Paranitrophenylacetate
Substrate used in assays for esterase and lipase activity. The release of p-nitrophenol is determined at 405 nm. The molar extinction coefficient for p-nitrophenol is 18,000 M-1 cm-1
General
Type : pNP, Chromogen, Aryl ester, Acetate
Chemical_Nomenclature : (4-nitrophenyl) acetate
Canonical SMILES : CC(=O)OC1=CC=C(C=C1)[N+](=O)[O-]
InChI : InChI=1S\/C8H7NO4\/c1-6(10)13-8-4-2-7(3-5-8)9(11)12\/h2-5H,1H3
InChIKey : QAUUDNIGJSLPSX-UHFFFAOYSA-N
Other name(s) : pNPA || p-NPA || pNPA2 || 4-NPA || pNP-C2 || pNPC2 || pNP2 || 4-Nitrophenyl acetate || P-Acetoxynitrobenzene || P-Nitrophenol acetate || p-nitrophenyl acetate || p-nitrophenyl-acetate || pNP-acetate || pNP acetate || p-NP acetate || Acetic acid, 4-nitrophenyl ester
MW : 181.15
Formula : C8H7NO4
CAS_number : 830-03-5
PubChem : 13243
UniChem : QAUUDNIGJSLPSX-UHFFFAOYSA-N
Target
Structures : No structure
Families : BCHE, Carb_B_Bacteria, Carb_B_Chordata, Carboxylesterase, ACHE, GTSAGmotif, A85-EsteraseD-FGH, Homoserine_transacetylase_like_est, Bacterial_lip_FamI.5, HNLyase_Bact, BioH, Est9X, Pectinacetylesterase-Notum, Acetyl-esterase_deacetylase, Hydroxynitrile_lyase, 6_AlphaBeta_hydrolase, Fungal_carboxylesterase_lipase, A85-Feruloyl-Esterase, LYsophospholipase_carboxylesterase, FaeC, Cutinase, CarbLipBact_1, Bacterial_esterase, Lipase_3, CIB-CCG1-interacting-factor-B, Monoglyceridelipase_lysophospholip
References (23)
| Title : A novel thermostable and salt-tolerant carboxylesterase involved in the initial aerobic degradation pathway for pyrethroids in Glycomyces salinus - Liu_2023_J.Hazard.Mater_451_131128 |
| Author(s) : Liu Y , Tang S , Wang X , Tang X , Wu Q , Huang Z , Ding J |
| Ref : J Hazard Mater , 451 :131128 , 2023 |
| Abstract : |
| PubMedSearch : Liu_2023_J.Hazard.Mater_451_131128 |
| PubMedID: 36893599 |
| Gene_locus related to this paper: 9actn-EstGS1 |
| Title : Crystal structures of herbicide-detoxifying esterase reveal a lid loop affecting substrate binding and activity - Liu_2023_Nat.Commun_14_4343 |
| Author(s) : Liu B , Wang W , Qiu J , Huang X , Qiu S , Bao Y , Xu S , Ruan L , Ran T , He J |
| Ref : Nat Commun , 14 :4343 , 2023 |
| Abstract : |
| PubMedSearch : Liu_2023_Nat.Commun_14_4343 |
| PubMedID: 37468532 |
| Gene_locus related to this paper: 9rhiz-g9i933 |
| Title : Characterization of a carboxylesterase with hyper-thermostability and alkali-stability from Streptomyces lividans TK24 - Chang_2021_Extremophiles__ |
| Author(s) : Chang X , Wu S , Chen J , Xiong S , Wang P , Shi X , Wang A , Wang B |
| Ref : Extremophiles , : , 2021 |
| Abstract : |
| PubMedSearch : Chang_2021_Extremophiles__ |
| PubMedID: 33515353 |
| Gene_locus related to this paper: strco-SCO2123 |
| Title : Phylogenetic analysis and in-depth characterization of functionally and structurally diverse CE5 cutinases - Novy_2021_J.Biol.Chem__101302 |
| Author(s) : Novy V , Carneiro LV , Shin JH , Larsbrink J , Olsson L |
| Ref : Journal of Biological Chemistry , :101302 , 2021 |
| Abstract : |
| PubMedSearch : Novy_2021_J.Biol.Chem__101302 |
| PubMedID: 34653507 |
| Gene_locus related to this paper: 9pezi-s4vch4 , aspfu-q4x1n0 , crysp-Q874E9 , emeni-q5b2c1 , fusso-cutas , pyrbr-Q9Y7G8 , copci-b9u443 , thite-g2rae6 , strsw-c9zcr8 |
| Title : Functional Annotation of ABHD14B, an Orphan Serine Hydrolase Enzyme - Rajendran_2020_Biochemistry_59_183 |
| Author(s) : Rajendran A , Vaidya K , Mendoza J , Bridwell-Rabb J , Kamat SS |
| Ref : Biochemistry , 59 :183 , 2020 |
| Abstract : |
| PubMedSearch : Rajendran_2020_Biochemistry_59_183 |
| PubMedID: 31478652 |
| Gene_locus related to this paper: human-CIB |
| Title : Characterization of a novel hyper-thermostable and chlorpyrifos-hydrolyzing carboxylesterase EstC: A representative of the new esterase family XIX - Wang_2020_Pestic.Biochem.Physiol_170_104704 |
| Author(s) : Wang B , Wu S , Chang X , Chen J , Ma J , Wang P , Zhu G |
| Ref : Pestic Biochem Physiol , 170 :104704 , 2020 |
| Abstract : |
| PubMedSearch : Wang_2020_Pestic.Biochem.Physiol_170_104704 |
| PubMedID: 32980065 |
| Gene_locus related to this paper: strco-estli |
| Title : Biochemical and Structural Analyses of Two Cryptic Esterases in Bacteroides intestinalis and their Synergistic Activities with Cognate Xylanases - Wefers_2017_J.Mol.Biol_429_2509 |
| Author(s) : Wefers D , Cavalcante JJV , Schendel RR , Deveryshetty J , Wang K , Wawrzak Z , Mackie RI , Koropatkin NM , Cann I |
| Ref : Journal of Molecular Biology , 429 :2509 , 2017 |
| Abstract : |
| PubMedSearch : Wefers_2017_J.Mol.Biol_429_2509 |
| PubMedID: 28669823 |
| Gene_locus related to this paper: 9bace-b3c9d0.2 , 9bace-b3cet1 |
| Title : Accurately determining esterase activity via the isosbestic point of p-nitrophenol - Peng_2016_BioRes_11_10099 |
| Author(s) : Peng Y , Fu S , Liu H , Lucia LA |
| Ref : BioRes , 11 :10099 , 2016 |
| Abstract : |
| PubMedSearch : Peng_2016_BioRes_11_10099 |
| PubMedID: |
| Title : An extended loop in CE7 carbohydrate esterase family is dispensable for oligomerization but required for activity and thermostability - Singh_2016_J.Struct.Biol_194_434 |
| Author(s) : Singh MK , Manoj N |
| Ref : J Struct Biol , 194 :434 , 2016 |
| Abstract : |
| PubMedSearch : Singh_2016_J.Struct.Biol_194_434 |
| PubMedID: 27085421 |
| Gene_locus related to this paper: thema-TM0077 |
| Title : Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search - Porter_2015_ACS.Chem.Biol_10_611 |
| Author(s) : Porter JL , Boon PL , Murray TP , Huber T , Collyer CA , Ollis DL |
| Ref : ACS Chemical Biology , 10 :611 , 2015 |
| Abstract : |
| PubMedSearch : Porter_2015_ACS.Chem.Biol_10_611 |
| PubMedID: 25419863 |
| Gene_locus related to this paper: psepu-clcd1 |
| Title : A role for His-160 in peroxide inhibition of S. cerevisiae S-formylglutathione hydrolase: evidence for an oxidation sensitive motif - Legler_2012_Arch.Biochem.Biophys_528_7 |
| Author(s) : Legler PM , Leary DH , Hervey WJt , Millard CB |
| Ref : Archives of Biochemistry & Biophysics , 528 :7 , 2012 |
| Abstract : |
| PubMedSearch : Legler_2012_Arch.Biochem.Biophys_528_7 |
| PubMedID: 22906720 |
| Gene_locus related to this paper: yeast-yjg8 |
| Title : SulE, a sulfonylurea herbicide de-esterification esterase from Hansschlegelia zhihuaiae S113 - Hang_2012_Appl.Environ.Microbiol_78_1962 |
| Author(s) : Hang BJ , Hong Q , Xie XT , Huang X , Wang CH , He J , Li SP |
| Ref : Applied Environmental Microbiology , 78 :1962 , 2012 |
| Abstract : |
| PubMedSearch : Hang_2012_Appl.Environ.Microbiol_78_1962 |
| PubMedID: 22247165 |
| Gene_locus related to this paper: 9rhiz-g9i933 |
| Title : Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated by several esterases belonging to the AtMES esterase family of Arabidopsis - Yang_2008_Plant.Physiol_147_1034 |
| Author(s) : Yang Y , Xu R , Ma CJ , Vlot AC , Klessig DF , Pichersky E |
| Ref : Plant Physiol , 147 :1034 , 2008 |
| Abstract : |
| PubMedSearch : Yang_2008_Plant.Physiol_147_1034 |
| PubMedID: 18467465 |
| Gene_locus related to this paper: arath-MES17 , arath-AT4G16690 , arath-AT4G37150 , arath-MES6 , arath-MES7 , arath-MES3 , arath-MES1 , arath-MES18 , arath-MES10 , arath-MES19 |
| Title : Degradation of alkyl methyl ketones by Pseudomonas veronii MEK700 - Onaca_2007_J.Bacteriol_189_3759 |
| Author(s) : Onaca C , Kieninger M , Engesser KH , Altenbuchner J |
| Ref : Journal of Bacteriology , 189 :3759 , 2007 |
| Abstract : |
| PubMedSearch : Onaca_2007_J.Bacteriol_189_3759 |
| PubMedID: 17351032 |
| Gene_locus related to this paper: 9psed-q0mrg5 |
| Title : Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls - Bauer_2006_Proc.Natl.Acad.Sci.U.S.A_103_11417 |
| Author(s) : Bauer S , Vasu P , Persson S , Mort AJ , Somerville CR |
| Ref : Proc Natl Acad Sci U S A , 103 :11417 , 2006 |
| Abstract : |
| PubMedSearch : Bauer_2006_Proc.Natl.Acad.Sci.U.S.A_103_11417 |
| PubMedID: 16844780 |
| Gene_locus related to this paper: emeni-axe1 , emeni-CUTI3 , emeni-faec |
| Title : Isolation, characterization, and heterologous expression of a carboxylesterase of Pseudomonas aeruginosa PAO1 - Pesaresi_2005_Curr.Microbiol_50_102 |
| Author(s) : Pesaresi A , Devescovi G , Lamba D , Venturi V , Degrassi G |
| Ref : Curr Microbiol , 50 :102 , 2005 |
| Abstract : |
| PubMedSearch : Pesaresi_2005_Curr.Microbiol_50_102 |
| PubMedID: 15717224 |
| Gene_locus related to this paper: pseae-PA3859 |
| Title : Cloning and characterization of EstC from Burkholderia gladioli, a novel-type esterase related to plant enzymes - Reiter_2000_Appl.Microbiol.Biotechnol_54_778 |
| Author(s) : Reiter B , Glieder A , Talker D , Schwab H |
| Ref : Applied Microbiology & Biotechnology , 54 :778 , 2000 |
| Abstract : |
| PubMedSearch : Reiter_2000_Appl.Microbiol.Biotechnol_54_778 |
| PubMedID: 11152069 |
| Gene_locus related to this paper: burgl-EstC |
| Title : Electrostatic influence on the kinetics of ligand binding to acetylcholinesterase. Distinctions between active center ligands and fasciculin - Radic_1997_J.Biol.Chem_272_23265 |
| Author(s) : Radic Z , Kirchhoff PD , Quinn DM , McCammon JA , Taylor P |
| Ref : Journal of Biological Chemistry , 272 :23265 , 1997 |
| Abstract : |
| PubMedSearch : Radic_1997_J.Biol.Chem_272_23265 |
| PubMedID: 9287336 |
| Title : Purification and partial characterization of a novel thermophilic carboxylesterase with high mesophilic specific activity - Wood_1995_Enzyme.Microb.Technol_17_816 |
| Author(s) : Wood AN , Fernandez-Lafuente R , Cowan DA |
| Ref : Enzyme Microb Technol , 17 :816 , 1995 |
| Abstract : |
| PubMedSearch : Wood_1995_Enzyme.Microb.Technol_17_816 |
| PubMedID: 7576531 |
| Gene_locus related to this paper: geost-est50 |
| Title : Dissection of the human acetylcholinesterase active center determinants of substrate specificity. Identification of residues constituting the anionic site, the hydrophobic site, and the acyl pocket - Ordentlich_1993_J.Biol.Chem_268_17083 |
| Author(s) : Ordentlich A , Barak D , Kronman C , Flashner Y , Leitner M , Segall Y , Ariel N , Cohen S , Velan B , Shafferman A |
| Ref : Journal of Biological Chemistry , 268 :17083 , 1993 |
| Abstract : |
| PubMedSearch : Ordentlich_1993_J.Biol.Chem_268_17083 |
| PubMedID: 8349597 |
| Gene_locus related to this paper: human-ACHE , human-BCHE |
| Title : Catalysis by acetylcholinesterase in two-hydronic-reactive states. Integrity of deuterium oxide effects and hydron inventories - Salih_1992_Biochem.J_285_451 |
| Author(s) : Salih E |
| Ref : Biochemical Journal , 285 :451 , 1992 |
| Abstract : |
| PubMedSearch : Salih_1992_Biochem.J_285_451 |
| PubMedID: 1322133 |
| Title : The nucleotide and deduced amino acid sequences of porcine liver proline- beta-naphthylamidase. Evidence for the identity with carboxylesterase - Matsushima_1991_FEBS.Lett_293_37 |
| Author(s) : Matsushima M , Inoue H , Ichinose M , Tsukada S , Miki K , Kurokawa K , Takahashi T , Takahashi K |
| Ref : FEBS Letters , 293 :37 , 1991 |
| Abstract : |
| PubMedSearch : Matsushima_1991_FEBS.Lett_293_37 |
| PubMedID: 1959668 |
| Gene_locus related to this paper: pig-EST1 |
| Title : Catalysis by acetylcholinesterase: evidence that the rate-limiting step for acylation with certain substrates precedes general acid-base catalysis - Rosenberry_1975_Proc.Natl.Acad.Sci.U.S.A_72_3834 |
| Author(s) : Rosenberry TL |
| Ref : Proceedings of the National Academy of Sciences of the United States of America , 72 :3834 , 1975 |
| Abstract : |
| PubMedSearch : Rosenberry_1975_Proc.Natl.Acad.Sci.U.S.A_72_3834 |
| PubMedID: 668 |