Bacterial_lip_FamI.1
Relationship
Comment
Lactonizing lipase. This family corresponds to family I.1 of the classification of Arpigny and Jaeger (1999) The pseudomonase lipase efficiently catalyzes intramolecular transesterification of omega-hydroxyesters to form macrocyclic lactones. The production of the enzymatically active LipL requires a specific molecular chaperon (LimL protein) together with a low-M(r) lipase-activation-factor (LAF) (reduced form of glutathione) for formation of intermolecular disulfide bound
Database
Interpro : No interpro
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : No Pfam
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 1EX9 | Pseudomonas aeruginosa lipase complexed with RC-(RP,SP)-1,2-dioctylcarbamoyl-glycero-3-O-octylphosphonate | pseae-llipa |
| 6CL4 | LipC12 - Lipase from metagenomics | 9bact-g1apt8 |
| 4HS9 | Methanol tolerant mutant of the Proteus mirabilis lipase | promi-c2lfd0 |
| 6JD9 | Proteus mirabilis lipase mutant - I118V\/E130G | promi-c2lfd0 |
| 4GXN | Diethylphosphonate Inhibited Structure of the Proteus mirabilis Lipase | promi-c2lfd0 |
| 4GW3 | Crystal Structure of the Lipase from Proteus mirabilis | promi-c2lfd0 |
| 3W9U | Crystal Crystal structure of Lipk107 | promi-c2lfd0 |
| Gene_locus | Name | Species |
|---|---|---|
| 9gamm-d7nn81 | Acinetobacter sp. XMZ-26 Putative lipase lipA1 Lip26 | Acinetobacter sp. XMZ-26 |
| 9bact-Lipg9 | uncultured bacterium Lipg9 protein | uncultured bacterium |
| 9psed-h2etp9 | Pseudomonas mandelii Organic Solvent-Tolerant Alkaline Lipase EstS | Pseudomonas mandelii |
| 9bact-m1hou7 | uncultured bacterium alkaline lipase obtained from the metagenome of marine sponge Ircinia sp. | uncultured bacterium |
| psecl-LIP3 | Pseudomonas chlororaphis (Pseudomonas aureofaciens): Intracellular lipase EY04_02420 LIP3 | Pseudomonas chlororaphis |
| 9entr-k4k3b3 | Enterobacter sp. Bn-12 lipase | Enterobacter sp. Bn-12 |
| 9gamm-urc15994 | Psychrobacter celer strain PU3 lipase | Psychrobacter celer |
| acicp-AbiS | Acinetobacter baumannii, Triacylglycerol esterase\/lipase EstA, alpha\/beta hydrolase fold\; Lactonizing lipase\; indole synthase A1S_3160 | Acinetobacter baumannii |
| 9gamm-a0a1b1ijp3 | Psychrobacter sp. Lipase ZC12 | Psychrobacter sp. |
| 9bact-LipMF3 | uncultured bacterium Lipase LipMF3 | uncultured bacteria |
| 9psed-c7e3f2 | uncultured Pseudomonas sp. Lipase | uncultured Pseudomonas sp. |
| 9alte-a6f5i7 | Marinobacter algicola DG893 Predicted acetyltransferase and Hydrolase with the alpha\/beta hydrolase fold protein | Marinobacter algicola DG893 |
| 9bact-g1apt8 |  uncultured bacterium LipC12, a true lipase isolated through a metagenomics approach |
uncultured bacterium |
| 9gamm-c0vkc1 | Acinetobacter sp. ATCC 27244 Lipase | Acinetobacter sp. ATCC 27244 |
| 9gamm-q1n359 | Oceanobacter sp. RED65 lactonizing lipase | Oceanobacter sp. RED65 |
| 9gamm-q1ucz4 | Psychrobacter sp. PRwf-1 alpha\/beta hydrolase fold precursor | Psychrobacter sp. PRwf-1 |
| 9gamm-q2c961 | Photobacterium sp. SKA34 putative lactonizing lipase | Photobacterium sp. SKA34 |
| 9gamm-q3nyl5 | Shewanella denitrificans OS217 lactonizing lipase precursor | Shewanella denitrificans OS217 |
| 9gamm-q7x568 | Acinetobacter sp. SY-01 lipase | Acinetobacter sp. SY-01 |
| acica-este3 | Acinetobacter calcoaceticus extracellular lipase, LipA Acinetobacter sp. (strain ADP1) | Acinetobacter calcoaceticus |
| acica-LIPA | Acinetobacter calcoaceticus lipase (EC 3.1.1.3) | Acinetobacter calcoaceticus |
| acilw-d0syz5 | Acinetobacter lwoffii SH145 Predicted protein | Acinetobacter lwoffii SH145 |
| aerhh-a0kfl9 | Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 \/ NCIB 9240) Lipase | Aeromonas hydrophila subsp. hydrophila |
| azovd-c1dli6 | Azotobacter vinelandii (strains DJ \/ ATCC BAA-1303\; AvOP) Alpha\/beta hydrolase fold protein with lipase active site | Azotobacter vinelandii |
| chrvi-CV2714 | Chromobacterium violaceum probable triacylglycerol lipase (EC 3.1.1.3) | Chromobacterium violaceum |
| phoas-q1zm99 | Photobacterium angustum (strain S14 \/ CCUG 15956) (Vibrio sp. (strain S14 \/ CCUG 15956)) putative lactonizing lipase | Photobacterium angustum S14 |
| phopr-q6lfx5 | Photobacterium profundum (Photobacterium sp.), putative lactonizing lipase | Photobacterium profundum |
| promi-c2lfd0 | Proteus mirabilis ATCC 29906 Lipase |
Proteus mirabilis |
| provu-lipas | Proteus vulgaris alkaline lipase gene | Proteus vulgaris |
| pseae-clipa | Pseudomonas aeruginosa cryptic lipase LipC PA4813 | Pseudomonas aeruginosa |
| pseae-llipa | Pseudomonas aeruginosa gene for lactonizing lipase triacylglycerol lipase (EC 3.1.1.3) lip lipA PA2862 PaLipA |
Pseudomonas aeruginosa |
| pseae-q5eue2 | Pseudomonas aeruginosa organic solvent-tolerant lipase (fragment) | Pseudomonas aeruginosa |
| psef5-q4kj24 | Pseudomonas fluorescens (strain Pf-5 \/ ATCC BAA-477)\; Pseudomonas plecoglossicida strain 7 lipase A lipase | Pseudomonas fluorescens |
| psefl-lipa | Pseudomonas fluorescens lipase(lipA) gene [SIK W1, Genomic, 1595 nt] | Pseudomonas fluorescens |
| psefr-lipas | Pseudomonas fragi lips gene for lipase (EC3.1.1.3) | Pseudomonas fragi |
| pseme-LIPA | Pseudomonas mendocina\; Pseudomonas stutzeri\; Pseudomonas xanthomarina\; Pseudomonas sp.\; Streptococcus pneumoniae\; Pseudomonadales bacterium lipa (EC 3.1.1.3) | Pseudomonas mendocina |
| psemy-a4xt61 | Pseudomonas mendocina (strain ymp) Acetyltransferase and hydrolase with the alpha\/beta hydrolase fold-like protein | Pseudomonas mendocina |
| psemy-a4xvf4 | Pseudomonas mendocina triacylglycerol lipase PCLase II | Pseudomonas mendocina |
| psepf-q3kbp5 | Pseudomonas fluorescens (strain PfO-1) alpha\/beta hydrolase fold | Pseudomonas fluorescens |
| psepf-q3kiu1 | Pseudomonas fluorescens (strain PfO-1) alpha\/beta hydrolase fold | Pseudomonas fluorescens |
| psepu-LIP | Pseudomonas putida (strain KT2440) lipase | Pseudomonas putida |
| psesp-lip | Pseudomonas sp. SW-3 putative lipase precursor | Pseudomonas sp. SW-3 |
| psesp-llipa | Pseudomonas species (strain 109) gene for lactonizing lipase | Pseudomonas sp. |
| psewi-LPWA | Pseudomonas wisconsinensis (triglyceride lipase) (tributyrase) | Pseudomonas wisconsinensis |
| psyck-q1qdm7 | Psychrobacter cryohalolentis (strain K5) alpha\/beta hydrolase fold precursor | Psychrobacter cryohalolentis |
| rhofd-q21t36 | Rhodoferax ferrireducens (strain DSM 15236 \/ ATCC BAA-621 \/ T118) triacylglycerol lipase precursor (EC 3.1.1.3) | Rhodoferax ferrireducens |
| shefr-q3nqd4 | Shewanella frigidimarina NCIMB 400 lactonizing lipase precursor | Shewanella frigidimarina NCIMB 400 |
| sorc5-a9grj0 | Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56)) Triacylglycerol lipase | Sorangium cellulosum |
| vibal-q1v8r9 | Vibrio alginolyticus 12G01 lactonizing lipase | Vibrio alginolyticus 12G01 |
| viban-q4zhv3 | Vibrio anguillarum (Listonella anguillarum) llpa | Vibrio anguillarum |
| vibch-lipas | Vibrio cholerae lipase-related lipA formerly hlyC gene | Vibrio cholerae |
| vibpa-VP1181 | Vibrio parahaemolyticus lactonizing lipase | Vibrio parahaemolyticus |
| vibvu-VV12349 | Vibrio vulnificus lactonizing lipase(strain YJ016) VV1990 (strain CMCP6)VV12349 | Vibrio vulnificus |
| psea7-a6vca5 | Pseudomonas aeruginosa (strain PA7). Lipase (Triacylglycerol lipase) | Pseudomonas aeruginosa |
| psea4-u3auv8 | Pseudomonas alcaligenes (strain ATCC 14909 \/ DSM 50342 \/ JCM 20561 \/ NBRC 14159 \/ NCIMB 9945 \/ NCTC 10367 \/ 1577). Putative lipase | Pseudomonas alcaligenes |
| psea4-u3avp1 | Pseudomonas alcaligenes (strain ATCC 14909 \/ DSM 50342 \/ JCM 20561 \/ NBRC 14159 \/ NCIMB 9945 \/ NCTC 10367 \/ 1577). Putative lipase | Pseudomonas alcaligenes |
| Inhibitor | Chemical Nomenclature | Proteins |
|---|---|---|
| Bis(N-propylcarbamic-acid)ethylene-ester | 2-(propylcarbamoyloxy)ethyl N-propylcarbamate | pseae-llipa |
| OCP | [(2R)-2,3-bis(octylcarbamoyloxy)propoxy]-octylphosphinic acid | pseae-llipa burce-lipaa |
| Methyl-DEUP | diethyl (4-methyl-2-oxochromen-7-yl) phosphate | |
| Paraoxon | diethyl (4-nitrophenyl) phosphate | human-PLA2G7 human-PNLIPRP2 humin-cut human-ACHE promi-c2lfd0 yeast-yjg8 bacpu-AXE sulto-ST0071 aspor-cutas pig-1plip fusso-cutas lacrh-B2CZF3 thema-TM0033 colgl-cutas psesp-Q9RBY1 actde-CXE1 myctu-a85c thema-TM0077 rhimi-lipas bacpu-w8fke7 9bact-KP212148 |
| PMSF | Phenylmethylsulfonyl fluoride | theet-q3ch51 emeni-q5avy9 human-ABHD10 human-PREPL myctu-Rv1430 psefl-cxest 9bact-E40 thefu-q6a0i4 ecoli-Aes orysj-Q10QA5 bac25-mglp 9bact-Q0GMU2 bacsu-FENB psepu-clcd1 thema-TM0077 bacsu-RsbQ ecoli-bioh drome-CG9542 human-FAP tubmm-TmEst2 human-NOTUM cutac-e6dae5 9gamm-urc15994 9baci-EstD9 geost-est30 pholl-AntI |
References (8)
| Title : Molecular Characterization of Transesterification Activity of Novel Lipase Family I.1 - Haryati_2022_Biosci.Rep__ |
| Author(s) : Haryati T , Widhiastuty MP , Warganegara FM , Akhmaloka A |
| Ref : Bioscience Reports , : , 2022 |
| Abstract : |
| PubMedSearch : Haryati_2022_Biosci.Rep__ |
| PubMedID: 36111825 |
| Gene_locus related to this paper: pseme-LIPA |
| Title : Structure, mechanism, and enantioselectivity shifting of lipase LipK107 with a simple way - Zhang_2014_Biochim.Biophys.Acta_1844_1183 |
| Author(s) : Zhang L , Gao B , Yuan Z , He X , Yuan YA , Zhang JZ , Wei D |
| Ref : Biochimica & Biophysica Acta , 1844 :1183 , 2014 |
| Abstract : |
| PubMedSearch : Zhang_2014_Biochim.Biophys.Acta_1844_1183 |
| PubMedID: 24602769 |
| Gene_locus related to this paper: promi-c2lfd0 |
| Title : Dieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolution. - Korman_2013_Biotechnol.Biofuels_6_70 |
| Author(s) : Korman TP , Sahachartsiri B , Charbonneau DM , Huang GL , Beauregard M , Bowie JU |
| Ref : Biotechnol Biofuels , 6 :70 , 2013 |
| Abstract : |
| PubMedSearch : Korman_2013_Biotechnol.Biofuels_6_70 |
| PubMedID: 23648063 |
| Gene_locus related to this paper: promi-c2lfd0 |
| Title : Crystal Structure of Proteus mirabilis Lipase, a Novel Lipase from the Proteus\/Psychrophilic Subfamily of Lipase Family I.1 - Korman_2012_PLoS.One_7_e52890 |
| Author(s) : Korman TP , Bowie JU |
| Ref : PLoS ONE , 7 :e52890 , 2012 |
| Abstract : |
| PubMedSearch : Korman_2012_PLoS.One_7_e52890 |
| PubMedID: 23300806 |
| Gene_locus related to this paper: promi-c2lfd0 |
| Title : Lipases for biotechnology - Jaeger_2002_Curr.Opin.Biotechnol_13_390 |
| Author(s) : Jaeger KE , Eggert T |
| Ref : Curr Opin Biotechnol , 13 :390 , 2002 |
| Abstract : |
| PubMedSearch : Jaeger_2002_Curr.Opin.Biotechnol_13_390 |
| PubMedID: 12323363 |
| Title : Crystal structure of pseudomonas aeruginosa lipase in the open conformation. The prototype for family I.1 of bacterial lipases - Nardini_2000_J.Biol.Chem_275_31219 |
| Author(s) : Nardini M , Lang DA , Liebeton K , Jaeger KE , Dijkstra BW |
| Ref : Journal of Biological Chemistry , 275 :31219 , 2000 |
| Abstract : |
| PubMedSearch : Nardini_2000_J.Biol.Chem_275_31219 |
| PubMedID: 10893416 |
| Gene_locus related to this paper: pseae-llipa |
| Title : Glutathione as an essential factor for chaperon-mediated activation of lactonizing lipase (LipL) from Pseudomonas sp. 109 - Tanaka_2000_J.Biochem_127_597 |
| Author(s) : Tanaka J , Nihira T , Yamada Y |
| Ref : J Biochem , 127 :597 , 2000 |
| Abstract : |
| PubMedSearch : Tanaka_2000_J.Biochem_127_597 |
| PubMedID: 10739951 |
| Gene_locus related to this paper: psesp-llipa |
| Title : Bacterial lipolytic enzymes: classification and properties - Arpigny_1999_Biochem.J_343_177 |
| Author(s) : Arpigny JL , Jaeger KE |
| Ref : Biochemical Journal , 343 :177 , 1999 |
| Abstract : |
| PubMedSearch : Arpigny_1999_Biochem.J_343_177 |
| PubMedID: 10493927 |