Tallon LJ

References (12)

Title : Single molecule sequencing and genome assembly of a clinical specimen of Loa loa, the causative agent of loiasis - Tallon_2014_BMC.Genomics_15_788
Author(s) : Tallon LJ , Liu X , Bennuru S , Chibucos MC , Godinez A , Ott S , Zhao X , Sadzewicz L , Fraser CM , Nutman TB , Dunning Hotopp JC
Ref : BMC Genomics , 15 :788 , 2014
Abstract : BACKGROUND: More than 20% of the world's population is at risk for infection by filarial nematodes and >180 million people worldwide are already infected. Along with infection comes significant morbidity that has a socioeconomic impact. The eight filarial nematodes that infect humans are Wuchereria bancrofti, Brugia malayi, Brugia timori, Onchocerca volvulus, Loa loa, Mansonella perstans, Mansonella streptocerca, and Mansonella ozzardi, of which three have published draft genome sequences. Since all have humans as the definitive host, standard avenues of research that rely on culturing and genetics have often not been possible. Therefore, genome sequencing provides an important window into understanding the biology of these parasites. The need for large amounts of high quality genomic DNA from homozygous, inbred lines; the availability of only short sequence reads from next-generation sequencing platforms at a reasonable expense; and the lack of random large insert libraries has limited our ability to generate high quality genome sequences for these parasites. However, the Pacific Biosciences single molecule, real-time sequencing platform holds great promise in reducing input amounts and generating sufficiently long sequences that bypass the need for large insert paired libraries.
RESULTS: Here, we report on efforts to generate a more complete genome assembly for L. loa using genetically heterogeneous DNA isolated from a single clinical sample and sequenced on the Pacific Biosciences platform. To obtain the best assembly, numerous assemblers and sequencing datasets were analyzed, combined, and compared. Quiver-informed trimming of an assembly of only Pacific Biosciences reads by HGAP2 was selected as the final assembly of 96.4 Mbp in 2,250 contigs. This results in ~9% more of the genome in ~85% fewer contigs from ~80% less starting material at a fraction of the cost of previous Roche 454-based sequencing efforts.
CONCLUSIONS: The result is the most complete filarial nematode assembly produced thus far and demonstrates the utility of single molecule sequencing on the Pacific Biosciences platform for genetically heterogeneous metazoan genomes.
ESTHER : Tallon_2014_BMC.Genomics_15_788
PubMedSearch : Tallon_2014_BMC.Genomics_15_788
PubMedID: 25217238
Gene_locus related to this paper: loalo-a0a1i7vgt8

Title : Genome Sequence of Escherichia coli O157:H7 Strain 2886-75, Associated with the First Reported Case of Human Infection in the United States - Sanjar_2014_Genome.Announc_2_e01120
Author(s) : Sanjar F , Hazen TH , Shah SM , Koenig SS , Agrawal S , Daugherty S , Sadzewicz L , Tallon LJ , Mammel MK , Feng P , Soderlund R , Tarr PI , Debroy C , Dudley EG , Cebula TA , Ravel J , Fraser CM , Rasko DA , Eppinger M
Ref : Genome Announc , 2 : , 2014
Abstract : First identified in 1982 as a human pathogen, enterohemorrhagic Escherichia coli of the O157:H7 serotype is a major cause of food-borne acquired human infections. Here, we report the genome sequence of the first known strain of this serotype isolated in the United States.
ESTHER : Sanjar_2014_Genome.Announc_2_e01120
PubMedSearch : Sanjar_2014_Genome.Announc_2_e01120
PubMedID: 24407635
Gene_locus related to this paper: ecoli-fes , ecoli-ycfp , ecoli-ypt1 , ecoli-yqia , ecoli-Z1930

Title : Genomic insights into the emerging human pathogen Mycobacterium massiliense - Tettelin_2012_J.Bacteriol_194_5450
Author(s) : Tettelin H , Sampaio EP , Daugherty SC , Hine E , Riley DR , Sadzewicz L , Sengamalay N , Shefchek K , Su Q , Tallon LJ , Conville P , Olivier KN , Holland SM , Fraser CM , Zelazny AM
Ref : Journal of Bacteriology , 194 :5450 , 2012
Abstract : Mycobacterium massiliense (Mycobacterium abscessus group) is an emerging pathogen causing pulmonary disease and skin and soft tissue infections. We report the genome sequence of the type strain CCUG 48898.
ESTHER : Tettelin_2012_J.Bacteriol_194_5450
PubMedSearch : Tettelin_2012_J.Bacteriol_194_5450
PubMedID: 22965080
Gene_locus related to this paper: mycab-b1mdu3 , mycab-b1mes2 , mycab-b1mes3 , mycab-b1mfj3 , mycab-i9cu79 , mycab-i0pm15 , mycab-i0p5k2 , mycab-h0ig31 , mycab-h0iii8 , myca9-b1miq0 , mycab-r4v1a9

Title : Identification of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli - Moriel_2010_Proc.Natl.Acad.Sci.U.S.A_107_9072
Author(s) : Moriel DG , Bertoldi I , Spagnuolo A , Marchi S , Rosini R , Nesta B , Pastorello I , Corea VA , Torricelli G , Cartocci E , Savino S , Scarselli M , Dobrindt U , Hacker J , Tettelin H , Tallon LJ , Sullivan S , Wieler LH , Ewers C , Pickard D , Dougan G , Fontana MR , Rappuoli R , Pizza M , Serino L
Ref : Proc Natl Acad Sci U S A , 107 :9072 , 2010
Abstract : Extraintestinal pathogenic Escherichia coli (ExPEC) are a common cause of disease in both mammals and birds. A vaccine to prevent such infections would be desirable given the increasing antibiotic resistance of these bacteria. We have determined the genome sequence of ExPEC IHE3034 (ST95) isolated from a case of neonatal meningitis and compared this to available genome sequences of other ExPEC strains and a few nonpathogenic E. coli. We found 19 genomic islands present in the genome of IHE3034, which are absent in the nonpathogenic E. coli isolates. By using subtractive reverse vaccinology we identified 230 antigens present in ExPEC but absent (or present with low similarity) in nonpathogenic strains. Nine antigens were protective in a mouse challenge model. Some of them were also present in other pathogenic non-ExPEC strains, suggesting that a broadly protective E. coli vaccine may be possible. The gene encoding the most protective antigen was detected in most of the E. coli isolates, highly conserved in sequence and found to be exported by a type II secretion system which seems to be nonfunctional in nonpathogenic strains.
ESTHER : Moriel_2010_Proc.Natl.Acad.Sci.U.S.A_107_9072
PubMedSearch : Moriel_2010_Proc.Natl.Acad.Sci.U.S.A_107_9072
PubMedID: 20439758
Gene_locus related to this paper: ecoli-Aes , ecoli-rutD , ecoli-bioh , ecoli-C0410 , ecoli-C2429 , ecoli-C2451 , ecoli-C4836 , ecoli-dlhh , ecoli-entf , ecoli-fes , ecoli-IROD , ecoli-IROE , ecoli-pldb , ecoli-ptrb , ecoli-yafa , ecoli-yaim , ecoli-ybff , ecoli-ycfp , ecoli-ycjy , ecoli-yeiG , ecoli-YFBB , ecoli-yghX , ecoli-yhet , ecoli-yjfp , ecoli-YNBC , ecoli-ypfh , ecoli-yqia , ecoli-Z1930 , ecoli-YfhR , ecout-q1r7l6 , yerpe-YBTT

Title : Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum - Wu_2009_PLoS.One_4_e4207
Author(s) : Wu D , Raymond J , Wu M , Chatterji S , Ren Q , Graham JE , Bryant DA , Robb F , Colman A , Tallon LJ , Badger JH , Madupu R , Ward NL , Eisen JA
Ref : PLoS ONE , 4 :e4207 , 2009
Abstract : In order to enrich the phylogenetic diversity represented in the available sequenced bacterial genomes and as part of an "Assembling the Tree of Life" project, we determined the genome sequence of Thermomicrobium roseum DSM 5159. T. roseum DSM 5159 is a red-pigmented, rod-shaped, Gram-negative extreme thermophile isolated from a hot spring that possesses both an atypical cell wall composition and an unusual cell membrane that is composed entirely of long-chain 1,2-diols. Its genome is composed of two circular DNA elements, one of 2,006,217 bp (referred to as the chromosome) and one of 919,596 bp (referred to as the megaplasmid). Strikingly, though few standard housekeeping genes are found on the megaplasmid, it does encode a complete system for chemotaxis including both chemosensory components and an entire flagellar apparatus. This is the first known example of a complete flagellar system being encoded on a plasmid and suggests a straightforward means for lateral transfer of flagellum-based motility. Phylogenomic analyses support the recent rRNA-based analyses that led to T. roseum being removed from the phylum Thermomicrobia and assigned to the phylum Chloroflexi. Because T. roseum is a deep-branching member of this phylum, analysis of its genome provides insights into the evolution of the Chloroflexi. In addition, even though this species is not photosynthetic, analysis of the genome provides some insight into the origins of photosynthesis in the Chloroflexi. Metabolic pathway reconstructions and experimental studies revealed new aspects of the biology of this species. For example, we present evidence that T. roseum oxidizes CO aerobically, making it the first thermophile known to do so. In addition, we propose that glycosylation of its carotenoids plays a crucial role in the adaptation of the cell membrane to this bacterium's thermophilic lifestyle. Analyses of published metagenomic sequences from two hot springs similar to the one from which this strain was isolated, show that close relatives of T. roseum DSM 5159 are present but have some key differences from the strain sequenced.
ESTHER : Wu_2009_PLoS.One_4_e4207
PubMedSearch : Wu_2009_PLoS.One_4_e4207
PubMedID: 19148287
Gene_locus related to this paper: therp-b9kxz7 , therp-b9l2i8 , therp-b9l396

Title : Candidatus Chloracidobacterium thermophilum: an aerobic phototrophic Acidobacterium - Bryant_2007_Science_317_523
Author(s) : Bryant DA , Costas AM , Maresca JA , Chew AG , Klatt CG , Bateson MM , Tallon LJ , Hostetler J , Nelson WC , Heidelberg JF , Ward DM
Ref : Science , 317 :523 , 2007
Abstract : Only five bacterial phyla with members capable of chlorophyll (Chl)-based phototrophy are presently known. Metagenomic data from the phototrophic microbial mats of alkaline siliceous hot springs in Yellowstone National Park revealed the existence of a distinctive bacteriochlorophyll (BChl)-synthesizing, phototrophic bacterium. A highly enriched culture of this bacterium grew photoheterotrophically, synthesized BChls a and c under oxic conditions, and had chlorosomes and type 1 reaction centers. "Candidatus Chloracidobacterium thermophilum" is a BChl-producing member of the poorly characterized phylum Acidobacteria.
ESTHER : Bryant_2007_Science_317_523
PubMedSearch : Bryant_2007_Science_317_523
PubMedID: 17656724
Gene_locus related to this paper: 9bact-a8djg9

Title : Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters - Wu_2006_PLoS.Biol_4_e188
Author(s) : Wu D , Daugherty SC , Van Aken SE , Pai GH , Watkins KL , Khouri H , Tallon LJ , Zaborsky JM , Dunbar HE , Tran PL , Moran NA , Eisen JA
Ref : PLoS Biol , 4 :e188 , 2006
Abstract : Mutualistic intracellular symbiosis between bacteria and insects is a widespread phenomenon that has contributed to the global success of insects. The symbionts, by provisioning nutrients lacking from diets, allow various insects to occupy or dominate ecological niches that might otherwise be unavailable. One such insect is the glassy-winged sharpshooter (Homalodisca coagulata), which feeds on xylem fluid, a diet exceptionally poor in organic nutrients. Phylogenetic studies based on rRNA have shown two types of bacterial symbionts to be coevolving with sharpshooters: the gamma-proteobacterium Baumannia cicadellinicola and the Bacteroidetes species Sulcia muelleri. We report here the sequencing and analysis of the 686,192-base pair genome of B. cicadellinicola and approximately 150 kilobase pairs of the small genome of S. muelleri, both isolated from H. coagulata. Our study, which to our knowledge is the first genomic analysis of an obligate symbiosis involving multiple partners, suggests striking complementarity in the biosynthetic capabilities of the two symbionts: B. cicadellinicola devotes a substantial portion of its genome to the biosynthesis of vitamins and cofactors required by animals and lacks most amino acid biosynthetic pathways, whereas S. muelleri apparently produces most or all of the essential amino acids needed by its host. This finding, along with other results of our genome analysis, suggests the existence of metabolic codependency among the two unrelated endosymbionts and their insect host. This dual symbiosis provides a model case for studying correlated genome evolution and genome reduction involving multiple organisms in an intimate, obligate mutualistic relationship. In addition, our analysis provides insight for the first time into the differences in symbionts between insects (e.g., aphids) that feed on phloem versus those like H. coagulata that feed on xylem. Finally, the genomes of these two symbionts provide potential targets for controlling plant pathogens such as Xylella fastidiosa, a major agroeconomic problem, for which H. coagulata and other sharpshooters serve as vectors of transmission.
ESTHER : Wu_2006_PLoS.Biol_4_e188
PubMedSearch : Wu_2006_PLoS.Biol_4_e188
PubMedID: 16729848
Gene_locus related to this paper: bauch-q1lsz2

Title : Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote - Eisen_2006_PLoS.Biol_4_e286
Author(s) : Eisen JA , Coyne RS , Wu M , Wu D , Thiagarajan M , Wortman JR , Badger JH , Ren Q , Amedeo P , Jones KM , Tallon LJ , Delcher AL , Salzberg SL , Silva JC , Haas BJ , Majoros WH , Farzad M , Carlton JM , Smith RK, Jr. , Garg J , Pearlman RE , Karrer KM , Sun L , Manning G , Elde NC , Turkewitz AP , Asai DJ , Wilkes DE , Wang Y , Cai H , Collins K , Stewart BA , Lee SR , Wilamowska K , Weinberg Z , Ruzzo WL , Wloga D , Gaertig J , Frankel J , Tsao CC , Gorovsky MA , Keeling PJ , Waller RF , Patron NJ , Cherry JM , Stover NA , Krieger CJ , del Toro C , Ryder HF , Williamson SC , Barbeau RA , Hamilton EP , Orias E
Ref : PLoS Biol , 4 :e286 , 2006
Abstract : The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline and soma functions that are embodied by distinct nuclei within a single cell. The germline-like micronucleus (MIC) has its genome held in reserve for sexual reproduction. The soma-like macronucleus (MAC), which possesses a genome processed from that of the MIC, is the center of gene expression and does not directly contribute DNA to sexual progeny. We report here the shotgun sequencing, assembly, and analysis of the MAC genome of T. thermophila, which is approximately 104 Mb in length and composed of approximately 225 chromosomes. Overall, the gene set is robust, with more than 27,000 predicted protein-coding genes, 15,000 of which have strong matches to genes in other organisms. The functional diversity encoded by these genes is substantial and reflects the complexity of processes required for a free-living, predatory, single-celled organism. This is highlighted by the abundance of lineage-specific duplications of genes with predicted roles in sensing and responding to environmental conditions (e.g., kinases), using diverse resources (e.g., proteases and transporters), and generating structural complexity (e.g., kinesins and dyneins). In contrast to the other lineages of alveolates (apicomplexans and dinoflagellates), no compelling evidence could be found for plastid-derived genes in the genome. UGA, the only T. thermophila stop codon, is used in some genes to encode selenocysteine, thus making this organism the first known with the potential to translate all 64 codons in nuclear genes into amino acids. We present genomic evidence supporting the hypothesis that the excision of DNA from the MIC to generate the MAC specifically targets foreign DNA as a form of genome self-defense. The combination of the genome sequence, the functional diversity encoded therein, and the presence of some pathways missing from other model organisms makes T. thermophila an ideal model for functional genomic studies to address biological, biomedical, and biotechnological questions of fundamental importance.
ESTHER : Eisen_2006_PLoS.Biol_4_e286
PubMedSearch : Eisen_2006_PLoS.Biol_4_e286
PubMedID: 16933976
Gene_locus related to this paper: tetts-i7mam3 , tetts-i7ml33

Title : Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901 - Wu_2005_PLoS.Genet_1_e65
Author(s) : Wu M , Ren Q , Durkin AS , Daugherty SC , Brinkac LM , Dodson RJ , Madupu R , Sullivan SA , Kolonay JF , Haft DH , Nelson WC , Tallon LJ , Jones KM , Ulrich LE , Gonzalez JM , Zhulin IB , Robb FT , Eisen JA
Ref : PLoS Genet , 1 :e65 , 2005
Abstract : We report here the sequencing and analysis of the genome of the thermophilic bacterium Carboxydothermus hydrogenoformans Z-2901. This species is a model for studies of hydrogenogens, which are diverse bacteria and archaea that grow anaerobically utilizing carbon monoxide (CO) as their sole carbon source and water as an electron acceptor, producing carbon dioxide and hydrogen as waste products. Organisms that make use of CO do so through carbon monoxide dehydrogenase complexes. Remarkably, analysis of the genome of C. hydrogenoformans reveals the presence of at least five highly differentiated anaerobic carbon monoxide dehydrogenase complexes, which may in part explain how this species is able to grow so much more rapidly on CO than many other species. Analysis of the genome also has provided many general insights into the metabolism of this organism which should make it easier to use it as a source of biologically produced hydrogen gas. One surprising finding is the presence of many genes previously found only in sporulating species in the Firmicutes Phylum. Although this species is also a Firmicutes, it was not known to sporulate previously. Here we show that it does sporulate and because it is missing many of the genes involved in sporulation in other species, this organism may serve as a "minimal" model for sporulation studies. In addition, using phylogenetic profile analysis, we have identified many uncharacterized gene families found in all known sporulating Firmicutes, but not in any non-sporulating bacteria, including a sigma factor not known to be involved in sporulation previously.
ESTHER : Wu_2005_PLoS.Genet_1_e65
PubMedSearch : Wu_2005_PLoS.Genet_1_e65
PubMedID: 16311624
Gene_locus related to this paper: carhz-metx , carhz-q3abd5 , carhz-q3adp4

Title : Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana - Salanoubat_2000_Nature_408_820
Author(s) : Salanoubat M , Lemcke K , Rieger M , Ansorge W , Unseld M , Fartmann B , Valle G , Blocker H , Perez-Alonso M , Obermaier B , Delseny M , Boutry M , Grivell LA , Mache R , Puigdomenech P , de Simone V , Choisne N , Artiguenave F , Robert C , Brottier P , Wincker P , Cattolico L , Weissenbach J , Saurin W , Quetier F , Schafer M , Muller-Auer S , Gabel C , Fuchs M , Benes V , Wurmbach E , Drzonek H , Erfle H , Jordan N , Bangert S , Wiedelmann R , Kranz H , Voss H , Holland R , Brandt P , Nyakatura G , Vezzi A , D'Angelo M , Pallavicini A , Toppo S , Simionati B , Conrad A , Hornischer K , Kauer G , Lohnert TH , Nordsiek G , Reichelt J , Scharfe M , Schon O , Bargues M , Terol J , Climent J , Navarro P , Collado C , Perez-Perez A , Ottenwalder B , Duchemin D , Cooke R , Laudie M , Berger-Llauro C , Purnelle B , Masuy D , de Haan M , Maarse AC , Alcaraz JP , Cottet A , Casacuberta E , Monfort A , Argiriou A , Flores M , Liguori R , Vitale D , Mannhaupt G , Haase D , Schoof H , Rudd S , Zaccaria P , Mewes HW , Mayer KF , Kaul S , Town CD , Koo HL , Tallon LJ , Jenkins J , Rooney T , Rizzo M , Walts A , Utterback T , Fujii CY , Shea TP , Creasy TH , Haas B , Maiti R , Wu D , Peterson J , Van Aken S , Pai G , Militscher J , Sellers P , Gill JE , Feldblyum TV , Preuss D , Lin X , Nierman WC , Salzberg SL , White O , Venter JC , Fraser CM , Kaneko T , Nakamura Y , Sato S , Kato T , Asamizu E , Sasamoto S , Kimura T , Idesawa K , Kawashima K , Kishida Y , Kiyokawa C , Kohara M , Matsumoto M , Matsuno A , Muraki A , Nakayama S , Nakazaki N , Shinpo S , Takeuchi C , Wada T , Watanabe A , Yamada M , Yasuda M , Tabata S
Ref : Nature , 408 :820 , 2000
Abstract : Arabidopsis thaliana is an important model system for plant biologists. In 1996 an international collaboration (the Arabidopsis Genome Initiative) was formed to sequence the whole genome of Arabidopsis and in 1999 the sequence of the first two chromosomes was reported. The sequence of the last three chromosomes and an analysis of the whole genome are reported in this issue. Here we present the sequence of chromosome 3, organized into four sequence segments (contigs). The two largest (13.5 and 9.2 Mb) correspond to the top (long) and the bottom (short) arms of chromosome 3, and the two small contigs are located in the genetically defined centromere. This chromosome encodes 5,220 of the roughly 25,500 predicted protein-coding genes in the genome. About 20% of the predicted proteins have significant homology to proteins in eukaryotic genomes for which the complete sequence is available, pointing to important conserved cellular functions among eukaryotes.
ESTHER : Salanoubat_2000_Nature_408_820
PubMedSearch : Salanoubat_2000_Nature_408_820
PubMedID: 11130713
Gene_locus related to this paper: arath-MES17 , arath-AT3G12150 , arath-At3g61680 , arath-AT3g62590 , arath-CXE12 , arath-eds1 , arath-SCP25 , arath-F1P2.110 , arath-F1P2.140 , arath-F11F8.28 , arath-F14D17.80 , arath-F16B3.4 , arath-SCP27 , arath-At3g50790 , arath-At3g05600 , arath-PAD4 , arath-At3g51000 , arath-SCP16 , arath-gid1 , arath-GID1B , arath-Q9LUG8 , arath-Q84JS1 , arath-Q9SFF6 , arath-q9m236 , arath-q9sr22 , arath-q9sr23 , arath-SCP7 , arath-SCP14 , arath-SCP15 , arath-SCP17 , arath-SCP36 , arath-SCP37 , arath-SCP39 , arath-SCP40 , arath-SCP49 , arath-T19F11.2

Title : Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana - Theologis_2000_Nature_408_816
Author(s) : Theologis A , Ecker JR , Palm CJ , Federspiel NA , Kaul S , White O , Alonso J , Altafi H , Araujo R , Bowman CL , Brooks SY , Buehler E , Chan A , Chao Q , Chen H , Cheuk RF , Chin CW , Chung MK , Conn L , Conway AB , Conway AR , Creasy TH , Dewar K , Dunn P , Etgu P , Feldblyum TV , Feng J , Fong B , Fujii CY , Gill JE , Goldsmith AD , Haas B , Hansen NF , Hughes B , Huizar L , Hunter JL , Jenkins J , Johnson-Hopson C , Khan S , Khaykin E , Kim CJ , Koo HL , Kremenetskaia I , Kurtz DB , Kwan A , Lam B , Langin-Hooper S , Lee A , Lee JM , Lenz CA , Li JH , Li Y , Lin X , Liu SX , Liu ZA , Luros JS , Maiti R , Marziali A , Militscher J , Miranda M , Nguyen M , Nierman WC , Osborne BI , Pai G , Peterson J , Pham PK , Rizzo M , Rooney T , Rowley D , Sakano H , Salzberg SL , Schwartz JR , Shinn P , Southwick AM , Sun H , Tallon LJ , Tambunga G , Toriumi MJ , Town CD , Utterback T , Van Aken S , Vaysberg M , Vysotskaia VS , Walker M , Wu D , Yu G , Fraser CM , Venter JC , Davis RW
Ref : Nature , 408 :816 , 2000
Abstract : The genome of the flowering plant Arabidopsis thaliana has five chromosomes. Here we report the sequence of the largest, chromosome 1, in two contigs of around 14.2 and 14.6 megabases. The contigs extend from the telomeres to the centromeric borders, regions rich in transposons, retrotransposons and repetitive elements such as the 180-base-pair repeat. The chromosome represents 25% of the genome and contains about 6,850 open reading frames, 236 transfer RNAs (tRNAs) and 12 small nuclear RNAs. There are two clusters of tRNA genes at different places on the chromosome. One consists of 27 tRNA(Pro) genes and the other contains 27 tandem repeats of tRNA(Tyr)-tRNA(Tyr)-tRNA(Ser) genes. Chromosome 1 contains about 300 gene families with clustered duplications. There are also many repeat elements, representing 8% of the sequence.
ESTHER : Theologis_2000_Nature_408_816
PubMedSearch : Theologis_2000_Nature_408_816
PubMedID: 11130712
Gene_locus related to this paper: arath-At1g05790 , arath-At1g09280 , arath-At1g09980 , arath-AT1G29120 , arath-AT1G52695 , arath-AT1G66900 , arath-At1g73750 , arath-AT1G73920 , arath-AT1G74640 , arath-AT1G76140 , arath-AT1G78210 , arath-clh1 , arath-F1O17.3 , arath-F1O17.4 , arath-F1O17.5 , arath-F5I6.3 , arath-At1g52700 , arath-F6D8.27 , arath-F6D8.32 , arath-F9L1.44 , arath-F9P14.11 , arath-F12A4.4 , arath-MES11 , arath-F14G24.2 , arath-F14G24.3 , arath-F14I3.4 , arath-F14O10.2 , arath-F16N3.25 , arath-LCAT2 , arath-At1g34340 , arath-MES15 , arath-CXE6 , arath-ICML1 , arath-At1g72620 , arath-LCAT1 , arath-PLA12 , arath-PLA15 , arath-PLA17 , arath-Q8L7S1 , arath-At1g15070 , arath-SCP2 , arath-SCP4 , arath-SCP5 , arath-SCP18 , arath-SCP32 , arath-SCP44 , arath-SCP45 , arath-SCPL6 , arath-F4IE65 , arath-At1g30370 , arath-T6L1.8 , arath-T6L1.20 , arath-T14P4.6 , arath-MES14 , arath-SCP3 , arath-AXR4 , arath-At1g10040 , arath-ZW18 , arath-pae2 , arath-pae1 , arath-a0a1p8awg3

Title : Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana - Lin_1999_Nature_402_761
Author(s) : Lin X , Kaul S , Rounsley S , Shea TP , Benito MI , Town CD , Fujii CY , Mason T , Bowman CL , Barnstead M , Feldblyum TV , Buell CR , Ketchum KA , Lee J , Ronning CM , Koo HL , Moffat KS , Cronin LA , Shen M , Pai G , Van Aken S , Umayam L , Tallon LJ , Gill JE , Adams MD , Carrera AJ , Creasy TH , Goodman HM , Somerville CR , Copenhaver GP , Preuss D , Nierman WC , White O , Eisen JA , Salzberg SL , Fraser CM , Venter JC
Ref : Nature , 402 :761 , 1999
Abstract : Arabidopsis thaliana (Arabidopsis) is unique among plant model organisms in having a small genome (130-140 Mb), excellent physical and genetic maps, and little repetitive DNA. Here we report the sequence of chromosome 2 from the Columbia ecotype in two gap-free assemblies (contigs) of 3.6 and 16 megabases (Mb). The latter represents the longest published stretch of uninterrupted DNA sequence assembled from any organism to date. Chromosome 2 represents 15% of the genome and encodes 4,037 genes, 49% of which have no predicted function. Roughly 250 tandem gene duplications were found in addition to large-scale duplications of about 0.5 and 4.5 Mb between chromosomes 2 and 1 and between chromosomes 2 and 4, respectively. Sequencing of nearly 2 Mb within the genetically defined centromere revealed a low density of recognizable genes, and a high density and diverse range of vestigial and presumably inactive mobile elements. More unexpected is what appears to be a recent insertion of a continuous stretch of 75% of the mitochondrial genome into chromosome 2.
ESTHER : Lin_1999_Nature_402_761
PubMedSearch : Lin_1999_Nature_402_761
PubMedID: 10617197
Gene_locus related to this paper: arath-At2g45610 , arath-AT2G03550 , arath-AT2G05260 , arath-AT2G12480 , arath-At2g15230 , arath-At2g18360 , arath-At2g19550 , arath-At2g19620 , arath-At2g24280 , arath-AT2G24320 , arath-At2g26740 , arath-At2g26750 , arath-SCP51 , arath-AT2G36290 , arath-At2g42450 , arath-AT2G42690 , arath-AT2G44970 , arath-At2g47630 , arath-AT3g62590 , arath-CGEP , arath-F12L6.6 , arath-F12L6.7 , arath-F12L6.8 , arath-At3g50790 , arath-MES6 , arath-MES7 , arath-MES4 , arath-MES8 , arath-MES2 , arath-MES3 , arath-MES1 , arath-o80731 , arath-pip , arath-PLA11 , arath-PLA13 , arath-PLA16 , arath-PLA19 , arath-q84w08 , arath-SCP8 , arath-SCP9 , arath-SCP10 , arath-SCP11 , arath-SCP12 , arath-SCP13 , arath-SCP23 , arath-SCP26 , arath-SCP28 , arath-SCP46 , arath-T26B15.8 , arath-SCP22 , arath-SFGH , arath-MES19