Misra M

References (14)

Title : Formulation and In-vivo Pharmacokinetic Consideration of Intranasal Microemulsion and Mucoadhesive Microemulsion of Rivastigmine for Brain Targeting - Shah_2018_Pharm.Res_35_8
Author(s) : Shah B , Khunt D , Misra M , Padh H
Ref : Pharm Res , 35 :8 , 2018
Abstract : PURPOSE: Presence of tight junctions in blood brain barrier (BBB) pose a major hurdle for delivery of drug and severely affects adequate therapeutic concentration to reach the brain. In present work, we have selected Rivastigmine hydrogen tartrate (RHT), a reversible cholinesterase inhibitor, which exhibits extensive first-pass metabolism, resulting in limited absolute bioavailability (36%). RHT shows extremely low aqueous solubility and poor penetration, resulting in inadequate concentration reaching the brain, thus necessitating frequent oral dosing. To overcome these problems of RHT, microemulsion (ME) and mucoadhesive microemulsion (MME) of RHT were formulated for brain targeting via intranasal delivery route and compared on the basis of in vivo pharmacokinetics. METHODS: ME and MME formulations containing RHT were developed by water titration method. Characterization of ME and MME was done for various physicochemical parameters, nasal spray pattern, and in vivo pharmacokinetics quantitatively and qualitatively (gamma scintigraphy studies). RESULTS: The developed ME and MME were transparent having globule size approximately in the range of 53-55 nm. Pharmacokinetic studies showed higher values for Cmax and DTP for intranasal RHT: CH-ME over RHT-ME, thus indicating the effect of chitosan in modulating tight junctions, thereby enhanced paracellular transport of RHT. CONCLUSION: Gamma scintigraphy and in vivo pharmacokinetic study suggested enhanced RHT concentration, upon intranasal administration of RHT:CH-ME, compare with other groups administered formulations intranasally. These findings suggested the potential of non-invasive intranasal route for brain delivery, especially for therapeutics, facing challenges in oral administration.
ESTHER : Shah_2018_Pharm.Res_35_8
PubMedSearch : Shah_2018_Pharm.Res_35_8
PubMedID: 29294189

Title : Nose to brain microemulsion-based drug delivery system of rivastigmine: formulation and ex-vivo characterization - Shah_2015_Drug.Deliv_22_918
Author(s) : Shah BM , Misra M , Shishoo CJ , Padh H
Ref : Drug Deliv , 22 :918 , 2015
Abstract : Alzheimer's disease (AD) is a progressive neurodegenerative disorder leading to irreversible loss of neurons, cognition and formation of abnormal protein aggregates. Rivastigmine, a reversible cholinesterase inhibitor used for the treatment of AD, undergoes extensive first-pass metabolism, thus limiting its absolute bioavailability to only 36% after 3-mg dose. Due to extreme aqueous solubility, rivastigmine shows poor penetration and lesser concentration in the brain thus requiring frequent oral dosing. This investigation was aimed to formulate microemulsion (ME) and mucoadhesive microemulsions (MMEs) of rivastigmine for nose to brain delivery and to compare percentage drug diffused for both systems using in-vitro and ex-vivo study. Rivastigmine-loaded ME and MMEs were prepared by titration method and characterized for drug content, globule size distribution, zeta potential, pH, viscosity and nasal ciliotoxicity study. Rivastigmine-loaded ME system containing 8% w/w Capmul MCM EP, 44% w/w Labrasol:Transcutol-P (1:1) and 48% w/w distilled water was formulated, whereas 0.3% w/w chitosan (CH) and cetyl trimethyl ammonium bromide (as mucoadhesive agents) were used to formulate MMEs, respectively. ME and MMEs formulations were transparent with drug content, globule size and zeta potential in the range of 98.59% to 99.43%, 53.8 nm to 55.4 nm and -2.73 mV to 6.52 mV, respectively. MME containing 0.3% w/w CH followed Higuchi model (r(2) = 0.9773) and showed highest diffusion coefficient. It was free from nasal ciliotoxicity and stable for three months. However, the potential of developed CH-based MME for nose to brain delivery of rivastigmine can only be established after in-vivo and biodistribution study.
ESTHER : Shah_2015_Drug.Deliv_22_918
PubMedSearch : Shah_2015_Drug.Deliv_22_918
PubMedID: 24467601

Title : Genome sequence of the thermophilic fresh-water bacterium Spirochaeta caldaria type strain (H1(T)), reclassification of Spirochaeta caldaria, Spirochaeta stenostrepta, and Spirochaeta zuelzerae in the genus Treponema as Treponema caldaria comb. nov., Treponema stenostrepta comb. nov., and Treponema zuelzerae comb. nov., and emendation of the genus Treponema - Abt_2013_Stand.Genomic.Sci_8_88
Author(s) : Abt B , Goker M , Scheuner C , Han C , Lu M , Misra M , Lapidus A , Nolan M , Lucas S , Hammon N , Deshpande S , Cheng JF , Tapia R , Goodwin LA , Pitluck S , Liolios K , Pagani I , Ivanova N , Mavromatis K , Mikhailova N , Huntemann M , Pati A , Chen A , Palaniappan K , Land M , Hauser L , Jeffries CD , Rohde M , Spring S , Gronow S , Detter JC , Bristow J , Eisen JA , Markowitz V , Hugenholtz P , Kyrpides NC , Woyke T , Klenk HP
Ref : Stand Genomic Sci , 8 :88 , 2013
Abstract : Spirochaeta caldaria Pohlschroeder et al. 1995 is an obligately anaerobic, spiral-shaped bacterium that is motile via periplasmic flagella. The type strain, H1(T), was isolated in 1990 from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA, and is of interest because it enhances the degradation of cellulose when grown in co-culture with Clostridium thermocellum. Here we provide a taxonomic re-evaluation for S. caldaria based on phylogenetic analyses of 16S rRNA sequences and whole genomes, and propose the reclassification of S. caldaria and two other Spirochaeta species as members of the emended genus Treponema. Whereas genera such as Borrelia and Sphaerochaeta possess well-distinguished genomic features related to their divergent lifestyles, the physiological and functional genomic characteristics of Spirochaeta and Treponema appear to be intermixed and are of little taxonomic value. The 3,239,340 bp long genome of strain H1(T) with its 2,869 protein-coding and 59 RNA genes is a part of the G enomic E ncyclopedia of Bacteria and Archaea project.
ESTHER : Abt_2013_Stand.Genomic.Sci_8_88
PubMedSearch : Abt_2013_Stand.Genomic.Sci_8_88
PubMedID: 23961314
Gene_locus related to this paper: trech-f8f1l1

Title : Complete genome sequence of Cellulophaga algicola type strain (IC166) - Abt_2011_Stand.Genomic.Sci_4_72
Author(s) : Abt B , Lu M , Misra M , Han C , Nolan M , Lucas S , Hammon N , Deshpande S , Cheng JF , Tapia R , Goodwin L , Pitluck S , Liolios K , Pagani I , Ivanova N , Mavromatis K , Ovchinikova G , Pati A , Chen A , Palaniappan K , Land M , Hauser L , Chang YJ , Jeffries CD , Detter JC , Brambilla E , Rohde M , Tindall BJ , Goker M , Woyke T , Bristow J , Eisen JA , Markowitz V , Hugenholtz P , Kyrpides NC , Klenk HP , Lapidus A
Ref : Stand Genomic Sci , 4 :72 , 2011
Abstract : Cellulophaga algicola Bowman 2000 belongs to the family Flavobacteriaceae within the phylum 'Bacteroidetes' and was isolated from Melosira collected from the Eastern Antarctic coastal zone. The species is of interest because its members produce a wide range of extracellular enzymes capable of degrading proteins and polysaccharides with temperature optima of 20-30 degrees C. This is the first completed genome sequence of a member of the genus Cellulophaga. The 4,888,353 bp long genome with its 4,285 protein-coding and 62 RNA genes consists of one circular chromosome and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
ESTHER : Abt_2011_Stand.Genomic.Sci_4_72
PubMedSearch : Abt_2011_Stand.Genomic.Sci_4_72
PubMedID: 21475589
Gene_locus related to this paper: celad-e6x4e5 , celad-e6x420 , celad-e6x777 , celad-e6xbe7

Title : Comparative genome sequence analysis underscores mycoparasitism as the ancestral life style of Trichoderma - Kubicek_2011_Genome.Biol_12_R40
Author(s) : Kubicek CP , Herrera-Estrella A , Seidl-Seiboth V , Martinez DA , Druzhinina IS , Thon M , Zeilinger S , Casas-Flores S , Horwitz BA , Mukherjee PK , Mukherjee M , Kredics L , Alcaraz LD , Aerts A , Antal Z , Atanasova L , Cervantes-Badillo MG , Challacombe J , Chertkov O , McCluskey K , Coulpier F , Deshpande N , von Dohren H , Ebbole DJ , Esquivel-Naranjo EU , Fekete E , Flipphi M , Glaser F , Gomez-Rodriguez EY , Gruber S , Han C , Henrissat B , Hermosa R , Hernandez-Onate M , Karaffa L , Kosti I , Le Crom S , Lindquist E , Lucas S , Lubeck M , Lubeck PS , Margeot A , Metz B , Misra M , Nevalainen H , Omann M , Packer N , Perrone G , Uresti-Rivera EE , Salamov A , Schmoll M , Seiboth B , Shapiro H , Sukno S , Tamayo-Ramos JA , Tisch D , Wiest A , Wilkinson HH , Zhang M , Coutinho PM , Kenerley CM , Monte E , Baker SE , Grigoriev IV
Ref : Genome Biol , 12 :R40 , 2011
Abstract : BACKGROUND: Mycoparasitism, a lifestyle where one fungus is parasitic on another fungus, has special relevance when the prey is a plant pathogen, providing a strategy for biological control of pests for plant protection. Probably, the most studied biocontrol agents are species of the genus Hypocrea/Trichoderma.
RESULTS: Here we report an analysis of the genome sequences of the two biocontrol species Trichoderma atroviride (teleomorph Hypocrea atroviridis) and Trichoderma virens (formerly Gliocladium virens, teleomorph Hypocrea virens), and a comparison with Trichoderma reesei (teleomorph Hypocrea jecorina). These three Trichoderma species display a remarkable conservation of gene order (78 to 96%), and a lack of active mobile elements probably due to repeat-induced point mutation. Several gene families are expanded in the two mycoparasitic species relative to T. reesei or other ascomycetes, and are overrepresented in non-syntenic genome regions. A phylogenetic analysis shows that T. reesei and T. virens are derived relative to T. atroviride. The mycoparasitism-specific genes thus arose in a common Trichoderma ancestor but were subsequently lost in T. reesei.
CONCLUSIONS: The data offer a better understanding of mycoparasitism, and thus enforce the development of improved biocontrol strains for efficient and environmentally friendly protection of plants.
ESTHER : Kubicek_2011_Genome.Biol_12_R40
PubMedSearch : Kubicek_2011_Genome.Biol_12_R40
PubMedID: 21501500
Gene_locus related to this paper: hypai-g9nem6 , hypai-g9ng36 , hypai-g9ngu2 , hypai-g9nks5 , hypai-g9nks6 , hypai-g9nqe5 , hypai-g9nqk5 , hypai-g9nrx6 , hypai-g9nsx1 , hypai-g9ntn3 , hypai-g9nzc9 , hypai-g9nzd7 , hypai-g9p1t1 , hypai-g9p1v2 , hypai-g9p2n8 , hypai-g9p4z2 , hypai-g9p878 , hypai-g9pa17 , hypai-g9pbz9 , hypvg-g9mem8 , hypvg-g9mg52 , hypvg-g9mga2 , hypvg-g9mhi3 , hypvg-g9mjc7 , hypvg-g9mk44 , hypvg-g9mms1 , hypvg-g9mnf0 , hypvg-g9mng3 , hypvg-g9mpt0 , hypvg-g9mrp9 , hypvg-g9ms16 , hypvg-g9ms32 , hypvg-g9msv5 , hypvg-g9muh6 , hypvg-g9muk0 , hypvg-g9mwe2 , hypvg-g9my79 , hypvg-g9n0p7 , hypvg-g9n2g3 , hypvg-g9n2g4 , hypvg-g9n4k5 , hypvg-g9n9n0 , hypvg-g9n561 , hypvg-g9n988 , hypvg-g9nb12 , hypvg-g9nb54 , hypvg-g9nbh8 , hypai-g9npz7 , hypai-g9njw6 , hypvg-g9mx08 , hypvg-g9mlt2 , hypai-g9p4j3 , hypvg-g9nbd3 , hypai-g9nxf6 , hypvg-g9n3y9 , hypvg-g9mgs4 , hypai-g9p6m2 , hypvg-g9my62 , hypvg-g9nbv2 , hypvg-g9my22 , hypai-g9p2e2 , hypai-g9p596 , hypai-g9nf87 , hypvg-g9me87 , hypvg-g9ndn9 , hypai-g9niy5 , hypai-g9ntx6 , hypvg-g9n3e7 , hypai-g9nu29 , hypvg-g9n2z0 , hypvg-g9ndf4 , 9hypo-a0a2p4zt82 , hypvg-g9n0g0 , hypvg-g9muj2 , hypvg-g9mud0 , hypai-g9nkx5

Title : Complete genome sequence of Segniliparus rotundus type strain (CDC 1076) - Sikorski_2010_Stand.Genomic.Sci_2_203
Author(s) : Sikorski J , Lapidus A , Copeland A , Misra M , Glavina Del Rio T , Nolan M , Lucas S , Chen F , Tice H , Cheng JF , Jando M , Schneider S , Bruce D , Goodwin L , Pitluck S , Liolios K , Mikhailova N , Pati A , Ivanova N , Mavromatis K , Chen A , Palaniappan K , Chertkov O , Land M , Hauser L , Chang YJ , Jeffries CD , Brettin T , Detter JC , Han C , Rohde M , Goker M , Bristow J , Eisen JA , Markowitz V , Hugenholtz P , Kyrpides NC , Klenk HP
Ref : Stand Genomic Sci , 2 :203 , 2010
Abstract : Segniliparus rotundus Butler 2005 is the type species of the genus Segniliparus, which is currently the only genus in the corynebacterial family Segniliparaceae. This family is of large interest because of a novel late-emerging genus-specific mycolate pattern. The type strain has been isolated from human sputum and is probably an opportunistic pathogen. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first completed genome sequence of the family Segniliparaceae. The 3,157,527 bp long genome with its 3,081 protein-coding and 52 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project.
ESTHER : Sikorski_2010_Stand.Genomic.Sci_2_203
PubMedSearch : Sikorski_2010_Stand.Genomic.Sci_2_203
PubMedID: 21304703
Gene_locus related to this paper: segrd-d6z8m1 , segrd-d6z8p5 , segrd-d6z9l9 , segrd-d6za06 , segrd-d6zaa6 , segrd-d6zav0 , segrd-d6zbl4 , segrd-d6zbs4 , segrd-d6zc43 , segrd-d6zca1 , segrd-d6zcn6 , segrd-d6zdf7 , segrd-d6zds6 , segrd-d6zdt4 , segrd-d6zdz3 , segrd-d6zed7 , segrd-d6zej1 , segrd-d6zfg4 , segrd-d6zfr6 , segrd-d6za90 , segrd-d6za91 , segrd-d6zd15 , segrd-d6zcg9 , segrd-d6zb77

Title : Complete genome sequence of Thermocrinis albus type strain (HI 11\/12) - Wirth_2010_Stand.Genomic.Sci_2_194
Author(s) : Wirth R , Sikorski J , Brambilla E , Misra M , Lapidus A , Copeland A , Nolan M , Lucas S , Chen F , Tice H , Cheng JF , Han C , Detter JC , Tapia R , Bruce D , Goodwin L , Pitluck S , Pati A , Anderson I , Ivanova N , Mavromatis K , Mikhailova N , Chen A , Palaniappan K , Bilek Y , Hader T , Land M , Hauser L , Chang YJ , Jeffries CD , Tindall BJ , Rohde M , Goker M , Bristow J , Eisen JA , Markowitz V , Hugenholtz P , Kyrpides NC , Klenk HP
Ref : Stand Genomic Sci , 2 :194 , 2010
Abstract : Thermocrinis albus Eder and Huber 2002 is one of three species in the genus Thermocrinis in the family Aquificaceae. Members of this family have become of significant interest because of their involvement in global biogeochemical cycles in high-temperature ecosystems. This interest had already spurred several genome sequencing projects for members of the family. We here report the first completed genome sequence a member of the genus Thermocrinis and the first type strain genome from a member of the family Aquificaceae. The 1,500,577 bp long genome with its 1,603 protein-coding and 47 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project.
ESTHER : Wirth_2010_Stand.Genomic.Sci_2_194
PubMedSearch : Wirth_2010_Stand.Genomic.Sci_2_194
PubMedID: 21304702
Gene_locus related to this paper: theah-d3smz6

Title : Complete genome sequence of Methanothermus fervidus type strain (V24S) - Anderson_2010_Stand.Genomic.Sci_3_315
Author(s) : Anderson I , Djao OD , Misra M , Chertkov O , Nolan M , Lucas S , Lapidus A , Del Rio TG , Tice H , Cheng JF , Tapia R , Han C , Goodwin L , Pitluck S , Liolios K , Ivanova N , Mavromatis K , Mikhailova N , Pati A , Brambilla E , Chen A , Palaniappan K , Land M , Hauser L , Chang YJ , Jeffries CD , Sikorski J , Spring S , Rohde M , Eichinger K , Huber H , Wirth R , Goker M , Detter JC , Woyke T , Bristow J , Eisen JA , Markowitz V , Hugenholtz P , Klenk HP , Kyrpides NC
Ref : Stand Genomic Sci , 3 :315 , 2010
Abstract : Methanothermus fervidus Stetter 1982 is the type strain of the genus Methanothermus. This hyperthermophilic genus is of a thought to be endemic in Icelandic hot springs. M. fervidus was not only the first characterized organism with a maximal growth temperature (97 degrees C) close to the boiling point of water, but also the first archaeon in which a detailed functional analysis of its histone protein was reported and the first one in which the function of 2,3-cyclodiphosphoglycerate in thermoadaptation was characterized. Strain V24S(T) is of interest because of its very low substrate ranges, it grows only on H(2) + CO(2). This is the first completed genome sequence of the family Methanothermaceae. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 1,243,342 bp long genome with its 1,311 protein-coding and 50 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
ESTHER : Anderson_2010_Stand.Genomic.Sci_3_315
PubMedSearch : Anderson_2010_Stand.Genomic.Sci_3_315
PubMedID: 21304736

Title : Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion - Martinez_2009_Proc.Natl.Acad.Sci.U.S.A_106_1954
Author(s) : Martinez D , Challacombe J , Morgenstern I , Hibbett D , Schmoll M , Kubicek CP , Ferreira P , Ruiz-Duenas FJ , Martinez AT , Kersten P , Hammel KE , Vanden Wymelenberg A , Gaskell J , Lindquist E , Sabat G , Bondurant SS , Larrondo LF , Canessa P , Vicuna R , Yadav J , Doddapaneni H , Subramanian V , Pisabarro AG , Lavin JL , Oguiza JA , Master E , Henrissat B , Coutinho PM , Harris P , Magnuson JK , Baker SE , Bruno K , Kenealy W , Hoegger PJ , Kues U , Ramaiya P , Lucas S , Salamov A , Shapiro H , Tu H , Chee CL , Misra M , Xie G , Teter S , Yaver D , James T , Mokrejs M , Pospisek M , Grigoriev IV , Brettin T , Rokhsar D , Berka R , Cullen D
Ref : Proc Natl Acad Sci U S A , 106 :1954 , 2009
Abstract : Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome, and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exocellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in medium containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative beta-1-4 endoglucanase were expressed at high levels relative to glucose-grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Also up-regulated during growth on cellulose medium were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H(2)O(2). These observations are consistent with a biodegradative role for Fenton chemistry in which Fe(II) and H(2)O(2) react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. Comparisons with the closely related white-rot fungus Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which the capacity for efficient depolymerization of lignin was lost.
ESTHER : Martinez_2009_Proc.Natl.Acad.Sci.U.S.A_106_1954
PubMedSearch : Martinez_2009_Proc.Natl.Acad.Sci.U.S.A_106_1954
PubMedID: 19193860
Gene_locus related to this paper: pospm-b8p1f3 , pospm-b8p2q7 , pospm-b8p4n0 , pospm-b8p4n9 , pospm-b8p5g9 , pospm-b8p5r9 , pospm-b8p6h2 , pospm-b8p7b1 , pospm-b8p7c4 , pospm-b8p8w7 , pospm-b8p9j1 , pospm-b8p164 , pospm-b8p280 , pospm-b8p423.1 , pospm-b8p423.2 , pospm-b8p858 , pospm-b8pam2 , pospm-b8pam5 , pospm-b8pb68 , pospm-b8pbm3 , pospm-b8pc54 , pospm-b8pc56 , pospm-b8pce4 , pospm-b8pd91 , pospm-b8pdk6 , pospm-b8ph32 , pospm-b8ph43 , pospm-b8phc9 , pospm-b8php7 , pospm-b8phy5 , pospm-b8pjg8 , pospm-b8pji9 , pospm-b8plr5 , pospm-b8pmk3 , pospm-b8pfg0 , pospm-b8pg35 , pospm-b8pa20.1 , pospm-b8pa20.2 , pospm-b8p4g8 , pospm-b8phn6

Title : Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina) - Martinez_2008_Nat.Biotechnol_26_553
Author(s) : Martinez D , Berka RM , Henrissat B , Saloheimo M , Arvas M , Baker SE , Chapman J , Chertkov O , Coutinho PM , Cullen D , Danchin EG , Grigoriev IV , Harris P , Jackson M , Kubicek CP , Han CS , Ho I , Larrondo LF , de Leon AL , Magnuson JK , Merino S , Misra M , Nelson B , Putnam N , Robbertse B , Salamov AA , Schmoll M , Terry A , Thayer N , Westerholm-Parvinen A , Schoch CL , Yao J , Barabote R , Nelson MA , Detter C , Bruce D , Kuske CR , Xie G , Richardson P , Rokhsar DS , Lucas SM , Rubin EM , Dunn-Coleman N , Ward M , Brettin TS
Ref : Nat Biotechnol , 26 :553 , 2008
Abstract : Trichoderma reesei is the main industrial source of cellulases and hemicellulases used to depolymerize biomass to simple sugars that are converted to chemical intermediates and biofuels, such as ethanol. We assembled 89 scaffolds (sets of ordered and oriented contigs) to generate 34 Mbp of nearly contiguous T. reesei genome sequence comprising 9,129 predicted gene models. Unexpectedly, considering the industrial utility and effectiveness of the carbohydrate-active enzymes of T. reesei, its genome encodes fewer cellulases and hemicellulases than any other sequenced fungus able to hydrolyze plant cell wall polysaccharides. Many T. reesei genes encoding carbohydrate-active enzymes are distributed nonrandomly in clusters that lie between regions of synteny with other Sordariomycetes. Numerous genes encoding biosynthetic pathways for secondary metabolites may promote survival of T. reesei in its competitive soil habitat, but genome analysis provided little mechanistic insight into its extraordinary capacity for protein secretion. Our analysis, coupled with the genome sequence data, provides a roadmap for constructing enhanced T. reesei strains for industrial applications such as biofuel production.
ESTHER : Martinez_2008_Nat.Biotechnol_26_553
PubMedSearch : Martinez_2008_Nat.Biotechnol_26_553
PubMedID: 18454138
Gene_locus related to this paper: hypjq-g0rh85 , hypjq-cip2 , hypjq-g0r9d1 , hypjq-g0r810 , hypjq-g0rbm4 , hypjq-g0rez4 , hypjq-g0rfr3 , hypjq-g0rg60 , hypjq-g0rij9 , hypjq-g0riu1 , hypjq-g0rl87 , hypjq-g0rlh4 , hypjq-g0rme5 , hypjq-g0rwy5 , hypje-axylest , hypje-q7z9m3 , hypjq-g0r6x2 , hypje-a0a024s1b8 , hypjr-a0a024s1s9 , hypjq-g0rxi5

Title : Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd - Challacombe_2007_J.Bacteriol_189_1890
Author(s) : Challacombe JF , Duncan AJ , Brettin TS , Bruce D , Chertkov O , Detter JC , Han CS , Misra M , Richardson P , Tapia R , Thayer N , Xie G , Inzana TJ
Ref : Journal of Bacteriology , 189 :1890 , 2007
Abstract : Haemophilus somnus can be either a commensal of bovine mucosal surfaces or an opportunistic pathogen. Pathogenic strains of H. somnus are a significant cause of systemic disease in cattle. We report the genome sequence of H. somnus 129Pt, a nonpathogenic commensal preputial isolate, and the results of a genome-wide comparative analysis of H. somnus 129Pt, Haemophilus influenzae Rd, and Haemophilus ducreyi 35000HP. We found unique genes in H. somnus 129Pt involved in lipooligosaccharide biosynthesis, carbohydrate uptake and metabolism, cation transport, amino acid metabolism, ubiquinone and menaquinone biosynthesis, cell surface adhesion, biosynthesis of cofactors, energy metabolism, and electron transport. There were also many genes in common among the three organisms. Our comparative analyses of H. somnus 129Pt, H. influenzae Rd, and H. ducreyi 35000HP revealed similarities and differences in the numbers and compositions of genes involved in metabolism, host colonization, and persistence. These results lay a foundation for research on the host specificities and niche preferences of these organisms. Future comparisons between H. somnus 129Pt and virulent strains will aid in the development of protective strategies and vaccines to protect cattle against H. somnus disease.
ESTHER : Challacombe_2007_J.Bacteriol_189_1890
PubMedSearch : Challacombe_2007_J.Bacteriol_189_1890
PubMedID: 17172329
Gene_locus related to this paper: haes1-q0i317 , haes1-q0i470 , haes2-b0utw7 , haes2-b0uvn0 , haes2-b0uwc2

Title : The complete genome sequence of Bacillus thuringiensis Al Hakam - Challacombe_2007_J.Bacteriol_189_3680
Author(s) : Challacombe JF , Altherr MR , Xie G , Bhotika SS , Brown N , Bruce D , Campbell CS , Campbell ML , Chen J , Chertkov O , Cleland C , Dimitrijevic M , Doggett NA , Fawcett JJ , Glavina T , Goodwin LA , Green LD , Han CS , Hill KK , Hitchcock P , Jackson PJ , Keim P , Kewalramani AR , Longmire J , Lucas S , Malfatti S , Martinez D , McMurry K , Meincke LJ , Misra M , Moseman BL , Mundt M , Munk AC , Okinaka RT , Parson-Quintana B , Reilly LP , Richardson P , Robinson DL , Saunders E , Tapia R , Tesmer JG , Thayer N , Thompson LS , Tice H , Ticknor LO , Wills PL , Gilna P , Brettin TS
Ref : Journal of Bacteriology , 189 :3680 , 2007
Abstract : Bacillus thuringiensis is an insect pathogen that is widely used as a biopesticide (E. Schnepf, N. Crickmore, J. Van Rie, D. Lereclus, J. Baum, J. Feitelson, D. R. Zeigler, and D. H. Dean, Microbiol. Mol. Biol. Rev. 62:775-806, 1998). Here we report the finished, annotated genome sequence of B. thuringiensis Al Hakam, which was collected in Iraq by the United Nations Special Commission (L. Radnedge, P. Agron, K. Hill, P. Jackson, L. Ticknor, P. Keim, and G. Andersen, Appl. Environ. Microbiol. 69:2755-2764, 2003).
ESTHER : Challacombe_2007_J.Bacteriol_189_3680
PubMedSearch : Challacombe_2007_J.Bacteriol_189_3680
PubMedID: 17337577
Gene_locus related to this paper: bacah-a0rcd1 , bacah-a0rer5 , bacah-a0rev7 , bacan-BA1019 , bacan-BA1242 , bacan-BA2392 , bacan-BA2607 , bacan-BA3343 , bacan-BA3863 , bacan-BA3877 , bacan-BA4324 , bacan-BA4338 , bacan-BA4577 , bacan-BA5009 , bacan-BA5110 , bacan-BA5136 , bacan-DHBF , bacc1-q73a27 , bacc1-q73c93 , bacce-BC0192 , bacce-BC1788 , bacce-BC1954 , bacce-BC2141 , bacce-BC2171 , bacce-BC4730 , bacce-BC4862 , bacce-BC5130 , bacce-PHAC , bacce-q72yu1 , baccr-pepx , bachk-q6hcl3 , bachk-q6hgn4 , bachk-q6hgp9 , bachk-q6hig3 , bachk-q6hit8

Title : Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii - Xie_2007_Appl.Environ.Microbiol_73_3536
Author(s) : Xie G , Bruce DC , Challacombe JF , Chertkov O , Detter JC , Gilna P , Han CS , Lucas S , Misra M , Myers GL , Richardson P , Tapia R , Thayer N , Thompson LS , Brettin TS , Henrissat B , Wilson DB , McBride MJ
Ref : Applied Environmental Microbiology , 73 :3536 , 2007
Abstract : The complete DNA sequence of the aerobic cellulolytic soil bacterium Cytophaga hutchinsonii, which belongs to the phylum Bacteroidetes, is presented. The genome consists of a single, circular, 4.43-Mb chromosome containing 3,790 open reading frames, 1,986 of which have been assigned a tentative function. Two of the most striking characteristics of C. hutchinsonii are its rapid gliding motility over surfaces and its contact-dependent digestion of crystalline cellulose. The mechanism of C. hutchinsonii motility is not known, but its genome contains homologs for each of the gld genes that are required for gliding of the distantly related bacteroidete Flavobacterium johnsoniae. Cytophaga-Flavobacterium gliding appears to be novel and does not involve well-studied motility organelles such as flagella or type IV pili. Many genes thought to encode proteins involved in cellulose utilization were identified. These include candidate endo-beta-1,4-glucanases and beta-glucosidases. Surprisingly, obvious homologs of known cellobiohydrolases were not detected. Since such enzymes are needed for efficient cellulose digestion by well-studied cellulolytic bacteria, C. hutchinsonii either has novel cellobiohydrolases or has an unusual method of cellulose utilization. Genes encoding proteins with cohesin domains, which are characteristic of cellulosomes, were absent, but many proteins predicted to be involved in polysaccharide utilization had putative D5 domains, which are thought to be involved in anchoring proteins to the cell surface.
ESTHER : Xie_2007_Appl.Environ.Microbiol_73_3536
PubMedSearch : Xie_2007_Appl.Environ.Microbiol_73_3536
PubMedID: 17400776
Gene_locus related to this paper: cyth3-q11pu3 , cyth3-q11rb1 , cyth3-q11sp5 , cyth3-q11sp6 , cyth3-q11ty5 , cyth3-q11vh6 , cyth3-q11vj5 , cyth3-q11w17 , cyth3-q11xy0

Title : Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis - Han_2006_J.Bacteriol_188_3382
Author(s) : Han CS , Xie G , Challacombe JF , Altherr MR , Bhotika SS , Brown N , Bruce D , Campbell CS , Campbell ML , Chen J , Chertkov O , Cleland C , Dimitrijevic M , Doggett NA , Fawcett JJ , Glavina T , Goodwin LA , Green LD , Hill KK , Hitchcock P , Jackson PJ , Keim P , Kewalramani AR , Longmire J , Lucas S , Malfatti S , McMurry K , Meincke LJ , Misra M , Moseman BL , Mundt M , Munk AC , Okinaka RT , Parson-Quintana B , Reilly LP , Richardson P , Robinson DL , Rubin E , Saunders E , Tapia R , Tesmer JG , Thayer N , Thompson LS , Tice H , Ticknor LO , Wills PL , Brettin TS , Gilna P
Ref : Journal of Bacteriology , 188 :3382 , 2006
Abstract : Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of B. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.
ESTHER : Han_2006_J.Bacteriol_188_3382
PubMedSearch : Han_2006_J.Bacteriol_188_3382
PubMedID: 16621833
Gene_locus related to this paper: bacan-BA0954 , bacan-BA2607 , bacce-BC0968 , bacce-BC3133 , bacce-BC5130 , bacce-c2mr40 , baccz-q63gk2