Family: BioH

Block: X

Parent Family: Abhydrolase_6


Pimelyl-[acyl-carrier protein] methyl ester esterase: BioH family previously included in AlphaBeta_hydrolase family. The structure of ecoli-bioh by Sanishvili et al pdb:1M33 allowed description of function of this family. This CoA-binding enzyme is required for the production of pimeloyl-coenzyme A, the substrate of the BioF protein early in the biosynthesis of biotin. Its exact function is unknown. Members of this family are restricted to the proteobacteria. BioH shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). It also displays a weak thioesterase activity. Biotin synthesis necessitate the fatty acid synthetic pathway: Malonyl-thioester is methylated by BioC, which allows recognition of this atypical substrate by the fatty acid synthetic enzymes. The malonyl-thioester methyl ester enters fatty acid synthesis as the primer and undergoes two reiterations of the fatty acid elongation cycle to give pimeloyl-acyl carrier protein (ACP) methyl ester, which is hydrolyzed to pimeloyl-ACP and methanol by BioH. In Pseudomonas aeruginosa the bioH gene is within a biotin synthesis operon and its transcription is coregulated with the other biotin operon genes. In Escherichia coli the gene is not located within a biotin synthetic operon and its transcription is not coregulated with the other biotin synthesis genes (Cao et al. 2017). In Francisella BioJ is the enzyme of the biotin biosynthesis pathway that determines the chain length of the biotin valeryl side-chain. It is the functionnal equivalent of BioH although it shows low homology to BioH (belongs to hormone sensitive lipase family)( Feng et al. 2014). In Haemophilus influenzae it is another family Duf_452 which plays the role (Shi et al. 2016). In Helicobacter it is another alpha/beta hydrolase BioV (Bi et al. 2016)


Interpro : IPR010076 BioH Pimelyl-[acyl-carrier protein] methyl ester esterase


Pdoc : No Pdoc

Pfam : No Pfam

Prints : No Print

EC Number : No EC Number


Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour
Alignment with MAFFT No colour
Dendrogram The dnd file

Structures (4)

Genes Proteins in BioH family (220)

Fragments of genes in BioH family (2)

Structures in BioH family (4)

Substrates in BioH family (7)

Inhibitors in BioH family (2)

References (7)

Title : Expression and Activity of the BioH Esterase of Biotin Synthesis is Independent of Genome Context - Cao_2017_Sci.Rep_7_2141
Author(s) : Cao X , Zhu L , Hu Z , Cronan JE
Ref : Sci Rep , 7 :2141 , 2017
PubMedID: 28526858
Gene_locus related to this paper: fratt-q5nga8 , pseae-PA0502

Title : A Biotin Biosynthesis Gene Restricted to Helicobacter - Bi_2016_Sci.Rep_6_21162
Author(s) : Bi H , Zhu L , Jia J , Cronan JE
Ref : Sci Rep , 6 :21162 , 2016
PubMedID: 26868423
Gene_locus related to this paper: 9gamm-BioJ , helpy-o25061

Title : An Atypical alpha\/beta-Hydrolase Fold Revealed in the Crystal Structure of Pimeloyl-Acyl Carrier Protein Methyl Esterase BioG from Haemophilus influenzae - Shi_2016_Biochemistry_55_6705
Author(s) : Shi J , Cao X , Chen Y , Cronan JE , Guo Z
Ref : Biochemistry , 55 :6705 , 2016
PubMedID: 27933801
Gene_locus related to this paper: haein-y1552

Title : A Francisella virulence factor catalyses an essential reaction of biotin synthesis - Feng_2014_Mol.Microbiol_91_300
Author(s) : Feng Y , Napier BA , Manandhar M , Henke SK , Weiss DS , Cronan JE
Ref : Molecular Microbiology , 91 :300 , 2014
PubMedID: 24313380
Gene_locus related to this paper: 9gamm-BioJ , fratt-q5nga8

Title : Biotin synthesis begins by hijacking the fatty acid synthetic pathway. - Lin_2010_Nat.Chem.Biol_6_682
Author(s) : Lin S , Hanson RE , Cronan JE
Ref : Nat Chemical Biology , 6 :682 , 2010
PubMedID: 20693992
Gene_locus related to this paper: 9gamm-BioJ , ecoli-bioh

Title : Integrating structure, bioinformatics, and enzymology to discover function: BioH, a new carboxylesterase from Escherichia coli - Sanishvili_2003_J.Biol.Chem_278_26039
Author(s) : Sanishvili R , Yakunin AF , Laskowski RA , Skarina T , Evdokimova E , Doherty-Kirby A , Lajoie GA , Thornton JM , Arrowsmith CH , Savchenko A , Joachimiak A , Edwards AM
Ref : Journal of Biological Chemistry , 278 :26039 , 2003
PubMedID: 12732651
Gene_locus related to this paper: ecoli-bioh

Title : Purification and characterisation of the BIOH protein from the biotin biosynthetic pathway - Tomczyk_2002_FEBS.Lett_513_299
Author(s) : Tomczyk NH , Nettleship JE , Baxter RL , Crichton HJ , Webster SP , Campopiano DJ
Ref : FEBS Letters , 513 :299 , 2002
PubMedID: 11904168
Gene_locus related to this paper: ecoli-bioh , neime-NMA2216