Kues U

References (12)

Title : Evolution of novel wood decay mechanisms in Agaricales revealed by the genome sequences of Fistulina hepatica and Cylindrobasidium torrendii - Floudas_2015_Fungal.Genet.Biol_76_78
Author(s) : Floudas D , Held BW , Riley R , Nagy LG , Koehler G , Ransdell AS , Younus H , Chow J , Chiniquy J , Lipzen A , Tritt A , Sun H , Haridas S , LaButti K , Ohm RA , Kues U , Blanchette RA , Grigoriev IV , Minto RE , Hibbett DS
Ref : Fungal Genet Biol , 76 :78 , 2015
Abstract : Wood decay mechanisms in Agaricomycotina have been traditionally separated in two categories termed white and brown rot. Recently the accuracy of such a dichotomy has been questioned. Here, we present the genome sequences of the white-rot fungus Cylindrobasidium torrendii and the brown-rot fungus Fistulina hepatica both members of Agaricales, combining comparative genomics and wood decay experiments. C. torrendii is closely related to the white-rot root pathogen Armillaria mellea, while F. hepatica is related to Schizophyllum commune, which has been reported to cause white rot. Our results suggest that C. torrendii and S. commune are intermediate between white-rot and brown-rot fungi, but at the same time they show characteristics of decay that resembles soft rot. Both species cause weak wood decay and degrade all wood components but leave the middle lamella intact. Their gene content related to lignin degradation is reduced, similar to brown-rot fungi, but both have maintained a rich array of genes related to carbohydrate degradation, similar to white-rot fungi. These characteristics appear to have evolved from white-rot ancestors with stronger ligninolytic ability. F. hepatica shows characteristics of brown rot both in terms of wood decay genes found in its genome and the decay that it causes. However, genes related to cellulose degradation are still present, which is a plesiomorphic characteristic shared with its white-rot ancestors. Four wood degradation-related genes, homologs of which are frequently lost in brown-rot fungi, show signs of pseudogenization in the genome of F. hepatica. These results suggest that transition toward a brown-rot lifestyle could be an ongoing process in F. hepatica. Our results reinforce the idea that wood decay mechanisms are more diverse than initially thought and that the dichotomous separation of wood decay mechanisms in Agaricomycotina into white rot and brown rot should be revisited.
ESTHER : Floudas_2015_Fungal.Genet.Biol_76_78
PubMedSearch : Floudas_2015_Fungal.Genet.Biol_76_78
PubMedID: 25683379
Gene_locus related to this paper: 9agar-a0a0d6zyq5 , 9agar-a0a0d7a2p9 , 9agar-a0a0d7a2v2 , 9agar-a0a0d7abt2 , 9agar-a0a0d7acd3 , 9agar-a0a0d7acx0 , 9agar-a0a0d7acx9 , 9agar-a0a0d7adg2 , 9agar-a0a0d7a6d0 , 9agar-a0a0d7aen7 , 9agar-a0a0d7aez7 , 9agar-a0a0d7ahq5 , 9agar-a0a0d7akr6 , 9agar-a0a0d7al29 , 9agar-a0a0d7an16 , 9agar-a0a0d7ann7 , 9agar-a0a0d7anv1 , 9homo-a0a0d7atv2 , 9homo-a0a0d7ay28 , 9homo-a0a0d7ayz7 , 9homo-a0a0d7b1w8 , 9homo-a0a0d7b2p0 , 9homo-a0a0d7b4n4 , 9homo-a0a0d7b624 , 9homo-a0a0d7b7r3 , 9homo-a0a0d7b7w3 , 9homo-a0a0d7bac5 , 9homo-a0a0d7bav7 , 9homo-a0a0d7bbx7 , 9homo-a0a0d7bdn7 , 9homo-a0a0d7bgj9 , 9homo-a0a0d7biw2 , 9homo-a0a0d7bqi1 , 9homo-a0a0d7bv80 , 9agar-a0a0d7b6f6 , 9agar-a0a0d7b976 , 9agar-a0a0d7aeu9 , 9agar-a0a0d7ag53 , 9agar-a0a0d7b8a5

Title : Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood - Hori_2014_PLoS.Genet_10_e1004759
Author(s) : Hori C , Ishida T , Igarashi K , Samejima M , Suzuki H , Master E , Ferreira P , Ruiz-Duenas FJ , Held B , Canessa P , Larrondo LF , Schmoll M , Druzhinina IS , Kubicek CP , Gaskell JA , Kersten P , St John F , Glasner J , Sabat G , Splinter BonDurant S , Syed K , Yadav J , Mgbeahuruike AC , Kovalchuk A , Asiegbu FO , Lackner G , Hoffmeister D , Rencoret J , Gutierrez A , Sun H , Lindquist E , Barry K , Riley R , Grigoriev IV , Henrissat B , Kues U , Berka RM , Martinez AT , Covert SF , Blanchette RA , Cullen D
Ref : PLoS Genet , 10 :e1004759 , 2014
Abstract : Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset of these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable of colonizing freshly exposed conifer sapwood despite its high content of extractives, which retards the establishment of other fungal species. The mechanism(s) by which P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report the annotated P. gigantea genome and compare profiles of its transcriptome and secretome when cultured on fresh-cut versus solvent-extracted loblolly pine wood. The P. gigantea genome contains a conventional repertoire of hydrolase genes involved in cellulose/hemicellulose degradation, whose patterns of expression were relatively unperturbed by the absence of extractives. The expression of genes typically ascribed to lignin degradation was also largely unaffected. In contrast, genes likely involved in the transformation and detoxification of wood extractives were highly induced in its presence. Their products included an ABC transporter, lipases, cytochrome P450s, glutathione S-transferase and aldehyde dehydrogenase. Other regulated genes of unknown function and several constitutively expressed genes are also likely involved in P. gigantea's extractives metabolism. These results contribute to our fundamental understanding of pioneer colonization of conifer wood and provide insight into the diverse chemistries employed by fungi in carbon cycling processes.
ESTHER : Hori_2014_PLoS.Genet_10_e1004759
PubMedSearch : Hori_2014_PLoS.Genet_10_e1004759
PubMedID: 25474575
Gene_locus related to this paper: phlgi-a0a0c3nds0 , phlgi-a0a0c3niq6 , phlgi-a0a0c3pc91 , phlgi-a0a0c3pv58 , phlgi-a0a0c3rra0 , phlgi-a0a0c3rvc4 , phlgi-a0a0c3rvu0 , phlgi-a0a0c3s394 , phlgi-a0a0c3s606 , phlgi-a0a0c3s673 , phlgi-a0a0c3s8d3 , phlgi-a0a0c3sce4 , phlgi-a0a0c3sdt8

Title : Genome sequence of the button mushroom Agaricus bisporus reveals mechanisms governing adaptation to a humic-rich ecological niche - Morin_2012_Proc.Natl.Acad.Sci.U.S.A_109_17501
Author(s) : Morin E , Kohler A , Baker AR , Foulongne-Oriol M , Lombard V , Nagy LG , Ohm RA , Patyshakuliyeva A , Brun A , Aerts AL , Bailey AM , Billette C , Coutinho PM , Deakin G , Doddapaneni H , Floudas D , Grimwood J , Hilden K , Kues U , LaButti KM , Lapidus A , Lindquist EA , Lucas SM , Murat C , Riley RW , Salamov AA , Schmutz J , Subramanian V , Wosten HA , Xu J , Eastwood DC , Foster GD , Sonnenberg AS , Cullen D , de Vries RP , Lundell T , Hibbett DS , Henrissat B , Burton KS , Kerrigan RW , Challen MP , Grigoriev IV , Martin F
Ref : Proc Natl Acad Sci U S A , 109 :17501 , 2012
Abstract : Agaricus bisporus is the model fungus for the adaptation, persistence, and growth in the humic-rich leaf-litter environment. Aside from its ecological role, A. bisporus has been an important component of the human diet for over 200 y and worldwide cultivation of the "button mushroom" forms a multibillion dollar industry. We present two A. bisporus genomes, their gene repertoires and transcript profiles on compost and during mushroom formation. The genomes encode a full repertoire of polysaccharide-degrading enzymes similar to that of wood-decayers. Comparative transcriptomics of mycelium grown on defined medium, casing-soil, and compost revealed genes encoding enzymes involved in xylan, cellulose, pectin, and protein degradation are more highly expressed in compost. The striking expansion of heme-thiolate peroxidases and beta-etherases is distinctive from Agaricomycotina wood-decayers and suggests a broad attack on decaying lignin and related metabolites found in humic acid-rich environment. Similarly, up-regulation of these genes together with a lignolytic manganese peroxidase, multiple copper radical oxidases, and cytochrome P450s is consistent with challenges posed by complex humic-rich substrates. The gene repertoire and expression of hydrolytic enzymes in A. bisporus is substantially different from the taxonomically related ectomycorrhizal symbiont Laccaria bicolor. A common promoter motif was also identified in genes very highly expressed in humic-rich substrates. These observations reveal genetic and enzymatic mechanisms governing adaptation to the humic-rich ecological niche formed during plant degradation, further defining the critical role such fungi contribute to soil structure and carbon sequestration in terrestrial ecosystems. Genome sequence will expedite mushroom breeding for improved agronomic characteristics.
ESTHER : Morin_2012_Proc.Natl.Acad.Sci.U.S.A_109_17501
PubMedSearch : Morin_2012_Proc.Natl.Acad.Sci.U.S.A_109_17501
PubMedID: 23045686
Gene_locus related to this paper: agabu-k5x1b4 , agabu-k5x521 , agabu-k5w389 , agabu-k5wbk9 , agabu-k5wrh0 , agabu-k5ws85 , agabu-k5wsf9 , agabu-k5wxv1 , agabu-k5x0d9 , agabu-k5x588 , agabu-k5x5x2 , agabu-k5xd51 , agabu-k5xh54 , agabu-k5xsm1 , agabu-k5xsp8 , agabu-k5xtc1 , agabu-k5y2v2 , agabb-k9i3g9 , agabb-k9hnv7 , agabb-k9hr46 , agabu-k5wys0

Title : The Paleozoic origin of enzymatic lignin decomposition reconstructed from 31 fungal genomes - Floudas_2012_Science_336_1715
Author(s) : Floudas D , Binder M , Riley R , Barry K , Blanchette RA , Henrissat B , Martinez AT , Otillar R , Spatafora JW , Yadav JS , Aerts A , Benoit I , Boyd A , Carlson A , Copeland A , Coutinho PM , de Vries RP , Ferreira P , Findley K , Foster B , Gaskell J , Glotzer D , Gorecki P , Heitman J , Hesse C , Hori C , Igarashi K , Jurgens JA , Kallen N , Kersten P , Kohler A , Kues U , Kumar TK , Kuo A , LaButti K , Larrondo LF , Lindquist E , Ling A , Lombard V , Lucas S , Lundell T , Martin R , McLaughlin DJ , Morgenstern I , Morin E , Murat C , Nagy LG , Nolan M , Ohm RA , Patyshakuliyeva A , Rokas A , Ruiz-Duenas FJ , Sabat G , Salamov A , Samejima M , Schmutz J , Slot JC , St John F , Stenlid J , Sun H , Sun S , Syed K , Tsang A , Wiebenga A , Young D , Pisabarro A , Eastwood DC , Martin F , Cullen D , Grigoriev IV , Hibbett DS
Ref : Science , 336 :1715 , 2012
Abstract : Wood is a major pool of organic carbon that is highly resistant to decay, owing largely to the presence of lignin. The only organisms capable of substantial lignin decay are white rot fungi in the Agaricomycetes, which also contains non-lignin-degrading brown rot and ectomycorrhizal species. Comparative analyses of 31 fungal genomes (12 generated for this study) suggest that lignin-degrading peroxidases expanded in the lineage leading to the ancestor of the Agaricomycetes, which is reconstructed as a white rot species, and then contracted in parallel lineages leading to brown rot and mycorrhizal species. Molecular clock analyses suggest that the origin of lignin degradation might have coincided with the sharp decrease in the rate of organic carbon burial around the end of the Carboniferous period.
ESTHER : Floudas_2012_Science_336_1715
PubMedSearch : Floudas_2012_Science_336_1715
PubMedID: 22745431
Gene_locus related to this paper: aurde-j0d098 , aurde-j0dc31 , glota-s7rlc1 , fompi-s8f7s4 , dacsp-m5fpg2 , dicsq-r7sm16 , dacsp-m5g7q5 , dacsp-m5fr12 , glota-s7q5w3 , fompi-s8f826.1 , fompi-s8f826.2 , dicsq-r7sy09 , glota-s7rt87 , dicsq-r7t032 , glota-s7rym7 , fompi-s8fiv2 , dacsp-m5gda3.2 , dicsq-r7swi6 , dacsp-m5frf2 , fompi-s8ebb6 , dicsq-r7sln3 , dicsq-r7sya6 , dacsp-m5g7g1 , dicsq-r7syx7 , dicsq-r7sx57 , dacsp-m5fps7 , glota-s7pwi7 , dicsq-r7swj6 , fompi-s8ejq6 , dicsq-r7spc3 , glota-s7q258 , dacsp-m5ft65 , glota-s7q3m7 , fompi-s8dkc7 , glota-s7q1z1 , fompi-s8eqi2 , glota-s7q1z8 , fompi-s8du50 , dacsp-m5gg33 , dacsp-m5g3a7 , fompi-s8ecd7 , fompi-s8dps1 , dacsp-m5fwr0 , dicsq-r7sub7 , glota-s7q8k9 , fompi-s8ffc3 , dacsp-m5g2f9 , fompi-s8ecc2 , dacsp-m5g868 , fompi-s8f890 , dicsq-r7t1a8 , fompi-s8ebx4 , fompi-s8eb97 , glota-s7q222 , glota-s7puf0 , fompi-s8f6v9 , dacsp-m5g0z2 , dacsp-m5gdh9 , fompi-s8fb37 , dacsp-m5fy91 , glota-s7q5v6 , fompi-s8fl44 , dicsq-r7stv9 , dicsq-r7szk3 , fompi-s8epq9 , glota-s7rh56 , dacsp-m5gbt1 , punst-r7s3x9 , punst-r7s0t5 , glota-s7q312 , glota-s7rhh6 , dicsq-r7t117 , dicsq-r7slz3

Title : Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis - Fernandez-Fueyo_2012_Proc.Natl.Acad.Sci.U.S.A_109_5458
Author(s) : Fernandez-Fueyo E , Ruiz-Duenas FJ , Ferreira P , Floudas D , Hibbett DS , Canessa P , Larrondo LF , James TY , Seelenfreund D , Lobos S , Polanco R , Tello M , Honda Y , Watanabe T , Ryu JS , Kubicek CP , Schmoll M , Gaskell J , Hammel KE , St John FJ , Vanden Wymelenberg A , Sabat G , Splinter BonDurant S , Syed K , Yadav JS , Doddapaneni H , Subramanian V , Lavin JL , Oguiza JA , Perez G , Pisabarro AG , Ramirez L , Santoyo F , Master E , Coutinho PM , Henrissat B , Lombard V , Magnuson JK , Kues U , Hori C , Igarashi K , Samejima M , Held BW , Barry KW , LaButti KM , Lapidus A , Lindquist EA , Lucas SM , Riley R , Salamov AA , Hoffmeister D , Schwenk D , Hadar Y , Yarden O , de Vries RP , Wiebenga A , Stenlid J , Eastwood D , Grigoriev IV , Berka RM , Blanchette RA , Kersten P , Martinez AT , Vicuna R , Cullen D
Ref : Proc Natl Acad Sci U S A , 109 :5458 , 2012
Abstract : Efficient lignin depolymerization is unique to the wood decay basidiomycetes, collectively referred to as white rot fungi. Phanerochaete chrysosporium simultaneously degrades lignin and cellulose, whereas the closely related species, Ceriporiopsis subvermispora, also depolymerizes lignin but may do so with relatively little cellulose degradation. To investigate the basis for selective ligninolysis, we conducted comparative genome analysis of C. subvermispora and P. chrysosporium. Genes encoding manganese peroxidase numbered 13 and five in C. subvermispora and P. chrysosporium, respectively. In addition, the C. subvermispora genome contains at least seven genes predicted to encode laccases, whereas the P. chrysosporium genome contains none. We also observed expansion of the number of C. subvermispora desaturase-encoding genes putatively involved in lipid metabolism. Microarray-based transcriptome analysis showed substantial up-regulation of several desaturase and MnP genes in wood-containing medium. MS identified MnP proteins in C. subvermispora culture filtrates, but none in P. chrysosporium cultures. These results support the importance of MnP and a lignin degradation mechanism whereby cleavage of the dominant nonphenolic structures is mediated by lipid peroxidation products. Two C. subvermispora genes were predicted to encode peroxidases structurally similar to P. chrysosporium lignin peroxidase and, following heterologous expression in Escherichia coli, the enzymes were shown to oxidize high redox potential substrates, but not Mn(2+). Apart from oxidative lignin degradation, we also examined cellulolytic and hemicellulolytic systems in both fungi. In summary, the C. subvermispora genetic inventory and expression patterns exhibit increased oxidoreductase potential and diminished cellulolytic capability relative to P. chrysosporium.
ESTHER : Fernandez-Fueyo_2012_Proc.Natl.Acad.Sci.U.S.A_109_5458
PubMedSearch : Fernandez-Fueyo_2012_Proc.Natl.Acad.Sci.U.S.A_109_5458
PubMedID: 22434909
Gene_locus related to this paper: cers8-m2r3x2 , cers8-m2qf37 , cers8-m2pcy7 , cers8-m2pcz3 , cers8-m2qn26 , cers8-m2r654 , cers8-m2r8g9 , cers8-m2ps90 , cers8-m2qn44 , cers8-m2q837 , cers8-m2pjy6 , cers8-m2r609 , cers8-m2qy35 , cers8-m2r1n1 , cers8-m2rl22 , cers8-m2qkx5 , cers8-m2qib7 , cers8-m2rgs8 , cers8-m2rlx6 , cers8-m2r4p3 , cers8-m2rf62 , cers8-m2qyx5 , cers8-m2pcz2 , cers8-m2rm22 , cers8-m2qwb7 , cers8-m2r9u3 , cers8-m2pp23 , cers8-m2r613 , cers8-m2rup8 , cers8-m2piv7 , cers8-m2rch3 , cers8-m2qvf7 , cers8-m2qvb7 , cers8-m2qvb2 , cers8-m2pip7 , cers8-m2rb73 , cers8-m2qgd3 , cers8-m2rcg8 , cers8-m2rb68

Title : Insight into trade-off between wood decay and parasitism from the genome of a fungal forest pathogen - Olson_2012_New.Phytol_194_1001
Author(s) : Olson A , Aerts A , Asiegbu F , Belbahri L , Bouzid O , Broberg A , Canback B , Coutinho PM , Cullen D , Dalman K , Deflorio G , van Diepen LT , Dunand C , Duplessis S , Durling M , Gonthier P , Grimwood J , Fossdal CG , Hansson D , Henrissat B , Hietala A , Himmelstrand K , Hoffmeister D , Hogberg N , James TY , Karlsson M , Kohler A , Kues U , Lee YH , Lin YC , Lind M , Lindquist E , Lombard V , Lucas S , Lunden K , Morin E , Murat C , Park J , Raffaello T , Rouze P , Salamov A , Schmutz J , Solheim H , Stahlberg J , Velez H , de Vries RP , Wiebenga A , Woodward S , Yakovlev I , Garbelotto M , Martin F , Grigoriev IV , Stenlid J
Ref : New Phytol , 194 :1001 , 2012
Abstract : Parasitism and saprotrophic wood decay are two fungal strategies fundamental for succession and nutrient cycling in forest ecosystems. An opportunity to assess the trade-off between these strategies is provided by the forest pathogen and wood decayer Heterobasidion annosum sensu lato. We report the annotated genome sequence and transcript profiling, as well as the quantitative trait loci mapping, of one member of the species complex: H. irregulare. Quantitative trait loci critical for pathogenicity, and rich in transposable elements, orphan and secreted genes, were identified. A wide range of cellulose-degrading enzymes are expressed during wood decay. By contrast, pathogenic interaction between H. irregulare and pine engages fewer carbohydrate-active enzymes, but involves an increase in pectinolytic enzymes, transcription modules for oxidative stress and secondary metabolite production. Our results show a trade-off in terms of constrained carbohydrate decomposition and membrane transport capacity during interaction with living hosts. Our findings establish that saprotrophic wood decay and necrotrophic parasitism involve two distinct, yet overlapping, processes.
ESTHER : Olson_2012_New.Phytol_194_1001
PubMedSearch : Olson_2012_New.Phytol_194_1001
PubMedID: 22463738
Gene_locus related to this paper: 9homo-w4jrb9 , 9homo-w4jsg4 , 9homo-w4kds7 , 9homo-w4jwl9 , 9homo-w4kjy2 , 9homo-w4jw43 , 9homo-w4ka20 , 9homo-w4k8t3 , 9homo-w4jz43 , 9homo-w4k8q2 , 9homo-w4k910 , 9homo-w4k6f5 , 9homo-w4k6j3 , 9homo-w4k8n2 , 9homo-w4jrf3 , 9homo-w4ke07 , 9homo-w4k3i8 , 9homo-w4jqh1 , 9agam-w4k203 , 9agam-w4jpy3 , 9agam-w4jn81 , 9agam-w4jmz2

Title : The plant cell wall-decomposing machinery underlies the functional diversity of forest fungi - Eastwood_2011_Science_333_762
Author(s) : Eastwood DC , Floudas D , Binder M , Majcherczyk A , Schneider P , Aerts A , Asiegbu FO , Baker SE , Barry K , Bendiksby M , Blumentritt M , Coutinho PM , Cullen D , de Vries RP , Gathman A , Goodell B , Henrissat B , Ihrmark K , Kauserud H , Kohler A , LaButti K , Lapidus A , Lavin JL , Lee YH , Lindquist E , Lilly W , Lucas S , Morin E , Murat C , Oguiza JA , Park J , Pisabarro AG , Riley R , Rosling A , Salamov A , Schmidt O , Schmutz J , Skrede I , Stenlid J , Wiebenga A , Xie X , Kues U , Hibbett DS , Hoffmeister D , Hogberg N , Martin F , Grigoriev IV , Watkinson SC
Ref : Science , 333 :762 , 2011
Abstract : Brown rot decay removes cellulose and hemicellulose from wood--residual lignin contributing up to 30% of forest soil carbon--and is derived from an ancestral white rot saprotrophy in which both lignin and cellulose are decomposed. Comparative and functional genomics of the "dry rot" fungus Serpula lacrymans, derived from forest ancestors, demonstrated that the evolution of both ectomycorrhizal biotrophy and brown rot saprotrophy were accompanied by reductions and losses in specific protein families, suggesting adaptation to an intercellular interaction with plant tissue. Transcriptome and proteome analysis also identified differences in wood decomposition in S. lacrymans relative to the brown rot Postia placenta. Furthermore, fungal nutritional mode diversification suggests that the boreal forest biome originated via genetic coevolution of above- and below-ground biota.
ESTHER : Eastwood_2011_Science_333_762
PubMedSearch : Eastwood_2011_Science_333_762
PubMedID: 21764756
Gene_locus related to this paper: serl3-f8prj2 , serl3-f8qcc4 , serl9-f8ngp6 , serl9-f8nhd7 , serl9-f8nhq9 , serl9-f8nq77 , serl9-f8nr67 , serl9-f8nrt5 , serl9-f8nvy7.1 , serl9-f8nvy7.2 , serl9-f8nvy8 , serl9-f8nxt0.1 , serl9-f8nxt0.2 , serl9-f8nzr3 , serl9-f8p0f0 , serl9-f8p6v0 , serl9-f8p015 , serl9-f8p018 , serl9-f8p386 , serl9-f8paz8 , serl9-f8pbv1 , serl9-f8pby1 , serl9-f8pc25 , serl9-f8pc39 , serl9-f8nia7 , serl3-f8pju2 , serl9-f8peh1 , serl9-nps3

Title : Obligate biotrophy features unraveled by the genomic analysis of rust fungi - Duplessis_2011_Proc.Natl.Acad.Sci.U.S.A_108_9166
Author(s) : Duplessis S , Cuomo CA , Lin YC , Aerts A , Tisserant E , Veneault-Fourrey C , Joly DL , Hacquard S , Amselem J , Cantarel BL , Chiu R , Coutinho PM , Feau N , Field M , Frey P , Gelhaye E , Goldberg J , Grabherr MG , Kodira CD , Kohler A , Kues U , Lindquist EA , Lucas SM , Mago R , Mauceli E , Morin E , Murat C , Pangilinan JL , Park R , Pearson M , Quesneville H , Rouhier N , Sakthikumar S , Salamov AA , Schmutz J , Selles B , Shapiro H , Tanguay P , Tuskan GA , Henrissat B , Van de Peer Y , Rouze P , Ellis JG , Dodds PN , Schein JE , Zhong S , Hamelin RC , Grigoriev IV , Szabo LJ , Martin F
Ref : Proc Natl Acad Sci U S A , 108 :9166 , 2011
Abstract : Rust fungi are some of the most devastating pathogens of crop plants. They are obligate biotrophs, which extract nutrients only from living plant tissues and cannot grow apart from their hosts. Their lifestyle has slowed the dissection of molecular mechanisms underlying host invasion and avoidance or suppression of plant innate immunity. We sequenced the 101-Mb genome of Melampsora larici-populina, the causal agent of poplar leaf rust, and the 89-Mb genome of Puccinia graminis f. sp. tritici, the causal agent of wheat and barley stem rust. We then compared the 16,399 predicted proteins of M. larici-populina with the 17,773 predicted proteins of P. graminis f. sp tritici. Genomic features related to their obligate biotrophic lifestyle include expanded lineage-specific gene families, a large repertoire of effector-like small secreted proteins, impaired nitrogen and sulfur assimilation pathways, and expanded families of amino acid and oligopeptide membrane transporters. The dramatic up-regulation of transcripts coding for small secreted proteins, secreted hydrolytic enzymes, and transporters in planta suggests that they play a role in host infection and nutrient acquisition. Some of these genomic hallmarks are mirrored in the genomes of other microbial eukaryotes that have independently evolved to infect plants, indicating convergent adaptation to a biotrophic existence inside plant cells.
ESTHER : Duplessis_2011_Proc.Natl.Acad.Sci.U.S.A_108_9166
PubMedSearch : Duplessis_2011_Proc.Natl.Acad.Sci.U.S.A_108_9166
PubMedID: 21536894
Gene_locus related to this paper: pucgt-e3k840 , pucgt-e3kaq6 , pucgt-e3kw59 , pucgt-e3kz16 , pucgt-e3l9v6 , pucgt-e3l279 , pucgt-h6qt25 , mellp-f4reh4 , mellp-f4rhc8 , mellp-f4reh2 , mellp-f4r3y0 , mellp-f4rz15 , mellp-f4rz64 , mellp-f4rl14 , mellp-f4rz66 , mellp-f4s751 , mellp-f4s2g6 , pucgt-e3l1z7 , pucgt-e3l803 , pucgt-e3kst2 , pucgt-e3kst5 , mellp-f4ru03 , pucgt-e3l1z8 , pucgt-e3ktz7 , pucgt-e3jun4 , mellp-f4rl65 , mellp-f4rz16 , mellp-f4ru02 , mellp-f4sav4 , mellp-f4sav3 , mellp-f4s1j0 , mellp-f4rkp0 , mellp-f4s483 , pucgt-e3kzu5 , pucgt-h6qtq8 , mellp-f4r5l5 , pucgt-e3krw7 , pucgt-e3l7w5 , pucgt-e3k2w6 , pucgt-e3kfg2 , pucgt-kex1

Title : Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus) - Stajich_2010_Proc.Natl.Acad.Sci.U.S.A_107_11889
Author(s) : Stajich JE , Wilke SK , Ahren D , Au CH , Birren BW , Borodovsky M , Burns C , Canback B , Casselton LA , Cheng CK , Deng J , Dietrich FS , Fargo DC , Farman ML , Gathman AC , Goldberg J , Guigo R , Hoegger PJ , Hooker JB , Huggins A , James TY , Kamada T , Kilaru S , Kodira C , Kues U , Kupfer D , Kwan HS , Lomsadze A , Li W , Lilly WW , Ma LJ , Mackey AJ , Manning G , Martin F , Muraguchi H , Natvig DO , Palmerini H , Ramesh MA , Rehmeyer CJ , Roe BA , Shenoy N , Stanke M , Ter-Hovhannisyan V , Tunlid A , Velagapudi R , Vision TJ , Zeng Q , Zolan ME , Pukkila PJ
Ref : Proc Natl Acad Sci U S A , 107 :11889 , 2010
Abstract : The mushroom Coprinopsis cinerea is a classic experimental model for multicellular development in fungi because it grows on defined media, completes its life cycle in 2 weeks, produces some 10(8) synchronized meiocytes, and can be manipulated at all stages in development by mutation and transformation. The 37-megabase genome of C. cinerea was sequenced and assembled into 13 chromosomes. Meiotic recombination rates vary greatly along the chromosomes, and retrotransposons are absent in large regions of the genome with low levels of meiotic recombination. Single-copy genes with identifiable orthologs in other basidiomycetes are predominant in low-recombination regions of the chromosome. In contrast, paralogous multicopy genes are found in the highly recombining regions, including a large family of protein kinases (FunK1) unique to multicellular fungi. Analyses of P450 and hydrophobin gene families confirmed that local gene duplications drive the expansions of paralogous copies and the expansions occur in independent lineages of Agaricomycotina fungi. Gene-expression patterns from microarrays were used to dissect the transcriptional program of dikaryon formation (mating). Several members of the FunK1 kinase family are differentially regulated during sexual morphogenesis, and coordinate regulation of adjacent duplications is rare. The genomes of C. cinerea and Laccaria bicolor, a symbiotic basidiomycete, share extensive regions of synteny. The largest syntenic blocks occur in regions with low meiotic recombination rates, no transposable elements, and tight gene spacing, where orthologous single-copy genes are overrepresented. The chromosome assembly of C. cinerea is an essential resource in understanding the evolution of multicellularity in the fungi.
ESTHER : Stajich_2010_Proc.Natl.Acad.Sci.U.S.A_107_11889
PubMedSearch : Stajich_2010_Proc.Natl.Acad.Sci.U.S.A_107_11889
PubMedID: 20547848
Gene_locus related to this paper: copc7-a8n2b8 , copc7-a8n3e0 , copc7-a8n3e1 , copc7-a8n6a5 , copc7-a8n8h4 , copc7-a8n702 , copc7-a8n941 , copc7-a8nkc7 , copc7-a8nll5 , copc7-a8nll6 , copc7-a8nqf4 , copc7-a8nqg3 , copc7-a8nqv8 , copc7-a8nvb5 , copc7-a8nwm2 , copc7-a8nz18 , copc7-a8p0p4 , copc7-d6rlx1 , copc7-d6rnh7 , copc7-kex1 , copci-b9u444 , copc7-a8nb05 , copc7-a8nha0 , copci-b9u443 , copc7-a8nq30 , copc7-a8nh79 , copc7-d6rm78 , copc7-a8nzs7 , copc7-axe1

Title : Genome sequence of the model mushroom Schizophyllum commune - Ohm_2010_Nat.Biotechnol_28_957
Author(s) : Ohm RA , de Jong JF , Lugones LG , Aerts A , Kothe E , Stajich JE , de Vries RP , Record E , Levasseur A , Baker SE , Bartholomew KA , Coutinho PM , Erdmann S , Fowler TJ , Gathman AC , Lombard V , Henrissat B , Knabe N , Kues U , Lilly WW , Lindquist E , Lucas S , Magnuson JK , Piumi F , Raudaskoski M , Salamov A , Schmutz J , Schwarze FW , vanKuyk PA , Horton JS , Grigoriev IV , Wosten HA
Ref : Nat Biotechnol , 28 :957 , 2010
Abstract : Much remains to be learned about the biology of mushroom-forming fungi, which are an important source of food, secondary metabolites and industrial enzymes. The wood-degrading fungus Schizophyllum commune is both a genetically tractable model for studying mushroom development and a likely source of enzymes capable of efficient degradation of lignocellulosic biomass. Comparative analyses of its 38.5-megabase genome, which encodes 13,210 predicted genes, reveal the species's unique wood-degrading machinery. One-third of the 471 genes predicted to encode transcription factors are differentially expressed during sexual development of S. commune. Whereas inactivation of one of these, fst4, prevented mushroom formation, inactivation of another, fst3, resulted in more, albeit smaller, mushrooms than in the wild-type fungus. Antisense transcripts may also have a role in the formation of fruiting bodies. Better insight into the mechanisms underlying mushroom formation should affect commercial production of mushrooms and their industrial use for producing enzymes and pharmaceuticals.
ESTHER : Ohm_2010_Nat.Biotechnol_28_957
PubMedSearch : Ohm_2010_Nat.Biotechnol_28_957
PubMedID: 20622885
Gene_locus related to this paper: schcm-d8pqz6 , schcm-d8prj2 , schcm-d8pug6 , schcm-d8pxe8 , schcm-d8pxe9 , schcm-d8pxz1 , schcm-d8q1c7 , schcm-d8q2b4 , schcm-d8q3j1 , schcm-d8q5m5 , schcm-d8q7x7.1 , schcm-d8q7x7.2 , schcm-d8q8y8 , schcm-d8q9n6 , schcm-d8q697 , schcm-d8qip8 , schcm-d8q5s5 , schcm-d8ppb3 , schcm-d8ppb6 , schcm-d8pv73 , schcm-d8pzm1 , schcm-d8q5a7 , schcm-d8qif0

Title : Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion - Martinez_2009_Proc.Natl.Acad.Sci.U.S.A_106_1954
Author(s) : Martinez D , Challacombe J , Morgenstern I , Hibbett D , Schmoll M , Kubicek CP , Ferreira P , Ruiz-Duenas FJ , Martinez AT , Kersten P , Hammel KE , Vanden Wymelenberg A , Gaskell J , Lindquist E , Sabat G , Bondurant SS , Larrondo LF , Canessa P , Vicuna R , Yadav J , Doddapaneni H , Subramanian V , Pisabarro AG , Lavin JL , Oguiza JA , Master E , Henrissat B , Coutinho PM , Harris P , Magnuson JK , Baker SE , Bruno K , Kenealy W , Hoegger PJ , Kues U , Ramaiya P , Lucas S , Salamov A , Shapiro H , Tu H , Chee CL , Misra M , Xie G , Teter S , Yaver D , James T , Mokrejs M , Pospisek M , Grigoriev IV , Brettin T , Rokhsar D , Berka R , Cullen D
Ref : Proc Natl Acad Sci U S A , 106 :1954 , 2009
Abstract : Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome, and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exocellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in medium containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative beta-1-4 endoglucanase were expressed at high levels relative to glucose-grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Also up-regulated during growth on cellulose medium were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H(2)O(2). These observations are consistent with a biodegradative role for Fenton chemistry in which Fe(II) and H(2)O(2) react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. Comparisons with the closely related white-rot fungus Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which the capacity for efficient depolymerization of lignin was lost.
ESTHER : Martinez_2009_Proc.Natl.Acad.Sci.U.S.A_106_1954
PubMedSearch : Martinez_2009_Proc.Natl.Acad.Sci.U.S.A_106_1954
PubMedID: 19193860
Gene_locus related to this paper: pospm-b8p1f3 , pospm-b8p2q7 , pospm-b8p4n0 , pospm-b8p4n9 , pospm-b8p5g9 , pospm-b8p5r9 , pospm-b8p6h2 , pospm-b8p7b1 , pospm-b8p7c4 , pospm-b8p8w7 , pospm-b8p9j1 , pospm-b8p164 , pospm-b8p280 , pospm-b8p423.1 , pospm-b8p423.2 , pospm-b8p858 , pospm-b8pam2 , pospm-b8pam5 , pospm-b8pb68 , pospm-b8pbm3 , pospm-b8pc54 , pospm-b8pc56 , pospm-b8pce4 , pospm-b8pd91 , pospm-b8pdk6 , pospm-b8ph32 , pospm-b8ph43 , pospm-b8phc9 , pospm-b8php7 , pospm-b8phy5 , pospm-b8pjg8 , pospm-b8pji9 , pospm-b8plr5 , pospm-b8pmk3 , pospm-b8pfg0 , pospm-b8pg35 , pospm-b8pa20.1 , pospm-b8pa20.2 , pospm-b8p4g8 , pospm-b8phn6

Title : The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis - Martin_2008_Nature_452_88
Author(s) : Martin F , Aerts A , Ahren D , Brun A , Danchin EG , Duchaussoy F , Gibon J , Kohler A , Lindquist E , Pereda V , Salamov A , Shapiro HJ , Wuyts J , Blaudez D , Buee M , Brokstein P , Canback B , Cohen D , Courty PE , Coutinho PM , Delaruelle C , Detter JC , Deveau A , Difazio S , Duplessis S , Fraissinet-Tachet L , Lucic E , Frey-Klett P , Fourrey C , Feussner I , Gay G , Grimwood J , Hoegger PJ , Jain P , Kilaru S , Labbe J , Lin YC , Legue V , Le Tacon F , Marmeisse R , Melayah D , Montanini B , Muratet M , Nehls U , Niculita-Hirzel H , Oudot-Le Secq MP , Peter M , Quesneville H , Rajashekar B , Reich M , Rouhier N , Schmutz J , Yin T , Chalot M , Henrissat B , Kues U , Lucas S , Van de Peer Y , Podila GK , Polle A , Pukkila PJ , Richardson PM , Rouze P , Sanders IR , Stajich JE , Tunlid A , Tuskan G , Grigoriev IV
Ref : Nature , 452 :88 , 2008
Abstract : Mycorrhizal symbioses--the union of roots and soil fungi--are universal in terrestrial ecosystems and may have been fundamental to land colonization by plants. Boreal, temperate and montane forests all depend on ectomycorrhizae. Identification of the primary factors that regulate symbiotic development and metabolic activity will therefore open the door to understanding the role of ectomycorrhizae in plant development and physiology, allowing the full ecological significance of this symbiosis to be explored. Here we report the genome sequence of the ectomycorrhizal basidiomycete Laccaria bicolor (Fig. 1) and highlight gene sets involved in rhizosphere colonization and symbiosis. This 65-megabase genome assembly contains approximately 20,000 predicted protein-encoding genes and a very large number of transposons and repeated sequences. We detected unexpected genomic features, most notably a battery of effector-type small secreted proteins (SSPs) with unknown function, several of which are only expressed in symbiotic tissues. The most highly expressed SSP accumulates in the proliferating hyphae colonizing the host root. The ectomycorrhizae-specific SSPs probably have a decisive role in the establishment of the symbiosis. The unexpected observation that the genome of L. bicolor lacks carbohydrate-active enzymes involved in degradation of plant cell walls, but maintains the ability to degrade non-plant cell wall polysaccharides, reveals the dual saprotrophic and biotrophic lifestyle of the mycorrhizal fungus that enables it to grow within both soil and living plant roots. The predicted gene inventory of the L. bicolor genome, therefore, points to previously unknown mechanisms of symbiosis operating in biotrophic mycorrhizal fungi. The availability of this genome provides an unparalleled opportunity to develop a deeper understanding of the processes by which symbionts interact with plants within their ecosystem to perform vital functions in the carbon and nitrogen cycles that are fundamental to sustainable plant productivity.
ESTHER : Martin_2008_Nature_452_88
PubMedSearch : Martin_2008_Nature_452_88
PubMedID: 18322534
Gene_locus related to this paper: lacbs-b0cns1 , lacbs-b0cpl4 , lacbs-b0cr62 , lacbs-b0cr66 , lacbs-b0csq9 , lacbs-b0ct56 , lacbs-b0ctt5 , lacbs-b0cuw1 , lacbs-b0cv23 , lacbs-b0cxm7 , lacbs-b0cz37 , lacbs-b0czx3 , lacbs-b0d0z5 , lacbs-b0d4i0 , lacbs-b0d4j3 , lacbs-b0d5n6 , lacbs-b0d8k0 , lacbs-b0d263 , lacbs-b0dhh1 , lacbs-b0dkp6 , lacbs-b0dmr2 , lacbs-b0dmt4 , lacbs-b0dsx5 , lacbs-b0dt05 , lacbs-b0dtw4 , lacbs-b0du88 , lacbs-b0dsl6