LaButti K

References (26)

Title : Genome expansion by allopolyploidization in the fungal strain Coniochaeta 2T2.1 and its exceptional lignocellulolytic machinery - Mondo_2019_Biotechnol.Biofuels_12_229
Author(s) : Mondo SJ , Jimenez DJ , Hector RE , Lipzen A , Yan M , LaButti K , Barry K , van Elsas JD , Grigoriev IV , Nichols NN
Ref : Biotechnol Biofuels , 12 :229 , 2019
Abstract : BACKGROUND: Particular species of the genus Coniochaeta (Sordariomycetes) exhibit great potential for bioabatement of furanic compounds and have been identified as an underexplored source of novel lignocellulolytic enzymes, especially Coniochaeta ligniaria. However, there is a lack of information about their genomic features and metabolic capabilities. Here, we report the first in-depth genome/transcriptome survey of a Coniochaeta species (strain 2T2.1). RESULTS: The genome of Coniochaeta sp. strain 2T2.1 has a size of 74.53 Mbp and contains 24,735 protein-encoding genes. Interestingly, we detected a genome expansion event, resulting ~ 98% of the assembly being duplicated with 91.9% average nucleotide identity between the duplicated regions. The lack of gene loss, as well as the high divergence and strong genome-wide signatures of purifying selection between copies indicates that this is likely a recent duplication, which arose through hybridization between two related Coniochaeta-like species (allopolyploidization). Phylogenomic analysis revealed that 2T2.1 is related Coniochaeta sp. PMI546 and Lecythophora sp. AK0013, which both occur endophytically. Based on carbohydrate-active enzyme (CAZy) annotation, we observed that even after in silico removal of its duplicated content, the 2T2.1 genome contains exceptional lignocellulolytic machinery. Moreover, transcriptomic data reveal the overexpression of proteins affiliated to CAZy families GH11, GH10 (endoxylanases), CE5, CE1 (xylan esterases), GH62, GH51 (alpha-l-arabinofuranosidases), GH12, GH7 (cellulases), and AA9 (lytic polysaccharide monoxygenases) when the fungus was grown on wheat straw compared with glucose as the sole carbon source. CONCLUSIONS: We provide data that suggest that a recent hybridization between the genomes of related species may have given rise to Coniochaeta sp. 2T2.1. Moreover, our results reveal that the degradation of arabinoxylan, xyloglucan and cellulose are key metabolic processes in strain 2T2.1 growing on wheat straw. Different genes for key lignocellulolytic enzymes were identified, which can be starting points for production, characterization and/or supplementation of enzyme cocktails used in saccharification of agricultural residues. Our findings represent first steps that enable a better understanding of the reticulate evolution and "eco-enzymology" of lignocellulolytic Coniochaeta species.
ESTHER : Mondo_2019_Biotechnol.Biofuels_12_229
PubMedSearch : Mondo_2019_Biotechnol.Biofuels_12_229
PubMedID: 31572496
Gene_locus related to this paper: 9pezi-a0a5n5p0y9 , 9pezi-a0a5n5pg71 , 9pezi-a0a5n5ld08

Title : Comparative genomics and transcriptomics depict ericoid mycorrhizal fungi as versatile saprotrophs and plant mutualists - Martino_2018_New.Phytol_217_1213
Author(s) : Martino E , Morin E , Grelet GA , Kuo A , Kohler A , Daghino S , Barry KW , Cichocki N , Clum A , Dockter RB , Hainaut M , Kuo RC , LaButti K , Lindahl BD , Lindquist EA , Lipzen A , Khouja HR , Magnuson J , Murat C , Ohm RA , Singer SW , Spatafora JW , Wang M , Veneault-Fourrey C , Henrissat B , Grigoriev IV , Martin FM , Perotto S
Ref : New Phytol , 217 :1213 , 2018
Abstract : Some soil fungi in the Leotiomycetes form ericoid mycorrhizal (ERM) symbioses with Ericaceae. In the harsh habitats in which they occur, ERM plant survival relies on nutrient mobilization from soil organic matter (SOM) by their fungal partners. The characterization of the fungal genetic machinery underpinning both the symbiotic lifestyle and SOM degradation is needed to understand ERM symbiosis functioning and evolution, and its impact on soil carbon (C) turnover. We sequenced the genomes of the ERM fungi Meliniomyces bicolor, M. variabilis, Oidiodendron maius and Rhizoscyphus ericae, and compared their gene repertoires with those of fungi with different lifestyles (ecto- and orchid mycorrhiza, endophytes, saprotrophs, pathogens). We also identified fungal transcripts induced in symbiosis. The ERM fungal gene contents for polysaccharide-degrading enzymes, lipases, proteases and enzymes involved in secondary metabolism are closer to those of saprotrophs and pathogens than to those of ectomycorrhizal symbionts. The fungal genes most highly upregulated in symbiosis are those coding for fungal and plant cell wall-degrading enzymes (CWDEs), lipases, proteases, transporters and mycorrhiza-induced small secreted proteins (MiSSPs). The ERM fungal gene repertoire reveals a capacity for a dual saprotrophic and biotrophic lifestyle. This may reflect an incomplete transition from saprotrophy to the mycorrhizal habit, or a versatile life strategy similar to fungal endophytes.
ESTHER : Martino_2018_New.Phytol_217_1213
PubMedSearch : Martino_2018_New.Phytol_217_1213
PubMedID: 29315638
Gene_locus related to this paper: amore-a0a2t3axk4 , amore-a0a2t3avs4 , amore-a0a2t3ay04 , amore-a0a2t3aph0

Title : Genome-Wide Analysis of Corynespora cassiicola Leaf Fall Disease Putative Effectors - Lopez_2018_Front.Microbiol_9_276
Author(s) : Lopez D , Ribeiro S , Label P , Fumanal B , Venisse JS , Kohler A , de Oliveira RR , LaButti K , Lipzen A , Lail K , Bauer D , Ohm RA , Barry KW , Spatafora J , Grigoriev IV , Martin FM , Pujade-Renaud V
Ref : Front Microbiol , 9 :276 , 2018
Abstract : Corynespora cassiicola is an Ascomycetes fungus with a broad host range and diverse life styles. Mostly known as a necrotrophic plant pathogen, it has also been associated with rare cases of human infection. In the rubber tree, this fungus causes the Corynespora leaf fall (CLF) disease, which increasingly affects natural rubber production in Asia and Africa. It has also been found as an endophyte in South American rubber plantations where no CLF outbreak has yet occurred. The C. cassiicola species is genetically highly diverse, but no clear relationship has been evidenced between phylogenetic lineage and pathogenicity. Cassiicolin, a small glycosylated secreted protein effector, is thought to be involved in the necrotrophic interaction with the rubber tree but some virulent C. cassiicola isolates do not have a cassiicolin gene. This study set out to identify other putative effectors involved in CLF. The genome of a highly virulent C. cassiicola isolate from the rubber tree (CCP) was sequenced and assembled. In silico prediction revealed 2870 putative effectors, comprising CAZymes, lipases, peptidases, secreted proteins and enzymes associated with secondary metabolism. Comparison with the genomes of 44 other fungal species, focusing on effector content, revealed a striking proximity with phylogenetically unrelated species (Colletotrichum acutatum, Colletotrichum gloesporioides, Fusarium oxysporum, nectria hematococca, and Botrosphaeria dothidea) sharing life style plasticity and broad host range. Candidate effectors involved in the compatible interaction with the rubber tree were identified by transcriptomic analysis. Differentially expressed genes included 92 putative effectors, among which cassiicolin and two other secreted singleton proteins. Finally, the genomes of 35 C. cassiicola isolates representing the genetic diversity of the species were sequenced and assembled, and putative effectors identified. At the intraspecific level, effector-based classification was found to be highly consistent with the phylogenomic trees. Identification of lineage-specific effectors is a key step toward understanding C. cassiicola virulence and host specialization mechanisms.
ESTHER : Lopez_2018_Front.Microbiol_9_276
PubMedSearch : Lopez_2018_Front.Microbiol_9_276
PubMedID: 29551995
Gene_locus related to this paper: corcc-a0a2t2nss3 , corcc-a0a2t2n5c6 , corcc-a0a2t2n3a1 , corcc-a0a2t2p617 , corcc-a0a2t2nt04 , corcc-a0a2t2n262

Title : Linking secondary metabolites to gene clusters through genome sequencing of six diverse Aspergillus species - Kjaerbolling_2018_Proc.Natl.Acad.Sci.U.S.A_115_E753
Author(s) : Kjaerbolling I , Vesth TC , Frisvad JC , Nybo JL , Theobald S , Kuo A , Bowyer P , Matsuda Y , Mondo S , Lyhne EK , Kogle ME , Clum A , Lipzen A , Salamov A , Ngan CY , Daum C , Chiniquy J , Barry K , LaButti K , Haridas S , Simmons BA , Magnuson JK , Mortensen UH , Larsen TO , Grigoriev IV , Baker SE , Andersen MR
Ref : Proc Natl Acad Sci U S A , 115 :E753 , 2018
Abstract : The fungal genus of Aspergillus is highly interesting, containing everything from industrial cell factories, model organisms, and human pathogens. In particular, this group has a prolific production of bioactive secondary metabolites (SMs). In this work, four diverse Aspergillus species (A. campestris, A. novofumigatus, A. ochraceoroseus, and A. steynii) have been whole-genome PacBio sequenced to provide genetic references in three Aspergillus sections. A. taichungensis and A. candidus also were sequenced for SM elucidation. Thirteen Aspergillus genomes were analyzed with comparative genomics to determine phylogeny and genetic diversity, showing that each presented genome contains 15-27% genes not found in other sequenced Aspergilli. In particular, A. novofumigatus was compared with the pathogenic species A. fumigatus This suggests that A. novofumigatus can produce most of the same allergens, virulence, and pathogenicity factors as A. fumigatus, suggesting that A. novofumigatus could be as pathogenic as A. fumigatus Furthermore, SMs were linked to gene clusters based on biological and chemical knowledge and analysis, genome sequences, and predictive algorithms. We thus identify putative SM clusters for aflatoxin, chlorflavonin, and ochrindol in A. ochraceoroseus, A. campestris, and A. steynii, respectively, and novofumigatonin, ent-cycloechinulin, and epi-aszonalenins in A. novofumigatus Our study delivers six fungal genomes, showing the large diversity found in the Aspergillus genus; highlights the potential for discovery of beneficial or harmful SMs; and supports reports of A. novofumigatus pathogenicity. It also shows how biological, biochemical, and genomic information can be combined to identify genes involved in the biosynthesis of specific SMs.
ESTHER : Kjaerbolling_2018_Proc.Natl.Acad.Sci.U.S.A_115_E753
PubMedSearch : Kjaerbolling_2018_Proc.Natl.Acad.Sci.U.S.A_115_E753
PubMedID: 29317534
Gene_locus related to this paper: 9euro-a0a0f8xhh7 , 9euro-a0a2t5ll04 , aspn1-nvfd

Title : Genomics and Development of Lentinus tigrinus: A White-Rot Wood-Decaying Mushroom with Dimorphic Fruiting Bodies - Wu_2018_Genome.Biol.Evol_10_3250
Author(s) : Wu B , Xu Z , Knudson A , Carlson A , Chen N , Kovaka S , LaButti K , Lipzen A , Pennachio C , Riley R , Schakwitz W , Umezawa K , Ohm RA , Grigoriev IV , Nagy LG , Gibbons J , Hibbett D
Ref : Genome Biol Evol , 10 :3250 , 2018
Abstract : Lentinus tigrinus is a species of wood-decaying fungi (Polyporales) that has an agaricoid form (a gilled mushroom) and a secotioid form (puffball-like, with enclosed spore-bearing structures). Previous studies suggested that the secotioid form is conferred by a recessive allele of a single locus. We sequenced the genomes of one agaricoid (Aga) strain and one secotioid (Sec) strain (39.53-39.88 Mb, with 15,581-15,380 genes, respectively). We mated the Sec and Aga monokaryons, genotyped the progeny, and performed bulked segregant analysis (BSA). We also fruited three Sec/Sec and three Aga/Aga dikaryons, and sampled transcriptomes at four developmental stages. Using BSA, we identified 105 top candidate genes with nonsynonymous SNPs that cosegregate with fruiting body phenotype. Transcriptome analyses of Sec/Sec versus Aga/Aga dikaryons identified 907 differentially expressed genes (DEGs) along four developmental stages. On the basis of BSA and DEGs, the top 25 candidate genes related to fruiting body development span 1.5 Mb (4% of the genome), possibly on a single chromosome, although the precise locus that controls the secotioid phenotype is unresolved. The top candidates include genes encoding a cytochrome P450 and an ATP-dependent RNA helicase, which may play a role in development, based on studies in other fungi.
ESTHER : Wu_2018_Genome.Biol.Evol_10_3250
PubMedSearch : Wu_2018_Genome.Biol.Evol_10_3250
PubMedID: 30398645
Gene_locus related to this paper: 9aphy-a0a5c2t2q2

Title : Genome expansion and lineage-specific genetic innovations in the forest pathogenic fungi Armillaria - Sipos_2017_Nat.Ecol.Evol_1_1931
Author(s) : Sipos G , Prasanna AN , Walter MC , O'Connor E , Balint B , Krizsan K , Kiss B , Hess J , Varga T , Slot J , Riley R , Boka B , Rigling D , Barry K , Lee J , Mihaltcheva S , LaButti K , Lipzen A , Waldron R , Moloney NM , Sperisen C , Kredics L , Vagvolgyi C , Patrignani A , Fitzpatrick D , Nagy I , Doyle S , Anderson JB , Grigoriev IV , Guldener U , Munsterkotter M , Nagy LG
Ref : Nat Ecol Evol , 1 :1931 , 2017
Abstract : Armillaria species are both devastating forest pathogens and some of the largest terrestrial organisms on Earth. They forage for hosts and achieve immense colony sizes via rhizomorphs, root-like multicellular structures of clonal dispersal. Here, we sequenced and analysed the genomes of four Armillaria species and performed RNA sequencing and quantitative proteomic analysis on the invasive and reproductive developmental stages of A. ostoyae. Comparison with 22 related fungi revealed a significant genome expansion in Armillaria, affecting several pathogenicity-related genes, lignocellulose-degrading enzymes and lineage-specific genes expressed during rhizomorph development. Rhizomorphs express an evolutionarily young transcriptome that shares features with the transcriptomes of both fruiting bodies and vegetative mycelia. Several genes show concomitant upregulation in rhizomorphs and fruiting bodies and share cis-regulatory signatures in their promoters, providing genetic and regulatory insights into complex multicellularity in fungi. Our results suggest that the evolution of the unique dispersal and pathogenicity mechanisms of Armillaria might have drawn upon ancestral genetic toolkits for wood-decay, morphogenesis and complex multicellularity.
ESTHER : Sipos_2017_Nat.Ecol.Evol_1_1931
PubMedSearch : Sipos_2017_Nat.Ecol.Evol_1_1931
PubMedID: 29085064
Gene_locus related to this paper: armos-armb

Title : Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus - de Vries_2017_Genome.Biol_18_28
Author(s) : de Vries RP , Riley R , Wiebenga A , Aguilar-Osorio G , Amillis S , Uchima CA , Anderluh G , Asadollahi M , Askin M , Barry K , Battaglia E , Bayram O , Benocci T , Braus-Stromeyer SA , Caldana C , Canovas D , Cerqueira GC , Chen F , Chen W , Choi C , Clum A , Dos Santos RA , Damasio AR , Diallinas G , Emri T , Fekete E , Flipphi M , Freyberg S , Gallo A , Gournas C , Habgood R , Hainaut M , Harispe ML , Henrissat B , Hilden KS , Hope R , Hossain A , Karabika E , Karaffa L , Karanyi Z , Krasevec N , Kuo A , Kusch H , LaButti K , Lagendijk EL , Lapidus A , Levasseur A , Lindquist E , Lipzen A , Logrieco AF , Maccabe A , Makela MR , Malavazi I , Melin P , Meyer V , Mielnichuk N , Miskei M , Molnar AP , Mule G , Ngan CY , Orejas M , Orosz E , Ouedraogo JP , Overkamp KM , Park HS , Perrone G , Piumi F , Punt PJ , Ram AF , Ramon A , Rauscher S , Record E , Riano-Pachon DM , Robert V , Rohrig J , Ruller R , Salamov A , Salih NS , Samson RA , Sandor E , Sanguinetti M , Schutze T , Sepcic K , Shelest E , Sherlock G , Sophianopoulou V , Squina FM , Sun H , Susca A , Todd RB , Tsang A , Unkles SE , van de Wiele N , van Rossen-Uffink D , Oliveira JV , Vesth TC , Visser J , Yu JH , Zhou M , Andersen MR , Archer DB , Baker SE , Benoit I , Brakhage AA , Braus GH , Fischer R , Frisvad JC , Goldman GH , Houbraken J , Oakley B , Pocsi I , Scazzocchio C , Seiboth B , vanKuyk PA , Wortman J , Dyer PS , Grigoriev IV
Ref : Genome Biol , 18 :28 , 2017
Abstract : BACKGROUND: The fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to investigate aspects of fungal biology, raising questions about evolution and specialization within this genus. RESULTS: We have generated genome sequences for ten novel, highly diverse Aspergillus species and compared these in detail to sister and more distant genera. Comparative studies of key aspects of fungal biology, including primary and secondary metabolism, stress response, biomass degradation, and signal transduction, revealed both conservation and diversity among the species. Observed genomic differences were validated with experimental studies. This revealed several highlights, such as the potential for sex in asexual species, organic acid production genes being a key feature of black aspergilli, alternative approaches for degrading plant biomass, and indications for the genetic basis of stress response. A genome-wide phylogenetic analysis demonstrated in detail the relationship of the newly genome sequenced species with other aspergilli. CONCLUSIONS: Many aspects of biological differences between fungal species cannot be explained by current knowledge obtained from genome sequences. The comparative genomics and experimental study, presented here, allows for the first time a genus-wide view of the biological diversity of the aspergilli and in many, but not all, cases linked genome differences to phenotype. Insights gained could be exploited for biotechnological and medical applications of fungi.
ESTHER : de Vries_2017_Genome.Biol_18_28
PubMedSearch : de Vries_2017_Genome.Biol_18_28
PubMedID: 28196534
Gene_locus related to this paper: asptu-a0a1l9nhd0 , aspve-a0a1l9pxx8 , aspve-a0a1l9q4m3 , aspwe-a0a1l9s133 , 9euro-a0a1l9t3v9 , aspwe-a0a1l9rcx6 , aspna-g3y5a6 , aspgl-a0a1l9v4d3 , 9euro-a0a1l9sa36 , aspsb-a0a319eji6 , aspve-a0a1l9px96 , 9euro-a0a1l9tay1 , aspgl-a0a1l9vbc0 , aspc5-a0a1r3rh65 , 9euro-a0a2v5i956 , aspwe-a0a1l9rpp6 , aspna-g3xpw9 , aspve-a0a1l9plv1 , 9euro-a0a1l9tk47 , aspve-a0a1l9pde9 , aspve-a0a1l9pz72 , aspwe-a0a1l9rde6 , 9euro-a0a1l9tdb5 , aspkw-g7xq95 , aspbc-a0a1l9u6h4 , aspbc-a0a1l9u2l4 , asptc-a0a1l9mx83 , aspgl-a0a1l9ve90 , aspve-a0a1l9pvz9 , 9euro-a0a1l9tdh3 , aspc5-a0a1r3rmn9 , aspwe-a0a1l9rlq2 , asptc-a0a1l9nby7 , aspng-a0a100i8t9 , aspc5-a0a1r3rem6 , aspbc-a0a1l9uy89 , aspa1-anee , aspa1-aneh , aspa1-acrc , aspbc-alba , aspa1-acui

Title : Characterization of four endophytic fungi as potential consolidated bioprocessing hosts for conversion of lignocellulose into advanced biofuels - Wu_2017_Appl.Microbiol.Biotechnol_101_2603
Author(s) : Wu W , Davis RW , Tran-Gyamfi MB , Kuo A , LaButti K , Mihaltcheva S , Hundley H , Chovatia M , Lindquist E , Barry K , Grigoriev IV , Henrissat B , Gladden JM
Ref : Applied Microbiology & Biotechnology , 101 :2603 , 2017
Abstract : Recently, several endophytic fungi have been demonstrated to produce volatile organic compounds (VOCs) with properties similar to fossil fuels, called "mycodiesel," while growing on lignocellulosic plant and agricultural residues. The fact that endophytes are plant symbionts suggests that some may be able to produce lignocellulolytic enzymes, making them capable of both deconstructing lignocellulose and converting it into mycodiesel, two properties that indicate that these strains may be useful consolidated bioprocessing (CBP) hosts for the biofuel production. In this study, four endophytes Hypoxylon sp. CI4A, Hypoxylon sp. EC38, Hypoxylon sp. CO27, and Daldinia eschscholzii EC12 were selected and evaluated for their CBP potential. Analysis of their genomes indicates that these endophytes have a rich reservoir of biomass-deconstructing carbohydrate-active enzymes (CAZys), which includes enzymes active on both polysaccharides and lignin, as well as terpene synthases (TPSs), enzymes that may produce fuel-like molecules, suggesting that they do indeed have CBP potential. GC-MS analyses of their VOCs when grown on four representative lignocellulosic feedstocks revealed that these endophytes produce a wide spectrum of hydrocarbons, the majority of which are monoterpenes and sesquiterpenes, including some known biofuel candidates. Analysis of their cellulase activity when grown under the same conditions revealed that these endophytes actively produce endoglucanases, exoglucanases, and beta-glucosidases. The richness of CAZymes as well as terpene synthases identified in these four endophytic fungi suggests that they are great candidates to pursue for development into platform CBP organisms.
ESTHER : Wu_2017_Appl.Microbiol.Biotechnol_101_2603
PubMedSearch : Wu_2017_Appl.Microbiol.Biotechnol_101_2603
PubMedID: 28078400
Gene_locus related to this paper: 9pezi-a0a1y2u1s8 , 9pezi-a0a1y2x077 , 9pezi-a0a1y2vv92 , 9pezi-a0a1y2txs8 , 9pezi-a0a1y2wzb7 , 9pezi-a0a1y2ufj7 , 9pezi-a0a1y2vvc9 , 9pezi-a0a1y2w3w4

Title : Fungi Contribute Critical but Spatially Varying Roles in Nitrogen and Carbon Cycling in Acid Mine Drainage - Mosier_2016_Front.Microbiol_7_238
Author(s) : Mosier AC , Miller CS , Frischkorn KR , Ohm RA , Li Z , LaButti K , Lapidus A , Lipzen A , Chen C , Johnson J , Lindquist EA , Pan C , Hettich RL , Grigoriev IV , Singer SW , Banfield JF
Ref : Front Microbiol , 7 :238 , 2016
Abstract : The ecosystem roles of fungi have been extensively studied by targeting one organism and/or biological process at a time, but the full metabolic potential of fungi has rarely been captured in an environmental context. We hypothesized that fungal genome sequences could be assembled directly from the environment using metagenomics and that transcriptomics and proteomics could simultaneously reveal metabolic differentiation across habitats. We reconstructed the near-complete 27 Mbp genome of a filamentous fungus, Acidomyces richmondensis, and evaluated transcript and protein expression in floating and streamer biofilms from an acid mine drainage (AMD) system. A. richmondensis transcripts involved in denitrification and in the degradation of complex carbon sources (including cellulose) were up-regulated in floating biofilms, whereas central carbon metabolism and stress-related transcripts were significantly up-regulated in streamer biofilms. These findings suggest that the biofilm niches are distinguished by distinct carbon and nitrogen resource utilization, oxygen availability, and environmental challenges. An isolated A. richmondensis strain from this environment was used to validate the metagenomics-derived genome and confirm nitrous oxide production at pH 1. Overall, our analyses defined mechanisms of fungal adaptation and identified a functional shift related to different roles in carbon and nitrogen turnover for the same species of fungi growing in closely located but distinct biofilm niches.
ESTHER : Mosier_2016_Front.Microbiol_7_238
PubMedSearch : Mosier_2016_Front.Microbiol_7_238
PubMedID: 26973616
Gene_locus related to this paper: 9pezi-a0a150vf31 , 9pezi-a0a150uua7 , 9pezi-a0a150uvr8 , 9pezi-a0a150uzg0 , 9pezi-a0a150v0d4 , 9pezi-a0a150v5h7 , 9pezi-a0a150v662 , 9pezi-a0a150v8z3 , 9pezi-a0a150v9v5 , 9pezi-a0a150vc68 , 9pezi-a0a150vcp1 , 9pezi-a0a150vd70 , 9pezi-a0a150vda0 , 9pezi-a0a150vgv5 , 9pezi-a0a150vil1 , 9pezi-a0a150uu68 , 9pezi-a0a150vas8 , 9pezi-a0a150vji1 , 9pezi-a0a150vii2

Title : Comparative Genomics of Early-Diverging Mushroom-Forming Fungi Provides Insights into the Origins of Lignocellulose Decay Capabilities - Nagy_2016_Mol.Biol.Evol_33_959
Author(s) : Nagy LG , Riley R , Tritt A , Adam C , Daum C , Floudas D , Sun H , Yadav JS , Pangilinan J , Larsson KH , Matsuura K , Barry K , LaButti K , Kuo R , Ohm RA , Bhattacharya SS , Shirouzu T , Yoshinaga Y , Martin FM , Grigoriev IV , Hibbett DS
Ref : Molecular Biology Evolution , 33 :959 , 2016
Abstract : Evolution of lignocellulose decomposition was one of the most ecologically important innovations in fungi. White-rot fungi in the Agaricomycetes (mushrooms and relatives) are the most effective microorganisms in degrading both cellulose and lignin components of woody plant cell walls (PCW). However, the precise evolutionary origins of lignocellulose decomposition are poorly understood, largely because certain early-diverging clades of Agaricomycetes and its sister group, the Dacrymycetes, have yet to be sampled, or have been undersampled, in comparative genomic studies. Here, we present new genome sequences of ten saprotrophic fungi, including members of the Dacrymycetes and early-diverging clades of Agaricomycetes (Cantharellales, Sebacinales, Auriculariales, and Trechisporales), which we use to refine the origins and evolutionary history of the enzymatic toolkit of lignocellulose decomposition. We reconstructed the origin of ligninolytic enzymes, focusing on class II peroxidases (AA2), as well as enzymes that attack crystalline cellulose. Despite previous reports of white rot appearing as early as the Dacrymycetes, our results suggest that white-rot fungi evolved later in the Agaricomycetes, with the first class II peroxidases reconstructed in the ancestor of the Auriculariales and residual Agaricomycetes. The exemplars of the most ancient clades of Agaricomycetes that we sampled all lack class II peroxidases, and are thus concluded to use a combination of plesiomorphic and derived PCW degrading enzymes that predate the evolution of white rot.
ESTHER : Nagy_2016_Mol.Biol.Evol_33_959
PubMedSearch : Nagy_2016_Mol.Biol.Evol_33_959
PubMedID: 26659563
Gene_locus related to this paper: 9homo-a0a164swv2 , 9homo-a0a164tl22 , 9homo-a0a164vkv1 , 9homo-a0a164y4j9 , 9homo-a0a164y4l9 , 9homo-a0a164ytj2 , 9homo-a0a164zeu0 , exigl-a0a165as65 , exigl-a0a165ck85 , 9basi-a0a165cm83 , 9aphy-a0a165dbf1 , 9aphy-a0a165dbf3 , 9basi-a0a165dcb7 , 9aphy-a0a165ddj9 , exigl-a0a165dj22 , exigl-a0a165dmd7 , 9aphy-a0a165egr5 , 9basi-a0a165ekd3 , 9basi-a0a165enc9 , 9basi-a0a165end6 , 9basi-a0a165epz3 , 9basi-a0a165eq46 , 9basi-a0a165eq95 , 9basi-a0a165eqk7 , 9basi-a0a165eup2 , 9aphy-a0a165fb02 , 9aphy-a0a165fmr9 , 9aphy-a0a165g3p1 , 9basi-a0a165g673 , 9basi-a0a165g6g2 , 9aphy-a0a165gec2 , 9basi-a0a165gfp7 , 9aphy-a0a165h505 , 9aphy-a0a165hd72 , 9aphy-a0a165hrk1 , exigl-a0a165hzc8 , 9basi-a0a165i2y7 , 9basi-a0a165iru3 , 9basi-a0a165is19 , 9basi-a0a165is40 , exigl-a0a165j2r4 , exigl-a0a165j754 , 9basi-a0a165jg92 , 9basi-a0a165jgb0 , exigl-a0a165ke04 , exigl-a0a165kpb0 , exigl-a0a165l7g6 , 9aphy-a0a165lux8 , 9aphy-a0a165luy2.1 , 9aphy-a0a165luy2.2 , exigl-a0a165m310 , 9aphy-a0a165mxa5 , 9aphy-a0a165mxc8 , 9aphy-a0a165mxe4 , exigl-a0a165mz73 , 9homo-a0a165nfz4 , 9homo-a0a165ng04 , 9aphy-a0a165nry3 , 9homo-a0a165ntf3 , 9homo-a0a165p2a0 , 9aphy-a0a165ph74 , 9homo-a0a165phz5 , exigl-a0a165pm12 , exigl-a0a165pu90 , exigl-a0a165puh2 , 9aphy-a0a165q9t4 , 9homo-a0a165qeb7 , 9homo-a0a165qeh8 , 9aphy-a0a165qqm2 , 9aphy-a0a165quj0 , 9aphy-a0a165qul3 , exigl-a0a165qxy0 , 9aphy-a0a165r6t2 , 9aphy-a0a165r8g4 , 9aphy-a0a165tfc7 , 9homo-a0a165tgm2 , 9homo-a0a165tzv4 , 9homo-a0a165tzw4 , 9homo-a0a165uez3 , 9homo-a0a165ugh4 , 9homo-a0a165ugp4 , 9homo-a0a165ugq6 , 9homo-a0a165uh51 , 9aphy-a0a165umj9 , 9homo-a0a165us14 , 9homo-a0a165xi11 , 9homo-a0a165xmw5 , 9homo-a0a165xmx9 , 9homo-a0a165xsk7 , 9homo-a0a165y3k8 , 9homo-a0a165yhg3 , 9homo-a0a165ymb3 , 9homo-a0a165yt77 , 9homo-a0a165ytu4 , 9homo-a0a165zr30 , 9homo-a0a166a1g9 , 9homo-a0a166a1h1 , exigl-a0a166abe4 , 9homo-a0a166akq6 , exigl-a0a166al33 , 9homo-a0a166arm1 , 9homo-a0a166as45 , 9homo-a0a166as65 , 9homo-a0a166as77 , exigl-a0a166auh4 , 9homo-a0a166azk8 , exigl-a0a166azz6 , 9homo-a0a166bss2 , 9homo-a0a166bsu9 , 9homo-a0a166bui6 , 9homo-a0a166crl5 , 9homo-a0a166d8q4 , 9homo-a0a166dss7 , 9homo-a0a166dtg8 , 9homo-a0a166du49 , 9homo-a0a166du64 , 9homo-a0a166e1z2 , 9homo-a0a166eec5 , 9homo-a0a166eux5 , 9homo-a0a166evw5 , 9homo-a0a166ey75 , 9homo-a0a166eyq0 , 9homo-a0a166ez78 , 9homo-a0a166eze7 , 9homo-a0a166fh25 , 9homo-a0a166g4k8 , 9homo-a0a166gct1 , 9homo-a0a166gf56 , 9homo-a0a166gsr8 , 9homo-a0a166j6i1 , 9homo-a0a166jiu0 , 9homo-a0a166jr36 , 9homo-a0a166kia8 , 9homo-a0a166ks21 , 9homo-a0a166kvn8 , 9homo-a0a166kxf0 , 9homo-a0a166kxg2 , 9homo-a0a166lcs7 , 9homo-a0a166lw48 , 9homo-a0a166lyz4 , 9homo-a0a166puu7.1 , 9homo-a0a166puu7.2 , 9homo-a0a166puz0 , 9homo-a0a166pv75 , 9homo-a0a166pva0 , 9homo-a0a166pvf7 , 9homo-a0a166px11 , 9homo-a0a166q635 , 9basi-a0a167gl88 , 9basi-a0a167gl97 , 9basi-a0a167gla5 , 9basi-a0a167hca0 , 9basi-a0a167hrt3 , 9basi-a0a167hru7 , 9basi-a0a167k219 , 9basi-a0a167k232 , 9basi-a0a167k265 , 9basi-a0a167l6m6 , 9basi-a0a167lrl7 , 9basi-a0a167lt70 , 9basi-a0a167mtk7 , 9basi-a0a167n1z0 , 9basi-a0a167p9x5 , 9basi-a0a167qks1 , 9basi-a0a167qkt4 , 9basi-a0a167qky5 , 9basi-a0a167qln8 , 9basi-a0a167rpp7 , 9homo-a0a167u8e3 , 9homo-a0a167v1m8 , 9homo-a0a166hqx0 , 9homo-a0a166l842 , exigl-a0a165n4f2 , exigl-a0a165q512 , 9agam-a0a165nq75 , 9agam-a0a166cv75 , 9agam-a0a165mvt4 , 9agam-a0a164t8q2 , 9agam-a0a166flp0

Title : The genome of Xylona heveae provides a window into fungal endophytism - Gazis_2016_Fungal.Biol_120_26
Author(s) : Gazis R , Kuo A , Riley R , LaButti K , Lipzen A , Lin J , Amirebrahimi M , Hesse CN , Spatafora JW , Henrissat B , Hainaut M , Grigoriev IV , Hibbett DS
Ref : Fungal Biol , 120 :26 , 2016
Abstract : Xylona heveae has only been isolated as an endophyte of rubber trees. In an effort to understand the genetic basis of endophytism, we compared the genome contents of X. heveae and 36 other Ascomycota with diverse lifestyles and nutritional modes. We focused on genes that are known to be important in the host-fungus interaction interface and that presumably have a role in determining the lifestyle of a fungus. We used phylogenomic data to infer the higher-level phylogenetic position of the Xylonomycetes, and mined ITS sequences to explore its taxonomic and ecological diversity. The X. heveae genome contains a low number of enzymes needed for plant cell wall degradation, suggesting that Xylona is a highly adapted specialist and likely dependent on its host for survival. The reduced repertoire of carbohydrate active enzymes could reflect an adaptation to intercellulary growth and to the avoidance of the host's immune system, suggesting that Xylona has a strictly endophytic lifestyle. Phylogenomic data resolved the position of Xylonomycetes as sister to Lecanoromycetes and Eurotiomycetes and placed the beetle-endosymbiont Symbiotaphrina as a member of this class. ITS data revealed that Trinosporium is also part of the Xylonomycetes, extending the taxonomic and ecological diversity of this group.
ESTHER : Gazis_2016_Fungal.Biol_120_26
PubMedSearch : Gazis_2016_Fungal.Biol_120_26
PubMedID: 26693682
Gene_locus related to this paper: 9pezi-a0a165f9w1 , 9pezi-a0a165fsb2 , 9pezi-a0a165gpf2 , 9pezi-a0a164zp96 , xylht-a0a165aju9 , xylht-a0a165jye6 , xylht-a0a165jir4

Title : Evolution of novel wood decay mechanisms in Agaricales revealed by the genome sequences of Fistulina hepatica and Cylindrobasidium torrendii - Floudas_2015_Fungal.Genet.Biol_76_78
Author(s) : Floudas D , Held BW , Riley R , Nagy LG , Koehler G , Ransdell AS , Younus H , Chow J , Chiniquy J , Lipzen A , Tritt A , Sun H , Haridas S , LaButti K , Ohm RA , Kues U , Blanchette RA , Grigoriev IV , Minto RE , Hibbett DS
Ref : Fungal Genet Biol , 76 :78 , 2015
Abstract : Wood decay mechanisms in Agaricomycotina have been traditionally separated in two categories termed white and brown rot. Recently the accuracy of such a dichotomy has been questioned. Here, we present the genome sequences of the white-rot fungus Cylindrobasidium torrendii and the brown-rot fungus Fistulina hepatica both members of Agaricales, combining comparative genomics and wood decay experiments. C. torrendii is closely related to the white-rot root pathogen Armillaria mellea, while F. hepatica is related to Schizophyllum commune, which has been reported to cause white rot. Our results suggest that C. torrendii and S. commune are intermediate between white-rot and brown-rot fungi, but at the same time they show characteristics of decay that resembles soft rot. Both species cause weak wood decay and degrade all wood components but leave the middle lamella intact. Their gene content related to lignin degradation is reduced, similar to brown-rot fungi, but both have maintained a rich array of genes related to carbohydrate degradation, similar to white-rot fungi. These characteristics appear to have evolved from white-rot ancestors with stronger ligninolytic ability. F. hepatica shows characteristics of brown rot both in terms of wood decay genes found in its genome and the decay that it causes. However, genes related to cellulose degradation are still present, which is a plesiomorphic characteristic shared with its white-rot ancestors. Four wood degradation-related genes, homologs of which are frequently lost in brown-rot fungi, show signs of pseudogenization in the genome of F. hepatica. These results suggest that transition toward a brown-rot lifestyle could be an ongoing process in F. hepatica. Our results reinforce the idea that wood decay mechanisms are more diverse than initially thought and that the dichotomous separation of wood decay mechanisms in Agaricomycotina into white rot and brown rot should be revisited.
ESTHER : Floudas_2015_Fungal.Genet.Biol_76_78
PubMedSearch : Floudas_2015_Fungal.Genet.Biol_76_78
PubMedID: 25683379
Gene_locus related to this paper: 9agar-a0a0d6zyq5 , 9agar-a0a0d7a2p9 , 9agar-a0a0d7a2v2 , 9agar-a0a0d7abt2 , 9agar-a0a0d7acd3 , 9agar-a0a0d7acx0 , 9agar-a0a0d7acx9 , 9agar-a0a0d7adg2 , 9agar-a0a0d7a6d0 , 9agar-a0a0d7aen7 , 9agar-a0a0d7aez7 , 9agar-a0a0d7ahq5 , 9agar-a0a0d7akr6 , 9agar-a0a0d7al29 , 9agar-a0a0d7an16 , 9agar-a0a0d7ann7 , 9agar-a0a0d7anv1 , 9homo-a0a0d7atv2 , 9homo-a0a0d7ay28 , 9homo-a0a0d7ayz7 , 9homo-a0a0d7b1w8 , 9homo-a0a0d7b2p0 , 9homo-a0a0d7b4n4 , 9homo-a0a0d7b624 , 9homo-a0a0d7b7r3 , 9homo-a0a0d7b7w3 , 9homo-a0a0d7bac5 , 9homo-a0a0d7bav7 , 9homo-a0a0d7bbx7 , 9homo-a0a0d7bdn7 , 9homo-a0a0d7bgj9 , 9homo-a0a0d7biw2 , 9homo-a0a0d7bqi1 , 9homo-a0a0d7bv80 , 9agar-a0a0d7b6f6 , 9agar-a0a0d7b976 , 9agar-a0a0d7aeu9 , 9agar-a0a0d7ag53 , 9agar-a0a0d7b8a5

Title : Comparative genomics analysis of Trichoderma reesei strains - Koike_2013_Ind.Biotechnol_9_352
Author(s) : Koike H , Aerts A , LaButti K , Grigoriev IV , Baker SE
Ref : Ind Biotech , 9 :352 , 2013
Abstract : Trichoderma reesei is a key fungus for industrial production of lignocellulolytic enzymes. The genome sequences of the T. reesei hyper-cellulolytic strain RUT-C30 and its parental strain QM6a were compared at the nucleotide level. Approximately 97% of the 87 genomic-sequence scaffolds of T. reesei QM6a (33Mb) were found to have the corresponding nucleotide in the 182 genome-sequence scaffolds of RUT-C30 (32Mb). There are 455 loci within the QM6 sequence not detected in the RUT-C30 sequence. Regions at the termini of QM6a scaffolds as well as 14 small scaffolds do not have corresponding regions in RUT-C30 genomic scaffolds. Seventy-eight protein-encoding genes are included within these regions. Mutated nucleotide(s) in 2,371 positions, including short insertion/deletions (indels), were detected in the aligned regions. The predicted protein-coding regions of 97 gene models contain mutations, 34 of which were not previously described. Twenty-seven out of 34 newly discovered genes were found to have mutations in the peptide amino acid sequence. This is in addition to 63 genes described in a previous study based on low coverage sequencing of RUT-C30. These newly identified proteins are involved in signal transduction, transcription, RNA processing and modification, and post-translational modification according to their annotations. Similar distributions of eukaryotic orthologous group (KOG) categories between the mutated and all other proteins suggest random mutation. The roles of the mutated genes and potential regulatory regions in the observed phenotype of RUT-C30 remain to be explored in a targeted fashion.
ESTHER : Koike_2013_Ind.Biotechnol_9_352
PubMedSearch : Koike_2013_Ind.Biotechnol_9_352
Gene_locus related to this paper: hypjr-a0a024s1s9

Title : Comparative genome structure, secondary metabolite, and effector coding capacity across Cochliobolus pathogens - Condon_2013_PLoS.Genet_9_e1003233
Author(s) : Condon BJ , Leng Y , Wu D , Bushley KE , Ohm RA , Otillar R , Martin J , Schackwitz W , Grimwood J , MohdZainudin N , Xue C , Wang R , Manning VA , Dhillon B , Tu ZJ , Steffenson BJ , Salamov A , Sun H , Lowry S , LaButti K , Han J , Copeland A , Lindquist E , Barry K , Schmutz J , Baker SE , Ciuffetti LM , Grigoriev IV , Zhong S , Turgeon BG
Ref : PLoS Genet , 9 :e1003233 , 2013
Abstract : The genomes of five Cochliobolus heterostrophus strains, two Cochliobolus sativus strains, three additional Cochliobolus species (Cochliobolus victoriae, Cochliobolus carbonum, Cochliobolus miyabeanus), and closely related Setosphaeria turcica were sequenced at the Joint Genome Institute (JGI). The datasets were used to identify SNPs between strains and species, unique genomic regions, core secondary metabolism genes, and small secreted protein (SSP) candidate effector encoding genes with a view towards pinpointing structural elements and gene content associated with specificity of these closely related fungi to different cereal hosts. Whole-genome alignment shows that three to five percent of each genome differs between strains of the same species, while a quarter of each genome differs between species. On average, SNP counts among field isolates of the same C. heterostrophus species are more than 25x higher than those between inbred lines and 50x lower than SNPs between Cochliobolus species. The suites of nonribosomal peptide synthetase (NRPS), polyketide synthase (PKS), and SSP-encoding genes are astoundingly diverse among species but remarkably conserved among isolates of the same species, whether inbred or field strains, except for defining examples that map to unique genomic regions. Functional analysis of several strain-unique PKSs and NRPSs reveal a strong correlation with a role in virulence.
ESTHER : Condon_2013_PLoS.Genet_9_e1003233
PubMedSearch : Condon_2013_PLoS.Genet_9_e1003233
PubMedID: 23357949
Gene_locus related to this paper: cocsn-m2rnc6 , coch5-m2tnl8 , coch4-n4xap8 , sett2-r0j560 , cocsn-m2thl9 , coch5-m2v1s2 , coch4-n4xzy1 , cocsn-m2sqr3 , cocsn-m2rnk8 , coch4-n4xdv7 , coch5-m2uds0 , coch5-m2um94 , sett2-r0i8c5 , coch4-n4wlc8 , coch4-n4x9p3 , cocsn-m2rh47 , cocsn-m2qz08 , sett2-r0jqq6 , sett2-r0imb6 , coch4-n4x7u3 , cocsn-m2rv02 , cocsn-m2sy95 , coch5-m2ubd5 , cocsn-m2t3d2 , sett2-r0kl84 , sett2-r0jts7 , coch4-n4x2h3 , sett2-r0jxt9 , coch4-n4x7r9 , cocsn-m2sh75 , cocsn-m2t5z2 , coch5-m2ucf6 , sett2-r0k664 , cocsn-m2t3q1 , sett2-r0k4b4 , cocsn-m2t4i1 , coch5-m2th93 , cocsn-m2svm8 , cocsn-m2s6q4 , cocsn-m2s5h5 , coch4-n4xf94 , sett2-r0kdl8 , cocsn-m2qvi9 , sett2-r0kfg6 , cocsn-m2szq4 , sett2-r0j437 , coch4-n4x7j4 , coch5-m2twk3 , coch5-m2usf2 , sett2-r0kjt7 , sett2-r0k7y2 , cocsn-m2th03 , sett2-r0iy92 , sett2-r0kbr9 , sett2-r0k997 , coch5-m2sik6 , sett2-r0jzj5 , cocsn-m2r0j6 , coch4-n4x6a4 , cocsn-m2s7a5 , cocsn-m2sv79 , sett2-r0knx4 , sett2-r0ksh8 , sett2-r0ip86 , cocmi-w6yyy3 , cocsn-m2sqe4 , coch4-n4xzc8 , cocvi-w7eyp1 , cocmi-w6zf65 , cocvi-w7er28 , cocca-w6yw25 , cocvi-w7e2g6 , cocmi-w6z7k5 , cocca-w6ys73 , cocca-w6ydq2 , cocca-w6y7i5 , cocmi-w6yyr0 , cocca-w6yh47 , cocmi-w6zju4 , cocca-w6ynq5 , cocmi-w6zm44 , cocca-w6xx85 , cocmi-w6z011 , cocca-w6yre4 , cocmi-w6z9l3 , cocca-w6yfp7 , cocmi-w6zlc2 , cocca-w6yar2 , cocmi-w6yjr7 , cocca-w6yhs1 , cocca-w6xux8 , cocmi-w6z9s8 , cocca-w6yq27 , cocmi-w6zqk9 , cocca-w6xq19 , cocca-w6y1r6 , cocca-w6ygj2 , cocmi-w6zgn4 , cocca-w6ybh2 , cocmi-w6z710 , cocca-w6yk86 , cocmi-w6zjz2 , cocmi-w6z7f2 , cocca-w6xn57 , cocca-w6ybq4 , cocmi-w6yxn5 , cocmi-w6zf08 , cocsn-m2rtg8 , cocmi-w6zuj7 , cocca-w6xtb2 , cocca-w6yk97 , coch5-m2t2x3 , cocmi-w6z646 , cocsn-m2sze4 , sett2-r0kjg6 , cocmi-w6yrn5 , sett2-r0k5q0 , cocvi-w7ezb7 , sett2-r0jtm1 , cocmi-w6ywa1 , cocsn-m2t3e8 , coch5-m2ulw5 , coch5-m2urw9 , sett2-r0knn5 , cocmi-w6ysb2 , cocvi-w7eag7 , cocca-w6y1v2 , sett2-r0i9k2 , coch5-m2uul8 , cocsn-m2sl21

Title : The genome of the xerotolerant mold Wallemia sebi reveals adaptations to osmotic stress and suggests cryptic sexual reproduction - Padamsee_2012_Fungal.Genet.Biol_49_217
Author(s) : Padamsee M , Kumar TK , Riley R , Binder M , Boyd A , Calvo AM , Furukawa K , Hesse C , Hohmann S , James TY , LaButti K , Lapidus A , Lindquist E , Lucas S , Miller K , Shantappa S , Grigoriev IV , Hibbett DS , McLaughlin DJ , Spatafora JW , Aime MC
Ref : Fungal Genet Biol , 49 :217 , 2012
Abstract : Wallemia (Wallemiales, Wallemiomycetes) is a genus of xerophilic Fungi of uncertain phylogenetic position within Basidiomycota. Most commonly found as food contaminants, species of Wallemia have also been isolated from hypersaline environments. The ability to tolerate environments with reduced water activity is rare in Basidiomycota. We sequenced the genome of W. sebi in order to understand its adaptations for surviving in osmotically challenging environments, and we performed phylogenomic and ultrastructural analyses to address its systematic placement and reproductive biology. W. sebi has a compact genome (9.8 Mb), with few repeats and the largest fraction of genes with functional domains compared with other Basidiomycota. We applied several approaches to searching for osmotic stress-related proteins. In silico analyses identified 93 putative osmotic stress proteins; homology searches showed the HOG (High Osmolarity Glycerol) pathway to be mostly conserved. Despite the seemingly reduced genome, several gene family expansions and a high number of transporters (549) were found that also provide clues to the ability of W. sebi to colonize harsh environments. Phylogenetic analyses of a 71-protein dataset support the position of Wallemia as the earliest diverging lineage of Agaricomycotina, which is confirmed by septal pore ultrastructure that shows the septal pore apparatus as a variant of the Tremella-type. Mating type gene homologs were identified although we found no evidence of meiosis during conidiogenesis, suggesting there may be aspects of the life cycle of W. sebi that remain cryptic.
ESTHER : Padamsee_2012_Fungal.Genet.Biol_49_217
PubMedSearch : Padamsee_2012_Fungal.Genet.Biol_49_217
PubMedID: 22326418
Gene_locus related to this paper: walsc-i4y6w1 , walmc-i4y5m3

Title : Comparative genomics of the white-rot fungi, Phanerochaete carnosa and P. chrysosporium, to elucidate the genetic basis of the distinct wood types they colonize - Suzuki_2012_BMC.Genomics_13_444
Author(s) : Suzuki H , MacDonald J , Syed K , Salamov A , Hori C , Aerts A , Henrissat B , Wiebenga A , vanKuyk PA , Barry K , Lindquist E , LaButti K , Lapidus A , Lucas S , Coutinho P , Gong Y , Samejima M , Mahadevan R , Abou-Zaid M , de Vries RP , Igarashi K , Yadav JS , Grigoriev IV , Master ER
Ref : BMC Genomics , 13 :444 , 2012
Abstract : BACKGROUND: Softwood is the predominant form of land plant biomass in the Northern hemisphere, and is among the most recalcitrant biomass resources to bioprocess technologies. The white rot fungus, Phanerochaete carnosa, has been isolated almost exclusively from softwoods, while most other known white-rot species, including Phanerochaete chrysosporium, were mainly isolated from hardwoods. Accordingly, it is anticipated that P. carnosa encodes a distinct set of enzymes and proteins that promote softwood decomposition. To elucidate the genetic basis of softwood bioconversion by a white-rot fungus, the present study reports the P. carnosa genome sequence and its comparative analysis with the previously reported P. chrysosporium genome.
RESULTS: P. carnosa encodes a complete set of lignocellulose-active enzymes. Comparative genomic analysis revealed that P. carnosa is enriched with genes encoding manganese peroxidase, and that the most divergent glycoside hydrolase families were predicted to encode hemicellulases and glycoprotein degrading enzymes. Most remarkably, P. carnosa possesses one of the largest P450 contingents (266 P450s) among the sequenced and annotated wood-rotting basidiomycetes, nearly double that of P. chrysosporium. Along with metabolic pathway modeling, comparative growth studies on model compounds and chemical analyses of decomposed wood components showed greater tolerance of P. carnosa to various substrates including coniferous heartwood.
CONCLUSIONS: The P. carnosa genome is enriched with genes that encode P450 monooxygenases that can participate in extractives degradation, and manganese peroxidases involved in lignin degradation. The significant expansion of P450s in P. carnosa, along with differences in carbohydrate- and lignin-degrading enzymes, could be correlated to the utilization of heartwood and sapwood preparations from both coniferous and hardwood species.
ESTHER : Suzuki_2012_BMC.Genomics_13_444
PubMedSearch : Suzuki_2012_BMC.Genomics_13_444
PubMedID: 22937793
Gene_locus related to this paper: phacs-k5whx2 , phacs-k5v2s8 , phacs-k5v5r2 , phacs-k5vyk5 , phacs-k5vzf8 , phacs-k5wbu9 , phacs-k5wc10 , phacs-k5wpw0 , phacs-k5wzn6 , phacs-k5x1t8 , phacs-k5x5g6 , phacs-k5x5p4

Title : The Paleozoic origin of enzymatic lignin decomposition reconstructed from 31 fungal genomes - Floudas_2012_Science_336_1715
Author(s) : Floudas D , Binder M , Riley R , Barry K , Blanchette RA , Henrissat B , Martinez AT , Otillar R , Spatafora JW , Yadav JS , Aerts A , Benoit I , Boyd A , Carlson A , Copeland A , Coutinho PM , de Vries RP , Ferreira P , Findley K , Foster B , Gaskell J , Glotzer D , Gorecki P , Heitman J , Hesse C , Hori C , Igarashi K , Jurgens JA , Kallen N , Kersten P , Kohler A , Kues U , Kumar TK , Kuo A , LaButti K , Larrondo LF , Lindquist E , Ling A , Lombard V , Lucas S , Lundell T , Martin R , McLaughlin DJ , Morgenstern I , Morin E , Murat C , Nagy LG , Nolan M , Ohm RA , Patyshakuliyeva A , Rokas A , Ruiz-Duenas FJ , Sabat G , Salamov A , Samejima M , Schmutz J , Slot JC , St John F , Stenlid J , Sun H , Sun S , Syed K , Tsang A , Wiebenga A , Young D , Pisabarro A , Eastwood DC , Martin F , Cullen D , Grigoriev IV , Hibbett DS
Ref : Science , 336 :1715 , 2012
Abstract : Wood is a major pool of organic carbon that is highly resistant to decay, owing largely to the presence of lignin. The only organisms capable of substantial lignin decay are white rot fungi in the Agaricomycetes, which also contains non-lignin-degrading brown rot and ectomycorrhizal species. Comparative analyses of 31 fungal genomes (12 generated for this study) suggest that lignin-degrading peroxidases expanded in the lineage leading to the ancestor of the Agaricomycetes, which is reconstructed as a white rot species, and then contracted in parallel lineages leading to brown rot and mycorrhizal species. Molecular clock analyses suggest that the origin of lignin degradation might have coincided with the sharp decrease in the rate of organic carbon burial around the end of the Carboniferous period.
ESTHER : Floudas_2012_Science_336_1715
PubMedSearch : Floudas_2012_Science_336_1715
PubMedID: 22745431
Gene_locus related to this paper: aurde-j0d098 , aurde-j0dc31 , glota-s7rlc1 , fompi-s8f7s4 , dacsp-m5fpg2 , dicsq-r7sm16 , dacsp-m5g7q5 , dacsp-m5fr12 , glota-s7q5w3 , fompi-s8f826.1 , fompi-s8f826.2 , dicsq-r7sy09 , glota-s7rt87 , dicsq-r7t032 , glota-s7rym7 , fompi-s8fiv2 , dacsp-m5gda3.2 , dicsq-r7swi6 , dacsp-m5frf2 , fompi-s8ebb6 , dicsq-r7sln3 , dicsq-r7sya6 , dacsp-m5g7g1 , dicsq-r7syx7 , dicsq-r7sx57 , dacsp-m5fps7 , glota-s7pwi7 , dicsq-r7swj6 , fompi-s8ejq6 , dicsq-r7spc3 , glota-s7q258 , dacsp-m5ft65 , glota-s7q3m7 , fompi-s8dkc7 , glota-s7q1z1 , fompi-s8eqi2 , glota-s7q1z8 , fompi-s8du50 , dacsp-m5gg33 , dacsp-m5g3a7 , fompi-s8ecd7 , fompi-s8dps1 , dacsp-m5fwr0 , dicsq-r7sub7 , glota-s7q8k9 , fompi-s8ffc3 , dacsp-m5g2f9 , fompi-s8ecc2 , dacsp-m5g868 , fompi-s8f890 , dicsq-r7t1a8 , fompi-s8ebx4 , fompi-s8eb97 , glota-s7q222 , glota-s7puf0 , fompi-s8f6v9 , dacsp-m5g0z2 , dacsp-m5gdh9 , fompi-s8fb37 , dacsp-m5fy91 , glota-s7q5v6 , fompi-s8fl44 , dicsq-r7stv9 , dicsq-r7szk3 , fompi-s8epq9 , glota-s7rh56 , dacsp-m5gbt1 , punst-r7s3x9 , punst-r7s0t5 , glota-s7q312 , glota-s7rhh6 , dicsq-r7t117 , dicsq-r7slz3

Title : Diverse lifestyles and strategies of plant pathogenesis encoded in the genomes of eighteen Dothideomycetes fungi - Ohm_2012_PLoS.Pathog_8_e1003037
Author(s) : Ohm RA , Feau N , Henrissat B , Schoch CL , Horwitz BA , Barry KW , Condon BJ , Copeland AC , Dhillon B , Glaser F , Hesse CN , Kosti I , LaButti K , Lindquist EA , Lucas S , Salamov AA , Bradshaw RE , Ciuffetti L , Hamelin RC , Kema GH , Lawrence C , Scott JA , Spatafora JW , Turgeon BG , de Wit PJ , Zhong S , Goodwin SB , Grigoriev IV
Ref : PLoS Pathog , 8 :e1003037 , 2012
Abstract : The class Dothideomycetes is one of the largest groups of fungi with a high level of ecological diversity including many plant pathogens infecting a broad range of hosts. Here, we compare genome features of 18 members of this class, including 6 necrotrophs, 9 (hemi)biotrophs and 3 saprotrophs, to analyze genome structure, evolution, and the diverse strategies of pathogenesis. The Dothideomycetes most likely evolved from a common ancestor more than 280 million years ago. The 18 genome sequences differ dramatically in size due to variation in repetitive content, but show much less variation in number of (core) genes. Gene order appears to have been rearranged mostly within chromosomal boundaries by multiple inversions, in extant genomes frequently demarcated by adjacent simple repeats. Several Dothideomycetes contain one or more gene-poor, transposable element (TE)-rich putatively dispensable chromosomes of unknown function. The 18 Dothideomycetes offer an extensive catalogue of genes involved in cellulose degradation, proteolysis, secondary metabolism, and cysteine-rich small secreted proteins. Ancestors of the two major orders of plant pathogens in the Dothideomycetes, the Capnodiales and Pleosporales, may have had different modes of pathogenesis, with the former having fewer of these genes than the latter. Many of these genes are enriched in proximity to transposable elements, suggesting faster evolution because of the effects of repeat induced point (RIP) mutations. A syntenic block of genes, including oxidoreductases, is conserved in most Dothideomycetes and upregulated during infection in L. maculans, suggesting a possible function in response to oxidative stress.
ESTHER : Ohm_2012_PLoS.Pathog_8_e1003037
PubMedSearch : Ohm_2012_PLoS.Pathog_8_e1003037
PubMedID: 23236275
Gene_locus related to this paper: mycpj-q30dw8 , sphms-m3db71 , bauco-m2n3p9 , cocsn-m2rnc6 , coch5-m2tnl8 , coch4-n4xap8 , sett2-r0j560 , bauco-m2lw45 , cocsn-m2thl9 , bauco-m2nan7 , sphms-m3asf7 , coch5-m2v1s2 , mycfi-m3am36 , coch4-n4xzy1 , mycfi-m3b3x0 , cocsn-m2sqr3 , cocsn-m2rnk8 , mycp1-n1pnd6 , bauco-m2n7y7 , coch4-n4xdv7 , coch5-m2uds0 , coch5-m2um94 , sett2-r0i8c5 , coch4-n4wlc8 , coch4-n4x9p3 , cocsn-m2rh47 , cocsn-m2qz08 , sett2-r0jqq6 , mycfi-m2yiq2 , sett2-r0imb6 , sphms-m3b727 , coch4-n4x7u3 , cocsn-m2rv02 , cocsn-m2sy95 , coch5-m2ubd5 , mycp1-n1per0 , mycp1-n1pg49 , mycfi-n1q8u1 , mycp1-n1pwj1 , mycp1-n1pcl8 , bauco-m2n330 , cocsn-m2t3d2 , mycfi-m3b223 , sett2-r0kl84 , bauco-m2lu86 , mycfi-m3b1s8 , sett2-r0jts7 , mycfi-m3amn9 , bauco-m2nf03 , mycfi-m3a015 , sphms-n1qgv4 , coch4-n4x2h3 , mycp1-m2y2b1 , sett2-r0jxt9 , mycfi-m2zg05 , sphms-m3cr09 , coch4-n4x7r9 , mycfi-m2yip7 , mycp1-n1pwu7 , cocsn-m2sh75 , cocsn-m2t5z2 , coch5-m2ucf6 , sphms-m3c9s8 , sphms-m3c383 , mycp1-n1ppa8 , sett2-r0k664 , cocsn-m2t3q1 , sett2-r0k4b4 , cocsn-m2t4i1 , bauco-m2lzw1 , coch5-m2th93 , cocsn-m2svm8 , sphms-m3d7h2 , sphms-m3cwc3 , mycfi-m3b329 , bauco-m2n4x9 , cocsn-m2s6q4 , mycfi-m3b7x7 , mycp1-m2yk59 , cocsn-m2s5h5 , bauco-m2nfr9 , bauco-m2myk4 , coch4-n4xf94 , mycfi-m3a252 , sphms-n1qes8 , mycp1-n1pps5 , sett2-r0kdl8 , cocsn-m2qvi9 , sett2-r0kfg6 , bauco-m2n1q0 , cocsn-m2szq4 , sett2-r0j437 , coch4-n4x7j4 , mycfi-m3b4h3 , coch5-m2twk3 , coch5-m2usf2 , sett2-r0kjt7 , mycfi-m2yrk1 , bauco-m2n4g8 , sett2-r0k7y2 , cocsn-m2th03 , sett2-r0iy92 , sett2-r0kbr9 , sett2-r0k997 , coch5-m2sik6 , bauco-m2n0g0 , bauco-m2lkk0 , sett2-r0jzj5 , sphms-m3bs21 , mycfi-m3a3h8 , mycp1-n1pw13 , cocsn-m2r0j6 , mycp1-n1pe19 , coch4-n4x6a4 , mycp1-m2xhl1 , cocsn-m2s7a5 , cocsn-m2sv79 , mycfi-n1qbd7 , mycp1-n1pnh6 , sphms-m3cz62 , sett2-r0knx4 , bauco-m2nlz2 , mycp1-n1psn5 , sett2-r0ksh8 , bauco-m2n3v9 , bauco-m2n9y7 , mycp1-n1puh9 , sett2-r0ip86 , sphms-m3c6j1 , sphms-n1qnq9 , cocsn-m2sqe4 , coch4-n4xzc8 , mycfi-m3ali0 , mycfi-m3a5j4 , mycp1-n1phf7 , bauco-m2myw5 , mycp1-m2y2h4 , mycfi-m3as05 , sphms-m3ccg5 , cocsn-m2rtg8 , sphms-n1qny5 , mycfi-n1q7c3 , mycp1-n1q523 , bauco-m2m190 , psefd-m3awp8 , sphms-n1qfl1 , dotsn-n1q1b1 , sphms-m3dcu2 , bauco-m2m7v7 , psefd-m3bad8 , bauco-m2nft5 , psefd-m3b4x7 , sphms-n1qdh4 , sphms-m3cq38 , bauco-m2mz43 , coch5-m2t2x3 , cocsn-m2sze4 , sphms-n1qfm9 , sett2-r0kjg6 , sett2-r0k5q0 , cocvi-w7ezb7 , sett2-r0jtm1 , cocmi-w6ywa1 , psefd-m3a663 , baupa-m2mxl2 , cocsn-m2t3e8 , coch5-m2ulw5 , coch5-m2urw9 , sett2-r0knn5 , cocca-w6y1v2 , baupa-m2nq79 , sett2-r0i9k2 , coch5-m2uul8 , dotsn-n1q415 , psefd-n1qcy3 , cocsn-m2sl21 , baupa-m2luc8 , dotsn-est1

Title : The plant cell wall-decomposing machinery underlies the functional diversity of forest fungi - Eastwood_2011_Science_333_762
Author(s) : Eastwood DC , Floudas D , Binder M , Majcherczyk A , Schneider P , Aerts A , Asiegbu FO , Baker SE , Barry K , Bendiksby M , Blumentritt M , Coutinho PM , Cullen D , de Vries RP , Gathman A , Goodell B , Henrissat B , Ihrmark K , Kauserud H , Kohler A , LaButti K , Lapidus A , Lavin JL , Lee YH , Lindquist E , Lilly W , Lucas S , Morin E , Murat C , Oguiza JA , Park J , Pisabarro AG , Riley R , Rosling A , Salamov A , Schmidt O , Schmutz J , Skrede I , Stenlid J , Wiebenga A , Xie X , Kues U , Hibbett DS , Hoffmeister D , Hogberg N , Martin F , Grigoriev IV , Watkinson SC
Ref : Science , 333 :762 , 2011
Abstract : Brown rot decay removes cellulose and hemicellulose from wood--residual lignin contributing up to 30% of forest soil carbon--and is derived from an ancestral white rot saprotrophy in which both lignin and cellulose are decomposed. Comparative and functional genomics of the "dry rot" fungus Serpula lacrymans, derived from forest ancestors, demonstrated that the evolution of both ectomycorrhizal biotrophy and brown rot saprotrophy were accompanied by reductions and losses in specific protein families, suggesting adaptation to an intercellular interaction with plant tissue. Transcriptome and proteome analysis also identified differences in wood decomposition in S. lacrymans relative to the brown rot Postia placenta. Furthermore, fungal nutritional mode diversification suggests that the boreal forest biome originated via genetic coevolution of above- and below-ground biota.
ESTHER : Eastwood_2011_Science_333_762
PubMedSearch : Eastwood_2011_Science_333_762
PubMedID: 21764756
Gene_locus related to this paper: serl3-f8prj2 , serl3-f8qcc4 , serl9-f8ngp6 , serl9-f8nhd7 , serl9-f8nhq9 , serl9-f8nq77 , serl9-f8nr67 , serl9-f8nrt5 , serl9-f8nvy7.1 , serl9-f8nvy7.2 , serl9-f8nvy8 , serl9-f8nxt0.1 , serl9-f8nxt0.2 , serl9-f8nzr3 , serl9-f8p0f0 , serl9-f8p6v0 , serl9-f8p015 , serl9-f8p018 , serl9-f8p386 , serl9-f8paz8 , serl9-f8pbv1 , serl9-f8pby1 , serl9-f8pc25 , serl9-f8pc39 , serl9-f8nia7 , serl3-f8pju2 , serl9-f8peh1 , serl9-nps3

Title : Complete genome sequence of Planctomyces limnophilus type strain (Mu 290) - Labutti_2010_Stand.Genomic.Sci_3_47
Author(s) : LaButti K , Sikorski J , Schneider S , Nolan M , Lucas S , Glavina Del Rio T , Tice H , Cheng JF , Goodwin L , Pitluck S , Liolios K , Ivanova N , Mavromatis K , Mikhailova N , Pati A , Chen A , Palaniappan K , Land M , Hauser L , Chang YJ , Jeffries CD , Tindall BJ , Rohde M , Goker M , Woyke T , Bristow J , Eisen JA , Markowitz V , Hugenholtz P , Kyrpides NC , Klenk HP , Lapidus A
Ref : Stand Genomic Sci , 3 :47 , 2010
Abstract : Planctomyces limnophilus Hirsch and Muller 1986 belongs to the order Planctomycetales, which differs from other bacterial taxa by several distinctive features such as internal cell compartmentalization, multiplication by forming buds directly from the spherical, ovoid or pear-shaped mother cell and a cell wall which is stabilized by a proteinaceous layer rather than a peptidoglycan layer. Besides Pirellula staleyi, this is the second completed genome sequence of the family Planctomycetaceae. P. limnophilus is of interest because it differs from Pirellula by the presence of a stalk and its structure of fibril bundles, its cell shape and size, the formation of multicellular rosettes, low salt tolerance and red pigmented colonies. The 5,460,085 bp long genome with its 4,304 protein-coding and 66 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
ESTHER : Labutti_2010_Stand.Genomic.Sci_3_47
PubMedSearch : Labutti_2010_Stand.Genomic.Sci_3_47
PubMedID: 21304691
Gene_locus related to this paper: plal2-d5spy8 , plal2-d5ssg7 , plal2-d5ssq1 , plal2-d5stl8 , plal2-d5su74 , plal2-d5swy9 , plal2-d5sxa1 , plal2-d5sxi9 , plal2-d5swp5

Title : Complete genome sequence of Sphaerobacter thermophilus type strain (S 6022) - Pati_2010_Stand.Genomic.Sci_2_49
Author(s) : Pati A , LaButti K , Pukall R , Nolan M , Glavina Del Rio T , Tice H , Cheng JF , Lucas S , Chen F , Copeland A , Ivanova N , Mavromatis K , Mikhailova N , Pitluck S , Bruce D , Goodwin L , Land M , Hauser L , Chang YJ , Jeffries CD , Chen A , Palaniappan K , Chain P , Brettin T , Sikorski J , Rohde M , Goker M , Bristow J , Eisen JA , Markowitz V , Hugenholtz P , Kyrpides NC , Klenk HP , Lapidus A
Ref : Stand Genomic Sci , 2 :49 , 2010
Abstract : Sphaerobacter thermophilus Demharter et al. 1989 is the sole and type species of the genus Sphaerobacter, which is the type genus of the family Sphaerobacteraceae, the order Sphaerobacterales and the subclass Sphaerobacteridae. Phylogenetically, it belongs to the genomically little studied class of the Thermomicrobia in the bacterial phylum Chloroflexi. Here, the genome of strain S 6022(T) is described which is an obligate aerobe that was originally isolated from an aerated laboratory-scale fermentor that was pulse fed with municipal sewage sludge. We describe the features of this organism, together with the complete genome and annotation. This is the first complete genome sequence of the thermomicrobial subclass Sphaerobacteridae, and the second sequence from the chloroflexal class Thermomicrobia. The 3,993,764 bp genome with its 3,525 protein-coding and 57 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
ESTHER : Pati_2010_Stand.Genomic.Sci_2_49
PubMedSearch : Pati_2010_Stand.Genomic.Sci_2_49
PubMedID: 21304677

Title : Permanent draft genome sequence of Dethiosulfovibrio peptidovorans type strain (SEBR 4207) - Labutti_2010_Stand.Genomic.Sci_3_85
Author(s) : LaButti K , Mayilraj S , Clum A , Lucas S , Glavina Del Rio T , Nolan M , Tice H , Cheng JF , Pitluck S , Liolios K , Ivanova N , Mavromatis K , Mikhailova N , Pati A , Goodwin L , Chen A , Palaniappan K , Land M , Hauser L , Chang YJ , Jeffries CD , Rohde M , Spring S , Goker M , Woyke T , Bristow J , Eisen JA , Markowitz V , Hugenholtz P , Kyrpides NC , Klenk HP , Lapidus A
Ref : Stand Genomic Sci , 3 :85 , 2010
Abstract : Dethiosulfovibrio peptidovorans Magot et al. 1997 is the type species of the genus Dethiosulfovibrio of the family Synergistaceae in the recently created phylum Synergistetes. The strictly anaerobic, vibriod, thiosulfate-reducing bacterium utilizes peptides and amino acids, but neither sugars nor fatty acids. It was isolated from an offshore oil well where it was been reported to be involved in pitting corrosion of mild steel. Initially, this bacterium was described as a distant relative of the genus Thermoanaerobacter, but was not assigned to a genus, it was subsequently placed into the novel phylum Synergistetes. A large number of repeats in the genome sequence prevented an economically justifiable closure of the last gaps. This is only the third published genome from a member of the phylum Synergistetes. The 2,576,359 bp long genome consists of three contigs with 2,458 protein-coding and 59 RNA genes and is part of the Genomic Encyclopedia of Bacteria and Archaea project.
ESTHER : Labutti_2010_Stand.Genomic.Sci_3_85
PubMedSearch : Labutti_2010_Stand.Genomic.Sci_3_85
PubMedID: 21304695

Title : Complete genome sequence of Anaerococcus prevotii type strain (PC1) - Labutti_2009_Stand.Genomic.Sci_1_159
Author(s) : LaButti K , Pukall R , Steenblock K , Glavina Del Rio T , Tice H , Copeland A , Cheng JF , Lucas S , Chen F , Nolan M , Bruce D , Goodwin L , Pitluck S , Ivanova N , Mavromatis K , Ovchinnikova G , Pati A , Chen A , Palaniappan K , Land M , Hauser L , Chang YJ , Jeffries CD , Chain P , Saunders E , Brettin T , Detter JC , Han C , Goker M , Bristow J , Eisen JA , Markowitz V , Hugenholtz P , Kyrpides NC , Klenk HP , Lapidus A
Ref : Stand Genomic Sci , 1 :159 , 2009
Abstract : Anaerococcus prevotii (Foubert and Douglas 1948) Ezaki et al. 2001 is the type species of the genus, and is of phylogenetic interest because of its arguable assignment to the provisionally arranged family 'Peptostreptococcaceae'. A. prevotii is an obligate anaerobic coccus, usually arranged in clumps or tetrads. The strain, whose genome is described here, was originally isolated from human plasma; other strains of the species were also isolated from clinical specimen. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first completed genome sequence of a member of the genus. Next to Finegoldia magna, A. prevotii is only the second species from the family 'Peptostreptococcaceae' for which a complete genome sequence is described. The 1,998,633 bp long genome (chromosome and one plasmid) with its 1852 protein-coding and 61 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
ESTHER : Labutti_2009_Stand.Genomic.Sci_1_159
PubMedSearch : Labutti_2009_Stand.Genomic.Sci_1_159
PubMedID: 21304652
Gene_locus related to this paper: anapd-c7ri43

Title : Genome sequence of Aedes aegypti, a major arbovirus vector - Nene_2007_Science_316_1718
Author(s) : Nene V , Wortman JR , Lawson D , Haas B , Kodira C , Tu ZJ , Loftus B , Xi Z , Megy K , Grabherr M , Ren Q , Zdobnov EM , Lobo NF , Campbell KS , Brown SE , Bonaldo MF , Zhu J , Sinkins SP , Hogenkamp DG , Amedeo P , Arensburger P , Atkinson PW , Bidwell S , Biedler J , Birney E , Bruggner RV , Costas J , Coy MR , Crabtree J , Crawford M , Debruyn B , Decaprio D , Eiglmeier K , Eisenstadt E , El-Dorry H , Gelbart WM , Gomes SL , Hammond M , Hannick LI , Hogan JR , Holmes MH , Jaffe D , Johnston JS , Kennedy RC , Koo H , Kravitz S , Kriventseva EV , Kulp D , LaButti K , Lee E , Li S , Lovin DD , Mao C , Mauceli E , Menck CF , Miller JR , Montgomery P , Mori A , Nascimento AL , Naveira HF , Nusbaum C , O'Leary S , Orvis J , Pertea M , Quesneville H , Reidenbach KR , Rogers YH , Roth CW , Schneider JR , Schatz M , Shumway M , Stanke M , Stinson EO , Tubio JM , Vanzee JP , Verjovski-Almeida S , Werner D , White O , Wyder S , Zeng Q , Zhao Q , Zhao Y , Hill CA , Raikhel AS , Soares MB , Knudson DL , Lee NH , Galagan J , Salzberg SL , Paulsen IT , Dimopoulos G , Collins FH , Birren B , Fraser-Liggett CM , Severson DW
Ref : Science , 316 :1718 , 2007
Abstract : We present a draft sequence of the genome of Aedes aegypti, the primary vector for yellow fever and dengue fever, which at approximately 1376 million base pairs is about 5 times the size of the genome of the malaria vector Anopheles gambiae. Nearly 50% of the Ae. aegypti genome consists of transposable elements. These contribute to a factor of approximately 4 to 6 increase in average gene length and in sizes of intergenic regions relative to An. gambiae and Drosophila melanogaster. Nonetheless, chromosomal synteny is generally maintained among all three insects, although conservation of orthologous gene order is higher (by a factor of approximately 2) between the mosquito species than between either of them and the fruit fly. An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species.
ESTHER : Nene_2007_Science_316_1718
PubMedSearch : Nene_2007_Science_316_1718
PubMedID: 17510324
Gene_locus related to this paper: aedae-ACHE , aedae-ACHE1 , aedae-glita , aedae-q0iea6 , aedae-q0iev6 , aedae-q0ifn6 , aedae-q0ifn8 , aedae-q0ifn9 , aedae-q0ifp0 , aedae-q0ig41 , aedae-q1dgl0 , aedae-q1dh03 , aedae-q1dh19 , aedae-q1hqe6 , aedae-Q8ITU8 , aedae-Q8MMJ6 , aedae-Q8T9V6 , aedae-q16e91 , aedae-q16f04 , aedae-q16f25 , aedae-q16f26 , aedae-q16f28 , aedae-q16f29 , aedae-q16f30 , aedae-q16gq5 , aedae-q16iq5 , aedae-q16je0 , aedae-q16je1 , aedae-q16je2 , aedae-q16ks8 , aedae-q16lf2 , aedae-q16lv6 , aedae-q16m61 , aedae-q16mc1 , aedae-q16mc6 , aedae-q16mc7 , aedae-q16md1 , aedae-q16ms7 , aedae-q16nk5 , aedae-q16rl5 , aedae-q16rz9 , aedae-q16si8 , aedae-q16t49 , aedae-q16wf1 , aedae-q16x18 , aedae-q16xp8 , aedae-q16xu6 , aedae-q16xw5 , aedae-q16xw6 , aedae-q16y04 , aedae-q16y05 , aedae-q16y06 , aedae-q16y07 , aedae-q16y39 , aedae-q16y40 , aedae-q16yg4 , aedae-q16z03 , aedae-q17aa7 , aedae-q17av1 , aedae-q17av2 , aedae-q17av3 , aedae-q17av4 , aedae-q17b28 , aedae-q17b29 , aedae-q17b30 , aedae-q17b31 , aedae-q17b32 , aedae-q17bm3 , aedae-q17bm4 , aedae-q17bv7 , aedae-q17c44 , aedae-q17cz1 , aedae-q17d32 , aedae-q17g39 , aedae-q17g40 , aedae-q17g41 , aedae-q17g42 , aedae-q17g43 , aedae-q17g44 , aedae-q17gb8 , aedae-q17gr3 , aedae-q17if7 , aedae-q17if9 , aedae-q17ig1 , aedae-q17ig2 , aedae-q17is4 , aedae-q17l09 , aedae-q17m26 , aedae-q17mg9 , aedae-q17mv4 , aedae-q17mv5 , aedae-q17mv6 , aedae-q17mv7 , aedae-q17mw8 , aedae-q17mw9 , aedae-q17nw5 , aedae-q17nx5 , aedae-q17pa4 , aedae-q17q69 , aedae-q170k7 , aedae-q171y4 , aedae-q172e0 , aedae-q176i8 , aedae-q176j0 , aedae-q177k1 , aedae-q177k2 , aedae-q177l9 , aedae-j9hic3 , aedae-q179r9 , aedae-u483 , aedae-j9hj23 , aedae-q17d68 , aedae-q177c7 , aedae-q0ifp1 , aedae-a0a1s4fx83 , aedae-a0a1s4g2m0 , aedae-q1hr49

Title : Human chromosome 11 DNA sequence and analysis including novel gene identification - Taylor_2006_Nature_440_497
Author(s) : Taylor TD , Noguchi H , Totoki Y , Toyoda A , Kuroki Y , Dewar K , Lloyd C , Itoh T , Takeda T , Kim DW , She X , Barlow KF , Bloom T , Bruford E , Chang JL , Cuomo CA , Eichler E , Fitzgerald MG , Jaffe DB , LaButti K , Nicol R , Park HS , Seaman C , Sougnez C , Yang X , Zimmer AR , Zody MC , Birren BW , Nusbaum C , Fujiyama A , Hattori M , Rogers J , Lander ES , Sakaki Y
Ref : Nature , 440 :497 , 2006
Abstract : Chromosome 11, although average in size, is one of the most gene- and disease-rich chromosomes in the human genome. Initial gene annotation indicates an average gene density of 11.6 genes per megabase, including 1,524 protein-coding genes, some of which were identified using novel methods, and 765 pseudogenes. One-quarter of the protein-coding genes shows overlap with other genes. Of the 856 olfactory receptor genes in the human genome, more than 40% are located in 28 single- and multi-gene clusters along this chromosome. Out of the 171 disorders currently attributed to the chromosome, 86 remain for which the underlying molecular basis is not yet known, including several mendelian traits, cancer and susceptibility loci. The high-quality data presented here--nearly 134.5 million base pairs representing 99.8% coverage of the euchromatic sequence--provide scientists with a solid foundation for understanding the genetic basis of these disorders and other biological phenomena.
ESTHER : Taylor_2006_Nature_440_497
PubMedSearch : Taylor_2006_Nature_440_497
PubMedID: 16554811
Gene_locus related to this paper: human-PRCP

Title : DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage - Zody_2006_Nature_440_1045
Author(s) : Zody MC , Garber M , Adams DJ , Sharpe T , Harrow J , Lupski JR , Nicholson C , Searle SM , Wilming L , Young SK , Abouelleil A , Allen NR , Bi W , Bloom T , Borowsky ML , Bugalter BE , Butler J , Chang JL , Chen CK , Cook A , Corum B , Cuomo CA , de Jong PJ , Decaprio D , Dewar K , FitzGerald M , Gilbert J , Gibson R , Gnerre S , Goldstein S , Grafham DV , Grocock R , Hafez N , Hagopian DS , Hart E , Norman CH , Humphray S , Jaffe DB , Jones M , Kamal M , Khodiyar VK , LaButti K , Laird G , Lehoczky J , Liu X , Lokyitsang T , Loveland J , Lui A , Macdonald P , Major JE , Matthews L , Mauceli E , McCarroll SA , Mihalev AH , Mudge J , Nguyen C , Nicol R , O'Leary SB , Osoegawa K , Schwartz DC , Shaw-Smith C , Stankiewicz P , Steward C , Swarbreck D , Venkataraman V , Whittaker CA , Yang X , Zimmer AR , Bradley A , Hubbard T , Birren BW , Rogers J , Lander ES , Nusbaum C
Ref : Nature , 440 :1045 , 2006
Abstract : Chromosome 17 is unusual among the human chromosomes in many respects. It is the largest human autosome with orthology to only a single mouse chromosome, mapping entirely to the distal half of mouse chromosome 11. Chromosome 17 is rich in protein-coding genes, having the second highest gene density in the genome. It is also enriched in segmental duplications, ranking third in density among the autosomes. Here we report a finished sequence for human chromosome 17, as well as a structural comparison with the finished sequence for mouse chromosome 11, the first finished mouse chromosome. Comparison of the orthologous regions reveals striking differences. In contrast to the typical pattern seen in mammalian evolution, the human sequence has undergone extensive intrachromosomal rearrangement, whereas the mouse sequence has been remarkably stable. Moreover, although the human sequence has a high density of segmental duplication, the mouse sequence has a very low density. Notably, these segmental duplications correspond closely to the sites of structural rearrangement, demonstrating a link between duplication and rearrangement. Examination of the main classes of duplicated segments provides insight into the dynamics underlying expansion of chromosome-specific, low-copy repeats in the human genome.
ESTHER : Zody_2006_Nature_440_1045
PubMedSearch : Zody_2006_Nature_440_1045
PubMedID: 16625196
Gene_locus related to this paper: human-NLGN2 , human-NOTUM