bacsu-lip

 
Bacillus subtilis lipase (lipA) (EC 3.1.1.3) BSLA (triacylglycerol lipase)

Comment
One of the smallest lipase. It lacks a lid covering the substrate cleft.the active Ser in AXSXG pentapeptide.Prefers substrates with C8 fatty acid chains It shows remarkable stability at low pH. Also called LIPA or ESTA as it is more an esterase than a lipase. Bacillus subtilis (subsp. spizizenii (ATCC 23059 / NRRL B-14472 / W23; ATCC 6633); BSn5; subsp. natto BEST195; 168)


Relationship
Family Lipase_2
Block X
Bacillus subtilis position in NCBI Life Tree :
N link to NCBI taxonomic web page and E link to ESTHER gene locus found in this strain.
> cellular organisms: N E > Bacteria: N E > Terrabacteria group: N E > Firmicutes: N E > Bacilli: N E > Bacillales: N E > Bacillaceae: N E > Bacillus: N E > Bacillus subtilis group: N E > Bacillus subtilis: N E
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data


Molecular evidence
Database
No mutation
21 structures (e.g. : 5CT9, 5CT6, 5CT8... more)
No kinetic

Substrate: Ketoprofen-Ethyl-Ester ,
Inhibitor: IPG-Phosphonate ,
>3 Genbank links 1 more: M74010, AB000617, Z99105
>3 UniProt links 3 more: P37957, A5HLW9, E0U0Y0
>3 Structure links 18 more: 5CT9, 5CT6, 5CT8
>3 UniProtTrembl links 3 more: P37957, A5HLW9, E0U0Y0
>3 Interpro links 3 more: P37957, A5HLW9, E0U0Y0
>3 Prodom links 3 more: P37957, A5HLW9, E0U0Y0
>3 Pfam links 3 more: P37957, A5HLW9, E0U0Y0
>3 PIRSF links 3 more: P37957, A5HLW9, E0U0Y0
>3 SUPERFAM links 3 more: P37957, A5HLW9, E0U0Y0
>3 QuickSwissBlast links 3 more: P37957, A5HLW9, E0U0Y0
 
Sequence
Graphical view for this peptide sequence: bacsu-lip
Colored MSA for Lipase_2 (raw)
MKFVKRRIIALVTILMLSVTSLFALQPSAKAAEHNPVVMVHGIGGASFNF
AGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAK
KVDIVAHSMGGANTLYYIKNLDGGNKVANVVTLGGANRLTTGKALPGTDP
NQKILYTSIYSSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLI
KEGLNGGGQNTN
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA

MKFVKRRIIALVTILMLSVTSLFALQPSAKAAEHNPVVMVHGIGGASFNF
AGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAK
KVDIVAHSMGGANTLYYIKNLDGGNKVANVVTLGGANRLTTGKALPGTDP
NQKILYTSIYSSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLI
KEGLNGGGQNTN

no DNA




References
24 more
    Title: Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents
    Cui H, Vedder M, Schwaneberg U, Davari MD
    Ref: Methods Mol Biol, 2397:179, 2022 : PubMed

            

    Title: Effect of Organic Solvents on the Structure and Activity of a Minimal Lipase
    Ingenbosch KN, Vieyto-Nunez JC, Ruiz-Blanco YB, Mayer C, Hoffmann-Jacobsen K, Sanchez-Garcia E
    Ref: J Org Chem, :, 2021 : PubMed

            

    Title: Rosetta-Enabled Structural Prediction of Permissive Loop Insertion Sites in Proteins
    Plaks JG, Brewer JA, Jacobsen NK, McKenna M, Uzarski JR, Lawton TJ, Filocamo SF, Kaar JL
    Ref: Biochemistry, 59:3993, 2020 : PubMed

            


Other Papers


Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
For technical information about these pages see:
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