Liao Y

References (23)

Title : PLA2G7\/PAF-AH as Potential Negative Regulator of the Wnt Signaling Pathway Mediates Protective Effects in BRCA1 Mutant Breast Cancer - Liao_2023_Int.J.Mol.Sci_24_882
Author(s) : Liao Y , Badmann S , Kraus F , Topalov NE , Mayr D , Kolben T , Hester A , Beyer S , Mahner S , Jeschke U , Trillsch F , Czogalla B , Burges A
Ref : Int J Mol Sci , 24 : , 2023
Abstract : Past studies have confirmed that aberrant activation of the Wnt/beta-catenin signaling is associated with tumorigenesis and metastasis in breast cancer, while the role of platelet-activating factor acetylhydrolase (PLA2G7/PAF-AH) in this signaling pathway remains unclear. In this study, we analyze the functional impact of PAF-AH on BRCA1 mutant breast cancer and explore its relationship to the Wnt signaling pathway. By performing immunohistochemistry, PAF-AH expression and beta-catenin expression were examined in both BRCA1 WT and BRCA1 mutant breast cancer specimens. The BRCA1 mutant breast cancer cell line HCC1937 was used for in vitro experiments to assess the impact of PAF-AH on cellular functions. The intracellular distribution of beta-catenin depending on PLA2G7/PAF-AH expression was investigated by immunocytochemistry. Significantly higher nuclear expression levels of PAF-AH were found in BRCA1 mutant tissue specimens than in BRCA1 WT samples. Cell viability, proliferation, and the motility rate of HCC1937 were significantly enhanced after PLA2G7 silencing, which indicated a protective role of PAF-AH in breast cancer. Nuclear PAF-AH expressed correlatedly with membranous beta-catenin. PLA2G7 silencing provoked the beta-catenin translocation from the membrane to the nucleus and activated Wnt signaling downstream genes. Our data showed a protective effect of high PAF-AH expression in BRCA1 mutant breast cancer. PAF-AH may achieve its protective effect by negatively regulating the Wnt pathway. In conclusion, our research sheds new light on the regulatory pathways in BRCA1 mutant breast cancer.
ESTHER : Liao_2023_Int.J.Mol.Sci_24_882
PubMedSearch : Liao_2023_Int.J.Mol.Sci_24_882
PubMedID: 36614323
Gene_locus related to this paper: human-PLA2G7

Title : Optimal dose of neostigmine antagonizing cisatracurium-induced shallow neuromuscular block in elderly patients: a randomized control study - Cao_2023_BMC.Anesthesiol_23_269
Author(s) : Cao M , Huang H , Tong J , Ou Y , Liao Y
Ref : BMC Anesthesiol , 23 :269 , 2023
Abstract : BACKGROUND: Residual neuromuscular block after using neuromuscular blocking agents is a common and potentially harmful complication of general anesthesia. Neostigmine is a widely used antagonist, but its optimal dose for elderly patients is unclear. OBJECTIVES: To compare the optimal dosage and safety of neostigmine for reversing shallow residual block in elderly patients after cisatracurium-induced neuromuscular block. METHODS: A randomized controlled trial was conducted in 196 elderly patients undergoing non-cardiac surgery under general anesthesia with cisatracurium. Patients were assigned to receive either no neostigmine (control group) or neostigmine at 20 microg/kg, 40 microg/kg or 50 microg/kg when train-of-four (TOF) ratio reached 0.2 at the end of surgery. The primary outcome was the time to reach TOF ratio of 0.9 after administration. Secondary outcomes included TOF ratio at 10 min after administration, postoperative nausea and vomiting, postoperative cognitive impairment and post-anesthesia care unit (PACU) stay time. RESULTS: The time to reach TOF ratio of 0.9 in the 20 microg/kg, 40 microg/kg and 50 microg/kg groups was significantly shorter than the control group (H = 104.257, P < 0.01), and the time of 40 microg/kg group and 50 microg/kg group was significantly shorter than the 20 microg/kg group (P < 0.001). There was no significant difference between 40 microg/kg and 50 microg/kg groups (P = 0.249). The TOF ratio at 10 min after administration showed similar results. There were no significant differences among groups in postoperative nausea and vomiting, postoperative cognitive impairment or post-operation hospital stay. CONCLUSIONS: Timely use of neostigmine after general anesthesia in elderly patients can significantly shorten time of TOF value reaching 0.9, among which 40 microg/kg dosage may be a more optimized choice. TRIAL REGISTRATION: this study was registered on chictr.org.cn (ChiCTR2100054685, 24/12/2021).
ESTHER : Cao_2023_BMC.Anesthesiol_23_269
PubMedSearch : Cao_2023_BMC.Anesthesiol_23_269
PubMedID: 37563623

Title : Inhibition mechanism of fisetin on acetylcholinesterase and its synergistic effect with galantamine - Shi_2023_Spectrochim.Acta.A.Mol.Biomol.Spectrosc_305_123452
Author(s) : Shi W , Han W , Liao Y , Wen J , Zhang G
Ref : Spectrochim Acta A Mol Biomol Spectrosc , 305 :123452 , 2023
Abstract : The search for acetylcholinesterase (AChE) inhibitors produced by natural sources is of great significance for the prevention and therapy of Alzheimer's disease and has been widely concerned. In this study, fisetin, a flavonoid compound of plant origin, displayed a mixed inhibition mode on AChE (IC(50) = 8.88 +/- 0.14 microM). Fluorescence spectra analysis revealed that fisetin statically quenched AChE fluorescence, and the ground state complex was formed by hydrogen bonds and hydrophobic interactions. Circular dichroism assays showed that fisetin induced AChE structure loosened with a decrease in alpha-helix structure (from 20.6 % to 19.5 %). Computer simulation exhibited that fisetin bound to both the peripheral anionic site (PAS) and the catalytic active site (CAS) and increased the stability of the AChE. Interestingly, the combination of fisetin and galantamine enhanced the binding affinity between AChE and galantamine and induced AChE structure further loosened, while the inhibition mode was still the mixed type. The heatmap analysis indicated that galantamine (0.2 microM) combined with fisetin (2.25 microM) had a significant synergy on AChE inhibition, probably because fisetin binding at the PAS-AChE induced conformation changes of the gorge and CAS, which enhanced galantamine binding affinity with CAS, and a further loose structure of AChE was induced by the mixture, so finally the interaction between the substrate and AChE was strongly affected. This work may offer a theoretical reference for the functional research of fisetin as a potential AChE inhibitor and an enhanced supplement for galantamine.
ESTHER : Shi_2023_Spectrochim.Acta.A.Mol.Biomol.Spectrosc_305_123452
PubMedSearch : Shi_2023_Spectrochim.Acta.A.Mol.Biomol.Spectrosc_305_123452
PubMedID: 37769468

Title : FASN promotes lymph node metastasis in cervical cancer via cholesterol reprogramming and lymphangiogenesis - Du_2022_Cell.Death.Dis_13_488
Author(s) : Du Q , Liu P , Zhang C , Liu T , Wang W , Shang C , Wu J , Liao Y , Chen Y , Huang J , Tan H , Zhao Y , Xia M , Liu J , Yao S
Ref : Cell Death Dis , 13 :488 , 2022
Abstract : Cervical cancer (CC) patients with lymph node metastasis (LNM) have a poor prognosis. Clarification of the detailed mechanisms underlying LNM may provide potential clinical therapeutic targets for CC patients with LNM. However, the molecular mechanism of LNM in CC is unclear. In the present study, we demonstrated that fatty acid synthase (FASN), one of the key enzymes in lipid metabolism, had upregulated expression in the CC samples and was correlated with LNM. Moreover, multivariate Cox proportional hazards analysis identified FASN as an independent prognostic factor of CC patients. Furthermore, gain-of-function and loss-of-function approaches showed that FASN promoted CC cell migration, invasion, and lymphangiogenesis. Mechanistically, on the one hand, FASN could regulate cholesterol reprogramming and then activate the lipid raft-related c-Src/AKT/FAK signaling pathway, leading to enhanced cell migration and invasion. On the other hand, FASN induced lymphangiogenesis by secreting PDGF-AA/IGFBP3. More importantly, knockdown of FASN with FASN shRNA or the inhibitors C75 and Cerulenin dramatically diminished LNM in vivo, suggesting that FASN plays an essential role in LNM of CC and the clinical application potential of FASN inhibitors. Taken together, our findings uncover a novel molecular mechanism in LNM of CC and identify FASN as a novel prognostic factor and potential therapeutic target for LNM in CC.
ESTHER : Du_2022_Cell.Death.Dis_13_488
PubMedSearch : Du_2022_Cell.Death.Dis_13_488
PubMedID: 35597782

Title : Exploring the Inhibition of Quercetin on Acetylcholinesterase by Multispectroscopic and In Silico Approaches and Evaluation of Its Neuroprotective Effects on PC12 Cells - Liao_2022_Molecules_27_
Author(s) : Liao Y , Mai X , Wu X , Hu X , Luo X , Zhang G
Ref : Molecules , 27 : , 2022
Abstract : This study investigated the inhibitory mechanism of quercetin in acetylcholinesterase (AChE) and its neuroprotective effects on beta-amyloid(25-35)-induced oxidative stress injury in PC12 cells. Quercetin inhibited AChE in a reversible mixed manner with an IC(50) of 4.59 +/- 0.27 microM. The binding constant of quercetin with AChE at 25 degreesC was (5.52 +/- 0.05) x 10(4) L mol(-1). Hydrogen bonding and van der Waals forces were the main interactions in forming the stable quercetin-AChE complex. Computational docking revealed that quercetin was dominant at the peripheral aromatic site in AChE and induced enzymatic allosterism; meanwhile, it extended deep into the active center of AChE and destabilized the hydrogen bond network, which caused the constriction of the gorge entrance and prevented the substrate from entering the enzyme, thus resulting in the inhibition of AChE. Molecular dynamics (MD) simulation emphasized the stability of the quercetin-AChE complex and corroborated the previous findings. Interestingly, a combination of galantamine hydrobromide and quercetin exhibited the synergistic inhibition effect by binding to different active sites of AChE. In a beta-amyloid(25-35)-induced oxidative stress injury model in PC12 cells, quercetin exerted neuroprotective effects by increasing the glutathione level and reducing the malondialdehyde content and reactive oxygen species levels. These findings may provide novel insights into the development and application of quercetin in the dietary treatment of Alzheimer's disease.
ESTHER : Liao_2022_Molecules_27_
PubMedSearch : Liao_2022_Molecules_27_
PubMedID: 36432070

Title : Inhibitory Mechanism of Baicalein on Acetylcholinesterase: Inhibitory Interaction, Conformational Change, and Computational Simulation - Liao_2022_Foods_11_
Author(s) : Liao Y , Hu X , Pan J , Zhang G
Ref : Foods , 11 : , 2022
Abstract : Alzheimer's disease (AD) is the most prevalent chronic neurodegenerative disease in elderly individuals, causing dementia. Acetylcholinesterase (AChE) is regarded as one of the most popular drug targets for AD. Herbal secondary metabolites are frequently cited as a major source of AChE inhibitors. In the current study, baicalein, a typical bioactive flavonoid, was found to inhibit AChE competitively, with an associated IC(50) value of 6.42 +/- 0.07 microM, through a monophasic kinetic process. The AChE fluorescence quenching by baicalein was a static process. The binding constant between baicalein and AChE was an order of magnitude of 10(4) L mol(-1), and hydrogen bonding and hydrophobic interaction were the major forces for forming the baicalein-AChE complex. Circular dichroism analysis revealed that baicalein caused the AChE structure to shrink and increased its surface hydrophobicity by increasing the alpha-helix and beta-turn contents and decreasing the beta-sheet and random coil structure content. Molecular docking revealed that baicalein predominated at the active site of AChE, likely tightening the gorge entrance and preventing the substrate from entering and binding with the enzyme, resulting in AChE inhibition. The preceding findings were confirmed by molecular dynamics simulation. The current study provides an insight into the molecular-level mechanism of baicalein interaction with AChE, which may offer new ideas for the research and development of anti-AD functional foods and drugs.
ESTHER : Liao_2022_Foods_11_
PubMedSearch : Liao_2022_Foods_11_
PubMedID: 35053900

Title : Design, Synthesis, and Evaluation of a Series of Novel Super Long-Acting DPP-4 Inhibitors for the Treatment of Type 2 Diabetes - Zhang_2020_J.Med.Chem_63_7108
Author(s) : Zhang C , Ye F , Wang J , He P , Lei M , Huang L , Huang A , Tang P , Lin H , Liao Y , Liang Y , Ni J , Yan P
Ref : Journal of Medicinal Chemistry , 63 :7108 , 2020
Abstract : In the present work, a novel series of trifluoromethyl-substituted tetrahydropyran derivatives were rationally designed and synthesized as potent DPP-4 inhibitors with significantly improved duration time of action over current commercially available DPP-4 inhibitors. The incorporation of the trifluoromethyl group on the 6-position of the tetrahydropyran ring of omarigliptin with the configuration of (2R,3S,5R,6S) not only significantly improves the overall pharmacokinetic profiles in mice but also maintains comparable DPP-4 inhibition activities. Further preclinical development of compound 2 exhibited its extraordinary efficacy in vivo and good safety profile. Clinical studies of compound 2 (Haisco HSK7653) are now ongoing in China, which revealed that inhibitor 2 could serve as an efficient candidate with a once-biweekly therapeutic regimen.
ESTHER : Zhang_2020_J.Med.Chem_63_7108
PubMedSearch : Zhang_2020_J.Med.Chem_63_7108
PubMedID: 32452679

Title : miR-140-3p Inhibits Cutaneous Melanoma Progression by Disrupting AKT\/p70S6K and JNK Pathways through ABHD2 - He_2020_Mol.Ther.Oncolytics_17_83
Author(s) : He Y , Yang Y , Liao Y , Xu J , Liu L , Li C , Xiong X
Ref : Mol Ther Oncolytics , 17 :83 , 2020
Abstract : Because cutaneous melanoma (CM) is one of the most lethal human tumors, major treatment advances are vital. miR-140-3p has been suggested to act as a suppressor in a range of malignant tumors, implying its possible use as a biomarker for effective antineoplastic treatment. However, the potential role of miR-140-3p in CM and the underlying mechanism remain unclear. In the present study, we identified lower levels of miR-140-3p in both CM tissues and cell lines; this downregulation was strongly associated with worse CM survival. Additionally, overexpression of miR-140-3p significantly inhibited cell proliferation, migration, and invasion in CM cells with different cell line origins. Importantly, by means of both bioinformatics analysis and luciferase reporter assay, we revealed abhydrolase domain containing 2 (ABHD2) to be a target of miR-140-3p in CM cells. Upregulation of ABHD2 reversed the tumor-suppressive effects of miR-140-3p in CM cells. Furthermore, miR-140-3p-targeted ABHD2 played a role in both activation of JNK signaling and inhibition of the AKT/p70S6K pathway in CM cells. Finally, in vivo results strongly suggested the suppressive effects of miR-140-3p on CM growth and metastasis. Collectively, our findings highlight a novel antineoplastic function for miR-140-3p in CM through ABHD2.
ESTHER : He_2020_Mol.Ther.Oncolytics_17_83
PubMedSearch : He_2020_Mol.Ther.Oncolytics_17_83
PubMedID: 32322665
Gene_locus related to this paper: human-ABHD2

Title : Aster glehni Extract Ameliorates Scopolamine-Induced Cognitive Impairment in Mice - Liao_2019_J.Med.Food_22_685
Author(s) : Liao Y , Bae HJ , Park JH , Zhang J , Koo B , Lim MK , Han EH , Lee SH , Jung SY , Lew JH , Ryu JH
Ref : J Med Food , 22 :685 , 2019
Abstract : The leaves of Aster glehni Fr. Schm. (Asteraceae) have been used to treat insomnia in Korea. Insomnia is a common adverse effect of therapeutic agents for Alzheimer's disease (AD), and the control of sleep disturbance may prevent dementia. We hypothesized that the leaves of A. glehni can attenuate cognitive dysfunctions observed in AD. We observed the ameliorating effects of the ethanolic extract of leaves of A. glehni (AG-D) on memory dysfunction through the Morris water maze test, the passive avoidance test, and the Y-maze test. We performed acetylcholinesterase (AChE) activity assay and Western blotting to determine the mechanism of action of AG-D. AG-D significantly attenuated memory dysfunction observed in the above behavior studies and inhibited the activity of AChE. AG-D also increased the levels of phosphorylation extracellular signal-regulated kinase (ERK), cAMP response element-binding protein (CREB), phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), and glycogen synthase kinase 3beta (GSK-3beta) and the expression levels of brain-derived neurotrophic factor (BDNF) in the hippocampi. These results suggest that AG-D ameliorates memory impairments by AChE inhibition and activation of ERK-CREB-BDNF and PI3K-Akt-GSK-3beta signaling pathways. Taken together, this study suggests that AG-D could be used as a potential treatment for cognitive dysfunction.
ESTHER : Liao_2019_J.Med.Food_22_685
PubMedSearch : Liao_2019_J.Med.Food_22_685
PubMedID: 31225769

Title : Ethanolic Extract of Opuntia ficus-indica var. saboten Ameliorates Cognitive Dysfunction Induced by Cholinergic Blockade in Mice - Kwon_2018_J.Med.Food_21_971
Author(s) : Kwon Y , Liao Y , Koo B , Bae H , Zhang J , Han EH , Yun SM , Lim MK , Lee SH , Jung SY , Ryu JH
Ref : J Med Food , 21 :971 , 2018
Abstract : The stem of Opuntia ficus-indica var. saboten is edible and has been used as a medicinal herb on Jeju Island in Korea. We previously reported that the butanolic extract of O. ficus-indica var. saboten exerts the enhancement of long-term memory in mice. However, the antiamnesic effects of O. ficus-indica var. saboten and its mode of action has not been clearly elucidated. In the present study, we explored the effects of the ethanolic extract of stems of O. ficus-indica var. saboten (EOFS) on cognitive performance in mouse and attempted to delineate its mechanism of action. We used the passive avoidance, Y-maze, and novel object recognition tests to assess its effects on cognitive functions in scopolamine-induced memory-impaired mice. We observed that EOFS (100, 200, and 400 mg/kg) ameliorated scopolamine-induced cognitive dysfunction. We also explored its mechanism of action by conducting an acetylcholinesterase (AChE) activity assay using the mouse whole brain and Western blot using the mouse hippocampal tissue. Western blot analysis and the ex vivo study revealed that EOFS increased the levels of phosphorylated extracellular signal-regulated kinase and cAMP response element-binding protein (CREB) and the levels of brain-derived neurotrophic factor (BDNF) expression in the hippocampus. It also inhibited AChE activity in the brain. Our findings suggest that EOFS would be useful for the treatment of cholinergic blockade-induced cognitive dysfunction.
ESTHER : Kwon_2018_J.Med.Food_21_971
PubMedSearch : Kwon_2018_J.Med.Food_21_971
PubMedID: 30044674

Title : Rannasangpei Is a Therapeutic Agent in the Treatment of Vascular Dementia - Wu_2016_Evid.Based.Complement.Alternat.Med_2016_2530105
Author(s) : Wu P , Luo Y , Zhen L , Hu X , Shang Y , Liao Y , Xue H , Huang F , Xiao W
Ref : Evid Based Complement Alternat Med , 2016 :2530105 , 2016
Abstract : Rannasangpei (RSNP) is used as a therapeutic agent in the treatment of cardiovascular diseases, neurological disorders, and neurodegeneration in China; however, its potential use in the treatment of vascular dementia (VD) was unclear. In this study, our aim was to examine the neuroprotective effect of RSNP in a VD rat model, which was induced by permanent bilateral common carotid artery occlusion (2VO). Four-week administration with two doses of RSNP was investigated in our study. Severe cognitive deficit in the VD model, which was confirmed in Morris water maze (MWM) test, was significantly restored by the administration of RSNP. ELISA revealed that the treatments with both doses of RSNP could reinstate the cholinergic activity in the VD animals by elevating the production of choline acetyltransferase (ChAT) and reducing the acetylcholinesterase (AChE); the treatment of RSNP could also reboot the level of superoxide dismutase (SOD) and decrease malondialdehyde (MDA). Moreover, Western blot and quantitative PCR (Q-PCR) results indicated that the RSNP could suppress the apoptosis in the hippocampus of the VD animals by increasing the expression ratio of B-cell lymphoma-2 (Bcl-2) to Bcl-2-associated X protein (Bax). These results suggested that RSNP might be a therapeutic agent in the treatment of vascular dementia in the future.
ESTHER : Wu_2016_Evid.Based.Complement.Alternat.Med_2016_2530105
PubMedSearch : Wu_2016_Evid.Based.Complement.Alternat.Med_2016_2530105
PubMedID: 27293454

Title : Polycyclic Polyprenylated Acylphloroglucinol Congeners Possessing Diverse Structures from Hypericum henryi - Yang_2015_J.Nat.Prod_78_885
Author(s) : Yang XW , Li MM , Liu X , Ferreira D , Ding Y , Zhang JJ , Liao Y , Qin HB , Xu G
Ref : Journal of Natural Products , 78 :885 , 2015
Abstract : Polycyclic polyprenylated acylphloroglucinols (PPAPs) are a class of hybrid natural products sharing the mevalonate/methylerythritol phosphate and polyketide biosynthetic pathways and showing considerable structural and bioactive diversity. In a systematic phytochemical investigation of Hypericum henryi, 40 PPAP-type derivatives, including the new compounds hyphenrones G-Q, were obtained. These compounds represent 12 different structural types, including four unusual skeletons exemplified by 5, 8, 10, and 17. The 12 different core structures found are explicable in terms of their biosynthetic origin. The structure of a known PPAP, perforatumone, was revised to hyphenrone A (5) by NMR spectroscopic and biomimetic synthesis methods. Several compounds exhibited inhibitory activities against acetylcholinesterase and human tumor cell lines. This study deals with the structural diversity, function, and biogenesis of natural PPAPs.
ESTHER : Yang_2015_J.Nat.Prod_78_885
PubMedSearch : Yang_2015_J.Nat.Prod_78_885
PubMedID: 25871261

Title : 1,9-seco-Bicyclic Polyprenylated Acylphloroglucinols from Hypericum uralum - Zhang_2015_J.Nat.Prod_78_3075
Author(s) : Zhang JJ , Yang XW , Liu X , Ma JZ , Liao Y , Xu G
Ref : Journal of Natural Products , 78 :3075 , 2015
Abstract : Hyperuralones C-H (1-6), six new 1,9-seco-bicyclic polyprenylated acylphloroglucinols (1,9-seco-BPAPs) derived from the normal polyprenylated acylphloroglucinols with a bicyclo[3.3.1]nonane-2,4,9-trione core, together with six known analogues, were isolated from the aerial parts of Hypericum uralum. The structures of 1-6 were elucidated on the basis of the interpretation of NMR and MS spectroscopic data. The structure of attenuatumione B, a known compound isolated from H. attenuatum, was revised to that of a 1,9-seco-BPAP by NMR spectroscopic analysis and previous biomimetic synthesis methods. The inhibitory activities of these isolates on acetylcholinesterase were tested, and compounds 1 and 2 exhibited moderate activities with IC50 values of 9.6 and 7.1 muM, respectively.
ESTHER : Zhang_2015_J.Nat.Prod_78_3075
PubMedSearch : Zhang_2015_J.Nat.Prod_78_3075
PubMedID: 26583263

Title : Hyphomonas beringensis sp. nov. and Hyphomonas chukchiensis sp. nov., isolated from surface seawater of the Bering Sea and Chukchi Sea - Li_2014_Antonie.Van.Leeuwenhoek_106_657
Author(s) : Li C , Lai Q , Li G , Dong C , Wang J , Liao Y , Shao Z
Ref : Antonie Van Leeuwenhoek , 106 :657 , 2014
Abstract : Two Gram-negative, non-spore-forming, oval to pear shaped motile strains, designated 25B14_1(T) and BH-BN04-4(T), isolated from surface seawater from the Bering Sea and Chukchi Sea, respectively, were subjected to polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strains 25B14_1(T) and BH-BN04-4(T) clustered together with Hyphomonas atlanticus 22II1-22F38(T) and Hyphomonas oceanitis DSM 5155(T), respectively, within genus Hyphomonas. Based on whole genome sequence analysis, the calculated DDH and ANIm values between strain 25B14_1(T) and BH-BN04-4(T) are 18.8 and 83.19% respectively. The calculated DDH values of strain 25B14_1(T) and BH-BN04-4(T) with seven type strains ranged from 18.2 to 19.9% and from 18.4 to 40.4%, respectively. The ANIm values of strain 25B14_1(T) and BH-BN04-4(T) with seven type strains ranged from 83.00 to 84.67% and from 83.14 to 90.58%, respectively. Both isolates were found to contain Q-11 as the predominant respiratory quinone. The major fatty acids of strain 25B14_1(T) were identified as C(16:0), C(17:0), C(18:1)omega7c-methyl and Summed Feature 8 (C(18:1)omega6c/omega7c as defined by MIDI), while in the case of strain BH-BN04-4(T) they were identified as C(16:0), C(18:1)omega7c-methyl and Summed Feature 8 (C(18:1)omega6c/omega7c). The G+C contents of 25B14_1(T) and BH-BN04-4(T) were determined to be 58.4 and 61.0 mol%, respectively. The combined phenotypic and genotypic data show that the two isolates each represent novel species of the genus Hyphomonas, for which the names Hyphomonas beringensis sp. nov. and Hyphomonas chukchiensis sp. nov. are proposed, with the type strain 25B14_1(T) (=MCCC 1A07321(T) = LMG 27914(T)) and BH-BN04-4(T) (=MCCC 1A07481(T) = LMG 27915(T)), respectively.
ESTHER : Li_2014_Antonie.Van.Leeuwenhoek_106_657
PubMedSearch : Li_2014_Antonie.Van.Leeuwenhoek_106_657
PubMedID: 25070064
Gene_locus related to this paper: 9rhob-a0a059fvx5 , 9rhob-a0a062ve25 , 9rhob-a0a059e3y9 , 9rhob-a0a062tww8 , 9rhob-a0a062u829 , 9rhob-a0a059e1b7 , 9rhob-a0a059g4l9 , 9rhob-a0a059f7b2 , 9rhob-a0a069e8d0 , 9rhob-a0a059g313 , 9rhob-a0a059g3u2 , 9rhob-a0a059fuw7 , 9rhob-a0a062ui03 , 9rhob-a0a059fgt4 , 9rhob-a0a059dz53 , 9rhob-a0a069e7i5 , 9rhob-a0a059fgq3 , 9rhob-a0a069e822 , 9rhob-a0a062vhu5 , 9rhob-a0a062uj80 , 9rhob-a0a062udx1 , 9rhob-a0a059eck3 , 9rhob-a0a062u513 , 9rhob-a0a062vh83 , 9rhob-a0a062u677 , 9rhob-a0a069e9p2 , 9rhob-a0a062ui16 , 9rhob-a0a059g4x1 , 9rhob-a0a062u908 , 9rhob-a0a062uqj8 , 9rhob-a0a062u917 , 9rhob-a0a059fty9 , 9rhob-a0a062uq79 , 9rhob-a0a059g214 , 9rhob-a0a062uez6 , 9rhob-a0a062v9r9 , 9rhob-a0a059g243 , 9rhob-a0a059fbn4 , 9rhob-a0a069e4q5 , 9rhob-a0a069e8h3 , 9rhob-a0a059dyu6 , 9rhob-a0a059fva5 , 9rhob-a0a059g4g8 , 9rhob-a0a059f9m8 , 9rhob-a0a062vgs9

Title : Memory-Enhancing Effects of the Crude Extract of Polygala tenuifolia on Aged Mice - Li_2014_Evid.Based.Complement.Alternat.Med_2014_392324
Author(s) : Li Z , Liu Y , Wang L , Liu X , Chang Q , Guo Z , Liao Y , Pan R , Fan TP
Ref : Evid Based Complement Alternat Med , 2014 :392324 , 2014
Abstract : Learning and memory disorders arise from distinct age-associated processes, and aging animals are often used as a model of memory impairment. The root of Polygala tenuifolia has been commonly used in some Asian countries as memory enhancer and its memory improvement has been reported in various animal models. However, there is less research to verify its effect on memory functions in aged animals. Herein, the memory-enhancing effects of the crude extract of Polygala tenuifolia (EPT) on normal aged mice were assessed by Morris water maze (MWM) and step-down passive avoidance tests. In MWM tests, the impaired spatial memory of the aged mice was partly reversed by EPT (100 and 200 mg/kg; P < 0.05) as compared with the aged control mice. In step-down tests, the nonspatial memory of the aged mice was improved by EPT (100 and 200 mg/kg; P < 0.05). Additionally, EPT could increase superoxide dismutase (SOD) and catalase (CAT) activities, inhibit monoamine oxidase (MAO) and acetyl cholinesterase (AChE) activities, and decrease the levels of malondialdehyde (MDA) in the brain tissue of the aged mice. The results showed that EPT improved memory functions of the aged mice probably via its antioxidant properties and via decreasing the activities of MAO and AChE.
ESTHER : Li_2014_Evid.Based.Complement.Alternat.Med_2014_392324
PubMedSearch : Li_2014_Evid.Based.Complement.Alternat.Med_2014_392324
PubMedID: 24744810

Title : Whole-genome sequencing of Oryza brachyantha reveals mechanisms underlying Oryza genome evolution - Chen_2013_Nat.Commun_4_1595
Author(s) : Chen J , Huang Q , Gao D , Wang J , Lang Y , Liu T , Li B , Bai Z , Luis Goicoechea J , Liang C , Chen C , Zhang W , Sun S , Liao Y , Zhang X , Yang L , Song C , Wang M , Shi J , Liu G , Liu J , Zhou H , Zhou W , Yu Q , An N , Chen Y , Cai Q , Wang B , Liu B , Min J , Huang Y , Wu H , Li Z , Zhang Y , Yin Y , Song W , Jiang J , Jackson SA , Wing RA , Chen M
Ref : Nat Commun , 4 :1595 , 2013
Abstract : The wild species of the genus Oryza contain a largely untapped reservoir of agronomically important genes for rice improvement. Here we report the 261-Mb de novo assembled genome sequence of Oryza brachyantha. Low activity of long-terminal repeat retrotransposons and massive internal deletions of ancient long-terminal repeat elements lead to the compact genome of Oryza brachyantha. We model 32,038 protein-coding genes in the Oryza brachyantha genome, of which only 70% are located in collinear positions in comparison with the rice genome. Analysing breakpoints of non-collinear genes suggests that double-strand break repair through non-homologous end joining has an important role in gene movement and erosion of collinearity in the Oryza genomes. Transition of euchromatin to heterochromatin in the rice genome is accompanied by segmental and tandem duplications, further expanded by transposable element insertions. The high-quality reference genome sequence of Oryza brachyantha provides an important resource for functional and evolutionary studies in the genus Oryza.
ESTHER : Chen_2013_Nat.Commun_4_1595
PubMedSearch : Chen_2013_Nat.Commun_4_1595
PubMedID: 23481403
Gene_locus related to this paper: orysa-Q6ZDG3 , orysa-q6h415 , orysj-q6yse8 , orysa-q33aq0 , orybr-j3l7k2 , orybr-j3m138 , orybr-j3l6m8 , orybr-j3m3b3 , orybr-j3l8d1 , orybr-j3kza5 , orybr-j3mnb5 , orybr-j3n4p4 , orybr-j3lg73 , orybr-j3l342 , orybr-j3msi2 , orybr-j3nb83 , orybr-j3mpc5

Title : Genome of Helicobacter pylori strain XZ274, an isolate from a tibetan patient with gastric cancer in China - Guo_2012_J.Bacteriol_194_4146
Author(s) : Guo Y , Wang H , Li Y , Song Y , Chen C , Liao Y , Ren L , Guo C , Tong W , Shen W , Chen M , Mao X , Guo G , Zou Q
Ref : Journal of Bacteriology , 194 :4146 , 2012
Abstract : The infection rate of Helicobacter pylori is high all over the world, especially in the Chinese Tibetan Plateau. Here, we report the genome sequence of Helicobacter pylori strain XZ274 isolated from a Tibetan patient with gastric cancer. The strain contains 1,634,138 bp with 1,654 coding sequences and a pXZ274 plasmid of 22,406 bp with 26 coding sequences. This is the first complete genome sequence of Helicobacter pylori from the Tibetan Plateau in China.
ESTHER : Guo_2012_J.Bacteriol_194_4146
PubMedSearch : Guo_2012_J.Bacteriol_194_4146
PubMedID: 22815458
Gene_locus related to this paper: helpy-o25061

Title : Complete genome sequence of Bacillus amyloliquefaciens XH7, which exhibits production of purine nucleosides - Yang_2011_J.Bacteriol_193_5593
Author(s) : Yang H , Liao Y , Wang B , Lin Y , Pan L
Ref : Journal of Bacteriology , 193 :5593 , 2011
Abstract : Here, we report the complete annotated genome sequence of Bacillus amyloliquefaciens XH7, which is used to produce purine nucleosides in industry. The genome sequence will allow for the characterization of the molecular mechanisms underlying its beneficial properties.
ESTHER : Yang_2011_J.Bacteriol_193_5593
PubMedSearch : Yang_2011_J.Bacteriol_193_5593
PubMedID: 21914895
Gene_locus related to this paper: baca2-a7z811 , bacas-e1ur92

Title : Genome sequence of Escherichia coli XH140A, which produces L-threonine - Yang_2011_J.Bacteriol_193_6090
Author(s) : Yang H , Liao Y , Wang B , Lin Y , Pan L
Ref : Journal of Bacteriology , 193 :6090 , 2011
Abstract : Here we report the draft annotated genome sequence of Escherichia coli XH140A, which is used to produce l-threonine in industry. The genome sequence will allow the characterization of the molecular mechanisms underlying its beneficial properties.
ESTHER : Yang_2011_J.Bacteriol_193_6090
PubMedSearch : Yang_2011_J.Bacteriol_193_6090
PubMedID: 21994923
Gene_locus related to this paper: ecoli-ybff , ecoli-ycfp , ecoli-yqia , ecoli-YfhR

Title : The genome of the mesopolyploid crop species Brassica rapa - Wang_2011_Nat.Genet_43_1035
Author(s) : Wang X , Wang H , Wang J , Sun R , Wu J , Liu S , Bai Y , Mun JH , Bancroft I , Cheng F , Huang S , Li X , Hua W , Freeling M , Pires JC , Paterson AH , Chalhoub B , Wang B , Hayward A , Sharpe AG , Park BS , Weisshaar B , Liu B , Li B , Tong C , Song C , Duran C , Peng C , Geng C , Koh C , Lin C , Edwards D , Mu D , Shen D , Soumpourou E , Li F , Fraser F , Conant G , Lassalle G , King GJ , Bonnema G , Tang H , Belcram H , Zhou H , Hirakawa H , Abe H , Guo H , Jin H , Parkin IA , Batley J , Kim JS , Just J , Li J , Xu J , Deng J , Kim JA , Yu J , Meng J , Min J , Poulain J , Hatakeyama K , Wu K , Wang L , Fang L , Trick M , Links MG , Zhao M , Jin M , Ramchiary N , Drou N , Berkman PJ , Cai Q , Huang Q , Li R , Tabata S , Cheng S , Zhang S , Sato S , Sun S , Kwon SJ , Choi SR , Lee TH , Fan W , Zhao X , Tan X , Xu X , Wang Y , Qiu Y , Yin Y , Li Y , Du Y , Liao Y , Lim Y , Narusaka Y , Wang Z , Li Z , Xiong Z , Zhang Z
Ref : Nat Genet , 43 :1035 , 2011
Abstract : We report the annotation and analysis of the draft genome sequence of Brassica rapa accession Chiifu-401-42, a Chinese cabbage. We modeled 41,174 protein coding genes in the B. rapa genome, which has undergone genome triplication. We used Arabidopsis thaliana as an outgroup for investigating the consequences of genome triplication, such as structural and functional evolution. The extent of gene loss (fractionation) among triplicated genome segments varies, with one of the three copies consistently retaining a disproportionately large fraction of the genes expected to have been present in its ancestor. Variation in the number of members of gene families present in the genome may contribute to the remarkable morphological plasticity of Brassica species. The B. rapa genome sequence provides an important resource for studying the evolution of polyploid genomes and underpins the genetic improvement of Brassica oil and vegetable crops.
ESTHER : Wang_2011_Nat.Genet_43_1035
PubMedSearch : Wang_2011_Nat.Genet_43_1035
PubMedID: 21873998
Gene_locus related to this paper: braol-Q8GTM3 , braol-Q8GTM4 , brarp-m4ei94 , brarp-m4c988 , brana-a0a078j4a9 , brana-a0a078e1m0 , brana-a0a078cd75 , brarp-m4dwa6 , brana-a0a078j4f0 , brana-a0a078cus4 , brana-a0a078f8c2 , brana-a0a078jql1 , brana-a0a078dgj3 , brana-a0a078hw50 , brana-a0a078cuu0 , brana-a0a078dfa9 , brana-a0a078ic91 , brarp-m4ctw3 , brana-a0a078ca65 , brana-a0a078ctc8 , brana-a0a078h021 , brana-a0a078jx23 , brarp-m4da84 , brarp-m4dwr7 , brana-a0a078dh94 , brana-a0a078h612 , brana-a0a078j2t3 , braol-a0a0d3dpb2 , braol-a0a0d3dx76 , brana-a0a078jxa8 , brana-a0a078i2k3 , brarp-m4cwq4 , brarp-m4dcj8 , brarp-m4eh17 , brarp-m4eey4 , brarp-m4dnj8 , brarp-m4ey83 , brarp-m4ey84

Title : Repression of AtCLH1 expression results in a decrease in the ratio of chlorophyll a\/b but doesnot affect the rate of chlorophyll degradation during leaf senescence - Zhou_2007_Zhi.Wu.Sheng.Li.Yu.Fen.Zi.Sheng.Wu.Xue.Xue.Bao_33_596
Author(s) : Zhou X , Liao Y , Ren GD , Zhang YY , Chen WJ , Kuai BK
Ref : Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao , 33 :596 , 2007
Abstract : To explore the possible regulatory role of chlorophyllase (Chlase) in chlorophyll (Chl) degradation during leaf senescence, RNAi Arabidopsis (Arabidopsis thaliana) plants were constructed to repress the expressions of AtCLH1 and/or AtCLH2. Transcript levels of AtCLH1 and/or AtCLH2 were dramatically lowered and Chlase activity was correspondingly inhibited, but the Chl degradation kinetics was not affected in the RNAi plants. Results of further analysis indicated that the Chl a/b ratio decreased in AtCLH1 RNAi lines, in comparison with the increasing Chl a/b ratio in the wide type during leaf senescence. In addition, an induced Chlase activity was consistently detected at the initial stage of senescence in all the plants examined. In contrast, transcript levels of both AtCLH1 and AtCLH2 decreased dramatically upon the initiation of senescence in both the wide-type and the RNAi plants. Interestingly, compared with the wide type, lower but still significant transcript levels of the RNAi targeted Chlase gene(s) were sustained during the whole period of dark incubation in all the three RNAi lines examined, indicating the functioning of some compensatively regulating mechanism. Based on these results, along with related reports, we conclude that Chlase might be required at the initial stage of leaf senescence, quite likely playing a role in converting Chl b to a.
ESTHER : Zhou_2007_Zhi.Wu.Sheng.Li.Yu.Fen.Zi.Sheng.Wu.Xue.Xue.Bao_33_596
PubMedSearch : Zhou_2007_Zhi.Wu.Sheng.Li.Yu.Fen.Zi.Sheng.Wu.Xue.Xue.Bao_33_596
PubMedID: 18349515

Title : New (sulfonyloxy)piperazinyldibenzazepines as potential atypical antipsychotics: chemistry and pharmacological evaluation - Liao_1999_J.Med.Chem_42_2235
Author(s) : Liao Y , Venhuis BJ , Rodenhuis N , Timmerman W , Wikstrom H , Meier E , Bartoszyk GD , Bottcher H , Seyfried CA , Sundell S
Ref : Journal of Medicinal Chemistry , 42 :2235 , 1999
Abstract : A series of 2- or 8-trifluoromethylsulfonyloxy (TfO) and 2- or 8-methylsulfonyloxy (MsO) 11-piperazinyldibenzodiazepines, -oxazepines, and -thiazepines were synthesized and evaluated in pharmacological models for their potential clozapine-like properties. In receptor binding assays, the 2-TfO analogues (18a, GMC2-83; 24, GMC3-06; and previously reported GMC1-169, 9a) of the dibenzazepines have profiles comparable to that of clozapine, acting on a variety of CNS receptors except they lack M1 receptor affinity. Introduction of 2-TfO to clozapine leads to compound 9e (GMC61-39) which has a similar binding profile as that of clozapine including having M1 receptor affinity. Interestingly, the MsO analogues, as well as the 8-TfO analogues, have no or weak dopaminergic and serotonergic affinities, but all 8-sulfonyloxy analogues do have M1 affinities. In behavioral studies performed to indicate the potential antipsychotic efficacy and the propensity to induce EPS, 2-TfO analogues blocked effectively the apomorphine-induced climbing in mice in a dose-dependent manner with ED50 values (mg/kg) of 2.1 sc for 9a, 1.3 po for 18a, 2.6 sc for 24, and 8.2 sc for 9e. On the other hand, they showed a clear dose separation with regard to their ED50 values (mg/kg) for indicating catalepsy in rats (>44 sc for 9a, 28 po for 18a, 30 sc for 24, and >50 sc for 9e, respectively), thus implicating a more favorable therapeutic ratio (K/A, ED50 climbing/ED50 catalepsy) in comparison with typical neuroleptics such as haloperidol and isoclozapine. Furthermore, compound 18a was also demonstrated to be an orally potent DA antagonist with an ED50 value of 0.7 mg/kg po in the ex vivo L-DOPA accumulation model. The present study contributes to the SAR of 11-piperazinyldibenzazepines, and the 2-TfO analogues of 11-piperazinyldibenzazepines are promising candidates as clozapine-like atypical antipsychotics with low propensity to induce EPS.
ESTHER : Liao_1999_J.Med.Chem_42_2235
PubMedSearch : Liao_1999_J.Med.Chem_42_2235
PubMedID: 10377229

Title : Synthesis and pharmacological evaluation of triflate-substituted analogues of clozapine: identification of a novel atypical neuroleptic - Liao_1997_J.Med.Chem_40_4146
Author(s) : Liao Y , DeBoer P , Meier E , Wikstrom H
Ref : Journal of Medicinal Chemistry , 40 :4146 , 1997
Abstract : The trifluoromethanesulfonyloxy (TfO) analogues 3 and 4 of 8-chloro-11-(4-methyl-1-piperazinyl)-5H-dibenzo[b,e][1,4]diazepine (clozapine, 1) and its 2-chloro isomer (iso-clozapine, 2), respectively, were synthesized via their OMe and OH analogues with the conventional synthetic method of the tricyclic dibenzodiazepines and evaluated pharmacologically along with their parent drugs. The binding profile of the 2-OTf analogue (4) is comparable to the binding profile of 1, although the affinity for the dopamine (DA) D2 receptors is higher (IC50 values are 31 nM and 330 nM for compounds 4 and 1, respectively). Interestingly, no notable affinity for muscarinic receptors could be detected in compound 4. On the contrary, the 8-OTf analogue 3 only displayed affinity for muscarinic M1 receptors (IC50 value 35 nM) and no affinity (IC50 value > 500 nM) for the other receptors tested. The 10 mumol/kg sc dose, but not the 10 mumol/kg po dose, of compound 4 stimulated the output of DA. Increases of 80% and 35% in DOPAC output from the dorsal striatum were seen after sc and po administrations of 10 mumol/kg of compound 4, respectively. Doses up to 100 mumol/kg of compound 3 had no effect on either parameter. Doses up to 100 mumol/kg of compound 4 were not cataleptogenic, but significantly decreased apomorphine-induced locomotor activity. In conclusion, compound 4 (GMC1-169) is a new clozapine-like neuroleptic candidate, which is lacking anticholinergic properties and displays a higher potency, as compared to clozapine (1) itself.
ESTHER : Liao_1997_J.Med.Chem_40_4146
PubMedSearch : Liao_1997_J.Med.Chem_40_4146
PubMedID: 9406603