Suzuki S

References (28)

Title : Neuronal SAM68 differentially regulates alternative last exon splicing and ensures proper synapse development and function - Darwish_2023_J.Biol.Chem__105168
Author(s) : Darwish M , Ito M , Iijima Y , Takase A , Ayukawa N , Suzuki S , Tanaka M , Komori K , Kaida D , Iijima T
Ref : Journal of Biological Chemistry , :105168 , 2023
Abstract : Alternative splicing in the 3' untranslated region (3'UTR) of mammalian genes plays a crucial role in diverse biological processes, including cell differentiation and development. SAM68 is a key splicing regulator that controls the diversity of 3'UTR isoforms through alternative last exon (ALE) selection. However, the tissue/cell type-specific mechanisms underlying the splicing control at the 3' end and its functional significance remain unclear. Here, we show that SAM68 regulates ALE splicing in a dose-dependent manner and the neuronal splicing is differentially regulated depending on the characteristics of the target transcript. Specifically, we found that SAM68 regulates IL-1 receptor-associated protein (Il1rap) splicing through the interaction with U1 small nuclear ribonucleoprotein (U1 snRNP). In contrast, the ALE splicing of protocadherin-15 (Pcdh15), a gene implicated in several neuropsychiatric disorders, is independent of U1 snRNP but modulated by the CaMK signaling pathway. We found that the aberrant ALE selection of Pcdh15 led to a conversion from a membrane-bound to a soluble isoform and consequently disrupted its localization into excitatory and inhibitory synapses. Notably, the neuronal expression of the soluble form of PCDH15 (sPCDH15) preferentially affected the number of inhibitory synapses. Moreover, the sPCDH15 interacted physically with alpha-neurexins and further disrupted neuroligin-2-induced inhibitory synapses in artificial synapse formation assays. Our findings provide novel insights into the role of neuron-specific alternative 3'UTR isoform selections in synapse development.
ESTHER : Darwish_2023_J.Biol.Chem__105168
PubMedSearch : Darwish_2023_J.Biol.Chem__105168
PubMedID: 37595869

Title : Salivary leukocyte esterase activity by SillHa is a risk indicator of periodontal disease - Ishii_2023_BMC.Oral.Health_23_187
Author(s) : Ishii K , Venkataiah VS , Kajiwara T , Umezawa K , Suzuki S , Nakano M , Sawaguchi M , Yahata Y , Saito M
Ref : BMC Oral Health , 23 :187 , 2023
Abstract : BACKGROUND: There is increasing evidence that diagnostic salivary tests measuring inflammatory biomarkers are being developed to assess inflammatory status for early detection, prevention, and progression of periodontal disease. Therefore, the aim of the present study was to investigate and identify the salivary biomarker that can predict the inflammatory status of periodontal disease. METHODS: A total of 36 patients (28 women and 8 men) with an average age of 57 years were investigated. Unstimulated saliva was collected from the recruited subjects and analyzed using SillHa, a saliva-testing device that measures bacteria count, saliva buffer capacity, acidity, leukocyte esterase, protein, and ammonia. Periodontal parameters were then obtained by clinical examination and initial periodontal therapy was performed. Data obtained with SillHa were compared with clinical periodontal parameters at baseline, re-examination (three months from baseline), and final examination (six months from re-examination). RESULTS: Leukocyte esterase activity in saliva measured by SillHa; BOP and PCR measured by clinical examination showed a significant difference between baseline and final examination and between re-examination and final examination. Patients in the lower median group (group 1) had a significant difference in leukocyte esterase activity between baseline and final examination and re-examination and final examination. In addition, patients in Group 1 had significantly lower BOP between baseline and final examination. While patients in the higher median group (group 2) showed a modest decrease in leukocyte esterase activity, which was significant only between baseline and final examination, no significant changes were observed concerning BOP. Furthermore, the associated systemic disease was observed in 30% and 81.2% of group 1 and 2 patients, respectively. CONCLUSION: The results suggest that leukocyte esterase activity in saliva measured by SillHa could serve as a reliable diagnostic marker for monitoring inflammatory status in periodontal disease.
ESTHER : Ishii_2023_BMC.Oral.Health_23_187
PubMedSearch : Ishii_2023_BMC.Oral.Health_23_187
PubMedID: 36998066

Title : A carlactonoic acid methyltransferase that contributes to the inhibition of shoot branching in Arabidopsis - Mashiguchi_2022_Proc.Natl.Acad.Sci.U.S.A_119_e2111565119
Author(s) : Mashiguchi K , Seto Y , Onozuka Y , Suzuki S , Takemoto K , Wang Y , Dong L , Asami K , Noda R , Kisugi T , Kitaoka N , Akiyama K , Bouwmeester H , Yamaguchi S
Ref : Proc Natl Acad Sci U S A , 119 :e2111565119 , 2022
Abstract : SignificanceStrigolactones (SLs) are a group of apocarotenoid hormones, which regulates shoot branching and other diverse developmental processes in plants. The major bioactive form(s) of SLs as endogenous hormones has not yet been clarified. Here, we identify an Arabidopsis methyltransferase, CLAMT, responsible for the conversion of an inactive precursor to a biologically active SL that can interact with the SL receptor in vitro. Reverse genetic analysis showed that this enzyme plays an essential role in inhibiting shoot branching. This mutant also contributed to specifying the SL-related metabolites that could move from root to shoot in grafting experiments. Our work has identified a key enzyme necessary for the production of the bioactive form(s) of SLs.
ESTHER : Mashiguchi_2022_Proc.Natl.Acad.Sci.U.S.A_119_e2111565119
PubMedSearch : Mashiguchi_2022_Proc.Natl.Acad.Sci.U.S.A_119_e2111565119
PubMedID: 35344437

Title : Urinary titin N-terminal fragment concentration is an indicator of preoperative sarcopenia and nutritional status in patients with gastrointestinal tract and hepatobiliary pancreatic malignancies - Miyoshi_2020_Nutrition_79-80_110957
Author(s) : Miyoshi K , Shimoda M , Udo R , Oshiro Y , Suzuki S
Ref : Nutrition , 79-80 :110957 , 2020
Abstract : OBJECTIVES: Recent reports indicate that preoperative patients with gastrointestinal malignancies often have sarcopenia. The diagnosis of sarcopenia is generally done by evaluation of walking speed, grip strength, and skeletal muscle volume of the limbs on computed tomography (CT). However, these parameters are objective indices, and new indicators for diagnosis, such as molecular biomarkers, have been anticipated. The aim of this study was to investigate whether titin, a muscular contractile protein present in sarcomeres, is an indicator of sarcopenia. METHODS: We analyzed 39 patients with gastrointestinal tract and hepatobiliary pancreatic malignancies who underwent surgery. We compared urinary titin n-terminal fragment concentration (UTF) with clinical factors, subcutaneous fat volume, and skeletal muscle volume index, and also compared UTF levels between patients with and without sarcopenia. RESULTS: The patients comprised 24 men and 15 women, with a mean age of 72 y (range: 35-85 y). Cancer locations were the pancreas (n = 17), liver (n = 9), stomach (n = 5), colorectum (n = 5), and esophagus (n = 3). UTF was significantly higher in patients with sarcopenia (P = 0.04), and showed statistically significant negative correlations with albumin (r = -2.61, P = 0.001), pre-albumin (r = -2.14, P = 0.02), body mass index (r = -0.49, P = 0.007), cholinesterase (r = -0.02, P = 0.01, skeletal muscle volume index (r = -0.16, P = 0.04), and subcutaneous fat volume (r = -0.03, P = 0.007). CONCLUSION: UTF may be a new index for preoperative nutritional assessment in patients with gastrointestinal malignancies.
ESTHER : Miyoshi_2020_Nutrition_79-80_110957
PubMedSearch : Miyoshi_2020_Nutrition_79-80_110957
PubMedID: 32866763

Title : Early administration of galantamine from preplaque phase suppresses oxidative stress and improves cognitive behavior in APPswe\/PS1dE9 mouse model of Alzheimer's disease - Saito_2019_Free.Radic.Biol.Med_145_20
Author(s) : Saito T , Hisahara S , Iwahara N , Emoto MC , Yokokawa K , Suzuki H , Manabe T , Matsumura A , Suzuki S , Matsushita T , Kawamata J , Sato-Akaba H , Fujii HG , Shimohama S
Ref : Free Radic Biol Med , 145 :20 , 2019
Abstract : Alzheimer's disease (AD) is a common neurodegenerative disease that progressively impairs memory and cognition. Deposition of amyloid-beta (Abeta) peptides is the most important pathophysiological hallmark of AD. Oxidative stress induced by generation of reactive oxygen species (ROS) is a prominent phenomenon in AD and known to occur early in the course of AD. Several reports suggest a relationship between change in redox status and AD pathology including progressive Abeta deposition, glial cell activation, and inflammation. Galantamine is an acetylcholinesterase inhibitor and has been reported to have an oxidative stress inhibitory function. In the present study, galantamine was administered orally to AD model mice from before the appearance of Abeta plaques (preplaque phase), and in vivo change in redox status of the brain was measured using electron paramagnetic resonance (EPR) imaging. Administration of galantamine from the preplaque phase ameliorated memory decline in Morris water maze test and novel object recognition test. Monitoring of the redox status of the brain using EPR imaging showed that galantamine treatment improved the unbalanced redox state. Additionally, galantamine administration enhanced microglial function to promote Abeta clearance, reducing the Abeta-positive area in the cortex and amount of insoluble Abeta in the brain. In contrast, galantamine treatment from the preplaque phase suppressed the production of proinflammatory cytokines through neurotoxic microglial activity. Therefore, galantamine administration from the preplaque phase may have the potential of clinical application for the prevention of AD. In addition, our results demonstrate the usefulness of EPR imaging for speedy and quantitative evaluation of the efficacy of disease-modifying drugs for AD.
ESTHER : Saito_2019_Free.Radic.Biol.Med_145_20
PubMedSearch : Saito_2019_Free.Radic.Biol.Med_145_20
PubMedID: 31536772

Title : Raphidocelis subcapitata (=Pseudokirchneriella subcapitata) provides an insight into genome evolution and environmental adaptations in the Sphaeropleales - Suzuki_2018_Sci.Rep_8_8058
Author(s) : Suzuki S , Yamaguchi H , Nakajima N , Kawachi M
Ref : Sci Rep , 8 :8058 , 2018
Abstract : The Sphaeropleales are a dominant group of green algae, which contain species important to freshwater ecosystems and those that have potential applied usages. In particular, Raphidocelis subcapitata is widely used worldwide for bioassays in toxicological risk assessments. However, there are few comparative genome analyses of the Sphaeropleales. To reveal genome evolution in the Sphaeropleales based on well-resolved phylogenetic relationships, nuclear, mitochondrial, and plastid genomes were sequenced in this study. The plastid genome provides insights into the phylogenetic relationships of R. subcapitata, which is located in the most basal lineage of the four species in the family Selenastraceae. The mitochondrial genome shows dynamic evolutionary histories with intron expansion in the Selenastraceae. The 51.2 Mbp nuclear genome of R. subcapitata, encoding 13,383 protein-coding genes, is more compact than the genome of its closely related oil-rich species, Monoraphidium neglectum (Selenastraceae), Tetradesmus obliquus (Scenedesmaceae), and Chromochloris zofingiensis (Chromochloridaceae); however, the four species share most of their genes. The Sphaeropleales possess a large number of genes for glycerolipid metabolism and sugar assimilation, which suggests that this order is capable of both heterotrophic and mixotrophic lifestyles in nature. Comparison of transporter genes suggests that the Sphaeropleales can adapt to different natural environmental conditions, such as salinity and low metal concentrations.
ESTHER : Suzuki_2018_Sci.Rep_8_8058
PubMedSearch : Suzuki_2018_Sci.Rep_8_8058
PubMedID: 29795299
Gene_locus related to this paper: 9chlo-a0a2v0p934 , 9chlo-a0a2v0nyk8

Title : Neuroligin-induced presynaptic differentiation through SLM2-mediated splicing modifications of neurexin in cerebellar cultures - Sato_2017_Biochem.Biophys.Res.Commun_493_1030
Author(s) : Sato Y , Suzuki S , Iijima Y , Iijima T
Ref : Biochemical & Biophysical Research Communications , 493 :1030 , 2017
Abstract : Neurexins (NRXs) and neuroligins (NLs) play important roles in synapse specification. The alternatively spliced segment 4 (AS4) of NRX genes (Nrxn) is a critical element in selective trans-synaptic interactions. However, the role of splicing of NRXs and NLs in synapse specification is not fully understood. To investigate the exact role of splice-dependent NRX-NL interaction in the specification of glutamatergic and gamma-aminobutyric acid (GABA)-ergic synapses in the cerebellum, we evaluated the synaptogenic receptor activity of NL1/2/3 isoforms in a neuron-fibroblast co-culture system, in which the Nrxn AS4 segments are manipulated using SLM2, a selective and dominant regulator of AS4 splicing. We show that ectopic SLM2 expression (SLM2 E/E) causes marked skipping of exon 20 of AS4 in cerebellar neuron culture. Whereas NLs can induce VAMP2(+) presynaptic contacts from mainly glutamatergic neurons in both uninfected (control) and SLM2 E/E co-cultures, they induce VGAT(+) GABAergic contacts in the control culture, but not properly in the SLM2 E/E culture. Furthermore, Nrxn3 is responsible for the NL-induced assembly of GABAergic synapses in co-culture. Importantly, lentivirus-based expression of Nrxn3 containing exon 20 restores the reduced NL-induced GABAergic contacts in the SLM2 E/E co-culture. Therefore, our findings may provide further insights into NRX-NL mediated synapse specification.
ESTHER : Sato_2017_Biochem.Biophys.Res.Commun_493_1030
PubMedSearch : Sato_2017_Biochem.Biophys.Res.Commun_493_1030
PubMedID: 28939043

Title : DPP4 Inhibition Ameliorates Cardiac Function by Blocking the Cleavage of HMGB1 in Diabetic Mice After Myocardial Infarction - Sato_2017_Int.Heart.J_58_778
Author(s) : Sato A , Suzuki S , Watanabe S , Shimizu T , Nakamura Y , Misaka T , Yokokawa T , Shishido T , Saitoh SI , Ishida T , Kubota I , Takeishi Y
Ref : Int Heart J , 58 :778 , 2017
Abstract : High mobility group box 1 (HMGB1), a ubiquitous DNA-binding protein, promotes angiogenesis and tissue repair, resulting in restored cardiac function after myocardial infarction (MI). Although dipeptidyl peptidase 4 (DPP4) degrades certain peptides, it remains unclear as to whether HMGB1 is a substrate of DPP4 and whether DPP4 inhibition prevents the cleavage of HMGB1.In transgenic mice with cardiac-specific overexpression of HMGB1 (TG) and wild-type mice (WT), a diabetic state was induced by streptozotocin, and MI was created by ligation of the left anterior descending coronary artery. To inhibit DPP4 activity, a DPP4 inhibitor anagliptin was used. The plasma levels of HMGB1, infarct size, echocardiographic data, angiogenesis, and vascular endothelial growth factor (VEGF) expression in the peri-infarct area were compared among non-diabetic MI WT/TG, diabetic MI WT/TG, and anagliptin-treated diabetic MI WT/TG mice.DPP4 activity was increased in the diabetic state and blocked by anagliptin administration. The HMGB1 plasma levels were reduced in the diabetic TG compared with the non-diabetic TG mice, but DPP4 inhibition with anagliptin increased HMGB1 plasma levels in the diabetic TG mice. The infarct area was significantly larger in the diabetic TG than in the non-diabetic TG mice, and it was reduced by DPP4 inhibition. Cardiac function, angiogenesis, and VEGF expression were impaired in the diabetic TG mice, but they were ameliorated by the DPP4 inhibition to levels similar to those found in the non-diabetic TG mice.The DPP4 inhibitor ameliorated cardiac function by inhibiting the inactivation of HMGB1 in diabetic mice after MI.
ESTHER : Sato_2017_Int.Heart.J_58_778
PubMedSearch : Sato_2017_Int.Heart.J_58_778
PubMedID: 28966327

Title : Draft genome sequence of bitter gourd (Momordica charantia), a vegetable and medicinal plant in tropical and subtropical regions - Urasaki_2017_DNA.Res_24_51
Author(s) : Urasaki N , Takagi H , Natsume S , Uemura A , Taniai N , Miyagi N , Fukushima M , Suzuki S , Tarora K , Tamaki M , Sakamoto M , Terauchi R , Matsumura H
Ref : DNA Research , 24 :51 , 2017
Abstract : Bitter gourd (Momordica charantia) is an important vegetable and medicinal plant in tropical and subtropical regions globally. In this study, the draft genome sequence of a monoecious bitter gourd inbred line, OHB3-1, was analyzed. Through Illumina sequencing and de novo assembly, scaffolds of 285.5 Mb in length were generated, corresponding to -84% of the estimated genome size of bitter gourd (339 Mb). In this draft genome sequence, 45,859 protein-coding gene loci were identified, and transposable elements accounted for 15.3% of the whole genome. According to synteny mapping and phylogenetic analysis of conserved genes, bitter gourd was more related to watermelon (Citrullus lanatus) than to cucumber (Cucumis sativus) or melon (C. melo). Using RAD-seq analysis, 1507 marker loci were genotyped in an F2 progeny of two bitter gourd lines, resulting in an improved linkage map, comprising 11 linkage groups. By anchoring RAD tag markers, 255 scaffolds were assigned to the linkage map. Comparative analysis of genome sequences and predicted genes determined that putative trypsin-inhibitor and ribosome-inactivating genes were distinctive in the bitter gourd genome. These genes could characterize the bitter gourd as a medicinal plant.
ESTHER : Urasaki_2017_DNA.Res_24_51
PubMedSearch : Urasaki_2017_DNA.Res_24_51
PubMedID: 28028039
Gene_locus related to this paper: momch-a0a6j1c0s2

Title : Serum cholinesterase is an important prognostic factor in chronic heart failure - Sato_2015_Heart.Vessels_30_204
Author(s) : Sato T , Yamauchi H , Suzuki S , Yoshihisa A , Yamaki T , Sugimoto K , Kunii H , Nakazato K , Suzuki H , Saitoh S , Takeishi Y
Ref : Heart Vessels , 30 :204 , 2015
Abstract : We determine the importance of indicators of nutrition including lymphocyte, total protein, albumin, cholinesterase and body mass index, and compare the prognostic significance in chronic heart failure (CHF). We examined consecutive 465 CHF patients (376 males, age 62 +/- 14 years) who underwent cardiopulmonary exercise testing, echocardiography and blood examination including indicators of nutrition at the same time in our hospital. The patients were followed up [median period 766 days (interquartile range 500-1060)] to register cardiac deaths and rehospitalization due to worsening heart failure. There were 180 cardiac events during the follow-up periods. Patients with cardiac events had lower cholinesterase level than those without events (P < 0.001). On the receiver operating characteristic analysis, the best cut-off value for cholinesterase was 240 U/l (area under the curve 0.720). In the Kaplan-Meier analysis, patients with cholinesterase <240 U/l had significantly higher cardiac event rates than those with cholinesterase >240 U/l. Multivariable Cox proportional hazards model demonstrated that NYHA class III [hazard ratio (HR): 1.688, 95 % confidence interval (CI) 1.062-2.684, P = 0.027], eGFR (HR: 0.983, 95 % CI 0.971-0.995, P = 0.006), sodium concentration (HR: 0.947, 95 % CI 0.897-0.999, P < 0.046), log BNP (HR: 1.880, 95 % CI 1.509-2.341, P < 0.001), cholinesterase (HR: 0.996, 95 % CI 0.993-0.998, P = 0.002) and exertional periodic breathing (HR: 1.619, 95 % CI 1.098-2.388, P = 0.015) were independent factors to predict adverse clinical outcomes. Serum cholinesterase level was an important prognostic factor in CHF.
ESTHER : Sato_2015_Heart.Vessels_30_204
PubMedSearch : Sato_2015_Heart.Vessels_30_204
PubMedID: 24463844

Title : Quality of life in purely ocular myasthenia in Japan - Suzuki_2014_BMC.Neurol_14_142
Author(s) : Suzuki S , Murai H , Imai T , Nagane Y , Masuda M , Tsuda E , Konno S , Oji S , Nakane S , Motomura M , Suzuki N , Utsugisawa K
Ref : BMC Neurol , 14 :142 , 2014
Abstract : BACKGROUND: Since there has been no conclusive evidence regarding the treatment of ocular myasthenia, treatment guidelines were recently issued by the European Federation of Neurological Societies/European Neurological Society (EFNS/ENS). However, the therapeutic outcomes concerning the quality-of-life (QOL) of patients with ocular myasthenia are not yet fully understood.
METHODS: We investigated the therapeutic outcomes of patients with purely ocular myasthenia in a multicenter cross-sectional survey in Japan. To evaluate the severity of ocular symptoms, we used the ocular-quantitative MG (QMG) score advocated by Myasthenia Gravis Foundation of America. We used the Japanese translated version of the MG-QOL15, a self-appraised scoring system.
RESULTS: Of 607 myasthenia gravis (MG) patients with an observation-duration of illness >= 2 years, the cases of 123 patients (20%) were limited to ocular muscles (purely ocular myasthenia). During the entire clinical course, 81 patients experienced both ptosis and diplopia, 36 had ptosis alone, and six had diplopia alone. Acetyl-cholinesterase inhibitors and prednisolone were used in 98 and 52 patients, respectively. Treatment improved ocular symptoms, with the mean reduction in ocular-QMG score of 2.3 +/- 1.8 points. However, 47 patients (38%) failed to gain minimal manifestation or a better status. Patients with unfavorable outcomes also self-reported severe QOL impairment. Multivariate analyses showed that the pretreatment ocular-QMG score was associated with unfavorable outcomes, but not associated with the patient's QOL. CONCLUSION: A treatment strategy designed in accord with a patient's ocular presentation must be considered in order to improve ocular symptoms and the patient's QOL.
ESTHER : Suzuki_2014_BMC.Neurol_14_142
PubMedSearch : Suzuki_2014_BMC.Neurol_14_142
PubMedID: 24996227

Title : Genomic analysis by deep sequencing of the probiotic Lactobacillus brevis KB290 harboring nine plasmids reveals genomic stability - Fukao_2013_PLoS.One_8_e60521
Author(s) : Fukao M , Oshima K , Morita H , Toh H , Suda W , Kim SW , Suzuki S , Yakabe T , Hattori M , Yajima N
Ref : PLoS ONE , 8 :e60521 , 2013
Abstract : We determined the complete genome sequence of Lactobacillus brevis KB290, a probiotic lactic acid bacterium isolated from a traditional Japanese fermented vegetable. The genome contained a 2,395,134-bp chromosome that housed 2,391 protein-coding genes and nine plasmids that together accounted for 191 protein-coding genes. KB290 contained no virulence factor genes, and several genes related to presumptive cell wall-associated polysaccharide biosynthesis and the stress response were present in L. brevis KB290 but not in the closely related L. brevis ATCC 367. Plasmid-curing experiments revealed that the presence of plasmid pKB290-1 was essential for the strain's gastrointestinal tract tolerance and tendency to aggregate. Using next-generation deep sequencing of current and 18-year-old stock strains to detect low frequency variants, we evaluated genome stability. Deep sequencing of four periodic KB290 culture stocks with more than 1,000-fold coverage revealed 3 mutation sites and 37 minority variation sites, indicating long-term stability and providing a useful method for assessing the stability of industrial bacteria at the nucleotide level.
ESTHER : Fukao_2013_PLoS.One_8_e60521
PubMedSearch : Fukao_2013_PLoS.One_8_e60521
PubMedID: 23544154
Gene_locus related to this paper: lacba-q03sl1

Title : Draft Genome Sequence of Helicobacter fennelliae Strain MRY12-0050, Isolated from a Bacteremia Patient - Rimbara_2013_Genome.Announc_1_e00512
Author(s) : Rimbara E , Matsui M , Mori S , Suzuki S , Suzuki M , Kim H , Sekizuka T , Kuroda M , Shibayama K
Ref : Genome Announc , 1 : , 2013
Abstract : Helicobacter fennelliae, a human enterohepatic pathogen, causes bacteremia and colitis. We isolated H. fennelliae strain MRY12-0050 from a female patient; this strain was isolated from 2 other patients from the same hospital during the same period, suggesting human-to-human transmission. This is the first report of an H. fennelliae genome sequence.
ESTHER : Rimbara_2013_Genome.Announc_1_e00512
PubMedSearch : Rimbara_2013_Genome.Announc_1_e00512
PubMedID: 23929465

Title : Algal genomes reveal evolutionary mosaicism and the fate of nucleomorphs - Curtis_2012_Nature_492_59
Author(s) : Curtis BA , Tanifuji G , Burki F , Gruber A , Irimia M , Maruyama S , Arias MC , Ball SG , Gile GH , Hirakawa Y , Hopkins JF , Kuo A , Rensing SA , Schmutz J , Symeonidi A , Elias M , Eveleigh RJ , Herman EK , Klute MJ , Nakayama T , Obornik M , Reyes-Prieto A , Armbrust EV , Aves SJ , Beiko RG , Coutinho P , Dacks JB , Durnford DG , Fast NM , Green BR , Grisdale CJ , Hempel F , Henrissat B , Hoppner MP , Ishida K , Kim E , Koreny L , Kroth PG , Liu Y , Malik SB , Maier UG , McRose D , Mock T , Neilson JA , Onodera NT , Poole AM , Pritham EJ , Richards TA , Rocap G , Roy SW , Sarai C , Schaack S , Shirato S , Slamovits CH , Spencer DF , Suzuki S , Worden AZ , Zauner S , Barry K , Bell C , Bharti AK , Crow JA , Grimwood J , Kramer R , Lindquist E , Lucas S , Salamov A , McFadden GI , Lane CE , Keeling PJ , Gray MW , Grigoriev IV , Archibald JM
Ref : Nature , 492 :59 , 2012
Abstract : Cryptophyte and chlorarachniophyte algae are transitional forms in the widespread secondary endosymbiotic acquisition of photosynthesis by engulfment of eukaryotic algae. Unlike most secondary plastid-bearing algae, miniaturized versions of the endosymbiont nuclei (nucleomorphs) persist in cryptophytes and chlorarachniophytes. To determine why, and to address other fundamental questions about eukaryote-eukaryote endosymbiosis, we sequenced the nuclear genomes of the cryptophyte Guillardia theta and the chlorarachniophyte Bigelowiella natans. Both genomes have >21,000 protein genes and are intron rich, and B. natans exhibits unprecedented alternative splicing for a single-celled organism. Phylogenomic analyses and subcellular targeting predictions reveal extensive genetic and biochemical mosaicism, with both host- and endosymbiont-derived genes servicing the mitochondrion, the host cell cytosol, the plastid and the remnant endosymbiont cytosol of both algae. Mitochondrion-to-nucleus gene transfer still occurs in both organisms but plastid-to-nucleus and nucleomorph-to-nucleus transfers do not, which explains why a small residue of essential genes remains locked in each nucleomorph.
ESTHER : Curtis_2012_Nature_492_59
PubMedSearch : Curtis_2012_Nature_492_59
PubMedID: 23201678
Gene_locus related to this paper: guith-l1i9i5 , guith-l1k167 , guitc-l1jmn9

Title : Complete genome sequence of Mycoplasma pneumoniae type 2a strain 309, isolated in Japan - Kenri_2012_J.Bacteriol_194_1253
Author(s) : Kenri T , Horino A , Matsui M , Sasaki Y , Suzuki S , Narita M , Ohya H , Okazaki N , Shibayama K
Ref : Journal of Bacteriology , 194 :1253 , 2012
Abstract : Mycoplasma pneumoniae strain 309, a type 2a (subtype 2 variant) strain of this bacterium, has variations in the P1 protein, which is responsible for attachment of the bacterium to host cells. Here, we report the complete genome sequence of M. pneumoniae strain 309 isolated from a pneumonia patient in Japan.
ESTHER : Kenri_2012_J.Bacteriol_194_1253
PubMedSearch : Kenri_2012_J.Bacteriol_194_1253
PubMedID: 22328753
Gene_locus related to this paper: mycpn-pip

Title : Topical naphazoline in the treatment of myasthenic blepharoptosis - Nagane_2011_Muscle.Nerve_44_41
Author(s) : Nagane Y , Utsugisawa K , Suzuki S , Masuda M , Shimizu Y , Utsumi H , Uchiyama S , Suzuki N
Ref : Muscle & Nerve , 44 :41 , 2011
Abstract : INTRODUCTION: When treating ocular myasthenia gravis (MG), the risk/benefit profile of corticosteroids is unclear, and acetylcholinesterase inhibitors are not very effective. We examined the efficacy of topical naphazoline in the treatment of myasthenic blepharoptosis. METHODS: Sixty MG patients with blepharoptosis (32 with ocular symptoms only and 28 with mild generalized symptoms) were enrolled in a multicenter open trial of topical naphazoline. The effects were reported by patients via a questionnaire and were also confirmed for each patient at the clinic. RESULTS: Among 70 eyes of 60 patients, 20 eyes (28.6%) of 17 patients (28.3%) exhibited a marked response (full eye opening), and 24 eyes (34.3%) of 20 patients (33.3%) showed a good response (adequate but incomplete eye opening). Topical naphazoline was evaluated as useful in the treatment of myasthenic blepharoptosis by >70% of the patients. CONCLUSIONS: Topical naphazoline was found to be an effective supplementary symptomatic treatment for myasthenic blepharoptosis.
ESTHER : Nagane_2011_Muscle.Nerve_44_41
PubMedSearch : Nagane_2011_Muscle.Nerve_44_41
PubMedID: 21491460

Title : Comparison of sequence reads obtained from three next-generation sequencing platforms - Suzuki_2011_PLoS.One_6_e19534
Author(s) : Suzuki S , Ono N , Furusawa C , Ying BW , Yomo T
Ref : PLoS ONE , 6 :e19534 , 2011
Abstract : Next-generation sequencing technologies enable the rapid cost-effective production of sequence data. To evaluate the performance of these sequencing technologies, investigation of the quality of sequence reads obtained from these methods is important. In this study, we analyzed the quality of sequence reads and SNP detection performance using three commercially available next-generation sequencers, i.e., Roche Genome Sequencer FLX System (FLX), Illumina Genome Analyzer (GA), and Applied Biosystems SOLiD system (SOLiD). A common genomic DNA sample obtained from Escherichia coli strain DH1 was applied to these sequencers. The obtained sequence reads were aligned to the complete genome sequence of E. coli DH1, to evaluate the accuracy and sequence bias of these sequence methods. We found that the fraction of "junk" data, which could not be aligned to the reference genome, was largest in the data set of SOLiD, in which about half of reads could not be aligned. Among data sets after alignment to the reference, sequence accuracy was poorest in GA data sets, suggesting relatively low fidelity of the elongation reaction in the GA method. Furthermore, by aligning the sequence reads to the E. coli strain W3110, we screened sequence differences between two E. coli strains using data sets of three different next-generation platforms. The results revealed that the detected sequence differences were similar among these three methods, while the sequence coverage required for the detection was significantly small in the FLX data set. These results provided valuable information on the quality of short sequence reads and the performance of SNP detection in three next-generation sequencing platforms.
ESTHER : Suzuki_2011_PLoS.One_6_e19534
PubMedSearch : Suzuki_2011_PLoS.One_6_e19534
PubMedID: 21611185
Gene_locus related to this paper: ecoli-rutD

Title : Cadmium reduces adipocyte size and expression levels of adiponectin and Peg1\/Mest in adipose tissue - Kawakami_2010_Toxicology_267_20
Author(s) : Kawakami T , Sugimoto H , Furuichi R , Kadota Y , Inoue M , Setsu K , Suzuki S , Sato M
Ref : Toxicology , 267 :20 , 2010
Abstract : Adipose tissue dysfunction has been associated with diabetogenic effects. The effects of repeated Cd exposure on adipocytes remain largely unknown. We administered Cd at doses of 0, 5, 10, and 20 micromol/kgbw sc for 2 weeks (3.5 times/week) to mice and assessed the possible alteration of epididymal white adipose tissue (WAT), including histological difference, adipocyte differentiation and functional capacity. Whereas hepatic weight did not differ between the control and Cd-exposed groups, WAT weight, as well as adipose cell mass, significantly decreased in a dose-dependent manner in Cd-treated mice. The Cd concentration in WAT significantly increased in Cd-treated groups after 2 weeks of exposure. Next, we examined the effects of Cd on adipocyte differentiation and hypertrophy. Cd exposure significantly decreased the paternally expressed gene 1/Mesoderm-specific transcript mRNA expression levels. Both peroxisome proliferator-activated receptor gamma2 and CCAAT/enhancer-binding protein alpha mRNA expression levels in WAT tended to decrease in the Cd-treated groups. Next, we determined the effects of Cd exposure on the mRNA expression levels of adipose-derived hormones, such as adiponectin and resistin. The adiponectin mRNA expression level in WAT decreased after both 6h and 2 weeks of exposure to a high dose of Cd, and the reduction in resistin mRNA expression levels was observed after 2 weeks of exposure. These results suggest that Cd exposure causes abnormal adipocyte differentiation, expansion, and function, which might lead to development of insulin resistance, hypertension, and cardiovascular disease.
ESTHER : Kawakami_2010_Toxicology_267_20
PubMedSearch : Kawakami_2010_Toxicology_267_20
PubMedID: 19666079

Title : Characterization of recombinant prolyl aminopeptidase from Aspergillus oryzae - Matsushita-Morita_2010_J.Appl.Microbiol_109_156
Author(s) : Matsushita-Morita M , Furukawa I , Suzuki S , Yamagata Y , Koide Y , Ishida H , Takeuchi M , Kashiwagi Y , Kusumoto KI
Ref : J Appl Microbiol , 109 :156 , 2010
Abstract : AIMS: Prolyl aminopeptidase (PAP) degrades only amino-terminal proline from peptides. The food-grade fungus Aspergillus oryzae produces this enzyme only in small amounts. In this paper, we present efficient production of recombinant PAP with an overexpression system of A. oryzae and characterization of its biochemical properties. METHODS AND
RESULTS: The gene encoding PAP was overexpressed as a His-tag fusion protein under a taka-amylase gene (amyB) promoter with a limited expressing condition in A. oryzae. The PAP activity in the mycelia grown in rich medium containing glucose (repressing condition) was twice that in starch (inducing condition). The enzyme prepared as cell-free extract was partially purified through two-step column chromatography. The PAP was estimated to be a hexameric protein and exhibited salt tolerance against NaCl of up to 4 mol l(-1).
CONCLUSIONS: Aspergillus oryzae PAP was produced under the repressing condition of amyB promoter in a PAP-overexpressing strain and purified 1800-folds. Overproduction of PAP under promoter-inducing conditions led to an increase in inactive PAP, possibly because of irregular folding. SIGNIFICANCE AND IMPACT OF THE STUDY: PAP with a high specific activity and salt tolerance may be used effectively in the manufacturing processes of fermented foods.
ESTHER : Matsushita-Morita_2010_J.Appl.Microbiol_109_156
PubMedSearch : Matsushita-Morita_2010_J.Appl.Microbiol_109_156
PubMedID: 20028436
Gene_locus related to this paper: aspor-q2ulr2

Title : An outer membrane autotransporter, AoaA, of Azorhizobium caulinodans is required for sustaining high N2-fixing activity of stem nodules - Suzuki_2008_FEMS.Microbiol.Lett_285_16
Author(s) : Suzuki T , Aono T , Liu CT , Suzuki S , Iki T , Yokota K , Oyaizu H
Ref : FEMS Microbiology Letters , 285 :16 , 2008
Abstract : In this study, we investigated the function of a putative high-molecular-weight outer membrane protein, azorhizobial outer membrane autotransporter A (AoaA), of Azorhizobium caulinodans ORS571. Sequence analysis revealed that AoaA was an autotransporter protein belonging to the type V protein secretion system. Azorhizobium caulinodans forms N(2)-fixing nodules on the stems and roots of Sesbania rostrata. The sizes of stem nodules formed by an aoaA mutant having transposon insertion within this ORF were as large as those in the wild-type strain, but the N(2)-fixing activity of the nodules by the aoaA mutant was lower than that of wild-type nodules. cDNA-amplified fragment length polymorphism and reverse transcriptase-PCR analysis revealed that the expressions of several pathogen-related genes of host plants were induced in the aoaA mutant nodules. Furthermore, exopolysaccharide production was defective in the aoaA mutant under free-living conditions. These results indicate that AoaA may have an important role in sustaining the symbiosis by suppressing plant defense responses. The exopolysaccharide production controlled by AoaA might mediate this suppression mechanism.
ESTHER : Suzuki_2008_FEMS.Microbiol.Lett_285_16
PubMedSearch : Suzuki_2008_FEMS.Microbiol.Lett_285_16
PubMedID: 18557786

Title : Assessment of induction of cytochrome P450 by NO-1886 (ibrolipim), a lipoprotein lipase-promoting agent, in primary cultures of human hepatocytes and in female rat liver - Morioka_2006_Drug.Metab.Pharmacokinet_21_19
Author(s) : Morioka Y , Nishimura M , Imai T , Suzuki S , Harada M , Satoh T , Naito S
Ref : Drug Metab Pharmacokinet , 21 :19 , 2006
Abstract : The mRNA levels of human cytochrome P450 (CYP)2Cs and CYP3As in primary cultures of freshly isolated human hepatocytes were assessed after exposure to NO-1886 and rifampicin, a typical inducer of CYP3As. mRNA levels were analyzed by real-time RT-PCR using an ABI PRISM 7700 Sequence Detector system. Exposure to NO-1886 for 24 hr at a concentration of less than 10 microM showed only a tendency to reduce or increase the expression levels of CYP2C8, CYP2C9, CYP2C19, CYP3A4, or CYP3A5 mRNA. A higher concentration (50 microM) of NO-1886 induced an increase in CYP2C8 mRNA and a decrease in CYP2C19 mRNA, and these changes continued after additional culture for 24 hr in fresh medium without NO-1886. The expression level of CYP3A4 mRNA after exposure to NO-1886 for 24 hr at 50 microM was about twice that in controls. Following additional culture for 24 hr in fresh medium without NO-1886, the expression of CYP3A4 mRNA was comparable to that in controls. On the other hand, the expression levels of CYP2C9 and CYP3A5 mRNA showed small and variable changes in each donor even at a high concentration (50 microM) of NO-1886. Furthermore, the pharmacokinetics of NO-1886 during repeated oral administration for 14 days was studied in female rats. The pharmacokinetic parameters of NO-1886 were nearly the same on days 1, 7, and 14 of repeated administration. The hepatic microsomal content of CYP isoforms was not affected by repeated administration for 7 days at a dose of 1 to 30 mg/kg in female rats, although the total CYP content was increased at a dose of 30 mg/kg. The expression levels of CYP1A2, CYP2B2, CYP2C12, and CYP2E1 mRNA in primary cultures of rat hepatocytes were not affected by exposure to NO-1886 at 2, 10, or 50 microM. The expression levels of CYP3A1 mRNA in primary cultures of rat hepatocytes were not affected by exposure to NO-1886 at 2 or 10 microM, but were increased, with large individual variation, by exposure at 50 microM. The mRNA expression levels in rat hepatocytes exposed to concentrations comparable to free plasma levels did not change significantly, which was consistent with the equivalence in the in vivo plasma concentrations observed on days 1 and 14 of repeated administration. These results suggest that repeated administration of NO-1886 at clinical doses does not significantly affect the expression levels of CYP isoforms in human liver, although the mRNA levels of the CYP isoforms involved in the metabolism of NO-1886 were increased by exposure to higher concentrations of NO-1886 in human hepatocytes in vitro.
ESTHER : Morioka_2006_Drug.Metab.Pharmacokinet_21_19
PubMedSearch : Morioka_2006_Drug.Metab.Pharmacokinet_21_19
PubMedID: 16547390

Title : New safe method for preparation of sarin-exposed human erythrocytes acetylcholinesterase using non-toxic and stable sarin analogue isopropyl p-nitrophenyl methylphosphonate and its application to evaluation of nerve agent antidotes - Ohta_2006_Pharm.Res_23_2827
Author(s) : Ohta H , Ohmori T , Suzuki S , Ikegaya H , Sakurada K , Takatori T
Ref : Pharm Res , 23 :2827 , 2006
Abstract : INTRODUCTION: A non-toxic and stable sarin analogue, isopropyl p-nitrophenyl methylphosphonate (INMP), was synthesized for safe preparation of sarin-exposed acetylcholinesterase (AChE). RESULTS AND DISCUSSION: This agent was stable for years, able to be handled in an ordinary laboratory without special care, and its 50% inhibitory concentration (IC50) on 0.04 U/ml human erythrocytes AChE was 15 nM. This reagent was thought to be especially useful since it enables experiments that require sarin-inhibited AChE, such as the development of antidotes for sarin, in a usual laboratory. To demonstrate the usefulness of this method, 40 known and novel pyridinealdoxime methiodide (PAM)-type oxime antidotes were synthesized, and their reactivation activities to INMP-exposed AChE and structure-activities correlation were studied. CONCLUSION: Among the antidotes tested in this experiment except for 2-PAM, the compound found to have the highest reactivation activity, was the novel hydrophobic 2-PAM-type compound, 2-[(hydroxyimino)methyl]-1-[4-(tert-butyl)benzyl] pyridinium bromide.
ESTHER : Ohta_2006_Pharm.Res_23_2827
PubMedSearch : Ohta_2006_Pharm.Res_23_2827
PubMedID: 17096183

Title : The transcriptional landscape of the mammalian genome - Carninci_2005_Science_309_1559
Author(s) : Carninci P , Kasukawa T , Katayama S , Gough J , Frith MC , Maeda N , Oyama R , Ravasi T , Lenhard B , Wells C , Kodzius R , Shimokawa K , Bajic VB , Brenner SE , Batalov S , Forrest AR , Zavolan M , Davis MJ , Wilming LG , Aidinis V , Allen JE , Ambesi-Impiombato A , Apweiler R , Aturaliya RN , Bailey TL , Bansal M , Baxter L , Beisel KW , Bersano T , Bono H , Chalk AM , Chiu KP , Choudhary V , Christoffels A , Clutterbuck DR , Crowe ML , Dalla E , Dalrymple BP , de Bono B , Della Gatta G , di Bernardo D , Down T , Engstrom P , Fagiolini M , Faulkner G , Fletcher CF , Fukushima T , Furuno M , Futaki S , Gariboldi M , Georgii-Hemming P , Gingeras TR , Gojobori T , Green RE , Gustincich S , Harbers M , Hayashi Y , Hensch TK , Hirokawa N , Hill D , Huminiecki L , Iacono M , Ikeo K , Iwama A , Ishikawa T , Jakt M , Kanapin A , Katoh M , Kawasawa Y , Kelso J , Kitamura H , Kitano H , Kollias G , Krishnan SP , Kruger A , Kummerfeld SK , Kurochkin IV , Lareau LF , Lazarevic D , Lipovich L , Liu J , Liuni S , McWilliam S , Madan Babu M , Madera M , Marchionni L , Matsuda H , Matsuzawa S , Miki H , Mignone F , Miyake S , Morris K , Mottagui-Tabar S , Mulder N , Nakano N , Nakauchi H , Ng P , Nilsson R , Nishiguchi S , Nishikawa S , Nori F , Ohara O , Okazaki Y , Orlando V , Pang KC , Pavan WJ , Pavesi G , Pesole G , Petrovsky N , Piazza S , Reed J , Reid JF , Ring BZ , Ringwald M , Rost B , Ruan Y , Salzberg SL , Sandelin A , Schneider C , Schonbach C , Sekiguchi K , Semple CA , Seno S , Sessa L , Sheng Y , Shibata Y , Shimada H , Shimada K , Silva D , Sinclair B , Sperling S , Stupka E , Sugiura K , Sultana R , Takenaka Y , Taki K , Tammoja K , Tan SL , Tang S , Taylor MS , Tegner J , Teichmann SA , Ueda HR , van Nimwegen E , Verardo R , Wei CL , Yagi K , Yamanishi H , Zabarovsky E , Zhu S , Zimmer A , Hide W , Bult C , Grimmond SM , Teasdale RD , Liu ET , Brusic V , Quackenbush J , Wahlestedt C , Mattick JS , Hume DA , Kai C , Sasaki D , Tomaru Y , Fukuda S , Kanamori-Katayama M , Suzuki M , Aoki J , Arakawa T , Iida J , Imamura K , Itoh M , Kato T , Kawaji H , Kawagashira N , Kawashima T , Kojima M , Kondo S , Konno H , Nakano K , Ninomiya N , Nishio T , Okada M , Plessy C , Shibata K , Shiraki T , Suzuki S , Tagami M , Waki K , Watahiki A , Okamura-Oho Y , Suzuki H , Kawai J , Hayashizaki Y
Ref : Science , 309 :1559 , 2005
Abstract : This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development.
ESTHER : Carninci_2005_Science_309_1559
PubMedSearch : Carninci_2005_Science_309_1559
PubMedID: 16141072
Gene_locus related to this paper: mouse-abhd1 , mouse-abhd3 , mouse-abhd4 , mouse-acot4 , mouse-adcl4 , mouse-DGLB , mouse-ephx3 , mouse-Kansl3 , mouse-lipli , mouse-LIPN , mouse-Ppgb , mouse-q3uuq7 , mouse-srac1 , mouse-Tex30 , mouse-tmco4 , mouse-tmm53 , mouse-f172a

Title : Genomic imprinting of IGF2, p57(KIP2) and PEG1\/MEST in a marsupial, the tammar wallaby - Suzuki_2005_Mech.Dev_122_213
Author(s) : Suzuki S , Renfree MB , Pask AJ , Shaw G , Kobayashi S , Kohda T , Kaneko-Ishino T , Ishino F
Ref : Mech Dev , 122 :213 , 2005
Abstract : Genomic imprinting is widespread amongst mammals, but has not yet been found in birds. To gain a broader understanding of the origin and significance of imprinting, we have characterized three genes, from three separate imprinted clusters in eutherian mammals in the developing fetus and placenta of an Australian marsupial, the tammar wallaby Macropus eugenii. Imprinted gene orthologues of human and mouse p57(KIP2), IGF2 and PEG1/MEST genes were isolated. p57(KIP2) did not show stable monoallelic expression suggesting that it is not imprinted in marsupials. In contrast, there was paternal-specific expression of IGF2 in almost all tissues, but the biased paternal expression of IGF2 in the fetal head and placenta, demonstrates the occurrence of tissue-specific imprinting, as occurs in mice and humans. There was also paternal-biased expression of PEG1/MESTalpha. The differentially methylated region (DMR) of the human and mouse PEG1/MEST promoter is absent in the wallaby. These data confirm the existence of common imprinted regions in eutherians and marsupials during development, but suggest that the regulatory mechanisms that control imprinted gene expression differ between these two groups of mammals.
ESTHER : Suzuki_2005_Mech.Dev_122_213
PubMedSearch : Suzuki_2005_Mech.Dev_122_213
PubMedID: 15652708

Title : Extremely sensitive biomarker of acute organophosphorus insecticide exposure - Fujikawa_2005_Hum.Exp.Toxicol_24_333
Author(s) : Fujikawa Y , Satoh T , Suganuma A , Suzuki S , Niikura Y , Yui S , Yamaura Y
Ref : Hum Exp Toxicol , 24 :333 , 2005
Abstract : Egasyn-beta-glucuronidase complex is located at the luminal site of liver microsomal endoplasmic reticulum. When organophosphorus insecticides (OP) are incorporated into the liver microsomes, they become tightly bound to egasyn, a carboxylesterase isozyme, and subsequently, beta-glucuronidase (BG) is dissociated and released into blood. Consequently, the increase in plasma BG activity becomes a good biomarker of OP exposure. Thus, the single administration of EPN (O-ethyl O-p-nitrophenylphenylphosphonothioate), acephate and chlorpyrifos increased plasma BG activity in approximately 100-fold the control level in rats. The increase in plasma BG activity after OP exposure is a much more sensitive biomarker of acute OP exposure than acetylcholinesterase (AChE) inhibition.
ESTHER : Fujikawa_2005_Hum.Exp.Toxicol_24_333
PubMedSearch : Fujikawa_2005_Hum.Exp.Toxicol_24_333
PubMedID: 16004201
Gene_locus related to this paper: human-CES1 , mouse-Ces1e , ratno-Ces1e

Title : Effect of Long-Term Successive Applications of Organic Fertilizers on Dissipation of Several Pesticides in Two Soils - Suzuki_2004_J.Pestic.Sci_29_33
Author(s) : Suzuki S , Otani T
Ref : Journal of Pesticide Science , 29 :33 , 2004
Abstract : The dissipation rates of dimethoate, fenobucarb, flutolanil, simazine, prometryn and alachlor were compared among an andosol and a gray lowland soil subjected to different fertilizing practices over a 20 year period. The rate constants for dissipation of most pesticides per biomass carbon, biomass nitrogen and esterase activity among the plots in each soil were less variable than the corresponding rate constants, indicating that the dissipation depended on microbial amount and activity. The rate constants in the gray lowland soil were similar to or greater than those in the andosol, despite the smaller values of microbial amount and activity in the former. This is due to the larger water soluble fraction/acetone soluble fraction ratios in the former. The long-term successive applications of organic fertilizers were less effective in the dissipation for the gray lowland soil than the andosol. This is likely to result from a less effective accumulation of microbial biomass in the former.
ESTHER : Suzuki_2004_J.Pestic.Sci_29_33
PubMedSearch : Suzuki_2004_J.Pestic.Sci_29_33

Title : Toxicological significance in the cleavage of esterase-beta-glucuronidase complex in liver microsomes by organophosphorus compounds - Satoh_1999_Chem.Biol.Interact_119-120_471
Author(s) : Satoh T , Suzuki S , Kawai N , Nakamura T , Hosokawa M
Ref : Chemico-Biological Interactions , 119-120 :471 , 1999
Abstract : Egasyn is an accessory protein of beta-glucuronidase (beta-G) in the liver microsomes. Liver microsomal beta-G is stabilized within the luminal site of the microsomal vesicles by complexation with egasyn which is one of the carboxylesterase isozymes. We investigated the effects of organophosphorus compounds (OPs) such as insecticides on the dissociation of egasyn-beta-glucuronidase (EG) complex. The EG complex was easily dissociated by administration of OPs, i.e. fenitrothion, EPN, phenthionate, and bis-beta-nitrophenyl phosphate (BNPP), and resulting beta-G dissociated was released into blood, leading to the rapid and transient increase of plasma beta-G level with a concomitant decrease of liver microsomal beta-G level. In a case of phenthionate treatment, less increase in plasma beta-G level was observed, as compared with those of other OPs. This may be explained by the fact that phenthionate was easily hydrolyzed by carboxylesterase. Similarly, carbamate insecticides such as carbaryl caused rapid increase of plasma beta-G level. In contrast, no significant increase of plasma beta-G level was observed when pyrethroid insecticides were administered to rats. This is due to the fact that pyrethroids such as phenthrin and allethrin were easily hydrolyzed by A-esterase as well as carboxylesterase. On the other hand, addition of OPs to the incubation mixture containing liver microsomes caused the release of beta-G from microsomes to the medium. From these in vivo and in vitro data, it is concluded that increase of the plasma beta-G level after OP administration is much more sensitive biomarker than cholinesterase inhibition to acute intoxication of OPs and carbamates.
ESTHER : Satoh_1999_Chem.Biol.Interact_119-120_471
PubMedSearch : Satoh_1999_Chem.Biol.Interact_119-120_471
PubMedID: 10421485
Gene_locus related to this paper: mouse-Ces1e

Title : [Comparative studies on multiple forms of serum cholinesterase in various species] - Unakami_1987_Jikken.Dobutsu_36_199
Author(s) : Unakami S , Suzuki S , Nakanishi E , Ichinohe K , Hirata M , Tanimoto Y
Ref : Jikken Dobutsu , 36 :199 , 1987
Abstract : Multiple forms of serum cholinesterase (ChE) were compared in 8 species by electrophoretic technique and the following characteristics were noted. The first moving fraction markedly hydrolyzed butyrylthiocholine and the activity was not inhibited by 10(-5)M eserine in the serum of some rabbits tested. Electrophoretic patterns of the ChE were obtained by use of two thiocholines as substrate, and the number of fractions against acetylthiocholine were more than against butyrylthiocholine in dogs, miniature pigs, rabbits, and hamsters. The activities of ChE fractions of dogs (C3), miniature pigs (C1, C2), rabbits (C1), and hamsters (C3) were inhibited by 6.1 X 10(-2)M caffein but not by 10(-4)M ethopropazine, which suggests that the fractions are all true-ChE.
ESTHER : Unakami_1987_Jikken.Dobutsu_36_199
PubMedSearch : Unakami_1987_Jikken.Dobutsu_36_199
PubMedID: 3609156