Ji C

References (15)

Title : A new plant-esterase inhibition based electrochemical sensor with signal amplification by MoS(2)@N-CDs for chlorpyrifos detection - Chen_2024_RSC.Adv_14_10703
Author(s) : Chen J , Ji C , Wang X , Tian Y , Tao H
Ref : RSC Adv , 14 :10703 , 2024
Abstract : Chlorpyrifos (CPF) is the most common pesticide entering the food chain and posing a threat to human health. This study presents a new electrochemical biosensor based on molybdenum disulfide nanosheets and nitrogen-doped carbon dot nanocomposite (MoS(2)@N-CDs) and kidney bean esterase (KdBE), and it is shown to achieve accurate detection of CPF. MoS(2)@N-CDs were prepared by a facile solvothermal method and characterized by electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Electrochemical characterization confirmed that MoS(2)@N-CDs facilitated electron transfer and increased the electroactive surface area of the electrode, thereby improved the sensing performance of the electrode. The oxidation peak current of 1-naphthol, which was produced by the hydrolysis of 1-naphthyl acetate catalyzed by KdBE, was adopted as the signal of the sensor. CPF can suppress KdBE activity and consequently cause a decrease in the sensing signal. The experimental results show that the variation of sensing signal is a reliable index to evaluate the CPF level. Under the optimized conditions, the developed enzyme sensor showed superior CPF assay performance with a linear detection range as wide as 0.01-500 microg L(-1) and LOD as low as 3.5 x 10(-3) microg L(-1) (S/N = 3). The inter- and intra-batch RSDs for electrode testing were 4.02% and 2.69%, respectively. Moreover, the developed biosensor also showed good stability and anti-interference. The spiked recoveries of CPF in oilseed rape and cabbage ranged from 98.09% to 106.01% with low relative standard deviation (RSD) (<5.23%), suggesting that the sensor is a promising tool to enable simple, low-cost but highly sensitive large-scale screening of CPF residues in food.
ESTHER : Chen_2024_RSC.Adv_14_10703
PubMedSearch : Chen_2024_RSC.Adv_14_10703
PubMedID: 38567337

Title : A Multienzyme Reaction-Mediated Electrochemical Biosensor for Sensitive Detection of Organophosphorus Pesticides - Ji_2024_Biosensors.(Basel)_14_
Author(s) : Ji C , Tang X , Wen R , Xu C , Wei J , Han B , Wu L
Ref : Biosensors (Basel) , 14 : , 2024
Abstract : Ethephon (ETH), a commonly employed growth regulator, poses potential health risks due to its residue in fruits and vegetables, leading to both acute and subchronic toxicity. However, the detection accuracy of ETH is compromised by the color effects of the samples during the detection process. In this work, a multienzyme reaction-mediated electrochemical biosensor (MRMEC) was developed for the sensitive, rapid, and color-interference-resistant determination of ETH. Nanozymes Fe(3)O(4)@Au-Pt and graphene nanocomplexes (GN-Au NPs) were prepared as catalysts and signal amplifiers for MRMEC. Acetylcholinesterase (AChE), acetylcholine (ACh), and choline oxidase (CHOx) form a cascade enzyme reaction to produce H(2)O(2) in an electrolytic cell. Fe(3)O(4)@Au-Pt has excellent peroxidase-like activity and can catalyze the oxidation of 3,3',5,5'-tetramethvlbenzidine (TMB) in the presence of H(2)O(2), resulting in a decrease in the characteristic peak current of TMB. Based on the inhibitory effect of ETH on AChE, the differential pulse voltammetry (DPV) current signal of TMB was used to detect ETH, offering the limit of detection (LOD) of 2.01 nmol L(-1). The MRMEC method effectively analyzed ETH levels in mangoes, showing satisfactory precision (coefficient of variations, 2.88-15.97%) and recovery rate (92.18-110.72%). This biosensor holds promise for detecting various organophosphorus pesticides in food samples.
ESTHER : Ji_2024_Biosensors.(Basel)_14_
PubMedSearch : Ji_2024_Biosensors.(Basel)_14_
PubMedID: 38391981

Title : Toxicological mechanism of cadmium in the clam Ruditapes philippinarum using combined ionomic, metabolomic and transcriptomic analyses - Zhang_2023_Environ.Pollut__121286
Author(s) : Zhang X , Li F , Ji C , Wu H
Ref : Environ Pollut , :121286 , 2023
Abstract : Cadmium (Cd) contamination in marine environment poses great risks to the organisms due to its potential adverse effects. In the present study, the toxicological effects and mechanisms of Cd at environmentally relevant concentrations (5 and 50 microg/L) on clam Ruditapes philippinarum after 21 days were investigated by combined ionomic, metabolomic, and transcriptomic analyses. Results showed that the uptake of Cd significantly decreased the concentrations of Cu, Zn, Sr, Se, and Mo in the whole soft tissue from 50 microg/L Cd-treated clams. Significantly negative correlations were observed between Cd and essential elements (Zn, Sr, Se, and Mo). Altered essential elements homeostasis was associated with the gene regulation of transport and detoxification, including ATP-binding cassette protein subfamily B member 1 (ABCB1) and metallothioneins (MT). The crucial contribution of Se to Cd detoxification was also found in clams. Additionally, gene set enrichment analysis showed that Cd could interfere with proteolysis by peptidases and decrease the translation efficiency at 50 microg/L. Cd inhibited lipid metabolism in clams and increased energy demand by up-regulating glycolysis and TCA cycle. Osmotic pressure was regulated by free amino acids, including alanine, glutamate, taurine, and homarine. Meanwhile, significant alterations of some differentially expressed genes, such as dopamine-beta-hydroxylase (DBH), neuroligin (NLGN), NOTCH 1, and chondroitin sulfate proteoglycan 1 (CSPG1) were observed in clams, which implied potential interference with synaptic transmission. Overall, through integrating multiple omics, this study provided new insights into the toxicological mechanisms of Cd, particularly in those mediated by dysregulation of essential element homeostasis.
ESTHER : Zhang_2023_Environ.Pollut__121286
PubMedSearch : Zhang_2023_Environ.Pollut__121286
PubMedID: 36791949

Title : Polyketide Derivatives from the Endophytic Fungus Phaeosphaeria sp. LF5 Isolated from Huperzia serrata and Their Acetylcholinesterase Inhibitory Activities - Xiao_2022_J.Fungi.(Basel)_8_
Author(s) : Xiao Y , Liang W , Zhang Z , Wang Y , Zhang S , Liu J , Chang J , Ji C , Zhu D
Ref : J Fungi (Basel) , 8 : , 2022
Abstract : The secondary metabolites of Phaeosphaeria sp. LF5, an endophytic fungus with acetylcholinesterase (AChE) inhibitory activity isolated from Huperzia serrata, were investigated. Their structures and absolute configurations were elucidated by means of extensive spectroscopic data, including one- and two-dimensional nuclear magnetic resonance (NMR), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) analyses, and calculations of electronic circular dichroism (ECD). A chemical study on the solid-cultured fungus LF5 resulted in 11 polyketide derivatives, which included three previously undescribed derivatives: aspilactonol I (4), 2-(1-hydroxyethyl)-6-methylisonicotinic acid (7), and 6,8-dihydroxy-3-(1'R, 2'R-dihydroxypropyl)-isocoumarin (9), and two new natural-source-derived aspilactonols (G, H) (2, 3). Moreover, the absolute configuration of de-O-methyldiaporthin (11) was identified for the first time. Compounds 4 and 11 exhibited inhibitory activity against AChE with half maximal inhibitory concentration (IC(50)) values of 6.26 and 21.18 microM, respectively. Aspilactonol I (4) is the first reported furanone AChE inhibitor (AChEI). The results indicated that Phaeosphaeria is a good source of polyketide derivatives. This study identified intriguing lead compounds for further research and development of new AChEIs.
ESTHER : Xiao_2022_J.Fungi.(Basel)_8_
PubMedSearch : Xiao_2022_J.Fungi.(Basel)_8_
PubMedID: 35330234

Title : Anti-Ovarian cancer potential, in silico studies and anti-alzheimer's disease effects of some natural compounds as cholinesterase inhibitors - Fu_2022_Biotechnol.Appl.Biochem__
Author(s) : Fu M , Ji C , Yang T , Mao F , Shati AA , El-Kott AF , El-Maksoud MMA , Negm S , Ji Y
Ref : Biotechnol Appl Biochem , : , 2022
Abstract : Ovarian cancer ranks seventh in the most common malignant tumors in females and seriously threatens women's reproductive health. Natural sources may lead to basic research on potential bioactive components as lead compounds in drug discovery and ultimately therapeutic treatments for ovarian cancer and other diseases. sAlzheimer's disease and ovarian cancer are complex diseases of aging that impose an enormous public health burden worldwide.s Additionally, People with Alzheimer's disease have low levels of acetylcholine in their brain. Enzymes called cholinesterases break down acetylcholine in the brain. If their action is inhibited, more acetylcholine is available for communication between brain cells. In this study, pregnanolone, diethylstilbestrol, flavokawain C, methyl 3,4,5-trihydroxybenzoate molecules obtained excellent to good inhibitory against acetylcholinesterase and butyrylcholinesterase enzymes with IC(50) values ranging between 77.18+/-8.62 to 461.35+/-28.54 microM for acetylcholinesterase and 23.86+/-4.07 to 306.62+/-32.46 microM for butyrylcholinesterase. The calculations revealed the probable interactions and their characteristics at an atomic level. Indeed, the docking scores of diethylstilbestrol, flavokawain C, pregnanolone, and methyl 3,4,5-trihydroxybenzoate for AChE are -6.685, -6.247, -6.672, and -5.183 (kcal/mol), respectively. This value for the compounds against BuChE is -6.042, -8.851, -5.655, and -5.898 (kcal/mol), respectively. Additionally, these compounds significantly decreased ovarian cancer cell viability. Additionally, 100 microM dose of all molecules caused good reductions in ovarian cancer cell viability. This article is protected by copyright. All rights reserved.
ESTHER : Fu_2022_Biotechnol.Appl.Biochem__
PubMedSearch : Fu_2022_Biotechnol.Appl.Biochem__
PubMedID: 36515452

Title : A novel electrochemical sensing platform based on the esterase extracted from kidney bean for high-sensitivity determination of organophosphorus pesticides - Tao_2022_RSC.Adv_12_5265
Author(s) : Tao H , Liu F , Ji C , Wu Y , Wang X , Shi Q
Ref : RSC Adv , 12 :5265 , 2022
Abstract : Similar to acetylcholinesterase, the activity of plant-derived esterase can also be inhibited by organophosphorus pesticides. Therefore, an electrochemical sensing platform using kidney bean esterase as a new detection enzyme was proposed for the highly sensitive determination of organophosphorus pesticides. Purified kidney bean esterase was obtained by an efficient and economical aqueous two-phase extraction method. Carboxylated graphene/carbon nanotube composites (cCNTs-cGR) and Au nanoparticles were used to provide a biocompatible environment to immobilize kidney bean esterase and also accelerate electron transport between the analyte and the electrode surface. Due to the good synergistic electrocatalytic effects of these nanomaterials, the biosensor exhibited an amplified electrocatalytic response to the oxidation of alpha-naphthalenol, which makes the sensor more sensitive. Based on the inhibitory effect of trichlorfon on kidney bean esterase activity, high sensitivity and low-cost detection of trichlorfon was achieved. Under optimum conditions, the inhibition of trichlorfon is proportional to its concentration in the range of 5 to 150 ng L(-1) and 150 ng L(-1) to 700 ng L(-1) with an ultra-low detection limit of 3 ng L(-1). Moreover, the validity of the prepared biosensor was verified by analyzing several actual agricultural products (cabbage and rice) with satisfactory recoveries ranging from 94.05% to 106.76%, indicating that kidney bean esterase is a promising enzyme source for the analysis of organophosphorus pesticides in food samples.
ESTHER : Tao_2022_RSC.Adv_12_5265
PubMedSearch : Tao_2022_RSC.Adv_12_5265
PubMedID: 35425578

Title : Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses - Yin_2022_J.Hazard.Mater_434_128824
Author(s) : Yin C , Sun Z , Ji C , Li F , Wu H
Ref : J Hazard Mater , 434 :128824 , 2022
Abstract : As a typical organophosphorus pollutant, tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been widely detected in aquatic environment. Previous studies showed that protein phosphorylation might be a vital way of TDCIPP to exert multiple toxic effects. However, there is a lack of high-throughput investigations on how TDCIPP affected protein phosphorylation. In this study, the toxicological effects of TDCIPP were explored by proteomic and phosphoproteomic analyses together with traditional means in oysters Crassostrea gigas treated with 0.5, 5 and 50 microg/L TDCIPP for 28 days. Integration of omic analyses revealed that TDCIPP dysregulated transcription, energy metabolism, and apoptosis and cell proliferation by either directly phosphorylating pivotal proteins or phosphorylating their upstream signaling pathways. The U-shaped response of acetylcholinesterase activities suggested the neurotoxicity of TDCIPP in a hormesis manner. What's more, the increase in caspase-9 activity as well as the expression or phosphorylation alterations in eukaryotic translation initiation factor 4E, cell division control protein 42 and transforming growth factor-beta1-induced protein indicated the disruption of homeostasis between apoptosis and cell proliferation, which was consistent with the observation of shedding of digestive cells. Overall, combination of proteomic and phosphoproteomic analyses showed the capability of identifying molecular events, which provided new insights into the toxicological mechanisms of TDCIPP.
ESTHER : Yin_2022_J.Hazard.Mater_434_128824
PubMedSearch : Yin_2022_J.Hazard.Mater_434_128824
PubMedID: 35427976

Title : Old pesticide, new use: Smart and safe enantiomer of isocarbophos in locust control - Kong_2021_Ecotoxicol.Environ.Saf_225_112710
Author(s) : Kong Y , Ji C , Qu J , Chen Y , Wu S , Zhu X , Niu L , Zhao M
Ref : Ecotoxicology & Environmental Safety , 225 :112710 , 2021
Abstract : Locust plagues are still worldwide problems. Selecting active enantiomers from current chiral insecticides is necessary for controlling locusts and mitigating the pesticide pollution in agricultural lands. Herein, two enantiomers of isocarbophos (ICP) were separated and the enantioselectivity in insecticidal activity against the pest Locusta migratoria manilensis (L. migratoria) and mechanisms were investigated. The significant difference of LD(50) between (+)-ICP (0.609 mg/kg bw) and (-)-ICP (79.412 mg/kg bw) demonstrated that (+)-ICP was a more effective enantiomer. The enantioselectivity in insecticidal activity of ICP enantiomers could be attributed to the selective affinity to acetylcholinesterase (AChE). Results of in vivo and in vitro assays suggested that AChE was more sensitive to (+)-ICP. In addition, molecular docking showed that the -CDOKER energies of (+)-ICP and (-)-ICP were 25.6652 and 24.4169, respectively, which suggested a stronger affinity between (+)-ICP and AChE. Significant selectivity also occurred in detoxifying enzymes activities (carboxylesterases (CarEs) and glutathione S-transferases (GSTs)) and related gene expressions. Suppression of detoxifying enzymes activities with (+)-ICP treatment suggested that (-)-ICP may induce the detoxifying enzyme-mediated ICP resistance. A more comprehensive understanding of the enantioselectivity of ICP is necessary for improving regulation and risk assessment of ICP.
ESTHER : Kong_2021_Ecotoxicol.Environ.Saf_225_112710
PubMedSearch : Kong_2021_Ecotoxicol.Environ.Saf_225_112710
PubMedID: 34481357

Title : Enzyme-Triggered Disassembly of Perylene Monoimide-based Nanoclusters for Activatable and Deep Photodynamic Therapy - Cai_2020_Angew.Chem.Int.Ed.Engl__
Author(s) : Cai Y , Ni D , Cheng W , Ji C , Liu Y , Su Z , Chen C , Yin M , Mullen K , Wang Y
Ref : Angew Chem Int Ed Engl , : , 2020
Abstract : Photodynamic therapy (PDT) exhibits great potential for cancer therapy, but still suffers from nonspecific photosensitivity and poor penetration of photosensitizer. Here, a smart perylene monoimide-based nanocluster with enzyme-triggered disassembly is reported as an activatable and deeply penetrable photosensitizer. A novel carboxylesterase (CE)-responsive tetrachloroperylene monoimide (P1) was synthesized and assembled with folate-decorated albumins into a nanocluster ( FHP ) with a diameter of ~100 nm. Once P1 is hydrolyzed by the tumor-specific CE, FHP disassembles into ultrasmall nanoparticles (~10 nm), facilitating the deep tumor penetration of FHP . Furthermore, such enzyme-triggered disassembly of FHP leads to enhanced fluorescence intensity (~8-fold) and elevated singlet oxygen generation ability (~4-fold), enabling in situ near-infrared fluorescence imaging and promoted PDT. FHP permits remarkable tumor inhibition in vivo with minimal side effects through imaging-guided, activatable, and deep PDT. This work confirms that this cascaded multifunctional control via enzyme-triggered molecular disassembly is an effective strategy for precise cancer theranostics.
ESTHER : Cai_2020_Angew.Chem.Int.Ed.Engl__
PubMedSearch : Cai_2020_Angew.Chem.Int.Ed.Engl__
PubMedID: 32363672

Title : Characterization and Genome Analysis of a Zearalenone-Degrading Bacillus velezensis Strain ANSB01E - Guo_2020_Curr.Microbiol_77_273
Author(s) : Guo Y , Zhou J , Tang Y , Ma Q , Zhang J , Ji C , Zhao L
Ref : Curr Microbiol , 77 :273 , 2020
Abstract : Zearalenone, a nonsteroidal estrogenic mycotoxin mainly produced by Fusarium species, causes reproductive disorders and hyperestrogenic syndromes in animals and humans. The bacterial strain Bacillus velezensis ANSB01E, isolated from chicken cecal content, was capable of effectively degrading zearalenone in both liquid medium and mouldy corn. Moreover, Bacillus velezensis ANSB01E exhibited good antimicrobial activities against animal pathogenic bacteria, including Escherichia coli, Staphylococcus aureus, and Salmonella spp. Genome-based analysis revealed the presence of genes coding peroxiredoxin and alpha/beta hydrolase in Bacillus velezensis ANSB01E, which may be involved in zearalenone degradation. The study on the genome provides insights into the zearalenone degradation mechanisms and advances the potential application of Bacillus velezensis ANSB01E in food and feed industry.
ESTHER : Guo_2020_Curr.Microbiol_77_273
PubMedSearch : Guo_2020_Curr.Microbiol_77_273
PubMedID: 31748861
Gene_locus related to this paper: 9baci-QBK11187

Title : Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice - Ma_2019_Int.J.Biol.Macromol_133_1090
Author(s) : Ma H , Xiong H , Zhu X , Ji C , Xue J , Li R , Ge B , Cui H
Ref : Int J Biol Macromol , 133 :1090 , 2019
Abstract : The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice. Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology. The composition of intestinal microbiota was determined by genome sequencing analysis of the 16S rDNA. This study found that the average molecular weight of PSP was 29, 600Da, and mainly monosaccharides of PSP were rhamnose (24.7%), glucose (16.15%) and galactose (13.32%). The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice. In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea. The present s uncovered a new function of PSP, indicating that PSP could be used in constipation therapies.
ESTHER : Ma_2019_Int.J.Biol.Macromol_133_1090
PubMedSearch : Ma_2019_Int.J.Biol.Macromol_133_1090
PubMedID: 31054300

Title : Structural insights of a hormone sensitive lipase homologue Est22 - Huang_2016_Sci.Rep_6_28550
Author(s) : Huang J , Huo YY , Ji R , Kuang S , Ji C , Xu XW , Li J
Ref : Sci Rep , 6 :28550 , 2016
Abstract : Hormone sensitive lipase (HSL) catalyzes the hydrolysis of triacylglycerols into fatty acids and glycerol, thus playing key roles in energy homeostasis. However, the application of HSL serving as a pharmaceutical target and an industrial biocatalyst is largely hampered due to the lack of high-resolution structural information. Here we report biochemical properties and crystal structures of a novel HSL homologue esterase Est22 from a deep-sea metagenomic library. Est22 prefers short acyl chain esters and has a very high activity with substrate p-nitrophenyl butyrate. The crystal structures of wild type and mutated Est22 with its product p-nitrophenol are solved with resolutions ranging from 1.4 A to 2.43 A. The Est22 exhibits a alpha/beta-hydrolase fold consisting with a catalytic domain and a substrate-recognizing cap domain. Residues Ser188, Asp287, and His317 comprise the catalytic triad in the catalytic domain. The p-nitrophenol molecule occupies the substrate binding pocket and forms hydrogen bonds with adjacent residues Gly108, Gly109, and Gly189. Est22 exhibits a dimeric form in solution, whereas mutants D287A and H317A change to polymeric form, which totally abolished its enzymatic activities. Our study provides insights into the catalytic mechanism of HSL family esterase and facilitates the understanding for further industrial and biotechnological applications of esterases.
ESTHER : Huang_2016_Sci.Rep_6_28550
PubMedSearch : Huang_2016_Sci.Rep_6_28550
PubMedID: 27328716
Gene_locus related to this paper: 9bact-H6BDX1

Title : An unannotated alpha\/beta hydrolase superfamily member, ABHD6 differentially expressed among cancer cell lines - Li_2009_Mol.Biol.Rep_36_691
Author(s) : Li F , Fei X , Xu J , Ji C
Ref : Mol Biol Rep , 36 :691 , 2009
Abstract : Abhydrolase domain containing (Abhd) gene was a small group belongs to alpha/beta hydrolase superfamily. Known members of this group are all found to be involved in important biochemical processes and related to various diseases. In this paper, we report the tissue distribution, subcellular location and differential distribution among cancer cell lines of Abhd6, one unannotated member of this group.
ESTHER : Li_2009_Mol.Biol.Rep_36_691
PubMedSearch : Li_2009_Mol.Biol.Rep_36_691
PubMedID: 18360779
Gene_locus related to this paper: human-ABHD6

Title : Expression of lipoprotein lipase associated with lung adenocarcinoma tissues - Lu_2008_Mol.Biol.Rep_35_59
Author(s) : Lu J , Li J , Ji C , Yu W , Xu Z , Huang S
Ref : Mol Biol Rep , 35 :59 , 2008
Abstract : Lipoprotein lipase (LPL) plays a key role in the lipid metabolism and transporting. It can catalyze the hydrolysis of chylomicron and very low-density lipoprotein triglyceride. Moreover, the abnormality of LPL associates with many pathophysiological conditions. Herein cDNA microarray and Northern blots analysis were used to study the expression of lipoprotein lipase in lung adenocarcinoma tissues. There were 113 genes of all tested blots in cDNA microarray expressed lowly. LPL gene is expressed lowly at the average ratio 0.26 (Cy5/Cy3) in lung adenocarcinoma tissues over controls. Northern blots confirmed those changes detected from the cDNA microarray and suggested that low expression of LPL may play an important role in the lung adenocarcinoma development.
ESTHER : Lu_2008_Mol.Biol.Rep_35_59
PubMedSearch : Lu_2008_Mol.Biol.Rep_35_59
PubMedID: 17347923

Title : Effect of manganese supplementation and source on carcass traits, meat quality, and lipid oxidation in broilers - Lu_2007_J.Anim.Sci_85_812
Author(s) : Lu L , Luo XG , Ji C , Liu B , Yu SX
Ref : J Anim Sci , 85 :812 , 2007
Abstract : An experiment was conducted using a total of 336 one-day-old, Arbor Acres commercial male broilers to investigate the effect of dietary Mn supplementation on carcass traits, meat quality, lipid oxidation, relative enzyme activities in abdominal fat and meat, and Mn-containing superoxide dismutase (MnSOD) mRNA level in meat. Broilers were randomly allotted by BW to 1 of 8 replicate cages (6 chicks per cage) for each of 7 treatments in a completely randomized design involving a 2 x 3 factorial + 1 arrangement of treatments. Dietary treatments included the corn-soybean meal-based diet (control) and the basal diet supplemented with 100 or 200 mg of Mn/kg as MnSO(4) x H(2)O, Mn AA A with a chelation strength of 26.3 formation quotient (8.34% Mn), or Mn AA B with a chelation strength of 45.3 formation quotient (6.48% Mn). Birds fed supplemental Mn had lower (P < 0.10) percentages of abdominal fat, lipoprotein lipase (LPL), and malate dehydrogenase activities and greater (P < 0.07) hormone-sensitive lipase activities in abdominal fat than birds fed a control diet. Birds fed supplemental Mn from Mn AA A or Mn AA B had lower (P < 0.05) LPL activities in abdominal fat than those fed supplemental MnSO(4) x H(2)O. Birds fed supplemental Mn had lower (P < 0.03) malondialdehyde content in leg muscle and greater (P < 0.02) MnSOD activities and MnSOD mRNA level in breast or leg muscle than those fed the control diet. Birds fed supplemental Mn from Mn AA A had a greater (P < 0.02) MnSOD mRNA level in leg muscle than those fed supplemental MnSO(4) x H(2)O. Results from this study indicated that organic Mn was more available than inorganic Mn for decreasing LPL activity in abdominal fat of broilers, and dietary Mn might reduce abdominal adipose deposition by decreasing LPL and malate dehydrogenase activities or increasing hormone-sensitive lipase activity in abdominal adipose tissue. The results also indicated that dietary Mn upregulated muscle MnSOD gene expression pretranslationally in association with increased MnSOD activity, which might explain the decrease of malondialdehyde content in leg muscle.
ESTHER : Lu_2007_J.Anim.Sci_85_812
PubMedSearch : Lu_2007_J.Anim.Sci_85_812
PubMedID: 17040939