GTSAGmotif

Relationship

Family: GTSAGmotif

Block: H

Parent Family: No family

Comment

Sequences of lipases from metagenomic library were grouped in a new subset of Alphabeta hydrolase fold-3 (Arpigny_Jaeger Family_IV). Most sequences have a G(TDC)SA(G)G motif encompassing the active site serine.

Database

Interpro : IPR013094 Alpha\/beta hydrolase fold-3

PIRSF : No PIRSF

Pdoc : No Pdoc

Pfam : PF07859 Abhydrolase_3

Prints : No Print

EC Number : No EC Number

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
6ZL7 Crystal structure of C173S mutation in the PGML2 esterase from permafrost metagenomic library 9bact-a0a142j6i6
6QLA uncultured bacterium esterase PMGL2 (point mutant 1) from a permafrost metagenomic library. 9bact-a0a142j6i6
6QIN uncultured bacterium esterase PMGL2 from a permafrost metagenomic library. 9bact-a0a142j6i6
4Q3O Crystal structure of MGS-MT1, an alpha\/beta hydrolase enzyme from a Lake Matapan deep-sea metagenome library 9alte-MGS-MT1
4Q05 Crystal structure of an esterase E25 9bact-E25

Gene_locus Name Species
9bact-a0a142j6i6 uncultured bacterium esterase PMGL2 with GCSAG motif from a permafrost metagenomic library. uncultured bacterium
9sphn-AUD08548 Erythrobacter seohaensis strain SW135 esterase (e69) gene from tidal flat sediment of Yellow Sea Erythrobacter seohaensis
9alte-MGS-MT1 Alteromonas australica MGS-MT1,EH23 an alpha\/beta hydrolase from a Lake Matapan deep-sea metagenome library Alteromonas australica
frasn-a8leg3 Frankia sp. (strain EAN1pec) Alpha\/beta hydrolase fold-3 domain protein Frankia sp.
burs3-q396l8 Burkholderia lata esterase\/lipase\/thioesterase Burkholderia sp.
synsp-ester Synechocystis sp esterase Synechocystis sp.
xanca-XCC0080 Xanthomonas campestris (pv. campestris) esterase Xanthomonas campestris
xylfa-XF1743 Xylella fastidiosa, gene XF1743 Esterase Xylella fastidiosa
9bact-E25 Uncultured bacteria South China sea metagenomic novel esterase uncultured bacteria
9bact-b8y553 uncultured bacterium Lipolytic enzyme uncultured bacterium
9bact-b1pz92 uncultured bacterium Esterase uncultured bacterium
9bact-d8v1j5 uncultured bacterium Esterase uncultured bacterium
9gamm-a0ya83 marine gamma proteobacterium HTCC2143 Esterase marine gamma proteobacterium HTCC2143
9bact-d8v1j4 uncultured bacterium Esterase uncultured bacterium
altss-f5z698 Alteromonas sp. (strain SN2) Esterase Alteromonas sp.
vibna-s6lg09 Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 Uncharacterized protein Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759
vibph-w3zhu2 Vibrio parahaemolyticus 50 Alpha\/beta hydrolase fold family protein Vibrio parahaemolyticus 50
9delt-g1v5p4 Bilophila sp. 4_1_30 Uncharacterized protein Bilophila sp. 4_1_30
9bact-d8v1j3 uncultured bacterium Esterase uncultured bacterium
terrk-i3ze47 Terriglobus roseus (strain DSM 18391 \/ NRRL B-41598 \/ KBS 63) Esterase\/lipase Terriglobus roseus
altme-k0crz6 Alteromonas macleodii (strain English Channel 673) Esterase Alteromonas macleodii
9rhiz-i9lge7 Methylobacterium sp. GXF4 Alpha\/beta hydrolase domain-containing protein Methylobacterium sp. GXF4
metrj-b1lym6 Methylobacterium radiotolerans (strain ATCC 27329 \/ DSM 1819 \/ JC 2831) Alpha\/beta hydrolase fold-3 domain protein Methylobacterium radiotolerans
mets4-b0ud71 Methylobacterium sp. (strain 4-46) Alpha\/beta hydrolase fold-3 domain protein Methylobacterium sp.
altma-j9ycg2 Alteromonas macleodii ATCC 27126 Esterase Alteromonas macleodii ATCC 27126
agaal-r9ptw5 Agarivorans albus MKT 106 Esterase Agarivorans albus MKT 106
9brad-m4z0c0 Bradyrhizobium oligotrophicum S58 Alpha\/beta hydrolase fold-3 domain protein Bradyrhizobium oligotrophicum S58
9brad-h0t2z1 Bradyrhizobium sp. STM 3809 Alpha\/beta hydrolase fold-3 domain protein Bradyrhizobium sp. STM 3809
9brad-u1hd63 Bradyrhizobium sp. DFCI-1 Cytochrome c oxidase cbb3-type subunit IV Bradyrhizobium sp. DFCI-1
altmb-k0ej12 Alteromonas macleodii (strain Balearic Sea AD45) Esterase Alteromonas macleodii
9brad-h0trw8 Bradyrhizobium sp. STM 3843 Alpha\/beta hydrolase fold-3 domain protein Bradyrhizobium sp. STM 3843
shehh-b0tmq3 Shewanella halifaxensis (strain HAW-EB4) Alpha\/beta hydrolase fold-3 domain protein Shewanella halifaxensis
9brad-j3hvp0 Bradyrhizobium sp. YR681 Esterase\/lipase Bradyrhizobium sp. YR681
altms-k0d8p8 Alteromonas macleodii (strain Black Sea 11) Esterase Alteromonas macleodii
mets4-b0und9 Methylobacterium sp. (strain 4-46) Alpha\/beta hydrolase fold-3 domain protein Methylobacterium sp.
altma-s5cfn7 Alteromonas macleodii EH119 Esterase Alteromonas macleodii
9bact-d2kla1 uncultured marine bacterium Lipolytic enzyme uncultured marine bacterium
9rhiz-m7ymz2 Methylobacterium mesophilicum SR1.6\/6 Alpha\/beta hydrolase domain-containing protein Methylobacterium mesophilicum SR1.6\/6
9bact-d7nj22 uncultured bacterium alkaline-stable lipase uncultured bacterium
vibsl-b7vqj9 Vibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32)) Putative esterase Vibrio splendidus
9sphn-i9c598 Novosphingobium sp. Rr 2-17 Esterase Novosphingobium sp. Rr 2-17
9burk-r7kjn3 Sutterella sp. CAG:521 Esterase Sutterella sp. CAG:521
9gamm-e8lk20 Succinatimonas hippei YIT 12066 Hydrolase, alpha\/beta domain protein Succinatimonas hippei YIT 12066
serp5-a8g8q2 Serratia proteamaculans (strain 568) Alpha\/beta hydrolase fold-3 domain protein Serratia proteamaculans
hypsm-f8jbt5 Hyphomicrobium sp. (strain MC1) Alpha\/beta hydrolase fold-3 domain protein Hyphomicrobium sp.
metno-b8iks9 Methylobacterium nodulans (strain ORS2060 \/ LMG 21967) Alpha\/beta hydrolase fold-3 domain protein Methylobacterium nodulans
frasu-e3j816 Frankia sp. (strain EuI1c) Esterase\/lipase\/thioesterase Frankia sp.
frasu-e3ivg3 Frankia sp. (strain EuI1c) Esterase\/lipase\/thioesterase Frankia sp.
9sphn-w0a8l5 Sphingomonas sanxanigenens DSM 19645 = NX02 Uncharacterized protein Sphingomonas sanxanigenens DSM 19645 = NX02
rhitr-l0lr17 Rhizobium tropici CIAT 899 Alpha\/beta hydrolase fold-3 catalytic domain-containing protein Rhizobium tropici CIAT 899
9rhiz-j3htx9 Phyllobacterium sp. YR531 Esterase\/lipase Phyllobacterium sp. YR531
rhird-w1lea0 Agrobacterium radiobacter DSM 30147 Esterase Agrobacterium radiobacter DSM 30147
frasn-a8lfl3 Frankia sp. (strain EAN1pec) Alpha\/beta hydrolase fold-3 domain protein Frankia sp.
9prot-w6ikt1 Granulibacter bethesdensis CGDNIH2 Esterase Granulibacter bethesdensis CGDNIH2
grabc-q0br83 Granulibacter bethesdensis (strain ATCC BAA-1260 \/ CGDNIH1) Esterase Granulibacter bethesdensis
rhird-h0hik8 Agrobacterium tumefaciens 5A Esterase Agrobacterium tumefaciens 5A
frasn-a8leg7 Frankia sp. (strain EAN1pec) Alpha\/beta hydrolase fold-3 domain protein Frankia sp.
grabc-q0br68 Granulibacter bethesdensis Esterase Granulibacter bethesdensis
9rhiz-u4qhk6 Rhizobium sp. IRBG74 Esterase Rhizobium sp. IRBG74
agrsh-f0lfk1 Agrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3)) Esterase Agrobacterium sp.
9burk-g7hau7 Burkholderia cenocepacia H111 Esterase\/lipase, sll0644 homolog Burkholderia cenocepacia H111
frasu-e3j831 Frankia sp. (strain EuI1c) Esterase\/lipase\/thioesterase family protein Frankia sp.
burcj-b4ek59 Burkholderia cenocepacia (Burkholderia cepacia) Putative esterase Burkholderia cenocepacia
9acto-d3cti7 Frankia sp. EUN1f Alpha\/beta hydrolase fold-3 domain protein Frankia sp. EUN1f
9sphn-f3wtf3 Sphingomonas sp. S17 Alpha\/beta hydrolase fold family protein Sphingomonas sp. S17
frasu-e3j9a0 Frankia sp. (strain EuI1c) Esterase Frankia sp.
psefl-v7ecg4 Pseudomonas fluorescens\; Pseudomonas sp., Esterase\/lipase Pseudomonas fluorescens BBc6R8
9entr-j0w699 Enterobacter sp. Ag1 Esterase Enterobacter sp. Ag1
9sphn-t0gfp3 Sphingobium quisquiliarum P25 Uncharacterized protein Sphingobium quisquiliarum P25
agrrk-b9jls9 Agrobacterium radiobacter (strain K84 \/ ATCC BAA-868) Esterase\/lipase\/thioesterase family protein Agrobacterium radiobacter
9sphn-u3a0m8 Novosphingobium tardaugens NBRC 16725 Uncharacterized protein Novosphingobium tardaugens NBRC 16725
9sphn-u2y5p9 Novosphingobium tardaugens NBRC 16725 Putative esterase Novosphingobium tardaugens NBRC 16725
frasu-e3j9a7 Frankia sp. (strain EuI1c) Esterase\/lipase\/thioesterase Frankia sp.
desao-e6vth1 Desulfovibrio aespoeensis (strain ATCC 700646 \/ DSM 10631 \/ Aspo-2) Alpha\/beta hydrolase domain-containing protein Desulfovibrio aespoeensis
9bact-d5mt93 uncultured bacterium Esterase uncultured bacterium
9bact-a3rgw9 uncultured bacterium Putative esterase uncultured bacterium
9bact-h6bdx2 uncultured bacterium Lipolytic enzyme uncultured bacterium
9sphn-u2yih8 Novosphingobium tardaugens NBRC 16725 Putative hydrolase Novosphingobium tardaugens NBRC 16725
novad-a4xew4 Novosphingobium aromaticivorans (strain DSM 12444 \/ F199) Alpha\/beta hydrolase fold-3 domain protein Novosphingobium aromaticivorans
korve-q1iuf6 Koribacter versatilis (strain Ellin345) Alpha\/beta hydrolase Koribacter versatilis
terrk-i3zbs2 Terriglobus roseus (strain DSM 18391 \/ NRRL B-41598 \/ KBS 63) Esterase\/lipase Terriglobus roseus
caust-d5vhk7 Caulobacter segnis (strain ATCC 21756 \/ DSM 7131 \/ JCM 7823 \/ NBR 15250 \/ LMG 17158 \/ TK0059) (Mycoplana segnis) Alpha\/beta hydrolase fold-3 domain protein Caulobacter segnis
cauce-r0ekv3 Caulobacter crescentus OR37 Esterase\/lipase Caulobacter crescentus OR37
9sphn-j2lbm8 Sphingobium sp. AP49 Esterase\/lipase Sphingobium sp. AP49
sphya-k9d6e0 Sphingobium yanoikuyae ATCC 51230 Uncharacterized protein Sphingobium yanoikuyae ATCC 51230
fusox-x0m2c2 Fusarium oxysporum f. sp. vasinfectum 25433 Uncharacterized protein Fusarium oxysporum f. sp. vasinfectum 25433
9bact-b8y558 uncultured bacterium Lipolytic enzyme uncultured bacterium
9xant-f0bgj0 Xanthomonas vesicatoria ATCC 35937 Esterase\/lipase Xanthomonas vesicatoria ATCC 35937
9bact-b8y563 uncultured bacterium Lipolytic enzyme uncultured bacterium
9zzzz-e6q0e6 mine drainage metagenome Alpha\/beta hydrolase mine drainage metagenome
hypvg-g9nbv2 Hypocrea virens (strain Gv29-8 \/ FGSC 10586) (Gliocladium virens) (Trichoderma virens) Uncharacterized protein Hypocrea virens
9prot-g1y2y6 Azospirillum amazonense Y2 Alpha\/beta hydrolase fold domain protein Azospirillum amazonense Y2
fusox-w9hiy6 Fusarium oxysporum Uncharacterized protein Fusarium oxysporum FOSC 3-a
9sphn-n1msk9 Sphingobium japonicum BiD32 Esterase Sphingobium japonicum BiD32
9xant-v7zi90 Xanthomonas hortorum pv. carotae str. M081 Esterase Xanthomonas hortorum pv. carotae str. M081
gibf5-s0ekc4 Gibberella fujikuroi (strain CBS 195.34 \/ IMI 58289 \/ NRRL A-6831) (Bakanae and foot rot disease fungus) (Fusarium fujikuroi) Uncharacterized protein Gibberella fujikuroi
acic5-c1f891 Acidobacterium capsulatum (strain ATCC 51196 \/ DSM 11244 \/ JCM 7670) Esterase Acidobacterium capsulatum
fusox-w9nxk0 Fusarium oxysporum f. sp. pisi HDV247 Uncharacterized protein Fusarium oxysporum f. sp. pisi HDV247
asptn-q0cg08 Aspergillus terreus (strain NIH 2624 \/ FGSC A1156) Predicted protein Aspergillus terreus
9sphn-w0a9g1 Sphingomonas sanxanigenens DSM 19645 = NX02 Uncharacterized protein Sphingomonas sanxanigenens DSM 19645 = NX02
9caul-v4pmj2 Asticcacaulis sp. AC460 Uncharacterized protein Asticcacaulis sp. AC460
9xant-f0cdj1 Xanthomonas gardneri ATCC 19865 Esterase\/lipase Xanthomonas gardneri ATCC 19865
9xant-w4syz3 Xanthomonas arboricola pv. pruni Exported acetyl esterase Xanthomonas arboricola pv. pruni MAFF 301427
9sphn-f3x3b1 Sphingomonas sp. S17 Alpha\/beta hydrolase fold family protein Sphingomonas sp. S17
fusox-x0h323 Fusarium oxysporum f. sp. conglutinans race 2 54008 Uncharacterized protein Fusarium oxysporum f. sp. conglutinans race 2 54008
9sphn-j2gm31 Novosphingobium sp. AP12 Esterase\/lipase Novosphingobium sp. AP12
shesh-a8ft15 Shewanella sediminis (strain HAW-EB3) Esterase\/lipase-like protein Shewanella sediminis
fusox-x0k2p3 Fusarium oxysporum f. sp. cubense tropical race 4 54006 Uncharacterized protein Fusarium oxysporum f. sp. cubense tropical race 4 54006
9delt-w4lci7 Candidatus Entotheonella sp. TSY1 Uncharacterized protein Candidatus Entotheonella sp. TSY1
gibm7-w7n497 Gibberella moniliformis (strain M3125 \/ FGSC 7600) (Maize ear and stalk rot fungus) (Fusarium verticillioides) Uncharacterized protein Gibberella moniliformis
solue-q026h7 Solibacter usitatus (strain Ellin6076) Alpha\/beta hydrolase Solibacter usitatus
9sphn-j8sju4 Sphingomonas sp. LH128 Alpha\/beta hydrolase domain-containing protein Sphingomonas sp. LH128

Gene_locus_frgt Name Species
9burk-b1t1g6 Burkholderia ambifaria MEX-5 Alpha\/beta hydrolase fold-3 domain protein Burkholderia ambifaria MEX-5
9rhiz-k2qkb8 Agrobacterium albertimagni AOL15 Esterase\/lipase\/thioesterase family protein Agrobacterium albertimagni AOL15
9zzzz-x0t7r8 marine sediment metagenome Marine sediment metagenome DNA, contig: S01H1_L12205 marine sediment metagenome
frant-i2b041 Francisella noatunensis subsp. orientalis (strain Toba 04) Alpha\/beta hydrolase domain-containing protein Francisella noatunensis subsp. orientalis
9sphn-g2iq94 Sphingobium sp. SYK-6 Putative esterase Sphingobium sp. SYK-6
9bact-h6bdw2 uncultured bacterium Lipolytic enzyme uncultured bacterium
mycne-v5xdk1 Mycobacterium neoaurum VKM Ac-1815D Alpha\/beta hydrolase Mycobacterium neoaurum VKM Ac-1815D
9rhiz-w6s8s5 Rhizobium sp. LPU83 Esterase\/lipase\/thioesterase family protein Rhizobium sp. LPU83
9mico-k1e0f4 Janibacter hoylei PVAS-1 Alpha\/beta hydrolase domain-containing protein Janibacter hoylei PVAS-1

Substrate Chemical Nomenclature Proteins
Paranitrophenyl-hexanoate (4-nitrophenyl) hexanoate erylo-E53    9bact-a0a2s1gux0    9flao-a0a330mq60    9flao-a0a0c1f4u8    myctu-Rv1430    geost-est30    geost-est50
Paranitrophenylacetate (4-nitrophenyl) acetate arath-AT5G62180    9psed-q0mrg5    burgl-EstC    9bace-b3cet1    9bace-b3c9d0.2    emeni-faec    9acto-h1qtl0    strco-SCO2123    human-NOTUM    pseae-PA3859    pyrbr-Q9Y7G8    9baci-EstD9    9rhiz-g9i933    malgo-a8puy1    human-CIB    9actn-EstGS1
Glucose-pentaacetate [(2R,3R,4S,5R,6R)-3,4,5,6-tetraacetyloxyoxan-2-yl]methyl acetate canru-3lipa    9alte-MGS-MT1    9bact-a0a1s5qkg0    9zzzz-a0a0g3fl25    9bact-a0a0b5kc16    thema-TM0077
Paranitrophenylbutyrate (4-nitrophenyl) butanoate bactc-lipas    9acto-f7ix06    artox-phhyd    burgl-EstC    bovin-balip    rabit-1cxes    psesp-OPH    9bact-H6BDX1    9bact-a0a2s1gux0    human-NOTUM    9delt-EstD11    9pezi-s4vch4    aspfu-q4x1n0    crysp-Q874E9    fusso-cutas    pyrbr-Q9Y7G8    copci-b9u443    strsw-c9zcr8    humin-cut    human-DAGLA    9baci-EstD9    9rhiz-g9i933    canar-LipB    bacsu-pnbae    thefu-q6a0i4    geost-est30    geost-est50    pyrab-PAB1050
Paranitrophenyl-octanoate (4-nitrophenyl) octanoate 9firm-a0a0a0ymq9    9bact-Q0GMU2    psesp-OPH    human-NOTUM    pseae-PA2949    crysp-Q874E9    emeni-q5b2c1    fusso-cutas    thite-g2rae6    9flao-a0a330mq60    9flao-a0a0c1f4u8    malgo-a8puy1    9bact-6EB3    partm-GthFAE    9bact-a0a2h5z9r5    9actn-j7k890    9actn-j7kdc6    fusox-a0a1d3s5h0
Paranitrophenyl-decanoate (4-nitrophenyl) decanoate

Inhibitor Chemical Nomenclature Proteins
PMSF Phenylmethylsulfonyl fluoride theet-q3ch51    emeni-q5avy9    human-ABHD10    human-PREPL    myctu-Rv1430    psefl-cxest    9bact-E40    thefu-q6a0i4    ecoli-Aes    orysj-Q10QA5    bac25-mglp    9bact-Q0GMU2    bacsu-FENB    psepu-clcd1    thema-TM0077    bacsu-RsbQ    ecoli-bioh    drome-CG9542    human-FAP    tubmm-TmEst2    human-NOTUM    cutac-e6dae5    9gamm-urc15994    9baci-EstD9    geost-est30    pholl-AntI

References (5)

Title : Crystal structure of PMGL2 esterase from the hormone-sensitive lipase family with GCSAG motif around the catalytic serine - Boyko_2020_PLoS.One_15_e0226838
Author(s) : Boyko KM , Kryukova MV , Petrovskaya LE , Nikolaeva AY , Korzhenevsky DA , Novototskaya-Vlasova KA , Rivkina EM , Dolgikh DA , Kirpichnikov MP , Popov VO
Ref : PLoS ONE , 15 :e0226838 , 2020
Abstract :
PubMedSearch : Boyko_2020_PLoS.One_15_e0226838
PubMedID: 31990908
Gene_locus related to this paper: 9bact-a0a142j6i6

Title : Expression and characterization of a new esterase with GCSAG motif from a permafrost metagenomic library - Petrovskaya_2016_FEMS.Microbiol.Ecol_92_
Author(s) : Petrovskaya LE , Novototskaya-Vlasova KA , Spirina EV , Durdenko EV , Lomakina GY , Zavialova MG , Nikolaev EN , Rivkina EM
Ref : FEMS Microbiol Ecol , 92 : , 2016
Abstract :
PubMedSearch : Petrovskaya_2016_FEMS.Microbiol.Ecol_92_
PubMedID: 26929439
Gene_locus related to this paper: 9bact-a0a142j6i6

Title : Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats - Alcaide_2015_Environ.Microbiol_17_332
Author(s) : Alcaide M , Stogios PJ , Lafraya A , Tchigvintsev A , Flick R , Bargiela R , Chernikova TN , Reva ON , Hai T , Leggewie CC , Katzke N , La Cono V , Matesanz R , Jebbar M , Jaeger KE , Yakimov MM , Yakunin AF , Golyshin PN , Golyshina OV , Savchenko A , Ferrer M
Ref : Environ Microbiol , 17 :332 , 2015
Abstract :
PubMedSearch : Alcaide_2015_Environ.Microbiol_17_332
PubMedID: 25330254
Gene_locus related to this paper: 9alte-MGS-MT1 , 9bact-MGS-M1 , 9bact-MGS-M2 , 9bact-a0a0b5kns5

Title : Structural Basis for Dimerization and Catalysis of a Novel Esterase from the GTSAG Motif Subfamily of the Bacterial Hormone-sensitive Lipase Family - Li_2014_J.Biol.Chem_289_19031
Author(s) : Li PY , Ji P , Li CY , Zhang Y , Wang GL , Zhang XY , Xie BB , Qin QL , Chen XL , Zhou BC , Zhang YZ
Ref : Journal of Biological Chemistry , 289 :19031 , 2014
Abstract :
PubMedSearch : Li_2014_J.Biol.Chem_289_19031
PubMedID: 24867954
Gene_locus related to this paper: 9bact-E25

Title : Identification of a new subfamily of salt-tolerant esterases from a metagenomic library of tidal flat sediment - Jeon_2012_Appl.Microbiol.Biotechnol_93_623
Author(s) : Jeon JH , Lee HS , Kim JT , Kim SJ , Choi SH , Kang SG , Lee JH
Ref : Applied Microbiology & Biotechnology , 93 :623 , 2012
Abstract :
PubMedSearch : Jeon_2012_Appl.Microbiol.Biotechnol_93_623
PubMedID: 21720822
Gene_locus related to this paper: 9bact-E40 , 9bact-E25 , 9bact-d8v1j5 , 9bact-d8v1j4 , 9bact-d8v1j3