl2 , picst-a3lxs8 , picst-a3lyi3 , picst-atg15 , picst-bna7 , picst-a3lyh1 , picst-a3lnc5 , picst-a3lr32

Title : Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum - Hallam_2006_Proc.Natl.Acad.Sci.U.S.A_103_18296
Author(s) : Hallam SJ , Konstantinidis KT , Putnam N , Schleper C , Watanabe Y , Sugahara J , Preston C , de la Torre J , Richardson PM , DeLong EF
Ref : Proc Natl Acad Sci U S A , 103 :18296 , 2006
Abstract : Crenarchaeota are ubiquitous and abundant microbial constituents of soils, sediments, lakes, and ocean waters. To further describe the cosmopolitan nonthermophilic Crenarchaeota, we analyzed the genome sequence of one representative, the uncultivated sponge symbiont Cenarchaeum symbiosum. C. symbiosum genotypes coinhabiting the same host partitioned into two dominant populations, corresponding to previously described a- and b-type ribosomal RNA variants. Although they were syntenic, overlapping a- and b-type ribotype genomes harbored significant variability. A single tiling path comprising the dominant a-type genotype was assembled and used to explore the genomic properties of C. symbiosum and its planktonic relatives. Of 2,066 ORFs, 55.6% matched genes with predicted function from previously sequenced genomes. The remaining genes partitioned between functional RNAs (2.4%) and hypotheticals (42%) with limited homology to known functional genes. The latter category included some genes likely involved in the archaeal-sponge symbiotic association. Conversely, 525 C. symbiosum ORFs were most highly similar to sequences from marine environmental genomic surveys, and they apparently represent orthologous genes from free-living planktonic Crenarchaeota. In total, the C. symbiosum genome was remarkably distinct from those of other known Archaea and shared many core metabolic features in common with its free-living planktonic relatives.
ESTHER : Hallam_2006_Proc.Natl.Acad.Sci.U.S.A_103_18296
PubMedSearch : Hallam_2006_Proc.Natl.Acad.Sci.U.S.A_103_18296
PubMedID: 17114289
Gene_locus related to this paper: censy-a0rvz5 , censy-a0rx82 , censy-a0rx85 , censy-a0rxe4

Title : The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies - Mavromatis_2006_J.Bacteriol_188_4015
Author(s) : Mavromatis K , Doyle CK , Lykidis A , Ivanova N , Francino MP , Chain P , Shin M , Malfatti S , Larimer F , Copeland A , Detter JC , Land M , Richardson PM , Yu XJ , Walker DH , McBride JW , Kyrpides NC
Ref : Journal of Bacteriology , 188 :4015 , 2006
Abstract : Ehrlichia canis, a small obligately intracellular, tick-transmitted, gram-negative, alpha-proteobacterium, is the primary etiologic agent of globally distributed canine monocytic ehrlichiosis. Complete genome sequencing revealed that the E. canis genome consists of a single circular chromosome of 1,315,030 bp predicted to encode 925 proteins, 40 stable RNA species, 17 putative pseudogenes, and a substantial proportion of noncoding sequence (27%). Interesting genome features include a large set of proteins with transmembrane helices and/or signal sequences and a unique serine-threonine bias associated with the potential for O glycosylation that was prominent in proteins associated with pathogen-host interactions. Furthermore, two paralogous protein families associated with immune evasion were identified, one of which contains poly(G-C) tracts, suggesting that they may play a role in phase variation and facilitation of persistent infections. Genes associated with pathogen-host interactions were identified, including a small group encoding proteins (n = 12) with tandem repeats and another group encoding proteins with eukaryote-like ankyrin domains (n = 7).
ESTHER : Mavromatis_2006_J.Bacteriol_188_4015
PubMedSearch : Mavromatis_2006_J.Bacteriol_188_4015
PubMedID: 16707693
Gene_locus related to this paper: ehrcj-q3ys50

Title : The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans - Beller_2006_J.Bacteriol_188_1473
Author(s) : Beller HR , Chain PS , Letain TE , Chakicherla A , Larimer FW , Richardson PM , Coleman MA , Wood AP , Kelly DP
Ref : Journal of Bacteriology , 188 :1473 , 2006
Abstract : The complete genome sequence of Thiobacillus denitrificans ATCC 25259 is the first to become available for an obligately chemolithoautotrophic, sulfur-compound-oxidizing, beta-proteobacterium. Analysis of the 2,909,809-bp genome will facilitate our molecular and biochemical understanding of the unusual metabolic repertoire of this bacterium, including its ability to couple denitrification to sulfur-compound oxidation, to catalyze anaerobic, nitrate-dependent oxidation of Fe(II) and U(IV), and to oxidize mineral electron donors. Notable genomic features include (i) genes encoding c-type cytochromes totaling 1 to 2 percent of the genome, which is a proportion greater than for almost all bacterial and archaeal species sequenced to date, (ii) genes encoding two [NiFe]hydrogenases, which is particularly significant because no information on hydrogenases has previously been reported for T. denitrificans and hydrogen oxidation appears to be critical for anaerobic U(IV) oxidation by this species, (iii) a diverse complement of more than 50 genes associated with sulfur-compound oxidation (including sox genes, dsr genes, and genes associated with the AMP-dependent oxidation of sulfite to sulfate), some of which occur in multiple (up to eight) copies, (iv) a relatively large number of genes associated with inorganic ion transport and heavy metal resistance, and (v) a paucity of genes encoding organic-compound transporters, commensurate with obligate chemolithoautotrophy. Ultimately, the genome sequence of T. denitrificans will enable elucidation of the mechanisms of aerobic and anaerobic sulfur-compound oxidation by beta-proteobacteria and will help reveal the molecular basis of this organism's role in major biogeochemical cycles (i.e., those involving sulfur, nitrogen, and carbon) and groundwater restoration.
ESTHER : Beller_2006_J.Bacteriol_188_1473
PubMedSearch : Beller_2006_J.Bacteriol_188_1473
PubMedID: 16452431
Gene_locus related to this paper: thida-q3sez3 , thida-q3sfi8 , thida-q3sfs9 , thida-q3sgm5 , thida-q3sh03 , thida-q3sij1 , thida-q3sja9 , thida-q3sk45 , thida-q3slh7 , thida-q3slx8

Title : Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000 - Feil_2005_Proc.Natl.Acad.Sci.U.S.A_102_11064
Author(s) : Feil H , Feil WS , Chain P , Larimer F , DiBartolo G , Copeland A , Lykidis A , Trong S , Nolan M , Goltsman E , Thiel J , Malfatti S , Loper JE , Lapidus A , Detter JC , Land M , Richardson PM , Kyrpides NC , Ivanova N , Lindow SE
Ref : Proc Natl Acad Sci U S A , 102 :11064 , 2005
Abstract : The complete genomic sequence of Pseudomonas syringae pv. syringae B728a (Pss B728a) has been determined and is compared with that of P. syringae pv. tomato DC3000 (Pst DC3000). The two pathovars of this economically important species of plant pathogenic bacteria differ in host range and other interactions with plants, with Pss having a more pronounced epiphytic stage of growth and higher abiotic stress tolerance and Pst DC3000 having a more pronounced apoplastic growth habitat. The Pss B728a genome (6.1 Mb) contains a circular chromosome and no plasmid, whereas the Pst DC3000 genome is 6.5 mbp in size, composed of a circular chromosome and two plasmids. Although a high degree of similarity exists between the two sequenced Pseudomonads, 976 protein-encoding genes are unique to Pss B728a when compared with Pst DC3000, including large genomic islands likely to contribute to virulence and host specificity. Over 375 repetitive extragenic palindromic sequences unique to Pss B728a when compared with Pst DC3000 are widely distributed throughout the chromosome except in 14 genomic islands, which generally had lower GC content than the genome as a whole. Content of the genomic islands varies, with one containing a prophage and another the plasmid pKLC102 of Pseudomonas aeruginosa PAO1. Among the 976 genes of Pss B728a with no counterpart in Pst DC3000 are those encoding for syringopeptin, syringomycin, indole acetic acid biosynthesis, arginine degradation, and production of ice nuclei. The genomic comparison suggests that several unique genes for Pss B728a such as ectoine synthase, DNA repair, and antibiotic production may contribute to the epiphytic fitness and stress tolerance of this organism.
ESTHER : Feil_2005_Proc.Natl.Acad.Sci.U.S.A_102_11064
PubMedSearch : Feil_2005_Proc.Natl.Acad.Sci.U.S.A_102_11064
PubMedID: 16043691
Gene_locus related to this paper: psesm-METX , psesm-q87y20 , psesm-q889k3 , psesy-PIP , psesy-PSPTO2134 , psesy-PSPTO3135 , psesy-SYLD , psesy-SYPC , psesy-SYRE , pseu2-q4zlt8 , pseu2-q4zm40 , pseu2-q4zmb0 , pseu2-q4zmc0 , pseu2-q4zmh2 , pseu2-q4zmw4 , pseu2-q4zn59 , pseu2-q4znb2 , pseu2-q4znm5 , pseu2-q4zpb0 , pseu2-q4zph7 , pseu2-q4zpw6 , pseu2-q4zq18 , pseu2-q4zq29 , pseu2-q4zqk3 , pseu2-q4zr46 , pseu2-q4zrk3 , pseu2-q4zrq0 , pseu2-q4zrr1 , pseu2-q4zrt9 , pseu2-q4zs33 , pseu2-q4zs84 , pseu2-q4zs88 , pseu2-q4zsh3 , pseu2-q4zt45 , pseu2-q4zt74 , pseu2-q4zta6 , pseu2-q4zte9 , pseu2-q4zts5 , pseu2-q4zun8 , pseu2-q4zuv2 , pseu2-q4zuw1 , pseu2-q4zv19 , pseu2-q4zv57 , pseu2-q4zvi1 , pseu2-q4zvi2 , pseu2-q4zw50 , pseu2-q4zwq3 , pseu2-q4zwv7 , pseu2-q4zxj5 , pseu2-q4zxs0 , pseu2-q4zz02 , pseu2-q4zzm1 , pseu2-q4zzm4 , pseu2-q500r7 , pseu2-q4zqx8

Title : The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism - Armbrust_2004_Science_306_79
Author(s) : Armbrust EV , Berges JA , Bowler C , Green BR , Martinez D , Putnam NH , Zhou S , Allen AE , Apt KE , Bechner M , Brzezinski MA , Chaal BK , Chiovitti A , Davis AK , Demarest MS , Detter JC , Glavina T , Goodstein D , Hadi MZ , Hellsten U , Hildebrand M , Jenkins BD , Jurka J , Kapitonov VV , Kroger N , Lau WW , Lane TW , Larimer FW , Lippmeier JC , Lucas S , Medina M , Montsant A , Obornik M , Parker MS , Palenik B , Pazour GJ , Richardson PM , Rynearson TA , Saito MA , Schwartz DC , Thamatrakoln K , Valentin K , Vardi A , Wilkerson FP , Rokhsar DS
Ref : Science , 306 :79 , 2004
Abstract : Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. They are responsible for approximately 20% of global carbon fixation. We report the 34 million-base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand-base pair plastid and 44 thousand-base pair mitochondrial genomes. Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. We identified novel genes for silicic acid transport and formation of silica-based cell walls, high-affinity iron uptake, biosynthetic enzymes for several types of polyunsaturated fatty acids, use of a range of nitrogenous compounds, and a complete urea cycle, all attributes that allow diatoms to prosper in aquatic environments.
ESTHER : Armbrust_2004_Science_306_79
PubMedSearch : Armbrust_2004_Science_306_79
PubMedID: 15459382
Gene_locus related to this paper: thaps-b5ymy7 , thaps-b5yn04 , thaps-b5ynz7 , thaps-b8bq57 , thaps-b8bsn5 , thaps-b8bsy4 , thaps-b8bv00 , thaps-b8bxb3 , thaps-b8byx0 , thaps-b8bzg5 , thaps-b8c0a3 , thaps-b8c2d8 , thaps-b8c2k9 , thaps-b8c2s5 , thaps-b8c3p0 , thaps-b8c5l7 , thaps-b8c6y7 , thaps-b8c9k8 , thaps-b8c9t6 , thaps-b8c345 , thaps-b8c584 , thaps-b8c885 , thaps-b8c954 , thaps-b8cdd7 , thaps-b8cdt3 , thaps-b8cf07 , thaps-b8cfn8 , thaps-b8c079

Title : Reverse methanogenesis: testing the hypothesis with environmental genomics - Hallam_2004_Science_305_1457
Author(s) : Hallam SJ , Putnam N , Preston CM , Detter JC , Rokhsar D , Richardson PM , DeLong EF
Ref : Science , 305 :1457 , 2004
Abstract : Microbial methane consumption in anoxic sediments significantly impacts the global environment by reducing the flux of greenhouse gases from ocean to atmosphere. Despite its significance, the biological mechanisms controlling anaerobic methane oxidation are not well characterized. One current model suggests that relatives of methane-producing Archaea developed the capacity to reverse methanogenesis and thereby to consume methane to produce cellular carbon and energy. We report here a test of the "reverse-methanogenesis" hypothesis by genomic analyses of methane-oxidizing Archaea from deep-sea sediments. Our results show that nearly all genes typically associated with methane production are present in one specific group of archaeal methanotrophs. These genome-based observations support previous hypotheses and provide an informed foundation for metabolic modeling of anaerobic methane oxidation.
ESTHER : Hallam_2004_Science_305_1457
PubMedSearch : Hallam_2004_Science_305_1457
PubMedID: 15353801
Gene_locus related to this paper: 9arch-q64ad0 , 9arch-q64bc9 , 9arch-q648n1

Title : Community structure and metabolism through reconstruction of microbial genomes from the environment - Tyson_2004_Nature_428_37
Author(s) : Tyson GW , Chapman J , Hugenholtz P , Allen EE , Ram RJ , Richardson PM , Solovyev VV , Rubin EM , Rokhsar DS , Banfield JF
Ref : Nature , 428 :37 , 2004
Abstract : Microbial communities are vital in the functioning of all ecosystems; however, most microorganisms are uncultivated, and their roles in natural systems are unclear. Here, using random shotgun sequencing of DNA from a natural acidophilic biofilm, we report reconstruction of near-complete genomes of Leptospirillum group II and Ferroplasma type II, and partial recovery of three other genomes. This was possible because the biofilm was dominated by a small number of species populations and the frequency of genomic rearrangements and gene insertions or deletions was relatively low. Because each sequence read came from a different individual, we could determine that single-nucleotide polymorphisms are the predominant form of heterogeneity at the strain level. The Leptospirillum group II genome had remarkably few nucleotide polymorphisms, despite the existence of low-abundance variants. The Ferroplasma type II genome seems to be a composite from three ancestral strains that have undergone homologous recombination to form a large population of mosaic genomes. Analysis of the gene complement for each organism revealed the pathways for carbon and nitrogen fixation and energy generation, and provided insights into survival strategies in an extreme environment.
ESTHER : Tyson_2004_Nature_428_37
PubMedSearch : Tyson_2004_Nature_428_37
PubMedID: 14961025

Title : Differentiation effects of ciliary neurotrophic factor on human neuroblastoma cells - Lawrance_1995_J.Neurochem_64_1483
Author(s) : Lawrance G , Rylett RJ , Richardson PM , Dunn RJ , Dow KE , Riopelle RJ
Ref : Journal of Neurochemistry , 64 :1483 , 1995
Abstract : In the human neuroblastoma cell line LA-N-2, recombinant rat ciliary neurotrophic factor (CNTF) induced neurite growth and cholinergic differentiation that were both half-maximally saturated at < 100 pM of the neurokine, but was not required for cell survival in serum-free conditions over a 13-day period. CNTF markedly stimulated choline acetyltransferase activity and acetylcholine synthesis, whereas high-affinity choline transport was only slightly enhanced and acetylcholinesterase activity was unchanged. Leukemia inhibitory factor had effects identical to CNTF on neurite growth and choline acetyltransferase activity, but interleukin 6 had no effect. Radioiodinated CNTF binding and affinity cross-linking studies were consistent with tripartite receptor activation as a mediator of the observed biological effects.
ESTHER : Lawrance_1995_J.Neurochem_64_1483
PubMedSearch : Lawrance_1995_J.Neurochem_64_1483
PubMedID: 7891074