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Family Report for: Duf_2235


Family Duf_2235
Block X
The type VI lipase effectors Tle1-Tle5 secreted by the bacterial type VI secretion system (T6SS) have recently been identified as antibacterial effectors that hydrolyze membrane phospholipids. The Tle1-4 display phospholipase A1 and A2 (PLA1) (PLA2) activities. Tle1 belongs to Duf_2235 family, TLE2 to Lipase_3, TLE3 to alpha/ beta hydrolase and Tle4 belongs to PGAP1. The adjacent genes code for VgrG proteins and putative periplasmic immunity proteins Tle5 are unrelated phospholipase D type. The bacterial type VI secretion system (T6SS) is a macromolecular machine that injects effectors into prokaryotic and eukaryotic cells. In enteroaggregative Escherichia coli, the Tle1 phospholipase binds the C-terminal extension of the VgrG trimeric spike. The inhibition of Tle1 phospholipase activity once bound to VgrG suggests that Tle1 dissociation from VgrG is required upon delivery

IPR018712 (Protein of unknown function DUF2235)
PF09994 (DUF2235)
no EC number

Peptide in
Nucleotide in
Alignment with Multalin
|Text only/graphic display
Seed alignment with MAFFT
|No colour/coloured with Mview
Alignment with MAFFT
|No colour/coloured with Mview
|Graphical display, obtained with the dnd file produced by Clustalw

    Title: Structural basis for loading and inhibition of a bacterial T6SS phospholipase effector by the VgrG spike
    Flaugnatti N, Rapisarda C, Rey M, Beauvois SG, Nguyen VA, Canaan S, Durand E, Chamot-Rooke J, Cascales E and Journet L <1 more author(s)>
    Ref: EMBO Journal, 39:e104129, 2020 : PubMed


    Title: Structure of the type VI secretion phospholipase effector Tle1 provides insight into its hydrolysis and membrane targeting
    Hu H, Zhang H, Gao Z, Wang D, Liu G, Xu J, Lan K, Dong Y
    Ref: Acta Crystallographica D Biol Crystallogr, 70:2175, 2014 : PubMed


    Title: Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors
    Russell AB, LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD
    Ref: Nature, 496:508, 2013 : PubMed


Other Papers

No structure scheme yet for this family

Structures in Duf_2235 family (3)

Genes Proteins in Duf_2235 family (33)

Fragments of genes in Duf_2235 family (1)

Substrates of some enzymes in the Duf_2235 family (1)

No Inhibitor

Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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