GTSAGmotif
Relationship
Comment
Sequences of lipases from metagenomic library were grouped in a new subset of Alphabeta hydrolase fold-3 (Arpigny_Jaeger Family_IV). Most sequences have a G(TDC)SA(G)G motif encompassing the active site serine.
Database
Interpro : IPR013094 Alpha\/beta hydrolase fold-3
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF07859 Abhydrolase_3
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 4Q05 | Crystal structure of an esterase E25 | 9bact-E25 |
| 4Q3O | Crystal structure of MGS-MT1, an alpha\/beta hydrolase enzyme from a Lake Matapan deep-sea metagenome library | 9alte-MGS-MT1 |
| 6QIN | uncultured bacterium esterase PMGL2 from a permafrost metagenomic library. | 9bact-a0a142j6i6 |
| 6QLA | uncultured bacterium esterase PMGL2 (point mutant 1) from a permafrost metagenomic library. | 9bact-a0a142j6i6 |
| 6ZL7 | Crystal structure of C173S mutation in the PGML2 esterase from permafrost metagenomic library | 9bact-a0a142j6i6 |
| Gene_locus | Name | Species |
|---|---|---|
| 9acto-d3cti7 | Frankia sp. EUN1f Alpha\/beta hydrolase fold-3 domain protein | Frankia sp. EUN1f |
| 9alte-MGS-MT1 |  Alteromonas australica MGS-MT1,EH23 an alpha\/beta hydrolase from a Lake Matapan deep-sea metagenome library |
Alteromonas australica |
| 9bact-E25 | Uncultured bacteria South China sea metagenomic novel esterase |
uncultured bacteria |
| 9bact-a0a142j6i6 | uncultured bacterium esterase PMGL2 with GCSAG motif from a permafrost metagenomic library. |
uncultured bacterium |
| 9bact-a3rgw9 | uncultured bacterium Putative esterase | uncultured bacterium |
| 9bact-b1pz92 | uncultured bacterium Esterase | uncultured bacterium |
| 9bact-b8y553 | uncultured bacterium Lipolytic enzyme | uncultured bacterium |
| 9bact-b8y558 | uncultured bacterium Lipolytic enzyme | uncultured bacterium |
| 9bact-b8y563 | uncultured bacterium Lipolytic enzyme | uncultured bacterium |
| 9bact-d2kla1 | uncultured marine bacterium Lipolytic enzyme | uncultured marine bacterium |
| 9bact-d5mt93 | uncultured bacterium Esterase | uncultured bacterium |
| 9bact-d7nj22 | uncultured bacterium alkaline-stable lipase | uncultured bacterium |
| 9bact-d8v1j3 | uncultured bacterium Esterase | uncultured bacterium |
| 9bact-d8v1j4 | uncultured bacterium Esterase | uncultured bacterium |
| 9bact-d8v1j5 | uncultured bacterium Esterase | uncultured bacterium |
| 9bact-h6bdx2 | uncultured bacterium Lipolytic enzyme | uncultured bacterium |
| 9brad-h0t2z1 | Bradyrhizobium sp. STM 3809 Alpha\/beta hydrolase fold-3 domain protein | Bradyrhizobium sp. STM 3809 |
| 9brad-h0trw8 | Bradyrhizobium sp. STM 3843 Alpha\/beta hydrolase fold-3 domain protein | Bradyrhizobium sp. STM 3843 |
| 9brad-j3hvp0 | Bradyrhizobium sp. YR681 Esterase\/lipase | Bradyrhizobium sp. YR681 |
| 9brad-m4z0c0 | Bradyrhizobium oligotrophicum S58 Alpha\/beta hydrolase fold-3 domain protein | Bradyrhizobium oligotrophicum S58 |
| 9brad-u1hd63 | Bradyrhizobium sp. DFCI-1 Cytochrome c oxidase cbb3-type subunit IV | Bradyrhizobium sp. DFCI-1 |
| 9burk-g7hau7 | Burkholderia cenocepacia H111 Esterase\/lipase, sll0644 homolog | Burkholderia cenocepacia H111 |
| 9burk-r7kjn3 | Sutterella sp. CAG:521 Esterase | Sutterella sp. CAG:521 |
| 9caul-v4pmj2 | Asticcacaulis sp. AC460 Uncharacterized protein | Asticcacaulis sp. AC460 |
| 9delt-g1v5p4 | Bilophila sp. 4_1_30 Uncharacterized protein | Bilophila sp. 4_1_30 |
| 9delt-w4lci7 | Candidatus Entotheonella sp. TSY1 Uncharacterized protein | Candidatus Entotheonella sp. TSY1 |
| 9entr-j0w699 | Enterobacter sp. Ag1 Esterase | Enterobacter sp. Ag1 |
| 9gamm-a0ya83 | marine gamma proteobacterium HTCC2143 Esterase | marine gamma proteobacterium HTCC2143 |
| 9gamm-e8lk20 | Succinatimonas hippei YIT 12066 Hydrolase, alpha\/beta domain protein | Succinatimonas hippei YIT 12066 |
| 9prot-g1y2y6 | Azospirillum amazonense Y2 Alpha\/beta hydrolase fold domain protein | Azospirillum amazonense Y2 |
| 9prot-w6ikt1 | Granulibacter bethesdensis CGDNIH2 Esterase | Granulibacter bethesdensis CGDNIH2 |
| 9rhiz-i9lge7 | Methylobacterium sp. GXF4 Alpha\/beta hydrolase domain-containing protein | Methylobacterium sp. GXF4 |
| 9rhiz-j3htx9 | Phyllobacterium sp. YR531 Esterase\/lipase | Phyllobacterium sp. YR531 |
| 9rhiz-m7ymz2 | Methylobacterium mesophilicum SR1.6\/6 Alpha\/beta hydrolase domain-containing protein | Methylobacterium mesophilicum SR1.6\/6 |
| 9rhiz-u4qhk6 | Rhizobium sp. IRBG74 Esterase | Rhizobium sp. IRBG74 |
| 9sphn-AUD08548 | Erythrobacter seohaensis strain SW135 esterase (e69) gene from tidal flat sediment of Yellow Sea | Erythrobacter seohaensis |
| 9sphn-f3wtf3 | Sphingomonas sp. S17 Alpha\/beta hydrolase fold family protein | Sphingomonas sp. S17 |
| 9sphn-f3x3b1 | Sphingomonas sp. S17 Alpha\/beta hydrolase fold family protein | Sphingomonas sp. S17 |
| 9sphn-i9c598 | Novosphingobium sp. Rr 2-17 Esterase | Novosphingobium sp. Rr 2-17 |
| 9sphn-j2gm31 | Novosphingobium sp. AP12 Esterase\/lipase | Novosphingobium sp. AP12 |
| 9sphn-j2lbm8 | Sphingobium sp. AP49 Esterase\/lipase | Sphingobium sp. AP49 |
| 9sphn-j8sju4 | Sphingomonas sp. LH128 Alpha\/beta hydrolase domain-containing protein | Sphingomonas sp. LH128 |
| 9sphn-n1msk9 | Sphingobium japonicum BiD32 Esterase | Sphingobium japonicum BiD32 |
| 9sphn-t0gfp3 | Sphingobium quisquiliarum P25 Uncharacterized protein | Sphingobium quisquiliarum P25 |
| 9sphn-u2y5p9 | Novosphingobium tardaugens NBRC 16725 Putative esterase | Novosphingobium tardaugens NBRC 16725 |
| 9sphn-u2yih8 | Novosphingobium tardaugens NBRC 16725 Putative hydrolase | Novosphingobium tardaugens NBRC 16725 |
| 9sphn-u3a0m8 | Novosphingobium tardaugens NBRC 16725 Uncharacterized protein | Novosphingobium tardaugens NBRC 16725 |
| 9sphn-w0a8l5 | Sphingomonas sanxanigenens DSM 19645 = NX02 Uncharacterized protein | Sphingomonas sanxanigenens DSM 19645 = NX02 |
| 9sphn-w0a9g1 | Sphingomonas sanxanigenens DSM 19645 = NX02 Uncharacterized protein | Sphingomonas sanxanigenens DSM 19645 = NX02 |
| 9xant-f0bgj0 | Xanthomonas vesicatoria ATCC 35937 Esterase\/lipase | Xanthomonas vesicatoria ATCC 35937 |
| 9xant-f0cdj1 | Xanthomonas gardneri ATCC 19865 Esterase\/lipase | Xanthomonas gardneri ATCC 19865 |
| 9xant-v7zi90 | Xanthomonas hortorum pv. carotae str. M081 Esterase | Xanthomonas hortorum pv. carotae str. M081 |
| 9xant-w4syz3 | Xanthomonas arboricola pv. pruni Exported acetyl esterase | Xanthomonas arboricola pv. pruni MAFF 301427 |
| 9zzzz-e6q0e6 | mine drainage metagenome Alpha\/beta hydrolase | mine drainage metagenome |
| acic5-c1f891 | Acidobacterium capsulatum (strain ATCC 51196 \/ DSM 11244 \/ JCM 7670) Esterase | Acidobacterium capsulatum |
| agaal-r9ptw5 | Agarivorans albus MKT 106 Esterase | Agarivorans albus MKT 106 |
| agrrk-b9jls9 | Agrobacterium radiobacter (strain K84 \/ ATCC BAA-868) Esterase\/lipase\/thioesterase family protein | Agrobacterium radiobacter |
| agrsh-f0lfk1 | Agrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3)) Esterase | Agrobacterium sp. |
| altma-j9ycg2 | Alteromonas macleodii ATCC 27126 Esterase | Alteromonas macleodii ATCC 27126 |
| altma-s5cfn7 | Alteromonas macleodii EH119 Esterase | Alteromonas macleodii |
| altmb-k0ej12 | Alteromonas macleodii (strain Balearic Sea AD45) Esterase | Alteromonas macleodii |
| altme-k0crz6 | Alteromonas macleodii (strain English Channel 673) Esterase | Alteromonas macleodii |
| altms-k0d8p8 | Alteromonas macleodii (strain Black Sea 11) Esterase | Alteromonas macleodii |
| altss-f5z698 | Alteromonas sp. (strain SN2) Esterase | Alteromonas sp. |
| asptn-q0cg08 | Aspergillus terreus (strain NIH 2624 \/ FGSC A1156) Predicted protein | Aspergillus terreus |
| burcj-b4ek59 | Burkholderia cenocepacia (Burkholderia cepacia) Putative esterase | Burkholderia cenocepacia |
| burs3-q396l8 | Burkholderia lata esterase\/lipase\/thioesterase | Burkholderia sp. |
| cauce-r0ekv3 | Caulobacter crescentus OR37 Esterase\/lipase | Caulobacter crescentus OR37 |
| caust-d5vhk7 | Caulobacter segnis (strain ATCC 21756 \/ DSM 7131 \/ JCM 7823 \/ NBR 15250 \/ LMG 17158 \/ TK0059) (Mycoplana segnis) Alpha\/beta hydrolase fold-3 domain protein | Caulobacter segnis |
| desao-e6vth1 | Desulfovibrio aespoeensis (strain ATCC 700646 \/ DSM 10631 \/ Aspo-2) Alpha\/beta hydrolase domain-containing protein | Desulfovibrio aespoeensis |
| frasn-a8leg3 | Frankia sp. (strain EAN1pec) Alpha\/beta hydrolase fold-3 domain protein | Frankia sp. |
| frasn-a8leg7 | Frankia sp. (strain EAN1pec) Alpha\/beta hydrolase fold-3 domain protein | Frankia sp. |
| frasn-a8lfl3 | Frankia sp. (strain EAN1pec) Alpha\/beta hydrolase fold-3 domain protein | Frankia sp. |
| frasu-e3ivg3 | Frankia sp. (strain EuI1c) Esterase\/lipase\/thioesterase | Frankia sp. |
| frasu-e3j816 | Frankia sp. (strain EuI1c) Esterase\/lipase\/thioesterase | Frankia sp. |
| frasu-e3j831 | Frankia sp. (strain EuI1c) Esterase\/lipase\/thioesterase family protein | Frankia sp. |
| frasu-e3j9a0 | Frankia sp. (strain EuI1c) Esterase | Frankia sp. |
| frasu-e3j9a7 | Frankia sp. (strain EuI1c) Esterase\/lipase\/thioesterase | Frankia sp. |
| fusox-w9hiy6 | Fusarium oxysporum Uncharacterized protein | Fusarium oxysporum FOSC 3-a |
| fusox-w9nxk0 | Fusarium oxysporum f. sp. pisi HDV247 Uncharacterized protein | Fusarium oxysporum f. sp. pisi HDV247 |
| fusox-x0h323 | Fusarium oxysporum f. sp. conglutinans race 2 54008 Uncharacterized protein | Fusarium oxysporum f. sp. conglutinans race 2 54008 |
| fusox-x0k2p3 | Fusarium oxysporum f. sp. cubense tropical race 4 54006 Uncharacterized protein | Fusarium oxysporum f. sp. cubense tropical race 4 54006 |
| fusox-x0m2c2 | Fusarium oxysporum f. sp. vasinfectum 25433 Uncharacterized protein | Fusarium oxysporum f. sp. vasinfectum 25433 |
| gibf5-s0ekc4 | Gibberella fujikuroi (strain CBS 195.34 \/ IMI 58289 \/ NRRL A-6831) (Bakanae and foot rot disease fungus) (Fusarium fujikuroi) Uncharacterized protein | Gibberella fujikuroi |
| gibm7-w7n497 | Gibberella moniliformis (strain M3125 \/ FGSC 7600) (Maize ear and stalk rot fungus) (Fusarium verticillioides) Uncharacterized protein | Gibberella moniliformis |
| grabc-q0br68 | Granulibacter bethesdensis Esterase | Granulibacter bethesdensis |
| grabc-q0br83 | Granulibacter bethesdensis (strain ATCC BAA-1260 \/ CGDNIH1) Esterase | Granulibacter bethesdensis |
| hypsm-f8jbt5 | Hyphomicrobium sp. (strain MC1) Alpha\/beta hydrolase fold-3 domain protein | Hyphomicrobium sp. |
| hypvg-g9nbv2 | Hypocrea virens (strain Gv29-8 \/ FGSC 10586) (Gliocladium virens) (Trichoderma virens) Uncharacterized protein | Hypocrea virens |
| korve-q1iuf6 | Koribacter versatilis (strain Ellin345) Alpha\/beta hydrolase | Koribacter versatilis |
| metno-b8iks9 | Methylobacterium nodulans (strain ORS2060 \/ LMG 21967) Alpha\/beta hydrolase fold-3 domain protein | Methylobacterium nodulans |
| metrj-b1lym6 | Methylobacterium radiotolerans (strain ATCC 27329 \/ DSM 1819 \/ JC 2831) Alpha\/beta hydrolase fold-3 domain protein | Methylobacterium radiotolerans |
| mets4-b0ud71 | Methylobacterium sp. (strain 4-46) Alpha\/beta hydrolase fold-3 domain protein | Methylobacterium sp. |
| mets4-b0und9 | Methylobacterium sp. (strain 4-46) Alpha\/beta hydrolase fold-3 domain protein | Methylobacterium sp. |
| novad-a4xew4 | Novosphingobium aromaticivorans (strain DSM 12444 \/ F199) Alpha\/beta hydrolase fold-3 domain protein | Novosphingobium aromaticivorans |
| psefl-v7ecg4 | Pseudomonas fluorescens\; Pseudomonas sp., Esterase\/lipase | Pseudomonas fluorescens BBc6R8 |
| rhird-h0hik8 | Agrobacterium tumefaciens 5A Esterase | Agrobacterium tumefaciens 5A |
| rhird-w1lea0 | Agrobacterium radiobacter DSM 30147 Esterase | Agrobacterium radiobacter DSM 30147 |
| rhitr-l0lr17 | Rhizobium tropici CIAT 899 Alpha\/beta hydrolase fold-3 catalytic domain-containing protein | Rhizobium tropici CIAT 899 |
| serp5-a8g8q2 | Serratia proteamaculans (strain 568) Alpha\/beta hydrolase fold-3 domain protein | Serratia proteamaculans |
| shehh-b0tmq3 | Shewanella halifaxensis (strain HAW-EB4) Alpha\/beta hydrolase fold-3 domain protein | Shewanella halifaxensis |
| shesh-a8ft15 | Shewanella sediminis (strain HAW-EB3) Esterase\/lipase-like protein | Shewanella sediminis |
| solue-q026h7 | Solibacter usitatus (strain Ellin6076) Alpha\/beta hydrolase | Solibacter usitatus |
| sphya-k9d6e0 | Sphingobium yanoikuyae ATCC 51230 Uncharacterized protein | Sphingobium yanoikuyae ATCC 51230 |
| synsp-ester | Synechocystis sp esterase | Synechocystis sp. |
| terrk-i3zbs2 | Terriglobus roseus (strain DSM 18391 \/ NRRL B-41598 \/ KBS 63) Esterase\/lipase | Terriglobus roseus |
| terrk-i3ze47 | Terriglobus roseus (strain DSM 18391 \/ NRRL B-41598 \/ KBS 63) Esterase\/lipase | Terriglobus roseus |
| vibna-s6lg09 | Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 Uncharacterized protein | Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 |
| vibph-w3zhu2 | Vibrio parahaemolyticus 50 Alpha\/beta hydrolase fold family protein | Vibrio parahaemolyticus 50 |
| vibsl-b7vqj9 | Vibrio splendidus (strain LGP32) (Vibrio splendidus (strain Mel32)) Putative esterase | Vibrio splendidus |
| xanca-XCC0080 | Xanthomonas campestris (pv. campestris) esterase | Xanthomonas campestris |
| xylfa-XF1743 | Xylella fastidiosa, gene XF1743 Esterase | Xylella fastidiosa |
| Gene_locus_frgt | Name | Species |
|---|---|---|
| 9bact-h6bdw2 | uncultured bacterium Lipolytic enzyme | uncultured bacterium |
| 9burk-b1t1g6 | Burkholderia ambifaria MEX-5 Alpha\/beta hydrolase fold-3 domain protein | Burkholderia ambifaria MEX-5 |
| 9mico-k1e0f4 | Janibacter hoylei PVAS-1 Alpha\/beta hydrolase domain-containing protein | Janibacter hoylei PVAS-1 |
| 9rhiz-k2qkb8 | Agrobacterium albertimagni AOL15 Esterase\/lipase\/thioesterase family protein | Agrobacterium albertimagni AOL15 |
| 9rhiz-w6s8s5 | Rhizobium sp. LPU83 Esterase\/lipase\/thioesterase family protein | Rhizobium sp. LPU83 |
| 9sphn-g2iq94 | Sphingobium sp. SYK-6 Putative esterase | Sphingobium sp. SYK-6 |
| 9zzzz-x0t7r8 | marine sediment metagenome Marine sediment metagenome DNA, contig: S01H1_L12205 | marine sediment metagenome |
| frant-i2b041 | Francisella noatunensis subsp. orientalis (strain Toba 04) Alpha\/beta hydrolase domain-containing protein | Francisella noatunensis subsp. orientalis |
| mycne-v5xdk1 | Mycobacterium neoaurum VKM Ac-1815D Alpha\/beta hydrolase | Mycobacterium neoaurum VKM Ac-1815D |
| Inhibitor | Chemical Nomenclature | Proteins |
|---|---|---|
| PMSF | Phenylmethylsulfonyl fluoride | theet-q3ch51 emeni-q5avy9 human-ABHD10 human-PREPL myctu-Rv1430 psefl-cxest 9bact-E40 thefu-q6a0i4 ecoli-Aes orysj-Q10QA5 bac25-mglp 9bact-Q0GMU2 bacsu-FENB psepu-clcd1 thema-TM0077 bacsu-RsbQ ecoli-bioh drome-CG9542 human-FAP tubmm-TmEst2 human-NOTUM cutac-e6dae5 9gamm-urc15994 9baci-EstD9 geost-est30 pholl-AntI |
References (5)
| Title : Crystal structure of PMGL2 esterase from the hormone-sensitive lipase family with GCSAG motif around the catalytic serine - Boyko_2020_PLoS.One_15_e0226838 |
| Author(s) : Boyko KM , Kryukova MV , Petrovskaya LE , Nikolaeva AY , Korzhenevsky DA , Novototskaya-Vlasova KA , Rivkina EM , Dolgikh DA , Kirpichnikov MP , Popov VO |
| Ref : PLoS ONE , 15 :e0226838 , 2020 |
| Abstract : |
| PubMedSearch : Boyko_2020_PLoS.One_15_e0226838 |
| PubMedID: 31990908 |
| Gene_locus related to this paper: 9bact-a0a142j6i6 |
| Title : Expression and characterization of a new esterase with GCSAG motif from a permafrost metagenomic library - Petrovskaya_2016_FEMS.Microbiol.Ecol_92_ |
| Author(s) : Petrovskaya LE , Novototskaya-Vlasova KA , Spirina EV , Durdenko EV , Lomakina GY , Zavialova MG , Nikolaev EN , Rivkina EM |
| Ref : FEMS Microbiol Ecol , 92 : , 2016 |
| Abstract : |
| PubMedSearch : Petrovskaya_2016_FEMS.Microbiol.Ecol_92_ |
| PubMedID: 26929439 |
| Gene_locus related to this paper: 9bact-a0a142j6i6 |
| Title : Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats - Alcaide_2015_Environ.Microbiol_17_332 |
| Author(s) : Alcaide M , Stogios PJ , Lafraya A , Tchigvintsev A , Flick R , Bargiela R , Chernikova TN , Reva ON , Hai T , Leggewie CC , Katzke N , La Cono V , Matesanz R , Jebbar M , Jaeger KE , Yakimov MM , Yakunin AF , Golyshin PN , Golyshina OV , Savchenko A , Ferrer M |
| Ref : Environ Microbiol , 17 :332 , 2015 |
| Abstract : |
| PubMedSearch : Alcaide_2015_Environ.Microbiol_17_332 |
| PubMedID: 25330254 |
| Gene_locus related to this paper: 9alte-MGS-MT1 , 9bact-MGS-M1 , 9bact-MGS-M2 , 9bact-a0a0b5kns5 |
| Title : Structural Basis for Dimerization and Catalysis of a Novel Esterase from the GTSAG Motif Subfamily of the Bacterial Hormone-sensitive Lipase Family - Li_2014_J.Biol.Chem_289_19031 |
| Author(s) : Li PY , Ji P , Li CY , Zhang Y , Wang GL , Zhang XY , Xie BB , Qin QL , Chen XL , Zhou BC , Zhang YZ |
| Ref : Journal of Biological Chemistry , 289 :19031 , 2014 |
| Abstract : |
| PubMedSearch : Li_2014_J.Biol.Chem_289_19031 |
| PubMedID: 24867954 |
| Gene_locus related to this paper: 9bact-E25 |
| Title : Identification of a new subfamily of salt-tolerant esterases from a metagenomic library of tidal flat sediment - Jeon_2012_Appl.Microbiol.Biotechnol_93_623 |
| Author(s) : Jeon JH , Lee HS , Kim JT , Kim SJ , Choi SH , Kang SG , Lee JH |
| Ref : Applied Microbiology & Biotechnology , 93 :623 , 2012 |
| Abstract : |
| PubMedSearch : Jeon_2012_Appl.Microbiol.Biotechnol_93_623 |
| PubMedID: 21720822 |
| Gene_locus related to this paper: 9bact-E40 , 9bact-E25 , 9bact-d8v1j5 , 9bact-d8v1j4 , 9bact-d8v1j3 |