PolyAspartate-hydrolase
Relationship
Comment
Poly(aspartate) (PAA) is a bio-based, biocompatible, biodegradable alternative to polycarboxylate of poly(acrylate). Bacterial PAA-hydrolyzing enzyme can also perform synthesis of poly(alpha-ethyl beta-aspartate). This family is close to PHB depolymerase. The LpqC, poly(3-hydroxybutyrate) depolymerase from Bordetella parapertussis (CASP Target) (structure 3D0K) belongs to this family. Polyaspartic acid (PASA) is also a natural polymer as fragment of larger proteins with length up to 50 amino acids. Synthetic tPAA(thermally synthesized PAA) consists of beta-amide (70%) and alpha-amide,(30%) D- and L-aspartate units; the alpha- and beta-amide units are randomly distributed. PAA hydrolase-1 is an alpha beta hydrolase inhibited by PMSF and DFP. PAA hydrolase-2 is closer to metallo carboxypeptidase inhibited by DFP and PMSF
Database
Interpro : No interpro
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF12695 Abhydrolase_5
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 3D0K | Crystal structure of the LpqC, poly(3-hydroxybutyrate) depolymerase from Bordetella parapertussis (CASP Target) | borpa-Q7W3B7 |
| 6VE6 | A structural characterization of poly(aspartic acid) hydrolase-1 PahZ1 from Sphingomonas sp. KT-1. | 9sphn-q7wsc1 |
| 8DNB | WITHDRAWN Expanding structural knowledge of poly(aspartic acid) hydrolases: Structure determination of Pedobacter sp. KP-2 PahZ1 | 9sphi-b6vqa9 |
| Gene_locus | Name | Species |
|---|---|---|
| 9acto-d9ukw7 | Streptomyces sp. SPB78 Poly(Aspartic acid) hydrolase | Streptomyces sp. SPB78 |
| 9acto-d9wjn1 | Streptomyces himastatinicus ATCC 53653 Putative uncharacterized protein | Streptomyces himastatinicus ATCC 53653 |
| 9alte-a6ez29 | Marinobacter algicola DG893 Putative uncharacterized protein | Marinobacter algicola DG893 |
| 9alte-k6y2r8 | Glaciecola pallidula DSM 14239 = ACAM 615. Uncharacterized protein | Glaciecola pallidula DSM 14239 = ACAM 615 |
| 9bace-b3c5f6 | Bacteroides intestinalis DSM 17393 Bacteroides cellulosilyticus DSM 14838 Putative uncharacterized protein | Bacteroides intestinalis DSM 17393 |
| 9bact-a0a1v6dh18 | Verrucomicrobia bacterium ADurb.Bin118. Alpha\/beta hydrolase family protein | Verrucomicrobia bacterium ADurb.Bin118 |
| 9bact-a3i1k2 | Algoriphagus sp. PR1 Putative uncharacterized protein | Algoriphagus sp. PR1 |
| 9bact-d1pue9 | Prevotella bergensis DSM 17361 Putative uncharacterized protein | Prevotella bergensis DSM 17361 |
| 9brad-d6v1p2 | Afipia sp. 1NLS2 Putative poly(Aspartic acid) hydrolase | Afipia sp. 1NLS2 |
| 9caul-f4qiu2 | Asticcacaulis biprosthecum C19 Poly3-Hydroxybutyrate Depolymerase | Asticcacaulis biprosthecum C19 |
| 9clot-c0cw58 | Clostridium asparagiforme DSM 15981 Putative uncharacterized protein | Clostridium asparagiforme DSM 15981 |
| 9cyan-a3inp3 | Cyanothece sp. CCY0110. Uncharacterized protein | Cyanothece sp. CCY0110 |
| 9delt-a0a0d2g825 | Dethiosulfatarculus sandiegensis. Uncharacterized protein | Dethiosulfatarculus sandiegensis |
| 9flao-a0a1k2irq9 | Flaviramulus basaltis. Chlorophyllase | Flaviramulus basaltis |
| 9flao-a3j5l9 | Flavobacteria bacterium BAL38 Putative uncharacterized protein | Flavobacteria bacterium BAL38 |
| 9flao-a4bw79 | Polaribacter irgensii 23-P Putative uncharacterized protein | Polaribacter irgensii 23-P |
| 9flao-a8umh7 | Flavobacteriales bacterium ALC-1 Putative uncharacterized protein | Flavobacteriales bacterium ALC-1 |
| 9flao-q1vqh8 | Psychroflexus torquis (strain ATCC 700755 \/ ACAM 623) Putative uncharacterized protein | Psychroflexus torquis ATCC 700755 |
| 9gamm-a0a0s2kdi6 | Pseudohongiella spirulinae. Uncharacterized protein | Pseudohongiella spirulinae |
| 9gamm-a0a193lfy4 | Woeseia oceani. Uncharacterized protein | Woeseia oceani |
| 9mico-a0a0q9sj09 | Phycicoccus sp. Uncharacterized protein | Phycicoccus sp. Soil803 |
| 9prot-a0a1f3axs2 | Alphaproteobacteria bacterium RIFOXYD12_FULL_60_8. Uncharacterized protein | Alphaproteobacteria bacterium RIFOXYD12_FULL_60_8 |
| 9prot-a0a1f4a0e8 | Betaproteobacteria bacterium RIFCSPLOWO2_02_64_14. Uncharacterized protein | Betaproteobacteria bacterium RIFCSPLOWO2_02_64_14 |
| 9prot-d5rji3 | Roseomonas cervicalis ATCC 49957 Poly(Aspartic acid) hydrolase | Roseomonas cervicalis ATCC 49957 |
| 9psed-f2zpn2 | Pseudomonas syringae pv. oryzae str. 1_6 Putative uncharacterized protein | Pseudomonas syringae pv. oryzae str. 1_6 |
| 9rhob-a0a059fbn4 | Hyphomonas johnsonii MHS-2. Uncharacterized protein | Hyphomonas johnsonii MHS-2 |
| 9rhob-a0a069e4q5 | Hyphomonas adhaerens MHS-3. Uncharacterized protein | Hyphomonas adhaerens MHS-3 |
| 9rhob-c9ctk5 | Silicibacter sp. TrichCH4B Putative uncharacterized protein | Silicibacter sp. TrichCH4B |
| 9sphi-b6vqa9 |  Pedobacter sp. KP-2 Poly(Aspartic acid) hydrolase-1 |
Pedobacter sp. KP-2 |
| 9sphn-a0a074m3d8 | Erythrobacter sp. JL475. Uncharacterized protein | Erythrobacter sp. JL475 |
| 9sphn-a0a1d7nm80 | Porphyrobacter sp. LM 6. Uncharacterized protein | Porphyrobacter sp. LM 6 |
| 9sphn-a0a1g3k9z6 | Sphingopyxis sp. RIFCSPHIGHO2_01_FULL_65_24. Uncharacterized protein | Sphingopyxis sp. RIFCSPHIGHO2_01_FULL_65_24 |
| 9sphn-a0a1n6cm40 | Sphingorhabdus marina DSM 22363. Uncharacterized protein | Sphingorhabdus marina DSM 22363 |
| 9sphn-q7wsc1 | Sphingomonas sp. KT-1. Poly(Aspartic acid) hydrolase-1 |
Sphingomonas sp. KT-1 |
| acicj-a5g251 | Acidiphilium cryptum (strain JF-5) Putative uncharacterized protein | Acidiphilium cryptum |
| acima-f0j5m5 | Acidiphilium multivorum (strain DSM 11245 \/ JCM 8867 \/ AIU301) Putative uncharacterized protein | Acidiphilium multivorum |
| agrsh-f0lfg1 | Agrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3)) Putative uncharacterized protein | Agrobacterium sp. |
| amymu-d8i4g6 | Amycolatopsis mediterranei (strain U-32) Putative uncharacterized protein | Amycolatopsis mediterranei |
| bacov-d4wwr0 | Bacteroides ovatus SD CC 2a Bacteroides sp. (2_1_22\; D1\; D22\; D2) Bacteroides xylanisolvens (XB1A\; SD CC 1b) Putative lipoprotein | Bacteroides ovatus |
| borpa-Q7W3B7 | Bordetella parapertussis LpqC, poly(3-hydroxybutyrate) depolymerase (CASP Target) |
Bordetella parapertussis |
| braja-q89y23 | Bradyrhizobium japonicum Bll0132 protein | Bradyrhizobium japonicum |
| burgb-c5ail4 | Burkholderia glumae (strain BGR1) Putative uncharacterized protein | Burkholderia glumae |
| cupnh-q0k4q3 | Cupriavidus necator (strain ATCC 17699 \/ H16 \/ DSM 428 \/ Stanier 337)(Ralstonia eutropha) Conserved hypothetical membrane associated protein | Cupriavidus necator |
| cuptr-b2ai31 | Cupriavidus taiwanensis (strain R1 \/ LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) Putative uncharacterized protein | Cupriavidus taiwanensis |
| gemat-c1abv8 | Gemmatimonas aurantiaca (strain T-27 \/ DSM 14586 \/ JCM 11422 \/ NBR 100505) Putative uncharacterized protein | Gemmatimonas aurantiaca |
| gluox-q5fn27 | Gluconobacter oxydans (Gluconobacter suboxydans) Putative uncharacterized protein | Gluconobacter oxydans |
| marah-e4plh6 | Marinobacter adhaerens (strain HP15) Putative uncharacterized protein | Marinobacter adhaerens |
| marsh-a4av43 | Maribacter sp. (strain HTCC2170 \/ KCCM 42371) Putative uncharacterized protein | Maribacter sp. |
| metb6-a7i919 | Methanoregula boonei (strain DSM 21154 \/ JCM 14090 \/ 6A8). Uncharacterized protein | Methanoregula boonei |
| penmq-b6qm40 | Penicillium marneffei (strain ATCC 18224 \/ CBS 334.59 \/ QM 7333) Putative uncharacterized protein | Penicillium marneffei |
| psepf-q3k6f3 | Pseudomonas fluorescens (strain Pf0-1) Putative exported protein | Pseudomonas fluorescens |
| ralso-b5slf2 | Ralstonia solanacearum (IPO1609\; UW551) Putative uncharacterized protein | Ralstonia solanacearum |
| ralso-d8njk6 | Ralstonia solanacearum (Pseudomonas solanacearum) Putative uncharacterized protein | Ralstonia solanacearum |
| robbh-a4ck49 | Robiginitalea biformata (strain ATCC BAA-864 \/ HTCC2501 \/ KCTC 12146) Putative uncharacterized protein | Robiginitalea biformata |
| shewm-b1klb6 | Shewanella woodyi (strain ATCC 51908 \/ MS32) Putative uncharacterized protein | Shewanella woodyi |
| strbb-d7cds1 | Streptomyces bingchenggensis (strain BCW-1) Poly aspartic acid hydrolase | Streptomyces bingchenggensis |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| PHB | poly(3-hydroxybutyrate) | psesp-PHBDP1 aspfu-q4w9v8 crysp-Q874E9 alceu-phbc |
| Polyaspartic-acid | poly-L-aspartic acid |
| Inhibitor | Chemical Nomenclature | Proteins |
|---|---|---|
| DFP | Diisopropylphosphorofluoridate | arcfu-AF1763 human-DPP4 human-ACHE human-BCHE human-PLA2G7 bacce-BC3734 ecoli-IROE fusso-cutas human-FAP human-CTSA aspni-FAEA |
| PMSF | Phenylmethylsulfonyl fluoride | theet-q3ch51 emeni-q5avy9 human-ABHD10 human-PREPL myctu-Rv1430 psefl-cxest 9bact-E40 thefu-q6a0i4 ecoli-Aes orysj-Q10QA5 bac25-mglp 9bact-Q0GMU2 bacsu-FENB psepu-clcd1 thema-TM0077 bacsu-RsbQ ecoli-bioh drome-CG9542 human-FAP tubmm-TmEst2 human-NOTUM cutac-e6dae5 9gamm-urc15994 9baci-EstD9 geost-est30 pholl-AntI |
References (3)
| Title : Poly(aspartate) hydrolases: biochemical properties and applications - Hiraishi_2011_Appl.Microbiol.Biotechnol_91_895 |
| Author(s) : Hiraishi T , Maeda M |
| Ref : Applied Microbiology & Biotechnology , 91 :895 , 2011 |
| Abstract : |
| PubMedSearch : Hiraishi_2011_Appl.Microbiol.Biotechnol_91_895 |
| PubMedID: 21713512 |
| Title : Cloning of poly(aspartic acid) (PAA) hydrolase-1 gene from Pedobacter sp. KP-2 and hydrolysis of thermally synthesized PAA by its gene product - Hiraishi_2009_Macromol.Biosci_9_10 |
| Author(s) : Hiraishi T , Masuda E , Kanayama N , Nagata M , Doi Y , Abe H , Maeda M |
| Ref : Macromol Biosci , 9 :10 , 2009 |
| Abstract : |
| PubMedSearch : Hiraishi_2009_Macromol.Biosci_9_10 |
| PubMedID: 18756460 |
| Gene_locus related to this paper: 9sphi-b6vqa9 |
| Title : Genetic Analysis and Characterization of Poly(aspartic acid) Hydrolase-1 from Sphingomonas sp. KT-1 - Hiraishi_2003_Biomacromolecules_4_80 |
| Author(s) : Hiraishi T , Kajiyama M , Tabata K , Yamato I , Doi Y |
| Ref : Biomacromolecules , 4 :80 , 2003 |
| Abstract : |
| PubMedSearch : Hiraishi_2003_Biomacromolecules_4_80 |
| PubMedID: 12523851 |
| Gene_locus related to this paper: 9sphn-q7wsc1 |