Ma H

References (30)

Title : Isolation, characteristics, and poly(butylene adipate-co-terephthalate) (PBAT) degradation mechanism of a marine bacteria Roseibium aggregatum ZY-1 - Pan_2024_Mar.Pollut.Bull_201_116261
Author(s) : Pan H , Yu T , Zheng Y , Ma H , Shan J , Yi X , Liu Y , Zhan J , Wang W , Zhou H
Ref : Mar Pollut Bull , 201 :116261 , 2024
Abstract : Marine microorganisms have been reported to degrade microplastics. However, the degradation mechanisms are still poorly understood. In this study, a bacterium Roseibium aggregatum ZY-1 was isolated from seawater, which can degrade poly(butylene adipate-co-terephthalate) (PBAT). The PBAT-PLA(polylactic acid, PLA) films, before and after degradation, were characterized by scanning electron microscope (SEM) and Fourier transform infrared spectrometer (FTIR), the weight loss rate and water contact angle were measured. The results indicate that ZY-1 colonized on PBAT-PLA film, changed the functional groups and decreased water contact angle of PBAT-PLA film. Moreover, liquid chromatography mass spectrometry (LC-MS) analysis reveales that PBAT was degraded into its oligomers (TB, BTB) and monomers (T, A) during 10 days, and adipic acid (A) could be used as a sole carbon source. The whole genome sequencing analyses illustrate the mechanisms and enzymes such as PETase, carboxylesterases, arylesterase (PpEst) and genes like pobA, pcaBCDFGHIJKT, dcaAEIJK, paaGHJ involved in PBAT degradation. Therefore, the R. aggregatum ZY-1 will be a promising candidate of PBAT degradation.
ESTHER : Pan_2024_Mar.Pollut.Bull_201_116261
PubMedSearch : Pan_2024_Mar.Pollut.Bull_201_116261
PubMedID: 38537567

Title : Frequencies of insecticide resistance mutations detected by the amplicon sequencing in Plutella xylostella (Lepidoptera: Plutellidae) and Spodoptera exigua (Lepidoptera: Noctuidae) from China - Liu_2024_J.Econ.Entomol__
Author(s) : Liu Z , Ma H , Li K , Liu J , Zhu H , Zhou Y , Man Y , Zhou X
Ref : J Econ Entomol , : , 2024
Abstract : The globally prevalent pests, Diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) and Beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae), pose significant threats to cruciferous vegetables. They have rapidly developed resistance to a wide range of insecticides, leading to significant yield losses and increased control expenses. In this study, we have established an efficient approach utilizing amplicon sequencing to detect the frequency of 15 target resistance mutant sites in 6 molecular targets, acetylcholinesterase 1 (ACE1), chitin synthase 1 (CHS1), the gamma-aminobutyric acid receptor (GABAR), glutamate-gated chloride channel (GluCl), voltage-gated sodium channels (NaV), and ryanodine receptor (RyR) in P. xylostella and the frequency of 11 mutations in 5 molecular targets (except GluCl) in S. exigua in China. Our findings indicate that P. xylostella exhibits remarkably high frequency (over 88.67%) in pyrethroid resistance-related mutations T929I and L1014F of NaV. In S. exigua, the frequencies of L659F mutation were ranging from 41.92% to 74.89%. In addition, the organophosphorus resistance-related mutations A298S and G324A of ACE1 were detected at frequencies ranging from 34.29% to 75.66%, and these 2 mutations occurred simultaneously (from 29.22% to 65.79%) in P. xylostella. An interannual variation in mutation frequency from 2019 to 2021 was found for P. xylostella in HNCS. The frequency of A298S and G324A mutations steadily increased while the frequency of G4946E and I4790M mutations continuously decreased. These results unveil a worrisome scenario of multiple resistance sites in these 2 pests in China and provide valuable insights for the practical application of pesticides in the field.
ESTHER : Liu_2024_J.Econ.Entomol__
PubMedSearch : Liu_2024_J.Econ.Entomol__
PubMedID: 38748551

Title : Changes in the VOC of Fruits at Different Refrigeration Stages of 'Ruixue' and the Participation of Carboxylesterase MdCXE20 in the Catabolism of Volatile Esters - Li_2023_Foods_12_1977
Author(s) : Li D , Guo J , Ma H , Pei L , Liu X , Wang H , Chen R , Zhao Z , Gao H
Ref : Foods , 12 :1977 , 2023
Abstract : Aroma is a crucial quality attribute of apple fruit, which significantly impacts its commercial value and consumer choice. Despite its importance the volatile aroma substances produced by the new variety 'Ruixue' after harvest remain unclear. In this study, we utilized headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) to investigate the changes in volatile substances, fruit hardness, crispness, and related aroma synthase activity of commercially mature 'Ruixue' apples during cold storage. Our findings revealed a gradual decline in fruit firmness and brittleness of 'Ruixue' apples during cold storage, with hexyl acetate, hexyl caproate, and hexyl thiocyanate being the main hexyl esters detected. To gain a better understanding of the metabolic pathway of esters, we identified 42 MdCXE gene members that are associated with ester degradation. Through RT-qPCR analysis, we discovered that carboxylesterase MdCXE20 exhibited higher expression levels compared to other MdCXE genes during cold storage. To confirm the role of MdCXE20, we conducted a transient injection of apple fruits and observed that overexpression of MdCXE20 led to the degradation of esters such as hexyl hexanoate, butyl hexanoate, butyl 2-methylbutyrate, hexyl butyrate, and hexyl 2-methylbutyrate. The results of the study showed that the virus-induced gene silencing of MdCXE20 found the opposite results. Additionally, the esters of OE-MdCXE20 callus showed a lower content of ester VOC than the control callus, according to the homologous stable transformation of 'Wanglin' callus. Overall, these findings suggest that the MdCXE20 gene plays a crucial role in the decrease of esters in 'Ruixue' apples, which ultimately affects their flavor.
ESTHER : Li_2023_Foods_12_1977
PubMedSearch : Li_2023_Foods_12_1977
PubMedID: 37238795

Title : Harnessing extremophilic carboxylesterases for applications in polyester depolymerisation and plastic waste recycling - Williams_2023_Essays.Biochem__
Author(s) : Williams GB , Ma H , Khusnutdinova AN , Yakunin AF , Golyshin PN
Ref : Essays Biochem , : , 2023
Abstract : The steady growth in industrial production of synthetic plastics and their limited recycling have resulted in severe environmental pollution and contribute to global warming and oil depletion. Currently, there is an urgent need to develop efficient plastic recycling technologies to prevent further environmental pollution and recover chemical feedstocks for polymer re-synthesis and upcycling in a circular economy. Enzymatic depolymerization of synthetic polyesters by microbial carboxylesterases provides an attractive addition to existing mechanical and chemical recycling technologies due to enzyme specificity, low energy consumption, and mild reaction conditions. Carboxylesterases constitute a diverse group of serine-dependent hydrolases catalysing the cleavage and formation of ester bonds. However, the stability and hydrolytic activity of identified natural esterases towards synthetic polyesters are usually insufficient for applications in industrial polyester recycling. This necessitates further efforts on the discovery of robust enzymes, as well as protein engineering of natural enzymes for enhanced activity and stability. In this essay, we discuss the current knowledge of microbial carboxylesterases that degrade polyesters (polyesterases) with focus on polyethylene terephthalate (PET), which is one of the five major synthetic polymers. Then, we briefly review the recent progress in the discovery and protein engineering of microbial polyesterases, as well as developing enzyme cocktails and secreted protein expression for applications in the depolymerisation of polyester blends and mixed plastics. Future research aimed at the discovery of novel polyesterases from extreme environments and protein engineering for improved performance will aid developing efficient polyester recycling technologies for the circular plastics economy.
ESTHER : Williams_2023_Essays.Biochem__
PubMedSearch : Williams_2023_Essays.Biochem__
PubMedID: 37334661

Title : Complete Depolymerization of PET Wastes by an Evolved PET Hydrolase from Directed Evolution - Shi_2023_Angew.Chem.Int.Ed.Engl_62_e202218390
Author(s) : Shi L , Liu P , Tan Z , Zhao W , Gao J , Gu Q , Ma H , Liu H , Zhu L
Ref : Angew Chem Int Ed Engl , 62 :e202218390 , 2023
Abstract : PETase displays great potential in PET depolymerization. Directed evolution has been limited to engineer PETase due to the lack of high-throughput screening assay. In this study, a novel fluorescence-based high-throughput screening assay employing a newly designed substrate, bis (2-hydroxyethyl) 2-hydroxyterephthalate (termed BHET-OH), was developed for PET hydrolases. The best variant DepoPETase produced 1407-fold more products towards amorphous PET film at 50 degreesC and showed a 23.3 degreesC higher T(m) value than the PETase WT. DepoPETase enabled complete depolymerization of seven untreated PET wastes and 19.1g PET waste (0.4 % W(enzyme) /W(PET) ) in liter-scale reactor, suggesting that it is a potential candidate for industrial PET depolymerization processes. The molecular dynamic simulations revealed that the distal substitutions stabilized the loops around the active sites and transmitted the stabilization effect to the active sites through enhancing inter-loop interactions network.
ESTHER : Shi_2023_Angew.Chem.Int.Ed.Engl_62_e202218390
PubMedSearch : Shi_2023_Angew.Chem.Int.Ed.Engl_62_e202218390
PubMedID: 36751696
Gene_locus related to this paper: idesa-peth

Title : Inhibitory Effects of Cannabinoids on Acetylcholinesterase and Butyrylcholinesterase Enzyme Activities - Puopolo_2022_Med.Cannabis.Cannabinoids_5_85
Author(s) : Puopolo T , Liu C , Ma H , Seeram NP
Ref : Med Cannabis Cannabinoids , 5 :85 , 2022
Abstract : INTRODUCTION: Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are two cholinergic enzymes catalyzing the reaction of cleaving acetylcholine into acetate and choline at the neuromuscular junction. Abnormal hyperactivity of AChE and BChE can lead to cholinergic deficiency, which is associated with several neurological disorders including cognitive decline and memory impairments. Preclinical studies support that some cannabinoids including cannabidiol (CBD) and tetrahydrocannabinol (THC) may exert pharmacological effects on the cholinergic system, but it remains unclear whether cannabinoids can inhibit AChE and BChE activities. Herein, we aimed to evaluate the inhibitory effects of a panel of cannabinoids including CBD, delta8-THC, cannabigerol (CBG), cannabigerolic acid (CBGA), cannabicitran (CBT), cannabidivarin (CBDV), cannabichromene (CBC), and cannabinol (CBN) on AChE and BChE activities. METHODS: The inhibitory effects of cannabinoids on the activities of AChE and BChE enzymes were evaluated with the Ellman method using acetyl- and butyryl-thiocholines as substrates. The inhibition mechanism of cannabinoids on AChE and BChE was studied with enzyme kinetic assays including the Lineweaver-Burk and Michaelis-Menten analyses. In addition, computational-based molecular docking experiments were performed to explore the interactions between the cannabinoids and the enzyme proteins. RESULTS: Cannabinoids including CBD, delta8-THC, CBG, CBGA, CBT, CBDV, CBC, and CBN (at 200 microM) inhibited the activities of AChE and BChE by 70.8, 83.7, 92.9, 76.7, 66.0, 79.3, 13.7, and 30.5%, and by 86.8, 80.8, 93.2, 87.1, 77.0, 78.5, 27.9, and 22.0%, respectively. The inhibitory effects of these cannabinoids (with IC(50) values ranging from 85.2 to >200 microM for AChE and 107.1 to >200 microM for BChE) were less potent as compared to the positive control galantamine (IC(50) 1.21 and 6.86 microM for AChE and BChE, respectively). In addition, CBD, as a representative cannabinoid, displayed a competitive type of inhibition on both AChE and BChE. Data from the molecular docking studies suggested that cannabinoids interacted with several amino acid residues on the enzyme proteins, which supported their overall inhibitory effects on AChE and BChE. CONCLUSION: Cannabinoids showed moderate inhibitory effects on the activities of AChE and BChE enzymes, which may contribute to their modulatory effects on the cholinergic system. Further studies using cell-based and in vivo models are warranted to evaluate whether cannabinoids' neuroprotective effects are associated with their anti-cholinesterase activities.
ESTHER : Puopolo_2022_Med.Cannabis.Cannabinoids_5_85
PubMedSearch : Puopolo_2022_Med.Cannabis.Cannabinoids_5_85
PubMedID: 35702400

Title : New fluorescent probe with recognition moiety of bipiperidinyl reveals the rise of hepatocellular carboxylesterase activity during heat shock - Liu_2022_Biosens.Bioelectron_211_114392
Author(s) : Liu Y , He Z , Yang Y , Li X , Li Z , Ma H
Ref : Biosensors & Bioelectronics , 211 :114392 , 2022
Abstract : Heat shock is a heat-related pathology characterized by a high body temperature and an obvious change of many enzymatic activities. Carboxylesterase (CE), as the major hydrolase in liver, is responsible for the hydrolysis of many drugs or the detoxification of various toxins from all organs. However, the correlation between heat shock and the CE activity in cells remains unknown, mainly due to the lack of a suitable research approach. Herein, a new water-soluble fluorescence probe, MYO-CE, with a specific bipiperidinyl recognition moiety has been developed for detecting the CE activity. MYO-CE reacted selectively with CE instead of other esterase, causing a large fluorescence off-on response at 560 nm with a detection limit of 0.39 U/mL. The applicability of MYO-CE for cell imaging was demonstrated by monitoring the alteration of the hepatocellular CE activity under inflammation. More importantly, we investigated the change of the CE activity during heat shock, uncovering a significant increase for the first time. This finding was further validated by a commercial colorimetric kit assay. The proposed probe shows a promising prospect for the CE study in cells under different pathological conditions.
ESTHER : Liu_2022_Biosens.Bioelectron_211_114392
PubMedSearch : Liu_2022_Biosens.Bioelectron_211_114392
PubMedID: 35609457

Title : Characterization of the synergistic inhibitory effect of cyanidin-3-O-glucoside and catechin on pancreatic lipase - Wang_2022_Food.Chem_404_134672
Author(s) : Wang Y , Chen L , Liu H , Xie J , Yin W , Xu Z , Ma H , Wu W , Zheng M , Liu M , Liu J
Ref : Food Chem , 404 :134672 , 2022
Abstract : This study aimed to identify novel pancreatic lipase (PL) inhibitors using affinity ultrafiltration combined with spectroscopy and molecular docking. Cyanidin-3-O-glucoside (C3G; IC(50): 0.268 mg/mL) and catechin (IC(50): 0.280 mg/mL) were shown to be potent PL inhibitors extracted from black rice and adzuki bean coat extracts. Isobologram analysis revealed that the combined use of C3G and catechin at a ratio of 2:3 had a remarkable synergistic effect (IC(50) of the mixture: 0.201 mg/mL). The inhibitory mechanism of C3G-catechin mixture was of mixed type. The C3G-catechin mixture had a great impact on PL secondary structures. Molecular docking analysis further demonstrated that these polyphenols formed hydrophobic interactions and hydrogen bonds with amino acid residues in the binding pocket of PL. Collectively, C3G and catechin were shown to inhibit PL in a synergistic manner and can be potentially used for the development of food supplements for obesity prevention.
ESTHER : Wang_2022_Food.Chem_404_134672
PubMedSearch : Wang_2022_Food.Chem_404_134672
PubMedID: 36323025

Title : Thifluzamide exposure induced neuro-endocrine disrupting effects in zebrafish (Danio rerio) - Yang_2021_Arch.Toxicol__
Author(s) : Yang Y , Chang J , Wang D , Ma H , Li Y , Zheng Y
Ref : Archives of Toxicology , : , 2021
Abstract : Thifluzamide is widely used fungicide and frequently detected in aquatic system. In this study, the toxicity of fungicide thifluzamide to non-targeted aquatic organisms was investigated for neuroendocrine disruption potentials. Here, zebrafish embryos were exposed to a series of concentrations of thifluzamide for 6 days. The results showed that both the development of embryos/larvae and the behavior of hatched larvae were significantly affected by thifluzamide. Importantly, the decreased activity of acetylcholinesterase (AchE) and the increased contents of neurotransmitters such as serotonin (5-HT) and norepinephrine (NE), along with transcriptional changes of nervous system related genes were observed following 4 days exposure to thifluzamide. Besides, the decreased contents of triiodothyronine (T3) and thyroxine (T4) in whole body, as well as significant expression alteration in hypothalamic-pituitary-thyroid (HPT) axis associated genes were discovered in zebrafish embryos after 4 days of exposure to thifluzamide. Our results clearly demonstrated that zebrafish embryos exposed to thifluzamide could disrupt neuroendocrine, compromise behavior and induce developmental abnormality, suggesting impact of this fungicide on developmental programming in zebrafish.
ESTHER : Yang_2021_Arch.Toxicol__
PubMedSearch : Yang_2021_Arch.Toxicol__
PubMedID: 34635929

Title : Recent advances in multitarget-directed ligands targeting G-protein-coupled receptors - Ma_2020_Drug.Discov.Today_25_1682
Author(s) : Ma H , Huang B , Zhang Y
Ref : Drug Discov Today , 25 :1682 , 2020
Abstract : Mounting evidence indicates that single-target drugs might be inadequate to achieve satisfactory therapeutic effects on complex diseases. Recently, increasing attention has been paid to developing drugs that can manipulate multiple targets to generate beneficial effects through potential synergy. G-protein-coupled receptors (GPCRs) become desirable targets for developing multitarget-directed ligands (MTDLs) because of their crucial roles in the pathophysiology of various human diseases and the accessibility of druggable sites at the cell surface. Herein, we review the most recent advances in the development of GPCR-targeted MTDLs in treating complex diseases, and discuss their potential therapeutic strategies to reveal current trends and shed insights into the utility of GPCR-targeted MTDLs for future drug design and development.
ESTHER : Ma_2020_Drug.Discov.Today_25_1682
PubMedSearch : Ma_2020_Drug.Discov.Today_25_1682
PubMedID: 32652312

Title : A molecular approach to rationally constructing specific fluorogenic substrates for the detection of acetylcholinesterase activity in live cells, mice brains and tissues - Wu_2020_Chem.Sci_11_11285
Author(s) : Wu X , An JM , Shang J , Huh E , Qi S , Lee E , Li H , Kim G , Ma H , Oh MS , Kim D , Yoon J
Ref : Chem Sci , 11 :11285 , 2020
Abstract : Acetylcholinesterase (AChE) is an extremely critical hydrolase tightly associated with neurological diseases. Currently, developing specific substrates for imaging AChE activity still remains a great challenge due to the interference from butyrylcholinesterase (BChE) and carboxylesterase (CE). Herein, we propose an approach to designing specific substrates for AChE detection by combining dimethylcarbamate choline with a self-immolative scaffold. The representative P10 can effectively eliminate the interference from CE and BChE. The high specificity of P10 has been proved via imaging AChE activity in cells. Moreover, P10 can also be used to successfully map AChE activity in different regions of a normal mouse brain, which may provide important data for AChE evaluation in clinical studies. Such a rational and effective approach can also provide a solid basis for designing probes with different properties to study AChE in biosystems and another way to design specific substrates for other enzymes.
ESTHER : Wu_2020_Chem.Sci_11_11285
PubMedSearch : Wu_2020_Chem.Sci_11_11285
PubMedID: 34094370

Title : Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice - Ma_2019_Int.J.Biol.Macromol_133_1090
Author(s) : Ma H , Xiong H , Zhu X , Ji C , Xue J , Li R , Ge B , Cui H
Ref : Int J Biol Macromol , 133 :1090 , 2019
Abstract : The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice. Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology. The composition of intestinal microbiota was determined by genome sequencing analysis of the 16S rDNA. This study found that the average molecular weight of PSP was 29, 600Da, and mainly monosaccharides of PSP were rhamnose (24.7%), glucose (16.15%) and galactose (13.32%). The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice. In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea. The present s uncovered a new function of PSP, indicating that PSP could be used in constipation therapies.
ESTHER : Ma_2019_Int.J.Biol.Macromol_133_1090
PubMedSearch : Ma_2019_Int.J.Biol.Macromol_133_1090
PubMedID: 31054300

Title : The Neuropeptide Y System Regulates Both Mechanical and Histaminergic Itch - Gao_2018_J.Invest.Dermatol_138_2405
Author(s) : Gao T , Ma H , Xu B , Bergman J , Larhammar D , Lagerstrom MC
Ref : Journal of Investigative Dermatology , 138 :2405 , 2018
Abstract : Itch is a somatosensory modality that serves to alert an organism to harmful elements removable by scratching, such as parasites and chemical irritants. Recently, ablation or silencing of neuropeptide Y (NPY)-expressing spinal interneurons was reported to selectively enhance mechanical itch, whereas chemical itch was unaffected. We examined the effect of activating the NPY/Y1 receptor system on scratch behavior in mice. We found that intrathecal administration of the Y1 agonist [Leu(31),Pro(34)]-NPY (LP-NPY) attenuated itch behavior induced by application of 0.07 g von Frey filament in the nape of the neck compared with saline treatment, indicating that activation of the spinal NPY/Y1 system dampens mechanical itch. However, intrathecal administration of LP-NPY also attenuated chemically induced scratching provoked by intradermal application of histamine or the mast cell degranulator 48/80 (histaminergic itch), and the latter effect could be reversed by administration of the Y1 antagonist BIBO3304. Intrathecal application of the native nonselective agonist NPY also attenuated histamine or 48/80-induced scratching. Our analyses emphasize the importance of including additional quantitative parameters to characterize the full spectrum of itch behavior and show that the NPY/Y1 system dampens both mechanically and chemically induced scratching and hence is shared by the two submodalities of itch.
ESTHER : Gao_2018_J.Invest.Dermatol_138_2405
PubMedSearch : Gao_2018_J.Invest.Dermatol_138_2405
PubMedID: 29803641

Title : Development of a neuroprotective potential algorithm for medicinal plants - Liu_2016_Neurochem.Int_100_164
Author(s) : Liu W , Ma H , DaSilva NA , Rose KN , Johnson SL , Zhang L , Wan C , Dain JA , Seeram NP
Ref : Neurochem Int , 100 :164 , 2016
Abstract : Medicinal plants are promising candidates for Alzheimer's disease (AD) research but there is lack of systematic algorithms and procedures to guide their selection and evaluation. Herein, we developed a Neuroprotective Potential Algorithm (NPA) by evaluating twenty-three standardized and chemically characterized Ayurvedic medicinal plant extracts in a panel of bioassays targeting oxidative stress, carbonyl stress, protein glycation, amyloid beta (Abeta) fibrillation, acetylcholinesterase (AChE) inhibition, and neuroinflammation. The twenty-three herbal extracts were initially evaluated for: 1) total polyphenol content (Folin-Ciocalteu assay), 2) free radical scavenging capacity (DPPH assay), 3) ferric reducing antioxidant power (FRAP assay), 4) reactive carbonyl species scavenging capacity (methylglyoxal trapping assay), 5) anti-glycative effects (BSA-fructose, and BSA-methylglyoxal assays) and, 6) anti-Abeta fibrillation effects (thioflavin-T assay). Based on assigned index scores from the initial screening, twelve extracts with a cumulative NPA score >/=40 were selected for further evaluation for their: 1) inhibitory effects on AChE activity, 2) in vitro anti-inflammatory effects on murine BV-2 microglial cells (Griess assay measuring levels of lipopolysaccharide-induced nitric oxide species), and 3) in vivo neuroprotective effects on Caenorhabditis elegans post induction of Abeta1-42 induced neurotoxicity and paralysis. Among these, four extracts had a cumulative NPA score >/=60 including Phyllanthus emblica (amla; Indian gooseberry), Mucuna pruriens (velvet bean), Punica granatum (pomegranate) and Curcuma longa (turmeric; curcumin). These extracts also showed protective effects on H2O2 induced cytotoxicity in differentiated cholinergic human neuronal SH-SY5Y and murine BV-2 microglial cells and reduced tau protein levels in the SH-SY5Y neuronal cells. While published animal data support the neuroprotective effects of several of these Ayurvedic medicinal plant extracts, some remain unexplored for their anti-AD potential. Therefore, the NPA may be utilized, in part, as a strategy to help guide the selection of promising medicinal plant candidates for future AD-based research using animal models.
ESTHER : Liu_2016_Neurochem.Int_100_164
PubMedSearch : Liu_2016_Neurochem.Int_100_164
PubMedID: 27693453

Title : Correction to Obtaining Optical Purity for Product Diols in Enzyme-Catalyzed Epoxide Hydrolysis: Contributions from Changes in both Enantio- and Regioselectivity -
Author(s) : Janfalk Carlsson A , Bauer P , Ma H , Widersten M
Ref : Biochemistry , 55 :1940 , 2016
PubMedID: 26986896

Title : Ultrasensitive Fluorescent Probes Reveal an Adverse Action of Dipeptide Peptidase IV and Fibroblast Activation Protein during Proliferation of Cancer Cells - Gong_2016_Anal.Chem_88_8309
Author(s) : Gong Q , Shi W , Li L , Wu X , Ma H
Ref : Analytical Chemistry , 88 :8309 , 2016
Abstract : Dipeptide peptidase IV (DPPIV) and fibroblast activation protein (FAP) are isoenzymes. Evidence shows that DPPIV is related to antitumor immunity, and FAP may be a drug target in cancer therapy, making it seem that the two enzymes might have a synergistic role during the proliferation of cancer cells. Surprisingly, herein, we find an adverse action of DPPIV and FAP in the proliferation process by analyzing their changes with two tailor-made ultrasensitive fluorescent probes. First, the up-regulation of DPPIV and down-regulation of FAP in cancer cells under the stimulation of genistein are detected. Then, we find that MGC803 cells with a higher FAP but lower DPPIV level than SGC7901 cells exhibit a faster proliferation rate. Importantly, inhibiting the DPPIV expression with siRNA increases the proliferation rate of MGC803 cells, whereas the FAP inhibition decreases the rate. These findings suggest that the two enzymes play an adverse role during the proliferation of cancer cells, which provides us a new viewpoint for cancer studies.
ESTHER : Gong_2016_Anal.Chem_88_8309
PubMedSearch : Gong_2016_Anal.Chem_88_8309
PubMedID: 27444320

Title : The neurovascular protective effects of huperzine a on d-galactose-induced inflammatory damage in the rat hippocampus - Ruan_2014_Gerontology_60_424
Author(s) : Ruan Q , Hu X , Ao H , Ma H , Gao Z , Liu F , Kong D , Bao Z , Yu Z
Ref : Gerontology , 60 :424 , 2014
Abstract : BACKGROUND: Chronic administration of D-galactose (D-gal) results in oxidative stress and chronic inflammatory aging. Age-related changes in the brain result in neurovascular damage and blood-brain barrier (BBB) dysfunction. However, little is known regarding D-gal-induced neurovascular damage, as well as the protective effects of huperzine A. OBJECTIVE: The purpose of this study was to utilize a D-gal-induced rat model to investigate the activation of neurovascular inflammatory damage and apoptosis in the rat hippocampus and to understand whether huperzine A alleviates D-gal-induced neuronal and vascular inflammatory injury.
METHODS: Aging rats were treated with D-gal (300 mg/kg s.c. for 8 weeks), were coadministered D-gal and huperzine A (D-gal 300 mg/kg and huperzine A 0.1 mg/kg s.c. for 8 weeks) or served as the saline-treated control group rats (same volume of saline given subcutaneously for 8 weeks). Changes in hippocampal morphology and biomarkers of inflammatory damage were analyzed.
RESULTS: Our study revealed that chronic administration of D-gal resulted in the activation of glia and vascular endothelial cells and upregulation of mRNA and protein levels of cell-associated adhesion molecules and inflammatory cytokines via nuclear factor (NF)-kappaB inhibitor degradation and NF-kappaB nuclear translocation. The inflammatory injury caused significant BBB dysfunction, decreased density of tight junctions (TJs) and apoptosis in the rat hippocampus. Coadministration of huperzine A not only markedly inhibited the D-gal-induced increase in acetylcholinesterase (AChE) activity, but also alleviated D-gal-induced neurovascular damage by inhibiting D-gal-induced NF-kappaB activation, improving cerebrovascular function and suppressing the D-gal-induced decrease in the density and protein levels of TJs and cell apoptosis.
CONCLUSIONS: Our findings provided evidence that D-gal induced a proinflammatory phenotype mediated by NF-kappaB in the rat hippocampus. Moreover, huperzine A suppressed D-gal-induced neurovascular damage and BBB dysfunction, partly by preventing NF-kappaB nuclear translocation. The inhibiting effect of huperzine A on AChE activity might play an important role in attenuating D-gal-induced inflammatory damage. (c) 2014 S. Karger AG, Basel.
ESTHER : Ruan_2014_Gerontology_60_424
PubMedSearch : Ruan_2014_Gerontology_60_424
PubMedID: 24969491

Title : Disease-modifying effects of RHC80267 and JZL184 in a pilocarpine mouse model of temporal lobe epilepsy - Ma_2014_CNS.Neurosci.Ther_20_905
Author(s) : Ma L , Wang L , Yang F , Meng XD , Wu C , Ma H , Jiang W
Ref : CNS Neurosci Ther , 20 :905 , 2014
Abstract : INTRODUCTION: Patients with temporal lobe epilepsy (TLE) often suffer from comorbid psychiatric diagnoses such as depression, anxiety, or impaired cognitive performance. Endocannabinoid (eCB) signaling is a key regulator of synaptic neurotransmission and has been implicated in the mechanisms of epilepsy as well as several mood disorders and cognitive impairments. AIMS: We employed a pilocarpine model of TLE in C57/BJ mice to investigate the role of eCB signaling in epileptogenesis and concomitant psychiatric comorbidities. METHODS AND
RESULTS: We sought to alter the neuronal levels of a known eCB receptor ligand, 2-arachidonylglycerol (2-AG), through the use of RHC80267 or JZL184. Pilocarpine-treated mice were treated with RHC80267 (1.3 mumol) or JZL184 (20 mg/kg) immediately after the termination of status epilepticus (SE), which was followed by daily treatment for the next 7 days. Our results indicated that RHC80267 treatment significantly reduced the percentage of mice suffering from spontaneous recurrent seizures (SRS) in addition to decreasing the duration of observed seizures when compared to vehicle treatment. Furthermore, RHC80267 attenuated depression and anxiety-related behaviors, improved previously impaired spatial learning and memory, and inhibited seizure-induced hippocampal neuronal loss during the chronic epileptic period. In contrast, JZL184 administration markedly increased the frequency and the duration of observed SRS, enhanced the previously impaired neuropsychological performance, and increased hippocampal damage following SE.
CONCLUSIONS: These findings suggest that RHC80267 treatment after the onset of SE could result in an amelioration of the effects found during the chronic epileptic period and yield an overall decrease in epileptic symptoms and comorbid conditions. Thus, alterations to endocannabinoid signaling may serve as a potential mechanism to prevent epileptogenesis and manipulation of this signaling pathway as a possible drug target.
ESTHER : Ma_2014_CNS.Neurosci.Ther_20_905
PubMedSearch : Ma_2014_CNS.Neurosci.Ther_20_905
PubMedID: 24989980

Title : DWARF 53 acts as a repressor of strigolactone signalling in rice - Jiang_2013_Nature_504_401
Author(s) : Jiang L , Liu X , Xiong G , Liu H , Chen F , Wang L , Meng X , Liu G , Yu H , Yuan Y , Yi W , Zhao L , Ma H , He Y , Wu Z , Melcher K , Qian Q , Xu HE , Wang Y , Li J
Ref : Nature , 504 :401 , 2013
Abstract : Strigolactones (SLs) are a group of newly identified plant hormones that control plant shoot branching. SL signalling requires the hormone-dependent interaction of DWARF 14 (D14), a probable candidate SL receptor, with DWARF 3 (D3), an F-box component of the Skp-Cullin-F-box (SCF) E3 ubiquitin ligase complex. Here we report the characterization of a dominant SL-insensitive rice (Oryza sativa) mutant dwarf 53 (d53) and the cloning of D53, which encodes a substrate of the SCF(D3) ubiquitination complex and functions as a repressor of SL signalling. Treatments with GR24, a synthetic SL analogue, cause D53 degradation via the proteasome in a manner that requires D14 and the SCF(D3) ubiquitin ligase, whereas the dominant form of D53 is resistant to SL-mediated degradation. Moreover, D53 can interact with transcriptional co-repressors known as TOPLESS-RELATED PROTEINS. Our results suggest a model of SL signalling that involves SL-dependent degradation of the D53 repressor mediated by the D14-D3 complex.
ESTHER : Jiang_2013_Nature_504_401
PubMedSearch : Jiang_2013_Nature_504_401
PubMedID: 24336200

Title : [A noninvasive diagnostic model of liver fibrosis using serum markers in primary biliary cirrhosis] - Ma_2012_Zhonghua.Nei.Ke.Za.Zhi_51_618
Author(s) : Ma JL , Wang R , Zhang FK , Jia JD , Ou XJ , Zhang T , Wang Y , Duan WJ , Zhao XY , You H , Ma H
Ref : Zhonghua Nei Ke Za Zhi , 51 :618 , 2012
Abstract : OBJECTIVE To verify and assess diagnostic value of noninvasive diagnostic model of liver fibrosis in primary biliary cirrhosis PBC based on conventional laboratory markers METHODS Seventy-three patients with PBC diagnosed by liver biopsy between January 2003 and June 2011 in Beijing Friendship Hospital Capital Medical University were recruited in this study Correlation analysis and logistic regression analysis between the conventional laboratory markers and histology stages were assessed A liver fibrosis diagnostic model was established based upon aforementioned biomarkers and verified by its sensitivity and specificity for predicting the liver fibrosis RESULTS The predictive model H index consisting of five conventional laboratory markers i.e platelet count serum cholinesterase albumin HDL-C and prothrombin time activity could predict advanced fibrosis stages III-IV with an AUC(ROC of 0.861 The sensitivity of predicting the absence of advanced fibrosis using H index 2.20 was 96.6 and the specificity of predicting the presence of advanced fibrosis using H index 0.41 was 93.2 CONCLUSION The established noninvasive diagnostic model consisting of five laboratory markers could accurately distinguish pathological changes of early stage PBC stages I-II from advanced stage PBC stages III-IV).
ESTHER : Ma_2012_Zhonghua.Nei.Ke.Za.Zhi_51_618
PubMedSearch : Ma_2012_Zhonghua.Nei.Ke.Za.Zhi_51_618
PubMedID: 23158860

Title : The yak genome and adaptation to life at high altitude - Qiu_2012_Nat.Genet_44_946
Author(s) : Qiu Q , Zhang G , Ma T , Qian W , Wang J , Ye Z , Cao C , Hu Q , Kim J , Larkin DM , Auvil L , Capitanu B , Ma J , Lewin HA , Qian X , Lang Y , Zhou R , Wang L , Wang K , Xia J , Liao S , Pan S , Lu X , Hou H , Wang Y , Zang X , Yin Y , Ma H , Zhang J , Wang Z , Zhang Y , Zhang D , Yonezawa T , Hasegawa M , Zhong Y , Liu W , Huang Z , Zhang S , Long R , Yang H , Lenstra JA , Cooper DN , Wu Y , Shi P , Liu J
Ref : Nat Genet , 44 :946 , 2012
Abstract : Domestic yaks (Bos grunniens) provide meat and other necessities for Tibetans living at high altitude on the Qinghai-Tibetan Plateau and in adjacent regions. Comparison between yak and the closely related low-altitude cattle (Bos taurus) is informative in studying animal adaptation to high altitude. Here, we present the draft genome sequence of a female domestic yak generated using Illumina-based technology at 65-fold coverage. Genomic comparisons between yak and cattle identify an expansion in yak of gene families related to sensory perception and energy metabolism, as well as an enrichment of protein domains involved in sensing the extracellular environment and hypoxic stress. Positively selected and rapidly evolving genes in the yak lineage are also found to be significantly enriched in functional categories and pathways related to hypoxia and nutrition metabolism. These findings may have important implications for understanding adaptation to high altitude in other animal species and for hypoxia-related diseases in humans.
ESTHER : Qiu_2012_Nat.Genet_44_946
PubMedSearch : Qiu_2012_Nat.Genet_44_946
PubMedID: 22751099
Gene_locus related to this paper: bosmu-l8ic43 , bovin-2neur , bovin-balip , bovin-BCHE , bovin-e1bbv2 , bovin-e1bn79 , bovin-est8 , bovin-f1mi11 , bovin-f1n385 , bovin-g3mxp5 , bovin-lipli , bovin-lipr2 , bovin-q2kj30 , bovin-q3sz79 , bovin-q3t0r6 , bovin-ABHDA , bovin-q08dw9 , bovin-ABHD16B , bovin-SPG21 , bovin-TEX30 , 9ceta-l8iwv2 , 9ceta-l8idy3 , 9ceta-l8hsi3 , bovin-e1bjq9 , bovin-f1mc21 , 9ceta-l8hyl8 , bovin-LIPG , bovin-a0a3q1nm09 , bovin-f1n2i5

Title : A spectroscopic off-on probe for simple and sensitive detection of carboxylesterase activity and its application to cell imaging - Zhang_2012_Analyst_137_716
Author(s) : Zhang Y , Chen W , Feng D , Shi W , Li X , Ma H
Ref : Analyst , 137 :716 , 2012
Abstract : A new resorufin-based spectroscopic probe, 7-(p-acetoxyphenylmethyloxy)-3H-phenoxazin-3-one (1), has been developed for detecting carboxylesterase activity. The probe is designed by introducing p-acetoxyphenylmethyloxy as a bifunctional moiety to resorufin. The p-acetoxyphenylmethyloxy moiety not only quenches the spectroscopic signal of resorufin, but also serves as a recognition unit for carboxylesterase. As a result, the prepared latent spectroscopic probe 1 shows very low background signal, which is rather desired for achieving sensitive detection. The specific cleavage of the carboxylic ester bond by carboxylesterase induces the hydrolysis of the probe, resulting in the release of resorufin and thereby the recovery of both color and fluorescence signal. This behaviour leads to the development of a simple and sensitive fluorescent method for assaying carboxylesterase activity, with a detection limit of 8.6 x 10(-5) U mL(-1), which is much more sensitive than the existing fluorescence approaches. Moreover, experimental results showed that the probe 1 is cell membrane permeable, and its applicability has been demonstrated for monitoring carboxylesterase activity in HeLa cells.
ESTHER : Zhang_2012_Analyst_137_716
PubMedSearch : Zhang_2012_Analyst_137_716
PubMedID: 22159212

Title : Hyper-phosphorylation of GSK-3beta: Possible roles in chlorpyrifos-induced behavioral alterations in animal model of depression - Chen_2012_Neurosci.Lett_528_148
Author(s) : Chen WQ , Ma H , Bian JM , Zhang YZ , Li J
Ref : Neuroscience Letters , 528 :148 , 2012
Abstract : In recent years, the widespread use of chlorpyrifos (CPF) has aroused concerns regarding its potential neurotoxic effects, especially in developing individuals. One of the major consequences of CPF exposure is mood disorders such as depression. Epidemiological studies have demonstrated susceptibility to depression in populations with a history of CPF exposure. Our previous study indicated that repeated CPF exposure in doses from 10 to 160mg/kg elicits depression- and anxiety-like alterations. However, whether this alteration is due to persistent inhibition of acetylcholinesterase (AChE) was not determined. In this study, we used lower doses of CPF to avoid evident inhibition of AChE to investigate other potential target systems that contribute to CPF's neurotoxic effect. Four-week-old adolescent male rats were repeatedly exposed to CPF at doses of 2.5, 5, or 10mg/kg (s.c., 10 days) and then were subjected to either neurobehavioral testing or immunoblot analysis. Depression-like behaviors as manifested by increased immobility time was observed in force swimming test, while immunoblot analysis revealed a dramatically increased phosphorylation of glycogen synthase kinase-3beta (GSK-3beta) in the hippocampus and striatum, with no effect on the levels of Wnt2, GSK-3beta, or beta-catenin. These results suggest a noncholinergic mechanism, the hyper-phosphorylation of GSK-3beta, which may contribute to the cellular neurotoxicity of CPF, thus increasing the susceptibility to mood disorders.
ESTHER : Chen_2012_Neurosci.Lett_528_148
PubMedSearch : Chen_2012_Neurosci.Lett_528_148
PubMedID: 22985519

Title : Crystal structure of a soluble form of human monoglyceride lipase in complex with an inhibitor at 1.35 A resolution - Schalk-Hihi_2011_Protein.Sci_20_670
Author(s) : Schalk-Hihi C , Schubert C , Alexander R , Bayoumy S , Clemente JC , Deckman I , Desjarlais RL , Dzordzorme KC , Flores CM , Grasberger B , Kranz JK , Lewandowski F , Liu L , Ma H , Maguire D , Macielag MJ , McDonnell ME , Mezzasalma Haarlander T , Miller R , Milligan C , Reynolds C , Kuo LC
Ref : Protein Science , 20 :670 , 2011
Abstract : A high-resolution structure of a ligand-bound, soluble form of human monoglyceride lipase (MGL) is presented. The structure highlights a novel conformation of the regulatory lid-domain present in the lipase family as well as the binding mode of a pharmaceutically relevant reversible inhibitor. Analysis of the structure lacking the inhibitor indicates that the closed conformation can accommodate the native substrate 2-arachidonoyl glycerol. A model is proposed in which MGL undergoes conformational and electrostatic changes during the catalytic cycle ultimately resulting in its dissociation from the membrane upon completion of the cycle. In addition, the study outlines a successful approach to transform membrane associated proteins, which tend to aggregate upon purification, into a monomeric and soluble form.
ESTHER : Schalk-Hihi_2011_Protein.Sci_20_670
PubMedSearch : Schalk-Hihi_2011_Protein.Sci_20_670
PubMedID: 21308848
Gene_locus related to this paper: human-MGLL

Title : Hepatic lipolysis in broiler chickens with different fat deposition during embryonic development - Zhao_2010_Res.Vet.Sci_88_321
Author(s) : Zhao S , Ma H , Huang G , Zou S
Ref : Res Vet Sci , 88 :321 , 2010
Abstract : The aim of this study was to explore the hepatic lipolysis in broiler chickens with different fat deposition during embryonic development. The mRNA expression of CPT-1 (carmitine palmtoyltransferase-1), PPARalpha (peroxisome proliferator-activated receptor alpha) and LPL (lipoprotein lipase) genes were determined using Real time RT-PCR. The start of incubation was called day 1 (E1) and after hatching called day 1 (H1). On incubation days 9 (E9), 14 (E14) and 19 (E19) as well as at hatching (H1), samples of liver were collected. Blood samples were obtained during days 14 (E14) and 19 (E19) of embryonic development and at hatching. This study showed that serum TG (triglycerol) decreased and TC (total cholesterol) and NEFA (non-estered fatty acid) increased during embryonic development. The expression of CPT-1, PPARalpha and LPL genes exhibited different developmental changes. For example, little LPL gene was expressed at hatching and PPARalpha gene expression peaked before hatching. However, CPT-1 gene exhibited no significance during the embryonic development. Our results showed that expression of these genes in Arbor Acres (AA) broilers was significantly higher than that in San Huang (SH) broilers. Therefore, this study suggested that hepatic lipolysis in broiler chickens exhibited developmental changes during embryogenesis and breed difference which may be one of the factors in the fat deposition difference between fat line and lean line broilers during embryonic development.
ESTHER : Zhao_2010_Res.Vet.Sci_88_321
PubMedSearch : Zhao_2010_Res.Vet.Sci_88_321
PubMedID: 19709700

Title : Acetylcholine receptor gamma-subunits mRNA isoforms expressed in denervated rat muscle - Li_2008_Mol.Neurobiol_37_164
Author(s) : Li AM , Ma H , Villarroel A
Ref : Molecular Neurobiology , 37 :164 , 2008
Abstract : The fetal acetylcholine (ACh) receptor, composed of the alphabetagammadelta subunits, is expressed in fetal, neonatal, and denervated muscle. Single-channel recording has revealed three kinetically distinct classes in neonatal and denervated muscle, suggesting that at least three forms of the gamma-subunit are required. To account for the kinetic classes observed, we compared the messenger ribonucleic acid (mRNA) forms expressed in neonatal and denervated muscle using reverse transcriptase polymerase chain reaction, cloning, and RNAse protection assays. We found five novel forms arising from alternative splicing, which we named gamma5-gamma9. The forms gamma5, gamma6, and gamma7 lack exon 4 and 63-, 89-, and 136 bp of exon 5, respectively. A gamma8 form lacks exons 3 and 4 and 19 bp of exon 5. The last, gamma9, lacks exons 3, 4, and 5. Results indicate that gamma4 predominates in fetal muscle and gamma7 in denervated adult muscle. Some of the gamma-subunit mRNAs found may generate the receptors observed in muscle.
ESTHER : Li_2008_Mol.Neurobiol_37_164
PubMedSearch : Li_2008_Mol.Neurobiol_37_164
PubMedID: 18548353

Title : Prolonged effects of poly(lactic-co-glycolic acid) microsphere-containing huperzine A on mouse memory dysfunction induced by scopolamine - Wang_2007_Basic.Clin.Pharmacol.Toxicol_100_190
Author(s) : Wang C , Zhang T , Ma H , Liu J , Fu F , Liu K
Ref : Basic Clin Pharmacol Toxicol , 100 :190 , 2007
Abstract : Huperzine A is an anticholinesterase and cognitive enhancer, which is able to alleviate the symptoms of memory dysfunction in the mouse. The fast metabolization rate and narrow therapeutic spectrum makes it unfit for clinical use. Poly(lactic-co-glycolic acid) microsphere as delivery system effectively maintains the blood concentration of huperzine A by a slow-release effect over a long time. In the present article, we investigated the prolonged protective effect of microsphere-containing huperzine A on memory dysfunction induced by scopolamine. Spectrophotometric assay was used to determine the activity of acetylcholinesterase (AChE) and passive avoidance tests to evaluate memory performance. The results show that a bolus dose of microsphere-containing huperzine A (at a dose of 300 microg/kg or 600 microg/kg) administered intramuscularly can effectively maintain drug activity and significantly decrease the activity of AChE from day 3 to 14, the strongest effect seen on day 3 and 7. Accompanying the reduction of the activity of AChE, microsphere-containing huperzine A (300 microg/kg or 600 microg/kg) remarkably increased transfer latency time and no transfer response on the second trial through mitigating the memory impairments induced by scopolamine as compared to the scopolamine model group. Microsphere-containing huperzine A showed cognitive enhancing properties and anticholinesterase activity and may thus be a candidate for treatment of memory impairment.
ESTHER : Wang_2007_Basic.Clin.Pharmacol.Toxicol_100_190
PubMedSearch : Wang_2007_Basic.Clin.Pharmacol.Toxicol_100_190
PubMedID: 17309523

Title : Effect of surface hydrophobicity\/hydrophilicity of mesoporous supports on the activity of immobilized lipase - He_2006_J.Colloid.Interface.Sci_298_780
Author(s) : He J , Xu Y , Ma H , Zhang Q , Evans DG , Duan X
Ref : J Colloid Interface Sci , 298 :780 , 2006
Abstract : Taking advantage of the virtue of hydrophilic surface, lipase was firstly immobilized on SBA-15 as a support. Then the surface of the SBA-15 with enzyme entrapped inside the channels was modified by grafting with organic moieties. It has been found that the silylation with n-decyltrimethoxysilane (DE) and 3-(trimethoxysilyl)propyl methacrylate (MA) following the lipase immobilization increases the surface hydrophobicity. But the surface modified by MA shows more hydrophilicity than that modified by DE. The activity assay indicates that the hydrolytic activity for the hydrolysis of insoluble or partly soluble substrates increases with enhanced surface hydrophobicity.
ESTHER : He_2006_J.Colloid.Interface.Sci_298_780
PubMedSearch : He_2006_J.Colloid.Interface.Sci_298_780
PubMedID: 16430912

Title : [The value of coagulation factors in severity categorization of hepatitis B-related liver cirrhosis] - Li_2005_Zhonghua.Nei.Ke.Za.Zhi_44_188
Author(s) : Li Q , Wang BE , Cong YL , Jia JD , Yin ZJ , Qian LX , Ma H
Ref : Zhonghua Nei Ke Za Zhi , 44 :188 , 2005
Abstract : OBJECTIVE: To investigate the diagnostic value of coagulation factors in assessing the severity degree of liver cirrhosis caused by hepatitis B.
METHODS: Fifty-eight patients with liver cirrhosis and twenty healthy persons as control were enrolled. Prothrombin time activity percentage (PTA), activated partial thromboplastin time, coagulation activity of factor II, V, VII, VIII, IX and X were detected with clotting assay. Antithrombin-III (AT-III) was detected with colorimetric assay. The biochemical markers were also detected.
RESULTS: The differences of PTA, factor II, VII and AT-III among Child-Pugh A, B, C in patients with liver cirrhosis were statistically significant (P < 0.01). Through receiver operating characteristic curve analysis, when 64% and 50% were used as cut-off values for PTA and factor VII in diagnosing Child-Pugh B, the area under the curve (AUC) was 0.689 and 0.610, the sensitivity was 76.9% and 61.5%, the specificity was 62.2% and 55.6%; when 54% and 39% were used as cut-off values for PTA and factor VII in diagnosing Child-Pugh C, the AUC was 0.924 and 0.942, the sensitivity was 80.0% and 86.7%, the specificity was 88.4% and 90.7%. Stepwise linear regression was done between Child-Pugh grade and coagulation factors. PTA, cholinesterase (Che), total bilirubin (TBil), albumin (Alb), factor VII were included in regression equation, Y = 15.008 - 0.018 x PTA - 0.288 x Che + 0.264 x TBil - 0.988 x Alb - 0.034 x VII, R(2) = 0.871. Patients whose Y was less than 8 were classified as grade "a", between 8 - 10 as grade "b", more than 10 as grade "c", the diagnostic accuracy was 84.5%. CONCLUSION: Coagulation factor VII may serve as a helpful marker in diagnosing the severity degree of liver cirrhosis.
ESTHER : Li_2005_Zhonghua.Nei.Ke.Za.Zhi_44_188
PubMedSearch : Li_2005_Zhonghua.Nei.Ke.Za.Zhi_44_188
PubMedID: 15840257

Title : A clinical analysis of 104 cases of acute pure and mixed organophosphate poisoning - Zhang_2002_Zhonghua.Nei.Ke.Za.Zhi_41_544
Author(s) : Zhang J , Zhao J , Sun S , Ma H , Zhao C , Guo Z , Wang F
Ref : Zhonghua Nei Ke Za Zhi , 41 :544 , 2002
Abstract : OBJECTIVE To study the difference of the clinical manifestations between single and mixed acute organophosphate (OP) poisoning. METHODS: The clinical signs and symptoms, the activity of cholinesterase (ChE) in erythrocytes, plasma and whole blood, and the level of AST, CK, LDH and ALT were compared between a single OP poisoning group (Group S) and a mixed OP poisoning group (Group C). RESULTS: Group S and Group C compare with: (1) Symptoms and signs on arrival at hospital: Group C was found to have more cases showing, nausea and vomiting than group S with obvious difference (P < 0.05). (2) The rates of other symptoms and signs were of no significant difference between the 2 groups. The activity of cholinesterase of the 2 groups on arrival at hospital: Whole blood ChE < 0.30: 16 cases and 14 cases; > 0.30 approximately : 24 cases and 19 cases; 0.50 approximately 0.70: 14 cases and 17 cases; erythrocyte ChE < 0.30: 18 cases and 14 cases; > 0.30 approximately : 22 cases and 21 cases; 0.50 approximately 0.70: 14 cases and 15 cases; plasma ChE < 0.30: 28 cases and 25 cases; > 0.30 approximately : 10 cases and 12 cases; 0.50 approximately 0.70: 16 cases and 13 cases; chi(2) = 0.852, 1.444, 0.509. There was no obvious difference (P > 0.05). (3) Positive rates of serum biochemical parameters between the 2 groups within 72 hours after arrival at hospital: Group S AST 24 cases, ALT 18 cases, CK 42 cases, LDH 22 cases, Tbil 21 cases; Group C AST 20 cases, ALT 11 cases, CK 32 cases, LDH 18 cases, Tbil 17 cases. There was also no obvious difference (P > 0.05). (4) Positive rate of ECG: between the 2 group on arrival at hospital Group S 24 cases, Group C 19 cases. No obvious difference was shown (P > 0.05). (5) Fatality rates between the 2 groups: Group S 7.41% (4/54), Group C 6.00% (3/50), chi(2) = 0.082, P > 0.05. CONCLUSION: Acute mixed OP poisoning and single OP poisoning show no significant difference in clinical manifestations. The treatment measures for single OP poisoning also has good effect fo mixed OP poisoning.
ESTHER : Zhang_2002_Zhonghua.Nei.Ke.Za.Zhi_41_544
PubMedSearch : Zhang_2002_Zhonghua.Nei.Ke.Za.Zhi_41_544
PubMedID: 12421504