Weidman J

References (12)

Title : Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain - Gill_2005_J.Bacteriol_187_2426
Author(s) : Gill SR , Fouts DE , Archer GL , Mongodin EF , DeBoy RT , Ravel J , Paulsen IT , Kolonay JF , Brinkac L , Beanan M , Dodson RJ , Daugherty SC , Madupu R , Angiuoli SV , Durkin AS , Haft DH , Vamathevan J , Khouri H , Utterback T , Lee C , Dimitrov G , Jiang L , Qin H , Weidman J , Tran K , Kang K , Hance IR , Nelson KE , Fraser CM
Ref : Journal of Bacteriology , 187 :2426 , 2005
Abstract : Staphylococcus aureus is an opportunistic pathogen and the major causative agent of numerous hospital- and community-acquired infections. Staphylococcus epidermidis has emerged as a causative agent of infections often associated with implanted medical devices. We have sequenced the approximately 2.8-Mb genome of S. aureus COL, an early methicillin-resistant isolate, and the approximately 2.6-Mb genome of S. epidermidis RP62a, a methicillin-resistant biofilm isolate. Comparative analysis of these and other staphylococcal genomes was used to explore the evolution of virulence and resistance between these two species. The S. aureus and S. epidermidis genomes are syntenic throughout their lengths and share a core set of 1,681 open reading frames. Genome islands in nonsyntenic regions are the primary source of variations in pathogenicity and resistance. Gene transfer between staphylococci and low-GC-content gram-positive bacteria appears to have shaped their virulence and resistance profiles. Integrated plasmids in S. epidermidis carry genes encoding resistance to cadmium and species-specific LPXTG surface proteins. A novel genome island encodes multiple phenol-soluble modulins, a potential S. epidermidis virulence factor. S. epidermidis contains the cap operon, encoding the polyglutamate capsule, a major virulence factor in Bacillus anthracis. Additional phenotypic differences are likely the result of single nucleotide polymorphisms, which are most numerous in cell envelope proteins. Overall differences in pathogenicity can be attributed to genome islands in S. aureus which encode enterotoxins, exotoxins, leukocidins, and leukotoxins not found in S. epidermidis.
ESTHER : Gill_2005_J.Bacteriol_187_2426
PubMedSearch : Gill_2005_J.Bacteriol_187_2426
PubMedID: 15774886
Gene_locus related to this paper: staau-LIP , staau-lipas , staau-MW0741 , staau-MW2456 , staau-q6gfm6 , staau-SA0011 , staau-SA0569 , staau-SA0572 , staau-SA0897 , staau-SA1143 , staau-SA2240 , staau-SA2306 , staau-SA2367 , staau-SA2422 , staau-SAV0321 , staau-SAV0446 , staau-SAV0457 , staau-SAV0655 , staau-SAV1014 , staau-SAV1765 , staau-SAV1793 , staau-SAV2188 , staau-SAV2350 , staau-SAV2484 , staau-SAV2594 , staep-lipas , staep-SE0011 , staep-SE0226 , staep-SE0386 , staep-SE0389 , staep-SE0424 , staep-SE0564 , staep-SE0714 , staep-SE0745 , staep-SE0980 , staep-SE1436 , staep-SE1460 , staep-SE1510 , staep-SE1780 , staep-SE1929 , staep-SERP2035 , staep-SE2050 , staep-SE2095 , staep-SE2213 , staep-SE2328

Title : The genome of Salinibacter ruber: convergence and gene exchange among hyperhalophilic bacteria and archaea - Mongodin_2005_Proc.Natl.Acad.Sci.U.S.A_102_18147
Author(s) : Mongodin EF , Nelson KE , Daugherty S , DeBoy RT , Wister J , Khouri H , Weidman J , Walsh DA , Papke RT , Sanchez Perez G , Sharma AK , Nesbo CL , Macleod D , Bapteste E , Doolittle WF , Charlebois RL , Legault B , Rodriguez-Valera F
Ref : Proc Natl Acad Sci U S A , 102 :18147 , 2005
Abstract : Saturated thalassic brines are among the most physically demanding habitats on Earth: few microbes survive in them. Salinibacter ruber is among these organisms and has been found repeatedly in significant numbers in climax saltern crystallizer communities. The phenotype of this bacterium is remarkably similar to that of the hyperhalophilic Archaea (Haloarchaea). The genome sequence suggests that this resemblance has arisen through convergence at the physiological level (different genes producing similar overall phenotype) and the molecular level (independent mutations yielding similar sequences or structures). Several genes and gene clusters also derive by lateral transfer from (or may have been laterally transferred to) haloarchaea. S. ruber encodes four rhodopsins. One resembles bacterial proteorhodopsins and three are of the haloarchaeal type, previously uncharacterized in a bacterial genome. The impact of these modular adaptive elements on the cell biology and ecology of S. ruber is substantial, affecting salt adaptation, bioenergetics, and photobiology.
ESTHER : Mongodin_2005_Proc.Natl.Acad.Sci.U.S.A_102_18147
PubMedSearch : Mongodin_2005_Proc.Natl.Acad.Sci.U.S.A_102_18147
PubMedID: 16330755
Gene_locus related to this paper: salrd-q2ryx4 , salrd-q2ryz7 , salrd-q2rz15 , salrd-q2rz19 , salrd-q2rz52 , salrd-q2rz84 , salrd-q2rzy1 , salrd-q2s0e4 , salrd-q2s1i8 , salrd-q2s1v7 , salrd-q2s1v9 , salrd-q2s2d6 , salrd-q2s2y7 , salrd-q2s5a6 , salrd-q2s5k5 , salrd-q2s5u7 , salrd-q2s6g6 , salrd-q2s039 , salrd-q2s117 , salrd-q2s215 , salrd-q2s287 , salrd-q2s400 , salrd-q2s402 , salrd-q2s473 , salrd-q2s516 , salrd-q2s564

Title : Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes - Gardner_2005_Science_309_134
Author(s) : Gardner MJ , Bishop R , Shah T , de Villiers EP , Carlton JM , Hall N , Ren Q , Paulsen IT , Pain A , Berriman M , Wilson RJ , Sato S , Ralph SA , Mann DJ , Xiong Z , Shallom SJ , Weidman J , Jiang L , Lynn J , Weaver B , Shoaibi A , Domingo AR , Wasawo D , Crabtree J , Wortman JR , Haas B , Angiuoli SV , Creasy TH , Lu C , Suh B , Silva JC , Utterback TR , Feldblyum TV , Pertea M , Allen J , Nierman WC , Taracha EL , Salzberg SL , White OR , Fitzhugh HA , Morzaria S , Venter JC , Fraser CM , Nene V
Ref : Science , 309 :134 , 2005
Abstract : We report the genome sequence of Theileria parva, an apicomplexan pathogen causing economic losses to smallholder farmers in Africa. The parasite chromosomes exhibit limited conservation of gene synteny with Plasmodium falciparum, and its plastid-like genome represents the first example where all apicoplast genes are encoded on one DNA strand. We tentatively identify proteins that facilitate parasite segregation during host cell cytokinesis and contribute to persistent infection of transformed host cells. Several biosynthetic pathways are incomplete or absent, suggesting substantial metabolic dependence on the host cell. One protein family that may generate parasite antigenic diversity is not telomere-associated.
ESTHER : Gardner_2005_Science_309_134
PubMedSearch : Gardner_2005_Science_309_134
PubMedID: 15994558
Gene_locus related to this paper: thepa-q4mzr2 , thepa-q4n0b4 , thepa-q4n2i4 , thepa-q4n4i8 , thepa-q4n5d6 , thepa-q4n5m4 , thepa-q4n006 , thepa-q4n9g7 , thepa-q4n315 , thepa-q4n349 , thepa-q4n803

Title : Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5 - Paulsen_2005_Nat.Biotechnol_23_873
Author(s) : Paulsen IT , Press CM , Ravel J , Kobayashi DY , Myers GS , Mavrodi DV , DeBoy RT , Seshadri R , Ren Q , Madupu R , Dodson RJ , Durkin AS , Brinkac LM , Daugherty SC , Sullivan SA , Rosovitz MJ , Gwinn ML , Zhou L , Schneider DJ , Cartinhour SW , Nelson WC , Weidman J , Watkins K , Tran K , Khouri H , Pierson EA , Pierson LS, 3rd , Thomashow LS , Loper JE
Ref : Nat Biotechnol , 23 :873 , 2005
Abstract : Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified various features that contribute to its commensal lifestyle on plants, including broad catabolic and transport capabilities for utilizing plant-derived compounds, the apparent ability to use a diversity of iron siderophores, detoxification systems to protect from oxidative stress, and the lack of a type III secretion system and toxins found in related pathogens. In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5.
ESTHER : Paulsen_2005_Nat.Biotechnol_23_873
PubMedSearch : Paulsen_2005_Nat.Biotechnol_23_873
PubMedID: 15980861
Gene_locus related to this paper: psef5-metx , psef5-q4k3c9 , psef5-q4k4b4 , psef5-q4k4t4 , psef5-q4k4u7 , psef5-q4k4y2 , psef5-q4k5b5 , psef5-q4k5k6 , psef5-q4k5w4 , psef5-q4k6z9 , psef5-q4k7i6 , psef5-q4k7u9 , psef5-q4k8j2 , psef5-q4k9i3 , psef5-q4k458 , psef5-q4k713 , psef5-q4k717 , psef5-q4k873 , psef5-q4k906 , psef5-q4k982 , psef5-q4k989 , psef5-q4k993 , psef5-q4kax4 , psef5-q4kay8 , psef5-q4kaz0 , psef5-q4kaz4 , psef5-q4kb21 , psef5-q4kbd7 , psef5-q4kbs3 , psef5-q4kbs6 , psef5-q4kc18 , psef5-q4kc21 , psef5-q4kcd3 , psef5-q4kch8 , psef5-q4kcj3 , psef5-q4kck4 , psef5-q4kcn8 , psef5-q4kcq2 , psef5-q4kcx3 , psef5-q4kd54 , psef5-q4kda1 , psef5-q4kdb4 , psef5-q4ke18 , psef5-q4keh1 , psef5-q4kej0 , psef5-q4keq4 , psef5-q4kes9 , psef5-q4kf14 , psef5-q4kfj4 , psef5-q4kfw0 , psef5-q4kfw1 , psef5-q4kfx7 , psef5-q4kgg3 , psef5-q4kgj9 , psef5-q4kgs6 , psef5-q4kh30 , psef5-q4kha2 , psef5-q4khf1 , psef5-q4khl0 , psef5-q4khv5 , psef5-q4ki42 , psef5-q4kj24 , psef5-q4kj95 , psef5-q4kjk5 , psef5-q4kjk7 , psef5-q4kjm8 , psef5-q4kjt7 , psef5-q4kk20 , psef5-q4kk22 , psef5-q4kk59 , psef5-q4kkf7 , psefl-PLTG , psepf-PHAZ , psef5-q4kfd8

Title : Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus - Nierman_2005_Nature_438_1151
Author(s) : Nierman WC , Pain A , Anderson MJ , Wortman JR , Kim HS , Arroyo J , Berriman M , Abe K , Archer DB , Bermejo C , Bennett J , Bowyer P , Chen D , Collins M , Coulsen R , Davies R , Dyer PS , Farman M , Fedorova N , Feldblyum TV , Fischer R , Fosker N , Fraser A , Garcia JL , Garcia MJ , Goble A , Goldman GH , Gomi K , Griffith-Jones S , Gwilliam R , Haas B , Haas H , Harris D , Horiuchi H , Huang J , Humphray S , Jimenez J , Keller N , Khouri H , Kitamoto K , Kobayashi T , Konzack S , Kulkarni R , Kumagai T , Lafon A , Latge JP , Li W , Lord A , Lu C , Majoros WH , May GS , Miller BL , Mohamoud Y , Molina M , Monod M , Mouyna I , Mulligan S , Murphy L , O'Neil S , Paulsen I , Penalva MA , Pertea M , Price C , Pritchard BL , Quail MA , Rabbinowitsch E , Rawlins N , Rajandream MA , Reichard U , Renauld H , Robson GD , Rodriguez de Cordoba S , Rodriguez-Pena JM , Ronning CM , Rutter S , Salzberg SL , Sanchez M , Sanchez-Ferrero JC , Saunders D , Seeger K , Squares R , Squares S , Takeuchi M , Tekaia F , Turner G , Vazquez de Aldana CR , Weidman J , White O , Woodward J , Yu JH , Fraser C , Galagan JE , Asai K , Machida M , Hall N , Barrell B , Denning DW
Ref : Nature , 438 :1151 , 2005
Abstract : Aspergillus fumigatus is exceptional among microorganisms in being both a primary and opportunistic pathogen as well as a major allergen. Its conidia production is prolific, and so human respiratory tract exposure is almost constant. A. fumigatus is isolated from human habitats and vegetable compost heaps. In immunocompromised individuals, the incidence of invasive infection can be as high as 50% and the mortality rate is often about 50% (ref. 2). The interaction of A. fumigatus and other airborne fungi with the immune system is increasingly linked to severe asthma and sinusitis. Although the burden of invasive disease caused by A. fumigatus is substantial, the basic biology of the organism is mostly obscure. Here we show the complete 29.4-megabase genome sequence of the clinical isolate Af293, which consists of eight chromosomes containing 9,926 predicted genes. Microarray analysis revealed temperature-dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype. The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus.
ESTHER : Nierman_2005_Nature_438_1151
PubMedSearch : Nierman_2005_Nature_438_1151
PubMedID: 16372009
Gene_locus related to this paper: aspfc-b0xp50 , aspfc-b0xu40 , aspfc-b0xzj6 , aspfc-dpp5 , aspfu-apth1 , aspfu-axe1 , aspfu-CBPYA , aspfu-faec , aspfu-kex1 , aspfu-ppme1 , aspfu-q4wa39 , aspfu-q4wa78 , aspfu-q4wf56 , aspfu-q4wg73 , aspfu-q4wk44 , aspfu-q4wkh6 , aspfu-q4wnx3 , aspfu-q4wpb9 , aspfu-q4wqv2 , aspfu-q4wub2 , aspfu-q4wxr1 , aspfu-q4x0n6 , aspfu-q4x1n0 , aspfu-q5vjg7 , neofi-a1cwa6 , neofi-a1dfr9 , aspfm-a0a084bf80 , aspfu-fmac

Title : Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements - Wu_2004_PLoS.Biol_2_E69
Author(s) : Wu M , Sun LV , Vamathevan J , Riegler M , Deboy R , Brownlie JC , McGraw EA , Martin W , Esser C , Ahmadinejad N , Wiegand C , Madupu R , Beanan MJ , Brinkac LM , Daugherty SC , Durkin AS , Kolonay JF , Nelson WC , Mohamoud Y , Lee P , Berry K , Young MB , Utterback T , Weidman J , Nierman WC , Paulsen IT , Nelson KE , Tettelin H , O'Neill SL , Eisen JA
Ref : PLoS Biol , 2 :E69 , 2004
Abstract : The complete sequence of the 1,267,782 bp genome of Wolbachia pipientis wMel, an obligate intracellular bacteria of Drosophila melanogaster, has been determined. Wolbachia, which are found in a variety of invertebrate species, are of great interest due to their diverse interactions with different hosts, which range from many forms of reproductive parasitism to mutualistic symbioses. Analysis of the wMel genome, in particular phylogenomic comparisons with other intracellular bacteria, has revealed many insights into the biology and evolution of wMel and Wolbachia in general. For example, the wMel genome is unique among sequenced obligate intracellular species in both being highly streamlined and containing very high levels of repetitive DNA and mobile DNA elements. This observation, coupled with multiple evolutionary reconstructions, suggests that natural selection is somewhat inefficient in wMel, most likely owing to the occurrence of repeated population bottlenecks. Genome analysis predicts many metabolic differences with the closely related Rickettsia species, including the presence of intact glycolysis and purine synthesis, which may compensate for an inability to obtain ATP directly from its host, as Rickettsia can. Other discoveries include the apparent inability of wMel to synthesize lipopolysaccharide and the presence of the most genes encoding proteins with ankyrin repeat domains of any prokaryotic genome yet sequenced. Despite the ability of wMel to infect the germline of its host, we find no evidence for either recent lateral gene transfer between wMel and D. melanogaster or older transfers between Wolbachia and any host. Evolutionary analysis further supports the hypothesis that mitochondria share a common ancestor with the alpha-Proteobacteria, but shows little support for the grouping of mitochondria with species in the order Rickettsiales. With the availability of the complete genomes of both species and excellent genetic tools for the host, the wMel-D. melanogaster symbiosis is now an ideal system for studying the biology and evolution of Wolbachia infections.
ESTHER : Wu_2004_PLoS.Biol_2_E69
PubMedSearch : Wu_2004_PLoS.Biol_2_E69
PubMedID: 15024419
Gene_locus related to this paper: wolpm-q73gf0 , wolpm-q73gx7

Title : Genome of Geobacter sulfurreducens: metal reduction in subsurface environments - Methe_2003_Science_302_1967
Author(s) : Methe BA , Nelson KE , Eisen JA , Paulsen IT , Nelson W , Heidelberg JF , Wu D , Wu M , Ward N , Beanan MJ , Dodson RJ , Madupu R , Brinkac LM , Daugherty SC , DeBoy RT , Durkin AS , Gwinn M , Kolonay JF , Sullivan SA , Haft DH , Selengut J , Davidsen TM , Zafar N , White O , Tran B , Romero C , Forberger HA , Weidman J , Khouri H , Feldblyum TV , Utterback TR , Van Aken SE , Lovley DR , Fraser CM
Ref : Science , 302 :1967 , 2003
Abstract : The complete genome sequence of Geobacter sulfurreducens, a delta-proteobacterium, reveals unsuspected capabilities, including evidence of aerobic metabolism, one-carbon and complex carbon metabolism, motility, and chemotactic behavior. These characteristics, coupled with the possession of many two-component sensors and many c-type cytochromes, reveal an ability to create alternative, redundant, electron transport networks and offer insights into the process of metal ion reduction in subsurface environments. As well as playing roles in the global cycling of metals and carbon, this organism clearly has the potential for use in bioremediation of radioactive metals and in the generation of electricity.
ESTHER : Methe_2003_Science_302_1967
PubMedSearch : Methe_2003_Science_302_1967
PubMedID: 14671304
Gene_locus related to this paper: geosl-q74a54 , geosl-q74ac8 , geosl-q74eb1 , geosl-q747u4 , geosl-q747v8 , geosl-q749w4

Title : Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains - Fleischmann_2002_J.Bacteriol_184_5479
Author(s) : Fleischmann RD , Alland D , Eisen JA , Carpenter L , White O , Peterson J , Deboy R , Dodson R , Gwinn M , Haft D , Hickey E , Kolonay JF , Nelson WC , Umayam LA , Ermolaeva M , Salzberg SL , Delcher A , Utterback T , Weidman J , Khouri H , Gill J , Mikula A , Bishai W , Jacobs Jr WR, Jr. , Venter JC , Fraser CM
Ref : Journal of Bacteriology , 184 :5479 , 2002
Abstract : Virulence and immunity are poorly understood in Mycobacterium tuberculosis. We sequenced the complete genome of the M. tuberculosis clinical strain CDC1551 and performed a whole-genome comparison with the laboratory strain H37Rv in order to identify polymorphic sequences with potential relevance to disease pathogenesis, immunity, and evolution. We found large-sequence and single-nucleotide polymorphisms in numerous genes. Polymorphic loci included a phospholipase C, a membrane lipoprotein, members of an adenylate cyclase gene family, and members of the PE/PPE gene family, some of which have been implicated in virulence or the host immune response. Several gene families, including the PE/PPE gene family, also had significantly higher synonymous and nonsynonymous substitution frequencies compared to the genome as a whole. We tested a large sample of M. tuberculosis clinical isolates for a subset of the large-sequence and single-nucleotide polymorphisms and found widespread genetic variability at many of these loci. We performed phylogenetic and epidemiological analysis to investigate the evolutionary relationships among isolates and the origins of specific polymorphic loci. A number of these polymorphisms appear to have occurred multiple times as independent events, suggesting that these changes may be under selective pressure. Together, these results demonstrate that polymorphisms among M. tuberculosis strains are more extensive than initially anticipated, and genetic variation may have an important role in disease pathogenesis and immunity.
ESTHER : Fleischmann_2002_J.Bacteriol_184_5479
PubMedSearch : Fleischmann_2002_J.Bacteriol_184_5479
PubMedID: 12218036
Gene_locus related to this paper: myctu-a85a , myctu-a85b , myctu-a85c , myctu-bpoC , myctu-d5yk66 , myctu-ephA , myctu-ephB , myctu-ephc , myctu-ephd , myctu-ephE , myctu-ephF , myctu-hpx , myctu-linb , myctu-lipG , myctu-LPQP , myctu-MBTB , myctu-metx , myctu-mpt51 , myctu-MT3441 , myctu-p71654 , myctu-p95011 , myctu-PKS6 , myctu-PKS13 , myctu-ppe42 , myctu-ppe63 , myctu-Rv1430 , myctu-RV0045C , myctu-Rv0077c , myctu-Rv0151c , myctu-Rv0152c , myctu-Rv0159c , myctu-Rv0160c , myctu-rv0183 , myctu-Rv0217c , myctu-Rv0220 , myctu-Rv0272c , myctu-RV0293C , myctu-RV0421C , myctu-RV0457C , myctu-RV0519C , myctu-RV0774C , myctu-RV0782 , myctu-RV0840C , myctu-Rv1069c , myctu-Rv1076 , myctu-RV1123C , myctu-Rv1184c , myctu-Rv1190 , myctu-Rv1191 , myctu-RV1192 , myctu-RV1215C , myctu-Rv1399c , myctu-Rv1400c , myctu-RV1639C , myctu-RV1683 , myctu-RV1758 , myctu-Rv1800 , myctu-Rv1833c , myctu-Rv2045c , myctu-RV2054 , myctu-Rv2284 , myctu-RV2296 , myctu-Rv2385 , myctu-Rv2485c , myctu-RV2627C , myctu-RV2672 , myctu-RV2695 , myctu-RV2765 , myctu-RV2800 , myctu-RV2854 , myctu-Rv2970c , myctu-Rv3084 , myctu-Rv3097c , myctu-rv3177 , myctu-Rv3312c , myctu-RV3452 , myctu-Rv3487c , myctu-Rv3569c , myctu-Rv3591c , myctu-RV3724 , myctu-Rv3802c , myctu-Rv3822 , myctu-y0571 , myctu-y963 , myctu-Y1834 , myctu-y1835 , myctu-y2079 , myctu-Y2307 , myctu-yc88 , myctu-ym23 , myctu-ym24 , myctu-YR15 , myctu-yt28

Title : Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis - Heidelberg_2002_Nat.Biotechnol_20_1118
Author(s) : Heidelberg JF , Paulsen IT , Nelson KE , Gaidos EJ , Nelson WC , Read TD , Eisen JA , Seshadri R , Ward N , Methe B , Clayton RA , Meyer T , Tsapin A , Scott J , Beanan M , Brinkac L , Daugherty S , DeBoy RT , Dodson RJ , Durkin AS , Haft DH , Kolonay JF , Madupu R , Peterson JD , Umayam LA , White O , Wolf AM , Vamathevan J , Weidman J , Impraim M , Lee K , Berry K , Lee C , Mueller J , Khouri H , Gill J , Utterback TR , McDonald LA , Feldblyum TV , Smith HO , Venter JC , Nealson KH , Fraser CM
Ref : Nat Biotechnol , 20 :1118 , 2002
Abstract : Shewanella oneidensis is an important model organism for bioremediation studies because of its diverse respiratory capabilities, conferred in part by multicomponent, branched electron transport systems. Here we report the sequencing of the S. oneidensis genome, which consists of a 4,969,803-base pair circular chromosome with 4,758 predicted protein-encoding open reading frames (CDS) and a 161,613-base pair plasmid with 173 CDSs. We identified the first Shewanella lambda-like phage, providing a potential tool for further genome engineering. Genome analysis revealed 39 c-type cytochromes, including 32 previously unidentified in S. oneidensis, and a novel periplasmic [Fe] hydrogenase, which are integral members of the electron transport system. This genome sequence represents a critical step in the elucidation of the pathways for reduction (and bioremediation) of pollutants such as uranium (U) and chromium (Cr), and offers a starting point for defining this organism's complex electron transport systems and metal ion-reducing capabilities.
ESTHER : Heidelberg_2002_Nat.Biotechnol_20_1118
PubMedSearch : Heidelberg_2002_Nat.Biotechnol_20_1118
PubMedID: 12368813
Gene_locus related to this paper: sheon-BIOH , sheon-LYPA , sheon-PIP , sheon-PTRB , sheon-q8ej95 , sheon-SO0071 , sheon-SO0614 , sheon-SO0616 , sheon-SO0801 , sheon-SO0880 , sheoe-SO0967 , sheon-SO1006 , sheon-SO1224 , sheon-SO1310 , sheon-SO1534 , sheon-SO1539 , sheon-SO1686 , sheon-SO1743 , sheon-SO1976 , sheon-SO1999 , sheon-SO2024 , sheon-SO2047 , sheon-SO2055 , sheon-SO2223 , sheon-SO2333 , sheon-SO2473 , sheon-SO2582 , sheon-SO2753 , sheon-SO2934 , sheon-SO3025 , sheon-SO3900 , sheon-SO3990 , sheon-SO4252 , sheon-SO4400 , sheon-SO4537 , sheon-SO4543 , sheon-SO4574 , sheon-SO4618 , sheon-SO4650 , sheon-SOA0048 , shefn-SfSFGH , sheon-ym51

Title : Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39 - Read_2000_Nucleic.Acids.Res_28_1397
Author(s) : Read TD , Brunham RC , Shen C , Gill SR , Heidelberg JF , White O , Hickey EK , Peterson J , Utterback T , Berry K , Bass S , Linher K , Weidman J , Khouri H , Craven B , Bowman C , Dodson R , Gwinn M , Nelson W , Deboy R , Kolonay J , McClarty G , Salzberg SL , Eisen J , Fraser CM
Ref : Nucleic Acids Research , 28 :1397 , 2000
Abstract : The genome sequences of Chlamydia trachomatis mouse pneumonitis (MoPn) strain Nigg (1 069 412 nt) and Chlamydia pneumoniae strain AR39 (1 229 853 nt) were determined using a random shotgun strategy. The MoPn genome exhibited a general conservation of gene order and content with the previously sequenced C.trachomatis serovar D. Differences between C.trachomatis strains were focused on an approximately 50 kb 'plasticity zone' near the termination origins. In this region MoPn contained three copies of a novel gene encoding a >3000 amino acid toxin homologous to a predicted toxin from Escherichia coli O157:H7 but had apparently lost the tryptophan biosyntheis genes found in serovar D in this region. The C. pneumoniae AR39 chromosome was >99.9% identical to the previously sequenced C.pneumoniae CWL029 genome, however, comparative analysis identified an invertible DNA segment upstream of the uridine kinase gene which was in different orientations in the two genomes. AR39 also contained a novel 4524 nt circular single-stranded (ss)DNA bacteriophage, the first time a virus has been reported infecting C. pneumoniae. Although the chlamydial genomes were highly conserved, there were intriguing differences in key nucleotide salvage pathways: C.pneumoniae has a uridine kinase gene for dUTP production, MoPn has a uracil phosphororibosyl transferase, while C.trachomatis serovar D contains neither gene. Chromosomal comparison revealed that there had been multiple large inversion events since the species divergence of C.trachomatis and C.pneumoniae, apparently oriented around the axis of the origin of replication and the termination region. The striking synteny of the Chlamydia genomes and prevalence of tandemly duplicated genes are evidence of minimal chromosome rearrangement and foreign gene uptake, presumably owing to the ecological isolation of the obligate intracellular parasites. In the absence of genetic analysis, comparative genomics will continue to provide insight into the virulence mechanisms of these important human pathogens.
ESTHER : Read_2000_Nucleic.Acids.Res_28_1397
PubMedSearch : Read_2000_Nucleic.Acids.Res_28_1397
PubMedID: 10684935
Gene_locus related to this paper: chlmu-TC0345 , chlmu-TC0413 , chlmu-TC0426 , chlmu-TC0478 , chlpn-CPJ0152 , chlpn-CPJ0342 , chlpn-CPN0161 , chlpn-CPN0271 , chlpn-q9jrv1 , chlpn-q9js10 , chlpn-q9k1u7 , chlpn-q9z6x9

Title : Complete Genome Sequence of Treponema pallidum, the Syphilis Spirochete - Fraser_1998_Science_281_375
Author(s) : Fraser CM , Norris SJ , Weinstock GM , White O , Sutton GG , Dodson R , Gwinn M , Hickey EK , Clayton R , Ketchum KA , Sodergren E , Hardham JM , McLeod MP , Salzberg S , Peterson J , Khalak H , Richardson D , Howell JK , Chidambaram M , Utterback T , McDonald L , Artiach P , Bowman C , Cotton MD , Fujii C , Garland S , Hatch B , Horst K , Roberts K , Sandusky M , Weidman J , Smith HO , Venter JC
Ref : Science , 281 :375 , 1998
Abstract : The complete genome sequence of Treponema pallidum was determined and shown to be 1,138,006 base pairs containing 1041 predicted coding sequences (open reading frames). Systems for DNA replication, transcription, translation, and repair are intact, but catabolic and biosynthetic activities are minimized. The number of identifiable transporters is small, and no phosphoenolpyruvate:phosphotransferase carbohydrate transporters were found. Potential virulence factors include a family of 12 potential membrane proteins and several putative hemolysins. Comparison of the T. pallidum genome sequence with that of another pathogenic spirochete, Borrelia burgdorferi, the agent of Lyme disease, identified unique and common genes and substantiates the considerable diversity observed among pathogenic spirochetes.
ESTHER : Fraser_1998_Science_281_375
PubMedSearch : Fraser_1998_Science_281_375
PubMedID: 9665876
Gene_locus related to this paper: trepa-naptd , trepa-TP0902 , trepa-TP0952

Title : Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi - Fraser_1997_Nature_390_580
Author(s) : Fraser CM , Casjens S , Huang WM , Sutton GG , Clayton R , Lathigra R , White O , Ketchum KA , Dodson R , Hickey EK , Gwinn M , Dougherty B , Tomb JF , Fleischmann RD , Richardson D , Peterson J , Kerlavage AR , Quackenbush J , Salzberg S , Hanson M , van Vugt R , Palmer N , Adams MD , Gocayne J , Weidman J , Utterback T , Watthey L , McDonald L , Artiach P , Bowman C , Garland S , Fujii C , Cotton MD , Horst K , Roberts K , Hatch B , Smith HO , Venter JC
Ref : Nature , 390 :580 , 1997
Abstract : The genome of the bacterium Borrelia burgdorferi B31, the aetiologic agent of Lyme disease, contains a linear chromosome of 910,725 base pairs and at least 17 linear and circular plasmids with a combined size of more than 533,000 base pairs. The chromosome contains 853 genes encoding a basic set of proteins for DNA replication, transcription, translation, solute transport and energy metabolism, but, like Mycoplasma genitalium, it contains no genes for cellular biosynthetic reactions. Because B. burgdorferi and M. genitalium are distantly related eubacteria, we suggest that their limited metabolic capacities reflect convergent evolution by gene loss from more metabolically competent progenitors. Of 430 genes on 11 plasmids, most have no known biological function; 39% of plasmid genes are paralogues that form 47 gene families. The biological significance of the multiple plasmid-encoded genes is not clear, although they may be involved in antigenic variation or immune evasion.
ESTHER : Fraser_1997_Nature_390_580
PubMedSearch : Fraser_1997_Nature_390_580
PubMedID: 9403685
Gene_locus related to this paper: borbu-BB0646