Family: PolyAspartate-hydrolase

Block: X

Parent Family: Abhydrolase_5


Poly(aspartate) (PAA) is a bio-based, biocompatible, biodegradable alternative to polycarboxylate of poly(acrylate). Bacterial PAA-hydrolyzing enzyme can also perform synthesis of poly(alpha-ethyl beta-aspartate). This family is close to PHB depolymerase. The LpqC, poly(3-hydroxybutyrate) depolymerase from Bordetella parapertussis (CASP Target) (structure 3D0K) belongs to this family. Polyaspartic acid (PASA) is also a natural polymer as fragment of larger proteins with length up to 50 amino acids. Synthetic tPAA(thermally synthesized PAA) consists of beta-amide (70%) and alpha-amide,(30%) D- and L-aspartate units; the alpha- and beta-amide units are randomly distributed. PAA hydrolase-1 is an alpha beta hydrolase inhibited by PMSF and DFP. PAA hydrolase-2 is closer to metallo carboxypeptidase inhibited by DFP and PMSF


Interpro : No interpro


Pdoc : No Pdoc

Pfam : PF12695 Abhydrolase_5

Prints : No Print

EC Number : No EC Number


Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
8DNB WITHDRAWN Expanding structural knowledge of poly(aspartic acid) hydrolases: Structure determination of Pedobacter sp. KP-2 PahZ1 9sphi-b6vqa9
6VE6 A structural characterization of poly(aspartic acid) hydrolase-1 PahZ1 from Sphingomonas sp. KT-1. 9sphn-q7wsc1
3D0K Crystal structure of the LpqC, poly(3-hydroxybutyrate) depolymerase from Bordetella parapertussis (CASP Target) borpa-Q7W3B7

Gene_locus Name Species
9acto-d9ukw7 Streptomyces sp. SPB78 Poly(Aspartic acid) hydrolase Streptomyces sp. SPB78
9acto-d9wjn1 Streptomyces himastatinicus ATCC 53653 Putative uncharacterized protein Streptomyces himastatinicus ATCC 53653
9alte-a6ez29 Marinobacter algicola DG893 Putative uncharacterized protein Marinobacter algicola DG893
9bace-b3c5f6 Bacteroides intestinalis DSM 17393 Bacteroides cellulosilyticus DSM 14838 Putative uncharacterized protein Bacteroides intestinalis DSM 17393
9bact-a3i1k2 Algoriphagus sp. PR1 Putative uncharacterized protein Algoriphagus sp. PR1
9bact-d1pue9 Prevotella bergensis DSM 17361 Putative uncharacterized protein Prevotella bergensis DSM 17361
9brad-d6v1p2 Afipia sp. 1NLS2 Putative poly(Aspartic acid) hydrolase Afipia sp. 1NLS2
9caul-f4qiu2 Asticcacaulis biprosthecum C19 Poly3-Hydroxybutyrate Depolymerase Asticcacaulis biprosthecum C19
9clot-c0cw58 Clostridium asparagiforme DSM 15981 Putative uncharacterized protein Clostridium asparagiforme DSM 15981
9flao-a3j5l9 Flavobacteria bacterium BAL38 Putative uncharacterized protein Flavobacteria bacterium BAL38
9flao-a4bw79 Polaribacter irgensii 23-P Putative uncharacterized protein Polaribacter irgensii 23-P
9flao-a8umh7 Flavobacteriales bacterium ALC-1 Putative uncharacterized protein Flavobacteriales bacterium ALC-1
9flao-q1vqh8 Psychroflexus torquis (strain ATCC 700755 \/ ACAM 623) Putative uncharacterized protein Psychroflexus torquis ATCC 700755
9prot-d5rji3 Roseomonas cervicalis ATCC 49957 Poly(Aspartic acid) hydrolase Roseomonas cervicalis ATCC 49957
9psed-f2zpn2 Pseudomonas syringae pv. oryzae str. 1_6 Putative uncharacterized protein Pseudomonas syringae pv. oryzae str. 1_6
9rhob-c9ctk5 Silicibacter sp. TrichCH4B Putative uncharacterized protein Silicibacter sp. TrichCH4B
9sphi-b6vqa9 Pedobacter sp. KP-2 Poly(Aspartic acid) hydrolase-1 Pedobacter sp. KP-2
acicj-a5g251 Acidiphilium cryptum (strain JF-5) Putative uncharacterized protein Acidiphilium cryptum
acima-f0j5m5 Acidiphilium multivorum (strain DSM 11245 \/ JCM 8867 \/ AIU301) Putative uncharacterized protein Acidiphilium multivorum
agrsh-f0lfg1 Agrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3)) Putative uncharacterized protein Agrobacterium sp.
amymu-d8i4g6 Amycolatopsis mediterranei (strain U-32) Putative uncharacterized protein Amycolatopsis mediterranei
bacov-d4wwr0 Bacteroides ovatus SD CC 2a Bacteroides sp. (2_1_22\; D1\; D22\; D2) Bacteroides xylanisolvens (XB1A\; SD CC 1b) Putative lipoprotein Bacteroides ovatus
borpa-Q7W3B7 Bordetella parapertussis LpqC, poly(3-hydroxybutyrate) depolymerase (CASP Target) Bordetella parapertussis
braja-q89y23 Bradyrhizobium japonicum Bll0132 protein Bradyrhizobium japonicum
burgb-c5ail4 Burkholderia glumae (strain BGR1) Putative uncharacterized protein Burkholderia glumae
cupnh-q0k4q3 Cupriavidus necator (strain ATCC 17699 \/ H16 \/ DSM 428 \/ Stanier 337)(Ralstonia eutropha) Conserved hypothetical membrane associated protein Cupriavidus necator
cuptr-b2ai31 Cupriavidus taiwanensis (strain R1 \/ LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) Putative uncharacterized protein Cupriavidus taiwanensis
gemat-c1abv8 Gemmatimonas aurantiaca (strain T-27 \/ DSM 14586 \/ JCM 11422 \/ NBR 100505) Putative uncharacterized protein Gemmatimonas aurantiaca
gluox-q5fn27 Gluconobacter oxydans (Gluconobacter suboxydans) Putative uncharacterized protein Gluconobacter oxydans
marah-e4plh6 Marinobacter adhaerens (strain HP15) Putative uncharacterized protein Marinobacter adhaerens
marsh-a4av43 Maribacter sp. (strain HTCC2170 \/ KCCM 42371) Putative uncharacterized protein Maribacter sp.
penmq-b6qm40 Penicillium marneffei (strain ATCC 18224 \/ CBS 334.59 \/ QM 7333) Putative uncharacterized protein Penicillium marneffei
psepf-q3k6f3 Pseudomonas fluorescens (strain Pf0-1) Putative exported protein Pseudomonas fluorescens
ralso-b5slf2 Ralstonia solanacearum (IPO1609\; UW551) Putative uncharacterized protein Ralstonia solanacearum
ralso-d8njk6 Ralstonia solanacearum (Pseudomonas solanacearum) Putative uncharacterized protein Ralstonia solanacearum
robbh-a4ck49 Robiginitalea biformata (strain ATCC BAA-864 \/ HTCC2501 \/ KCTC 12146) Putative uncharacterized protein Robiginitalea biformata
shewm-b1klb6 Shewanella woodyi (strain ATCC 51908 \/ MS32) Putative uncharacterized protein Shewanella woodyi
strbb-d7cds1 Streptomyces bingchenggensis (strain BCW-1) Poly aspartic acid hydrolase Streptomyces bingchenggensis
9flao-a0a1k2irq9 Flaviramulus basaltis. Chlorophyllase Flaviramulus basaltis
9sphn-q7wsc1 Sphingomonas sp. KT-1. Poly(Aspartic acid) hydrolase-1 Sphingomonas sp. KT-1
9sphn-a0a1g3k9z6 Sphingopyxis sp. RIFCSPHIGHO2_01_FULL_65_24. Uncharacterized protein Sphingopyxis sp. RIFCSPHIGHO2_01_FULL_65_24
9sphn-a0a1d7nm80 Porphyrobacter sp. LM 6. Uncharacterized protein Porphyrobacter sp. LM 6
9sphn-a0a074m3d8 Erythrobacter sp. JL475. Uncharacterized protein Erythrobacter sp. JL475
9gamm-a0a193lfy4 Woeseia oceani. Uncharacterized protein Woeseia oceani
9rhob-a0a059fbn4 Hyphomonas johnsonii MHS-2. Uncharacterized protein Hyphomonas johnsonii MHS-2
9gamm-a0a0s2kdi6 Pseudohongiella spirulinae. Uncharacterized protein Pseudohongiella spirulinae
9alte-k6y2r8 Glaciecola pallidula DSM 14239 = ACAM 615. Uncharacterized protein Glaciecola pallidula DSM 14239 = ACAM 615
9sphn-a0a1n6cm40 Sphingorhabdus marina DSM 22363. Uncharacterized protein Sphingorhabdus marina DSM 22363
9delt-a0a0d2g825 Dethiosulfatarculus sandiegensis. Uncharacterized protein Dethiosulfatarculus sandiegensis
9cyan-a3inp3 Cyanothece sp. CCY0110. Uncharacterized protein Cyanothece sp. CCY0110
9rhob-a0a069e4q5 Hyphomonas adhaerens MHS-3. Uncharacterized protein Hyphomonas adhaerens MHS-3
9bact-a0a1v6dh18 Verrucomicrobia bacterium ADurb.Bin118. Alpha\/beta hydrolase family protein Verrucomicrobia bacterium ADurb.Bin118
metb6-a7i919 Methanoregula boonei (strain DSM 21154 \/ JCM 14090 \/ 6A8). Uncharacterized protein Methanoregula boonei
9prot-a0a1f3axs2 Alphaproteobacteria bacterium RIFOXYD12_FULL_60_8. Uncharacterized protein Alphaproteobacteria bacterium RIFOXYD12_FULL_60_8
9prot-a0a1f4a0e8 Betaproteobacteria bacterium RIFCSPLOWO2_02_64_14. Uncharacterized protein Betaproteobacteria bacterium RIFCSPLOWO2_02_64_14
9mico-a0a0q9sj09 Phycicoccus sp. Uncharacterized protein Phycicoccus sp. Soil803

Substrate Chemical Nomenclature Proteins
Polyaspartic-acid poly-L-aspartic acid
PHB poly(3-hydroxybutyrate) psesp-PHBDP1    aspfu-q4w9v8    crysp-Q874E9    alceu-phbc

Inhibitor Chemical Nomenclature Proteins
DFP Diisopropylphosphorofluoridate arcfu-AF1763    human-DPP4    human-ACHE    human-BCHE    human-PLA2G7    bacce-BC3734    ecoli-IROE    fusso-cutas    human-FAP    human-CTSA    aspni-FAEA
PMSF Phenylmethylsulfonyl fluoride theet-q3ch51    emeni-q5avy9    human-ABHD10    human-PREPL    myctu-Rv1430    psefl-cxest    9bact-E40    thefu-q6a0i4    ecoli-Aes    orysj-Q10QA5    bac25-mglp    9bact-Q0GMU2    bacsu-FENB    psepu-clcd1    thema-TM0077    bacsu-RsbQ    ecoli-bioh    drome-CG9542    human-FAP    tubmm-TmEst2    human-NOTUM    cutac-e6dae5    9gamm-urc15994    9baci-EstD9    geost-est30    pholl-AntI

References (3)

Title : Poly(aspartate) hydrolases: biochemical properties and applications - Hiraishi_2011_Appl.Microbiol.Biotechnol_91_895
Author(s) : Hiraishi T , Maeda M
Ref : Applied Microbiology & Biotechnology , 91 :895 , 2011
Abstract :
PubMedSearch : Hiraishi_2011_Appl.Microbiol.Biotechnol_91_895
PubMedID: 21713512

Title : Cloning of poly(aspartic acid) (PAA) hydrolase-1 gene from Pedobacter sp. KP-2 and hydrolysis of thermally synthesized PAA by its gene product - Hiraishi_2009_Macromol.Biosci_9_10
Author(s) : Hiraishi T , Masuda E , Kanayama N , Nagata M , Doi Y , Abe H , Maeda M
Ref : Macromol Biosci , 9 :10 , 2009
Abstract :
PubMedSearch : Hiraishi_2009_Macromol.Biosci_9_10
PubMedID: 18756460
Gene_locus related to this paper: 9sphi-b6vqa9

Title : Genetic Analysis and Characterization of Poly(aspartic acid) Hydrolase-1 from Sphingomonas sp. KT-1 - Hiraishi_2003_Biomacromolecules_4_80
Author(s) : Hiraishi T , Kajiyama M , Tabata K , Yamato I , Doi Y
Ref : Biomacromolecules , 4 :80 , 2003
Abstract :
PubMedSearch : Hiraishi_2003_Biomacromolecules_4_80
PubMedID: 12523851
Gene_locus related to this paper: 9sphn-q7wsc1