Kim E

References (29)

Title : Association of cognitive enhancers and incident seizure risk in dementia: a population-based study - Ha_2022_BMC.Geriatr_22_480
Author(s) : Ha J , Son NH , Park YH , Lee E , Kim E , Jung Kim W
Ref : BMC Geriatr , 22 :480 , 2022
Abstract : BACKGROUND: Although individuals with dementia have a high risk of developing seizures, whether seizures are associated with cholinesterase inhibitors, which are commonly prescribed to treat individuals with dementia, remains unknown. This study investigated the risk of incident seizure following cholinesterase inhibitor use in patients with dementia. METHODS: A nationwide, nested case-control study was conducted using data from the Korean Health Insurance Review and Assessment Service (HIRA) from 2014 through 2018. A total of 13,767 participants aged 65-95 years who experienced incident seizure were propensity score-matched for medical comorbidities and drug exposure at a 1:3 ratio with a control group of 39,084 participants. The study examined the incidence of seizures in patients diagnosed with dementia within one year after receiving cognitive enhancers. Adjusted odds ratios (aORs) and 95% confidence intervals (CIs) for seizure incidence according to cholinesterase inhibitor use were analyzed using a multivariable conditional logistic regression model. RESULTS: There was no statistically significant association between duration of cholinesterase inhibitors use and seizure risk. Although there was slight increased seizure risk in patient after receiving donepezil for 1 year compared to memantine, subgroup analyses stratified age and sex did not reveal any significant association between cholinesterase inhibitors use and late-onset seizure. CONCLUSIONS: Our findings suggest no immediate increase in seizure risk is associated with cholinesterase inhibitor use, although the risk of seizure in patients with dementia did increase after one year of continued medication intake. Further study is required to obtain confirmatory results on the seizure-related safety of cognitive enhancers in patients with dementia.
ESTHER : Ha_2022_BMC.Geriatr_22_480
PubMedSearch : Ha_2022_BMC.Geriatr_22_480
PubMedID: 35658833

Title : Dataset of Swimming behavioral alterations in Danio rerio by Nemopilema nomurai jellyfish venom - Prakash_2021_Data.Brief_34_106721
Author(s) : Prakash RLM , Hwang DH , Hong IH , Chae J , Kang C , Kim E
Ref : Data Brief , 34 :106721 , 2021
Abstract : This article reports data associated with Prakash et al. [1]. Nemopilema nomurai jellyfish venom (NnV) can lead to neurotoxicity in zebrafish (Danio rerio) model. In the present study, zebrafish were treated with NnV by intraperitoneal injection and the swimming behavior of each fish was evaluated using a score scale. The dose of NnV in each treatment group was based on the protein concentration of NnV. Swimming is the main locomotory movements in the fishes. NnV modulated the swimming behavior of Danio rerio in a dose-dependent manner. In this article provided data are directly related to the previously published research article - "Danio rerio as an alternative vertebrate model for jellyfish venom study: the toxinological aspects of Nemopilema nomurai venom" [1] where the downregulation of acetylcholinesterase activity as well as histopathological alterations were observed from the brain of Danio rerio treated with NnV. Here we provide datasets, including mortality rate table, swimming behavior graph, and videos of zebrafish after NnV envenomation.
ESTHER : Prakash_2021_Data.Brief_34_106721
PubMedSearch : Prakash_2021_Data.Brief_34_106721
PubMedID: 33537367

Title : Danio rerio as an alternative vertebrate model for jellyfish venom study: The toxinological aspects of Nemopilema nomurai venom - Mohan_2020_Toxicol.Lett_335_91
Author(s) : Mohan Prakash RL , Hwang DH , Hong IH , Chae J , Kang C , Kim E
Ref : Toxicol Lett , 335 :91 , 2020
Abstract : Nemopilema nomurai venom (NnV) is severely toxic to many organisms. However, the mechanism of its poisoning has not been properly understood yet. The present work demonstrates that zebrafish (Danio rerio) is an alternative vertebrate model for studying NnV jellyfish venom for the first time. In this model, NnV appears to cause severe hemorrhage and inflammation in cardiopulmonary regions of zebrafish. NnV also altered the swimming behavior of zebrafish accompanied by a significant downregulation of acetylcholinesterase (AChE) activity in brain tissues. Histopathological changes observed for various organs of D. rerio caused by NnV corresponded to an increase in lactate dehydrogenase (LDH) activity in tissues. NnV also significantly altered glutathione S-transferase (GST) activity in cardiopulmonary and brain tissues of D. rerio. SDS-PAGE revealed many protein bands of NnV of various sizes after silver staining. Taken together, these results indicate that Danio rerio can be a useful alternative animal model for jellyfish venom toxicology studies. Findings of the present study also suggest that Danio rerio could be used to develop an effective treatment strategy and discover the mechanism of action of jellyfish venom envenomation.
ESTHER : Mohan_2020_Toxicol.Lett_335_91
PubMedSearch : Mohan_2020_Toxicol.Lett_335_91
PubMedID: 33157172

Title : Neuromuscular blockade reversal with sugammadex versus pyridostigmine\/glycopyrrolate in laparoscopic cholecystectomy: a randomized trial of effects on postoperative gastrointestinal motility - An_2020_Korean.J.Anesthesiol_73_137
Author(s) : An J , Noh H , Kim E , Lee J , Woo K , Kim H
Ref : Korean J Anesthesiol , 73 :137 , 2020
Abstract : BACKGROUND: Acetylcholinesterase inhibitors (e.g., pyridostigmine bromide) are used for neuromuscular blockade (NMB) reversal in patients undergoing surgery under general anesthesia (GA). Concurrent use of anticholinergic agents (e.g., glycopyrrolate) decreases cholinergic side effects but can impede bowel movements. Sugammadex has no cholinergic effects; its use modifies recovery of gastrointestinal (GI) motility following laparoscopic cholecystectomy compared to pyridostigmine/glycopyrrolate. This study evaluated the contribution of sugammadex to the recovery of GI motility compared with pyridostigmine and glycopyrrolate. METHODS: We conducted a prospective study of patients who underwent laparoscopic cholecystectomy. Patients were randomly allocated to the experimental group (sugammadex, Group S) or control group (pyridostigmine-glycopyrrolate, Group P). After anesthesia (propofol and rocuronium, and 2% sevoflurane), recovery was induced by injection of sugammadex or a pyridostigmine-glycopyrrolate mixture. As a primary outcome, patients recorded the time of their first passage of flatus ('gas-out time') and defecation. The secondary outcome was stool types. RESULTS: One-hundred and two patients participated (Group S, 49; Group P, 53). Mean time from injection of NMB reversal agents to gas-out time was 15.03 (6.36-20.25) h in Group S and 20.85 (16.34-25.86) h in Group P (P = 0.001). Inter-group differences were significant. Time until the first defecation as well as types of stools was not significantly different. CONCLUSIONS: Sugammadex after laparoscopic cholecystectomy under GA resulted in an earlier first postoperative passage of flatus compared with the use of a mixture of pyridostigmine and glycopyrrolate. These findings suggest that the use of sugammadex has positive effects on the recovery of GI motility.
ESTHER : An_2020_Korean.J.Anesthesiol_73_137
PubMedSearch : An_2020_Korean.J.Anesthesiol_73_137
PubMedID: 31636242

Title : Protein-altering variants associated with body mass index implicate pathways that control energy intake and expenditure in obesity - Turcot_2018_Nat.Genet_50_26
Author(s) : Turcot V , Lu Y , Highland HM , Schurmann C , Justice AE , Fine RS , Bradfield JP , Esko T , Giri A , Graff M , Guo X , Hendricks AE , Karaderi T , Lempradl A , Locke AE , Mahajan A , Marouli E , Sivapalaratnam S , Young KL , Alfred T , Feitosa MF , Masca NGD , Manning AK , Medina-Gomez C , Mudgal P , Ng MCY , Reiner AP , Vedantam S , Willems SM , Winkler TW , Abecasis G , Aben KK , Alam DS , Alharthi SE , Allison M , Amouyel P , Asselbergs FW , Auer PL , Balkau B , Bang LE , Barroso I , Bastarache L , Benn M , Bergmann S , Bielak LF , Bluher M , Boehnke M , Boeing H , Boerwinkle E , Boger CA , Bork-Jensen J , Bots ML , Bottinger EP , Bowden DW , Brandslund I , Breen G , Brilliant MH , Broer L , Brumat M , Burt AA , Butterworth AS , Campbell PT , Cappellani S , Carey DJ , Catamo E , Caulfield MJ , Chambers JC , Chasman DI , Chen YI , Chowdhury R , Christensen C , Chu AY , Cocca M , Collins FS , Cook JP , Corley J , Corominas Galbany J , Cox AJ , Crosslin DS , Cuellar-Partida G , D'Eustacchio A , Danesh J , Davies G , Bakker PIW , Groot MCH , Mutsert R , Deary IJ , Dedoussis G , Demerath EW , Heijer M , Hollander AI , Ruijter HM , Dennis JG , Denny JC , Angelantonio E , Drenos F , Du M , Dube MP , Dunning AM , Easton DF , Edwards TL , Ellinghaus D , Ellinor PT , Elliott P , Evangelou E , Farmaki AE , Farooqi IS , Faul JD , Fauser S , Feng S , Ferrannini E , Ferrieres J , Florez JC , Ford I , Fornage M , Franco OH , Franke A , Franks PW , Friedrich N , Frikke-Schmidt R , Galesloot TE , Gan W , Gandin I , Gasparini P , Gibson J , Giedraitis V , Gjesing AP , Gordon-Larsen P , Gorski M , Grabe HJ , Grant SFA , Grarup N , Griffiths HL , Grove ML , Gudnason V , Gustafsson S , Haessler J , Hakonarson H , Hammerschlag AR , Hansen T , Harris KM , Harris TB , Hattersley AT , Have CT , Hayward C , He L , Heard-Costa NL , Heath AC , Heid IM , Helgeland O , Hernesniemi J , Hewitt AW , Holmen OL , Hovingh GK , Howson JMM , Hu Y , Huang PL , Huffman JE , Ikram MA , Ingelsson E , Jackson AU , Jansson JH , Jarvik GP , Jensen GB , Jia Y , Johansson S , Jorgensen ME , Jorgensen T , Jukema JW , Kahali B , Kahn RS , Kahonen M , Kamstrup PR , Kanoni S , Kaprio J , Karaleftheri M , Kardia SLR , Karpe F , Kathiresan S , Kee F , Kiemeney LA , Kim E , Kitajima H , Komulainen P , Kooner JS , Kooperberg C , Korhonen T , Kovacs P , Kuivaniemi H , Kutalik Z , Kuulasmaa K , Kuusisto J , Laakso M , Lakka TA , Lamparter D , Lange EM , Lange LA , Langenberg C , Larson EB , Lee NR , Lehtimaki T , Lewis CE , Li H , Li J , Li-Gao R , Lin H , Lin KH , Lin LA , Lin X , Lind L , Lindstrom J , Linneberg A , Liu CT , Liu DJ , Liu Y , Lo KS , Lophatananon A , Lotery AJ , Loukola A , Luan J , Lubitz SA , Lyytikainen LP , Mannisto S , Marenne G , Mazul AL , McCarthy MI , McKean-Cowdin R , Medland SE , Meidtner K , Milani L , Mistry V , Mitchell P , Mohlke KL , Moilanen L , Moitry M , Montgomery GW , Mook-Kanamori DO , Moore C , Mori TA , Morris AD , Morris AP , Muller-Nurasyid M , Munroe PB , Nalls MA , Narisu N , Nelson CP , Neville M , Nielsen SF , Nikus K , Njolstad PR , Nordestgaard BG , Nyholt DR , O'Connel JR , O'Donoghue ML , Olde Loohuis LM , Ophoff RA , Owen KR , Packard CJ , Padmanabhan S , Palmer CNA , Palmer ND , Pasterkamp G , Patel AP , Pattie A , Pedersen O , Peissig PL , Peloso GM , Pennell CE , Perola M , Perry JA , Perry JRB , Pers TH , Person TN , Peters A , Petersen ERB , Peyser PA , Pirie A , Polasek O , Polderman TJ , Puolijoki H , Raitakari OT , Rasheed A , Rauramaa R , Reilly DF , Renstrom F , Rheinberger M , Ridker PM , Rioux JD , Rivas MA , Roberts DJ , Robertson NR , Robino A , Rolandsson O , Rudan I , Ruth KS , Saleheen D , Salomaa V , Samani NJ , Sapkota Y , Sattar N , Schoen RE , Schreiner PJ , Schulze MB , Scott RA , Segura-Lepe MP , Shah SH , Sheu WH , Sim X , Slater AJ , Small KS , Smith AV , Southam L , Spector TD , Speliotes EK , Starr JM , Stefansson K , Steinthorsdottir V , Stirrups KE , Strauch K , Stringham HM , Stumvoll M , Sun L , Surendran P , Swift AJ , Tada H , Tansey KE , Tardif JC , Taylor KD , Teumer A , Thompson DJ , Thorleifsson G , Thorsteinsdottir U , Thuesen BH , Tonjes A , Tromp G , Trompet S , Tsafantakis E , Tuomilehto J , Tybjaerg-Hansen A , Tyrer JP , Uher R , Uitterlinden AG , Uusitupa M , Laan SW , Duijn CM , Leeuwen N , van Setten J , Vanhala M , Varbo A , Varga TV , Varma R , Velez Edwards DR , Vermeulen SH , Veronesi G , Vestergaard H , Vitart V , Vogt TF , Volker U , Vuckovic D , Wagenknecht LE , Walker M , Wallentin L , Wang F , Wang CA , Wang S , Wang Y , Ware EB , Wareham NJ , Warren HR , Waterworth DM , Wessel J , White HD , Willer CJ , Wilson JG , Witte DR , Wood AR , Wu Y , Yaghootkar H , Yao J , Yao P , Yerges-Armstrong LM , Young R , Zeggini E , Zhan X , Zhang W , Zhao JH , Zhao W , Zhou W , Zondervan KT , Rotter JI , Pospisilik JA , Rivadeneira F , Borecki IB , Deloukas P , Frayling TM , Lettre G , North KE , Lindgren CM , Hirschhorn JN , Loos RJF
Ref : Nat Genet , 50 :26 , 2018
Abstract : Genome-wide association studies (GWAS) have identified >250 loci for body mass index (BMI), implicating pathways related to neuronal biology. Most GWAS loci represent clusters of common, noncoding variants from which pinpointing causal genes remains challenging. Here we combined data from 718,734 individuals to discover rare and low-frequency (minor allele frequency (MAF) < 5%) coding variants associated with BMI. We identified 14 coding variants in 13 genes, of which 8 variants were in genes (ZBTB7B, ACHE, RAPGEF3, RAB21, ZFHX3, ENTPD6, ZFR2 and ZNF169) newly implicated in human obesity, 2 variants were in genes (MC4R and KSR2) previously observed to be mutated in extreme obesity and 2 variants were in GIPR. The effect sizes of rare variants are ~10 times larger than those of common variants, with the largest effect observed in carriers of an MC4R mutation introducing a stop codon (p.Tyr35Ter, MAF = 0.01%), who weighed ~7 kg more than non-carriers. Pathway analyses based on the variants associated with BMI confirm enrichment of neuronal genes and provide new evidence for adipocyte and energy expenditure biology, widening the potential of genetically supported therapeutic targets in obesity.
ESTHER : Turcot_2018_Nat.Genet_50_26
PubMedSearch : Turcot_2018_Nat.Genet_50_26
PubMedID: 29273807

Title : Structural Insights into Modulation of Neurexin-Neuroligin Trans-synaptic Adhesion by MDGA1\/Neuroligin-2 Complex - Kim_2017_Neuron_94_1121
Author(s) : Kim JA , Kim D , Won SY , Han KA , Park D , Cho E , Yun N , An HJ , Um JW , Kim E , Lee JO , Ko J , Kim HM
Ref : Neuron , 94 :1121 , 2017
Abstract : Membrane-associated mucin domain-containing glycosylphosphatidylinositol anchor proteins (MDGAs) bind directly to neuroligin-1 (NL1) and neuroligin-2 (NL2), thereby respectively regulating excitatory and inhibitory synapse development. However, the mechanisms by which MDGAs modulate NL activity to specify development of the two synapse types remain unclear. Here, we determined the crystal structures of human NL2/MDGA1 Ig1-3 complex, revealing their stable 2:2 arrangement with three interaction interfaces. Cell-based assays using structure-guided, site-directed MDGA1 mutants showed that all three contact patches were required for the MDGA's negative regulation of NL2-mediated synaptogenic activity. Furthermore, MDGA1 competed with neurexins for NL2 via its Ig1 domain. The binding affinities of both MDGA1 and MDGA2 for NL1 and NL2 were similar, consistent with the structural prediction of similar binding interfaces. However, MDGA1 selectively associated with NL2, but not NL1, in vivo. These findings collectively provide structural insights into the mechanism by which MDGAs negatively modulate synapse development governed by NLs/neurexins.
ESTHER : Kim_2017_Neuron_94_1121
PubMedSearch : Kim_2017_Neuron_94_1121
PubMedID: 28641111
Gene_locus related to this paper: human-NLGN2

Title : Cholinesterase Inhibitor Donepezil Increases Mitochondrial Biogenesis through AMP-Activated Protein Kinase in the Hippocampus - Kim_2016_Neuropsychobiology_73_81
Author(s) : Kim E , Park M , Jeong J , Kim H , Lee SK , Lee E , Oh BH , Namkoong K
Ref : Neuropsychobiology , 73 :81 , 2016
Abstract : OBJECTIVE: Donepezil, a widely prescribed drug for Alzheimer's disease (AD), is now considered to have multimodal actions beyond cholinesterase inhibition. We aimed to see whether donepezil enhances mitochondrial biogenesis and relevant signaling pathways since mitochondrial dysfunction is a key feature of the hypometabolic AD brain.
METHODS: As a metabolic gauge, AMP-activated protein kinase (AMPK) was investigated as a tentative mediator of neurometabolic action of donepezil. Changes in phospho-AMPK levels, mitochondrial biogenesis, and ATP levels were measured upon donepezil treatment using neuroblastoma cells, primary cultured neurons and ex vivo hippocampal tissue of adult mice.
RESULTS: Donepezil dose-dependently increased mitochondrial biogenesis and ATP levels as well as expression of PGC-1alpha and NRF-1 in neuroblastoma cells. Donepezil dose-dependently activated AMPK; however, inhibition of AMPK abolished the observed effects of donepezil, indicating that AMPK is a key mediator of donepezil's action. Notably, mitochondrial biogenesis upon donepezil treatment was mainly observed within dendritic regions of primary cultured hippocampal neurons. Levels of synaptic markers were also increased by donepezil. Finally, AMPK- dependent mitochondrial biogenesis by donepezil was confirmed in organotypic hippocampal tissue.
CONCLUSIONS: Our findings indicate that AMPK/PGC-1alpha signaling is involved in beneficial actions of donepezil on neurometabolism. Pharmacological activation of AMPK might be a promising approach to counteract AD pathogenesis associated with brain hypometabolism.
ESTHER : Kim_2016_Neuropsychobiology_73_81
PubMedSearch : Kim_2016_Neuropsychobiology_73_81
PubMedID: 27002982

Title : In vitro metabolism of flucetosulfuron by human liver microsomes - Lee_2014_J.Agric.Food.Chem_62_3057
Author(s) : Lee YS , Liu KH , Moon JK , Ko BJ , Choi H , Hwang KS , Kim E , Kim JH
Ref : Journal of Agricultural and Food Chemistry , 62 :3057 , 2014
Abstract : To investigate herbicide metabolism, human liver microsomes were incubated with threo- and erythro-isomers of flucetosulfuron. Each isomer produced one metabolite; the metabolites were unambiguously identified as enzymatic hydrolysis products by using liquid chromatography-mass spectrometry (LC-MS). These metabolites were synthesized, producing white solids characterized using LC-MS and nuclear magnetic resonance spectroscopy (1H and 13C). Using specific esterase inhibitors and activators, carboxylesterases and cholinesterases were demonstrated to be involved in flucetosulfuron metabolism. Under optimized metabolic conditions, the kinetic parameters for metabolite formation from erythro-flucetosulfuron and threo-flucetosulfuron were: Vmax, 134.38 and 151.41 nmol/min/mg protein, respectively; Km, 2798.53 and 2957.37 muM, respectively; and CLint, 48.02 and 51.20 muL/min/mg microsomes, respectively. No significant kinetic differences were observed between the two isomers. These results indicated that the primary metabolic pathway for both flucetosulfuron isomers in human liver microsomes involves hydrolysis, catalyzed by carboxylesterase and cholinesterase.
ESTHER : Lee_2014_J.Agric.Food.Chem_62_3057
PubMedSearch : Lee_2014_J.Agric.Food.Chem_62_3057
PubMedID: 24628475

Title : Inhibition of acetylcholinesterases of the pinewood nematode, Bursaphelenchus xylophilus, by phytochemicals from plant essential oils - Kang_2013_Pestic.Biochem.Physiol_105_50
Author(s) : Kang JS , Kim E , Lee SH , Park IK
Ref : Pesticide Biochemistry and Physiology , 105 :50 , 2013
Abstract : To understand the nematicidal mode of action of phytochemicals derived from plant essential oils against the pinewood nematode (Bursaphelenchus xylophilus), we evaluated 97 compounds (49 monoterpenes, 17 phenylpropenes, 16 sesquiterpenes, and 15 sulfides) for their inhibitory effects on B. xylophilus acetylcholinesterases (BxACEs). In the primary inhibition assay using B. xylophilus crude protein, more than 50% BxACE inhibition activity was observed with 3 monoterpenes, (+)-alpha-pinene, (-)-alpha-pinene, and 3-carene; 2 phenylpropenes, omicron-anisaldehyde, and coniferyl alcohol; and 1 sesquiterpene, cis-nerolidol. Other compounds showed moderate or weak inhibitory activity. The inhibitory activities against 3 recombinant BxACEs were subsequently estimated using the identified active compounds in a primary inhibition assay. (+)-alpha-Pinene showed the strongest inhibition of BxACE-1 followed by 3-carene, coniferyl alcohol, (-)-alpha-pinene, o-anisaldehyde, and cis-nerolidol. The half maximal inhibitory concentration (IC50) values of (+)-alpha-pinene, 3-carene, o-anisaldehyde, cis-nerolidol, and (-)-alpha-pinene against BxACE-2 were found to be 0.64, 1.41, 8.18, 8.53, 15.28, and 18.03mM, respectively. Coniferyl alcohol showed the strongest inhibition of BxACE-3 followed by (+)-alpha-pinene and cis-nerolidol.
ESTHER : Kang_2013_Pestic.Biochem.Physiol_105_50
PubMedSearch : Kang_2013_Pestic.Biochem.Physiol_105_50
PubMedID: 24238290
Gene_locus related to this paper: burxy-ACHE1 , burxy-ACHE2 , burxy-ACHE3

Title : The adhesion protein IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development - Woo_2013_J.Cell.Biol_201_929
Author(s) : Woo J , Kwon SK , Nam J , Choi S , Takahashi H , Krueger D , Park J , Lee Y , Bae JY , Lee D , Ko J , Kim H , Kim MH , Bae YC , Chang S , Craig AM , Kim E
Ref : Journal of Cell Biology , 201 :929 , 2013
Abstract : Synaptic adhesion molecules regulate diverse aspects of synapse formation and maintenance. Many known synaptic adhesion molecules localize at excitatory synapses, whereas relatively little is known about inhibitory synaptic adhesion molecules. Here we report that IgSF9b is a novel, brain-specific, homophilic adhesion molecule that is strongly expressed in GABAergic interneurons. IgSF9b was preferentially localized at inhibitory synapses in cultured rat hippocampal and cortical interneurons and was required for the development of inhibitory synapses onto interneurons. IgSF9b formed a subsynaptic domain distinct from the GABAA receptor- and gephyrin-containing domain, as indicated by super-resolution imaging. IgSF9b was linked to neuroligin 2, an inhibitory synaptic adhesion molecule coupled to gephyrin, via the multi-PDZ protein S-SCAM. IgSF9b and neuroligin 2 could reciprocally cluster each other. These results suggest a novel mode of inhibitory synaptic organization in which two subsynaptic domains, one containing IgSF9b for synaptic adhesion and the other containing gephyrin and GABAA receptors for synaptic transmission, are interconnected through S-SCAM and neuroligin 2.
ESTHER : Woo_2013_J.Cell.Biol_201_929
PubMedSearch : Woo_2013_J.Cell.Biol_201_929
PubMedID: 23751499

Title : Health Care Utilization and Costs Among Patients With AD With and Without Dysphagia - Tian_2013_Alzheimer.Dis.Assoc.Disord_27_138
Author(s) : Tian H , Abouzaid S , Sabbagh MN , Chen W , Gabriel S , Kahler KH , Kim E
Ref : Alzheimer Disease & Associated Disorders , 27 :138 , 2013
Abstract : OBJECTIVE: : To assess health care utilization and associated costs among patients with Alzheimer disease (AD), with and without dysphagia.
METHODS: : MarketScan Commercial and Medicare databases were analyzed. Patients with a diagnosis of AD with and without dysphagia between October 2006 and September 2010 were included. Acetylcholinesterase inhibitor usage, the number of outpatient and emergency room (ER) visits and hospitalizations, and associated health care costs were assessed. All variables were measured 1 year after the initial diagnosis of AD at the patient level. Patients with dysphagia were matched to patients without dysphagia using propensity score-matching (PSM) methods. Regression models were conducted to compare utilization and costs between the 2 groups.
RESULTS: : A total of 485 patients with dysphagia and 8492 patients without dysphagia were included. Before matching, patients with dysphagia were older (81.1 vs. 79.8 y), and had higher Charlson Comorbidity Index scores (2.4 vs. 1.7). After matching, all baseline covariates were not statistically different between the 2 groups. Multivariate regression results showed that patients with dysphagia had a higher likelihood of all-cause hospitalizations [odds ratio (OR)=2.26, 95% confidence interval (CI)=1.70-2.99, P=0.001] and all-cause ER visits (OR=1.45, 95% CI=1.12-1.87, P=0.007) compared with patients without dysphagia; they also had a higher likelihood for AD-related hospitalizations and ER visits. The difference in all-cause total health care, ER, and hospitalization costs between patients with and without dysphagia were $3620 (95% CI=$2863-$4375), $258 (95% CI=$241-$274), and $3547 (95% CI=$3325-$3770), respectively.
CONCLUSIONS: : This study suggests that patients with AD and dysphagia have higher health care utilization and costs compared with patients without dysphagia.
ESTHER : Tian_2013_Alzheimer.Dis.Assoc.Disord_27_138
PubMedSearch : Tian_2013_Alzheimer.Dis.Assoc.Disord_27_138
PubMedID: 22596081

Title : Effects of Treadmill Training on Limb Motor Function and Acetylcholinesterase Activity in Rats with Stroke - Kim_2013_J.Phys.Ther.Sci_25_1227
Author(s) : Kim G , Kim E
Ref : J Phys Ther Sci , 25 :1227 , 2013
Abstract : [Purpose] In the present study, we investigated the effects of treadmill training on limb motor function and acetylcholinesterase activity following focal cerebral ischemia injury. [Methods] Focal cerebral ischemia was examined in adult male Sprague-Dawley rats by using a middle cerebral artery occlusion model. Rats were randomly divided into 3 groups. Group I included untreated normal rats (n=12), Group II included untreated rats with focal cerebral ischemia (n=12), and Group III included rats that performed treadmill exercise (20 m/min) training after focal cerebral ischemia (n=12). We determined the limb placement test score for each rat on days 1,7, 14, and 21; acetylcholinesterase activity in the hippocampus was examined at the end of the experiment. [Results] We observed that the motor behavior index improved in the treadmill group, and hippocampal acetylcholinesterase activity was decreased. [Conclusion] These results indicated that treadmill training after focal cerebral ischemia exerts a neuroprotective effects against ischemic brain injury by improving motor performance and decreasing the levels of acetylcholinesterase activity. Furthermore, these results suggest that treadmill training at an appropriate intensity is critical for post-stroke rehabilitation.
ESTHER : Kim_2013_J.Phys.Ther.Sci_25_1227
PubMedSearch : Kim_2013_J.Phys.Ther.Sci_25_1227
PubMedID: 24259763

Title : Algal genomes reveal evolutionary mosaicism and the fate of nucleomorphs - Curtis_2012_Nature_492_59
Author(s) : Curtis BA , Tanifuji G , Burki F , Gruber A , Irimia M , Maruyama S , Arias MC , Ball SG , Gile GH , Hirakawa Y , Hopkins JF , Kuo A , Rensing SA , Schmutz J , Symeonidi A , Elias M , Eveleigh RJ , Herman EK , Klute MJ , Nakayama T , Obornik M , Reyes-Prieto A , Armbrust EV , Aves SJ , Beiko RG , Coutinho P , Dacks JB , Durnford DG , Fast NM , Green BR , Grisdale CJ , Hempel F , Henrissat B , Hoppner MP , Ishida K , Kim E , Koreny L , Kroth PG , Liu Y , Malik SB , Maier UG , McRose D , Mock T , Neilson JA , Onodera NT , Poole AM , Pritham EJ , Richards TA , Rocap G , Roy SW , Sarai C , Schaack S , Shirato S , Slamovits CH , Spencer DF , Suzuki S , Worden AZ , Zauner S , Barry K , Bell C , Bharti AK , Crow JA , Grimwood J , Kramer R , Lindquist E , Lucas S , Salamov A , McFadden GI , Lane CE , Keeling PJ , Gray MW , Grigoriev IV , Archibald JM
Ref : Nature , 492 :59 , 2012
Abstract : Cryptophyte and chlorarachniophyte algae are transitional forms in the widespread secondary endosymbiotic acquisition of photosynthesis by engulfment of eukaryotic algae. Unlike most secondary plastid-bearing algae, miniaturized versions of the endosymbiont nuclei (nucleomorphs) persist in cryptophytes and chlorarachniophytes. To determine why, and to address other fundamental questions about eukaryote-eukaryote endosymbiosis, we sequenced the nuclear genomes of the cryptophyte Guillardia theta and the chlorarachniophyte Bigelowiella natans. Both genomes have >21,000 protein genes and are intron rich, and B. natans exhibits unprecedented alternative splicing for a single-celled organism. Phylogenomic analyses and subcellular targeting predictions reveal extensive genetic and biochemical mosaicism, with both host- and endosymbiont-derived genes servicing the mitochondrion, the host cell cytosol, the plastid and the remnant endosymbiont cytosol of both algae. Mitochondrion-to-nucleus gene transfer still occurs in both organisms but plastid-to-nucleus and nucleomorph-to-nucleus transfers do not, which explains why a small residue of essential genes remains locked in each nucleomorph.
ESTHER : Curtis_2012_Nature_492_59
PubMedSearch : Curtis_2012_Nature_492_59
PubMedID: 23201678
Gene_locus related to this paper: guith-l1i9i5 , guith-l1k167 , guitc-l1jmn9

Title : Alternative splicing and developmental and hormonal regulation of porcine comparative gene identification-58 (CGI-58) mRNA - Li_2012_J.Anim.Sci_90_4346
Author(s) : Li X , Suh Y , Kim E , Moeller SJ , Lee K
Ref : J Anim Sci , 90 :4346 , 2012
Abstract : The process of lipolysis is essential for regulating the catabolism of cellular fat stores. Therefore, knowledge of lipolysis contributes to improving porcine production, such as reducing back fat, enhancing lean meat, and controlling marbling. Comparative gene identification-58 (CGI-58) plays an important role in the multi-enzyme-mediated process of lipolysis. It was identified as the co-activator of adipose triglyceride lipase (ATGL), which performs the first step in breaking down triacylglycerol and generating diacylglycerol and NEFA. We cloned and sequenced the CGI-58 cDNA and deduced the AA sequences in 3 breeds of swine (Duroc, Berkshire, and Landrace). Homologies were found with the human, mouse, and chicken for the lipid droplet binding domain, the alpha/beta hydrolase domain, and the lysophosphatidic acid acyltransferase (LPAAT) domain, which demonstrates conservation of CGI-58 across species. An alternatively spliced isoform with an exon 3 deletion was identified. Interestingly, this unique isoform contains the lipid droplet-binding domain but lacks the LPAAT domain due to an open reading frame (ORF) shift that creates a premature stop codon. Furthermore, porcine CGI-58 is expressed in multiple organs and tissues but is most predominant in adipose tissue. Porcine adipose and stromal-vascular (SV) cell fractionation reveals that CGI-58 and ATGL are highly expressed (P < 0.01) in mature adipocytes. The expressions of both CGI-58 and ATGL mRNA were found to increase (P < 0.05) at d 6 of SV cell culture, confirming their upregulation during adipogenesis and differentiation. Also, the results from in vitro cell culture showed that insulin decreased (P < 0.05) the expressions of both CGI-58 and ATGL in a dose-dependent manner. Overall, these results report the cDNA and AA sequences of porcine CGI-58 with identification of its unique alternatively spliced variant. The results of the study also reveal the developmental and hormonal regulation of porcine CGI-58 gene, which contributes to the understanding of the role of CGI-58 in lipid metabolism. These findings suggest that CGI-58 may be a new target for enhancing the quality of pork products as well as offering the potential of CGI-58 for human obesity treatment.
ESTHER : Li_2012_J.Anim.Sci_90_4346
PubMedSearch : Li_2012_J.Anim.Sci_90_4346
PubMedID: 22829614

Title : Genome Sequence of the ethene- and vinyl chloride-oxidizing actinomycete Nocardioides sp. strain JS614 - Coleman_2011_J.Bacteriol_193_3399
Author(s) : Coleman NV , Wilson NL , Barry K , Brettin TS , Bruce DC , Copeland A , Dalin E , Detter JC , Del Rio TG , Goodwin LA , Hammon NM , Han S , Hauser LJ , Israni S , Kim E , Kyrpides N , Land ML , Lapidus A , Larimer FW , Lucas S , Pitluck S , Richardson P , Schmutz J , Tapia R , Thompson S , Tice HN , Spain JC , Gossett JG , Mattes TE
Ref : Journal of Bacteriology , 193 :3399 , 2011
Abstract : Nocardioides sp. strain JS614 grows on ethene and vinyl chloride (VC) as sole carbon and energy sources and is of interest for bioremediation and biocatalysis. Sequencing of the complete genome of JS614 provides insight into the genetic basis of alkene oxidation, supports ongoing research into the physiology and biochemistry of growth on ethene and VC, and provides biomarkers to facilitate detection of VC/ethene oxidizers in the environment. This is the first genome sequence from the genus Nocardioides and the first genome of a VC/ethene-oxidizing bacterium.
ESTHER : Coleman_2011_J.Bacteriol_193_3399
PubMedSearch : Coleman_2011_J.Bacteriol_193_3399
PubMedID: 21551312
Gene_locus related to this paper: nocsj-a1sil5

Title : DAG lipase activity is necessary for TRP channel regulation in Drosophila photoreceptors - Leung_2008_Neuron_58_884
Author(s) : Leung HT , Tseng-Crank J , Kim E , Mahapatra C , Shino S , Zhou Y , An L , Doerge RW , Pak WL
Ref : Neuron , 58 :884 , 2008
Abstract : In Drosophila, a phospholipase C-mediated signaling cascade links photoexcitation of rhodopsin to the opening of the TRP/TRPL channels. A lipid product of the cascade, diacylglycerol (DAG) and its metabolite(s), polyunsaturated fatty acids (PUFAs), have both been proposed as potential excitatory messengers. A crucial enzyme in the understanding of this process is likely to be DAG lipase (DAGL). However, DAGLs that might fulfill this role have not been previously identified in any organism. In this work, the Drosophila DAGL gene, inaE, has been identified from mutants that are defective in photoreceptor responses to light. The inaE-encoded protein isoforms show high sequence similarity to known mammalian DAG lipases, exhibit DAG lipase activity in vitro, and are highly expressed in photoreceptors. Analyses of norpA inaE double mutants and severe inaE mutants show that normal DAGL activity is required for the generation of physiologically meaningful photoreceptor responses.
ESTHER : Leung_2008_Neuron_58_884
PubMedSearch : Leung_2008_Neuron_58_884
PubMedID: 18579079
Gene_locus related to this paper: drome-CG33174

Title : Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction - Anderson_2008_J.Bacteriol_190_2957
Author(s) : Anderson I , Rodriguez J , Susanti D , Porat I , Reich C , Ulrich LE , Elkins JG , Mavromatis K , Lykidis A , Kim E , Thompson LS , Nolan M , Land M , Copeland A , Lapidus A , Lucas S , Detter C , Zhulin IB , Olsen GJ , Whitman W , Mukhopadhyay B , Bristow J , Kyrpides N
Ref : Journal of Bacteriology , 190 :2957 , 2008
Abstract : We report the complete genome of Thermofilum pendens, a deeply branching, hyperthermophilic member of the order Thermoproteales in the archaeal kingdom Crenarchaeota. T. pendens is a sulfur-dependent, anaerobic heterotroph isolated from a solfatara in Iceland. It is an extracellular commensal, requiring an extract of Thermoproteus tenax for growth, and the genome sequence reveals that biosynthetic pathways for purines, most amino acids, and most cofactors are absent. In fact, T. pendens has fewer biosynthetic enzymes than obligate intracellular parasites, although it does not display other features that are common among obligate parasites and thus does not appear to be in the process of becoming a parasite. It appears that T. pendens has adapted to life in an environment rich in nutrients. T. pendens was known previously to utilize peptides as an energy source, but the genome revealed a substantial ability to grow on carbohydrates. T. pendens is the first crenarchaeote and only the second archaeon found to have a transporter of the phosphotransferase system. In addition to fermentation, T. pendens may obtain energy from sulfur reduction with hydrogen and formate as electron donors. It may also be capable of sulfur-independent growth on formate with formate hydrogen lyase. Additional novel features are the presence of a monomethylamine:corrinoid methyltransferase, the first time that this enzyme has been found outside the Methanosarcinales, and the presence of a presenilin-related protein. The predicted highly expressed proteins do not include proteins encoded by housekeeping genes and instead include ABC transporters for carbohydrates and peptides and clustered regularly interspaced short palindromic repeat-associated proteins.
ESTHER : Anderson_2008_J.Bacteriol_190_2957
PubMedSearch : Anderson_2008_J.Bacteriol_190_2957
PubMedID: 18263724
Gene_locus related to this paper: thepd-a1s004

Title : Nuclear translocation of CAM-associated protein activates transcription for long-term facilitation in Aplysia - Lee_2007_Cell_129_801
Author(s) : Lee SH , Lim CS , Park H , Lee JA , Han JH , Kim H , Cheang YH , Lee YS , Ko HG , Jang DH , Miniaci MC , Bartsch D , Kim E , Bailey CH , Kandel ER , Kaang BK
Ref : Cell , 129 :801 , 2007
Abstract : Repeated pulses of serotonin (5-HT) induce long-term facilitation (LTF) of the synapses between sensory and motor neurons of the gill-withdrawal reflex in Aplysia. To explore how apCAM downregulation at the plasma membrane and CREB-mediated transcription in the nucleus, both of which are required for the formation of LTF, might relate to each other, we cloned an apCAM-associated protein (CAMAP) by yeast two-hybrid screening. We found that 5-HT signaling at the synapse activates PKA which in turn phosphorylates CAMAP to induce the dissociation of CAMAP from apCAM and the subsequent translocation of CAMAP into the nucleus of sensory neurons. In the nucleus, CAMAP acts as a transcriptional coactivator for CREB1 and is essential for the activation of ApC/EBP required for the initiation of LTF. Combined, our data suggest that CAMAP is a retrograde signaling component that translocates from activated synapses to the nucleus during synapse-specific LTF.
ESTHER : Lee_2007_Cell_129_801
PubMedSearch : Lee_2007_Cell_129_801
PubMedID: 17512412

Title : Identification, cloning, and characterization of a multicomponent biphenyl dioxygenase from Sphingobium yanoikuyae B1 - Chadhain_2007_J.Ind.Microbiol.Biotechnol_34_605
Author(s) : Chadhain SM , Moritz EM , Kim E , Zylstra GJ
Ref : J Ind Microbiol Biotechnol , 34 :605 , 2007
Abstract : Sphingobium yanoikuyae B1 utilizes both polycyclic aromatic hydrocarbons (biphenyl, naphthalene, and phenanthrene) and monocyclic aromatic hydrocarbons (toluene, m- and p-xylene) as its sole source of carbon and energy for growth. The majority of the genes for these intertwined monocyclic and polycyclic aromatic pathways are grouped together on a 39 kb fragment of chromosomal DNA. However, this gene cluster is missing several genes encoding essential enzymatic steps in the aromatic degradation pathway, most notably the genes encoding the oxygenase component of the initial polycyclic aromatic hydrocarbon (PAH) dioxygenase. Transposon mutagenesis of strain B1 yielded a mutant blocked in the initial oxidation of PAHs. The transposon insertion point was sequenced and a partial gene sequence encoding an oxygenase component of a putative PAH dioxygenase identified. A cosmid clone from a genomic library of S. yanoikuyae B1 was identified which contains the complete putative PAH oxygenase gene sequence. Separate clones expressing the genes encoding the electron transport components (ferredoxin and reductase) and the PAH dioxygenase were constructed. Incubation of cells expressing the dioxygenase enzyme system with biphenyl or naphthalene resulted in production of the corresponding cis-dihydrodiol confirming PAH dioxygenase activity. This demonstrates that a single multicomponent dioxygenase enzyme is involved in the initial oxidation of both biphenyl and naphthalene in S. yanoikuyae B1.
ESTHER : Chadhain_2007_J.Ind.Microbiol.Biotechnol_34_605
PubMedSearch : Chadhain_2007_J.Ind.Microbiol.Biotechnol_34_605
PubMedID: 17647036
Gene_locus related to this paper: psepa-q6qhu7

Title : Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding - Bourne_2006_J.Biol.Chem_281_29256
Author(s) : Bourne Y , Radic Z , Sulzenbacher G , Kim E , Taylor P , Marchot P
Ref : Journal of Biological Chemistry , 281 :29256 , 2006
Abstract : Hydrolysis of acetylcholine catalyzed by acetylcholinesterase (AChE), one of the most efficient enzymes in nature, occurs at the base of a deep and narrow active center gorge. At the entrance of the gorge, the peripheral anionic site provides a binding locus for allosteric ligands, including substrates. To date, no structural information on substrate entry to the active center from the peripheral site of AChE or its subsequent egress has been reported. Complementary crystal structures of mouse AChE and an inactive mouse AChE mutant with a substituted catalytic serine (S203A), in various complexes with four substrates (acetylcholine, acetylthiocholine, succinyldicholine, and butyrylthiocholine), two non-hydrolyzable substrate analogues (m-(N,N,N-trimethylammonio)-trifluoroacetophenone and 4-ketoamyltrimethylammonium), and one reaction product (choline) were solved in the 2.05-2.65-A resolution range. These structures, supported by binding and inhibition data obtained on the same complexes, reveal the successive positions and orientations of the substrates bound to the peripheral site and proceeding within the gorge toward the active site, the conformations of the presumed transition state for acylation and the acyl-enzyme intermediate, and the positions and orientations of the dissociating and egressing products. Moreover, the structures of the AChE mutant in complexes with acetylthiocholine and succinyldicholine reveal additional substrate binding sites on the enzyme surface, distal to the gorge entry. Hence, we provide a comprehensive set of structural snapshots of the steps leading to the intermediates of catalysis and the potential regulation by substrate binding to various allosteric sites at the enzyme surface.
ESTHER : Bourne_2006_J.Biol.Chem_281_29256
PubMedSearch : Bourne_2006_J.Biol.Chem_281_29256
PubMedID: 16837465
Gene_locus related to this paper: mouse-ACHE

Title : A case with cholinesterase inhibitor responsive asymmetric posterior cortical atrophy - Kim_2005_Clin.Neurol.Neurosurg_108_97
Author(s) : Kim E , Lee Y , Lee J , Han SH
Ref : Clin Neurol Neurosurg , 108 :97 , 2005
Abstract : A 55-year-old right-handed woman presented initially with mild amnestic and depressive episodes but slowly developed progressive neurobehavioral symptoms, indicative of posterior cortical atrophy in ensuing years. A more detailed neurobehavioral test suggested predominant right temporo-parietal dysfunction with executive functional deficits. SPECT and MRI findings revealed right unilateral temporo-parietal involvement. Cholinesterase inhibitor administration led to amelioration of symptoms. We suggest that cases of posterior cortical atrophy or visual variant of Alzheimer's disease may be responsive to cholinesterase inhibitor therapy; however, the observation in a single case should be confirmed on larger populations in a clinical trial design with placebo control.
ESTHER : Kim_2005_Clin.Neurol.Neurosurg_108_97
PubMedSearch : Kim_2005_Clin.Neurol.Neurosurg_108_97
PubMedID: 16311158

Title : Synthesis and melanin biosynthesis inhibitory activity of (+\/-)-terrein produced by Penicillium sp. 20135 - Lee_2005_Bioorg.Med.Chem.Lett_15_471
Author(s) : Lee S , Kim WG , Kim E , Ryoo IJ , Lee HK , Kim JN , Jung SH , Yoo ID
Ref : Bioorganic & Medicinal Chemistry Lett , 15 :471 , 2005
Abstract : Terrein was isolated from Penicillium sp. 20135, prepared by a practical synthetic way, and evaluated first time for its melanin biosynthesis inhibitory activity.
ESTHER : Lee_2005_Bioorg.Med.Chem.Lett_15_471
PubMedSearch : Lee_2005_Bioorg.Med.Chem.Lett_15_471
PubMedID: 15603975
Gene_locus related to this paper: aspte-AT1

Title : Identification of a novel dioxygenase involved in metabolism of o-xylene, toluene, and ethylbenzene by Rhodococcus sp. strain DK17 - Kim_2004_Appl.Environ.Microbiol_70_7086
Author(s) : Kim D , Chae JC , Zylstra GJ , Kim YS , Kim SK , Nam MH , Kim YM , Kim E
Ref : Applied Environmental Microbiology , 70 :7086 , 2004
Abstract : Rhodococcus sp. strain DK17 is able to grow on o-xylene, benzene, toluene, and ethylbenzene. DK17 harbors at least two megaplasmids, and the genes encoding the initial steps in alkylbenzene metabolism are present on the 330-kb pDK2. The genes encoding alkylbenzene degradation were cloned in a cosmid clone and sequenced completely to reveal 35 open reading frames (ORFs). Among the ORFs, we identified two nearly exact copies (one base difference) of genes encoding large and small subunits of an iron sulfur protein terminal oxygenase that are 6 kb apart from each other. Immediately downstream of one copy of the dioxygenase genes (akbA1a and akbA2a) is a gene encoding a dioxygenase ferredoxin component (akbA3), and downstream of the other copy (akbA1b and akbA2b) are genes putatively encoding a meta-cleavage pathway. RT-PCR experiments show that the two copies of the dioxygenase genes are operonic with the downstream putative catabolic genes and that both operons are induced by o-xylene. When expressed in Escherichia coli, AkbA1a-AkbA2a-AkbA3 transformed o-xylene into 2,3- and 3,4-dimethylphenol. These were apparently derived from an unstable o-xylene cis-3,4-dihydrodiol, which readily dehydrates. This indicates a single point of attack of the dioxygenase on the aromatic ring. In contrast, attack of AkbA1a-AkbA2a-AkbA3 on ethylbenzene resulted in the formation of two different cis-dihydrodiols resulting from an oxidation at the 2,3 and the 3,4 positions on the aromatic ring, respectively.
ESTHER : Kim_2004_Appl.Environ.Microbiol_70_7086
PubMedSearch : Kim_2004_Appl.Environ.Microbiol_70_7086
PubMedID: 15574904
Gene_locus related to this paper: rhosp-bphd

Title : Intrinsic tryptophan fluorescence of cholinesterases. -
Author(s) : Radic Z , Kim E , Taylor P
Ref : Cholinergic Mechanisms, CRC Press :171 , 2004

Title : Monitoring cholinesterase - ligand interactions -
Author(s) : Radic Z , Kim E , Taylor P
Ref : In: Cholinesterases in the Second Millennium: Biomolecular and Pathological Aspects , (Inestrosa NC, Campos EO) P. Universidad Catolica de Chile-FONDAP Biomedicina :201 , 2004

Title : Naringenin from Citrus junos has an inhibitory effect on acetylcholinesterase and a mitigating effect on amnesia - Heo_2004_Dement.Geriatr.Cogn.Disord_17_151
Author(s) : Heo HJ , Kim MJ , Lee JM , Choi SJ , Cho HY , Hong B , Kim HK , Kim E , Shin DH
Ref : Dementia & Geriatric Cognitive Disorders , 17 :151 , 2004
Abstract : This study was performed to identify safe and more effective acetylcholinesterase (AChE) inhibitors in the treatment of Alzheimer's disease. The total methanol extract of Citrus junos had a significant inhibitory effect on AChE in vitro. By sequential fractionation of C.junos, the active component was finally identified as naringenin. Naringenin inhibited AChE activity in a dose-dependent manner. In this study, we also evaluated the anti-amnesic activity of naringenin, a major flavanone constituent isolated from C. junos, in vivo using ICR mice with amnesia induced by scopolamine (1 mg/kg body weight). Naringenin, when administered to mice at 4.5 mg/kg body weight, significantly ameliorated scopolamine-induced amnesia as measured in both the passive avoidance and the Y-maze test. These results suggest that naringenin may be a useful chemopreventive agent against Alzheimer's disease.
ESTHER : Heo_2004_Dement.Geriatr.Cogn.Disord_17_151
PubMedSearch : Heo_2004_Dement.Geriatr.Cogn.Disord_17_151
PubMedID: 14739537

Title : Effects of AF102B and tacrine on delayed match-to-sample in monkeys - O'Neill_1998_Prog.Neuropsychopharmacol.Biol.Psychiatry_22_665
Author(s) : O'Neill J , Fitten LJ , Siembieda D , Halgren E , Kim E , Fisher A , Perryman K
Ref : Prog Neuropsychopharmacol Biological Psychiatry , 22 :665 , 1998
Abstract : 1. Object working memory, a function which declines in aging and dementia, was tested in young and aged pretrained monkeys using a delayed match-to-sample task. 2. During drug treatment, monkeys were given the m 1 muscarinic agonist AF102B (0.1-2.1 mg/kg i.m.), the cholinesterase inhibitor tacrine (0.5-2.0 mg/kg p.o.), or vehicle controls in a repeated measures design to assess putative cognitive enhancement. 3. Both agents improved task performance in both young and aged monkeys, AF102B yielding equivalent or greater, and less variable, improvement than tacrine. 4. AF102B may represent a low-toxicity alternative to tacrine for the treatment of age-related memory disorders.
ESTHER : O'Neill_1998_Prog.Neuropsychopharmacol.Biol.Psychiatry_22_665
PubMedSearch : O'Neill_1998_Prog.Neuropsychopharmacol.Biol.Psychiatry_22_665
PubMedID: 9682279

Title : Synaptic clustering of the cell adhesion molecule fasciclin II by discs-large and its role in the regulation of presynaptic structure - Thomas_1997_Neuron_19_787
Author(s) : Thomas U , Kim E , Kuhlendahl S , Koh YH , Gundelfinger ED , Sheng M , Garner CC , Budnik V
Ref : Neuron , 19 :787 , 1997
Abstract : The cell adhesion molecule Fasciclin II (FASII) is involved in synapse development and plasticity. Here we provide genetic and biochemical evidence that proper localization of FASII at type I glutamatergic synapses of the Drosophila neuromuscular junction is mediated by binding between the intracellular tSXV bearing C-terminal tail of FASII and the PDZ1-2 domains of Discs-Large (DLG). Moreover, mutations in fasII and/or dlg have similar effects on presynaptic ultrastructure, suggesting their functional involvement in a common developmental pathway. DLG can directly mediate a biochemical complex and a macroscopic cluster of FASII and Shaker K+ channels in heterologous cells. These results indicate a central role for DLG in the structural organization and downstream signaling mechanisms of cell adhesion molecules and ion channels at synapses.
ESTHER : Thomas_1997_Neuron_19_787
PubMedSearch : Thomas_1997_Neuron_19_787
PubMedID: 9354326

Title : Genetic structures of the genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase from biphenyl- and 4-chlorobiphenyl-degrading Pseudomonas sp. strain DJ-12 - Kim_1996_Appl.Environ.Microbiol_62_262
Author(s) : Kim E , Kim Y , Kim CK
Ref : Applied Environmental Microbiology , 62 :262 , 1996
Abstract : The pcbC and pcbD genes of Pseudomonas sp. strain DJ-12, a natural isolate degrading biphenyl and 4-chlorobiphenyl, encode the 2,3-dihydroxybiphenyl 1,2-dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase, respectively. The two genes were sequenced and appear to be present in the order pcbD-pcbC as an operon.
ESTHER : Kim_1996_Appl.Environ.Microbiol_62_262
PubMedSearch : Kim_1996_Appl.Environ.Microbiol_62_262
PubMedID: 8572703
Gene_locus related to this paper: psesp-pcbD