Author Report for: Ishii YNo contact information in database for Ishii Y
Morita H, Toh H, Oshima K, Yoshizaki M, Kawanishi M, Nakaya K, Suzuki T, Miyauchi E, Ishii Y and Hattori M <2 more author(s)> Morita H, Toh H, Oshima K, Yoshizaki M, Kawanishi M, Nakaya K, Suzuki T, Miyauchi E, Ishii Y, Tanabe S, Murakami M, Hattori M (- 2) Ref: PLoS ONE, 6:e23184, 2011 : PubMed ESTHER: Morita_2011_PLoS.ONE_6_E23184 PubMedSearch: Morita 2011 PLoS.ONE 6 E23184 PubMedID: 21829716 Gene_locus related to this paper: lacgt-f9v7p0 Abstract Lactococcus garvieae causes fatal haemorrhagic septicaemia in fish such as yellowtail. The comparative analysis of genomes of a virulent strain Lg2 and a non-virulent strain ATCC 49156 of L. garvieae revealed that the two strains shared a high degree of sequence identity, but Lg2 had a 16.5-kb capsule gene cluster that is absent in ATCC 49156. The capsule gene cluster was composed of 15 genes, of which eight genes are highly conserved with those in exopolysaccharide biosynthesis gene cluster often found in Lactococcus lactis strains. Sequence analysis of the capsule gene cluster in the less virulent strain L. garvieae Lg2-S, Lg2-derived strain, showed that two conserved genes were disrupted by a single base pair deletion, respectively. These results strongly suggest that the capsule is crucial for virulence of Lg2. The capsule gene cluster of Lg2 may be a genomic island from several features such as the presence of insertion sequences flanked on both ends, different GC content from the chromosomal average, integration into the locus syntenic to other lactococcal genome sequences, and distribution in human gut microbiomes. The analysis also predicted other potential virulence factors such as haemolysin. The present study provides new insights into understanding of the virulence mechanisms of L. garvieae in fish. Title: Function of genes encoding acyl-CoA synthetase and enoyl-CoA hydratase for host-selective act-toxin biosynthesis in the tangerine pathotype of Alternaria alternata Miyamoto Y, Ishii Y, Honda A, Masunaka A, Tsuge T, Yamamoto M, Ohtani K, Fukumoto T, Gomi K and Akimitsu K <1 more author(s)> Miyamoto Y, Ishii Y, Honda A, Masunaka A, Tsuge T, Yamamoto M, Ohtani K, Fukumoto T, Gomi K, Peever TL, Akimitsu K (- 1) Ref: Phytopathology, 99:369, 2009 : PubMed ESTHER: Miyamoto_2009_Phytopathology_99_369 PubMedSearch: Miyamoto 2009 Phytopathology 99 369 PubMedID: 19271978 Gene_locus related to this paper: altal-actt2 Abstract The tangerine pathotype of Alternaria alternata produces host-selective ACT-toxin and causes Alternaria brown spot disease. Sequence analysis of a genomic cosmid clone identified a part of the ACTT gene cluster and implicated two genes, ACTT5 encoding an acyl-CoA synthetase and ACTT6 encoding an enoyl-CoA hydratase, in the biosynthesis of ACT-toxin. Genomic Southern blots demonstrated that both genes were present in tangerine pathotype isolates producing ACT-toxin and also in Japanese pear pathotype isolates producing AK-toxin and strawberry pathotype isolates producing AF-toxin. ACT-, AK-, and AF-toxins from these three pathotypes share a common 9,10-epoxy-8-hydroxy-9-methyl-decatrienoic acid moiety. Targeted gene disruption of two copies of ACTT5 significantly reduced ACT-toxin production and virulence. Targeted gene disruption of two copies of ACTT6 led to complete loss of ACT-toxin production and pathogenicity and a putative decatrienoic acid intermediate in ACT-toxin biosynthesis accumulated in mycelial mats. These results indicate that ACTT5 and ACTT6 are essential genes in ACT-toxin biosynthesis in the tangerine pathotype of A. alternata and both are required for full virulence of this fungus. Title: Functional protein-protein interaction of drug metabolizing enzymes Ishii Y, Takeda S, Yamada H, Oguri K Ref: Front Biosci, 10:887, 2005 : PubMed ESTHER: Ishii_2005_Front.Biosci_10_887 PubMedSearch: Ishii 2005 Front.Biosci 10 887 PubMedID: 15569627 Abstract Cytochrome P450 (P450, CYP), a major class of enzymes involved in Phase I drug metabolism, is expressed in the cellular endoplasmic reticulum together with other enzymes, such as microsomal epoxide hydrolase (mEH) and UDP-glucuronosyltransferase (UGT). In many cases, the metabolite produced by P450 is sequentially metabolized by other enzymes to increase its water solubility. It would be reasonable to assume that the metabolite produced by P450 is directly transferred to the other enzymes participating in its subsequent metabolism via protein-protein interactions for rapid metabolism. However, these steps have been considered to take place individually. Previously, we suggested that CYP1A1 specifically associates with mEH, UGTs and NADPH-P450 reductase. This observation strongly supports the view that there is functional cooperation between P450 and mEH/UGT to facilitate multistep drug metabolism. In recent years, accumulating evidence suggests the interaction between drug metabolizing enzymes and a change in enzymatic function by this interaction. In this review, we summarize the interaction between drug metabolizing enzymes and discuss its impact on their function. Title: Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D Matsuzaki M, Misumi O, Shin IT, Maruyama S, Takahara M, Miyagishima SY, Mori T, Nishida K, Yagisawa F and Kuroiwa T <31 more author(s)> Matsuzaki M, Misumi O, Shin IT, Maruyama S, Takahara M, Miyagishima SY, Mori T, Nishida K, Yagisawa F, Yoshida Y, Nishimura Y, Nakao S, Kobayashi T, Momoyama Y, Higashiyama T, Minoda A, Sano M, Nomoto H, Oishi K, Hayashi H, Ohta F, Nishizaka S, Haga S, Miura S, Morishita T, Kabeya Y, Terasawa K, Suzuki Y, Ishii Y, Asakawa S, Takano H, Ohta N, Kuroiwa H, Tanaka K, Shimizu N, Sugano S, Sato N, Nozaki H, Ogasawara N, Kohara Y, Kuroiwa T (- 31) Ref: Nature, 428:653, 2004 : PubMed ESTHER: Matsuzaki_2004_Nature_428_653 PubMedSearch: Matsuzaki 2004 Nature 428 653 PubMedID: 15071595 Gene_locus related to this paper: cyam1-m1vi61, cyam1-m1vhh9 Abstract Small, compact genomes of ultrasmall unicellular algae provide information on the basic and essential genes that support the lives of photosynthetic eukaryotes, including higher plants. Here we report the 16,520,305-base-pair sequence of the 20 chromosomes of the unicellular red alga Cyanidioschyzon merolae 10D as the first complete algal genome. We identified 5,331 genes in total, of which at least 86.3% were expressed. Unique characteristics of this genomic structure include: a lack of introns in all but 26 genes; only three copies of ribosomal DNA units that maintain the nucleolus; and two dynamin genes that are involved only in the division of mitochondria and plastids. The conserved mosaic origin of Calvin cycle enzymes in this red alga and in green plants supports the hypothesis of the existence of single primary plastid endosymbiosis. The lack of a myosin gene, in addition to the unexpressed actin gene, suggests a simpler system of cytokinesis. These results indicate that the C. merolae genome provides a model system with a simple gene composition for studying the origin, evolution and fundamental mechanisms of eukaryotic cells. Title: Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice Kikuchi S, Satoh K, Nagata T, Kawagashira N, Doi K, Kishimoto N, Yazaki J, Ishikawa M, Yamada H and Yasunishi A <64 more author(s)> Kikuchi S, Satoh K, Nagata T, Kawagashira N, Doi K, Kishimoto N, Yazaki J, Ishikawa M, Yamada H, Ooka H, Hotta I, Kojima K, Namiki T, Ohneda E, Yahagi W, Suzuki K, Li CJ, Ohtsuki K, Shishiki T, Otomo Y, Murakami K, Iida Y, Sugano S, Fujimura T, Suzuki Y, Tsunoda Y, Kurosaki T, Kodama T, Masuda H, Kobayashi M, Xie Q, Lu M, Narikawa R, Sugiyama A, Mizuno K, Yokomizo S, Niikura J, Ikeda R, Ishibiki J, Kawamata M, Yoshimura A, Miura J, Kusumegi T, Oka M, Ryu R, Ueda M, Matsubara K, Kawai J, Carninci P, Adachi J, Aizawa K, Arakawa T, Fukuda S, Hara A, Hashizume W, Hayatsu N, Imotani K, Ishii Y, Itoh M, Kagawa I, Kondo S, Konno H, Miyazaki A, Osato N, Ota Y, Saito R, Sasaki D, Sato K, Shibata K, Shinagawa A, Shiraki T, Yoshino M, Hayashizaki Y, Yasunishi A (- 64) Ref: Science, 301:376, 2003 : PubMed ESTHER: Kikuchi_2003_Science_301_376 PubMedSearch: Kikuchi 2003 Science 301 376 PubMedID: 12869764 Gene_locus related to this paper: orysa-Q852M6, orysa-Q8GSE8, orysa-Q9FYP7, orysa-Q5JLP6, orysa-Q8H5P5, orysa-Q7F1Y5, orysa-cbp3, orysa-Q6YSZ8, orysa-Q8S5X5, orysa-Q8LIG3, orysa-Q7F1B1, orysa-Q9FW17, orysa-Q337C3, orysa-Q84QZ6, orysa-Q84QY7, orysa-Q6ZDG5, orysa-Q658B2, orysa-Q8H3R3, orysa-Q5SNH3, orysa-q2qnj4, orysa-q2qyi1, orysa-Q4VWY7, orysa-q5smv5, orysa-q5z901, orysa-Q5ZBI5, orysa-q6atz0, orysa-q6i5q3, orysj-q6yse8, orysa-q6z8b1, orysa-q6z995, orysa-q7x7y5, orysa-q7xkj9, orysa-q7xr63, orysa-q7xsq2, orysa-q7xts6, orysa-Q8LQS5, orysa-Q8W3C6, orysa-q53m20, orysa-q67iz3, orysa-q67j02, orysa-q67j05, orysa-q67j09, orysa-q67j10, orysa-q67tv0, orysa-q67uz1, orysa-q69xr2, orysa-q69y21, orysa-q75hy2, orysa-q75i01, orysa-q688m8, orysa-q688m9, orysa-Q6H8G1, orysi-b8a7e7, orysi-b8bfe5, orysj-cgep, orysj-q0djj0, orysj-q0jaf0, orysj-q5jl22, orysj-q6h7q9, orysj-q6yvk6, orysj-q7f8x1, orysj-q10j20, orysj-q10ss2, orysj-q69uw6 Abstract We collected and completely sequenced 28,469 full-length complementary DNA clones from Oryza sativa L. ssp. japonica cv. Nipponbare. Through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). Mapping of the cDNA clones to genomic DNA revealed that there are 19,000 to 20,500 transcription units in the rice genome. Protein informatics analysis against the InterPro database revealed the existence of proteins presented in rice but not in Arabidopsis. Sixty-four percent of our cDNAs are homologous to Arabidopsis proteins. Title: Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs Okazaki Y, Furuno M, Kasukawa T, Adachi J, Bono H, Kondo S, Nikaido I, Osato N, Saito R and Hayashizaki Y <127 more author(s)> Okazaki Y, Furuno M, Kasukawa T, Adachi J, Bono H, Kondo S, Nikaido I, Osato N, Saito R, Suzuki H, Yamanaka I, Kiyosawa H, Yagi K, Tomaru Y, Hasegawa Y, Nogami A, Schonbach C, Gojobori T, Baldarelli R, Hill DP, Bult C, Hume DA, Quackenbush J, Schriml LM, Kanapin A, Matsuda H, Batalov S, Beisel KW, Blake JA, Bradt D, Brusic V, Chothia C, Corbani LE, Cousins S, Dalla E, Dragani TA, Fletcher CF, Forrest A, Frazer KS, Gaasterland T, Gariboldi M, Gissi C, Godzik A, Gough J, Grimmond S, Gustincich S, Hirokawa N, Jackson IJ, Jarvis ED, Kanai A, Kawaji H, Kawasawa Y, Kedzierski RM, King BL, Konagaya A, Kurochkin IV, Lee Y, Lenhard B, Lyons PA, Maglott DR, Maltais L, Marchionni L, McKenzie L, Miki H, Nagashima T, Numata K, Okido T, Pavan WJ, Pertea G, Pesole G, Petrovsky N, Pillai R, Pontius JU, Qi D, Ramachandran S, Ravasi T, Reed JC, Reed DJ, Reid J, Ring BZ, Ringwald M, Sandelin A, Schneider C, Semple CA, Setou M, Shimada K, Sultana R, Takenaka Y, Taylor MS, Teasdale RD, Tomita M, Verardo R, Wagner L, Wahlestedt C, Wang Y, Watanabe Y, Wells C, Wilming LG, Wynshaw-Boris A, Yanagisawa M, Yang I, Yang L, Yuan Z, Zavolan M, Zhu Y, Zimmer A, Carninci P, Hayatsu N, Hirozane-Kishikawa T, Konno H, Nakamura M, Sakazume N, Sato K, Shiraki T, Waki K, Kawai J, Aizawa K, Arakawa T, Fukuda S, Hara A, Hashizume W, Imotani K, Ishii Y, Itoh M, Kagawa I, Miyazaki A, Sakai K, Sasaki D, Shibata K, Shinagawa A, Yasunishi A, Yoshino M, Waterston R, Lander ES, Rogers J, Birney E, Hayashizaki Y (- 127) Ref: Nature, 420:563, 2002 : PubMed ESTHER: Okazaki_2002_Nature_420_563 PubMedSearch: Okazaki 2002 Nature 420 563 PubMedID: 12466851 Gene_locus related to this paper: mouse-1lipg, mouse-1llip, mouse-1plrp, mouse-3neur, mouse-ABH15, mouse-abhd4, mouse-abhd5, mouse-Abhd8, mouse-Abhd11, mouse-abhda, mouse-acot4, mouse-adcl4, mouse-AI607300, mouse-BAAT, mouse-bphl, mouse-C87498, mouse-Ldah, mouse-Ces1d, mouse-Ces2e, mouse-CMBL, mouse-DGLB, mouse-dpp9, mouse-ES10, mouse-F135A, mouse-FASN, mouse-hslip, mouse-hyes, mouse-Kansl3, mouse-LIPH, mouse-LIPK, mouse-lipli, mouse-LIPM, mouse-lypla1, mouse-lypla2, mouse-MEST, mouse-MGLL, mouse-ndr4, mouse-OVCA2, mouse-pafa, mouse-pcp, mouse-ppce, mouse-Ppgb, mouse-PPME1, mouse-q3uuq7, mouse-Q8BLF1, mouse-ACOT6, mouse-Q8C1A9, mouse-Q9DAI6, mouse-Q80UX8, mouse-Q8BGG9, mouse-Q8C167, mouse-rbbp9, mouse-SERHL, mouse-tssp Abstract Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the mouse, and to ensure that each is represented in a physical collection of clones. Here we report the manual annotation of 60,770 full-length mouse complementary DNA sequences. These are clustered into 33,409 'transcriptional units', contributing 90.1% of a newly established mouse transcriptome database. Of these transcriptional units, 4,258 are new protein-coding and 11,665 are new non-coding messages, indicating that non-coding RNA is a major component of the transcriptome. 41% of all transcriptional units showed evidence of alternative splicing. In protein-coding transcripts, 79% of splice variations altered the protein product. Whole-transcriptome analyses resulted in the identification of 2,431 sense-antisense pairs. The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics. Title: Functional annotation of a full-length Arabidopsis cDNA collection Seki M, Narusaka M, Kamiya A, Ishida J, Satou M, Sakurai T, Nakajima M, Enju A, Akiyama K and Shinozaki K <10 more author(s)> Seki M, Narusaka M, Kamiya A, Ishida J, Satou M, Sakurai T, Nakajima M, Enju A, Akiyama K, Oono Y, Muramatsu M, Hayashizaki Y, Kawai J, Carninci P, Itoh M, Ishii Y, Arakawa T, Shibata K, Shinagawa A, Shinozaki K (- 10) Ref: Science, 296:141, 2002 : PubMed ESTHER: Seki_2002_Science_296_141 PubMedSearch: Seki 2002 Science 296 141 PubMedID: 11910074 Gene_locus related to this paper: arath-CXE15 Abstract Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins. Title: Functional annotation of a full-length mouse cDNA collection Kawai J, Shinagawa A, Shibata K, Yoshino M, Itoh M, Ishii Y, Arakawa T, Hara A, Fukunishi Y and Hayashizaki Y <86 more author(s)> Kawai J, Shinagawa A, Shibata K, Yoshino M, Itoh M, Ishii Y, Arakawa T, Hara A, Fukunishi Y, Konno H, Adachi J, Fukuda S, Aizawa K, Izawa M, Nishi K, Kiyosawa H, Kondo S, Yamanaka I, Saito T, Okazaki Y, Gojobori T, Bono H, Kasukawa T, Saito R, Kadota K, Matsuda H, Ashburner M, Batalov S, Casavant T, Fleischmann W, Gaasterland T, Gissi C, King B, Kochiwa H, Kuehl P, Lewis S, Matsuo Y, Nikaido I, Pesole G, Quackenbush J, Schriml LM, Staubli F, Suzuki R, Tomita M, Wagner L, Washio T, Sakai K, Okido T, Furuno M, Aono H, Baldarelli R, Barsh G, Blake J, Boffelli D, Bojunga N, Carninci P, de Bonaldo MF, Brownstein MJ, Bult C, Fletcher C, Fujita M, Gariboldi M, Gustincich S, Hill D, Hofmann M, Hume DA, Kamiya M, Lee NH, Lyons P, Marchionni L, Mashima J, Mazzarelli J, Mombaerts P, Nordone P, Ring B, Ringwald M, Rodriguez I, Sakamoto N, Sasaki H, Sato K, Schonbach C, Seya T, Shibata Y, Storch KF, Suzuki H, Toyo-oka K, Wang KH, Weitz C, Whittaker C, Wilming L, Wynshaw-Boris A, Yoshida K, Hasegawa Y, Kawaji H, Kohtsuki S, Hayashizaki Y (- 86) Ref: Nature, 409:685, 2001 : PubMed ESTHER: Kawai_2001_Nature_409_685 PubMedSearch: Kawai 2001 Nature 409 685 PubMedID: 11217851 Gene_locus related to this paper: mouse-1lipg, mouse-1plip, mouse-1plrp, mouse-ABH15, mouse-abhd5, mouse-ABHD6, mouse-Abhd8, mouse-aryla, mouse-bphl, mouse-cauxin, mouse-Ces1g, mouse-CPMac, mouse-dpp8, mouse-EPHX1, mouse-ES10, mouse-hslip, mouse-hyes, mouse-ABHD2, mouse-lcat, mouse-lipli, mouse-LIPN, mouse-lypla1, mouse-lypla2, mouse-OVCA2, mouse-pafa, mouse-pcp, mouse-Ppgb, mouse-PPME1, mouse-ppt, mouse-q3uuq7, mouse-Q9DAI6, mouse-Q80UX8, mouse-RISC, mouse-SERHL, mouse-SPG21, mouse-Tex30 Abstract The RIKEN Mouse Gene Encyclopaedia Project, a systematic approach to determining the full coding potential of the mouse genome, involves collection and sequencing of full-length complementary DNAs and physical mapping of the corresponding genes to the mouse genome. We organized an international functional annotation meeting (FANTOM) to annotate the first 21,076 cDNAs to be analysed in this project. Here we describe the first RIKEN clone collection, which is one of the largest described for any organism. Analysis of these cDNAs extends known gene families and identifies new ones. Title: Plasma endotoxin and serum cytokine levels in patients with alcoholic hepatitis: relation to severity of liver disturbance Fujimoto M, Uemura M, Nakatani Y, Tsujita S, Hoppo K, Tamagawa T, Kitano H, Kikukawa M, Ann T and Fukui H <10 more author(s)> Fujimoto M, Uemura M, Nakatani Y, Tsujita S, Hoppo K, Tamagawa T, Kitano H, Kikukawa M, Ann T, Ishii Y, Kojima H, Sakurai S, Tanaka R, Namisaki T, Noguchi R, Higashino T, Kikuchi E, Nishimura K, Takaya A, Fukui H (- 10) Ref: Alcohol Clin Exp Res, 24:48S, 2000 : PubMed ESTHER: Fujimoto_2000_Alcohol.Clin.Exp.Res_24_48S PubMedSearch: Fujimoto 2000 Alcohol.Clin.Exp.Res 24 48S PubMedID: 10803780 Abstract BACKGROUND: Endotoxin plays an important role in the initiation and aggravation of alcoholic liver disease. In this study, we evaluated plasma endotoxin levels and serum concentrations of cytokines and lipopolysaccharide binding protein (LBP) during the acute and recovery phase of patients with alcoholic hepatitis; we also explored the prognostic factors associated with a fatal outcome. Title: RIKEN integrated sequence analysis (RISA) system--384-format sequencing pipeline with 384 multicapillary sequencer Shibata K, Itoh M, Aizawa K, Nagaoka S, Sasaki N, Carninci P, Konno H, Akiyama J, Nishi K and Togawa Y <15 more author(s)> Shibata K, Itoh M, Aizawa K, Nagaoka S, Sasaki N, Carninci P, Konno H, Akiyama J, Nishi K, Kitsunai T, Tashiro H, Sumi N, Ishii Y, Nakamura S, Hazama M, Nishine T, Harada A, Yamamoto R, Matsumoto H, Sakaguchi S, Ikegami T, Kashiwagi K, Fujiwake S, Inoue K, Togawa Y (- 15) Ref: Genome Res, 10:1757, 2000 : PubMed ESTHER: Shibata_2000_Genome.Res_10_1757 PubMedSearch: Shibata 2000 Genome.Res 10 1757 PubMedID: 11076861 Gene_locus related to this paper: mouse-1plrp, mouse-abhd5, mouse-Abhd8, mouse-cauxin, mouse-dpp8, mouse-hslip, mouse-lipli, mouse-LIPN, mouse-Ppgb, mouse-q3uuq7, mouse-Q9DAI6 Abstract The RIKEN high-throughput 384-format sequencing pipeline (RISA system) including a 384-multicapillary sequencer (the so-called RISA sequencer) was developed for the RIKEN mouse encyclopedia project. The RISA system consists of colony picking, template preparation, sequencing reaction, and the sequencing process. A novel high-throughput 384-format capillary sequencer system (RISA sequencer system) was developed for the sequencing process. This system consists of a 384-multicapillary auto sequencer (RISA sequencer), a 384-multicapillary array assembler (CAS), and a 384-multicapillary casting device. The RISA sequencer can simultaneously analyze 384 independent sequencing products. The optical system is a scanning system chosen after careful comparison with an image detection system for the simultaneous detection of the 384-capillary array. This scanning system can be used with any fluorescent-labeled sequencing reaction (chain termination reaction), including transcriptional sequencing based on RNA polymerase, which was originally developed by us, and cycle sequencing based on thermostable DNA polymerase. For long-read sequencing, 380 out of 384 sequences (99.2%) were successfully analyzed and the average read length, with more than 99% accuracy, was 654.4 bp. A single RISA sequencer can analyze 216 kb with >99% accuracy in 2.7 h (90 kb/h). For short-read sequencing to cluster the 3' end and 5' end sequencing by reading 350 bp, 384 samples can be analyzed in 1.5 h. We have also developed a RISA inoculator, RISA filtrator and densitometer, RISA plasmid preparator which can handle throughput of 40,000 samples in 17.5 h, and a high-throughput RISA thermal cycler which has four 384-well sites. The combination of these technologies allowed us to construct the RISA system consisting of 16 RISA sequencers, which can process 50,000 DNA samples per day. One haploid genome shotgun sequence of a higher organism, such as human, mouse, rat, domestic animals, and plants, can be revealed by seven RISA systems within one month. Title: Interaction between cytochrome P450 and other drug-metabolizing enzymes: evidence for an association of CYP1A1 with microsomal epoxide hydrolase and UDP-glucuronosyltransferase Taura KI, Yamada H, Hagino Y, Ishii Y, Mori MA, Oguri K Ref: Biochemical & Biophysical Research Communications, 273:1048, 2000 : PubMed ESTHER: Taura_2000_Biochem.Biophys.Res.Commun_273_1048 PubMedSearch: Taura 2000 Biochem.Biophys.Res.Commun 273 1048 PubMedID: 10891369 Abstract Protein-protein interactions between cytochrome P450 (P450) and other drug-metabolizing enzymes were studied by affinity chromatography using CYP1A1-, glycine-, and bovine serum albumin (BSA)-conjugated Sepharose 4B columns. Sodium cholate-solubilized microsomes from phenobarbital-treated rat liver were applied to the columns and the material eluted with buffer containing NaCl was analyzed by immunoblotting. Microsomal epoxide hydrolase (mEH) and UDP-glucuronosyltransferases (UGTs), as well as NADPH-P450 reductase, were efficiently trapped by the CYP1A1 column. Glycine and BSA columns exhibited no ability to retain these proteins. Protein disulfide isomerase and calnexin, non-drug-metabolizing enzymes expressed in the endoplasmic reticulum, were unable to associate with the CYP1A1 column. These results suggest that CYP1A1 interacts with mEH and UGT to facilitate a series of multistep drug metabolic conversions. Title: Effect of NIK-247 on basal concentrations of extracellular acetylcholine in the cerebral cortex of conscious, freely moving rats Ishii Y, Kojima J, Ikeda N, Kawashima K Ref: Japanese Journal of Pharmacology, 66:289, 1994 : PubMed ESTHER: Ishii_1994_Jpn.J.Pharmacol_66_289 PubMedSearch: Ishii 1994 Jpn.J.Pharmacol 66 289 PubMedID: 7869615 Inhibitor(s) related to this paper: NIK-247 Abstract We studied the effect of orally administered NIK-247 (9-amino-2,3,5,6,7,8-hexahydro-1H-cyclopenta[b]quinoline monohydrochloride monohydrate) on basal extracellular acetylcholine (ACh) concentrations in the rat cerebral cortex using microdialysis without the addition of cholinesterase inhibitor to the perfusion fluid and radioimmunoassay for ACh. In addition, the effect of oral administration of NIK-247 on acetylcholinesterase (AChE) activity in rat cerebral cortex was determined. The mean basal ACh content in the perfusate from the cerebral cortex of freely moving rats was 123.2 +/- 21.8 fmol/30 min (n = 7). NIK-247 (2.5-10.0 mg/kg, p.o.) increased the ACh content of the perfusate in a dose-dependent manner. NIK-247 at 10 mg/kg significantly increased the ACh content in the perfusate from 0.5 to 2.5 hr after administration, and the maximum increase was attained at 1 hr after administration. 9-Amino-1,2,3,4-tetrahydroacridine (5 mg/kg, p.o.) and physostigmine (0.5 mg/kg, i.p.) significantly increased the ACh content in the perfusate from 1 to 2 hr and from 0.5 to 1.5 hr after administration, respectively. AChE activities in the cerebral cortex were about 32% and 12% below the control value at 1 hr and 3 hr after administration of NIK-247 at 10 mg/kg, respectively. These findings demonstrate that NIK-247 increases extracellular ACh concentration and inhibits AChE activity in the cerebral cortex after oral administration, and they suggest that NIK-247 facilitates central cholinergic transmission. Title: Evaluation of a cholinomimetic drug, 9-amino-2,3,5,6,7,8-hexahydro-1H-cyclopenta [b] quinoline (NIK-247), as an enhancer of endogenous efflux of acetylcholine from brain slices Ishii Y, Sumi T Ref: Neuropharmacology, 31:61, 1992 : PubMed ESTHER: Ishii_1992_Neuropharmacol_31_61 PubMedSearch: Ishii 1992 Neuropharmacol 31 61 PubMedID: 1542404 Inhibitor(s) related to this paper: NIK-247 Abstract Basal and high K(+)-stimulated efflux of endogenous ACh from slices of brain was measured to evaluate the cholinomimetic effect of 9-amino-2,3,5,6,7,8-hexahydro-1H-cyclopenta[b] quinoline monohydrate HCl (NIK-247) on the central nervous system. The drug NIK-247 dose-dependently accelerated the efflux of ACh from slices of striatum. The maximum increase produced by 1.0 x 10(-4) M of NIK-247 was 329% in basal and 1332% in 30 mM K(+)-stimulated efflux. This drug was nearly twice as potent as THA (9-amino-1,2,3,4-tetrahydroacridine HCl) but had the same potency as physostigmine, in enhancing basal efflux, although there was no significant difference between the efficacy of these drugs in enhancing the K(+)-stimulated efflux. Both basal and 50 mM K(+)-stimulated efflux of ACh were increased by NIK-247, not only from the striatum but also from slices of frontal cortex and hippocampus. The activity was more effective in the striatum than in other tissues, and more effective on K(+)-stimulated than on basal efflux, regardless of the region of the brain. These effects of NIK-247 may be a result mainly of its inhibition of cholinesterase and its other biological characteristics, such as K+ channel blockade, capable of modulating release of ACh, may not be of major importance. Title: Role of adrenergic neuronal activity in the yawning induced by tacrine and NIK-247 in rats Kimura H, Yamada K, Nagashima M, Matsumoto S, Ishii Y, Yoshida S, Fujii K, Furukawa T Ref: Pharmacol Biochem Behav, 43:985, 1992 : PubMed ESTHER: Kimura_1992_Pharmacol.Biochem.Behav_43_985 PubMedSearch: Kimura 1992 Pharmacol.Biochem.Behav 43 985 PubMedID: 1361995 Inhibitor(s) related to this paper: NIK-247 Abstract The present experiments were performed to investigate the potential role of central adrenergic neurons in regulating occurrence of yawning in rats. Intraperitoneal injection of tacrine (THA) or 9-amino-2,3,5,6,7,8-hexahydro-1H-cyclopenta(b)-quinoline monohydrate HCl (NIK-247), cholinesterase inhibitors, induced yawning, which was markedly increased by pretreatment with the beta-adrenoceptor antagonist, pindolol. The yawning evoked by tacrine or NIK-247 given alone or in combination with pindolol was inhibited by pretreatment with scopolamine but not by mecamylamine or spiperone. Treatment with tacrine or NIK-247 increased acetylcholine content of the striatum, but this effect was not enhanced by pindolol, which per se did not affect basal acetylcholine content. Moreover, pretreatment with the central adrenaline synthesis inhibitors, (+-)-2,3-dichloro-alpha-methylbenzylamine HCl (LY-78335) and 2-cyclooctyl-2-hydroxyethylamine HCl (UK-1187A), increased tacrine-induced yawning. Subcutaneous injection of talipexole (B-HT 920), a dopamine D2 receptor agonist, evoked yawning, which was also increased by pindolol, LY-78335, and UK-1187A. These receptors antagonists and synthesis inhibitors per se did not cause yawning responses. The results suggest that the beta-adrenoceptor blockade and the inhibition of adrenaline synthesis facilitate the occurrence of yawning induced by cholinergic and dopaminergic agonists, and thus the central adrenergic neuronal systems may be implicated in the regulation of yawning responses. Title: Effect of 9-amino-2,3,5,6,7,8-hexahydro-1H-cyclopenta-(b)-quinoline monohydrate hydrochloride (NIK-247) on cholinergic enzyme activity in rats Shibanoki S, Ishii Y, Kubo T, Kogure M, Asai S, Ishikawa K Ref: Pharmacol Biochem Behav, 39:499, 1991 : PubMed ESTHER: Shibanoki_1991_Pharmacol.Biochem.Behav_39_499 PubMedSearch: Shibanoki 1991 Pharmacol.Biochem.Behav 39 499 PubMedID: 1946590 Inhibitor(s) related to this paper: NIK-247 Abstract An in vitro comparison demonstrated that the concentration of NIK-247 that inhibited cholinesterase (ChE) activities to half the normal level (ID50) was 1.3 x 10(-6) M. This value was higher than those for both physostigmine (PHY; 1.2 x 10(-7) M) and tetrahydroaminoacridine (THA; 3.6 x 10(-7) M), which are used as cholinesterase inhibitors in the treatment of cholinergic deficits. Neither NIK-247 nor THA affected the activity of choline acetyltransferase (ChAT). These inhibitions of ChE by NIK-247 and PHY lasted for 2 h, while that by THA lasted for over 4 h. In the effects of NIK-247 and PHY, the concentrations of intrastriatal acetylcholine (ACh) were changed in relation to the inhibition of the ChE activity. However, THA caused a transient increase in the ACh level lasting for only 2 h instead of inhibiting the enzyme activity for over 4 h. These findings suggest that NIK-247 is a drug with a similar profile in its effect on cholinergic neurons to PHY, the prototype drug among ChE inhibitors. The data indicate that NIK-247 may be useful as a drug for the treatment of central as well as peripheral deficits of the cholinergic mechanism. | |||||||
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